International Journal of Nanomedicine
·
2025
·
IF 6.5
Ziming Ren,Yuelei Zhang,Yanyan Liu,Gang Wang
Purpose The formation of biofilms hinders direct contact between antibiotics and bacteria, reducing the efficacy of antibiotic therapy and presenting a substantial obstacle to the treatment of chronic osteomyelitis. To address this problem, we successfully constructed a chemically and structurally robust vancomycin-copper-tannic acid (Van@CuTA) antibiotic sustained-release system via a simple synthetic method, to target bacteria and inhibit biofilm formation.Methods Tannic acid (TA), copper ions (Cu2+), and vancomycin hydrochloride (Van) were mixed and stirred at a low temperature to afford Van@CuTA nanospheres. Their antibacterial activity was evaluated against methicillin-resistant S. aureus (MRSA) using the Oxford cup method and live/dead bacterial staining. Their anti-biofilm activity was assessed via FE-SEM observations. Cell viability was assessed via MTT assay and osteogenic activity was detected via Western blotting (WB) and alkaline phosphatase (ALP) staining after co-culture with MG-63 cells. In vivo tibia-induced osteomyelitis model in rabbits was conducted to further validate the efficacy of Van@CuTA in treating osteomyelitis.Results The developed nanospheres exhibited drug release properties, with TA being released from the carrier and inhibiting the formation of bacterial biofilms. In vivo experiments demonstrated inhibition of MRSA biofilm formation through the encapsulation of TA within fibrin and its sustained release, followed by the sequential release of Van and Cu2⁺ to directly eradicate MRSA. Moreover, Van@CuTA effectively promoted cell proliferation and bone formation. Van@CuTA is a multifunctional system that integrates biofilm targeting, drug release, antimicrobial activity, and cuproptosis-like bacterial death.Conclusion This study offers a novel and promising strategy for the development of antimicrobial agents to combat MRSA-induced chronic osteomyelitis.
研究领域:
骨科学
·
感染性疾病
·
纳米医学
·
抗菌材料
·
药物递送系统
·
分子生物学
·
转化医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2025
·
IF 7.9
Feipeng Wu,Weiling Yin,Hui Shan,Jie Cang,Ruixing Shui,Xianwen Yan,Wenbo He,Junmeng Liu,Jia Gao,Dapeng Li,Guoqing Pan
Osteosarcoma (OS) constitutes a highly malignant bone tumor, prone to postoperative recurrence and marked bone loss, which presents substantial challenges to current clinical practice. To address these difficulties, we developed a dynamic hydrogel constructed via reversible Cu–S coordination bonds, endowed with injectability, self-healing capacity, and biodegradability.This hydrogel is composed of copper sulfide nanoparticles (CuS 2 NPs), thiolated osteogenic peptide (OGP-SH), and thiolated biomacromolecular gelatin (Gel-SH). The incorporation of copper sulfide nanoparticles imparts strong photothermal conversion properties to the hydrogel, enabling effective ablation of residual osteosarcoma cells upon irradiation with 808 nm near-infrared (NIR) light. Concurrently, the sustained release of osteogenic peptide and trace amounts of copper ions facilitates osteogenic differentiation and promotes bone regeneration. The hydrogel demonstrated excellent injectability, structural stability, rheological recovery capacity, as well as favorable biocompatibility, with a degradation rate of approximately 65 % within 14 days. The results of in vitro and in vivo studies substantiated its dual functionality in suppressing tumor recurrence and enhancing bone repair. The research data reveal that this hydrogel exhibits broad prospects as a multifunctional therapeutic vehicle for postoperative osteosarcoma management and skeletal tissue regeneration.
研究领域:
生物医学工程
·
纳米医学
·
癌症治疗
·
组织工程
·
生物材料
✓ 已验证
产品关联
DOI
高影响因子
ARCHIV DER PHARMAZIE
·
2025
·Vol. 358
·期 11
·e70121
·
IF 3.6
Ling Jiang,Jiangming Zhong,Huixiong Chen,Yi Li,Qi Zhou,Miaohong Yang,Li Lin,Peng Shu,Zhiyun Du
Retinoic acid receptor-related orphan receptor gamma-t (RORγt) is one of the nuclear receptor transcription factors that plays a key role in the differentiation of pro-inflammatory IL-17 + T helper cells. Recently, RORγt has attracted increasing attention as a potential drug target for the treatment of several human inflammatory diseases, including psoriasis and Crohn's disease. In this study, we revealed that ergosterol (ERG) was a novel RORγt inverse agonist, which decreased RORγt transcriptional activity, to regulate collagen fiber deposition and to inhibit pro-inflammatory cytokines, including TNF-α, IL-1β, IL-6, IL-17, IL-22, and IL-23 in the skin lesions of IMQ-induced mice with psoriasis. Furthermore, ERG could repair the epidermal barrier function by upregulating tight junction proteins, such as claudin, occludin, and ZO-1, thereby alleviating psoriasis symptoms. These findings suggested that ERG might be used as a promising novel RORγt inverse agonist in the management of psoriasis.
