INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2018
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IF 3.141
Yan Li,Tao Li,Long-Qiang Xiao,Yue-Fei Zhang
The polycondensation of 2-pyrazolines is presented. The products are oligomers with the five-membered rings of the 2-pyrazoline as the backbone and the reaction is a polycondensation reaction by decomposition of ROH from the hydrogen atom in the −NH– group and the −OR in the ester group. Metal catalysts, reaction periods, and the addition amounts of the catalyst are investigated to optimize the reaction conditions. The products are down-/up-conversion fluorescence molecules that can significantly enhance cell imaging through the synchronized excitation of visible and near-infrared light and may be widely used in other optical fields.
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Jove-Journal of Visualized Experiments
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2018
·
IF 1.184
Yanlan Wang,Jiayu Liu,Haitao Pan,Jieyu Xing,Xiaoqiong Wu,Qing Li,Zhong Wang
This protocol describes the construction and functional studies of a bispecific antibody (bsAb), GPC3-S-Fab. bsAbs can recognize two different epitopes through their two different arms. bsAbs have been actively studied for their ability to directly recruit immune cells to kill tumor cells. Currently, the majority of bsAbs are produced in the form of recombinant proteins, either as Fc-containing bsAbs or as smaller bsAb derivatives without the Fc region. In this study, GPC3-S-Fab, an antibody fragment (Fab) based bispecific antibody, was designed by linking the Fab of anti-GPC3 antibody GC33 with an anti-CD16 single domain antibody. The GPC3-S-Fab can be expressed in Escherichia coli and purified by two affinity chromatographies. The purified GPC3-S-Fab can specifically bind to and kill GPC3 positive liver cancer cells by recruiting natural killer cells, suggesting a potential application of GPC3-S-Fab in liver cancer therapy.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2017
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IF 3.649
Wenxi Wang,Anna Shao,Shisen Feng,Min Ding,Gan Luo
S-layer proteins (Slps) are crystalline arrays of protein on bacterial cell surface layers. Owning to their capability to reassemble on the surface of lipid layers, Slps have been employed to modify liposomes for various profits. But the interaction information between Slps and positively charged liposomes are destitute, especially the gastrointestinal adhesion of Slps-coating liposomes is rarely reported. In the present work, the Slps extracted from Lactobacillus helveticus were reassembled on the surface of novel positively charged liposomes composed of soybean lecithin , Eudragit ® RL100 and cholesterol. The particle size and remarkable changes of Zeta potential with various Slps/lipid weight ratios were determined by dynamic light scattering and phase analysis light scattering. Significant difference in fluorescence dequenching percentage of liposomes decorated by ODA-FITC confirmed Slps self-reassemble on the surface of liposomes. A higher integrity of vesicular membrane after the addition of Triton X-100 solution demonstrated the stability enhancement of Slps-coating liposomes. Fourier transform infrared (FTIR) spectroscopy illustrated the interaction came from non-covalent bond. The mucoadhesion of Slps-coating liposomes was evaluated by the resident FITC-LP on the gastric and intestinal tract of mice at 7 h and 12 h after intragastrical administration, which proved that the Slps-coating improved the gastrointestinal adhesion significantly.
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CHEMICAL RESEARCH IN TOXICOLOGY
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2016
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IF 3.025
Hui Wang,Yukun Feng,Qian Wang,Xiucai Guo,Wenlin Huang,Ying Peng,Jiang Zheng
Benzbromarone (BBR) is a therapeutically useful uricosuric agent but can also cause acute liver injury. The hepatotoxicity of BBR is suggested to be associated with its metabolic activation. Our recent metabolic study demonstrated that BBR was metabolized to epoxide intermediate(s) by cytochrome P450 3A, and the intermediate(s) was reactive to N-acetylcysteine. The objectives of the present study were to determine the chemical identity of the interaction of protein with the epoxide intermediate(s) of BBR and to define the association of the protein modification with hepatotoxicity induced by BBR. Microsomal incubation study showed that the reactive intermediate(s) covalently modified microsomal protein at cysteine residues. Such adduction was also observed in hepatic protein obtained from liver of mice given BBR. The protein covalent binding occurred in time- and dose-dependent manners. Pretreatment with ketoconazole attenuated BBR-induced protein modification and hepatotoxicity, while pretreatment with dexamethasone or buthionine sulfoximine potentiated the protein adduction and hepatotoxicity induced by BBR. A good correlation was observed between BBR-induced hepatotoxicity and the epoxide-derived hepatic protein modification in mice. The present study provided in-depth mechanistic insight into BBR-induced hepatotoxicity.
