Neural Regeneration Research
·
2024
·
IF 5.9
Fu Lanya,Hu Xiaofang,Xu Jiawei,Li Zhenlin,Cai Jiale,Ma Xinrui,Zou Ying,He Ye,Xu Shuyi,Xu Yizhou,Zhang Jiaqi,Li Yunlun,Liu Jingmin,Fong Tsz Hei,Wang Xianghai,Zhu Lixin,Chen Dongfeng,Liu Aijun,Ma Xiaodong,Guo Jiasong
Our recent study demonstrated that knockout of microRNA-301a attenuates migration and phagocytosis in macrophages. Considering that macrophages and Schwann cells synergistically clear the debris of degraded axons and myelin during Wallerian degeneration, which is a prerequisite for nerve regeneration, we hypothesized that microRNA-301a regulates Wallerian degeneration and nerve regeneration via impacts on Schwann cell migration and phagocytosis. Herein, we found low expression of microRNA-301a in intact sciatic nerves, with no impact of the microRNA-301a knockout on nerve structure and function. By contrast, we found significant upregulation of microRNA- 301a in injured sciatic nerves. We established a sciatic nerve crush model in microRNA-301a knockout mice, which exhibited attenuated morphological and functional regeneration following sciatic nerve crush injury. The microRNA-301a knockout also led to significantly inhibited Wallerian degeneration in an in vivo sciatic nerve-transection model and in an in vitro nerve explant block model. Schwann cells with the microRNA-301a knockout showed inhibition of phagocytosis and migration, which was reversible under transfection with microRNA-301a mimics. Rescue experiments involving transfection of microRNA-301a-knockout Schwann cells with microRNA-301a mimics or treatment with the C–X–C motif receptor 4 inhibitor WZ811 indicated the mechanistic involvement of the Yin Yang 1/C–X–C motif receptor 4 pathway in the role of microRNA-301a. Combined with our previous findings in macrophages, we conclude that microRNA-301a plays a key role in peripheral nerve injury and repair by regulating the migratory and phagocytic capabilities of Schwann cells and macrophages via the Yin Yang 1/C–X–C motif receptor 4 pathway.
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Dermatologica Sinica
·
2025
·
IF 2.2
Peng Yan,He Ling,Yang Xiaoqiong,Zhao Aijie
Background: Androgenetic alopecia (AGA), a kind of nonscarring baldness, is an androgen-dependent autosomal dominant polymorphism. Compound glycyrrhizin (CG), a compound preparation-containing glycyrrhizin, aminoacetic, and methionine, has been used to treat alopecia areata. Objectives: Our study aimed to explore the role of CG in hair regrowth in testosterone-induced AGA mouse model and the proliferation of human hair dermal papilla cells (HDPCs) and the related molecular mechanisms. Methods: The AGA mouse model was established through the injection of testosterone. The in vivo therapeutic effects of CG on AGA were assessed by detecting skin color changes, hair growth time, average hair length, and histological score. Human HDPCs after CG treatment were subjected to CCK-8 assay to evaluate cell proliferation. The impacts of CG on growth factor expression and the Wnt/β-catenin signaling in mouse dorsal skins and HDPCs were determined by undertaking western blotting, reverse transcription-quantitative polymerase chain reaction, and immunofluorescence staining. The Wnt/β-catenin pathway inhibitor XAV939 was used to validate the beneficial role of Wnt/β-catenin pathway activation in CG-mediated hair growth. Results: Our results showed that CG facilitated new hair growth and increased hair follicle numbers on the depilated dorsal area of the model mice. Furthermore, CG administration upregulated p-GSK-3β, β-catenin, cyclin D1, Axin2, LEF1, fibronectin, and survivin levels in the dorsal skins of model mice. In vitro assays revealed that CG promoted cell proliferation, elevated the mRNA levels of growth factors-related genes (insulin-like growth factors-1 (IGF-1), fibroblast growth factor-2 (FGF-2), and hepatocyte growth factor (HGF)), and increased p-GSK-3β, β-catenin, cyclin D1, Axin2, LEF1, fibronectin, and survivin protein levels in HDPCs. Importantly, XAV939 treatment debilitated the promotion of CG on hair regrowth in model mice and human hair dermal papilla cell (HPDC) proliferation. Conclusion: The beneficial role of CG in hair regrowth in AGA mice and HDPC cell proliferation depends on the Wnt/β-catenin pathway activation and the subsequent growth factor production in hair follicles. These results imply the potential of CG in the treatment of AGA.
