INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Jie Liu,Yiwei Wang,Yanchun Liu,Shuaiqi Shao,Xuejing Zheng,Keyong Tang
Soluble soybean polysaccharide (SSPS)-based composite films with the addition of nano zinc oxide (nZnO, 5 wt% based on SSPS) and tea tree essential oil (TTEO, 10 wt% based on SSPS) were developed by the casting method. The effect of the combination of nZnO and TTEO on the microstructure and physical, mechanical and functional properties of SSPS films was evaluated. The results showed that the SSPS/TTEO/nZnO film exhibited enhanced water vapor barrier properties, thermal stability , water resistance, surface wettability, and total color difference, and almost completely prevented ultraviolet light transmission. The addition of TTEO and nZnO had no significant effect on the tensile strength and elongation at break of the films, but decreased the percentage of light transmittance of the films at 600 nm from 85.5 % to 10.1 %. The DPPH radical scavenging activity of the films significantly increased from 46.8 % (SSPS) to 67.7 % (SSPS/TTEO/nZnO) due to the presence of TTEO. Scanning electron microscopy analysis indicated that nZnO and TTEO were evenly dispersed in the SSPS matrix. The synergistic effect of nZnO and TTEO endowed the SSPS film with excellent antibacterial activity against E. coli and S. aureus , suggesting that the SSPS/TTEO/nZnO film could be a promising material for active packaging applications.
研究领域:
材料科学
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食品科学
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纳米技术
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高分子科学
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产品关联
DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2023
·
IF 2.9
Yuan Meng,Cao Yu,Zheng Haoyang,Chen Kunlin,Lu Yuping,Wang Jing,Zhu Liqin,Chen Ming,Cai Zhipeng,Shen Yonggen
This study used glucose, fructose, maltose and dextran to explore the effects of different carbohydrates on the Maillard reaction of casein phosphopeptides (CPP). The color parameter results showed that heating time from 1 to 5 h led to brown color, which was consistent with the observed increased in browning intensity. Fourier transform infrared spectroscopy results verified that four carbohydrates reacted with CPP to produce Maillard conjugates. Fluorescence spectroscopy showed that the Maillard reaction changed the tertiary structure of CPP by decreasing the intrinsic fluorescence intensity and surface hydrophobicity compared with the CPP-carbohydrate mixture. At the same time, the Maillard reaction effectively improved the emulsifying properties, reducing power and DPPH radical scavenging activity of CPP. Furthermore, this study also found that glucose and fructose improved CPP more than maltose and dextran. Therefore, monosaccharides have good potential in modifying CPP via the Maillard reaction. Graphical Abstract
研究领域:
食品科学
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化学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
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IF 9.8
Jiao Wu,Xiang-Tan Xu,Cheng Xing,Xin-Bao Hao,Xing-Yue Fang,Zhi-Hao Xie,Shan Zhao,Jing-Lin Gao,Lu Xu,Shi-Jie Wang
Honey has been recognized for its role in disease prevention through nutritional modulation. In this study, we comprehensively assessed the physicochemical parameters, metabolic profile, antioxidant and anti-inflammatory activities of multifloral honeys produced by Apis cerana cerana in Sansha City (SS), Hainan Province. Metabolomic analysis identified SS honey had multiple bioactive compounds known for antioxidant and anti-inflammatory properties. Compared to A. cerana cerana honey from central regions of Hainan Province, SS honey showed higher levels of TFC (113.80 mg RE/100 g) and TPC (45.86 mg GAE/100 g). Furthermore, SS honey demonstrated greater antioxidant activity, as evidenced by FRAP (1503.16 μmol TE/kg), DPPH IC 50 (36.13 mg/mL), and ABTS IC 50 (11.03 mg/mL). SS honey significantly reduced the levels of cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), and tumor necrosis factor-α (TNF-α) in lipopolysaccharide (LPS)-induced RAW 264.7 cells, along with suppressing the mRNA expression of these inflammatory markers.
