Food Bioscience
·
2026
·
IF 6.2
Luyuqi Yi,Yuting Wang,Yu Wang,Jiawen Lu,Shiyu Chen,Qiang Yu,Jianhua Xie,Yi Chen
Co-exposure to AA and OTA disrupts the intestinal mucus barrier, with reduced claudin-1 and MUC2 expression, goblet cell loss, and downregulated GALNT2/COSMC. • Co-exposure-induced redox imbalance is accompanied by decreased PNA-detectable core 1 (Galβ1-3GalNAc) and downregulated glycosyltransferases. • Ferulic acid alleviates intestinal mucus barrier injury, accompanied by improved redox status and O-glycan-related markers. : The small intestine is highly susceptible to foodborne contaminants including acrylamide (AA) and ochratoxin A (OTA). Ferulic acid (FA), a natural bioactive phytochemical, exhibits great potential in preserving intestinal integrity. Here, we report that FA alleviates intestinal mucus barrier injury induced by AA/OTA co-exposure, and this protection is associated with modulation of oxidative stress homeostasis and O-glycan-related alterations—suggesting a previously underexplored association. In vivo , co-exposure of C57BL/6 mice to AA and OTA caused severe intestinal barrier dysfunction, as evidenced by increased intestinal permeability, goblet cell loss, downregulated expression of tight junction proteins and Mucin 2 (MUC2), excessive oxidative stress, and decreased levels of the O-glycan core 1 structure (Galβ1-3GalNAc). Notably, FA supplementation was associated with significant attenuation of these alterations. In the Caco-2/HT29-MTX co-culture model, FA antagonized AA/OTA-induced cytotoxicity, restored transepithelial electrical resistance (TEER), reduced paracellular permeability, and markedly upregulated the levels of the tight junction protein claudin-1 and the mucin MUC2. FA treatment was accompanied by mitigation of oxidative stress and increased expression of key glycosyltransferases, including GALNT2 and COSMC. Collectively, the coordinated changes in redox markers, GALNT2/COSMC expression, the PNA-detectable core 1 signal, and mucus-barrier indices suggest a coordinated association among redox imbalance, O-glycan-related alterations, and mucus-barrier dysfunction during AA/OTA-induced intestinal injury. These findings support further investigation of FA as a food-derived protective compound. Download: Download high-res image (188KB) Download: Download full-size image
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Xiang Yangjiayi,Liu Hongmei,Feng Jianan,Li Hanyin,Li Guanghui,Li Yan
Ochratoxin A (OTA) poses significant health risks and is prevalent in various plant-based foods, necessitating rapid and reliable on-site detection methods. In this study, a lateral flow aptasensor integrating magnetic solid-phase extraction (MSPE) with enzyme-catalyzed colorimetric signal transduction was developed for the rapid determination of OTA. To facilitate field deployment, a portable integrated MSPE device featuring adjustable rotation speed and duration, as well as automated magnetic separation, was designed to enable efficient sample pretreatment under on-site conditions. Polydopamine-coated magnetic beads were employed for MSPE due to their suitability for batch sample processing. Moreover, enzyme-catalyzed colorimetry was introduced as an alternative signal transduction strategy in lateral flow assays. The proposed aptasensor exhibited good specificity and satisfactory sensitivity, with a limit of detection of 0.30 ng/mL. Acceptable accuracy and precision were achieved, with recoveries of 91.1–97.2% and relative standard deviations below 15%. The method was further validated by analyzing commercially available food samples, with results showing good agreement with the standard HPLC method. This integrated strategy enables reliable on-site detection of OTA in complex food matrices by combining efficient magnetic sample pretreatment with enhanced enzyme-catalyzed colorimetric signal transduction. Graphical abstract
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Jiaojiao Zhou,Xuqin Lv,Yue Gui,Jiangling He,Fang Xie,Jie Cai
Ochratoxin A (OTA) is a powerful mycotoxin present in a variety of food products, and its detection is important for human health. Here, a fluorescent aptasensor is reported for sensitive OTA determination. Specifically, the surface of bio-inspired passion fruit-like dendritic mesoporous silica nanospheres-enriched quantum dots (MSNQs-apt) was first modified with the OTA aptamer as the recognition unit and fluorescence emitter, while the aptamer-complementary DNA (MNPs-cDNA) was linked with the magnetic nanoparticles (MNPs) as the separation element. In the range of 2.56 pg/mL to 8 ng/mL, the proposed aptasensor exhibited satisfactory linearity and a detection limit of 1.402 pg/mL. The developed aptasensor achieved recoveries of 90.98–103.20% and 94.33–107.57 % in red wine and wheat flour samples, respectively. By simply replacing the aptamer, this aptasensor can be easily extended to detection of other analytes, suggesting its potential as a universal detection platform for mycotoxins in food products.
