INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Jundan Feng,Huimin Li,Yuzheng Lu,Rong Li,Artur Cavaco-paulo,Jiajia Fu
To enhance the enzymatic digestibility of polyethylene terephthalate (PET), which is highly oriented and crystallized, a polyethylene glycol (PEG) surfactant of varying molecular weights was utilized to improve the stability of mutant cutinase from Humicola insolens (HiC) and to increase the accessibility of the enzyme to the substrate. Leveraging the optimal conditions for HiC hydrolysis of PET, the introduction of 1 % w / v PEG significantly increased the yield of PET hydrolysis products. PEG600 was particularly effective, increasing the yield by 64.58 % compared to using HiC alone. Moreover, the mechanisms by which PEG600 and PEG6000 enhance enzyme digestion were extensively examined using circular dichroism and fluorescence spectroscopy. The results from CD and fluorescence analyses indicated that PEG alters the protein conformation, thereby affecting the catalytic effect of the enzyme. Moreover, PEG improved the affinity between HiC and PET by lowering the surface tension of the solution, substantially enhancing PET hydrolysis. This study suggests that PEG holds considerable promise as an enzyme protector, significantly aiding in the hydrophilic modification and degradation of PET in an environmentally friendly and sustainable manner.
研究领域:
环境科学
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生物技术
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材料科学
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酶学
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DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
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2013
·Vol. 20
·期 8
·1-6
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IF 2.019
Li Chunfang,Li Dongxiang,Qiu Chuanlong,Hou Wanguo
Amphiphilic dendritic macromolecules are suitable for the noncovalent encapsulation of guest molecules and could potentially be applied in drug-delivery systems. In this work, amphiphilic carbosilane dendrimers with peripheral poly(ethylene glycol) (PEG) units were synthesized using a hydrosilylation reaction, and their encapsulation properties were studied by fluorescence spectroscopy, using pyrene as the probe molecule. The results showed that the encapsulating capacity of the dendrimers increased as the number of PEG units increased, and that it also depended to some degree on the dendrimer generation.
研究领域:
药学
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材料科学
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DOI
高影响因子
Gels
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2022
·Vol. 8
·期 12
·819
·
IF 4.432
Pengfei Cui,Luping Ma,Pengju Jiang,Cheng Wang,Jianhao Wang
Currently, nucleic acid preparations have gained much attention due to their unique working principle and application value. However, as macromolecular drugs, nucleic acid preparations have complex construction and poor stability. The current methods to promote stability face problems such as high cost and inconvenient operatios. In this study, the hydrophilic pharmaceutical excipient PEG was used to gelate nucleic acid preparations to avoid the random movements of liquid particles. The results showed that PEG gelation significantly improved the stability of PEI25K−based and liposome−based nucleic acid preparations, compared with nucleic acid preparations without PEG gelation. After being stored at 4 °C for 3 days, non−PEG gelled nucleic acid preparations almost lost transfection activity, while PEGylated preparations still maintained high transfection efficiency. Fluorescence experiments showed that this effect was caused by inhibiting particle aggregation. The method described in this study was simple and effective, and the materials used had good biocompatibility. It is believed that this study will contribute to the better development of gene therapy drugs.Keywords:nucleic acid preparation;PEG gelation;stability
研究领域:
药剂学
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分子生物学
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DOI
PubMed
高影响因子
LANGMUIR
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2020
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IF 3.557
Junnan Yang,Wei Liu,Yan Sun,Xiaoyan Dong
Deposition of amyloid-β (Aβ) aggregates in the brain is a main pathological hallmark of Alzheimer’s disease (AD), so inhibition of Aβ aggregation has been considered as a promising strategy for AD prevention and treatment. Black phosphorus (BP) is a 2D nanomaterial with high biocompatibility and unique biodegradability, but its potential application in biomedicine suffers from the rapid degradability and unfunctionability. To overcome the drawbacks and broaden its application, we have herein designed an Aβ inhibitor (LK7)-coupled and polyethylene glycol (PEG)-stabilized BP-based nanosystem. The PEGylated-LK7-BP nanosheets (PEG-LK7@BP) not only exhibited a good stability but also demonstrated a significantly enhanced inhibitory potency on Aβ42 fibrillogenesis in comparison with its counterparts. This elaborately designed PEG-LK7@BP stopped the conformational transition and suppressed the fibrillization of Aβ42, so it could completely rescue cultured cells from the toxicity of Aβ42 (by increasing the cell viability from 72 to 100%) at 100 μg/mL. It is considered that PEG-LK7@BP could bind Aβ species by enhanced electrostatic and hydrophobic interactions and thus efficiently alleviated Aβ–Aβ interactions. Meanwhile, the coupled LK7 on the BP surface formed a high local concentration that enhanced the affinity between the nanosystem and Aβ species. Finally, PEG could improve the stability and dispersibility of the nanoplatform to make it show an increased inhibitory effect on the amyloid formation. Hence, this work proved that PEG-LK7@BP is a promising nanosystem for the development of amyloid inhibitors fighting against AD.
