FOOD CHEMISTRY
·
2020
·
IF 6.306
Shufen Wu,Xiaochan Wang,Yuxin Bao,Chuanying Zhang,Huanhuan Liu,Zhenjing Li,Mianhua Chen,Changlu Wang,Qingbin Guo,Xin Peng
Monascin (MS) is a yellow lipid-soluble azaphilonoid pigment identified from Monascus -fermented products with promising biological activities. This work studied interactions between MS and bovine serum albumin (BSA) as well as their influences on the antioxidant activity of MS. Experimental results demonstrated that the fluorescence emission of BSA was quenched by MS via static quenching mechanism and the formed BSA-MS complex was mainly maintained by hydrophobic and hydrogen bond interactions. Meanwhile, the probable binding pocket of MS located near site I of BSA and the corresponding conformational and structural alterations of BSA were determined. Furthermore, the molecular modeling approach was performed to understand the visual representation of binding mode between BSA and MS. It was noticeable that the BSA-MS complex exhibited reduced DPPH radical-scavenging ability, which might be attributed to the restraining effect of BSA on the relevant reaction pathways involved in antioxidation by MS.
研究领域:
生物化学
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食品科学
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分子生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Lijing Xu,Kongyin Zhao,Junping Miao,Zhenhao Yang,Zhiwei Li,Lei Zhao,Hongxian Su,Ligang Lin,Yunxia Hu
In order to solve the problems of poor mechanical property, non-antibacterial and low flux of calcium alginate (CaAlg) membrane, silver nanoparticles (AgNPs) were synthesized with bovine serum albumin (BSA) and carboxymethyl chitosan (CMCS) for improving CaAlg membrane in this paper. Meanwhile, the dispersion property of silver nanoparticles and the mechanical property, thermal stability , antibacterial property and filtration efficiency of the composite membrane were explored. The results illustrated CMCS observably strengthened the mechanical property and thermal stability of the composite membrane, and AgNPs endowed the composite membrane with excellent antibacterial property . The flux of the BSA/CMCS/AgNPs/CaAlg composite membrane was raised compared to CaAlg membrane. Finally, the viscose fiber/polyethylene terephthalate fiber (VF-PET) nonwoven fabric was introduced as the support layer to further improve the filtration flux and mechanical property of the composite membrane. VF-PET/BSA/CMCS/AgNPs/CaAlg membrane had a rejection rate of over 99.0 % for dye molecules and <9.0 % for salt ions, while the flux maintained 38.5 L·m −2 ·h −1 . Furthermore, VF-PET/BSA/CMCS/AgNPs/CaAlg membrane also had excellent separation effect on actual dye wastewater. The separation of dye and salt by the membrane mainly depended on the screening mechanism of membrane pore size, rather than adsorption. The composite membrane had an outstanding performance on the separation of dye molecules and inorganic salt ions.
研究领域:
材料科学
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环境科学
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生物医学工程
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2018
·
IF 3.997
Jie Zhang,Xiuli Ren,Xiaohan Tian,Peng Zhang,Zhenhua Chen,Xun Hu,Xifan Mei
Developing safe and effective stimuli-responsive nanocarriers is very important for tumor chemotherapy. In this work, bovine serum albumin (BSA) and green tea polyphenol (TP) were used to prepare glutathione (GSH) and enzyme (trypsin) responsive nanocarriers for doxorubicin (DOX). These nanocarriers were further modified with folate, briefly named as DOX@BSA-TP-FA NSs. The diameter of nanocarriers was about 220 nm. The DOX loading efficiency and loading amount were 86.4% and 23.5 wt%, respectively. The cellular uptake, apoptosis, and GSH and trypsin responsive release properties of these nanocarriers were investigated.