研究领域:
免疫学
·
皮肤病学
·
分子药理学
·
炎症性疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Biology
·
2025
·Vol. 9
·期 4
·2400512
·
IF 2.6
Xiaolong Zhang,Yushen Wang,Junlei Han,Weilong Zhao,Wenhong Zhang,Xinyu Li,Jun Chen,Wei Song,Li Wang
Heart disease remains a leading cause of mortality worldwide, posing a significant challenge to global healthcare systems. Traditional animal models and cell culture techniques are instrumental in advancing the understanding of cardiac pathophysiology. However, these methods are limited in their ability to fully replicate the heart's intricate functions. This underscores the need for a deeper investigation into the fundamental mechanisms of heart disease. Notably, cardiac pathology is often influenced by systemic factors, with conditions in other organs contributing to disease onset and progression. Cardiac-focused multi-organ chip technology has emerged to better elucidate these complex inter-organ communications and address the limitations of current in vitro models. This technology offers a novel approach by recreating the cardiac microenvironment and integrating it with other organ systems, thereby enabling more precise disease modeling and drug toxicity assessment. This review provides a comprehensive overview of the heart's structure and function, explores the advancements in cardiac organ chip development, and highlights the applications of cardiac-focused multi-organ chips in medical research. Finally, the future potential of this technology in enhancing disease modeling and therapeutic evaluation is discussed.
研究领域:
生物医学工程
·
心血管研究
·
药学科学
·
组织工程
·
微流控技术
·
系统生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2025
·Vol. 23
·期 11
·445
·
IF 5.4
Junming Zhang,Jiangming Zhong,Yi Li,Qi Zhou,Zhiyun Du,Li Lin,Peng Shu,Ling Jiang,Wei Zhou
Background: Ultraviolet B (UVB) radiation induces oxidative stress, inflammation, and collagen degradation in skin, leading to photodamage. Ergosterol (ERG)—a sterol widely distributed in fungi and algae, including numerous marine species—possesses antioxidant and anti-inflammatory activities, but its photoprotective mechanisms remain unclear. Methods: Using integratedin vitro(UVB-irradiated human keratinocytes) andin vivo(topical ERG in a murine UVB model) approaches, combined with transcriptomic and network pharmacology analyses, we evaluated ERG’s effects on oxidative stress, inflammation, and extracellular matrix integrity. Results: ERG treatment preserved keratinocyte viability, reduced reactive oxygen species, and suppressed pro-inflammatory mediators after UVB exposure. In mice, topical ERG significantly attenuated epidermal hyperplasia, maintained tight-junction integrity, and inhibited collagen matrix degradation. Mechanistically, ERG exerted dual inhibition of the nuclear factor kappa B (NF-κB) pathway, which mediates inflammation, and the mitogen-activated protein kinase (MAPK) pathway, which regulates collagen degradation. Conclusions: These findings identify ERG as a marine-derived sterol with potent photoprotective activity that simultaneously targets oxidative stress, inflammation, and extracellular matrix damage, highlighting its promise as a natural compound for dermatological applications and aligning with ongoing efforts to explore marine-derived agents against skin oxidative stress and inflammation.