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Foods
·
2023
·Vol. 12
·期 4
·707
·
IF 5.2
Linzhi Li,Xiaofeng Wang,Jian Chen,Tianzeng Huang,Hongmei Cao,Xing Liu
In this study, an electrochemiluminescence (ECL) immunosensor based on nanobody heptamer and resonance energy transfer (RET) between g-C3N4(g-CN) and NU-1000(Zr) was proposed for ultrasensitive ochratoxin A (OTA) detection. First, OTA heptamer fusion protein was prepared by fusing OTA-specific nanometric (Nb28) with a c-terminal fragment of C4 binding protein (C4bpα) (Nb28-C4bpα). Then, Nb28-C4bpα heptamer with the high affinity used as a molecular recognition probe, of which plenty of binding sites were provided for OTA-Apt-NU-1000(Zr) nanocomposites, thereby improving the immunosensors’ sensitivity. In addition, the quantitative analysis of OTA can be achieved by using the signal quenching effect of NU-1000(Zr) on g-CN. As the concentration of OTA increases, the amount of OTA-Apt-NU-1000(Zr) fixed on the electrode surface decreases. RET between g-CN and NU-1000(Zr) is weakened leading to the increase of ECL signal. Thus, OTA content is indirectly proportional to ECL intensity. Based on the above principle, an ultra-sensitive and specific ECL immunosensor for OTA detection was constructed by using heptamer technology and RET between two nanomaterials, with a range from 0.1 pg/mL to 500 ng/mL, and the detection limit of only 33 fg/mL. The prepared ECL-RET immunosensor showed good performance and can be successfully used for the determination of OTA content in real coffee samples, suggesting that the nanobody polymerization strategy and the RET effect between NU-1000(Zr) and g-CN can provide an alternative for improving the sensitivity of important mycotoxin detection.Keywords:Nb28-C4bpα heptamer;electrochemiluminescence immunosensor;resonance energy transfer;ochratoxin A
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Xiaobo Zhang,Zhiru Li,Lili Yang,Bing Hu,Qiuyue Zheng,Jiang Man,Jijuan Cao
The sensitive and accurate detection of ochratoxin A (OTA) is crucial for public health due to its high toxicity. Herein, using Au nanoparticle (NP)-attached CdS/UiO-66-NH2 heterostructures as photoactive materials, a photoelectrochemical (PEC) aptasensor was presented for the ultrasensitive assay of OTA based on a competitive displacement reaction triggering the trans-cleavage ability of CRISPR/Cas12a. In this sensing strategy, methylene blue-labeled single-stranded DNA (MB-ssDNA) was immobilized on the Au NPs/CdS/UiO-66-NH2 electrode to accelerate the separation of the photogenerated carrier, thus producing a significantly increased PEC response. In the presence of OTA, it specifically bound with the aptamer (Apt) and resulted in the release of the activation chain, triggering the trans-cleavage characteristics of CRISPR/Cas12a. MB-ssDNA was cut randomly on the electrode surface to convert the PEC signal from the “on” to the “off” state, thereby achieving a quantitative and accurate detection of OTA. The CRISPR/Cas12a-derived PEC aptasensor exhibited excellent sensitivity and specificity, with a linear range from 100 to 50 ng/mL and a detection limit of 38 fg/mL. Overall, the proposed aptasensor could provide a rapid, accurate, and sensitive method for the determination of OTA in actual samples.
