MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Lixia Luo,Jinglin Shui,Yingying Gu,Dandan Wang,Jiangyang Qin,Yanan Wang,Yong Mei
In order to detect the cervical cancer biomarker squamous cell carcinoma antigen (SCCA) in human serum, an electrochemical immunosensor was constructed. Zirconium 1,4-dicarboxybenzene MOF (UiO-66), with a high specific surface area, was coupled with gold nanoparticles (AuNPs), creating the UiO-66@AuNPs nanomaterial. This coupling increased the sensitivity of the electrochemical immunosensor by providing many biological binding sites for antibodies to bind to Au-S bonds. Then, mesoporous carbon (CMK-3) was added to the UiO-66@AuNPs nanomaterial to obtain a CMK-3@UiO-66@AuNPs nanocomposite. This further increased the electron transfer proficiency of the material and enhanced the electrochemical signal. Finally, the sensitive detection of squamous cell carcinoma antigen (SCCA) was made possible by unique immunological responses between antigens and antibodies. Transmission electron microscopy (TEM) analysis of CMK-3@UiO-66@AuNPs revealed embedded surfaces and voids; cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) confirmed the successful assembly of the electrochemical immunosensor. The developed electrochemical immunosensor demonstrated good linearity (R 1 2 = 0.999) from 0.01 pg/mL to 10,000 pg/mL, which is the concentration range of SCCA in human serum. The limit of detection was 0.0034 pg/mL (at an S/N ratio of 3). The immunosensor exhibited excellent precision, stability and selectivity, with a high rate of recovery (98.26–101.8 %). The experimental validation studies demonstrated that the electrochemical immunosensor was effective for the detection of SCCA in human serum, offering a novel method for the early diagnosis and dynamic surveillance of cervical cancer.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Kexi Sun,Chao Liu,Yi Cao,Jianxia Zhu,Jialin Li,Qing Huang
Background Rapid and accurate detection of glutathione content in human blood plays an important role in real-time tracking of related diseases. Currently, surface-enhanced Raman scattering/spectroscopy (SERS) combined with nanozyme material has been proven to have excellent properties in the detection applications compared to many other methods because of it combines the advantages of trace detection capability of SERS and efficient catalytic activity of nanozymes. However, there are still existing problems in real sample detection, and to achieve quantitative detection is still challenging. Results In this study, gold nanoparticles (AuNPs) were synthesized in situ on the surface of two-dimensional Cu-porphyrin metal-organic framework (MOF) nanosheets to produce the AuNPs@Cu-porphyrin MOF nanozyme, which exhibited both oxidase-like activity and SERS detection ability. On one hand, the intrinsic oxidase-like activity of the nanozyme could be inhibited due to the chelation of glutathione (GSH) and Cu, which thus led to the visual color change of the solution. On the other hand, the abundant Raman “hot spots” at the nanogap generated by Au NPs and the internal standard (IS) signal provided by Cu- meso -tetra (4-carboxyphenyl) porphine (Cu-TCPP) MOF improved the sensitivity and quantitative accuracy of detection. Significance and novelty A dual-mode signal output sensor based on the nanozyme was thus established, which could be used in the trace detection of GSH. Such a dual-mode sensor possesses excellent detection performance, with the advantage of both wide detection range from 1 to 300 μM in the colorimetric detection mode and high sensitivity with LOD of 5 nM in the SERS detection mode, and can be applied to GSH detection in actual serum samples with reliable results.
