CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2018
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IF 0.824
Lian-Ming ZHANG,Dong-You ZHANG,Ying ZENG,Jian-Ping LI
A novel strategy based on DNA induced conformation change was developed by rendering the imprinting cavity with refined structure in order to further improve the selectivity of molecular imprinting membrane. The double-stranded DNA (dsDNA) were first self-assembled on the surface of the gold electrode, then cimaterol (CIM) molecule, taken as the representative target, would bind with the groove of dsDNA to form complex template, together with which the molecular imprinting polymers (MIPs) was prepared by electropolymerization in the monomer solution. After the elution of CIM, the obtained MIPs sensor had a remarkably high selectivity toward CIM. The combination process of CIM and DNA was studied by UV-Vis spectroscopy and was found to follow the groove combination model. The optimal conditions of the system were optimized by electrochemical method. The results showed that the sensor had the best recognition ability toward CIM when the elution time was 35 min by ethanol-acetic acid (8:1, V/V ) as an eluent and rebound time was 45 min. This sensor exhibited a linear detection range of 1.0 × 10 −13 −1.0 × 10 −10 M with a detection limit of 3.2 × 10 −14 M. The sensor was successfully applied for detection of CIM in pork samples.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
Biointerphases
·
2019
·Vol. 14
·期 6
·
IF 2.009
Cheng Fang,Ma Xiaochun,Feng Qiancheng,Wang Hanqi,Yin Meng,He Wei
The reliability and stability of array slides are a big concern for array vendors and end users. Herein, we report on a new type of array slide with high reactivity toward DNA probes and low side reaction. A one-step surface reaction via the Michael addition involved in preparing array slides was developed and characterized by x-ray photoelectron spectroscopy, contact angle, and fluorescence labeling. The effects of array fabrication and storage conditions, i.e., spotting solution p H, high humidity, and long-term storage on the reactivity of the slides were examined. The fabricated DNA arrays could realize good hybridization efficiency (38.2% for slides with 0.88 pmol/cm 2 ), low limit of detection (4 × 10 −14 M), as well as high mismatch selectivity.
研究领域:
分子生物学
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生物化学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2022
·
IF 8.008
Yajun Liu,Yufeng Pei,Jingjing Xu,Yuanlei Cheng,Qingyi Tong,Huijuan You
Quantitatively analyzing the binding topology and reactivity is essential for understanding the cytotoxic or tumorigenic activities of bulky DNA adducts formed by chemotherapeutic drugs or carcinogens. Biochemical methods require purification of DNA and discontinuous steps to digest or label the adducts and thus have difficulties in identifying the binding topology and are not suitable for detecting unstable adducts. Herein, we used a single-molecule stretching assay to characterize the number of intercalative adducts, the formation kinetics, and the mechanical properties of intercalative DNA adducts based on measuring adduct-induced DNA elongation. We analyzed various reactive conditions, including formaldehyde-mediated anthracycline–DNA adducts, UV light-catalyzed psoralen–DNA adducts, and liver S9 fraction-catalyzed aflatoxin B1–DNA adducts. We showed that adduct formation abilities are correlated with the noncovalent intercalation binding ability. External forces on double-stranded DNA increased the intercalation of ligands and can result in a 1.8- to 5.3-fold increase in DNA adduct formation.
研究领域:
生物化学
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分子生物学
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癌症研究
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毒理学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Chunyan Wang,Cuiyan Han,Xiaoxue Du,Weiwei Guo
In addition to their roles as revolutionary genome engineering tools, CRISPR-Cas systems are also highly promising candidates in the construction of biosensing systems and diagnostic devices, which have attracted significant attention recently. However, the CRISPR-Cas system cannot be directly applied in the sensing of non-nucleic acid targets, and the needs of synthesizing and storing different vulnerable guide RNA for different targets also increase the application and storage costs of relevant biosensing systems, and therefore restrict their widespread applications. To tackle these barriers, in this work, a versatile CRISPR-Cas12a-based biosensing platform was developed through the introduction of an enzyme-free and robust DNA reaction network, the entropy-driven dynamic DNA network. By programming the sequences of the system, the entropy-driven catalysis-based dynamic DNA network can respond to different types of targets, such as nucleic acids or proteins, and then activate the CRISPR-Cas12a to generate amplified signals. As a proof of concept, both nucleic acid targets (a DNA target with random sequence, T, and an RNA target, microRNA-21 (miR-21)) and a non-nucleic acid target (a protein target, thrombin) were chosen as model analytes to address the feasibility of the designed sensing platform, with detection limits at the pM level for the nucleic acid analytes (7.4 pM for the DNA target T and 25.5 pM for miR-21) and 0.4 nM for thrombin. In addition, the detection of miR-21 or thrombin in human serum samples further demonstrated the applicability of the proposed biosensing platform in real sample analysis.