研究领域:
皮肤病学
·
海洋天然产物
·
药理学
·
分子生物学
·
氧化应激与炎症
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yu Su,Xinjie Yin,Xiaofeng Wei,Rui Xu,Liwen Wei,Yanhua Chen,Lan Ding,Daqian Song
A facile and efficient detection method is required to address the potential health risks of ketoprofen (KP) in animal-derived foods. Herein, we integrated molecularly imprinted polymers (MIPs) with Cu-doped Fe 3 O 4 nanozymes (Fe 3 O 4 –Cu) to develop a selective colorimetric sensor for KP detection. Chitosan and glutaraldehyde were used as functional monomers and cross-linkers to fabricate proposed the MIPs@Fe 3 O 4 –Cu. On KP addition, it was specifically captured by the imprinted cavities, thereby blocking the channels between chromogenic substrates and Fe 3 O 4 –Cu. Based on this rationale, a selective colorimetric sensor utilizing MIPs@Fe 3 O 4 –Cu was established, exhibiting a linear range of 0.25–100 μM and a detection limit of 0.073 μM. The developed method was validated through its application in milk samples, yielding satisfactory recoveries with low relative standard deviations. This efficient and selective colorimetric sensor holds immense significance for KP detection in complex samples.
研究领域:
分析化学
·
纳米技术
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Kaiqi Qin,Xinyue Huang,Shengfeng Wang,Jiachen Liang,Zengjie Fan
Repairing osteochondral (OC) defect presents a significant challenge due to the intricate structural requirements and the unpredictable differentiation pathways of bone marrow mesenchymal stem cells (BMSCs). To address this challenge, a novel biomimetic OC hydrogel scaffold is developed that features a structure of soft and hard components. This scaffold incorporates bilayer metal–organic frameworks (MOFs), specifically ZIF-67 in the upper layer and ZIF-8 in the lower layer, achieved through an in situ printing process. This configuration enables the spatial and temporal modulation of BMSC differentiation by controlling the release of Co 2 ⁺ and Zn 2 ⁺. The results demonstrate that the bilayer MOF hydrogels significantly outperform hydrogels that either lack MOFs or contain a single type of MOF in enhancing repair outcomes in rabbit models of knee OC defects. The improved regenerative efficacy is attributed to the distinct chondrogenic and osteogenic differentiation cues provided by the bilayer MOFs, effectively guiding BMSCs toward enhanced tissue regeneration. This customizable biomimetic OC hydrogel scaffold not only opens new avenues for innovative therapeutic strategies but also holds great promise for widespread clinical applications.
研究领域:
再生医学
·
生物材料科学
·
骨科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Zehao Chen,Binghao Wang,Chengliang Yang,Zhendong Lv,Yu Wei,Tianming Pan,Fuqing Xuan,Xingdie Zhou,Hao Chen,Hongxing Shen,Liqiang Wang,Yuhui Zhang
Effective osteointegration is of great importance for pedicle screws in spinal fusion surgeries. However, the lack of osteoinductive activity of current screws diminishes their feasibility for osteointegration and fixation, making screw loosening a common complication worldwide. In this study, Ti-6Al-4V pedicle screws with full through-hole design were fabricated via selective laser melting (SLM) 3D printing and then deposited with porous oxide coatings by microarc oxidation (MAO). The porous surface morphology of the oxide coating and the release of bioactive ions could effectively support cell adhesion, migration, vascularization, and osteogenesis in vitro. Furthermore, an in vivo goat model demonstrated the efficacy of modified screws in improving bone maturation and osseointegration, thus providing a promising method for feasible orthopedic internal fixation.