研究领域:
生物医学工程
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肿瘤学
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药剂学
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DOI
PubMed
高影响因子
CYTOTECHNOLOGY
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2011
·Vol. 64
·期 2
·157-163
·
IF 1.277
Yi Yu,Wang Zhuhuan,Li Min,Zhu Keyin,Ying Guoqing
Monoclonal antibodies (McAbs) against chloramphenicol (CAP) were produced to detect CAP residues, which could be toxic and possesses a potential threat to human health. The CAP-BSA conjugate was obtained by bovine serum albumin (BSA) coupled with CAP, and used to immunize the mice. The splenocytes from the immunized mice were fused with mouse myeloma cells SP2/0 to form hybridoma, which may secrete McAbs against CAP. Hybridomas 1D 1 and 3G 12 secreting McAbs against CAP were obtained by screening. Ascites containing McAbs were prepared by injecting 1 x 10 6 cells of hybridoma 1D 1 and 3G 12 into the abdomen of mice. Protein A affinity chromatography was used to purify McAbs against CAP in a single chromatographic step with recovery yield above 80% and purity above 95% and full recovery of antibody activity. Experiments showed that McAb 3G 12 was highly specific for CAP and had no cross-reactivity with analogues which have a structure similar to CAP. The IC 50 value was 50.8 ng/mL.
研究领域:
免疫学
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药理学
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毒理学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Lei Meng,Chengwu Lan,Zhonghu Liu,Na Xu,Yuqing Wu
It is of great importance to detect chlorotetracycline (CTC) in a highly sensitive and specific way because of its wide distribution in aquaculture and animal husbandry. Herein, we propose a novel ratiometric fluorescence strategy to assay CTC by using bovine serum albumin stabilized gold nanoclusters (BSA-AuNCs). The BSA-AuNCs consisting of 25 gold atoms (Au 25 NCs) display a red emission at 640 nm ( λ ex = 370 nm). In the presence of CTC, a new blue emission at 425 nm is emerged and its intensity dramatically increases with the addition of more the analyte; meanwhile the red emission at 640 nm shows a linear decrease reversely. However, at identical conditions neither the analogues of CTC as tetracycline (TC), oxytetracycline (OTC) or doxycycline (DC) induces similar response of BSA-AuNCs. Such interesting phenomenon is proven related to the conversion from large Au 25 NCs to smaller nanoclusters composing 8 gold atoms (Au 8 NCs), which intrinsically originate from the interaction between CTC and the ligand BSA. Therefore, a ratiometric probe is established to sensitively detect CTC in the wide range (0.2–10 μM) with a low limit of detection (LOD) at 65 nM. In addition, this strategy can also be applied to assay CTC in human serum, showing great promise for practical applications in future.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
OPTICAL MATERIALS
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2021
·
IF 3.08
Yu Chen,Ting Feng,Li Chen,Yiming Gao,Junwei Di
A “signal-on” fluorescence method for determination of hydrogen peroxide (H 2 O 2 ) and glucose was developed using bovine serum albumin-capped Ag nanoclusters with thirty atoms (BSA-Ag 30 NCs) as sensing probe. It was relied upon the size-dependent fluorescence of BSA-AgNCs. The prepared BSA-Ag 30 NCs have an average diameter of ~3.4 nm and exhibit a red emission at 635 nm. The fluorescence intensity of BSA-AgNCs could be enhanced by H 2 O 2 with the etching and then formation of smaller nanoclusters. Under the optimum experimental conditions, a linear calibration curve for H 2 O 2 was obtained within the concentration range of 2–200 μM with detection limit of 1.1 μM. Coupling with glucose oxidase (GOD) catalytic reaction, the BSA-Ag 30 NCs sensing platform could also be used to detect glucose. The enhancement of fluorescence intensity (F–F 0 )/F 0 was linear related to the concentration of glucose over the range 5–180 μM with detection limit of 3.4 μM. This method was successfully applied to the determination of glucose in human serum samples with satisfactory result.