研究领域:
生物技术
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分子生物学
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分析化学
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诊断设备
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DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 6
·1995-2002
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IF 4.019
Mei-Ling Gao,Fang He,Bin-Cheng Yin,Bang-Ce Ye
An increasing number of studies have found that circulating exosomes play a vital role in the occurrence and metastasis of cancer. Therefore, a direct, sensitive and specific method for detection of tumor exosomes will contribute to the diagnosis and prognosis of cancer. In this work, we take advantage of the facile adaptability of aptamers to design an exosome quantitative method, which converts an exosome capture event to nucleic acid detection. With the help of a hairpin DNA cascade reaction (HDCR) and easy accessibility of DNA dendrimer self-assembly, dual signal amplification was achieved. A CD63 aptamer linked via a DNA probe to magnetic beads acts as the capture component. In the presence of target exosomes, aptamers identify and combine with exosomes, releasing the DNA probe as a trigger to initiate the HDCR (the first signal amplification process) by opening hairpin DNA (HP1) bound to gold nanoparticles (AuNPs). Fluorescently-labeled DNA dendrimers concatenate with HP1 as the second signal amplification stage to increase the signal-to-noise ratio. Under the optimal conditions, our method achieved a good linear response for HepG2 cell-derived exosomes in a concentration range from 1.75 × 103 to 7.0 × 106 particles per μL with a detection limit of 1.16 × 103 particles per μL. It also shows a good performance for detection of exosomes in biological samples.
研究领域:
生物分子工程
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癌症诊断
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纳米技术
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jia Chen,Xuliang Ouyang,Chenxiao Yu,Juan Xiang
Low-mass soluble β-amyloid oligomers (LSAβO) are critical Alzheimer’s disease (AD) biomarkers with significant diagnostic and therapeutic potential. However, their application in early screening and detection is limited by the reliance on complex analytical instruments and procedures. To address this, we developed a visual sensing platform for LSAβO detection using a functionalized pNIPAM-DNA hydrogel. Exploiting the temperature-responsive nature of pNIPAM, the hydrogel selectively incorporates and enriches LSAβO from solution via temperature-induced expansion and contraction. LSAβO binding to aptamers on the hydrogel triggers the formation of G-quadruplex DNAzymes, which catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine to generate a visible colorimetric signal. The hydrogel’s small pore size further enhances selectivity by excluding larger oligomers during real sample analysis. This sensor exhibits a linear detection range of 0.1–7.5 nM for LSAβO and a detection limit of 50 pM. Combining LSAβO enrichment and size exclusion, this functionalized pNIPAM-DNA hydrogel platform provides a cost-effective, highly sensitive, selective, and high-throughput approach for preliminary LSAβO screening and detection.
