STEROIDS
·
2019
·
IF 2.136
Shu-Qi Dong,Shuang-Shuang Wang,Ji-Xiao Zhu,Rong-Hao Mu,Cheng-Fu Li,Di Geng,Qing Liu,Li-Tao Yi
Our previous study has demonstrated that oleanolic acid produced an antidepressant-like effect in mice exposed to chronic stress. Considering that serine/threonine-protein kinase 1 (SGK1) is involved in stress response, the present study aimed to evaluate the involvement of SGK1 in the antidepressant-like effects of oleanolic acid in depression-like mice induced by long term corticosterone (CORT) injection. Behaviors, SGK1, brain-derived neurotrophic factor (BDNF) and its downstream targets were assessed after administration with oleanolic for three weeks. The results indicated that oleanolic acid increased the sucrose preference and decreased the immobility time. In addition, oleanolic acid decreased SGK1 and activated BDNF-AKT/mTOR signaling in the hippocampus of CORT-induced animals. However, we found that GSK650394, an inhibitor of SGK1 did not exert any effects on the behaviors, GR levels and BDNF signaling. The number of spines in hippocampal neurons was not changed by GSK650394 as well. Taken together, this study demonstrated that oleanolic acid produced the antidepressant-like effects, which might be related to the down-regulation of SGK1. However, inhibition of SGK1 directly lacks the effects in the treatment of depression.
研究领域:
药理学
·
神经科学
·
精神病学
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DOI
PubMed
高影响因子
MOLECULAR REPRODUCTION AND DEVELOPMENT
·
2021
·Vol. 88
·期 6
·405-415
·
IF 2.609
Li Ren,Yanwen Zhang,Yining Xin,Guo Chen,Xiaoxiao Sun,Yingqi Chen,Bin He
The effect of stress on male fertility is a widespread public health issue, but less is known about the related signaling pathway. To investigate this, we established a hypercortisolism mouse model by supplementing the drinking water with corticosterone for four weeks. In the hypercortisolism mice, the serum corticosterone was much higher than in the control, and serum testosterone was significantly decreased. Moreover, corticosterone treatment induced decrease of sperm counts and increase of teratozoospermia. Increased numbers of multinucleated giant cells and apoptotic germ cells as well as downregulated meiotic markers suggested that corticosterone induced impaired spermatogenesis. Further, upregulation of macrophage-specific marker antigen F4/80 as well as inflammation-related genes suggested that corticosterone induced inflammation in the testis. Lactate content was found to be decreased in the testis and Sertoli cells after corticosterone treatment, and lactate metabolism-related genes were downregulated. In vitro phagocytosis assays showed that the phagocytic activity in corticosterone-treated Sertoli cells was downregulated and accompanied by decreased mitochondrial membrane potential, while pyruvate dehydrogenase kinase-4 inhibitor supplementation restored this process. Taken together, our results demonstrated that dysfunctional phagocytosis capacity and lactate metabolism in Sertoli cells participates in corticosterone-induced impairment of spermatogenesis.
研究领域:
内分泌学
·
生殖生物学
·
免疫学
·
分子生物学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Yuqian Huang,Rongfeng Tang,Peng Xiao,Bo Che,Yan Wang,Huihui Gao,Gang Wang,Changfei Zhu,Tao Chen
Sulfuration plays a decisive role in enhancing crystal growth and passivate defects in the fabrication of high-efficiency metal-sulfide solar cells. However, the traditional sulfuration process always suffers from high-price professional equipment, tedious processes, low activity of S, or high toxicity of H2S. Here, we develop a desired in situ sulfuration by introducing tartaric acid additive into the hydrothermal deposition process of Sb2S3. Tartaric acid, sodium thiosulfate, and potassium antimony tartaric can form Sb2Sx-contained (x > 3) as-prepared films. Encouragingly, the annealing becomes an inspiring in situ sulfuration process, which can obtain a more compact absorber layer. In addition, the crystallinity and defect property of the Sb2S3 film are also improved significantly. Finally, we achieve a high-performance Sb2S3 solar cell with a power conversion efficiency of 6.31%, which shows an encouraging enhancement of ∼15% compared with the traditional hydrothermal process. This study provides an innovative way to prepare high-efficiency Sb2S3 solar cells and provides a desirable guide to realize the in situ sulfuration process.