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BIOTECHNOLOGY LETTERS
·
2023
·Vol. 45
·期 10
·1355-1364
·
IF 2.7
Gao Yahui,Li Fen,Wang Yulu,Chen Zhou,Li Zijie
Purpose In our previous study, we constructed a one-pot multi-enzyme system for rare ketoses synthesis based on L-rhamnulose-1-phosphate aldolase (RhaD) from accessible glycerol in vitro. To eliminate tedious purification of enzymes, a facile Escherichia coli whole-cell cascade platform was established in this study. Methods To enhance the conversion rate, the reaction conditions, substrate concentrations and expressions of related enzymes were extensively optimized. Results The biosynthetic route for the cascade synthesis of rare ketoses in whole cells was successfully constructed and three rare ketoses including D-allulose, D-sorbose and L-fructose were produced using glycerol and D/L-glyceraldehyde (GA). Under optimized conditions, the conversion rates of rare ketoses were 85.0% and 93.0% using D-GA and L-GA as the receptor, respectively. Furthermore, alditol oxidase (AldO) was introduced to the whole-cell system to generate D-GA from glycerol, and the total production yield of D-sorbose and D-allulose was 8.2 g l −1 only from the sole carbon source glycerol. Conclusion This study demonstrates a feasible and cost-efficient method for rare sugars synthesis and can also be applied to the green synthesis of other value-added chemicals from glycerol.
研究领域:
生物化学
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合成生物学
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代谢工程
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POLYMER SCIENCE SERIES B
·
2015
·Vol. 57
·期 5
·530-537
·
IF 0.577
Ban Yingying,Wang Youfa
A kind of novel bioinspired polymeric material based on poly( D,L -lactic acid) was prepared. Firstly, the maleic anhydride (MA) grafted to PDLLA (PDLLA- g -MA) was synthesized, then PDLLA- g -MA reacted with Gly-Arg-Gly-Asp-Tyr (GRGDY) pentapeptide, which can give the biocompatibility and bioactivity to polymer. Then nano-hydroxyapatite (HAP) was added to enhance the hydrophilicity and cell adhesion of composite obtained. According to spectroscopy (FTIR, 13 C NMR) and pyrolysis-gas chromatography-mass spectrometry, PDLLA- g -MA and PDLLA- g -MA- g -GRGDY were synthesized successfully. Static water contact angle test, thermogravimetry, methylthiazolyl tetrazolium assay and scanning electron microscopy were used to characterize the properties of materials. The results show that the new composite material had better hydrophilicity and cell affinity than pure PDLLA, so it can be used as a potential composite membrane for tissue engineering application.
