查找使用阿拉丁产品的科研文献

Embedding Mn2+ in polymer coating on rod-like cellulose nanocrystal to integrate MRI and photothermal function

Yang Shen,Xin Li,Haitao Huang,Yu Lan,Lin Gan,Jin Huang

Grafting Mn ions on 1D particles can produce high-performance MRI agents. However, the motion of Mn ions may cause Mn aggregation and then attenuate imaging contrast. Here, we proposed an MRI-promotion strategy based on embedding Mn 2+ into crosslinked polydopamine (PDA) coating on rod-like cellulose nanocrystals . The results indicate that the slow relaxation of PDA molecular chains restricts the motion of embedded Mn 2+ . When the embedded Mn 2+ content raises up to 2.25 wt%, the particles display a longitudinal relaxivity of 38.08 mM −1 ·s −1 . Such a relaxivity is over 6.6 times of the sample with adsorbed 0.65 wt% Mn 2+ . The aspect ratio of nanoparticles even increases from 14.2 to 20.7 as embedding Mn 2+ concentration increases. Then, the photothermal conversion efficiency of nanoparticles increases from 17.7 % to 44.4 %. This high photothermal conversion efficiency contributes this kind of MRI agent with embedded Mn 2+ to the application potential of integrating tumor diagnosis and treatment.

Metal polyphenol network/cerium oxide artificial enzymes therapeutic nanoplatform for MRI/CT-aided intestinal inflammation management

Zhichao Deng,Wenqi Ma,Chenguang Ding,Chaojun Wei,Bowen Gao,Yuanyuan Zhu,Yujie Zhang,Feng Wu,Mingxin Zhang,Runqing Li,Mingzhen Zhang

Intestinal inflammation, such as ulcerative colitis (UC), is a condition in which there is chronic or recurring inflammation of the gastrointestinal tract . Designing safe, effective, and multifunctional nanomedicines is one of the current difficulties in the treatment of UC. Here we developed a delivery nanosystem based on metal polyphenol network/cerium oxide artificial enzymes (MPN@CeOx) to manage UC and evaluate the severity of inflammation by MRI/CT imaging. MPN@CeOx was engineered by Fe 3+ and epigallocatechin gallate (EGCG) wrapped around CeOx. Increased levels of oxidative stress in the inflammatory lesion facilitated the release of Fe 3+ and EGCG from MPN and liberated CeOx. EGCG with anti-oxidative and anti-inflammatory effects plus CeOx with superoxide dismutase (SOD)-like activity effectively scavenged reactive oxygen species (ROS) and reduced pro-inflammatory cytokines to protect cells from oxidative damage . In addition, H 2 O 2 -responsive releasing of Fe 3+ at the site of inflammation and Ce elements in CeOx enable MRI/CT imaging of UC. In mice colitis models, oral administration of enteric-coated MPN@CeOx alleviated intestinal inflammation and intestinal barrier dysfunction. RNA sequencing analysis revealed that MPN@CeOx ameliorated UC symptoms mainly through inflammation-related signaling pathways . MPN@CeOx with multilevel inflammation management and multimodal imaging capabilities represents an innovative diagnosis and treatment approach for UC.

Manganese-Doped Carbon Dots with Redshifted Orange Emission for Enhanced Fluorescence and Magnetic Resonance Imaging

Sijia Sun,Lining Zhao,Di Wu,Hongxin Zhang,Haichen Lian,Xiaolong Zhao,Aiguo Wu,Leyong Zeng

Metal-doped carbon dots (CDs) exhibited promising application in fluorescence and magnetic resonance (MR) imaging, but developing manganese-doped CDs (Mn-CDs) with long wavelength emission and enhanced MR performance is a challenge. Herein, using a one-step solvothermal method, Mn-CDs with redshifted orange emission and enhanced longitudinal relaxation were synthesized for fluorescence and MR imaging. The results indicated that the prepared Mn-CDs had a uniform size distribution, and the average size was 5 nm. Moreover, Mn-CDs possessed a stronger fluorescence performance than Mn-free CDs, and simultaneously, the emission wavelength can redshift from 542 nm (green emission) to 578 nm (orange emission), owing to the increasing N-doping. Because of the movement limit of Mn2+, Mn-CDs exhibited high T1-weighted MR imaging performance with a longitudinal relaxation rate of 12.69 mM–1·s–1. The in vivo experiments demonstrated their excellent fluorescence and MR imaging with safety and reliability. Therefore, the prepared Mn-CDs with orange emission can be an excellent candidate as a dual-modal nanoprobe for fluorescence and MR imaging.

