FOOD CHEMISTRY
·
2011
·
IF 3.458
Huanshun Yin,Yunlei Zhou,Xiaomeng Meng,Tiantian Tang,Shiyun Ai,Lusheng Zhu
In this work, a simple and sensitive electrochemical method was developed to determine Sudan I based on magnetic Fe 3 O 4 nanoparticles modified glassy carbon electrode using cyclic voltammetry and differential pulse voltammetry. The sensor exhibited an obviously electrocatalytic activity towards the oxidation of Sudan I, which can be confirmed by the increased oxidation peak current and the decreased oxidation peak potential when compared with the bare GCE. The determination conditions, such as pH, modifier amount, accumulation time and accumulation potential, were optimised. And some kinetic parameters were calculated. Under the optimum experimental conditions, the oxidation current of Sudan I was proportional to its concentration from 0.01 to 1 μM and 1 to 20 μM. The detection limit was estimated to be 0.001 μM ( S / N = 3). The developed method was successfully applied to determine Sudan I content in food samples with satisfactory results.
研究领域:
分析化学
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食品安全
·
纳米材料科学
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产品关联
DOI
PubMed
高影响因子
Journal of Biophotonics
·
2018
·Vol. 12
·期 3
·e201800237
·
IF 3.768
Yubin Liu,Duyang Gao,Mengze Xu,Zhen Yuan
In this study, CuS nanoparticles with optical absorption covering both near-infrared I (NIR-I) and NIR-II biological windows were prepared and served as the contrast agents for multispectral photoacoustic imaging. The physiological parameters including concentrations of deoxyhemoglobin and oxyhemoglobin as well as the water content in the tumor location were quantified based on the multispectral photoacoustic reconstruction method. More importantly, the concentration of CuS nanoparticles/drugs accumulated in the tumor was also recovered after intravenously injection, which are essential for image-guided cancer theranostics. In addition, phantom and in vivo experimental tests were performed to inspect and compare the imaging depth and signal-to-noise ratio (SNR) between the two NIR biological windows. Interestingly, we discovered that a higher SNR was obtained in the NIR-II window than that in the NIR-I window. Meanwhile, the multispectral imaging results also demonstrated that the imaging contrast and penetration depth in the NIR-II window were also significantly improved as compared to those from the NIR-I window.
研究领域:
生物医学工程
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肿瘤学
·
影像学
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产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Ping Wang,Huikai Ma,Yaoyao Xie,Sanqiang Li
In this study, a ratiometric electrochemical aptasensor was proposed for the highly sensitive detection of carcinoembryonic antigen (CEA) with exonuclease I (Exo I)-assisted target recycling amplification. The thiolated methylene blue (MB)-labelled hairpin capture probe (MB-HP) was immobilized on the surface of gold nanoparticles (AuNPs) modified gold electrode (AuE) via Au-S bonding, and the hybridization between MB-HP and ferrocene (Fc)-labelled aptamer (Fc-Apt) formed a duplex structure, leading to Fc molecules approaching to the electrode surface while MB molecules were far away. In the presence of CEA, its specific recognition with the aptamer caused the release of Fc-Apt from the electrode surface and subsequently the formation of a hairpin structure for MB-HP, resulting in both “signal-on” of MB (I MB ) and “signal-off” of Fc (I Fc ). To improve the sensitivity of the aptasensor, an Exo I-assisted target recycling amplification strategy was designed to achieve an amplified ratiometric signal. Under the optimal conditions, the proposed aptasensor showed a linear detection range from 10 pg mL -1 to 100 ng mL -1 with a detection limit of 4.1 pg mL -1 . Furthermore, the stability, reproducibility, selectively and real sample detection ability of the aptasensor were investigated and satisfactory results were obtained.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 8
·1668
·
IF 5.2
Haibo Zhou,Sen Zhang,Lianghua Chen,Yimei Liu,Luhong Shen,Jiuliang Zhang