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PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Nanfei Yang,Yuncheng Bei,Yahong Huang,Wei Zheng,Jiehua Ma,Jiangqiong Ke
Serum Cystatin C (CysC) is an impressive marker for early diagnosis of renal dysfunction. In this work, we established a novel electrochemical immunosensor based on Fe 3 O 4 /AuNPs-MWCNTs@PDA nanocomposite for the detection of CysC. The Fe 3 O 4 /AuNPs-MWCNTs@PDA nanozyme complex by polydopamine encapsulation can not only carry massive detection antibodies, but also bind the electroactive substance toluidine blue (TB) through electrostatic adsorption. By immobilizing AuNPs onto the electrode to bind the capture antibody (Ab1), we constructed a sandwich electrochemical immunosensor with low cost, high sensitivity, and repeatability. The detection range is 3.9–125.0 ng/mL with a significant linear relationship between the current peak difference (ip) and logarithm of the CysC concentration. Moreover, the detection limit of the immunosensor is 0.157 ng/mL. We have successfully utilized this novel immunosensor to detect CysC in human serum samples, and these results have implications for its potential use in clinical application.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Jintao Liang,Qing Huang,Liang Wu,Xiaohang Shi,Kaiteng Yan,Fei Guo,Zhide Zhou,Guiyin Li
Glucose (Glu) is an important source of energy and metabolic intermediate for animal and plant cells. In this study, a high-performance biosensor for specific detection of Glu was constructed by combining a light-addressable potentiometric biosensor (LAPS) and a specific sensitive unit. The specific sensitive unit was adopted a silicon-based capacitive structure, and the glucose oxidase/reduced graphene oxide -chitosan-ferrooxidase/gold nanoparticles (GOX/RGO-CS-Fc/AuNPs) was modified onto the silicon-based working electrode to construct an nanosensing membrane. The interaction between the specific sensitive unit and Glu resulted in the formation of a bilayer potential, and the field effect generated by the bilayer potential caused a change in the capacitance of the silicon structure, which resulted in a change in the photocurrent-voltage characteristics of the LAPS. Through the potential shift in the photocurrent-voltage characteristics, the LAPS was capable of detection the concentration of Glu. Under optimal conditions, the potential shift was linearly correlated with the Glu concentration from 0.01 mg/mL to 4.00 mg/mL with R 2 of 0.9821, and the limited detection was 0.001 mg/mL. In addition, the LAPS showed good recovery (97.92 %–105.72 %), admirable specificity and stability in detection Glu in human serum samples.
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ELECTROCHIMICA ACTA
·
2017
·
IF 4.798
Shiqiao Wang,Guohan Sun,Zuanguang Chen,Yawen Liang,Qing Zhou,Yufang Pan,Haiyun Zhai
A sensitive and highly selective imprinted sensor for detecting an oxidative stress biomarker 3-nitrotyrosine (3-NT) detection was developed based on electropolymerized pyrrole molecularly imprinted polymer (MIP) doped with gold nanoparticles (AuNPs) on a glassy carbon electrode (GCE) modified with activated multiwall carbon nanotube @ graphene oxide nanoribbons (AMWCNT@GONRs). Adding AuNPs into the polymer composite effectively generated electrical response by facilitating the charge transfer process. As the specific recognition sites, the fabricating imprinted polymer-AMWCNT@GONRs composite exhibited excellent sensitivity, stability and specific selectivity behavior towards 3-NT. The surfaces morphology of the MIP modified electrode was characterized by scanning electron microscopy (SEM) showing that AMWCNT@GONRs were well dispersed under the imprinted polymer owning to highly porous surface structure. Under optimal conditions, the determination range of imprinted sensor for 3-NT was linearly response from 0.2 to 50.0 μM with a detection limit (S/N = 3) of 50.0 nM. Furthermore, this modified MIP sensor was successfully used for detection of 3-NT in human serum and urine samples.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Fuxin Liu,Wenyan Li,Fang Nian,Haitao Long,Lumei Pu,Xiuhui Liu
Homocysteine (Hcy) is a key biomarker for various diseases, and its precise detection is essential for early clinical diagnosis. Herein, an innovative ratiometric electrochemical aptasensor was constructed to overcome the limitations in sensitivity, selectivity, and stability in conventional Hcy detection methods. Firstly, we synthesized a novel 3D MXene/rGO aerogel loaded AuNPs composite (Au/MGA-40) with outstanding specific surface area and remarkable electrical conductivity. Next, the Hcy aptamer was self-assembled to the Au/MGA-40/MB modified electrode surface by gold sulfur bonds (Au-S). Finally, a ratiometric detection mode was established based on the linear relationship between the oxidation peak current ratios (I Hcy /I MB ) and the Hcy concentrations. The aptasensor exhibited excellent analytical behavior for Hcy, with a ultrawide detection range from 2.14 nM to 200 μM and a detection limit as low as 0.835 nM. It also displayed remarkable selectivity due to the specific recognition between Hcy and the aptamer, enabling discrimination of Hcy from structurally similar molecules such as cysteine (Cys), glutathione (GSH) and methionine (Met). The ratio signal mode demonstrated superior long-term stability performance compared to conventional single signal sensors. As evidenced by the successful detection of Hcy in human serum, which underscores its potential for early screening of Hcy-related diseases.