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CLEAN-Soil Air Water
·
2016
·Vol. 44
·期 10
·1315-1322
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IF 1.716
Tao Chen,Zhangyun Liu,Jinting Yao,Hongyuan Hao,Feng Chen
Fenton-like degradation of three representative s -triazine herbicides, atrazine (Atz), ametryn (Amt), and prometon (Prt), was studied in this work. The effects of initial H 2 O 2 concentration as well as the Fe 3+ concentration on the degradation rate of the herbicides were investigated. The Fenton-like degradation of the s -triazine herbicides fitted well with pseudo first-order kinetics. LCMS-IT-TOF was used to analyze possible degradation intermediates for the three herbicides. Eleven, eleven, and twelve intermediates were identified for Atz, Amt, and Prt, respectively. According to the identified intermediates, the Fenton-like degradation pathways of Atz, Amt, and Prt were proposed, which were suggested to follow two main routes, alkylic oxidation and hydroxylation substitution. The enhanced electron-donating ability of the s -triazine herbicide benefits both degradation reaction routes. Therefore, Amt gives the highest reactivity, while Atz exhibits the highest resistance among three herbicides to the Fenton-like reaction.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
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IF 7.7
Que Bai,Fangfang Hu,Siyuan Gou,Qian Gao,Shihao Wang,Wenhui Zhang,Yanni Zhang,Tingli Lu
The damaged skin for some reasons is vulnerable to invasion by bacteria and other harmful microorganisms, leading to delay of the wound healing. In order to promote the infected wound healing, curcumin was loaded with chitosan-based hydrogel was formed through phenylborate ester bonding and its properties and effects on the S. aureus -infected wound healing was tested. It was found the hydrogel showed good antioxidation on the intracellular reactive oxygen species, inhibition on the growth of S. aureus , and acceleration the infected skin healing. The ablity of hydrogel due to its regulating inflammation, promoting angiogenesis and collagen synthesis in the wound site. This research work suggested that the developed multifunctional hydrogel might be a beneficial treatment for the infected wound healing.
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Journal of Materials Chemistry A
·
2024
·
IF 10.7
Xi Chen,Wei Zhou,Xu Fang,Cheng Li,Die Cong,Tie Yu,Wei-Qiao Deng,Chengcheng Liu
Photocatalytic CO2 reduction to syngas has recently garnered substantial interest due to its mild reaction conditions, high safety, and environmental sustainability. However, the efficiency of syngas production remains relatively low. In this study, we have developed metallosalen-based poly(ionic liquid)s (PILs) to enhance syngas production, achieving one of the highest production rates recorded to date. The Co(Salen)-PIL demonstrates a syngas production rate of 168.1 mmol g−1 h−1, including a CO production rate of 69.3 mmol g−1 h−1, which is more than eight times higher than that of the best previously reported organic materials. Mechanistic investigations reveal that pyridine ions facilitate electron transfer from the heptazine light-harvester to the Co(Salen) active site. This study lays the groundwork for developing high-performance catalysts for photocatalytic syngas production.
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Sensing and Bio-Sensing Research
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2024
·
IF 5.4
Weiran Mao,Xiaoyan Zhang,Yuanyuan Yin,Xiaohua Tang,Qingqing Jiang,Xia Chen,Xiaoliang Chen
This study proposes a novel sandwich-based ratio electrochemiluminescence (ECL) immunosensor for detecting monocyte chemotactic protein-1 (MCP-1), a marker of cardiovascular disease. We synthesized sulfur-doped palladium nanosheets (S-PdNS), which possess a stable structure and uniform morphology, and can quench signals generated by the S 2 O 8 2− /O 2 ECL system. Using glutaraldehyde (GA), we conjugated luminol to these nanosheets and combined them with a secondary antibody. This modification enabled the nanomaterial to produce a new luminol signal as an internal reference for detection while quenching the S 2 O 8 2− /O 2 ECL signal. To date, no studies have utilized both mechanisms for detection. Furthermore, the deposition of gold nanoparticles (AuNPs) on the surface of the glassy carbon electrode (GCE) allowed for increased antibody binding and improved electron transmission in the ECL system. When trace analytes are present, the S 2 O 8 2− /O 2 ECL signal decreases, and the luminol ECL signal increases. This setup allowed us to obtain two signals from one measurement and use the ratio to construct a standard curve, significantly reducing the possibility of misjudgment. Under optimal conditions, the linear detection range for MCP-1 is 10 –2.5 pg mL −1 to 10 3 pg mL −1 , with a detection limit of 1.6 fg mL −1 (S / N = 3). The immunosensor also exhibited excellent selectivity, repeatability, and stability, expanding the application of the dual luminescence mechanism and providing an effective method for early monitoring of cardiovascular disease.
