MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Yang Wang,Jing Peng,Meng Liu,Xin Zhang,Chunrong Li,Yanfei Zhang,Wenjun Duan,Baoping Xie,Zong Dai,Jun Chen
MicroRNAs (miRNAs) are closely associated with the development and progression of cancer, making the development of highly sensitive and rapid miRNA detection strategies a significant challenge. Herein, we developed an electrochemical sensor based on DNA triangular prism (TP) and hybridization chain reaction (HCR) for the ultra-sensitive, rapid, and specific analysis of miRNA. The TP was modified on the electrode surface to obtain high density of recognition probes. After hybridization of the target miRNA with the recognition probes on the TP, HCR was further activated. Numerous biotin-carrying HCR products were generated on the electrode surface. Then the biotin on the HCR products efficiently bound to streptavidin-horseradish peroxidase (SA-HRP), and under the catalysis of a large amount of HRP, TMB and H 2 O 2 rapidly underwent redox reactions, producing the enhanced electrical signal, thus achieving high-sensitivity analysis of miRNA. The developed strategy has a detection limit of 2.21 aM and demonstrates high specificity with the ability to distinguish single-base-pair differences in miRNA. The strategy also exhibits good reproducibility, stability, and practical application potential. We believe that this developed strategy holds promise as an effective tool for miRNA analysis and is expected to play a crucial role in disease diagnosis and new drug development.
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高影响因子
Advanced Science
·
2025
·
IF 14.1
Ruipeng Shi,Wen Tan,Haochun Jin,Sio I Chan,Wei Li,Si San Lei,Guozhen Cui,Yitao Wang,Dong-Hua Yang,Zhangfeng Zhong
Ulcerative colitis (UC) is a condition with complex immune dysregulation and chronic intestinal inflammation. Currently, there are still a lack of natural and low-toxicity therapeutic approaches for UC. Emerging research has underscored the potential of plant-derived exosomes for UC treatment. In this study, it is found that Centella Asiatica-derived exosomes (CAEs) have anti-colitis effects. CAEs selectively accumulate in inflamed colons, enabling targeted delivery of RNA cargos. MicroRNA (miRNA)-enriched CAEs not only mitigate inflammation but also facilitate the reconstitution of a balanced gut microbiota, characterized by a deceased abundance of pathogenic bacteria (e.g., Salmonella enterica ), as well as regulating 880 serum metabolites. Furthermore, the results indicate the remarkable impact of specific miRNAs from CAEs, such as aof-miR396b and fve-miR396c-3p , on Peak1 target. CAEs attenuate inflammatory responses and enhance the functions of immune cells in the intestinal milieu. Importantly, the safety profile of CAEs is exemplary, with no discernible adverse effects observed in both in vitro and in vivo assays. This study posits plant-derived exosomes as a potent, targeted, and safe therapeutic modality for UC, representing an alternative to conventional treatments by leveraging the inherent bioactive properties of botanically derived nanovesicles and the cross-kingdom regulatory potential of plant-derived miRNAs on mammalian genes.
研究领域:
免疫学
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分子生物学
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转化医学
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炎症性肠病
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纳米医学
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微生物组学
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药理学
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植物生物技术
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2017
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IF 7.78
Yiyi Tao,Dan Yin,Mingchao Jin,Jie Fang,Tao Dai,Yi Li,Yuxia Li,Qinli Pu,Guoming Xie
An electrochemical microRNA (miRNA) analysis platform by combining double-loop hairpin probe (DHP) and doxorubicin-loaded gold nanoparticles ( [email protected] ) for ultrasensitive miRNA detection is proposed. Firstly, we here report a DHP that is simultaneously engineered to incorporate a miRNA recognition sequence, an output segment and output's complementary fragment. The important aspect of this hairpin probe is that it would not be degraded by duplex specific nuclease (DSN) and circumvents elaborately chemical modification disadvantages encountered by classic molecular beacon . For the DHP-based DSN signal amplification system, DHP hybridizes with target miRNA to form DNA-miRNA heteroduplexes , and the DSN can hydrolyze the DNA in the heteroduplexes structure selectively, while released target miRNA strand can initiate another cycle resulting in a significant signal amplification and the accumulated output segments could be responsible for strand displacement on the electrode directly. Furthermore, a great deal of doxorubicin (Dox) are loaded on the gold nanoparticles (AuNPs) to fabricate the [email protected] biocomposites that could magnify the electrochemical signal and enable the ultrasensitive analysis of miRNA. As a result, the miRNA was capable of being detected in a limit of 0.17 pM and other kinds of miRNA were discriminated facilely by this method. The described DHP as a toolbox and the nano-biocomposites as a novel signal material would not only promote the design of electrochemical biosensors but also open a good way to promote the establishment of test method in malignant tumors.