研究领域:
生物医学
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纳米材料科学
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神经退行性疾病研究
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产品关联
DOI
PubMed
高影响因子
OPTICS COMMUNICATIONS
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2023
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IF 2.4
Kaixin Ge,Renhao Ni,Pan Tao,Xiangfen Zhao,Yang Luo,Yabin Zhu,Baoan Song,Wei Zhang,Shixun Dai,Ning Zhang,Tiefeng Xu,Peiqing Zhang
The early detection of esophageal cancer is crucial for enhancing patient survival. Surface-enhanced Raman scattering (SERS), renowned for its precise detection capabilities of low-concentration molecules and organisms, holds immense promise for early diagnosis. However, the use of silver nanoparticles to enhance the Raman signal can disrupt the detection process. To address this problem in this study, we synthesized polyethylene glycol (PEG)-coated silver nanocubes (PEG-AgNCs) as stable, label-free, and low-toxicity nanoprobes for detecting esophageal cancer. AgNCs with sharp corners and edges are selected as the SERS substrate to increase the strength of the Raman signal. To minimize the toxicity of AgNCs, the surface is coated with PEG, which exhibits outstanding biocompatibility. The SERS effectiveness of PEG-AgNCs was evaluated using 4-mercaptobenzoic acid as a Raman probe molecule, and the toxicity of PEG-AgNCs at different concentrations was investigated using human keratinocytes (HaCaT) and human esophageal carcinoma cells (TE-1). Results showed that PEG-AgNCs can effectively reduce the toxicity of AgNCs while maintaining a strong SERS signal. Remarkable differences in Raman spectra were observed between HaCat and TE-1 cells within the spectral range of 588–768 cm −1 , indicating the potential of PEG-AgNCs in the early detection of esophageal cancer using Raman detection. Our study highlights the potential of developing low-toxicity SERS biosensors for biological and chemical analyses.
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产品关联
DOI
高影响因子
BIOMACROMOLECULES
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2015
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IF 5.75
Hao Yang,Chuan Zhang,Chang Li,Yong Liu,Yingli An,Rujiang Ma,Linqi Shi
Polymeric nanoparticles with glucose-responsiveness are of great interest in developing a self-regulated drug delivery system. In this work, glucose-responsive polymer vesicles were fabricated based on the complexation between a glucosamine (GA)-containing block copolymer PEG45-b-P(Asp-co-AspGA) and a phenylboronic acid (PBA)-containing block copolymer PEG114-b-P(Asp-co-AspPBA) with α-CD/PEG45 inclusion complex as the sacrificial template. The obtained polymer vesicles composed of cross-linked P(Asp-co-AspGA)/P(Asp-co-AspPBA) layer as wall and PEG chains as both inner and outer coronas. The vesicular morphology was observed by transmission electron microscopy (TEM), and the glucose-responsiveness was investigated by monitoring the variations of hydrodynamic diameter (Dh) and light scattering intensity (LSI) in the polymer vesicle solution with glucose using dynamic light scattering (DLS). Vancomycin as a model drug was encapsulated in the polymer vesicles and sugar-triggered drug release was carried out. This kind of polymer vesicle may be a promising candidate for glucose-responsive drug delivery.