研究领域:
养蜂学
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生物化学
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药理学
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营养科学
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代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2023
·
IF 6.7
Youzhi Wang,Huifang Wang,Guoqing Yang,Qingjing Hao,Kan Yang,Huizhen Shen,Yulong Wang,Jinxin Wang
It is well known that chronic obstructive pulmonary disease (COPD) patients are always trapped in the vicious circle of inflammation and oxidative stress , therefore anti-inflammatory and antioxidant bifunctional agents may interrupt this vicious cycle in COPD. Phosphodiesterase 4 (PDE4) inhibitors, as anti-inflammatory drugs, have been used for COPD treatment in clinical, and the PDE4 inhibitors with antioxidant properties may be a good strategy to design bifunctional agents for COPD. Sappanone A was the first PDE4 inhibitor with antioxidant properties we identified from natural products in our previous study, which was used by us as a hit compound to design new bifunctional agents for COPD in this study. 27 derivatives of sappanone A including homoisoflavonoids , aurones and chalcones were designed and synthesized by innovatively fusing the antioxidant pharmacophore of catechol from polyphenols and the pharmacophore of catechol ether abstracted from the PDE4 inhibitors of the catechol ether class such as rolipram , roflumilast and apremilast respectively. All the compounds were assayed for the PDE4 inhibitory and radical scavenging against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) activities in vitro. Herein we obtained a series of bifunctional compounds with better PDE4 inhibitory activity than sappanone A, and their free radical scavenging activities were superior to edaravone in vitro. In addition, they can reduce tumour necrosis factor-alpha (TNF-α) production induced by lipopolysaccharide (LPS) in RAW264.7 macrophages and malondialdehyde (MDA) production induced by Fe 2+ in mouse lung homogenate . Meanwhile, it showed outstanding abilities in reducing Fe 3+ and complexing Fe 2+ . 6o , as the candidate anti-inflammatory and antioxidant bifunctional compound, exhibited good drug-likeness, ADME (Absorption, Distribution, Metabolism, Excretion) properties and human liver microsomal stability. In vivo, 6o (50 mg/kg and 100 mg/kg, i. p.) distinctly prevented LPS-induced serum levels of TNF-α in mice. In conclusion, the preliminary investigation provided a novel class of PDE4 inhibitors with antioxidant properties as bifunctional agents for the potential treatment of COPD, which can interrupt the vicious cycle of chronic inflammation and oxidative stress in COPD.
研究领域:
药理学
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药物化学
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呼吸医学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2024
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IF 4.4
Yajie Luan,Huaqi Wang,Changfeng Han,Xiuying Zhao,Youping Wu
The aging degradation process is significantly accelerated in unsaturated natural rubbers (NR) due to their susceptibility to α-H bond dissociation caused by exposure to heat and oxygen. Recently, the European Union has listed widely used antioxidant N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) as the restricted category of compounds, sparking a growing desire to find environmentally friendly alternatives. Through a combination of molecular simulation and experimental methods, this study aims to investigate the protection mechanism and conformational relationship of the natural antioxidant gallic acid (GA) and its ester derivatives within the NR matrix. The ultimate objective is to identify natural antioxidants that possess the capability to effectively substitute 6PPD. Simulation results of the chemical aging factor suggest that the phenolic hydroxyl hydrogen dissociation energy of GA is lower than that of the α-H bond of NR, thereby delaying the aging process. Based on the influence of physical aging factors, the polarity of GA derivatives decreases as the modified alkyl chain lengthens, improving the compatibility and migration resistance with NR, strengthening interaction force, and lowering oxygen permeability. Additionally, comprehensive simulation results indicate that octyl gallate derivatives exhibit notable antioxidant properties. Subsequent DPPH radical scavenging experiments, microstructural characterizations, and macroscopic mechanical property tests have confirmed the optimal antiaging properties of octyl gallate in NR, which these ester derivatives are expected to be a nontoxic, nonhazardous, and safe natural antioxidant alternative to 6PPD.
