ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Feng Liu,Yuecheng Cui,Lei Wang,Hongwei Wang,Yuqi Yuan,Jingjing Pan,Hong Chen,Lin Yuan
It is important to effectively maintain and modulate the bioactivity of protein-nanoparticle conjugates for their further and intensive applications. The strategies of controlling protein activity via “tailor-made surfaces” still have some limitations, such as the difficulties in further modulation of the bioactivity and the proteolysis by some proteases. Thus, it is essential to establish a responsive protein-nanoparticle conjugate system to realize not only controllable modulations of protein activity in the conjugates by incorporating sensitivity to environmental cues but also high resistance to proteases. In the work reported here, Escherichia coli (E. coli) inorganic pyrophosphatase (PPase) and poly(N-isopropylacrylamide) (pNIPAM) were both fabricated onto gold nanoparticles (AuNPs), forming AuNP-PPase-pNIPAM conjugates. The bioactivity-modulating capability of the conjugates with changes in temperature was systematically investigated by varying the molecular weight of pNIPAM, the PPase/pNIPAM molar ratio on AuNP, and the orientation of the proteins. Under proper conditions, the activity of the conjugate at 45 °C was approximately 270% of that at 25 °C. In the presence of trypsin digestion, much less conjugate activity than protein activity was lost. These findings indicate that the fabrication of AuNP-protein-pNIPAM conjugates can both modulate protein activity on a large scale and show much higher resistance to protease digestion, exhibiting great potential in targeted delivery, controllable biocatalysis, and molecular/cellular recognition.
研究领域:
纳米生物技术
·
生物化学
·
材料科学
·
生物催化
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 46
·24263-24271
·
IF 3.708
Yong Liu,Wenlong Li,Lei Hou,Peiyi Wu
A thermosensitive hyperbranched polyethylenimine (HPEI) partially substituted with N-isopropylacrylamide (NIPAM) monomer (HPEI-pNIPAM) was obtained and its thermodynamic properties were compared with the previously reported fully substituted hyperbranched HPEI-NIPAM polymer using calorimetric and turbidity measurements and one- and two-dimensional correlation infrared spectroscopy. With fewer NIPAM units embedded in the interior of the HPEI backbone, more hydrogen bonds between CO and D2O in the HPEI-pNIPAM polymer could be transformed into CO/D–N bonds and fewer CO related intermediates were formed during heating. During both the heating and cooling of HPEI-pNIPAM, collapse and restoration of the branched backbone occurred first, followed by the formation and debonding of self-associated CO/D–N hydrogen bonds in the same process; all of these processes might be due to a lower conformational confinement effect in the interior backbone leading to the formation of weaker hydrophobic–hydrophobic interactions. The residual NH groups in the hyperbranched thermosensitive HPEI-pNIPAM polymer could be used to bind to graphene oxide by the reaction of NH with epoxyl and carboxyl groups; it could also serve as a blocking reagent to prevent the aggregation of graphene during the preparation process. The new thermosensitive graphene composite obtained has a good potential for use in various biomedical or biosensor applications and provides opportunities for other similar hyperbranched polymers to be developed with responsive effects to multi-stimuli.