研究领域:
生物材料
·
组织工程
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高影响因子
Journal of Physical Chemistry C
·
2024
·
IF 3.3
Zhi-Qiang Wang,Wanjun Guo,Shengchao Jia,Yaxuan Jing,Shiting Li,Yong Guo,Xiaohui Liu,Xue-Qing Gong,Yanqin Wang
The selective production of primary amines through reductive amination has received increasing attention due to their extensive applications, while efficient reductive amination is still challenging. Herein, a novel strategy was developed to realize the efficient reductive amination of carbonyl compounds with NH3 via d–d orbital coupling strategies in RuCo alloys. The designed series of RuCo alloy catalysts not only exhibited excellent performance in conversion of various substrates but also demonstrated universal applicability across different supports. The key role of RuCo alloy is its unique ability to effectively catalyze the ammonolysis of a Schiff base, the rate-determining step in reductive amination. Theoretical studies reveal that the d–d orbital coupling strategies break the five d orbital equivalent contributions of metal (Co or Ru) atoms, and the d orbitals primarily contribute through the dz2 and dx2-y2 orbitals of Ru and the dz2 orbital of Co on the RuCo(001) surface, which significantly driving the homolytic NH3 dissociation during the ammonolysis of the Schiff base. Furthermore, stable Co–H covalent bond species and RuCo-NH2 half-ionic and half-covalent bond species on the RuCo(001) surface were generated with lower energy levels. This work not only achieves efficient reductive amination but also provides an atomic orbital-level understanding of the d–d orbital coupling strategies for heterogeneous catalysis.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2024
·
IF 20.2
Ruichen Geng,Ranran Wei,Xuewen Wei,Xiaoying Zhang,Yaxuan Li,Zekai Shen,Yinglong Wang,Liang Wang,Shuli Yin
Designing highly active and selective electrocatalytic catalysts to accelerate 5-hydroxymethylfurfural (HMF) oxidation is significant but challenging in biomass upgrading. Herein, the high-entropy alloy AuCuAgPdNi nanowires catalyst with a self-complementary effect via a simple one-pot wet chemical method is reported. Notably, AuCuAgPdNi NWs show excellent activity and selectivity during HMF oxidation with a 2,5-furandicarboxylic acid (FDCA) yield of 95.5 % and a selectivity of 98.4 % at the potential of 1.60 V vs. RHE. Moreover, a flow electrolytic cell is designed to couple the high-performance HMF oxidation reaction (HMFOR) at the anode and the faradaic efficiency of the hydrogen evolution reaction approaching 100 % at the cathode. In-situ Fourier transform infrared (in-situ FTIR) results reveal the relationship between the HMFOR pathway and potential. Theoretical calculation shows that AuCuAgPdNi NWs promote the exchangeability of surface electrons through the self-complementary effect generated by d - d orbital coupling, thus achieving efficient HMFOR. This work provides insights into the innovative synthesis and mechanisms inherent catalytic of high-entropy alloy systems and offers ideas for the value-added conversion of biomass.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2025
·
IF 3.6
Jin Li-Qun,Liu Meng-Dan,Guan Zi-Yu,Li Yi-Xin,Xue Ya-Ping,Liu Zhi-Qiang,Zheng Yu-Guo
DAAO is applied as a potential catalyst in the biosynthesis of L-PPT. However, its low solubility expression constrains its broader industrial application. Herein, a novel DAAO derived from Cladophialophora carrionii ( Cc DAAO) was identified, which demonstrated superior catalytic performance toward D-Ala (specific activity: 106.38 ± 1.21 U/mg, K m : 1.56 ± 0.06 mM), along with remarkable thermostability and broad substrate spectrum. Under optimal culture conditions, the soluble expression level of Cc DAAO was enhanced through a co-expression strategy with molecular chaperones, and the enzyme activity increased by 36.3% compared with the initial level. Subsequently, Cc DAAO was constructed as a fusion protein (CGD) with catalase from Geobacillus sp. CHB1 ( Gb CAT) and applied in a D-amino acid aminotransferase (DAAT)-mediated cascade system. In a 2 L reaction system, this cascade system achieved complete conversion (> 99%) of 1 M D,L-PPT within 8 h, exhibiting a yield of 11.26 g/L/h for PPO, which represents a significant improvement over existing reports. This study presents a promising practical approach for the industrial production of optically pure L-PPT.
