Journal of the American Chemical Society
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2009
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IF 8.091
Yinghua Peng,Xiaohui Wang,Yi Xiao,Lingyan Feng,Chao Zhao,Jinsong Ren,Xiaogang Qu
The increasing worldwide demand for carbon nanotubes (CNTs) and increasing concern regarding how to safely develop and use CNTs are requiring a low-cost, simple, and highly sensitive CNT detection assay for toxicological evaluation and environmental monitoring. However, this goal is still far from being achieved. All the current CNT detection techniques are not applicable for automation and field analysis because they are dependent on highly expensive special instruments and complicated sample preparation. On the basis of the capability of single-walled carbon nanotubes (SWNTs) to specifically induce human telomeric i-motif formation, we design an electrochemical DNA (E-DNA) sensor that can distinguish single- and multiwalled carbon nanotubes both in buffer and in cell extracts. The E-DNA sensor can selectively detect SWNTs with a direct detection limit of 0.2 ppm and has been demonstrated in cancer cell extracts. To the best of our knowledge, this is the first demonstration of a biosensing technique that can distinguish different types of nanotubes. Our work will provide new insights into how to design a biosensor for detection of carbon nanotubes.
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MUTAGENESIS
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2025
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IF 4.3
Hu Yushi,Lei Yuyang,Gao Zhenna,Cao Yiyi,Xi Jing,Ma Yuning,Gao Qinwen,Fu Jian,Zhang Xinyu,Luan Yang
Aristolochic acid I (AAI) is a carcinogen associated with various human cancers. However, its causal relationship with hepatocellular carcinoma remains controversial, and inconsistent results from rodent studies have suggested species-specific differences. Here we evaluated AAI genotoxicity using functional human-induced hepatocyte-like cells (hiHep cells), a model that closely mimics primary human hepatocytes in gene expression and function, thereby shedding light on its potential hepatocarcinogenic risk in humans. First, we assessed AAI genotoxicity by evaluating AAI-DNA adducts and micronucleus frequency. In hiHep cells, AAI (0.7–2.5 µM) induced up to 105 adducts per 108 nucleotides, indicating high metabolic activation of AAI. A concentration-dependent increase in micronucleus frequency indicated a significant increase in chromosomal aberrations in hiHep cells. Considering the evidence of AAI inducing oxidative stress, we assessed 8-hydroxy-2′-deoxyguanosine and reactive oxygen species levels to evaluate DNA oxidative damage. For both indicators, significantly elevated levels were observed. A mechanism involving oxidative damage was further supported by observations of mitochondrial dysfunction, including changes in mitochondrial membrane potential and mitochondrial complex activity. Ascorbate treatment decreased AAI-induced oxidative DNA damage and DNA adduct formation, providing direct cellular evidence for free radical intermediates in AAI metabolic activation—a mechanism previously hypothesized but not experimentally validated in a human-relevant hepatocyte model. Our study findings revealed the genotoxic effects of AAI on hiHep cells and implicated oxidative stress as the key mechanism. These findings strengthen the association between AAI exposure and liver disease and highlight the potential role of antioxidant therapies in mitigating AAI-associated carcinogenesis.
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TRANSITION METAL CHEMISTRY
·
2015
·Vol. 40
·期 7
·723-732
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IF 1.306
Xu Sheng-Xian,Wang Jing-Lan,Zhao Feng,Xia Hong-Ying,Wang Yi-bo
Three copper(I) complexes, [Cu(PimP)(POP)]PF 6 ( 1 ), [Cu(NimP)(POP)]PF 6 ( 2 ), and [Cu(AimP)(POP)]PF 6 ( 3 ) (PimP = 2-phenyl-1 H -imidazo[4,5-f][1,10]phenanthroline, NimP = 2-(2-naphthyl-1 H -imidazo[4,5-f]phenanthroline, AimP = 2-(9-anthryl)-1 H -imidazo[4,5-f]phenanthroline, POP = bis[2-diphenylphosphino]-phenyl)ether) have been synthesized and characterized. Investigations into the effects of the varying substituents on the structural, absorption, and emission characteristics of the corresponding complexes are presented. Complexes 1 and 2 exhibit similar photophysical properties with the weaker lowest lying MLCT absorption at λ > 400 nm and the phosphorescence 3 MLCT/ 3 LLCT emission in the range of 570–590 nm, whereas complex 3 shows different absorption and emission properties, in which the LLCT absorption band is overlapped with the structured absorption bands belonging to the characteristic peaks of the anthracene moiety, and the emitting excited state is described as the 3 LLCT character. Density functional theory and time-dependent density functional theory calculations were employed to rationalize the photophysical properties of the complexes.