研究领域:
生物材料
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药剂学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
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2014
·Vol. 131
·期 12
·
IF 1.64
Wenliang Li,Wenjing Zhang,Xiuyun Yang,Zhigang Xie,Xiabin Jing
A series of four-arm star block copolymers poly(ethylene glycol)-block-poly( d,l -lactide) (PEG-PDLLA) with different hydrophobic length were synthesized by ring-opening polymerization of lactide and characterized using 1 H NMR and gel permeation chromatography (GPC). These copolymers could self-assemble in aqueous solution to form the vesicle structure with controlled size and morphologies. Transmission electron microscopy (TEM) and DLS show the polymersomes are spherical with diameter of 70∼500 nm. The polymersomes made by direct hydration of copolymer thin films in water exhibit the controllable ability of encapsulating hemoglobin under mild condition. The hemoglobin content in the polymersomes could reach to 35 wt %. More importantly, the encapsulated hemoglobin keeps its own bioactivity and is capable of binding oxygen. This hemoglobin-encapsulated four-arm PEG-PLA polymersomes could have the potential to be applied as an artificial oxygen carrier for transfusion. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131 , 40433.
研究领域:
生物材料
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医学工程
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产品关联
DOI
高影响因子
International Journal of Electrochemical Science
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2023
·
IF 1.5
Jiheng Ding,Wanjun Peng,Hongran Zhao,Haibin Yu
Aniline tetramer grafted polyethylene glycol methyl ether (AT-PEG) was obtained from aniline tetramer (AT), polyethylene glycol (PEG) and toluene-2,4-diisocyanate (TDI). Then the structure of AT-PEG was characterized by fourier infrared spectroscopy (FT-IR) and nuclear magnetic resonance ( 1 H NMR). The corrosion inhibition performance of AT-PEG for Q235 carbon steel in 1.0 M HCl solution has been investigated using static weight loss, electrochemical impendence spectroscopy (EIS) and polarization curves, respectively. The results proved that the corrosion inhibition efficiency increased with the increase of AT-PEG, and the corrosion inhibition efficiency of hydrochloric acid solution reached its maximum of 92.50% in the concentration of 100 mg/L. Here, the results of static weight loss, electrochemical impendence spectroscopy (EIS) and polarization curves were in good agreement with each other.
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2023
·
IF 6
Miaomiao Ma,Yuhong Qi,Zhanping Zhang
Polyethylene glycol (PEG) is widely utilized in marine antifouling materials; thus, the fundamental understanding of PEG characteristics in seawater is indispensable. Herein, a “membrane-seawater” separation chamber model is designed, and the molecular dynamics (MD) method is used to simulate the swelling process of PEG membranes in seawater; two characteristic lengths are developed from the concept of Gibbs surface to illustrate the relationship between seawater ions and PEG membranes; the amount of bound water in the hydration shell of PEG is calculated by experimental method. It is concluded that the water content of the PEG membrane in seawater is not much different from that of swelling in water; only the swelling rate changes; the seawater ions accelerate the swelling of the PEG membrane, but most gathered in the outer layer of PEG membrane; Ions change the distribution structure of the hydration layer, and the bound water of PEG decreases under the action of seawater ions. Moreover, the structure of the PEG chain in seawater has a change of “helix-ring”. The study of PEG in seawater will provide an effective supplement and theoretical basis for the modification design of PEG coating.