研究领域:
生物化学
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分子生物学
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纳米医学
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DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
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2021
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IF 5.373
Ya Zhang,Ning Li,Wenhao Ma,Mei Yang,Changjun Hou,Xiaogang Luo,Danqun Huo
MicroRNA exhibits different levels of expression in cancer and can affect the transformation, metastasis, and carcinogenesis of the cancer cell. Herein, we developed a novel kind of electrochemical microRNA biosensor based on two-dimensional nanomaterial of antimonene nano-flakes (AMNFs) and carbon quantum dots (CQDs) which were used as substrating to cadmium ion (Cd 2+ ) for specific detection of breast cancer-relevant biomarker-microRNA-21. Compared to graphene, the first principle energetic calculation shows that the AMNFs have completely a stronger force interaction with single strand (ssRNA), due to the antimonene has a more delocalized 5 s/5p orbital. After the addition of complementary microRNA, due to the low adsorption affinity of double-stranded RNA (dsRNA) to antimonene, the hybridized target is easy to desorb from the antimonene interface, and the oxidation peak of metal ions is significantly reduced. Results showed the microRNA-21 concentration can be detected from 100 aM to 1 nM, the limit of detection as low as 21 aM toward microRNA-21, which is 3 times lower than those of the established microRNA biosensors. The unique combination of not be attempted before existing sensing material which has special adsorption properties represents an approach to the detection of breast cancer. And it provides a promising method for early diagnosis, monitoring, and staging of breast cancer.
研究领域:
生物化学
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纳米技术
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癌症研究
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分子生物学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 5
·1-10
·
IF 5.833
Wang Dongwei,Wang Jiasheng
The potential of functionalized polypyrrole nanowires (PPyNWs) are demonstrated as a platform for lable-free miRNA detection using electrochemical impedance spectroscopy (EIS). MicroRNAs (miRNAs) detection methods and sensors are mainly challenged by very low concentrations in physiological samples and high similarity among family members. Herein, a sensitive and selective miRNA biosensor was constructed based on electrochemically synthesized PPyNWs, which were functionalized with polyamidoamine dendrimer (PAMAM) by an electro-oxidation method. The prepared PPyNWs/PAMAM hybrid combines the excellent electrical conductivity of conducting polymer PPyNWs with high surface to volume ratio of PAMAM. DNA probes were immobilized onto the PPyNWs/PAMAM hybrid for the construction of the miRNA biosensor. Using the sensitive EIS technique to monitor DNA/miRNA hybridization, the developed biosensor demonstrated excellent sensing performances, such as wide linear range (10 −14 M-10 −8 M) and low detection limit (0.34 × 10 −14 M). Even more encouraging, the response sensitivity of the biosensor was 3.12 times higher than that of the bulk PPy-modified sensor, which proved that the microstructure of the PPy nanowires array can greatly improve the performance of the biosensor. Graphical abstract
An ultrasensitive and selective miRNA biosensor was constructed based on electrochemically synthesized polypyrrole nanowires array (PPyNWs), which were functionalized with polyamidoamine dendrimer (PAMAM) by an electro-oxidation method.
研究领域:
生物工程
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分析化学
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纳米技术
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分子生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Keying Zhang,Na Zhang,Yilin Wu,Wen Zhang,Fajun Li,Yizhong Shen
MicroRNA (miRNA) upconversion nanoprobes using organic dyes as acceptors have attracted intensive attention in early cancer diagnosis, but still remains a large challenge from the unsatisfactory luminescence resonance energy transfer (LRET) efficiency. Herein, we proposed to integrate the confined emitters effect and target cycling amplification strategy into a single matrix for establishing the upconversion nanoamplicon with high LRET efficiency, allowing for accurately reporting the levels of miRNA originated from cancer cells. As a proof-of-concept, the emitting ions were confined in the shell of the core-shell upconversion nanoparticles (UCNPs), enabling an enough close distance to its surface energy acceptors. The tentacle DNAs (P-tDNA-M), phosphorylated and methylated at both ends, were immobilized onto the UCNPs surfaces for effectively capturing hairpin DNA1 labeled with BHQ1 (BHQ1-HP1) to form BHQ1-UCNPs energy donor-acceptor pair, achieving ∼ 67.9 % of LRET efficiency. Target miRNA-21 could bind with HP1 to open the loop, causing BHQ1 away from UCNPs, thereby restoring the upconversion luminescence, allowing for high sensitively detecting miRNA-21 with a detection limit of 75.3 fM. Interestingly, this naoprobe exhibited good performances for monitoring miRNA-21 in human serum samples and evaluating the expression level of miRNA-21 in various cell lines. This proposed platform gives a new insight to design the efficient upconversion nanoprobes for precisely detecting low-expressed miRNA in complex biological samples.