研究领域:
生物医学工程
·
骨科
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Chen Xu,Jinhui Lu,Li Zhou,Jie Liang,Lei Fang,Feng Cao
Topical drug delivery system to the posterior segment of the eye is facing many challenges, such as rapid drug elimination, low permeability, and low concentration at the targeted sites. To overcome these challenges, Multifunctional nanocomposite eye drops of dexamethasone-carboxymethyl-β-cyclodextrin@layered double hydroxides-glycylsarcosine (DEX-CM-β-CD@LDH-GS) were developed for relay drug delivery. Herein, our studies demonstrated that DEX-CM-β-CD@LDH-GS could penetrate through human conjunctival epithelial cells with an intact structure and exhibited integrity in the sclera of rabbits’ eyes with in vivo fluorescence resonance energy transfer imaging. Consequently, tissue distribution indicated that DEX-CM-β-CD@LDH-GS nanocomposite eye drops could maintain the effective therapeutic concentration of DEX in choroid-retina within 3 h. As a relay drug delivery system, drug-CD@LDH nanocomposites offer an efficient strategy for drug delivery from ocular surface to the posterior segment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Natural Product Communications
·
2021
·
IF 0.986
Qiang Wang,Chundie Yi,Wenlan Duan,Yunfeng Duan,Jiahao Lou,Guangzhi Zeng,Junlin Yin
Two new sesquiterpenoids, isocyperotundone (1) and 1,4-epoxy-4-hydroxy-4,5-seco-guain-11-en-5-one (2), together with 6 known sesquiterpenoids, cyperotundone (3), cyperenoic acid (4), sugetriol triacetate (5), cyperusol A3 (6), cyperusol A2 (7), and cyperusol A1 (8), were isolated from the methanol extract of the rhizomes of Cyperus rotundus L. High-resolution electrospray ionization mass spectrometry and 1-dimensional (1D) and 2D nuclear magnetic resonance spectroscopy were used to establish the structures of all the compounds. All the compounds were tested for activity on nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling. Compounds 1-7 exhibited inhibitory activity on tumor necrosis factor-α-induced activation of the NF-κB pathway, with half-maximal inhibitory concentration values ranging from 34.5 to 73.7 μmol/L.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Immunology Research
·
2021
·
IF 4.818
Lin Zhang,Lukun Yang,Xiaowei Xie,Hongyue Zheng,Hangsheng Zheng,Lizong Zhang,Cuizhe Liu,Ji-Gang Piao,Fanzhu Li
Baicalin (BA) magnesium salt (BA-Mg) is a good water-soluble ingredient extracted from Scutellaria baicalensis Georgi, a commonly used traditional Chinese medicine. This study is aimed at investigating whether BA-Mg could exert a better protective effect on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice and illuminate the underlying mechanisms in vivo and in vitro. Mice were intraperitoneally administrated with equimolar BA-Mg, BA, and MgSO4 before LPS inducing ALI. Lung tissues and bronchoalveolar lavage fluid were collected for lung wet/dry ratio, histological examinations, cell counts, and biochemical analyses at 48 h post-LPS exposure. Meanwhile, the protein expressions of TLR4/NF-κB signaling pathway and proinflammatory cytokines in lung tissues and lung bronchial epithelial cells (BEAS-2B) were detected. The results showed BA-Mg pronouncedly ameliorated LPS-induced inflammatory response and histopathological damages, elevated antioxidant enzyme activity (SOD), and downregulated myeloperoxidase (MPO) and malonaldehyde (MDA) levels through the inhibition of TLR4/NF-κB signaling pathway activation. Moreover, the effect of BA-Mg was significantly better than that of BA and MgSO4 in ameliorating symptoms. Overall, BA-Mg can effectively relieve inflammatory response and oxidative stress triggered by LPS, indicating it may be a potential therapeutic candidate for treating ALI.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Huiyi Yang,Qiyi He,Junkang Pan,Mingxia Lin,Zhiting Lao,Qinglan Li,Xiping Cui,Suqing Zhao
Nanozymes are a type of artificial enzymes that can replace traditional natural enzymes for biotechnology to biosensing, biocatalysis , and biological imaging. Nevertheless, it is still a huge challenge in the pursuit of new mimic enzymes or multiple enzyme-like activities mimics. In this work, PtCu nanocages (PtCu NCs) with hollow interior and rough exterior was prepared. PtCu NCs displayed superior tetra-enzyme mimetic activities including peroxidase (POD)-, superoxide dismutase (SOD)-, catalase (CAT)- and ascorbic acid oxidase (AAO)-like activities. Meaningfully, attributed to the POD mimic activity, sensitive and convenient colorimetric biosensors for L -Cysteine ( L -Cys), homocysteine (Hcy) and glutathione (GSH) were developed with wide linear range (LR) and low limit of detection (LOD) of 0.28 μM, 1.48 μM and 0.75 μM (S/N = 3), respectively. Moreover, based on the AAO mimic activity, the cascade reaction was proposed including the oxidation of ascorbic acid (AA) to dehydroascorbic acid (DHAA) by PtCu NCs and the formation of the fluorescent quinoxaline (DFQ) coming from the reaction between DHAA and o-phenylenediamine (OPD). A novel fluorescent biosensor for dehydroepiandrosterone (DHEA) was successfully constructed based on the immunoassay method combined with the cascade reaction for AA, with the LR of 0.6–464.9 ng·mL −1 and LOD of 0.5 ng·mL −1 . These colorimetric and fluorescent biosensors displayed outstanding performance in terms of sensitivity, specificity, and accuracy for real samples, suggesting that PtCu NCs was a promising candidate for biosensing.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）