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yang Liu,Zixin Lin,Yuetao Zhang,Xinyu Zhang,Zhiqi Li,Rubing Xiong,Chunxiao Long,Jiaqi Huang,Can Liu,Ziwei Zheng,Dan Li,Yongchang Ouyang,Lei Mou
In view of the need for real-time, multimodal respiratory health monitoring, we present a wearable sensor platform (BreathFIT) that simultaneously measures lactate and electromyography (EMG) signals for assessment of diaphragm function. By virtue of integrating liquid metal-polydimethylsiloxane (LM-PDMS) flexible sticky conductors for EMG acquisition with a lactate screen-printed electrode (LSPE), the platform enables concurrent measurement of muscle electrical activity and metabolic byproducts (lactate) from the diaphragm. Data are captured and visualized in real time via custom software, enabling machine learning models (LSTM, DNN, SVM, Random Forest) to classify diaphragm activity patterns during rest, exercise, and sleep. Owing to its integrated design, the system exhibits stable electrochemical performance, exceptional mechanical compliance (300 % stretchability), excellent skin conformality (39 N cm −1 adhesion strength), and high accuracy in distinguishing diaphragm states. Consequently, all models demonstrate high classification accuracy, with Random Forest proving the most effective at capturing time-series features (accuracy = 92.19 %). The dual-modality platform uniquely integrates electrical and biochemical sensing with intelligent analysis. This configuration enables high spatiotemporal-resolution monitoring of respiratory muscle activity. Consequently, the system opens a new avenue for wearable multimodal health monitoring, providing significant potential for both clinical and at-home respiratory health management.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Jiwei Dong,Guangxian Wang,Lingjun Geng,Haifang Wang,Chengqiang Li,Rui Xu,Jing Liu,Jingjing Liu,Ibrahim A. Darwish,Xia Sun,Yemin Guo,Jicheng Zhao
To address the threats posed by organophosphorus pesticides (OPs) to human health and the environment, this study developed a highly sensitive electrochemical aptasensor based on gold nanoparticles (AuNPs) and a functionalized bimetallic copper-zirconium metal-organic framework (CuZr-MOF). In this system, Cu + doping greatly enhances electrical conductivity, after which Zr⁴⁺ provides excellent stability and a large specific surface area. The combination of AuNPs and CuZr-MOF formed a nanocomposite that enhanced sensitivity and enabled the stable immobilization of a thiol-modified broad-spectrum aptamer via Au-S bonding, facilitating efficient recognition of OPs. The aptasensor demonstrates exceptional performance across a concentration range of 1×10 -12 to 1×10 -6 M, with limits of detection of 2.36×10 -4 pM, 4.56×10 -2 pM, and 1.20×10 -2 pM for three representative OPs, and recovery rates ranging from 91.0% to 101.2%. The method’s reliability is further validated by liquid chromatography-tandem mass spectrometry (LC-MS/MS). This study presents a simple and effective approach for detecting OPs in vegetables, demonstrating significant potential for on-site food safety monitoring.
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ELECTROCHIMICA ACTA
·
2024
·
IF 5.5
Yuhao Zhang,Luting Yan,Lei Tian,Qi Guo,Maosheng Sang,Shouyi Dou,Falan Li,Qingqing Yang,Xia Sun,Yemin Guo,Yanyan Zhang
Vibrio parahaemolyticus ( VP ) is one of the most common pathogens in aquatic products, and its study using electrochemiluminescence is still relatively rare. In this study, an electrochemiluminescent (ECL) biosensor was developed to detect VP in aquatic products using AuNPs modified PEI/MWCNTs (AuNPs@PEI/MWCNTs) as a substrate material and ordered mesoporous carbon (OMC) adsorbed Ru(bpy) 3 2+ (OMC@Ru(bpy) 3 2+ ) as a luminescent material. To increase the conductivity of the biosensor and offer a favorable loading environment for the immobilization of the luminescent probe, AuNPs@PEI/MWCNTs are employed as the substrate. Meanwhile, the OMC@Ru(bpy) 3 2+ functionalized DNA probe acts as a luminescent and capture probe to generate ECL signals and specifically recognize different concentrations of VP . The biosensor has good selectivity and stability and has been successfully applied to the detection of VP in scallops. This method not only expands the potential uses of bioprobe-based ECL biosensors, but also offers a new and effective approach used to detect food-borne pathogens in aquatic products.