研究领域:
材料科学
·
能源技术
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DOI
高影响因子
CHEMICAL RESEARCH IN TOXICOLOGY
·
2022
·
IF 3.973
Yanjia Zhao,Chen Sun,Mengdie Su,Junzu Shi,Zixia Hu,Ying Peng,Jiang Zheng
Omeprazole (OPZ) is a proton pump inhibitor commonly used for the treatment of gastric acid hypersecretion. Studies have revealed that use of OPZ can induce hepatotoxicity, but the mechanisms by which it induces liver injury are unclear. This study aimed to identify reactive metabolites of OPZ, determine the pathways of the metabolic activation, and define the correlation of the bioactivation with OPZ cytotoxicity. Quinone imine-derived glutathione (GSH), N-acetylcysteine (NAC), and cysteine (Cys) conjugates were detected in OPZ-fortified rat and human liver microsomal incubations captured with GSH, NAC, or Cys. The same GSH conjugates were detected in bile of rats and cultured liver primary cells after exposure to OPZ. Similarly, the same NAC conjugates were detected in urine of OPZ-treated rats. The resulting quinone imine was found to react with Cys residues of hepatic protein. CYP3A4 dominated the metabolic activation of OPZ. Exposure to OPZ resulted in decreased cell survival in cultured primary hepatocytes. Pretreatment with ketoconazole attenuated the susceptibility of hepatocytes to the cytotoxicity of OPZ.
研究领域:
药理学
·
毒理学
·
药物代谢
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DOI
PubMed
高影响因子
Nanoscale
·
2026
·
IF 5.2
Zemin Zheng,Siyuan Li,Jiuxiang Dai,Ang Li,Xingxing Zhang,Mo Cheng,Zhitong Jin,Lin Zhou
Anisotropic two-dimensional (2D) materials with low structural symmetry and in-plane optical anisotropy provide a promising platform for the development of polarization-driven photonic devices. Herein, high-quality subcentimetre-scale InTeO3Cl single crystals were prepared using the chemical vapor transport (CVT) method and further exfoliated to flakes of nanometer thickness. The crystal structure, optical anisotropy, bandgap, and stability of 2D InTeO3Cl were extensively investigated. Raman characterization verified the strong in-plane optical anisotropy of 2D InTeO3Cl. Furthermore, it presented an optical bandgap of 2.6 eV and excellent environmental and thermal stability. Interestingly, 2D InTeO3Cl manifested an anisotropic second-harmonic generation (SHG) response, which originated from a centrosymmetry breaking induced by periodic interlayer dislocation. Our work reveals the outstanding in-plane optical anisotropy and nonlinear optical effects of 2D InTeO3Cl and provides a promising candidate for the development of high-performance optical and photonic devices.
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产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2026
·
IF 4.7
Hailun Xia,Haoxin Fu,Lu Cao,Ruibin Li,Jun Wu,Peiqi Wang,Ren-ai Xu,Weihong Lin
Albendazole is a broad-spectrum antiparasitic benzimidazole and has potential for the treatment of tumors. The aim of this study was to investigate the potential inhibitory effects of 77 drugs on the metabolism of albendazole, and further to elaborate the inhibitory mechanism of apatinib on albendazole metabolism in vitro and in vivo , where the concentrations of albendazole and its metabolites were detected using an ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) assay. In vitro results showed that 10 drugs inhibited albendazole metabolism by more than 80%, with the half-maximal inhibitory concentrations (IC 50 ) of apatinib in rat liver microsomes (RLM), human liver microsomes (HLM) and recombinant human CYP3A4 (rCYP3A4) being 0.47, 5.53 and 2.01 μM, respectively. Moreover, the inhibition of albendazole by apatinib was non-time-dependent. In addition, the inhibitory mechanisms were mixed consisting of non-competitive and un-competitive for RLM, and competitive and non-competitive for HLM and rCYP3A4, respectively. Pharmacokinetic parameters in Sprague-Dawley rats showed that apatinib caused significant increases in AUC (0-t) , AUC (0-∞) , C max , T max and t 1/2 , while significant decrease in CL z/F for albendazole. For the metabolites albendazole sulfoxide and hydroxyalbendazole, apatinib caused significant increases in AUC (0-t) , AUC (0-∞) , and T max , while significant decrease in CL z/F . In summary, apatinib could inhibit the metabolism of albendazole in vitro and in vivo , so albendazole should be closely monitored for adverse effects when used in combination with apatinib and discontinued if necessary.