研究领域:
生物医学工程
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分子诊断学
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纳米医学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
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IF 4.098
Jingcheng Cui,Qingxiang Guan,Han Lv,Kaifang Fu,Rao Fu,Zhenyu Feng,Feiyong Chen,Guiqin Zhang
Novel approaches are required to overcome the challenges associated with conventional microRNA (miRNA) detection methods and realize the early diagnosis of diseases. This work describes a novel label-free surface-enhanced Raman spectroscopy (SERS) method for the detection of the miRNA biomarkers for pneumoconiosis on a three-dimensional Au-coated ZnO nanorod array (Au-ZnO NRA). The Au-ZnO NRA substrate, which was fabricated via a modified seeding method combined with ion sputtering, provided a high enhancement factor and good spatial uniformity of the signal. With the Au-ZnO NRA, the SERS spectra of miRNAs were obtained in 30 s without labeling at room temperature. Density functional theory calculations were performed to understand the structural fingerprints of the miRNAs. Principal component analysis was carried out to identify the pneumoconiosis biomarkers based on their fingerprint SERS signals. Dual-logarithm linear relationships between the SERS intensity and the miRNA concentration were proposed for quantitative analysis. The label-free SERS method has limits of detection on the femtomolar level, which is much lower than the concentrations of the miRNA biomarkers for pneumoconiosis in lung fibroblasts.
研究领域:
纳米技术
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生物医学工程
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分析化学
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分子生物学
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DOI
PubMed
高影响因子
NEUROCHEMICAL RESEARCH
·
2019
·Vol. 44
·期 12
·2821-2831
·
IF 2.782
Zhai Ailing,Zhang Zeng,Kong Xiangjuan
Paeoniflorin (PF) has been reported to possess neuroprotective influences on cognitive dysfunction illness. In current research, we attempted to probe into the protective influences of PF against H 2 O 2 -induced damage and the underlying regulating mechanisms on hippocampal HT-22 cells. HT-22 cells were pretreated with PF, and then induced by H 2 O 2 . Afterwards, the influences of PF pretreatment were examined using CCK-8 assay, apoptosis assay, western blot and ROS assay, respectively. In addition, the expression of microRNA-135a (miR-135a) was analyzed and altered by qRT-PCR and cell transfection, respectively. After overexpression of miR-135a, the effects of miR-135a mimic on cell functions were detected again. Moreover, influences of H 2 O 2 , PF and miR-135a overexpression on JAK2/STAT3 and ERK1/2 signal pathways were further investigated. Further experiments verified that PF pretreatment alleviated H 2 O 2 -induced oxidative stress through increasing cell viability, inhibiting cell apoptosis, reducing ROS generation and activating JAK2/STAT3 and ERK1/2 pathways. Besides, expression of miR-135a was declined by PF pretreatment. Whereas, miR-135a mimic abrogated the protective effects triggered by PF pretreatment. These results indicated that PF can alleviate H 2 O 2 -induced oxidative stress by down-regulation of miR-135a via activation of JAK2/STAT3 and ERK1/2 pathways.
研究领域:
药理学
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神经科学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2015
·Vol. 19
·期 5
·1301-1309
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IF 2.446
Li Bingchen,Li Xue,Wang Mo,Yang Zhiqing,Yin Huanshun,Ai Shiyun
A selective and sensitive photoelectrochemical assay was proposed for microRNA detection based on the isothermal cycle hybridization amplification of microRNA-21 that originated from the specific cleavage activity of duplex-specific nuclease (DSN) toward a DNA probe in DNA-RNA double helix and in situ enzymatic production of electron donor of ascorbic acid. After the biotin-functionalized DNA probe hybridized with complementary target microRNA-21, the hybridized DNA probe could be cleaved by DSN and microRNA-21 was released back to the incubation solution. The released microRNA could be further hybridized with the remaining single-strand DNA probe; thus, the isothermal cycle hybridization amplification would be formed. As a result, little avidin-alkaline phosphatase conjugate could be captured due to the release of biotin originating from the DSN cleavage. The distinct photocurrent change between a control biosensor and the DSN cleavage biosensor achieved label-free microRNA-21 detection with the linear range from 1 to 500 fM. The fabricated biosensor showed high detection selectivity even for one-base mismatched sequence. The attempt was carried out to directly assay the expression level change of microRNA-21 in total RNA extracted from chicken fibroblast cells infected with subgroup J avian leukosis virus.