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Nature Communications
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2024
·Vol. 15
·期 1
·1-12
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IF 14.7
He Xiaoyang,Lin Li,Li Xiangying,Zhu Minzhi,Zhang Qinghong,Xie Shunji,Mei Bingbao,Sun Fanfei,Jiang Zheng,Cheng Jun,Wang Ye
The membrane electrode assembly (MEA) is promising for practical applications of the electrocatalytic CO 2 reduction reaction (CO 2 RR) to multi-carbon (C 2+ ) compounds. Water management is crucial in the MEA electrolyser without catholyte, but few studies have clarified whether the co-feeding water in cathode can enhance C 2+ formation. Here, we report our discovery of pivotal roles of a suitable nanocomposite electrocatalyst with abundant Cu 2 O−Cu 0 interfaces in accomplishing water-promoting effect on C 2+ formation, achieving a current density of 1.0 A cm −2 and a 19% single-pass C 2+ yield at 80% C 2+ Faradaic efficiency in MEA. The operando characterizations confirm the co-existence of Cu + with Cu 0 during CO 2 RR at ampere-level current densities. Our studies reveal that Cu + works for water activation and aids C‒C coupling by enhancing formations of adsorbed CO and CHO species. This work offers a strategy to boost CO 2 RR to C 2+ compounds in industrial-relevant MEA by combining water management and electrocatalyst design.
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Environmental Science-Water Research & Technology
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2024
·Vol. 10
·期 8
·1785-1794
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IF 3.5
Jun Zhang,Shenjun Wang,Yuhao Wu,Jiahai Ma
In advanced oxidation systems, the promoting effect of bicarbonate is relatively overlooked. Bicarbonate, as an inorganic anion widely present in natural waters, is extremely important for water treatment. Therefore, bicarbonate was added to a PDS/Cu2+ system to investigate the degradation mechanisms of organic contaminants. The generation of alkyl radicals in the PDS/Cu2+/HCO3− system was first demonstrated through Electron Paramagnetic Resonance (EPR) testing. This study reveals that the introduced bicarbonate ions undergo transformation into alkyl radicals. The presence of alkyl radicals promotes the cycling of monovalent, divalent, and trivalent copper ions, facilitating the degradation of contaminants. The production of monovalent and trivalent copper ions in the reaction system was confirmed through UV-vis absorption spectroscopy and quenching experiments. Furthermore, the change in the oxidation state of copper ions was further confirmed through EPR testing. These findings not only shed new light on the degradation mechanism of the PDS/Cu2+ system in the presence of bicarbonate but also open up new avenues for the further application of bicarbonate in advanced oxidation processes.
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POLYMER
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2024
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IF 4.1
Changzeng Wang,Shuxin Li,Ye Yuan,Yundong Ji,Dongfeng Cao
Polysiloxanes are known to significantly enhance the toughness and thermal stability of epoxy resins . However, the study of phase transitions and their effects on the properties of modified resins, synthesized through copolymerization or blending, was quite limited. In this work, a phenyl propyl polysiloxane (PPPS) modified epoxy resin (P/E) was prepared using a solvent method to scrutinize the influence of varying PPPS concentrations and phase transitions on the macroscopic properties of the resin. The molecular structure of P/E composite was confirmed through Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance ( 1 H and 29 Si NMR) analysis. Based on atomic force microscopy (AFM) results, the progression of phase transitions was deduced: from a homogeneous phase to a co-continuous phase, and subsequently to a sea-island phase. This progression of phase transitions is attributed to the varying solubility of the copolymer with different PPPS content in the resin. At a PPPS content of 20 wt%, a significant improvement in tensile strength (σ) and critical strain energy release rate (GIc) was observed, increasing by 45 % and 400 %, respectively, upon the formation of a co-continuous phase. The presence of a silicone-rich spherical domain within sea-island phase can significantly improve the G Ic of the resins, while this also results in a rapid reduction in tensile strength (σ). Furthermore, the thermal stability of P/E composite is also affected by its phase structures. The silicone-rich spherical domains are more effective in forming a protective layer compared to the co-continuous phase, which leads to a decreased pyrolysis rate and an increased yield of residual char. This work elucidates the influence of PPPS content and phase structure on the macroscopic properties, highlighting the significant potential for developing composite materials with superior comprehensive performance.