For many centuries, Gardenia (Gardenia jasminoidesEllis) was highly valued as a food homologous Chinese herbal medicine with various bioactive compounds, including crocin I and geniposide. However, the functional mechanism underlying the hypoglycemic effect of gardenia is absent in the literature. To evaluate the effect of gardenia and its different extracts on type 2 diabetes mellitus (T2DM) in in vivo and in vitro experiments, the dried gardenia powder was extracted using 60% ethanol and eluted at different ethanol concentrations to obtain the corresponding purified fragments. After that, the active chemical compositions of the different purified gardenia fragments were analyzed using HPLC. Then, the hypoglycemic effects of the different purified gardenia fragments were compared using in vitro and in vivo experiments. Finally, the different extracts were characterized using UPLC-ESI-QTOF-MS/MS and the mass spectrometric fragmentation pathway of the two main compounds, geniposide and crocin I, were identified. The experimental results indicated that the inhibitory effect of the 40% EGJ (crocin I) on the α-glucosidase was better than the 20% EGJ (geniposide) in vitro. However, the inhibitory effect of geniposide on T2DM was better than crocin I in the animal experiments. The different results in vivo and in vitro presumed potentially different mechanisms between crocin I and geniposide on T2DM. This research demonstrated that the mechanism of hypoglycemia in vivo from geniposide is not only one target of the α-glucosidase but provides the experimental background for crocin I and the geniposide deep processing and utilization.Keywords:gardenia;α-glucosidase;crocin I;geniposide;type 2 diabetes mellitus
研究领域:
药理学
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药物化学
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糖尿病研究
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Yun Gao,Tong Xuan,Fu Chen,Yiping Wu,Xiaoyu Guo,Ying Wen,Haifeng Yang
As one of the most potent carcinogens, aristolochic acid I (AAI) can be founded in many plants, which are widely used as raw materials in traditional Chinese medicine. Surface enhanced Raman scattering (SERS) method would be developed to detect the AAI in rapid way. However, hydrophobic AAI molecule with small scattering section hardly approaches onto the surface of metal nanoparticles, resulting in poor Raman detection sensitivity. In this work, bovine serum albumin (BSA) decorated silver nanoparticles (Ag NPs) with core-shell structure (Ag@BSA NPs) are successfully synthesized. The molecular docking induced strong interaction between BSA and AAI is validated by molecular simulation and Ag@BSA NPs could actively capture AAI and anchor the target molecule into the hot spots of Ag NPs, which greatly improves SERS response of AAI. The dynamic linear range for SERS detection of AAI is from 0.5 to 50 μM (R 2 = 0.9910) and the lowest detectable concentration of 0.28 μM is reached, which meeting the strict limitation of national standard regulation for AAI in cosmetic products. In addition, the method is applied for AAI determination in skin care products and good results are achieved.
研究领域:
分析化学
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纳米技术
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毒理学
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药学
✓ 已验证
DOI
高影响因子
Nanoscale Advances
·
2022
·Vol. 5
·期 1
·133-141
·
IF 5.598
Shanshan Wei,Shiyong Li,Haolin Xiao,Feijun Zhao,Jianming Zhu,Zhencheng Chen,Liangli Cao
Gastric cancer (GC) is a common malignant tumour of the digestive tract with a high mortality rate worldwide. However, many patients delay treatment due to the avoidance of the costly and painful procedure of gastroscopy. Therefore, an early convenient screening method is essential to improve the survival rate of GC patients. To address this issue, we constructed an electrochemical immunosensor supported by rhombohedral Cu3Pt and MoS2 nanoflowers (MoS2 NFs) for rapid, painless and quantitative detection of the GC biomarker in vitro. Here, pepsinogen I was employed as a model protein biomarker to analyse the performance of the immunosensor. The rhombohedral dodecahedral Cu3Pt nanoparticles decorated with MoS2-NFs were further functionalized; this allowed the constructed sensor to possess more nano- or micro-structures, thereby improving the detection sensitivity. In specific applications, the corresponding bioactive molecules can be flexibly captured. Under optimal conditions, the immunoassay showed a wide linear range from 500 pg mL−1 to 400 ng mL−1 and a low detection limit of 167 pg mL−1 (S/N = 3). This covers the critical value of 70 ng mL−1, and the results obtained from the analysis of human serum samples were on par with those from the enzyme immunoassay, suggesting significant potential for this new method in daily diagnosis.
