ANALYTICAL BIOCHEMISTRY
·
2016
·
IF 2.243
Xiaoxia Shi,Shanshan Sha,Likun Liu,Xin Li,Yufang Ma
Mycobacterium tuberculosis dTDP- d -glucose 4,6-dehydratase (RmlB) is the second enzyme for the biosynthesis of dTDP- l -rhamnose, which is a sugar donor to the synthesis of the cell wall linker, d - N -acetylglucosamine- l -rhamnose. RmlB is essential to mycobacterial growth and is not found in humans; therefore, it is a potential target for developing new anti-tuberculosis drugs. So far, there has been no suitable method for high-throughput screening of RmlB inhibitors. Here, the recombinant M. tuberculosis RmlB was purified and an absorbance-based microtiter plate assay was developed for RmlB activity. It could be used for high-throughput screening of RmlB inhibitors. The kinetic properties of M. tuberculosis RmlB, including optimal pH, optimal temperature, the effect of metal ions , and the kinetic parameters , were determined with this assay. The inhibitory effects of dTTP and dTDP on M. tuberculosis RmlB were also studied with the assay.
研究领域:
微生物学
·
药物开发
·
生物化学
✓ 已验证
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Juexin Huang,Jingyi Xia,Sravan Baddi,Yu Zhao,Chuan-liang Feng
Piezoelectric biomaterials hold immense potential for next-generation microdevices, yet their piezoelectric performance often trails behind that of conventional synthetic counterparts. This study demonstrates a dual-scale polarization strategy to significantly enhance the piezoelectric response of d-phenylalanine derivatives (d-PheAD). Molecular-level polarization is amplified through ionic interactions with l-glutamic acid-modified Fe3O4 magnetic nanoparticles, while macroscopic alignment of d-PheAD nanoarrays under magnetic fields optimizes collective dipole orientation. This synergistic approach elevates the effective piezoelectric coefficient (deff) from 10.4 to 121.9 pm V–1, outperforming most biomaterial-based piezoelectric systems and many inorganic piezoelectric systems. Integrating these engineered assemblies into a triboelectric-enhanced piezoelectric nanogenerator yields unprecedented electrical outputs (2853 nA, 51.9 V), a 25-fold improvement over baseline configurations. The device achieves real-time detection of trace HCl gas at low concentrations, demonstrating superior sensitivity compared to commercial electrochemical sensors. By bridging molecular design and macroscopic engineering, this work establishes a scalable paradigm for deploying eco-friendly piezoelectric biomaterials in high-performance microelectronics and environmental monitoring technologies.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
传感器技术
·
环境监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Yang Liu,Kun Wang,Lei Jin,Yahan Li,Guanyu Chen,Hui Xu,Jie Chen,Guangyu He
Transition metal sulfides (TMSs) catalysts with high catalytic oxygen evolution reaction (OER) activity have been extensively studied, especially Fe and Co-based sulfides. Fe and Co active sites with a strong synergistic effect, which can adjust the electron density distribution and effectively improve the electrocatalytic OER activity. However, TMSs have poor stability in alkaline environment caused by metal ions and sulfur elements are facilitated to dissolve. In this work, TMSs was modified by polyaniline (PANI) to inhibit the precipitation of iron, cobalt, and sulfur elements and enhance its stability under alkaline conditions. Moreover, π-d structure can also be formed by the coating of PANI, which can further adjust its own electronic structure on the basis of stabilizing the TMSs structure, so as to improve the electrochemical performance, rendering them to stably operate at harsh environment for more than 90 h. These findings offer new guidance for improving the electrocatalytic stability of TMSs.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2018
·Vol. 8
·期 1
·1-10
·
IF 4.122
Chen Peng,Chen Fuchao,Zhou Benhong
Accumulating evidence has suggested that oxidative stress and apoptosis are involved in the ageing process. D-galactose (gal) has been reported to cause symptoms of ageing in rats, accompanied by liver and brain injuries. Our study aimed to investigate the potential antioxidative, anti-inflammatory and anti-apoptotic effects of ellagic acid and to explore how these effects act on rats in a D-gal-induced ageing model. Ageing was induced by subcutaneous injection of D-gal (100 mg/kg/d for 8 weeks). Ellagic acid was simultaneously administered to the D-gal-induced ageing rats once daily by intragastric gavage. Finally, the mental condition, body weight, organ index, levels of inflammatory cytokines, antioxidative enzymes, and liver function, as well as the expression of pro- and anti-apoptotic proteins, were monitored. Our results showed that ellagic acid could improve the mental condition, body weight, organ index and significantly decrease the levels of inflammatory cytokines, normalize the activities of antioxidative enzymes, and modulate the expression of apoptotic protein in ageing rats. In conclusion, the results of this study illustrate that ellagic acid was suitable for the treatment of some ageing-associated problems, such as oxidative stress, and had beneficial effects for age-associated diseases.