研究领域:
生物化学
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医学诊断
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材料科学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2018
·
IF 8.173
Wei Zhang,Zhichao Dai,Xue Liu,Jimin Yang
A high-performance sensing platform based on poly-xanthurenic acid (PXA) film functionalized MoS 2 nanosheets was developed for electrochemical detection of circulating tumor DNA in peripheral blood. The MoS 2 nanosheets were obtained using a simple ultrasonic method from bulk MoS 2 . The physical adsorption between MoS 2 and aromatic XA monomers effectively improved the electropolymerization efficiency, accompanied with an increased electrochemical response of PXA. The obtained PXA/MoS 2 nanocomposite not only served as a substrate for DNA immobilization but also reflected the electrochemical transduction originating from DNA immobilization and hybridization without any complex labelling processes or outer indicators. The immobilization of the probe ssDNA was achieved via noncovalent assembly due to the π–π interaction between PXA and DNA bases. After the hybridization of the probe ssDNA with the target DNA, the formation of helix structure induced the resulted dsDNA to be released from the surface of the PXA/MoS 2 nanocomposite. The detection limit of this constructed DNA biosensor was calculated in the linear target DNA concentrations range from 1.0 × 10 –16 mol/L to 1.0 × 10 -10 mol/L and it was found to be 1.8 × 10 –17 mol/L.
研究领域:
生物医学工程
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分子诊断学
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纳米生物学
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DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Chuiyu Zhu,Xiao Tian,Qiuyan Li,Zichang Dai,Lina Wang,Huamin Liu,Chenghong Li,Kashif Rafiq Zahid,Chunmei Wu,Hui Huang,Lichao Fang,Qiyuan Huang,Feixue Liu,Jun Deng,Yan Li,Tao Zeng,Junsong Zheng
Altered DNA methylation in the form of 5-methylcytosine (5-mC) patterns is correlated with disease diagnosis, prognosis, and treatment response. Therefore, accurate analysis of 5-mC is of great significance for the diagnosis of diseases. Here, an efficient enhanced photoelectrochemical (PEC) biosensor was designed for the quantitative analysis of DNA 5-mC based on a cascaded energy level aligned co-sensitization strategy coupling with the bridged DNA nanoprobe (BDN). Firstly, Au nanoparticle/graphite phase carbon nitride/titanium dioxide (AuNPs/g-C 3 N 4 @TiO 2 ) nanocomposite was synthesized through in situ growth of AuNPs on g-C 3 N 4 @TiO 2 surface as a matrix to provide a stable background signal. Next, BDN with a high mass transfer rate synthesized from a pair of DNA tetrahedral as nanomechanical handles was used as a capture probe to bind to the target sequence. The polydopamine nanosphere was applied to load with CdTe QDs (PDANS-CdTe QDs) as a photocurrent label of 5-mC antibodies. When the 5-mC existed, a large number of PDANS-Ab-CdTe QDs were introduced to the electrode surface, the formed CdTe QDs/AuNPs/g-C 3 N 4 @TiO 2 co-sensitive structure could effectively enhance the electron transfer capability and photocurrent response rate due to the effective cascade energy level arrangement, leading to a significantly enhanced photocurrent signal. The proposed PEC biosensor manifested a wide range from 10 −17 M to 10 −7 M and a detection limit of 2.2 aM. Meanwhile, the excellent performance indicated the practicability of the designed strategy, thus being capable of the clinical diagnosis of 5-mC.
研究领域:
生物医学工程
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分析化学
·
分子生物学
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DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Li Zou,Tingting Li,Ruidi Shen,Shaozhi Ren,Liansheng Ling
A label-free light-up fluorescent sensing strategy using hybridization chain reaction (HCR) amplification and DNA triplex assembly has been developed. Remarkably, the proposed fluorescence assay is successfully applied to the determination of avian influenza A (H7N9) virus DNA and thrombin. Herein, in the presence of targets, the target DNA/initiator triggers a cascade of hybridization events between H1 and H2 that yields nicked double helices analogous to alternating copolymers . With the additions of triplex-forming oligonucleotide (TFO) and berberine , the triplex structures form between HCR products and TFO. Then, a large amount of berberine can bind to the triplex structures and the sensing system exhibits a dramatic increase in the fluorescence intensity at 530 nm. Under optimal conditions, the label-free fluorescent sensing platform shows sensitive responses to H7N9 virus DNA and thrombin in the range of 0.2–100 nM and 0.5–200 nM, respectively. The detection limits of H7N9 virus DNA and thrombin are as low as 0.14 nM and 0.32 nM, respectively. Owing to the simplicity and low cost, the proposed strategy can be used in other biomarkers assays, providing a promising tool for clinical diagnosis and biomedical detection.