研究领域:
生物医学工程
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癌症研究
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Xiang Liu,Wenkai Zhang,Wenzhao Shang,Xianchao Du,Hongjian Liu,Ben Zhong Tang,Dongfang Qiu,Ruijin Yu,Huifang Su
Synergistic type I photodynamic therapy (PDT) and photothermal therapy (PTT) offer a prospective approach to address the challenges posed by tumor hypoxia and heterogeneity, thereby achieving enhanced antitumor therapeutic outcomes. However, developing simple synthetic phototheranostic agents with efficient type I reactive oxygen species (ROS) generation and effective photothermal conversion is still a significant task. In this study, fluorenone (FO), known for its potent electron-withdrawing properties, was conjugated with the rotor-type electron-donating triphenylamine (TPA) to construct a donor–acceptor–donor (D–A D) structural aggregation-induced emission (AIE) photosensitizer ( TPAF ), followed by the formation of nanoparticles (NPs) TPAF CNPs via self-assembly with DSPE-mPEG 2000 -cRGD and DSPE-mPEG 2000 . The TPAF CNPs effectively integrated efficient type I ROS with exceptional photothermal conversion capability. The fundamental photophysical and photochemical mechanisms underlying the synergistic effects of type I photodynamic and photothermal processes were explored through experimental and theoretical methods. In vitro and in vivo experiments showed that TPAF CNPs exhibited excellent photostability, good biocompatibility, enhanced tumor targeting, and outstanding synergistic type I PDT/PTT effects, achieving substantial antitumor therapeutic efficacy with a single injection and one laser irradiation. Overall, this study contributes to the growing field of high-performance type I PDT/PTT nanomedicine for synergistic phototheranostic applications.
研究领域:
肿瘤学
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纳米医学
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光生物学
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转化医学
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药物递送
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分子影像
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
·
2013
·
IF 2.231
Ming Cong,Cui-Min Hu,Yun-Feng Cao,Zhong-Ze Fang,Shu-Hong Tang,Jia-Rui Wang,Jun-Sheng Luo
Danshen is one of the most famous herbs in the world, and more and more danshen–prescribed drugs interactions have been reported in recent years. Evaluation of inhibition potential of danshen's major ingredients towards UDP-glucuronosyltransferases (UGTs) will be helpful for understanding detailed mechanisms for danshen–drugs interaction. Therefore, the aim of the present study is to investigate the inhibitory situation of cryptotanshinone and dihydrotanshinone I towards UGT enzyme-catalyzed propofol glucuronidation. In vitro the human liver microsome (HLM) incubation system was used, and the results showed that cryptotanshinone and dihydrotanshinone I exhibited dose-dependent inhibition towards HLM-catalyzed propofol glucuronidation. Dixon plot and Lineweaver–Burk plot showed that the inhibition type was best fit to competitive inhibition type for both cryptotanshinone and dihydrotanshinone I. The second plot using the slopes from the Lineweaver–Burk plot versus the concentrations of cryptotanshinone or dihydrotanshinone I was employed to calculate the inhibition parameters (K i ) to be 0.4 and 1.7 μM, respectively. Using the reported maximum plasma concentration (C max ), the altered in vivo exposure of propofol increased by 10% and 8.2% for the co-administration of dihydrotanshinone I and cryptotanshinone, respectively. All these results indicated the possible danshen–propofol interaction due to the inhibition of dihydrotanshinone I and cryptotanshinone towards the glucuronidation reaction of propofol.
研究领域:
药理学
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中药学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Qiumin Ye,Xiaohui Chen,Jing Yang,Datong Wu,Jianfeng Ma,Yong Kong
In this study, a sensitive and selective electrochemical sensing platform for the detection of Sudan I was developed with CuO nanoparticles-decorated 3D N -doped porous carbon (CuO/3DNPC). 3D N -doped porous carbon (3DNPC) was first prepared by calcining the precursors of 3D integrated polysaccharides, and then it was decorated with CuO nanoparticles by hydrothermal method. The CuO/3DNPC modified electrode showed high sensitivity and wide linear range for the detection of Sudan I due to the excellent electrocatalytic activity of CuO and the accelerated electron transfer by 3DNPC. In addition, the proposed CuO/3DNPC also exhibited high selectivity, high reproducibility and stability in the detection of Sudan I. Finally, the CuO/3DNPC based Sudan I sensor was applied in the detection of Sudan I in ketchup and chilli sauces, and the results demonstrated the potential of the developed CuO/3DNPC as a promising electrode material for accurate assay of Sudan I in food samples.