研究领域:
生物传感器
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分子生物学
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分析化学
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病毒学
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DOI
高影响因子
Molecular Therapy-Nucleic Acids
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2017
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IF 6.392
Yingting Yu,Yi Yao,Hao Yan,Rui Wang,Zhenming Zhang,Xiaodan Sun,Lingyun Zhao,Xiang Ao,Zhen Xie,Qiong Wu
Targeted therapy for cancer is a research area of great interest, and magnetic nanoparticles (MNPs) show great potential as targeted carriers for therapeutics. One important class of cancer biomarkers is microRNAs (miRNAs), which play a significant role in tumor initiation and progression. In this study, a cascade recognition system containing multiple plasmids, including a Tet activator, a lacI repressor gene driven by the TetOn promoter, and a reporter gene repressed by the lacI repressor and influenced by multiple endogenous miRNAs, was used to recognize cells that display miRNA signals that are characteristic of cancer. For this purpose, three types of signal miRNAs with high proliferation and metastasis abilities were chosen (miR-21, miR-145, and miR-9). The response of this system to the human breast cancer MCF-7 cell line was 3.2-fold higher than that to the human breast epithelial HBL100 cell line and almost 7.5-fold higher than that to human embryonic kidney HEK293T cells. In combination with polyethyleneimine-modified MNPs, this recognition system targeted the tumor location in situ in an animal model, and an ≃42% repression of tumor growth was achieved. Our study provides a new combination of magnetic nanocarrier and gene therapy based on miRNAs that are active in vivo , which has potential for use in future cancer therapies.
研究领域:
癌症研究
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纳米技术
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基因治疗
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Qi Wang,Wenyong Li,Juan Xia,Hai Wu,Lijun Wu,Jianguo Xu
The construction of DNA nanodevices is vital for disease diagnosis and biomedical study. Herein, we proposed a ternary DNA nanomachine for microRNA determination using a molecular beacon (MB) coupled Y shape probe (MB-YP). The MB-YP was a tripartite junction scaffold connected with MB-Y I , Y II , and Y III . The target miRNA firstly recognizes MB-Y I , activating the MB-Y I -DNA nanomachine to produce endogenous primer I (EP I ) responsible for the activation of Y II -DNA nanomachine. The Y II -DNA nanomachine then allows the production of numerous EP II , which in turn activates the Y III -DNA nanomachine to produce EP III for re-activating MB-Y I -DNA nanomachine. The stepwise operated MB-Y I -DNA nanomachine, Y II -DNA nanomachine, and Y III -DNA nanomachine constituted the ternary DNA nanomachine and the repeated use of EP I , EP II , and EP III permits it an all-in amplification. These features lead to the opening of MB-Y I for emitting strong fluorescence. As low as 1 fM miRNA can thus be quantitatively detected and the linear response of the miRNA is from 1 fM to 100 pM (six orders of magnitude). Moreover, the nanomachine is specific to distinguish target miRNA from one-base mutant, inserted, and deleted target analogs. Its practicability was demonstrated by confirming miRNA-21 is overexpressed in clinical human serums from lung cancer patients than healthy donors.
研究领域:
生物医学工程
·
分子生物学
·
纳米技术
·
癌症诊断
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Mengnan Yang,Zhenqiang Wang,Tao Ding,Jia Tang,Xiyue Xie,Yuxin Xing,Lu Wang,Jixi Zhang,Kaiyong Cai
Nanoquencher-based biosensors have emerged as powerful tools for the detection of tumor markers, where challenges in efficiently docking the π-electron interaction interface toward nucleic acid probes containing π-electron-rich units of bases and fluorescent dyes still remain. Herein, we present hybrid polydopamine/polypyrrole nanosheets (PDA-PPy-NS) with π electron coupling and ultranarrow band gap (0.29 eV) by interfacial engineering of polymer hybrids at the nanoscale. PDA-PPy-NS were first prepared through oxidant-induced polymerization of pyrrole on PDA nanosheets. By utilizing fluorescent-dye-labeled single-stranded DNA as a probe, the hybrid nanoquencher showed ultrahigh fluorescence quenching ability, i.e., a Cy5-ssDNA/nanoquencher mass ratio of 36.9 under the complete quenching condition, which is comparable to that of graphene oxide. It was demonstrated that the energy level coupling of nanosheets and nucleic acid dye (Cy5) was the key factor contributing to the efficient photoinduced electron transfer (PET). Subsequently, the nanoquencher/DNA probe was proved to possess superior sensitivity and selectivity for efficient and reliable detection of miRNA-21 with a detection limit of 23.1 pM. Our work proves that the π-electron-rich biosensor interface can significantly enhance the PET efficiency, providing a theoretical basis for developing novel high-performance sensors.
研究领域:
材料科学
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纳米技术
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生物医学工程
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分析化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）