研究领域:
高分子化学
·
材料科学
·
生物医学工程
·
纳米技术
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产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Chunfang Qin,Hao Wang,Hongyan Cui,Yue Wang,Ming Zhang,Haidong Li,Yuchen Liu,Jingyun Wang,Qixian Chen,Yan Zhao
To overcome the biological barriers in the journey of systemic gene delivery, a multifaceted genomic synthetic nanomedicine was elaborated and strategically equipped with a multiple of intriguing responsiveness. Particularly, core–shell plasmid DNA condensates were created based on polyionic complexation with block copolymer of polyethylene glycol (PEG)-polylysine (PLys), namely, the nanoscaled PLys&pDNA nanoparticle tethered with the biocompatible PEG surroundings. Furthermore, redox-reversible disulfide crosslinking was introduced into PLys&pDNA nanoparticle to accomplish adequate structural stabilities, and thermal-responsive polypropylacrylamide (PNIPAM) was introduced as the secondary intermediate surroundings onto the pre-formulated PLys&pDNA nanoparticle with the aim of preventing the potential enzymatic degradation from the environmental nucleases. Hence, hundreds of times prolonged survival and retention was determined in pertinent to the blood circulation properties. Additionally, the installation of a guide ligand at the distal end of PEG segments was proposed to encourage selective tumor uptake. A linear peptide of GPLGVRG, which is selectively susceptible to digestion by the tumor-enriched matrix metalloproteinase 2 (MMP-2), was used as the linkage between the shell and core. This peptide has been shown to detach the bio-inert PEGylation, resulting in further facilitated cell endocytosis and intracellular trafficking activities. Hence, the precisely defined synthetic nanomedicine, which exhibits desirable characteristics, efficient expression of the therapeutic gene in the affected cells, and contributed to potent therapeutic efficacy in systemic treatment of intractable tumors by encapsulating the anti-angiogenic gene.
研究领域:
纳米技术
·
药学
·
癌症研究
·
基因治疗
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Tianzong Jiang,Shili Gai,Yanqi Yin,Zewei Sun,Bingchen Zhou,Yubo Zhao,He Ding,Anees Ahmad Ansari,Piaoping Yang
Inspired by the natural stimulus–response of plants and animals, a range of soft equipment has been extensively developed and used in intelligent human–computer interaction, editable remote drives, and machine learning. Poly(N-isopropyl acrylamide) (PNIPAM) hydrogels have gained popularity thanks to their low phase transition temperature and excellent flexibility. However, the PNIPAM hydrogels suffer from poor mechanical properties, low electrical conductivity, and lack of self-responsiveness. Herein, a bilayer light/thermal cascaded-driven anisotropic poly acrylamide/poly(N-isopropylacrylamide-co-acrylamide)-polyvinyl alcohol-MXene (P/PP-M) actuator was prepared by a simple two-step polymerization method. The unique bionic light/thermal response mechanism enables rapid conversion of energy forms and the construction of remote editable actuators. Concretely, the mechanical properties of hydrogels (strain: 1014 %, stress: 94 KPa) have been greatly improved by copolymerizing MXene nanosheets and multiplex hydrogels through two-by-two interpenetrating molecular chains. MXene nanosheets formed dynamic networks conferring the hydrogel system with high conductivity and light/thermal conversion efficiency. The P/PP-M soft actuator features high sensitivity (Gauge factor = 3.62), fast response time (400 ms), and cycling durability (>500 cycles). Finally, the energy transfer mechanism from light to thermal energy to kinetic energy was corroborated by preparing a series of bionic equipment. Manual clips were prepared on this basis, which enables the transportation of objects and realizes the real-time reflection of the operating status of remotely driven soft manual clips through changes in electrical signals. Thus, the proposed efficient editable remote-driven machine recognition soft equipment provides new insights for developing intelligent flexible robots and wearable smart devices.
研究领域:
材料科学
·
机器人技术
·
生物医学工程
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机械工程
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
DESALINATION
·
2020
·
IF 7.098
Xuan Zhang,Shu Xiong,Chu-Xuan Liu,Liang Shen,Song-Lin Wang,Wan-Zhong Lang,Yan Wang
Substrate modification is advantageous to ameliorate the internal concentration polarization (ICP) phenomenon encountered by most thin-film composite (TFC) membrane in forward osmosis (FO) process, therefore promoting FO implementation to a large extent. Optimization of the intrinsic parameters of substrate (e.g. porosity and tortuosity) can generally be achieved by incorporating hydrophilic or pore-containing materials into the dope solution before the substrate fabrication. In this work, polyvinylidene fluoride (PVDF) substrate with embedded thermal-response poly (N-isopropyl acrylamide) (PNIPAM) microgels is employed for the construction of TFC-FO membrane, where the reversible shrinkage of PNIPAM microgels brings about a lower tortuosity and a higher porosity of the substrate at higher operation temperature, thereby mitigating the ICP impact of the TFC-FO membranes significantly. Effects of PNIPAM content on the properties of the substrates and the resultant polyamide (PA) layer, as well as the corresponding performance of the TFC-FO membranes were comprehensively investigated. The benchmarking shows that, TFC membrane with PNIPAM-modified substrate exhibits remarkably improved water flux of 40.2 L·m −2 ·h −1 and a reduced reverse salt flux of 10.1 g·m −2 ·h −1 , with DI water as feed (50 °C) and 1 M NaCl as draw solution (ambient temperature), which suggests its great potential for treating feed solution with relatively high temperature in the practical applications, for instance, the dyestuff wastewater enrichment.