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JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 42
·期 13
·2272-2279
·
IF 2.516
Xia Yu,Shuoke Lai,Li Wang,Yiqiong Chen,Xucong Lin,Zenghong Xie
Developing a high-performance modification protocol is critical for efficiently fabricating affinity monolith. Herein, by using 2,4,6-trichloro-1,3,5-triazine as the linker, a simple triazine-bridged approach was proposed for efficiently fabricating aptamer-grafted polyhedral oligomeric silsesquioxane-polyethyleneimine affinity monolith with high specificity toward ochratoxin A. Experimental parameters, column characteristics and specificity performances of the resultant affinity monolith were investigated in detail. Under the optimal conditions, 2,4,6-trichloro-1,3,5-triazine was rapidly grafted on the polyamine matrix, and effectively applied to the subsequent bridge linkage of aptamers. It was simple and effective, which resulted in a significant decrease of modification time, excellent properties including the high coverage density of aptamer up to 1799 pmol/μL and sensitive detection of ochratoxin A as low as 10 pg/mL in beer samples. This protocol provides a facile approach for fabricating aptamer-grafted polyamine affinity monoliths with highly selective discrimination performance.
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BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Chengxi Zhu,Dong Liu,Yuye Li,Xiuli Shen,Shuai Ma,Yang Liu,Tianyan You
A novel ratiometric electrochemical aptasensor was developed for Ochratoxin A (OTA) detection based on the binding of methylene blue (MB) to DNA with a dual signal amplification strategy. The formation of dsDNA structures between ferrocene-labeled complementary DNA (Fc-cDNA), the OTA aptamer , and complementary helper DNA (hDNA) caused Fc away from the electrode, and allowed dsDNA to bind with a certain amount of MB. Here, a small oxidation current of Fc (I Fc ) and a large oxidation current of MB (I MB ) were obtained. In the presence of OTA, its specific recognition with the aptamer induced the release of aptamer and hDNA from the electrode and subsequently the formation of hairpin structure for cDNA, which caused Fc close to the electrode and a weaker binding ability with MB. Then, an increased I Fc and a decreased I MB were obtained. Based on this principle, OTA could be accurately quantified by measuring the ratiometric signal of I Fc /I MB . Herein, the dual signal amplification strategy of the introduction of hDNA and the binding with MB after the OTA recognition was exploited to amplify the response signal. The obtained aptasensor showed a linear detection range from 10 pg mL −1 to 10 ng mL −1 and a detection limit of 3.3 pg mL −1 . The aptasensor was successfully applied to determine OTA in wheat, and the results were validated through HPLC-MS. Furthermore, by changing the target aptamers, this strategy could be universally used for the determination of various mycotoxins , showing promising potential applications for mycotoxins monitoring in agricultural products and foods.
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ANALYST
·
2019
·Vol. 144
·期 19
·5866-5874
·
IF 4.019
Pengxiang Wang,Luyan Wang,Mei Ding,Meishan Pei,Wenjuan Guo
To enhance the sensitivity of an aptasensor, a novel strategy was designed to develop an electrochemical aptasensor based on poly(3,4-ethylenedioxy thiophene)–gold nanoflower (PEDOT–AuNF) composites supported on a three-dimensional graphene oxide sponge (GOS). GOS with a three-dimensional sponge-like porous structure, exhibiting excellent electrical conductivity and a large surface area, provided the first amplification of the electrochemical signal for ochratoxin A (OTA) detection. PEDOT–AuNFs, synthesized by an ionic liquid-assisted one-pot method, presented a peculiar hierarchical flower-like structure, a high electroactive surface area, and more binding sites for immobilizing the aptamer molecules by the Au–S bonds. When PEDOT–AuNFs were supported on the surface of GOS by the interaction of the π–π packing between PEDOT and graphene oxide, a synergistic effect was produced to provide the second amplification for the aptasensor. PEDOT–AuNFs/GOS provided an ultrasensitive detection technique by multiple signal amplification for the electrochemical sensing of OTA. Consequently, this strategy not only endowed the aptasensor with high sensitivity but also needed no complicated signal amplification. The electrochemical sensor was fabricated successfully on a glassy carbon electrode to detect OTA with a linear response in the range of 0.01–20 ng L−1 and a limit of detection of 4.9 pg L−1. Moreover, it displayed good specificity, reproducibility and stability. The utilization of the proposed aptasensor for the quantitative determination of OTA in wine indicates that it can find promising applications in detecting OTA and even other mycotoxins in foodstuffs.