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Joule
·
2024
·
IF 38.6
Boya Wang,Wanhai Zhou,Yanyan Zhang,Tengsheng Zhang,Xinran Li,Yutong Feng,Ruizheng Zhao,Wei Li,Ahmed Elzatahry,Yasser Hassan,Dongyuan Zhao,Dongliang Chao
Potassium-sulfur electrochemistry represents a compelling energy storage technology due to its cost-efficient chemicals and unparalleled capacity. However, achieving high sulfur redox utilization (SRU) remains a great challenge during K + storage due to K 2 S n kinetics inertia. Here, for the first time, we unveil an aqueous K + -S electrochemistry, leveraging boosted K 2 S n conversion kinetics in water. A stable two-electron charge transfer process is achieved via tuning K 2 S solubility. Spectroscopic evaluation and molecular dynamics simulations reveal a unique solid-liquid-solid conversion pathway (S ↔ K 2 S 4 ↔ K 2 S), which effectively avoids soluble K 2 S shuttling. Consequently, an unprecedented K + storage capacity of 1,619 mAh g −1 ( ca. 96% SRU) can be achieved with 95% initial Coulombic efficiency, appealing cyclability over 500 times, and a high-energy density of 392 Wh kg S+Zn −1 . These findings signify a paradigm shift and introduce transformative opportunities for the design of safe and high-energy aqueous batteries.
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RSC Advances
·
2025
·Vol. 15
·期 29
·23819-23826
·
IF 4.6
Yanhong Ding,Zhichao Gao,Rongpeng Lin,Yong Cao,Haoyang Liu,Yulin Zhou,Haifeng Xu,Jiayi Liu,Fangqi Ren,Yirong Zhu
Lithium–oxygen (Li–O2) batteries are considered an important candidate for the next generation of energy storage systems due to their ultra-high theoretical energy density (11 586 mA h g−1), but their slow kinetic reactions, high overpotential and cyclic instability seriously limit their practical applications. In this study, sulfur modified Mo2C (S@Mo2C) cathode materials were prepared by hydrothermal synthesis by sulfur (S) doping to optimize the electronic structure and catalytic activity of Mo2C (Mo2C). Experiments show that S@Mo2C exhibits significantly improved electrochemical performance compared to commercial Mo2C: its specific capacity is up to 3955 mA h g−1 (commercial material only 508 mA h g−1), the charge and discharge overpotential is reduced to 0.26 V (53.6%), and the capacity retention rate remains 77.8% after 250 cycles. X-ray diffraction (XRD), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS) analysis showed that the introduction of sulfur induced the formation of a heterostructure of MoS2/MoS3 in the Mo2C lattice, which enhanced the conductivity and oxygen reduction/precipitation (ORR/OER) activity of the material. In addition, sulfur doping promotes the formation of highly conductive amorphous Li2O2 and effectively inhibits the accumulation of insulating ring Li2O2, thus significantly improving the cycle stability and energy efficiency of the battery. This study provides a new structural regulation strategy for the design of high efficiency lithium oxygen battery catalysts.
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Chemical Science
·
2025
·
IF 7.4
Shengyuan Zhang,Hong Wen,Qianru Wang,Yongli Cai,Zhao LI,Liang Liu,Xuanwei Chang,Jianping Guo,Ping Chen
For many decades, selective hydrogenation N-heterocycles using H2 has been considered the exclusive domain of d-block metals. Herein, a series of solid-state s-block metal hydrides has been demonstrated to function as transition-metal-free catalysts for hydrogenation of quinoline, affording high yields of 1,2,3,4-tetrahydroquinoline. Under relatively mild conditions, the catalytic performance of the representative barium hydride (BaH2) is good enough to compete with some recently developed Fe, Co, Ni catalysts. Moreover, this transformation is remarkably simple and does not need any sophisticated ligands, co-catalysts, and additives when compared with the existing methodology. Mechanistic studies reveal that quinoline substrate can activate BaH2 in situ, generating a kind of catalytically active organobarium hydride species that effects the hydrogenation process. With the aid of theoretical modeling, the synergistic scenario between Lewis acidic barium cation and nucleophilic hydride anion in regioselective hydrogenation of quinoline is delineated, which shows a distinct working principle from the conventional transition metal catalysis. These findings provide insights into the discovery of new catalysts and/or reactivity that is complementary to that of the transition metals.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2025
·
IF 4.1
Heng-xue Zhou,Yi Long,Qiu-fang Zhong,Jia-liang Wang,Bo Liang,Ying-bang Yao,Daniel Q. Tan,Sheng-guo Lu,Tao Tao
A novel V 2 O 3 @C/S composite cathode material is prepared via a combined strategy consisting of solution synthesis, hydrothermal treatment and high-temperature carbonization, and all solid-state lithium‑sulfur (Li S) batteries with high-performance are constructed. The obtained V 2 O 3 @C host material demonstrates excellent properties, including good structural stability, superior electrical conductivity, strong interfacial compatibility, notable sulfur adsorption and high catalytic capabilities. At a current rate of 0.1C, the V 2 O 3 @C/S cathode delivers an initial capacity of 1125 mAh g −1 with a capacity retention of 67.1 % after 150 cycles in solid-state cells at the room temperature. At 0.5C, the capacity retention still remains 45.5 % after 250 cycles. Cyclic voltammetry, electrochemical impedance spectroscopy, and Tafel analysis further demonstrate the enhanced reaction kinetics and reduced charge-transfer resistance. This work provides a promising strategy for designing the high-performance cathode materials of solid-state Li S batteries.
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