研究领域:
药理学
·
老年医学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 15
·3604
·
IF 4.2
Qiang Fu,Mingqiang Chen,Weixin Pang,Zengqi Liu,Zhen Xu,Xin Lei
The thermodynamic effect of octyl-β-D-glucopyranoside (OGP) on the formation of methane-1,3-dimethylcyclohexane (DMCH) hydrate was studied in this work. The thermodynamic equilibrium hydrate formation pressures between 275.15 K and 283.15 K were measured by the isothermal pressure search method. Different OGP aqueous solutions (0, 0.1, and 1 wt%) were used in this work. The experimental results show that OGP had no obvious thermodynamic inhibition on methane-DMCH hydrate formation when its concentration was low (0.1 wt%), whereas it had an inhibition on methane-DMCH hydrate formation when its concentration was high (1 wt%). The phase equilibrium hydrate formation pressure of the methane-DMCH-OGP system is about 0.1 MPa higher than that of the methane-DMCH system. The dissociation enthalpies of methane hydrate in different solutions remained uniform, which indicates that OGP was not involved in methane-DMCH hydrate formation. This phenomenon is explained from the perspective of the molecular structure of OGP. As a renewable and biological nonionic surfactant, the concentration of OGP in the liquid phase is low, so OGP can be added to the methane-DMCH system without significant thermodynamic inhibition.
研究领域:
化学工程
·
能源科学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2022
·
IF 5.911
Haiying Cui,Xiaojing Yang,Changzhu Li,Yong Ye,Xiaochen Chen,Lin Lin
Escherichia coli O157:H7 ( E. coli O157) is still one of the main pathogenic strains causing food poisoning. Here in, we established a strategy that can simultaneously remove free E. coli O157 and the biofilms it forms. Composite nanofiber (fP-NF) films were synthesized successfully by co-encapsulating E. coli O157 phages which isolated from domestic sewage and D -phenylalanine (D-Phe) into sodium alginate (SA)/polyethylene oxide (PEO) nanofibers by electrospinning. And dehydrated trehalose was added as a phage protectant. When SA:PEO is 3:9, the spinning solution has the optimum conductivity and viscosity, the average diameter of the obtained fP-NFs is 421.87 nm, and the mechanical strength and transparency of the fP-NF film are the best. The thermal stability of the fP-NF film was good, and the phage activity in the fiber was well preserved. The remaining population of phages in fP-NF membranes was still close to 10 6 PFU/mL after one week of placement, and the cumulative release efficiencies of phages at both room temperature (25 °C) and refrigerated storage temperature (4 °C) were above 70%. The antibacterial experiment showed that compared with the control group, the addition of D-Phe slightly enhanced the antibacterial activity of the nanofiber films against free E. coli O157, and the bactericidal rate within 8 h was 99.74%. On the other hand, D-Phe significantly enhanced the inhibitory activity of the nanofiber films against E. coli O157 biofilms, with the inhibition rate exceeding 99.99% within 72 h. The application results further confirmed that the addition of D-Phe could significantly enhance the inhibitory activity of nanofiber films on E. coli O157 proliferation and biofilm formation during short-term storage of beef and fruits and vegetables. It provides an important strategy and reference for the development of new active food packaging materials .