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ANALYST
·
2019
·Vol. 144
·期 10
·3389-3397
·
IF 4.019
Jingfeng Wang,Yu Wang,Su Liu,Haiwang Wang,Xue Zhang,Xiaolei Song,Jinghua Yu,Jiadong Huang
DNA can be configured into unique high-order structures due to its significantly high programmability, such as a three-way junction-based structure (denoted Y-shaped DNA), for further applications. Herein, we report a label-free fluorescent signal-on biosensor based on the target-driven primer remodeling rolling circle amplification (RCA)-activated multisite-catalytic hairpin assembly (CHA) enabling the concurrent formation of Y-shaped DNA nanotorches (Y-DNTs) for ultrasensitive detection of ochratoxin A (OTA). Two kinds of masterfully-designed probes, termed Complex I and II, were pre-prepared by the combination of a circular template (CT) with an OTA aptamer (S1), a substrate probe (S2) and hairpin probe 1 (HP1), respectively. Target OTA specifically binds to Complex I, resulting in the release of the remnant element in S2 and successive remodeling into a mature primer for RCA by phi29 DNA polymerase, thus a usable primer-CT complex is produced, which actuates primary RCA. Then, numerous Complex II probes can anneal with the first-generation RCA product (RP) with multiple sites to activate the CHA process. With the participation of endonuclease IV (Endo IV) and phi29, HP1 as a pre-primer containing a tetrahydrofuran abasic site mimic (AP site) in Complex II is converted into a mature primer to initiate additional rounds of RCA. So, countless Y-DNTs are formed concurrently containing a G-quadruplex structure that enables the N-methylmesoporphyrin IX (NMM) to be embedded, generating remarkably strong fluorescence signals. The biosensor was demonstrated to enable rapid and accurate highly efficient and selective detection of OTA with an improved detection limit of as low as 0.0002 ng mL−1 and a widened dynamic range of over 4 orders of magnitude. Meanwhile, this method was proven to be capable of being used to analyze actual samples. Therefore, this proposed strategy may be established as a useful and practical platform for the ultrasensitive detection of mycotoxins in food safety testing.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 2
·1-7
·
IF 6.232
Wei Min,Xin Lingkun,Feng Shuo,Liu Yong
A complementary DNA (cDNA) was designed to simultaneously hybridize with the ochratoxin A (OTA) aptamer and the fumonisin B1 (FB1) aptamer to form a unique Y-shaped DNA structure and to achieve simultaneous detection. Gold nanorods (AuNRs) were used to immobilize thionine (Th), thiolated ferrocene (Fc), thiolated OTA aptamer (Apt1), and thiolated FB1 aptamer (Apt2), to form an amplified signal element and a recognition element. The Apt1-AuNRs-Th complex and the Apt2-AuNRs-Fc complex hybridize with cDNA to form a unique Y-DNA structure on a gold electrode. This produces two initial electrochemical signals [with 177 μΑ cm −2 near −0.2 V, and 3121 μΑ cm −2 near +0.46 V (vs. Ag/AgCl)] by differential pulse voltammetry. Upon addition of 0.1 ng mL −1 OTA and 0.1 ng mL −1 FB1, the aptamers bind the two toxins. This results in the release of Apt1-AuNRs-Th and Apt2-AuNRs-Fc, so the peak currents densities decrease to 115 μΑ cm −2 and 209 μΑ cm −2 . The assay allows simultaneous determination of OTA and FB1 in the 1.0 pg·mL −1 to 100 ng·mL −1 concentration ranges, with LODs of 0.47 and 0.26 pg·mL −1 . The assay is reproducible, stable and specific. It was applied to the determination of OTA and FB1 in spiked beer, with recoveries between 89.0% and 102.0%.