Glioma-targeted superparamagnetic iron oxide nanoparticles as drug-carrying vehicles for theranostic effects

He-Lin Xu,Kai-Li Mao,Yin-Ping Huang,Jing-Jing Yang,Jie Xu,Pian-Pian Chen,Zi-Liang Fan,Shuang Zou,Zheng-Zheng Gao,Jia-Yu Yin,Jian Xiao,Cui-Tao Lu,Bao-Lin Zhang,Ying-Zheng Zhao

Multifunctional nanoparticles capable of the specific delivery of therapeutics to diseased cells and the real-time imaging of these sites have the potential to improve cancer treatment through personalized therapy. In this study, we have proposed a multifunctional nanoparticle that integrate magnetic targeting, drug-carrier functionality and real-time MRI imaging capabilities in one platform for the theranostic treatment of tumors. The multifunctional nanoparticle was designed with a superparamagnetic iron oxide core and a multifunctional shell composed of PEG/PEI/polysorbate 80 (Ps 80) and was used to encapsulate DOX. DOX-loaded multifunctional nanoparticles (DOX@Ps 80-SPIONs) with a Dh of 58.0 nm, a zeta potential of 28.0 mV, and a drug loading content of 29.3% presented superior superparamagnetic properties with a saturation magnetization (Ms) of 24.1 emu g−1. The cellular uptake of DOX@Ps 80-SPIONs by C6 cells under a magnetic field was significantly enhanced over that of free DOX in solution, resulting in stronger in vitro cytotoxicity. The real-time therapeutic outcome of DOX@Ps 80-SPIONs was easily monitored by MRI. Furthermore, the negative contrast enhancement effect of the nanoparticles was confirmed in glioma-bearing rats. Prussian blue staining and ex vivo DOX fluorescence assays showed that the magnetic Ps 80-SPIONs and encapsulated DOX were delivered to gliomas by imposing external magnetic fields, indicating effective magnetic targeting. Due to magnetic targeting and Ps 80-mediated endocytosis, DOX@Ps 80-SPIONs in the presence of a magnetic field led to the complete suppression of glioma growth in vivo at 28 days after treatment. The therapeutic mechanism of DOX@Ps 80-SPIONs acted by inducing apoptosis through the caspase-3 pathway. Finally, DOX@Ps 80-SPIONs’ safety at therapeutic dosage was verified using pathological HE assays of the heart, liver, spleen, lung and kidney. Multifunctional SPIONs could be used as potential carriers for the theranostic treatment of CNS diseases.

Employing single valency polyphenol to prepare metal-phenolic network antitumor reagents through FeOOH assistance

Mengsi Zhang,Lu Wang,Hao Jin,Nan Zhao,Yi Liu,Shijie Lan,Shuwei Liu,Hao Zhang

Metal-phenolic networks (MPNs) have been conventionally formed by the coordination of metal ions and polyphenols, which can responsively release metal ions and polyphenols under the trigger of tumor microenvironment (TME), showing high potential in the field of antitumor. However, MPNs are mainly limited to multi-valency polyphenols, and the unavailability of single valency polyphenols greatly hinders their applications even though they have excellent antitumor activity . Herein, we demonstrate a FeOOH assisted preparation method for MPNs antitumor reagent by introducing the complexes of Fe 3+ , H 2 O and polyphenol (Fe(H 2 O) x -polyphenol y ) in the preparation process, which overcomes the shortage of single valency polyphenols. Taking apigenin (Ap) as an example, Fe(H 2 O) x -Ap y complexes are foremost formed, in which the Fe(H 2 O) x is capable of hydrolyzing to generate FeOOH, thus producing Fe 3+ -Ap networks-coated FeOOH nanoparticles ( [email protected] NPs). Under the stimulation of the TME, [email protected] NPs could release Fe 2+ and Ap, realizing ferroptosis and apoptosis for tumor combination therapy. In addition, FeOOH can shorten transverse relaxation time, acting as a T 2 -weighted magnetic resonance imaging contrast agent . The current efforts provide an alternative strategy for constructing MPNs by exploiting single valency polyphenols, which strengthens the potential of MPNs in antitumor applications.