研究领域:
食品科学
·
微生物学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2022
·
IF 9.965
Qianqian Song,Junqi Li,Beiyi Zhang,Hu Li,Xiaoxu Liu
Despite the challenges on tuning the d-band structure of transition metals , the d-band is of great importance for promoting the interaction between catalytic and intermediates during the oxygen evolution reaction (OER) process. Herein, ultrafine Co nanoparticles embedded in the surface layer of nitrogen-doped carbon microspheres are prepared through an in-situ co-coordination strategy, and its d-band is modulated by introducing different Ni amounts. The introduction of Ni in the Co crystal lattice can tune the d-band center and unpaired electrons, which collectively result in an enhancement of OER activity and kinetics. By investigating the catalysts with Ni content from 0% to 75%, it is concluded that the catalyst with 25% Ni shows optimal OER activity, lower overpotential (285 mV at 10 mA cm −2 ) and higher current densities (73.75 mA cm −2 at 1.63 V). Moreover, the good stability is also demonstrated with the negligible decrease on current densities after 3000 CV cycles or 100 h of continuous test in alkaline media. This concept of modulating the d-band structure by introducing a transition metal with different contents in another transition metal crystal lattice could present an alternative pathway to the development of highly active catalytic materials for OER and beyond.
研究领域:
材料科学
·
电催化
✓ 已验证
DOI
高影响因子
International Journal of Nanomedicine
·
2016
·
IF 4.32
Jinyang Shen,Jianping Bi,Hongli Tian,Ye Jin,Yuan Wang,Xiaolin Yang,Zhonglin Yang,Junping Kou,Fei Li
Background Akebia saponin D (ASD) exerts various pharmacological activities but with poor oral bioavailability. In this study, a self-nanoemulsifying drug delivery system (SNEDDS) based on the drug–phospholipid complex technique was developed to improve the oral absorption of ASD.Methods ASD–phospholipid complex (APC) was prepared using a solvent-evaporation method and characterized by infrared spectroscopy, differential scanning calorimetry, morphology observation, and solubility test. Oil and cosurfactant were selected according to their ability to dissolve APC, while surfactant was chosen based on its emulsification efficiency in SNEDDS. Pseudoternary phase diagrams were constructed to determine the optimized APC-SNEDDS formulation, which was characterized by droplet size determination, zeta potential determination, and morphology observation. Robustness to dilution and thermodynamic stability of optimized formulation were also evaluated. Subsequently, pharmacokinetic parameters and oral bioavailability of ASD, APC, and APC-SNEDDS were investigated in rats.Results The liposolubility significantly increased 11.4-fold after formation of APC, which was verified by the solubility test in n-octanol. Peceol (Glyceryl monooleate [type 40]), Cremophor® EL (Polyoxyl 35 castor oil), and Transcutol HP (Diethylene glycol monoethyl ether) were selected as oil, surfactant, and cosurfactant, respectively. The optimal formulation was composed of Glyceryl monooleate (type 40), Polyoxyl 35 castor oil, Diethylene glycol monoethyl ether, and APC (1:4.5:4.5:1.74, w/w/w/w), which showed a particle size of 148.0±2.7 nm and a zeta potential of −13.7±0.92 mV after dilution with distilled water at a ratio of 1:100 (w/w) and good colloidal stability. Pharmacokinetic studies showed that APC-SNEDDS exhibited a significantly greater Cmax1 (733.4±203.8 ng/mL) than ASD (437.2±174.2 ng/mL), and a greater Cmax2 (985.8±366.6 ng/mL) than ASD (180.5±75.1 ng/mL) and APC (549.7±113.5 ng/mL). Compared with ASD, Tmax1 and Tmax2 were both remarkably shortened by APC-SNEDDS. The oral bioavailability in rats was enhanced significantly to 183.8% and 431.8% by APC and APC-SNEDDS, respectively.Conclusion These results indicated that APC-SNEDDS was a promising drug delivery system to enhance the oral bioavailability of ASD.