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IRANIAN POLYMER JOURNAL
·
2016
·Vol. 25
·期 5
·443-454
·
IF 1.684
Kong Lingqian,Ju Xiuping,Zhang Yan,Zhao Jinsheng,Xu Jianzhong
Abstract Two new donor–acceptor–donor (D–A–D) type monomers, 2-dodecyl-4,7-bis(4-methoxythiophen-2-yl)-2 H -benzo[ d ] [ 1 – 3 ] triazole (MOTBT) and 2-dodecyl-4,7-bis(4-methylthiophen-2-yl)-2 H -benzo[ d ] [ 1 – 3 ] triazole (MTBT) were synthesized by Stille coupling and the corresponding polymers, PMOTBT and PMTBT were obtained by electropolymerization method. The properties of the monomers and the polymers were studied by cyclic voltammetry (CV), ultraviolet–visible–near-infrared (UV–Vis–NIR) and spectroelectrochemistry. The CV results demonstrated ambipolar processes (n- and p-doping) for both polymers. According to the spectroelectrochemistry tests, the polymer PMOTBT revealed a saturated blue color in the reduced state and a transparent light blue color in the oxidized state, and the PMTBT polymer could switch between neutral orange color and oxidized light bluish-green color in the redox process. Furthermore, in the visible region, the optical contrasts were 51 % (at 645 nm) and 34 % (at 430 nm) for PMOTBT and PMTBT, respectively, with the switching times of 0.29 and 1.2 s from reduced state to oxidized state. In the NIR region, higher optical contrast ratios of 76 % (at 1600 nm for PMOTBT) and 67 % (at 1500 nm for PMTBT) as well as faster response times (0.90 and 0.35 s) were obtained. We also found that both polymers had the relative high coloration efficiency as much as 372 and 312 cm 2 /C and a satisfactory stability. Therefore, both polymers are promising candidates in displays and opto-electronic devices as electrochromic materials. Graphical abstract
研究领域:
材料科学
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电化学
·
光学
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ECS Journal of Solid State Science and Technology
·
2025
·Vol. 14
·期 11
·111007
·
IF 2.2
Shanxin Xiong,Huanyu Tan,Jiaxue Hou,Min Chen,Juan Wu,Yukun Zhang,Xicheng Lu,Yongxin Wang,Xiaoqin Wang,Chunxia Hua,Jia Chu,Ming Gong
Covalent organic frameworks (COFs) have attracted extensive interest in the field of optoelectronic functional materials due to their high design freedom, flexibility in structural modulation, and ease of introducing functional groups. Pyrene, the smallest densely-packed polycyclic aromatic hydrocarbon, possesses excellent thermal stability and high carrier mobility properties. As a building block of COFs, its integration has attracted wide attention. Here, 1,3,6,8-tetrakis(4-aminophenyl)pyrene (Py(NH2)4) and 1,3,6,8-tetrakis((4-(4′-amino-1-ethynylphenyl)pyrene (TAEPy) were selected as amine construction units. Also, 1,3,6,8-tetrakis(4-formylphenyl)pyrene (Py(CHO)4) and 1,3,6,8-tetrakis((4-(4′-formyl-1-ethynylphenyl)pyrene (TFEPy) were used as aldehyde building units. We synthesized two imine-based COF electrochromic materials (Py-Py COF and TATFEPy COF) via a solvothermal method. The acetylene group, with its high electron mobility and electron density, acts as a π bridge. Together with the pyrene group as the donor (D), it forms a D-π-D-π structure. This structure enhances π–π stacking between COF molecules and boosts material stability. Tests show that TATFEPy COF outperforms Py-Py COF in electrochromism. Their contrasts are 0.45 and 0.30, and the coloring/bleaching times are 8.5/12.0 s and 12.0/20.0 s, respectively. After 2000 s cycling, contrast retention is 73.3% for TATFEPy COF and 54.0% for Py-Py COF. Therefore, constructing D-π-D-π molecular structures is an effective method for designing and synthesizing high-performance electrochromic polymers, enhancing their optoelectronic properties.