Fe3O4 Composite Superparticles with RGD/Magnetic Dual-Targeting Capabilities for the Imaging and Treatment of Non-Small Cell Lung Cancer

Nan Zhao,Qirui Sun,Lening Zhang,Hao Jin,Mengsi Zhang,Shuwei Liu,Hua Xin

In clinical practice, the incidence and mortality of non-small cell lung cancer are increasing year by year, which is a serious threat to the health of patients. Once the optimal surgical window is missed, the toxic side effects of chemotherapy have to be confronted. With the rapid development of nanotechnology in recent years, medical science and health have been greatly impacted. Therefore, in this manuscript, we design and prepare chemotherapeutic drug vinorelbine (VRL)-loaded polydopamine (PDA) shell-coated Fe3O4 superparticles, and further graft the targeted ligand RGD onto their surface. Because of the introduction of the PDA shell, the toxicity of the prepared Fe3O4@PDA/VRL-RGD SPs is greatly reduced. At the same time, due to the existence of Fe3O4, the Fe3O4@PDA/VRL-RGD SPs also have MRI contrast capability. Under the dual-targeting effect of RGD peptide and external magnetic field, Fe3O4@PDA/VRL-RGD SPs can accumulate into tumors effectively. The accumulated superparticles in the tumor sites can not only effectively identify and mark the location and boundary of the tumor under MRI, guideing the application of near-infrared laser, but also release the loaded VRL under the stimulation of the acidic microenvironment of the tumor to play the role of chemotherapy. On further combination with photothermal therapy under laser irradiation, A549 tumors are completely eliminated without recurrence. Our proposed RGD/magnetic field dual-targeting strategy can effectively improve the bioavailability of nanomaterials and contribute to better imaging and therapeutic effects, which has a promising application prospect in the future.

Antioxidant Nanozyme-Engineered Mesenchymal Stem Cells for In Vivo MRI Tracking and Synergistic Therapy of Myocardial Infarction

Wenjun Le,Zeyi Sun,Tieyan Li,Hao Cao,Chuanxue Yang,Tianxiao Mei,Laihai Zhang,Yibing Wang,Wenwen Jia,Wen Sun,Yihui Hu,Zhongmin Liu

Cell therapy is a promising approach for myocardial infarction (MI) treatment. However, this strategy is often restricted by the harsh microenvironment of MI, such as excess ROS, high oxidative stress, inflammation, etc., and thus decreases the curative effect. Additionally, the distribution, migration, and homing of the transplanted stem cells is ambiguous, which also becomes a bottleneck for clinical translation. To address these challenges, herein, a versatile antioxidant nanozyme is designed by polymerically modifying dopamine (PDA) onto the surface of Mn 3 O 4 . The obtained nanozyme acts as an efficient SOD mimic, eliminating ROS, relieving oxidative stress, and reducing inflammation to improve the MI microenvironment. Simultaneously, Mn 3 O 4 @PDA serves as an excellent MRI contrast agent for tracking MSCs. Then, MSCs are engineered with Mn 3 O 4 @PDA nanozyme via endocytosis to form Mn 3 O 4 @PDA-MSCs (Abbr. E-MSCs), which possess superior viability, migration and homing ability compared to normal MSCs. Furthermore, E-MSCs exhibit superior anti-oxidant and anti-inflammatory activity, thereby enhancing the therapeutic efficacy of MSCs. Finally, the in vivo MRI tracking and synergistic therapy potential of E-MSCs is explored in MI model mice. Overall, this work provides a strategy to combine antioxidant activity and imaging properties of nanozymes to simultaneously facilitate imaging tracking of stem cells and improve MI treatment.

Superparamagnetic iron oxide nanoparticles coated with different polymers and their MRI contrast effects in the mouse brains

Songbo Xie,Baolin Zhang,Lei Wang,Jun Wang,Xuan Li,Gao Yang,Fabao Gao

PEG and PEG/PEI modified superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized by the thermal decomposition of iron (III) acetylacetonate (Fe(acac) 3 ) in poly (ethylene glycol) (PEG) containing poly (ethylene imine) (PEI) (0 or 0.3   g). PEG/PEI-SPIONs were coated with Tween 80 (PEG/PEI/Tween 80-SPIONs). Fourier transform infrared spectroscopy (FTIR) analyses indicated that PEG , PEG/PEI and PEG/PEI/Tween 80 were attached to the surfaces of the SPIONs. The PEG-SPIONs, PEG/PEI-SPIONs and PEG/PEI/Tween 80-SPIONs performed excellent colloidal stability in the phosphate buffered saline (PBS), and in deionized water with the mean hydrodynamic sizes of 19.5, 21.0, 24.0   nm and the zeta potentials of -5.0, 35.0, 19.0   mV, respectively. All the SPIONs showed low cytotoxicity assessed by the MTT assay. In vivo magnetic resonance imaging (MRI) of the Kunming (KM) mouse brains were performed, the PEG-SPIONs, PEG/PEI-SPIONs and PEG/PEI/Tween 80-SPIONs exhibited vascular imaging effects in bulbus olfactorius, frontal cortex, temporal, thalamus and brain stem of the mouse brains after 24   h intravenous injection of the nanoparticles . The SPIONs have potentials as MRI contrast agents in the mouse brains.