研究领域:
膜科学
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环境工程
·
水处理
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材料科学
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产品关联
DOI
高影响因子
Environmental Science-Nano
·
2022
·Vol. 9
·期 4
·1330-1340
·
IF 9.473
Shuo Deng,Dongsheng Wang,Zhiqiang Xiong,Siyu Zhang,Dengyu Li,Jin Zeng,Xuejiao Zhang,Qing Zhao
Water contamination by pathogenic bacteria is a major challenge to human health. The conventional disinfection methods produce disinfection by-products that may be carcinogenic, while the recently-developed nanotechnologies face the difficulties of low efficiency and rate, secondary pollution, and tedious processes. To solve these problems, we designed a thermo-responsive poly(N-isopropylacrylamide)-functionalized black phosphorus (named BP–PNIPAM) to capture and eliminate bacterial cells under the stimuli of near-infrared (NIR) irradiation. PNIPAM is covalently linked to the surface of BP nanosheets by aryl diazonium reaction and in situ atom transfer radical polymerization (ATRP). Under short-time NIR irradiation, bacterial cells are captured and co-precipitated with BP–PNIPAM due to the thermally induced hydrophilic-to-hydrophobic phase transition of PNIPAM. If the irradiation time is prolonged, the wrapped bacteria are disinfected due to hyperthermia. More than 80% of E. coli and S. aureus are captured by 100 μg mL−1 BP–PNIPAM within 5 min-NIR irradiation, while the elimination efficiency towards E. coli, S. aureus, and anti-ampicillin E. coli reaches 100% in the presence of 50 μg mL−1 BP–PNIPAM within 20 min-NIR irradiation. Moreover, BP–PNIPAM exhibits no obvious toxicity to Caco-2 cells up to 100 μg mL−1 within 24 h. Therefore, BP–PNIPAM offers an efficient, biocompatible, and convenient approach for the capture and sterilization of pathogenic bacteria in water.
研究领域:
材料科学
·
纳米技术
·
环境科学
·
生物医学工程
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xinhui Hou,Yifan Song,Yupeng Lv,Peiyao Wang,Kun Chen,Guiying Li,Lei Guo
Temperature-responsive nanomicelles with aggregation induced emission (AIE) property were prepared by the host–guest complexation of ferrocene functionalized tetraphenyl (TPE-Fc) and β-cyclodextrin-poly ( N -isopropylacrylamide) (β-CD-(PNIPAM) 7 ). The AIE chromophore TPE-Fc bound to the hydrophobic cavity of cyclodextrin serves as the core of micelles, and temperature sensitive PNIPAM serves as the shell to give the micelles good solubility. The size of the nanomicelles is about 100 nm. At the excitation wavelength of 340 nm, the strongest fluorescent emission peak was 421 nm. The introduction of cyclodextrin star polymer increased the fluorescence intensity of nanomicelles, thus improving the recognition of probe to Fe 3+ and Fe 2+ . The fluorescent probe can quickly detect Fe 3+ and Fe 2+ in water within 5 min even in the presence of various interfering ions. The detection limits of Fe 3+ and Fe 2+ were 1.04 μM and 0.78 μM, respectively in the range of 10–90 μM. The formation of complex between the probe and Fe 3+ /Fe 2+ was supported by Job’s plot. The probe was successfully applied to the detection of Fe 3+ and Fe 2+ in actual water sample with a good recovery. In addition, a possible sensing mechanism for the interaction of iron ions with amide bond groups of nanomicelles was proposed.