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Science Bulletin
·
2025
·
IF 21.1
Wanguo Gao,Zhe Lu,Yecheng Leng,Chengyang Wu,Yingfang Yao,Xi Zhu,Junchuan Sun,Xiwen Yu,Cheng Wang,Congping Wu,Wenlei Wu,Bing Wang,Lu Wang,Zhigang Zou
Cu-based catalysts attract considerable attention because of their exceptional CO 2 photo-/electro-/thermo-reduction capacities, where Cu(I) is generally treated as the active species. However, a significant problem hindering the large-scale applications of Cu-based catalysts is the inactivation of Cu(I) via irreversible redox to Cu(II)/Cu(0). This study proposes a Cu(I) valence pinning method based on hydroxyapatite (HAP). Experimental and theoretical studies demonstrate that the phonon resonance among the Cu(I) ions, their adjacent heteroatoms, and the intermediates adsorbed at the Cu(I) sites yields Cu(I) valence electrons in their lowest energy states. Thus, Cu(I) is stabilized, stable, and efficient photothermal CO 2 hydrogenation is promoted. However, because of the change of Cu(I) coordination environment during the CO 2 hydrogenation reaction, Cu(I) ions migrate into the bulk phase, leading to activity attenuation. Nevertheless, Cu(I) ions can be pulled out to the surface of HAP under the oxidative humid air condition, and the catalytic activity can be easily recovered. Thus, we propose a simple cyclic reaction/regeneration process. This enables the Ca 5 (FeCuCe) 5 -HAP catalyst to achieve the CO yield of 402.8 mmol g −1 h −1 and a CO 2 conversion rate of 27.7%, which is close to the thermodynamic equilibrium. This catalyst also displays a selectivity of approximately 100% and cycle stability of 156 h at 500 °C under a pressure of 1 atmospheric. Our study provides a viable method for the scale applications of Cu(I) based catalysts in the “negative carbon” industries.
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ACS Biomaterials Science & Engineering
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2024
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IF 5.4
Hao Jin,Xuanqi Zhu,Heng Liu,Lu Wang,Shuwei Liu,Hao Zhang
The clinical treatment of bone defects includes allogeneic bone transplantation and autologous bone transplantation. However, they all have their own limitations, and the scope of application is limited. In recent years, bone tissue engineering scaffolds based on a variety of materials have been well developed and achieved good bone regeneration ability. However, most scaffold materials always face problems such as high biotoxicity, leading to inflammation and poor bioactivity, which limits the bone regeneration effect and prolongs the bone regeneration time. In our work, we prepared hydroxyapatite, erythropoietin (EPO), and osteogenic growth peptide (OGP) codoped type-I collagen (Col I) polypeptide nanofiber membranes (NFMs) by electrostatic spinning. In cell experiments, the composite NFMs had low cytotoxicity and promoted osteogenic differentiation of rat bone marrow mesenchymal stem cells. Quantitative real-time polymerase chain reaction and alkaline phosphatase staining confirmed the high expression of osteogenic genes, and alizarin red S staining directly confirmed the appearance of calcium nodules. In animal experiments, the loaded hydroxyapatite formed multiple independent mineralization centers in the defect center. Under the promotion of Col I, EPO, and OGP, the bone continued to grow along the mineralization centers as well as inward the defect edge, and the bone defect completely regenerated in about two months. The hematological and histological analyses proved the safety of the experiments. This kind of design to promote bone regeneration by simulating bone composition, introducing mineralization center and signal molecules, can shorten repair time, improve repair effect, and has good practical prospects in the future.