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Fermentation-Basel
·
2025
·Vol. 11
·期 12
·667
·
IF 3.3
Yutong Lu,Huayang Tang,Dexun Fan,Qingzhu Wang,Shuangyan Han
D-Allulose is a promising low-calorie rare sugar with significant health benefits. However, its industrial production is hindered by the low catalytic efficiency (≤33% conversion) and unfavorable equilibrium of the key enzyme, D-allulose 3-epimerase (DAE). To overcome this thermodynamic bottleneck, an in vitro synthetic enzymatic cascade based on a phosphorylation–dephosphorylation strategy was constructed. This engineered system comprises four synergistically operating enzymes: D-allulose-3-epimerase (DAE), L-rhamnulose kinase (RhaB), polyphosphate kinase (PPK), and acid phosphatase (AP). Through rational design and systematic optimization, the cascade achieved an exceptional 84.5% conversion yield from 50 mM D-fructose. Importantly, the system also maintained high conversion rates of 64.4% and 61.1% at high D-fructose loadings (50–100 g L−1). This performance, together with the integration of a low-cost PolyP6–PPK ATP regeneration module, underscores the potential industrial applicability of the proposed cascade strategy.
研究领域:
生物催化
·
酶工程
·
合成生物学
·
工业生物技术
·
碳水化合物化学
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ORGANIC ELECTRONICS
·
2019
·
IF 3.495
Fu-han Liu,Ju Bai,Guang Yu,Fang-hong Ma,Yan-jun Hou,Hai-jun Niu
A series of novel polyazomethines containing EDOT and thiophene units coded as PEDOTs (PEDOT-TPA, PEDOT-TDD, PEDOT-THIO, PEDOT-APM) and PTHIOs (PTHIO-TPA, PTHIO-TDD, PTHIO-THIO, PTHIO-APM) were synthesized successfully. They were gained in short time, low cost and mild reaction conditions by a straightforward synthetic path. It can be easily found from the electrochemical properties of these polymers that the polymers containing 3,4-ethylenedioxythiophene (EDOT) unit (PEDOTs) have lower initial oxidation potentials as compared to the polymers containing thiophene unit (PTHIOs). Spectroelectrochemical properties reveal that all of the polymer films exhibit outstanding multielectrochromic behaviors, distinct color contrast and excellent electrochemical stabilities. Kinetic studies show that the PEDOT-TPA film has the shortest response time (a color switching time of 4.54 s and a bleaching time of 3.88 s at 428 nm) and the PEDOT-THIO film exhibits the highest CE of 343.56 cm 2 C −1 . Typical I-V characteristics of the ITO/plymer/Al memory devices exhibit that all the memory devices based on PEDOTs and PTHIOs exhibited nonvolatile flash memory characteristics, with an ON/OFF current density ratio of 10 3 -10 4 and low threshold voltages (around 1 V). Thus, these characteristics illustrate that these novel polymers have great potential in optoelectronics applications as electrochromic materials and memory devices.
研究领域:
材料科学
·
电化学
·
光学
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高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2021
·
IF 3.493
Dong-Xu Jia,Chen-Yi Sun,Yi-Ting Jin,Zhi-Qiang Liu,Yu-Guo Zheng,Mian Li,Hong-Yan Wang,De-Shui Chen
Allulose is an epimer of d -fructose with multiple nutritional functions, which can be biologically synthesized from d -fructose employing d -allulose 3-epimerase (DAE). In this study, NCBI database mining approach was applied to screen novel DAEs, and Novibacillus thermophilus DAE (NtDAE) exhibiting a high activity of 273.9 U/(g⋅wet cell weight) was selected for investigation. NtDAE showed optimum reaction temperature and pH at 70 °C and 7.0, respectively. In the presence of Co 2+ , its activity was improved by 100.1 %, and the half-life ( t 1/2 ) values reached up to 846.3, 539.2 and 47.8 min at 40, 50 and 60 °C, respectively. Higher k cat / K m values toward d -allulose indicated that the preferred physiological substrate of NtDAE was d -allulose. The conversion yield of 500 g/L d -fructose to d -allulose at 60 °C achieved 29.7 %, and further enhanced to 51.6 % with adding 1.1 M borate. This study provided a useful guidance of further engineering and application of NtDAE for realization of industrial production of d -allulose.
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