研究领域:
分析化学
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纳米技术
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生物化学
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2018
·
IF 3.927
Di Zhou,Guolong Rong,Sa Huang,Jinhui Pang
In order to mitigate the selectivity-permeability trade-off for ultrafiltration membranes, a series of polyphenylene sulfone (PPSU) with flexible negative charged sulfonated side chains (SPPSU-SC) were synthesized applied in ultrafiltration (UF) field. And tests results showed that the ultrafiltration performance was enhanced greatly. In short, the water flux increased to 380 L · m −2 · h −1 at the sulfonation degree of 20%, which was as two and a half times high as PPSU membranes. However, the membrane SPPSU-SC20 still held a relatively high BSA rejection of 91.6%, which should be attributed to the enrichment of negative charged sulfonic groups on the membrane surface. More importantly, the antifouling properties were also promoted. The total fouling ratio (R t ), reversible fouling ratio (R r ), irreversible fouling ratio (R ir ) and flux recovery ratio (FRR) were all improved to some degree. Besides, compared to the main-chain sulfonated PPSU with the sulfonation degree of 20% (SPPSU-MC20) membranes, SPPSU-SC20 had a better separation structure caused by better phase separation during the membrane preparation period at the sane high sulfonation degree. In a word, the results showed that side-chain sulfonated PPSU possessed a potential bright horizon in ultrafiltration field.
研究领域:
材料科学
·
化学工程
·
膜技术
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DOI
高影响因子
Food & Function
·
2022
·Vol. 13
·期 17
·8920-8929
·
IF 6.317
Yao Hu,Chaojun Guo,Qianzhu Lin,Jiali Hu,Xiaojing Li,Shangyuan Sang,David Julian McClements,Jie Long,Zhengyu Jin,Jinpeng Wang,Chao Qiu
Curcumin shows poor bioaccessibility due to its poor water solubility, which limits its application in aqueous formulations, and the weak binding to plasma proteins, which hinders its transportation to targeted sites through the systemic circulation. In this study, we explored the potential of a cyclodextrin derivative, succinic acid-β-cyclodextrin (SACD), to overcome these problems. Complexation of curcumin to SACD significantly increased its water-dispersibility and enhanced its binding to a model plasma protein: bovine serum albumin (BSA). The binding constant and number of binding sites of the ligand–protein system increased from 3.87 × 103 to 1.52 × 105 L mol−1 s−1 and from 0.80 to 1.15 in the absence and presence of SACD, respectively. The presence of SACD also reduced the ability of curcumin to promote conformational changes in BSA. For instance, the thermal denaturation temperatures of BSA in BSA-curcumin mixtures was 65.3 °C and 67.0 °C in the absence and presence of SACD, respectively, compared to 67.8 °C for pure BSA. These effects were attributed to the fact that SACD forms an inclusion complex with curcumin and modulates the binding of curcumin with BSA, which may affect the biological accessibility of curcumin in the systemic circulation.
研究领域:
药学
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生物化学
·
药物递送系统
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
N.N. Zhen,K.H. Chen,M.T. Li,F. Wen,Q.Y. Deng
Ultra-high-molecular weight polyethylene (UHMWPE) is the most widely used friction material for artificial joints. Polyethylene debris generated during its use is the main reason behind the aseptic loosening of artificial joints. Improving the wear resistance of UHMWPE and reducing debris generation have been the focus of research in this field. Inspired by the structure of natural cartilage and based on our previous research, we developed a new type of porous UHMWPE-based artificial joint material doped with copper-containing carbon dots (Cu-CDs). In a protein-containing lubricating medium, this material exhibited auto-antifriction properties by absorbing/releasing the lubricating medium and forming a protein film on the tribo-interface. These effects can significantly enhance the wear resistance of UHMWPE. The results showed that spherical Cu-CDs with an average size of 5–6 nm were successfully prepared. The tensile strength of the porous UHMWPE doped with Cu-CDs (Cu-CDs@UHMWPE) (approximately 9.93 MPa) was close to that of natural joint cartilage. Cu-CDs@UHMWPE promoted the adsorption of proteins by the friction interface and formed a protein film that reduced wear. Under the same friction conditions, the wear index of Cu-CDs@UHMWPE was approximately three times lower than that of pure UHMWPE. This study showed that UHMWPE can be imbued with the ability to form protein films to enhance wear resistance, and these findings provide experimental and theoretical evidence for the development of novel biomimetic artificial joint materials.