研究领域:
材料科学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2020
·
IF 7.489
Yaqian Cai,Qianyu Yu,Hanying Zhao
Self-assembly of proteins into well-organized proteinosomes has attracted great interest due to the potential medical and biological applications of the structures. Herein, a new concept of electrostatic assisted fabrication of proteinosomes is proposed. The self-assembly is performed by using multi-step dialysis approach, where negatively charged bovine serum albumin-poly( N -isopropylacrylamide) (BSA-PNIPAM) bioconjugate and positively charged enzyme (lysozyme or trypsin) are initially dissolved in phosphate buffer (PB) solution at a high salt concentration, and subsequently the protein solution is dialyzed against PB solutions at low salt concentrations, resulting in the formation of biofunctional proteinosomes. Transmission electron microscopy (TEM), cryo-TEM and light scattering results all demonstrate the formation of hollow structures. The wall of a proteinosome is composed of BSA and enzyme (lysozyme or trypsin), and PNIPAM chains of the bioconjugate are in the corona stabilizing the structure. In comparison with the native enzymes, the enzyme molecules in the assemblies basically retain their bioactivities. The proteinosomes formed by BSA-PNIPAM and lysozyme can be dissociated in the presence of trypsin, and those self-assembled by BSA-PNIPAM and trypsin are able to be self-hydrolyzed, resulting in the dissociation of the structures in aqueous solution. The size and morphology changes of the proteinosomes in the hydrolysis are studied.
研究领域:
生物技术
·
材料科学
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER BULLETIN
·
2019
·Vol. 77
·期 2
·963-974
·
IF 1.858
Yang Xu,Sun Zhaojie,Gao Jingru,Yang Chunhui,Tang Dongyan
Temperature-responsive films of poly( N -isopropylacrylamide) (PNIPAM) were facilely fabricated by one-step plasma-initiated polymerization and then functionalized by self-polymerization of dopamine on the surface. High retention of the monomer structure and temperature-responsive properties in PNIPAM films were confirmed. After plasma-initiated polymerization, PNIPAM films formed protrusions and ridges surfaces. Moreover, cross-linker of N , N ′-methylenebisacrylamide with different dosage was introduced into the systems to effectively modulate the roughness of PNIPAM films and to supply better adhesive surface. Furthermore, in cell culture, satisfactory survival rate of the attached Hela cells was obtained on PNIPAM films, and the cell viability was improved further on PNIPAM/PDA films. The results indicated that such films might be applicable in medical treatment and tissue engineering, due to their adjusted adhesion ability and less toxicity to cells.
研究领域:
材料科学
·
生物材料
·
细胞生物学
·
组织工程
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产品关联
DOI
高影响因子
LANGMUIR
·
2014
·
IF 4.384
Xiaolu Jia,Yuandu Hu,Ke Wang,Ruijing Liang,Jingyi Li,Jianying Wang,Jintao Zhu
We demonstrate a rapid and robust method to fabricate uniform core–shell photonic crystal (PC) microbeads by the microfluidic and centrifugation–redispersion technique. Colored crystalline colloidal arrays (CCAs) were first prepared through centrifugation–redispersion approach by self-assembly of polystyrene–poly(N-isopropylacrylamide) (PS–PNIPAm) core/shell nanoparticles (NPs). Different from the conventional NPs (e.g., charged PS or PNIPAm NPs), PS–PNIPAm NPs could easily self-assemble into well-ordered CCAs by only one purification step without laborious pretreatment (e.g., dialysis or ion exchange) or slow solvent-evaporation process. The CCAs is then encapsulated into a transparent polymer shell with functional groups (e.g., copolymer of ETPTA and butyl acrylate (BA)), triggering the formation of core–shell PC microbeads which can be used as optical encoding microcarriers. Importantly, this technique allows us to produce core–shell PC microbeads in a rapid and robust way, and the optical reflections of the PC microbeads appear highly stable to various external stimuli (e.g., temperature, pH value, and ionic strength) relying on the features of the CCAs core and protection of the polymer shell. Moreover, various probe biomolecules (e.g., proteins, antibodies, and so on) can be easily linked on the surface of the core–shell PC microbeads owing to the hydrophilic modification induced by the hydrolysis of BA on the microbead surface, enabling the multiplex biomolecular detection.