研究领域:
药剂学
·
药物代谢动力学
·
中药学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jialong Xu,Yiqing Chen,Shaoan Zhang,Hongqiang Zhu,Ziqing Mao,Ziyi Lin,Zhishan Chen,Huiwang Lian,Ling Gao,Yang Li
Phospholipase D (PLD) activity plays a critical role in tumor progression, yet its highly sensitive detection remains challenging. In this study, we developed metal halide perovskite nanoprobes via a dual-anchoring strategy that employed co-encapsulation of phospholipid and cholesteryl hemisuccinate (Chs) for the sensitive and selective monitoring of PLD activity. The Chs molecule not only enhances the stability of the phospholipid membrane via its cholesterol moiety but also passivates surface defects of the perovskite through the succinate group, endowing the nanoprobes with exceptional optical properties (PLQY >90 %) and long-term aqueous stability (> 7 days). The nanoprobe operates via a turn-off fluorescence response triggered by PLD-specific hydrolysis of the phospholipid layer, achieving a low detection limit of 0.51 U/L and a wide linear range (5–2000 U/L) with good anti-interference capability. Furthermore, the nanoprobes were successfully applied to in situ imaging of PLD activity in living cells, enabling discrimination between tumor cells with different PLD expression levels. This study not only paves the way for designing novel perovskite-based probes for biomedical applications but also offers a valuable fluorescent imaging tool to monitor tumor progression.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2025
·
IF 14.3
Yanhui Cao,Junhao Zeng,Xuerong Zheng,Yuan Liu,Junda Lu,Jinfeng Zhang,Yang Wang,Yida Deng,Wenbin Hu
Oxygen reduction reaction (ORR) in neutral electrolyte is urgently needed in various areas, such as metal-air batteries. However, the N-coordinated transition-metal single-atom electrocatalysts confront sluggish catalytic kinetics due to the inappropriate electronic structure and the as-resulted unreasonable adsorption strength towards oxygen-containing intermediates. In this work, we develop a strategy to tune the Fe d-orbital spin state by introducing inert Si atom into the first coordination sphere of Fe-N 4 moieties. The experimental and theoretical results suggest that Si atom generates the coordination field distortion of Fe and induces the Fe d-orbital spin state transforming from low to medium spin state. The optimized spin-electron filled state (t 2g 4 e g 1 ) of Fe sites weakens the adsorption strength to intermediates and reduces the energy barrier of *OH desorption. Consequently, Fe-Si/NC catalyst exhibits superior ORR performance compared with that of Fe-NC and commercial Pt/C, showing a more positive half-wave potential of 0.751 V (vs. RHE) in 0.1 mol/L phosphate buffered saline. In addition, Fe-Si/NC-based neutral zinc-air batteries show a maximum power density of 108.9 mW cm −2 and long-term stability for more than 200 h. This work represents the possibility of constructing distorted coordination configurations of single-atom catalysts to modulate electronic structure and enhance ORR activity in neutral electrolyte.
研究领域:
材料科学
·
电化学
·
催化科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Physical Chemistry C
·
2015
·
IF 4.772
Yongxin Tao,Xiaogang Gu,Linhong Deng,Yong Qin,Huaiguo Xue,Yong Kong
We report a Cu2+-modified β-cyclodextrin, Cu2-β-CD, that was self-assembled on poly(l-glutamic acid) (P-l-Glu) for chiral recognition of d-tryptophan (d-Trp). Cu2+ formed binuclear hydroxy-bridge (Cu(OH)2Cu) at the wider opening of the cavity of β-CD and acted as a cap to prevent high-energy water molecules from being released while forcing Trp isomers to enter through its narrower opening. Because H-bonds were favored to form between the high-energy water molecules and the amino groups of d-Trp inside the cavity of β-CD, d-Trp was thus recognized. The recognition capability of the Cu2-β-CD appeared to depend on the way the Cu2-β-CD self-assembled onto P-l-Glu, preferring via H-bonds rather than via electrostatic interactions, because electrostatic interactions between P-l-Glu and Cu(OH)2Cu could alter the configuration of the Cu2-β-CD cavity, resulting in weakened H-bonds between high-energy water molecules and d-Trp.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Fei Fan,Shiyu Han,Ying Li,Lu Qi,Jie Kang,Zhiping Chen,Chang Tian,DeChao Wang,Wenwu Zhou
The microenvironments including the surface hydroxyls , the oxygen vacancies , and the d-band center of hierarchical TS-1 zeolite pickled by hydrochloric acid were regulated by ZrO 2 , the effect of ZrO 2 on the physicochemical properties and the catalytic performances of the corresponding NiMoS supported catalysts were studied. The experimental and theoretical calculation results show that the d-band center, the surface hydroxyl groups , and the oxygen vacancies were regulated after incorporation of ZrO 2 . Resulting in the modulated acidity of the support and the metal support interaction of the catalysts, further promoting the formation of type-II NiMoS active phases. The catalytic performance evaluations show that catalyst NiMo/1-ZAT exhibits the superior HDS performance, kinetic study further demonstrated that the enhanced catalytic activity was mainly contributed by the improved direct desulfurization activity generated from the moderate support acidity, suitable MSI, highest sulfidation degrees, most type-II NiMoS active phase and highest activity of individual active sites.
研究领域:
催化化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）