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Food Chemistry-X
·
2026
·
IF 9.2
Mahmoud Salah,Mohamed F. Hassan,Ibrahim Khalifa,Mohammed Mansour,Bokai Hu,Yuanyuan Liu,Qiujin Zhu
The gel matrix controlled the release of flavonoid extracts. • Improving gel texture properties with flavonoids for improved bioactivity. • The synthesized gel showed an inhibitory effect on L. monocytogenes. • The synthesized gel showed an inhibitory effect on A. westerdijkiae. • The synthesized gel preserved chicken breast quality. This study developed bovine serum albumin (BSA)-curdlan (Cn) gels infused with flavonoid extract (Fs) from orange peels to enhance chicken breast's quality. Characterization via fluorescence intensity (FI), FTIR, UV–vis, SEM, and XPS confirmed effective flavonoid integration and stable structure. Among variants, the BSA-Cn-Fs-9 gel showed the highest antioxidant activity (DPPH 40.30% and ABTS 93.72%) and sustained the release rate of Fs extract (72.91%). BSA-Cn-Fs-9 gel showed strong inhibitory effects against bacteria, including S. aureus (12.7 mm) , B. cereus (13.4 mm) , E. coli (11.4 mm), and L. monocytogenes (16 mm) , and against fungi, including P. expansum (35.7%) and A. westerdijkiae fc-1 (57.44%). Applied to chicken, BSA-Cn-Fs-9 gel inhibits microbial growth, maintains a pH of 6.5, and reduces lipid oxidation (TBRAS at 0.29 mg/kg). The findings showed that the synthesized gel could serve as an eco-friendly gel material with strong potential for practical food safety monitoring and for reducing food contamination.
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DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 3
·122528
·
IF 7.5
Yu Gao,Linan Zhou,Qingqing Peng,Tingting Cui,Ziyi Guan,Fang Zhang,Li Wang,Huiyi Yang,Fang Chai
Multiple trace heavy metal pollutants present a serious threat to both the environment and ecosystems, and their coexistence also affected the detection efficiency. Therefore, multi-mode responsive optical probes with versatile application prospects for optical sensing are highly desired. Here, yellow-emissive carbon dots (Y-CDs) with aggregation-induced emission (AIE) characteristics were fabricated via a hydrothermal approach, boasting a absolute quantum yield of 13.88% and a fluorescence lifetime of 8.55 μs. Leveraging the AIE behavior and quenching effect, Y-CDs were successfully achieved the multivariate detection of Ag + , Hg 2+ separately, and further realized cascade sensing BSA, with detection limits of 5.59 μM, 7.7 nM, and 0.9 μM respectively. Subsequently, a portable smartphone-based sensing platform was developed to facilitate rapid on-site analysis, and a logic gate was designed to expedite integration into intelligent devices. Furthermore, Y-CDs demonstrated remarkable antibacterial action via in vitro antibacterial assays, displaying significant inhibitory effects against both E. coli and S. aureus , providing the possibilities for testing in the food and medical fields with high standards of hygiene permits. With their exceptional multifunctional sensing capabilities, Y-CDs were expected holding extensive promising application prospects in the realm of real-time monitoring of environmental and food safety.
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DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Yinqiang Xia,Jin Zhao,Zheying Gao,Yifei Ruan,Siqi Wang,Liangbin Hu,Tianli Yue
Matrix interference poses persistent and significant challenges against rapidly detecting gentamicin residue in foods. Here, we fabricated synergistic antifouling surfaces using the phase transition of bovine serum albumin (PTB) and hyaluronic acid (HA) on substrates. The hybrid PTB + HA coatings showed ultralow adsorption of BSA and lysozyme, requiring 10 times less PTB dosage. With PTB's assistance, the modification of HA proved to be much more cost-effective than the sulfhydrated HA. The adsorption of E. coli on the hybrid coatings could be reduced by 92.6 %, illustrating good properties of antibacterial adsorption. Furthermore, the hybrid surfaces demonstrated good stability despite being rinsed with strong acid and alkaline solutions. Based on the immune-inhibition assay, the sensitive antifouling sensors were developed with a low detection limit (1.8 ng/mL for gentamicin) and satisfactory recovery rate (94.0 %–104.0 %) in goat milk. All tests could be completed within 10 min, confirming the sensor system's feasibility and efficiency.
研究领域:
生物化学
·
材料科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）