Schematic representation of OTA and FB 1 detection based on Apt 2 -AuNRs-Fc/Apt 1 -AuNRs-Th/cDNA/AuE. (AuNRs: Gold nanorods; Th: thionine; Fc: ferrocene; SH: thiol; BSA: Bovine serum albumin; cDNA: Complementary DNA; Apt 1 : Aptamer1; Apt 2 : Aptamer2; OTA: Ochratoxin A; FB 1 : Fumonisin B 1 ).
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FOOD AND CHEMICAL TOXICOLOGY
·
2024
·
IF 3.9
Jiawen Lu,Dan Su,Ying Yang,Mengni Shu,Yuting Wang,Xingtao Zhou,Qiang Yu,Chang Li,Jianhua Xie,Yi Chen
Mycotoxins and thermal processing hazards are common contaminants in various foods and cause severe problems in terms of food safety and health. Combined use of acrylamide (AA) and ochratoxin A (OTA) would result in more significant intestinal toxicity than either toxin alone, but the underlying mechanisms behind this poor outcome remain unclear. Herein, we established the co-culture system of Caco-2/HT29-MTX cells for simulating a real intestinal environment that is more sensitive to AA and OTA, and showed that the combination of AA and OTA could up-regulate permeability of the intestine via increasing LY permeabilization, and decreasing TEER, then induce oxidative stress imbalance (GSH, SOD, MDA, and ROS) and inflammatory system disorder (TNF-α, IL-1β, IL-10, and IL-6), thereby leading a rapid decline in cell viability. Western blot, PAS- and AB-staining revealed that AA and OTA showed a synergistic effect on the intestine mainly through the disruption of tight junctions (TJs) and a mucus layer. Furthermore, based on correlation analysis, oxidative stress was more relevant to the mucus layer and TJs. Therefore, our findings provide a better evaluation model and a potential mechanism for further determining or preventing the combined toxicity caused by AA and OTA.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Meixin Li,Pengfei Yu,Shanshan Li,Mati Ullah Khan,Shun Wang,Jiaqi Tian,Shuang Liu,Ling Kong,Zhiwei Chen
Aflatoxin B 1 (AFB 1 ) and ochratoxin A (OTA) are highly toxic mycotoxins prevalent in food and feed, posing severe threats to human and animal health. This work presents an innovative photoelectrochemical aptamer sensor for the detection of AFB 1 and OTA, leveraging a ternary BiOBr/Bi/BiOI heterojunction and Ag 2 S quantum dot-mediated signal amplification. The BiOBr/Bi/BiOI composite was synthesized via a one-step hydrothermal reduction using l -ascorbic acid (AA), exhibiting a high specific surface area to enhance biorecognition efficiency. In situ-assembled Ag 2 S quantum dots amplified photocurrent responses, while a single aptamer probe enabled specific dual-target recognition. Under optimized conditions, the sensor achieved exceptional sensitivity: linear ranges of 0.001–100 ng·mL −1 for both mycotoxins, with detection limits of 4.34 fg·mL −1 (AFB 1 ) and 13.54 fg·mL −1 (OTA). Validation in complex food matrices confirmed high accuracy and robustness. This platform not only provides a reliable method for mycotoxin monitoring but also establishes a versatile design paradigm for multiplexed detection of food contaminants.
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危险品化学品经营许可证（带存储）