研究领域:
材料科学
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高分子化学
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环境科学
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化工工程
✓ 已验证
产品关联
DOI
高影响因子
Microorganisms
·
2024
·Vol. 12
·期 11
·2229
·
IF 4.1
Xiqian Tan,Bingyu Ma,Xiaoqing Wang,Fangchao Cui,Xuepeng Li,Jianrong Li
Exopolysaccharides (EPSs) secreted by lactic acid bacteria have the potential to enhance human health by showing various biological functions. This study investigated the biological role and antibiofilm properties of EPS715, a new neutral EPS produced by pickled vegetables originating fromLactobacillus plantarumPC715. The results indicate that EPS715 is primarily composed of rhamnose, glucose, and mannose. Its molecular weight (Mw) is 47.87 kDa, containing an α-glucoside linkage and an α-pyranose ring. It showed an amorphous morphology without a triple helix structure. Furthermore, EPS715 showed improved antioxidant activity. Specifically, its scavenging capacity of ABTS+radicals, DPPH radicals, and the hydroxyl (·OH) reduction capacity at 5 mg/mL was 98.64 ± 2.70%, 97.37 ± 0.79%, and 1.64 ± 0.05%, respectively. Its maximal scavenging capacity was >40%, and the hydroxyl (·OH) radical scavenging ability was dose-dependent. Moreover, the biofilm of various pathogens includingS. aureus,B. cereus,S. saprophyticus,Acinetobacterspp., andH. alveiwas substantially dispersed and affected by EPS715, with a maximum inhibition rate of 78.17% forH. alvei. The possible mechanism by which EPS715 shows antibiofilm properties against theH. alveimay be attributed to its effects on the auto-aggregation, hydrophilic characteristics, and motility ofHafniaspp. Thus, EPS715 has significant antioxidant and antibiofilm characteristics that may hold substantial potential for applications in food and medicinal products.
研究领域:
微生物学
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食品科学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 15
·10410-10415
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IF 3.9
Jingjing Zhao,Dou Qian,Luyang Zhang,Xiao Wang,Jianfeng Zhang
Epigallocatechin gallate (EGCG) has attracted increasing attention thanks to its multi-bioactivities, and people are keen on improving the antioxidative and antibacterial performance of EGCG. Based on the favorable biofunctionality of Zn2+ and chitosan (CS), an EGCG derivative with a novel formula, i.e., EGCG–Zn–CS, is presented in this study. The structure of EGCG–Zn–CS was characterized by FT-IR, UV-vis, TGA, XPS, and SEM–EDS. The radical elimination results indicate that 0.1 mg mL−1 of EGCG–Zn–CS demonstrates DPPH radical and hydroxyl radical scavenging activities of 94.8% and 92.3%, while 0.1 mg mL−1 of EGCG exhibits only 78.5% and 75.6%, respectively, which means improved antioxidative activity of EGCG–Zn–CS was obtained. Inhibitory experiments against Staphylococcus aureus and Escherichia coli reveal that the minimal inhibitory concentrations (MICs) of EGCG–Zn–CS were 15.625 μg mL−1 and 187.5 μg mL−1, whereas the minimal bactericide concentrations (MBCs) were 46.875 μg mL−1 and 750 μg mL−1, respectively, which indicate that EGCG–Zn–CS exerts much higher antibacterial activity than EGCG. It can be concluded that the complexing of zinc cations and CS could amazingly improve both the antioxidative and antibacterial activity of EGCG, and it is expected that an exploration of EGCG–Zn–CS may inspire the development of simultaneous effective antioxidant and antibacterial agents.