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高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 8
·1-11
·
IF 5.833
Zhang Youxiong,Li Bing,Wei Xianhu,Gu Qihui,Chen Moutong,Zhang Jumei,Mo Shuping,Wang Juan,Xue Liang,Ding Yu,Wu Qingping
A facile and versatile competitive electrochemical aptasensor for tobramycin (TOB) detection is described using electrochemical-deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework (AuNPs/P-MOF) as signal-amplification platform and a DNA probe labeled with methylene blue (MB) at the 3′-end (MB-Probe) as a signal producer. First, F-Probe (short complementary DNA strands of both the aptamer and the MB-Probe label with a sulfhydryl group at the 5′-end) was immobilized on the AuNPs/P-MOF modified electrode as detection probes, which competed with TOB in binding to the aptamer. TOB-aptamer binding resulted in F-Probe remaining unhybridized on the electrode surface, so that a significant current response was generated by hybridizing with MB-Probe instead. The developed strategy showed favorable repeatability, with a relative standard deviation (RSD) of 4.3% computed over five independent assays, and high stability, with only 6.8% degradation after 15 days of storage. Under optimal conditions, the proposed aptamer strategy exhibited a linear detection range from 100 pM to 500 nM with a limit of detection (LOD) of 56 pM (S/ N = 3). The electrochemical aptasensor demonstrated remarkable selectivity, and its feasibility for accurate and quantitative detection of TOB in milk samples was confirmed (RSD < 4.5%). Due to its simple design, easy operation, and high sensitivity and selectivity, the proposed method could expect to detect other antibiotics by replacing the aptamers. In summary, this study provides a simple and effective new strategy for electrochemical aptasening based on MOF-based sensing interface. Graphical abstract
Scheme illustration of label-free competitive electrochemical aptamer-based detection of tobramycin based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework as signal-amplification platform.
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高影响因子
Nanoscience and Nanotechnology Letters
·
2019
·Vol. 11
·期 5
·638-644
·
IF 0
Jiang Pengfei,Haji Chomba,Ye Xiufang,Chang Menglei,Li Wenjun,He Xuliang,Chen Dongchu,Nie Libo
An inductive coupled plasma mass spectrometry (ICP-MS) method based on gold nanoparticles (AuNPs) and bio-barcode signal amplification was presented for the sensitive detection of gastric cancer related gene. The target DNA, magnetic nanoprobes and SiO2/AuNPs barcode probes
were hybridized to form a "sandwich" structure, then a large amount of AuNPs which were amplified by bio-barcode technology were detected by ICP-MS. This method presented a limit of detection as low as 1 fM. The ratio of the background-subtracted 197 Au signals for totally complementary
DNA, single-base mismatched DNA, double-base mismatched DNA and totally mismatched DNA was 115:30:16:1, respectively. It's suggested that the complementary and mismatched DNA can be distinguished clearly. This novel ICP-MS biosensor is prospective in DNA detection with high sensitivity and
specificity.
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ELECTROANALYSIS
·
2023
·Vol. 35
·期 9
·e202300064
·
IF 3
Jinjin Zhang,Wenjing Liu,Qi Wang,Jianying Qu
In this work, two-dimensional cobalt metal organic framework nanosheets ( CoTP ) were prepared by the simple hydrothermal reaction of cobalt nitrate hexahydrate with terephthalic acid, and then calcined at a high temperature under the environment of nitrogen to obtain cobalt carbon nanomaterials ( Co-CNs ). The gold nanomaterials ( AuNPs ) were prepared by redox reaction. The prepared materials were characterized and analyzed by XRD, SEM and IR. A novel electrochemical sensor ( AuNPs/Co-CNs ) was constructed successfully on a glassy carbon electrode combining Co-CNs with AuNPs , which showed significant electrocatalytic activities for acetaminophen ( PA ) and 4-aminophenol ( 4-AP ) and could be used to detect them simultaneously. Under the optimal conditions, the linear ranges for determination of PA and 4-AP were 0.50–250.00 μM and 0.50–350.00 μM with the detection limits of 0.11 μM and 0.10 μM, respectively. Compared with other related reports, the proposed sensor exhibits a wider linear ranges, good sensitivity, accuracy and selectivity with potential applications in real sample analysis.
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高影响因子
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