Glypican-1-antibody-conjugated Gd–Au nanoclusters for FI/MRI dual-modal targeted detection of pancreatic cancer

Xin Huang,Chengqi Fan,Huanhuan Zhu,Wenjun Le,Shaobin Cui,Xin Chen,Wei Li,Fulei Zhang,Yong Huang,Donglu Sh,Zheng Cui,Chengwei Shao,Bingdi Chen

Design, synthesis, and evaluation of VEGFR-targeted macromolecular MRI contrast agent based on biotin–avidin-specific binding

Yongjun Liu,Xiaoyun Wu,Xiaohe Sun,Dan Wang,Ying Zhong,Dandan Jiang,Tianqi Wang,Dexin Yu,Na Zhang

Retracted Article: A tumor-microenvironment fully responsive nano-platform for MRI-guided photodynamic and photothermal synergistic therapy

Daquan Wang,Ning Zhang,Xunan Jing,Yun Zhang,Yanzi Xu,Lingjie Meng

Multifunctional intelligent theranostics agents are promising for next-generation oncotherapy. We fabricated a tumor-microenvironment (TME)-responsive carbon nanotube (CNT)-based nanoplatform for T1 weighted magnetic resonance imaging (MRI)-guided synergistic photodynamic and photothermal therapy (PDT and PTT). CNTs convert near infrared (NIR) radiation into hyperthermia for PTT, and can effectively deliver their cargo into cells due to their unique 1D nanostructure. The CNT@MnO2-PEG@Ce6 nanomedicine was internalized into tumor cells, and rapidly released the photosensitizer (Ce6) in response to the low pH and high glutathione (GSH) levels characteristic of the TME. The degradation of the MnO2 layer under the same conditions released Mn2+ for T1-MRI. Furthermore, catalytic decomposition of the excess H2O2 into oxygen by MnO2 enhanced the efficacy of PDT, relieved hypoxia, and increased consumption of superfluous GSH to mitigate the effects of excessive reactive oxygen species (ROS) generation during PDT. MRI-guided PDT and PTT synergistically inhibited tumor cell growth in vitro, and ablated tumors in vivo. The side effects were negligible due to specific tumor cell targeting via surface modification with folic-PEG, and enhanced permeability and retention. Taken together, CNT@MnO2-PEG is a fully TME-responsive theranostics nanoplatform for targeted tumor ablation and real-time disease tracking.

Mn(II) Complex of Lipophilic Group-Modified Ethylenediaminetetraacetic Acid (EDTA) as a New Hepatobiliary MRI Contrast Agent

Keyu Chen,Pan Li,Chunrong Zhu,Zhiyang Xia,Qian Xia,Lei Zhong,Bin Xiao,Tao Cheng,Changqiang Wu,Chengyi Shen,Xiaoming Zhang,Jiang Zhu

Liver-specific contrast agents (CAs) can improve the Magnetic resonance imaging (MRI) detection of focal and diffuse liver lesions by increasing the lesion-to-liver contrast. A novel Mn(II) complex, Mn-BnO-TyrEDTA, with a lipophilic group-modified ethylenediaminetetraacetic acid (EDTA) structure as a ligand to regulate its behavior in vivo, is superior to Gd-EOB-DTPA in terms of a liver-specific MRI contrast agent. An MRI study on mice demonstrated that Mn-BnO-TyrEDTA can be rapidly taken up by hepatocytes with a combination of hepatobiliary and renal clearance pathways. Bromosulfophthalein (BSP) inhibition imaging, biodistribution, and cellular uptake studies confirmed that the mechanism of hepatic targeting of Mn-BnO-TyrEDTA is the hepatic uptake of the amphiphilic anion contrast agent mediated by organic anion transporting polypeptides (OATPs) expressed by functional hepatocytes.