研究领域:
分析化学
·
食品科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 35
·4555-4562
·
IF 3.1
Yu Cao,Haizhu Shi,Zhuo Lan,Kunlin He,Qian Chen,Chungu Zhang,Shun Feng,Lianhai Shan
Screening bioactive compounds from natural products is one of the most effective ways for new drug research and development. However, obtaining a single extract component on a large scale and with high purity from a complex matrix is still an arduous and challenging task. Herein, one metal mediated magnetic molecularly imprinted polymer (mMIP) was rationally designed and prepared for specifically capturing Aristolochic acid I (AAI). The preparation was done with copper(II) as binding pivot, (3-aminopropyl) triethoxysilane as functional monomer, and Fe3O4 as core, by a one-step sol–gel method. Under the optimized conditions, the apparent maximum binding amount of copper mediated mMIP (Cu-mMIP) reaches as high as 349.72 mg g−1, the highest among the reported AAI-MIPs. Moreover, the nanoparticles exhibit excellent specificity and selectivity, good reproducibility and stability, high superparamagnetism (60.32 emu g−1), and high imprinting efficiency (an imprinting factor of 7). By simulating an industrial-scale separation, 16.56 mg AAI (purity of 95.11%) is obtained after six cycles with 100 mg nanoparticles from 20 g Caulis aristolochiae manshuriensis (Guan-mu-tong). Notably, this takes only 3 hours and consumes 50 mL of methanol. The study provides a potent tool for the green, fast, and specific extraction of high-purity ingredients from natural plants in the manufacturing industry and conventional analysis in the lab.
研究领域:
药学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Tingting Song,Hong Wang,Yue Liu,Rongshan Cai,Dezhi Yang,Yongai Xiong
Background Ziyuglycoside I (ZgI), an active ingredient isolated from traditional Chinese medicine Sanguisorba officinalis L, has been demonstrated to increase the leucocytes and protect hematopoietic stem cells. However, the poor solubility and a short half-life of ZgI limit its bioavailability and efficacy. The D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) has been widely used to increase the solubility, improve the encapsulation rate, and extend the half-life of drugs.Methods Here, we formulated the TPGS-modified long-circulating liposomes loading ZgI with a sustained drug release and enhanced therapy for myelosuppression. ZgI-TPGS-liposomes were manufactured using a thin-film hydration technique, followed by characterizations of physicochemical properties, including the particle size, zeta potential, TEM, SEM, FTIR, XRD, stability, drug loading (DL), encapsulation efficiency (EE). The in vitro and in vivo delivery efficiency were further evaluated by cellular uptake, in vitro drug release and in vivo pharmacokinetics. Finally, therapeutic effect on myelosuppression was investigated.Results The ZgI-TPGS-liposomes had an particle size of 97.89 ± 1.42 nm and ZP of −28.65 ± 0.16 mV. It exhibited DL of 9.06 ± 0.76% and EE of 92.34 ± 3.83%, along with excellent storage stability, cellular uptake and sustained drug release to free ZgI and liposomes without TPGS. Additionally, the TPGS modified liposomes significantly enhanced the therapeutic effect of ZgI on CTX induced myelosuppression, which can be confirmed in the apoptosis inhibition and cell viability promotion of CTX injured HSPC-1 cells. Also, the mice in vivo pharmacodynamics demonstrated that TPGS liposomes promoted ZgI increasing the numbers of leucocytes and neutrophils in myelosuppression mice induced by CTX.Conclusion Our research suggest that TPGS-modified long-circulating liposomes loading ziyuglycoside I has potential application in myelosuppression therapy.
研究领域:
药剂学
·
药理学
·
纳米技术
·
中药学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Xuanyu Sha,Siqingaowa Han,Guoqiang Fang,Nan Li,Dianyang Lin,Wuliji Hasi
In this work, a facile and effective method for assembly and transfer nanoparticles on thin layer chromatography (TLC) plate was proposed and used to combined with layer chromatography-surface-enhanced Raman spectroscopy (TLC-SERS) strategy for detection of Rhodamine B and Sudan I. First, Monodispersed Ag NCs with broadly tunable diameters (from 41 to 67 nm) and 7.25 × 10 5 maximum actual enhancement factor (AEF) were successfully prepared and systematically investigated for its optic performance use in TLC-SERS. Au@Ag NCs were monitored by TEM, SEM and UV–Vis spectrometer. The optical properties of Ag NCs were simulated and the experimental results showed good consistency with simulation results of absorption spectra. Through three phases interfacial technique and a simple transfer method, nanostructures were assembled on the TLC plate. Moreover, the semi-quantitative analysis of Rhodamine B and Sudan I in the range of 1–200 ppm was performed based on the assembled TLC plate with the limit of detection as low as 1 ppm. In addition, the practical feasibility of this strategy was verified by the detection of Sudan I and Rhodamine B in chili oil. In general, this assembled TLC-SERS strategy builds a connection for the application of nanomaterials with rich morphology in TLC-SERS, which has a great application prospect and practical application value.
研究领域:
分析化学
·
食品安全检测
·
纳米技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）