研究领域:
生物化学
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分子生物学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Li Zhang,Xiaofen Xiao,Yuzhi Xu,Danping Chen,Jun Chen,Yingjun Ma,Zong Dai,Xiaoyong Zou
A simple electrochemical strategy is reported for continuous monitoring of dynamic DNA methylation process over time. An electrochemical sensor was prepared by co-assembling of DNA probe and 6-mercapto-1-hexanol onto a gold electrode. The top of the DNA probe was labeled with 6-ferrocenylhexanethiol modified gold nanoparticle . The charge density between the C•G base pair was verified to be slightly reduced by DNA methylation, and could be further decelerated by ~ 25% upon co-locating a Br group onto methylated cytosine (mC). Therefore, in the presence of NaIO 4 /LiBr, the progressively methylated DNA on the sensor showed a clearly decreasing current over methylation time. The dynamic DNA methylation process was indicated continuously from the current decrease ratio, with a limit of detection of 0.0372 µM. The strategy is convenient, cost-effective, and enable continuous profiling methylation process without distortion. Besides, the strategy was successfully applied for the studies on inhibitor screening and flanking sequence preference of DNA methyltransferase 3a. The results show that the activity of DNA methyltransferase 3a can be mildly inhibited by epigallocatechin gallate , and varies towards different flanking sequence with an order of 5′-CCGG-3′ < 5′-CGCG-3′ < 5′-CGCA-3′.
研究领域:
电化学
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分子生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2013
·
IF 0.769
Yuan-Yuan JIANG,Kun WANG,Chong-Zheng XU,Xiao-Di YANG,Hui-Hui LI
In this work, the glassy carbon electrode modified by alizarin (AR) hybridized with graphene (CRG) and chitosan (CS) was prepared. The AR/CRG-CS electrode exhibited a pair of well-defined reversible redox peaks in 0.1 M phosphate buffer solution (PBS, pH 7.4) with the oxidation peak at −0.573 V and the reduction peak at −0.652 V. It exhibited a two-proton and two-electron redox process and a fast heterogeneous electron transfer rate with the rate constant ( K s ) of 1.69 s −1 using the cyclic voltammetry. Based on the characteristics of alizarin with the decreased current and negative potential shift after binding with human telomere DNA, a sensitive, simple, reliable and stable electrochemical method for the detection of human telomere DNA using the AR/CRG-CS electrode was developed. The electrochemical response showed a linear relationship with DNA concentration in the range of 8.0 × 10 −8 −1.4 × 10 −5 M ( R = 0.9995) with the linear regression equation as Δ I pc (μA) = 8.4860 + 0.5366 C (μmol) and the detection limit ( S/N ) as 2.0 × 10 −8 M.
研究领域:
生物化学
·
材料科学
·
分析化学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Wenjiao Zhou,Xue Gong,Yun Xiang,Ruo Yuan,Yaqin Chai
Visual detections have attracted great research attentions recently due to their convenient monitoring of the target analytes without using any advanced instruments. However, achieving visual detection of trace amounts of biomolecules with PCR-like sensitivity remains a major challenge. In current work, we describe a new quadratic signal amplification strategy for sensitive visual detection of HIV DNA biomarkers based on exonuclease III (Exo III)-assisted DNA recycling amplification and DNAzymes. The presence of the target HIV DNA leads to two independent and simultaneous DNA recycling processes to achieve quadratic signal amplification with the assistance of Exo III. This quadratic signal amplification results in catalytic cleavage of the G-quadruplex sequence-locked hairpin probes to release numerous active G-quadruplex sequences, which further associate with hemin to form DNAzymes and cause significantly intensified color change for sensitive and visual detection of HIV DNA down to 2.5 pM. The proposed visual detection method employs un-modified hairpin DNA as probes, avoids using any complex and expensive instruments for signal transduction and is essentially simple. This method also shows single-base mismatch discrimination capability as well. All these features make our developed DNA detection method holds great potential for visual monitoring of various DNA biomarkers at ultralow levels with careful and proper probe designs.
研究领域:
分子生物学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
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