研究领域:
材料科学
·
光学
·
生物技术
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2023
·
IF 5
Xinmeng Shi,Ye Xietao,Linqing Huang,Liangyin Wei,Chen Miaoying,Ding Qu,Congyan Liu,Qin Chen,Yuping Liu,Yan Chen
Transcatheter arterial chemoembolization (TACE) is a minimally invasive procedure performed in interventional radiology to restrict a tumor's blood supply. Blood-vessel-embolic agents play key role on TACE. In the present work, we reported a Bletilla striata polysaccharide/poly (N-isopropylacrylamide) (BSP/PNIPAM) thermosensitive microemulsion gel loaded with magnetic icaritin (ICT) microemulsion (IS-MEs) as embolic agent (ISBP) for the first time. IS-MEs co-loaded with ICT and superparamagnetic iron oxide nanoparticles (SPIONs) were prepared by water titration method. The particle size of IS-MEs was 27.97 ± 0.25 nm, encapsulation efficiency was 97.18%, and presented a regular sphere under TEM. BSP/PNIPAM was prepared by free radical polymerization, which has good biocompatibility, excellent procoagulant function and dense microstructure. The lower critical solution temperature (LCST) of ISBP was 30 °C, which had excellent in vitro injection performance. The microscopic morphology of ISBP was loose and porous, the long-acting sustained-release time could reach 144 h, and the maximum ICT release was (48.6 ± 8.5) %. IPSBs with SPIONs exhibit desirable imaging properties and macrophage M1 polarization function. During the rabbit renal artery embolization, ISBP showed excellent distal vascular embolization performance and there was no obstruction or adhesion of the catheter. These results suggest that a novel in situ multifunctional traditional Chinese medicine thermosensitive microemulsion gel embolic agent has been developed with a potential application as a liquid embolic solution for TACE.
研究领域:
介入放射学
·
药学
·
纳米技术
·
传统中药
·
癌症治疗
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Yanai Chen,Limin Chang,Ziheng Zhang,Min Zhou,Yuanwei Gao,Yong Wang,Yanfang Liu,Jianglei Qin
Thermo-responsive hydrogels with biodegradability have great application in biomedical areas, while the Poly(N-isopropylacrylamide) (PNIPAM) based hydrogels exhibiting lower critical solution temperature (LCST) around body temperature have shown promising applications in diverse areas such as drug delivery, tissue engineering, and wound repairing. In this study, PNIPAM based thermo-responsive copolymer with ketone functional group was polymerized and the LCST was regulated to body temperature, then biodegradable self-healing hydrogels with injectability were fabricated by cross-linking the thermo-responsive copolymers with dopamine functionalized pectin hydrazide (PDAH). The hydrogel exhibited good mechanical strength, excellent biocompatibility and thermo-responsive sustained drug release process. The nano-graphene oxide (GO) was incorporated to design near-infrared (NIR) sensitive nanocomposite hydrogel to serve as photothermal enhanced wound dressing with vancomycin loading. The composite hydrogel also showed pH-responsive drug release behavior and the NIR irradiation also accelerated the release rate based on photothermal induced phase transition. More importantly, the nanocomposite hydrogel with vancomycin loading and photothermal property can promoted burn wound repairing rate by inhibiting the bacterial growth. As a result, this GO composite hydrogel (GO/hydrogel) could act as NIR triggered photo-thermal enhanced drug delivery platform for promoted wound repairing in the near future.
研究领域:
材料科学
·
生物医学工程
·
药学
·
组织工程
·
伤口愈合
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）