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PHYTOMEDICINE
·
2026
·
IF 11.3
Baoyu Wei,Hailong Shen,Chengtao Sun,Guangzhao Pan,Qianqian Xu,Xiaoqing Guan,Jiangjiang Qin,Guoyin Kai
Tan I targets HUWE1 and disrupts HUWE1-mediated ubiquitination of NCOA4, leading to NCOA4 stabilization. • Genetic evidence confirms that the HUWE1-NCOA4 axis regulates ferroptotic cell death. • Tan I induces mitochondrial iron overload and dysfunction, partially mediated by MFRN2. • Tan I suppresses gastric cancer growth in vivo and is associated with ferroptosis activation. Background Gastric cancer (GC) remains a major global health challenge, underscoring the urgent need for effective therapeutic strategies. Ferritinophagy-driven ferroptosis has recently emerged as a promising anticancer approach; however, potent pharmacological inducers and their modes of action remain largely unexplored. Purpose This study aimed to investigate whether Tanshinone I (Tan I), a bioactive diterpenoid quinone, exerts antitumor effects against GC via ferritinophagy-dependent ferroptosis and to elucidate its underlying molecular mechanism. Methods Ferroptosis induction was evaluated using pharmacological rescue assays, flow cytometry, confocal microscopy, and transmission electron microscopy. Mechanistic investigations were conducted through quantitative proteomics, immunofluorescence staining, immunoblotting, and analyses of protein stability and ubiquitination. Target identification and validation were employed using limited proteolysis-mass spectrometry, cellular thermal shift assay, drug affinity responsive target stability, computational modeling, co-immunoprecipitation, and genetic manipulation. The antitumor efficacy of Tan I was further evaluated in orthotopic and patient-derived xenograft (PDX) models. Results Tan I was identified as a potent inducer of ferroptosis and ferritinophagy in GC cells, as demonstrated by increased labile ferrous iron and lipid reactive oxygen species, characteristic mitochondrial abnormalities, and partial reversal of Tan I-induced cytotoxicity by ferroptosis inhibitors. Mechanistically, Tan I disrupted the interaction between the E3 ubiquitin ligase HUWE1 and the ferritinophagy cargo receptor NCOA4, thereby attenuating HUWE1-mediated ubiquitination and proteasomal degradation of NCOA4. Stabilization of NCOA4 enhanced ferritin turnover, resulting in iron overload, lipid peroxidation, mitochondrial dysfunction, and ultimately ferroptotic cell death. Notably, HUWE1 knockdown mimicked the key ferroptotic phenotypes induced by Tan I, whereas HUWE1 overexpression or NCOA4 knockdown mitigated these effects. Importantly, Tan I significantly suppressed tumor growth in both orthotopic and PDX models at well-tolerated doses while concomitantly activating ferritinophagy- and ferroptosis-associated markers in vivo . Conclusions By targeting the HUWE1-NCOA4 axis, Tan I induced ferritinophagy-dependent ferroptosis and exerted potent antitumor activity against GC, highlighting its potential as a lead compound for ferroptosis-based cancer therapies. Download: Download high-res image (205KB) Download: Download full-size image
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Nature Communications
·
2026
·
IF 18.1
Huang Zihang,Zhang Zijun,Geng Wan-Rong,Ma Wenbo,Li Yuyang,Zhou Zhanqi,Fu Yi,Zhang Ruian,Sun Yiran,Fang Xuchao,Jiang Xuanyu,Xiong Qin,Lv Xiayi,Du Guanshihan,Li Shangqing,Fang Yanjun,Yang Yang Michael,Huang Yuhui,Ma Xiu-Liang,Wu Yongjun,Liu Kaihui,Hong Zijian,Deng Tianqi,Tian He,Han Gaorong,Ren Zhaohui
Ferroelectric materials with a switchable polarization are appealing for optoelectronic applications because of their above-bandgap photovoltage with a potential to exceed the Shockley-Queisser limit efficiency within classic p-n junctions. However, the intrinsic photovoltaic current in ferroelectrics remains far below the junction ones by orders, hindering their practical applications. Here we report an extremely large photovoltaic current density of 130 mA cm −2 with a photovoltage of 1.31 V under 375 nm light illumination in single-domain lead titanate films. Its photoresponsivity surpasses all available ferroelectric materials by two orders of magnitude. Systematic investigations reveal that an interfacial Ta dopant layer during solution epitaxy drives a polarization converting of the film via the flexoelectric effect, where surface charged Pb vacancy layer was generated for polarization screening. These two layers produce a p-i-n structure throughout the film, accounting for the ultrahigh photovoltaic response. Furthermore, a self-powered X-ray detector made of such film was explored to achieve a sensitivity of 4.73 C Gy air −1 cm −3 , three orders of magnitude higher than that of commercial amorphous Se detectors. Our findings may pave the way for exploring novel detection and imaging devices based on ferroelectric materials.