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产品关联
DOI
PubMed
高影响因子
POULTRY SCIENCE
·
2024
·
IF 3.8
Min Wu,Guofeng Tan,Ruirui Shi,Dewen Chen,Yumei Qin,Jianzhong Han
ABSTRACT In poultry diets, copper is an essential nutrient that is critical for various physiological functions. Although copper sulfate is commonly used due to its cost-effectiveness, organic copper sources are gaining popularity because of their superior production outcomes and environmental benefits. Nevertheless, understanding the distinct bioaccessibility of inorganic and organic copper in diverse dietary setting remains limited. This study investigated the bioaccessibility of copper sulfate, copper amino acid chelate, and copper proteinate in the intestine via in vitro digestion and in situ dialysis. The results showed significant differences in the molecular size distribution of compounds formed by different copper salts within the intestinal environment, thereby leading to varying bioaccessibility. Copper sulfate has a bioaccessibility of 47 % ± 4%, which is significantly lower than copper amino acid chelate and copper proteinate (63% ± 5%, and 60% ± 4%, respectively) in purified diet systems. Similarly, in whey protein systems, sulfate records 54% ± 10% bioaccessibility compared to 78% ± 9% and 76% ± 5% for copper amino acid chelate and copper proteinate. Coexisting feed ingredients have a significant impact on copper bioaccessibility. Copper sulfate forms precipitates, reducing its bioaccessibility to 34% ± 1% in sodium nitrate solution. The addition of digestive enzyme increases the bioaccessibility of copper sulfate to 81% ± 2% by providing organic ligands. Digestive enzyme also enhanced the bioaccessibility of copper proteinate from 36% ± 4% to 81% ± 4% by degrading its ligands. However, feed ingredients may decrease copper bioaccessibility by forming macromolecular complexes with copper, as all the organic ligands can competitively bind with copper in the intestine. These findings emphasize the importance of considering copper salt types and diet composition in animal nutrition practices.
研究领域:
动物营养学
·
食品科学
·
消化生理学
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DOI
高影响因子
International Journal of Nanomedicine
·
2024
·
IF 6.6
Weitang Sun,Jingbin Zhong,Buyun Gao,Jieling Feng,Zijie Ye,Yueling Lin,Kelan Zhang,Wenqi Su,Shibo Zhu,Yinghua Li,Wei Jia
Purpose Hydroxyapatite-based nanoparticles have found diverse applications in drug delivery, gene carriers, diagnostics, bioimaging and tissue engineering, owing to their ability to easily enter the bloodstream and target specific sites. However, there is limited understanding of the potential adverse effects and molecular mechanisms of these nanoparticles with varying geometries upon their entry into the bloodstream. Here, we used two commercially available hydroxyapatite nanoparticles (HANPs) with different geometries (less than 100 nm in size each) to investigate this issue.Methods First, the particle size, Zeta potential, and surface morphology of nano-hydroxyapatite were characterized. Subsequently, the effects of 2~2000 μM nano-hydroxyapatite on the proliferation, migration, cell cycle distribution, and apoptosis levels of umbilical vein endothelial cells were evaluated. Additionally, the impact of nanoparticles of various shapes on the differential expression of genes was investigated using transcriptome sequencing. Additionally, we investigated the in vivo biocompatibility of HANPs through gavage administration of nanohydroxyapatite in mice.Results Our results demonstrate that while rod-shaped HANPs promote proliferation in Human Umbilical Vein Endothelial Cell (HUVEC) monolayers at 200 μM, sphere-shaped HANPs exhibit significant toxicity to these monolayers at the same concentration, inducing apoptosis/necrosis and S-phase cell cycle arrest through inflammation. Additionally, sphere-shaped HANPs enhance SULT1A3 levels relative to rod-shaped HANPs, facilitating chemical carcinogenesis-DNA adduct signaling pathways in HUVEC monolayers. In vivo experiments have shown that while HANPs can influence the number of blood cells and comprehensive metabolic indicators in blood, they do not exhibit significant toxicity.Conclusion In conclusion, this study has demonstrated that the geometry and surface area of HANPs significantly affect VEC survival status and proliferation. These findings hold significant implications for the optimization of biomaterials in cell engineering applications.
研究领域:
纳米技术
·
生物材料
·
细胞生物学
·
毒理学
·
药学
·
基因组学
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DOI
PubMed
高影响因子
ACS Nano
·
2021
·
IF 15.881
Binbin Wang,Wei Xia,Si Li,Kang Wang,Shengyuan A. Yang,Yanfeng Guo,Jiamin Xue
The ternary van der Waals material Nb2Six–1Te4 demonstrates many interesting properties as the content of Si is changed, ranging from metallic Nb3SiTe6 (x = 5/3) to narrow-gap semiconductor Nb2SiTe4 (x = 2) and with the emergence of one-dimensional Dirac fermion excitations in between. An in-depth understanding of their properties with different stoichiometry is important. Here we use scanning tunneling microscopy and spectroscopy to reveal that Nb2Six–1Te4 is a system with spontaneously developed and self-aligned one-dimensional metallic chains embedded in a two-dimensional semiconductor. Electron quasiparticles form one- and two-dimensional standing waves side by side. This special microscopic structure results in strong transport anisotropy. Along the chain direction the material behaves like a metal, while perpendicular to the chain direction, it behaves like a semiconductor. These findings provide an important basis for further investigation of this intriguing system.