研究领域:
药学
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食品科学
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材料科学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2024
·
IF 9.4
Yongsheng Zheng,Dong Yang,Botao Gao,Shuai Huang,Yubo Tang,Qingde Wa,Yong Dong,Shan Yu,Jun Huang,Sheng Huang
Chronic diabetic wounds are a severe complication of diabetes, often leading to high treatment costs and high amputation rates. Numerous studies have revealed that nitric oxide (NO) therapy is a promising option because it favours wound revascularization. Here, base-paired injectable adhesive hydrogels (CAT) were prepared using adenine- and thymine-modified chitosan (CSA and CST). By further introducing S-nitrosoglutathione (GSNO) and binary L-arginine (bArg), we obtained a NO sustained-release hydrogel (CAT/bArg/GSON) that was more suitable for the treatment of chronic wounds. The results showed that the expression of HIF-1α and VEGF was upregulated in the CAT/bArg/GSON group, and improved blood vessel regeneration was observed, indicating an important role of NO. In addition, the research findings revealed that following treatment with the CAT/bArg/GSON hydrogel, the viability of Staphylococcus aureus and Escherichia coli decreased to 14 ± 2% and 6 ± 1%, respectively. Moreover, the wound microenvironment was improved, as evidenced by a 60 ± 1% clearance of DPPH. In particular, histological examination and immunohistochemical staining results showed that wounds treated with CAT/bArg/GSNO exhibited denser neovascularization, faster epithelial tissue regeneration, and thicker collagen deposition. Overall, this study proposes an effective strategy to prepare injectable hydrogel dressings with dual NO donors. The functionality of CAT/bArg/GSON has been thoroughly demonstrated in research on chronic wound vascular regeneration, indicating that CAT/bArg/GSON could be a potential option for promoting chronic wound healing. Statement of significance This article prepares a chitosan hydrogel utilizing the principle of complementary base pairing, which offers several advantages, including good adhesion, biocompatibility, and flow properties, making it a good material for wound dressings. Loaded GSNO and bArg can steadily release NO and L-arginine through the degradation of the gel. Then, the released L-arginine not only possesses antioxidant properties but can also continue to generate a small amount of NO under the action of NOS. This design achieves a sustained and stable supply of NO at the wound site, maximizing the angiogenesis-promoting and antibacterial effects of NO. More neovascularization and abundant collagen were observed in the regenerated tissues. This study provides an effective repair hydrogel material for diabetic wound.
研究领域:
生物医学工程
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材料科学
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药剂学
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伤口愈合
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2022
·
IF 5.318
Songqi Liu,Zaihan Chen,Huange Zhang,Yuanli Li,Taxipalati Maierhaba,Jianhui An,Zhi Zhou,Lingli Deng
To explore the dihydromyricetin or eugenol loaded nanofiber's potential as food active packaging, 0%, 0.1%, 0.5%, and 1.0% dihydromyricetin or eugenol loaded nanofibers were fabricated by electro-blowing spinning, which increased the yield of nanofibers by a factor of 10 times compared to that of traditional electrospinning. The morphology observation by scanning electron microscopy showed similar morphology and diameter distribution of the dihydromyricetin or eugenol nanofibers. Fourier transform infrared spectra analysis indicated that eugenol or dihydromyricetin interacted with proteins through hydrogen bonding. X-ray diffraction profiles of the nanofibers indicated that dihydromyricetin was uniformly distributed within the nanofibers. Nanofibers encapsulated with various concentrations of eugenol or dihydromyricetin showed better barrier properties against water vapors than the free nanofibers, which indicated that the hydrophobic eugenol and dihydromyricetin inhibited the permeation of water molecules through the nanofibers. Tensile tests showed that the nanofibers loaded with 0.5% eugenol or 1.0% dihydromyricetin exhibited relatively higher elastic moduli and lower elongation at break. The nanofibers loaded with dihydromyricetin showed comparable antioxidant activity to that with eugenol in the aspect of ferric and cupper reducing activity, while that with 0.1%–1.0% dihydromyricetin showed superior DPPH radical scavenging activity. Furthermore, both eugenol and dihydromyricetin loaded gelatin/zein nanofibers showed effective antimicrobial activities against Escherichia coli and Staphylococcus aureus . Overall, the dihydromyricetin loaded gelatin/zein nanofibers showed comparable physical and functional properties compared to eugenol, which suggests the application of dihydromyricetin nanofibrous films on food active packaging and the potential application of dihydromyricetin on functional food delivery systems.