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CLINICAL BIOCHEMISTRY
·
2026
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IF 2.3
Huifeng Qian,Haiyong Jin,Shunchang Li
Objective To investigate the cause of markedly elevated cardiac troponin I (cTnI) levels that were inconsistent with the clinical presentation in an 18-year-old male patient with bradycardia. Methods Serial dilution analysis, heterophile antibody blocking test (HBT), polyethylene glycol 6000 (PEG6000) precipitation, and recovery rate calculation were performed on the patient samples, and the experiments were repeated three times. Additionally, continuous monitoring of cTnI, cardiac troponin T, myoglobin, creatine kinase (CK) and CK-MB was conducted on the patient. The recovery rate in the control group was derived from 20 troponin-positive patients. Results The serial dilution test showed good linearity. After HBT treatment, the cTnI concentration was 3744 ng/L, while the control tube was 4024 ng/L, showing no significant change. In contrast, cTnI decreased markedly to 142 ng/L after PEG6000 precipitation. The recovery rate was only 5.61%, which was significantly lower than the mean recovery rate of 53.33% observed in the control group. Conclusion The patient was ultimately diagnosed with falsely elevated cTnI caused by macrotroponin interference. This case suggests that when laboratory results are inconsistent with clinical findings, the possibility of analytical interference should be carefully considered, and effective communication between the laboratory and clinicians is essential to avoid misleading diagnostic and therapeutic decisions.
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TALANTA
·
2025
·
IF 6.1
Hongjie Wu,Dirong Gong,Xiaoyu Jia
The uncontrolled disposal of Tl(I) waste comes at a significant economic and environmental cost because of the acute toxicity and high mobility. To resolve the aggregation problem for most reported MnO 2 based adsorbent materials which significantly affected the adsorption efficiency, porous zwitterionic ammonium propyl sulfonate (zw) polyacrylamide hydrogel (PAAm) has been fabricated to disperse nano-MnO 2 as a support. The developed nano-MnO 2 @PAAm-zw exhibited a specific surface area of 5.06 m 2 /g and a pore diameter of 23.8 nm. particularly amenable to removal of Tl(I) for environmental remediation. The factors, including adsorbent MnO 2 incorporation, feeding amount, adsorption time, pH of the solution, Tl(I) initial concentration and existence of competing cations that governing the adsorption performances were fully elucidated. The maximal removal capacity Q e reaches up to 336.5 mg (Tl) g −1 (MnO 2 ) with 99.92 % elimination efficiency and a low residue of 4.5 μg L −1 . The materials exhibit an astonishing robustness against nature matrix and high concentration of cations with adsorption capacity being 928.6–41.43 times larger than various competing counterparts for lab-made and mineral samples, representing the state-of-the-art level of adsorbents. The adsorbent can be regenerated at least five times retaining 86.0 % of original efficiency. A novel concerted mechanistic pathway of retention mainly associated with re-dox reaction is possible. These features offer great potentials for selective and deep elimination of total thallium in water samples.
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