研究领域:
材料科学
·
凝聚态物理
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DOI
高影响因子
CHINESE CHEMICAL LETTERS
·
2021
·
IF 6.779
Haiyan Xiang,Yueshao Zheng,Yang Chen,Yanting Xu,Travis Shihao Hu,Yexin Feng,Yu Zhou,Song Liu,Xuli Chen
Semiconductor electrocatalysis with weak conductivity can accumulate extremely high carriers at semiconductor-electrolyte interface by self-gating effect, which strongly promotes electrocatalytic efficiency. The correlation between semiconductor carrier mobility and electrocatalysis performance is still unclear. Herein atomic-thin transition metal dichalcogenides based composites have been developed for hydrogen evolution reaction (HER) performed with on-chip microdevices. Electrical and electrochemical measurement of individual flack verified the key role of high carrier mobility for enhanced HER activity. Carrier mobility regulation further demonstrated its high dependence with HER performance under self-gating. Our study provides new insight into the carrier mobility of the semiconductor in the electrocatalysis, paving the way for designing high-performance semiconductor catalysts.
研究领域:
材料科学
·
电化学
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DOI
高影响因子
Nano Materials Science
·
2025
·
IF 17.9
Tingfang Xie,Shaodong Sun,Fangyuan Wang,Meiqi Ding,Jie Cui
Interface engineering has emerged as an effective strategy for addressing challenges related to insufficient solar light harvesting and poor photocharge separation in photocatalysis. The distinctive in-plane homogeneous architecture offers the potential for significant enhancements in both photocharge separation and solar light absorption. Herein, a simple one-pot hydrothermal method was developed to construct in-plane homogeneous BiOCl bicrystals within BiOCl nanosheets (BOC-B). During this process, thiourea plays a pivotal role in introducing dislocations to form bicrystal structure. As expected, the synthesized BOC-B samples exhibited substantially higher photocatalytic degradation activity for TC and RhB compared to BOC. Notably, the mechanism underlying effective solar light absorption and charge carrier separation, driven by the homogeneous bicrystal structure, was elucidated through a series of experimental evidences. This work introduces a novel homogeneous bicrystal structure in BiOCl photocatalysts to optimize catalytic activity, providing a framework for developing new BiOCl-based photocatalysts.
研究领域:
材料科学
·
纳米技术
·
环境科学
·
物理化学
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产品关联
DOI
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2015
·Vol. 36
·期 7
·863-873
·
IF 2.912
Lin Qing,Ling Li-qin,Guo Ling,Gong Tao,Sun Xun,Zhang Zhi-rong
Aim: Imperialine is an effective compound in the traditional Chinese medicine chuanbeimu (Bulbus Fritillariae Cirrhosae ) that has been used as antitussive/expectorant in a clinical setting. In this study we investigated the absorption characteristics of imperialine in intestinal segments based on an evaluation of its physicochemical properties. Methods: Caco-2 cells were used to examine uptake and transport of imperialine in vitro , and a rat in situ intestinal perfusion model was used to characterize the absorption of imperialine. The amount of imperialine in the samples was quantified using LC-MS/MS. Results: The aqueous solubility and oil/water partition coefficient of imperialine were determined. This compound demonstrated a relatively weak alkalinity with a pKa of 8.467±0.028. In Caco-2 cells, the uptake of imperialine was increased with increasing pH in medium, but not affected by temperature. The apparent absorptive and secretive coefficient was (8.39±0.12)×10 −6 cm/s and (7.78±0.09)×10 −6 cm/s, respectively. Furthermore, neither the P-glycoprotein inhibitor verapamil nor Niemann-Pick C1-Like 1 transporter inhibitor ezetimibe affected the absorption and secretion of imperialine in vitro . The in situ intestinal perfusion study showed that the absorption parameters of imperialine varied in 4 intestinal segments (duodenum, jejunum, ileum and colon) with the highest ones in the colon, where a greater number of non-ionized form of imperialine was present. Conclusion: The intestinal absorptive characteristics of imperialine are closely related to its physicochemical properties. The passive membrane diffusion dominates the intestinal absorption of imperialine.
研究领域:
药理学
·
中药学
·
药学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）