研究领域:
材料科学
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食品科学
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纳米技术
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy
·
2017
·
IF 1.059
Juan Wu,Xiao’ai Fang,Yan Yuan,Yanfen Dong,Yanling Liang,Qingchun Xie,Junfeng Ban,Yanzhong Chen,Zhufen Lv
Canarium pimela K.D. Koenig, Burseraceae, have a long history of use in the Chinese traditional medicine treatment of various ailments including hypertension, and our research team has reported the anti-hypertensive activity and delineated the mechanism involved in the action. The following research aims to evaluate the vasorelaxant and antioxidant activities of ethanol extract from C. pimela leaves and to analyze its chemical composition by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) that may correlate with their pharmacological activities. The results showed that pre-incubation of aortic rings with the extract (0.3, 1, 3, 10, 30 and 100 mg/l) significantly inhibited the contractile response of the rings to norepinephrine-induced contraction ( p < 0.01or p < 0.05). Crude ethanol extract and refined ethanol extract showed a highest inhibitory effect against 2,2dipheyl-2-picrylhydrazyl hydrate scavenging activity (IC 50 of crude ethanol extract = 15.42 ± 0.14 μg/ml and IC 50 of refined ethanol extract = 5.72 ± 0.31 μg/ml) and 2,2′-azinobis (3-ethyl-benzothiazoline-6-sulphonic acid ammonium salt) (ABTS (IC 50 of crude ethanol extract = 3.24 ± 0.18 μg/ml and IC 50 of refined ethanol extract = 1.88 ± 0.07 μg/ml) scavenging activity, which was considerably higher than that reported for butylated hydroxytoluene and lower of that measured for ascorbic acid. Moreover, its chemical composition was analyzed by UPLC/Q-TOF-MS. Sixteen compounds including nine flavonoids, four tannins, two phenolic acids and one dianthrone were identified for the first time as constituents of this species. And of this, six major phenolic components were simultaneous quantitative analysis by HPLC-UV, chlorogenic acid is the major compounds in C. pimela leaves. These results indicate that the phenolic-rich extract of C. pimela leaves is a promising natural pharmaceutical for combating hypertension and oxidative stress.
研究领域:
药理学
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分析化学
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传统中药
✓ 已验证
产品关联
DOI
高影响因子
Fermentation-Basel
·
2023
·Vol. 9
·期 4
·322
·
IF 3.7
Jian Wang,Quanjin Jiang,Zhenyu Huang,Yan Wang,Hynek Roubik,Kai Yang,Ming Cai,Peilong Sun
Soybean meal is a class of by-products obtained from the processing of soybean products. Despite its high nutritional value, the presence of glycoside isoflavones limits human use of soybean meal. This study evaluated the effect of solid-state fermentation (SSF) with different edible mushroom mycelia (Pleurotus ostreatus,Hericium erinaceus, andFlammulina velutipes) on the proximate composition, antioxidant properties, and physicochemical properties of fermented soybean meal powder (SP). The results revealed that fermented SP had a higher nutritional value when compared to SP.P. ostreatuswas the most pronounced among the three species. Crude protein content was found to have increased by 9.49%, while the concentration of glutamate and aspartic acid increased by 23.39% and 23.16%, respectively. SSF process significantly increased the total polyphenol content (TPC) and aglycone isoflavone content by 235.9% and 324.12%, respectively, resulting in increased antioxidant activity (evaluated by the DPPH, •OH, ABTS+assays). Microstructural changes in fermented SP and nutrient degradation and utilization were observed. Thus, fermented SP can be used as a raw material with enhanced nutritional properties to develop new functional foods, such as plant-based foods represented by plant meat. It provides a promising approach for increasing the added value of soybean meal.Keywords:soybean meal;solid-state fermentation;edible mushrooms;nutritional value
研究领域:
食品科学
·
微生物学
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营养学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Zhiyong Xue,Juan Liu,Qing Li,Yuanyuan Yao,Yalin Yang,Chao Ran,Zhen Zhang,Zhigang Zhou
Lipoic acid ferulate (LAF) was synthesized and its anti-free radical ability in vitro was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonicacid) (ABTS) assays. Protective effects of LAF in stabilizing fish oil were tested, compared to antioxidants such as lipoic acid, ferulic acid and tert -butylhydroxyquinone (TBHQ) by measuring peroxide values, thiobarbituric acid reactants, p -anisidine values, nuclear magnetic resonance (NMR) spectra and gas chromatography–mass spectrometry (GC–MS) spectra of fish oil during accelerated storage (12 days, 80 °C). The inhibitory effect of these antioxidants on fish oil oxidation followed the order TBHQ ≧ LAF > ferulic acid > lipoic acid. In addition, the omega-3 polyunsaturated fatty acids were the first to be oxidized. The formation of oxidation products followed a first-order kinetic model, and the addition of LAF effectively reduced the reaction rate constants. Therefore, LAF can effectively slow down the formation of oxidative products and prolong the shelf life of fish oil.
研究领域:
食品科学
·
化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）