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Jiawei Lu,Yi Chen,Meng Ding,Xiaokun Fan,Jiawei Hu,Yihua Chen,Jie Li,Zhihan Li,Wenyong Liu
In order to develop better wound dressings, a novel chitosan hydrogel (Cn-Nm gel) was designed and fabricated by using aldehyde-4-arm polyethylene glycol (4r-PEG-CHO) to crosslink the chitosan dissolved in alkaline solution, amino-4-arm polyethylene glycol (4r-PEG-NH 2 ) was chosen as the additive simultaneously. The special dissolution technique and macromolecular crosslinking structure endows the Cn-Nm gels with better performance than that of gels prepared by acid dissolving method with micromolecule crosslinker. First, Cn-Nm gels own strong toughness with 500 kPa tensile strength and 1000% elongation, about 400% swelling ratio and fast water absorption rate. Second, about 300 kPa adhesive strength and strippability between the gels and skin is achieved. More importantly, Cn-Nm gels show nearly 100% antibacterial rate towards Escherichia coli and Staphylococcus aureus . Excellent biocompatibility is also proved by the mouse fibroblasts tests. All of the performance makes this developed chitosan-based gel be the potential candidate as a wound dressing.
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产品关联
DOI
PubMed
高影响因子
CANCER LETTERS
·
2023
·
IF 9.7
Boning Niu,Yuehuang Wu,Min Zhou,Ruimiao Lin,Pengjin Ge,Xiaohui Chen,Hu Zhou,Xiaokun Zhang,Jingjing Xie
Antibody-coated nanoparticles have been reported to have the extremely low delivery efficiency in solid tumors in preclinical trials. Though aptamers were considered to be superior over antibodies in cancer theranostics, whether PEGylated aptamer nanoparticles are better than antibody nanoparticles in improving delivery specificity and penetration efficiency of chemotherapeutics is still unknown. Here, we conjugate celastrol , a natural product with anti-tumor effect, onto PEGylated EpCAM aptamer or antibody dendrimers to obtain two nanoconjugates , and for the first time, conduct a comprehensive study to compare their performance in delivery specificity, intratumoral penetration ability and therapeutic outcomes. Our results showed that compared to antibody counterparts, PEGylated aptamer nanoconjugates exhibited the enhanced accumulation and retention specificities at tumor sites and the stronger intratumoral penetration capabilities by reducing the macrophage reservoir effects in solid tumors . When delivered celastrol to a colorectal xenograft tumor mice model by PEGylated aptamer dendrimers , 20 % of enhanced therapeutic efficiency was achieved compared to that by antibody-modified ones. Moreover, celastrol at 2 mg/kg delivered by PEGylated aptamer dendrimers showed the prominent anticancer efficiency (nearly 92 %) but without obvious side effects. These data firstly provide the proof-of-concept implementation that PEGylated aptamer nanoconjugates will display the great potential in the effective and safe cancer treatment with regard to the superiority over antibody ones in penetration abilities.
研究领域:
药理学
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肿瘤学
·
纳米医学
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DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2016
·
IF 3.485
Changyu He,Qinglai Yang,Lianjiang Tan,Bingya Liu,Zhenggang Zhu,Bing Gong,Yu-Mei Shen,Zhifeng Shao
The need for smart materials in the area of biotechnology has accelerated the development of stimuli-responsive copolymer micelles. Here, we reported a novel dual-stimuli-responsive block copolymer PEG-DMTK-SS-PLA with both dimethyl thioketal (DMTK) and disulfide linkage incorporated into the backbone, capable of triggering fast drug release properties upon both oxidative (H 2 O 2 ) and reductive (GSH) environment. The CMC values of these copolymer micelles ranging from 0.051 to 0.087 mg mL −1 , the average diameters are from 34 nm to 55 nm and 65 nm to 198 nm for blank and DOX-loaded micelles respectively. MTT assay conducted in NIH 3T3 cells showed the low cytotoxicity of these micelles even when the concentration reached up to 500 μg/mL. Considering tumor microenvironment’s diverse in kinds of tumor cells, fluorescence microscopy, flow cytometry and MTT activity analysis were conducted in several cancer cells (e.g., cervix, lung, gastric, and colon cancer cells), and further confirmed that the dual responsive PEG-DMTK-SS-PLA micelles were degraded much faster than that of non-responsive PEG-PLA and single responsive PEG-SS-PLA and PEG-DMTK-PLA micelles. These results indicate that the dual-responsive micelles are promising for efficient anticancer drug delivery.
研究领域:
生物技术
·
药剂学
·
肿瘤学
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DOI
高影响因子
危险品化学品经营许可证（带存储）