CATALYSIS LETTERS
·
2020
·Vol. 150
·期 8
·2415-2426
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IF 2.482
Tang Kexin,Yi Yunfei,Gao Zhen,Jia Honghua,Li Yan,Cao Fei,Zhou Hua,Jiang Min,Wei Ping
Abstract In present study, we have mined a transaminase (TA) from Rhizobium sp. from the pool of fully sequenced genomes by using an ω-TA sequence from Vibrio fluvialis JS17 as a template in a BLASTP search. The protein sequence of the TA from Rhizobium sp. exhibits 53% sequence identity to that from V. fluvialis . The TA with S -selectivity showed close evolutionary relationship with a pyruvate transaminase from Alcaligenes denitrificans Y2k-2 and an S -selective aminotransferase from Sphaerobacter thermophilus . The gene of the ω-TA was inserted into pET-28a and functionally expressed in E. coli BL21. Results showed that the recombinant ω-TA has a specific activity of 7.46 U/mg at pH 8.0, 30 ℃. The substrate specificity test found the ω-TA presented significant reactivity toward aromatic amino donors and amino acceptors containing aldehydes. More importantly, the ω-TA also exhibited a good affinity towards some cyclic substrates. The homology model of the ω-TA was built by Discovery Studio and docking was performed to describe the relative activity towards some substrates. Graphic Abstract
研究领域:
生物化学
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分子生物学
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微生物学
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生物技术
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DOI
高影响因子
Food Bioscience
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2020
·
IF 3.067
Guozhong Zhao,Man Cui,Mengying Wang,Wen Chen,Jingjing Li,Yunping Yao
Lactobacillus strains are used for their probiotic effects and fermentation properties. The colonization of 16 Lactobacillus strains was determined based on animal experimentation and Real-Time Quantitative PCR. Strains expressed varied amounts of colonization in the mouse gut environment, the best was shown by L. plantarum CCFM737 and L. casei CCFM736, followed by other L. plantarum and L. casei strains. L. plantarum CCFM737 and L. casei CCFM736 both colonized at a significant level for at least 2 wk. L. plantarum and L. casei showed the best biological properties, including gastrointestinal tolerance, antimicrobial activity , use of oligosaccharides , tolerance of gastrointestinal juices and adhesive properties, especially the antimicrobial properties. A significant correlation between colonization of L. plantarum and L. casei and biological properties was observed.
研究领域:
微生物学
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肠胃病学
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分子生物学
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DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2018
·
IF 4.005
Shizong Wang,Yuming Hu,Jianlong Wang
A novel sulfamethoxazole (SMX)-degrading strain, Acinetobacter sp., was used to degrade other pharmaceutical compounds, including sulfadiazine (SD), sulfamethazine (SMT), trimethoprim (THM), triclosan (TCS), diclofenac (DFC) and carbamazepine (CBZ). The experimental results showed that Acinetobacter sp. can completely degrade SMX, SD and SMT, but with different mineralization efficiency. Acinetobacter sp. can mineralize 98.8% of SMX, while only 17.5% and 20.5% for SD and SMT, respectively. The intermediate products of SMX, SD and SMT degradation were tentatively identified. Based on the intermediates, it is inferred that the initial step for degrading sulfonamides by Acinetobacter sp. was the amidation of the amino groups in the benzene ring . The presence of methyl in the heterocyclic ring could induce the formation of methylase. By comparing the intermediates of SMX, SD and SMT degradation, it is concluded that Acinetobacter sp. preferred attacking the oxazole ring. However, Acinetobacter sp. cannot degrade THM, TCS, DFC and CBZ, while Acinetobacter sp. can still degrade SMX in the respective presence of THM, DFC and CBZ, although the degradation rate decreased. Moreover, the presence of TCS could completely inhibit the degradation of SMX by Acinetobacter sp.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
JOURNAL OF ANTIBIOTICS
·
2016
·Vol. 70
·期 2
·216-218
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IF 2.173
Gao Mei-yue,Qi Huan,Li Jian-song,Zhang Hui,Zhang Ji,Wang Ji-dong,Xiang Wen-sheng
Accessions GenBank/EMBL/DDBJ AF389344 KX118440
研究领域:
微生物学
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天然产物化学
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DOI
PubMed
高影响因子
MOLECULAR MICROBIOLOGY
·
2021
·Vol. 116
·期 3
·783-793
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IF 3.501
Yidong Zhou,Siyuan Gao,Mingliang Zhang,Wankui Jiang,Zhijian Ke,Jiguo Qiu,Jianhong Xu,Qing Hong
Salicylate is a typical aromatic compound widely distributed in nature. Microbial degradation of salicylate has been well studied and salicylate hydroxylases play essential roles in linking the peripheral and ring-cleavage catabolic pathways. The direct hydroxylation of salicylate catalyzed by salicylate-1-hydroxylase or salicylate-5-hydroxylase has been well studied. However, the CoA mediated salicylate 5-hydroxylation pathway has not been characterized in detail. Here, we elucidate the molecular mechanism of the reaction in the conversion of salicylate to gentisate in the carbaryl-degrading strain Rhizobium sp. X9. Three enzymes (salicylyl-CoA ligase CehG, salicylyl-CoA hydroxylase CehH and gentisyl-CoA thioesterase CehI) catalyzed the conversion of salicylate to gentisate via a route, including CoA thioester formation, hydroxylation and thioester hydrolysis. Further analysis indicated that genes cehGHI are also distributed in other bacteria from terrestrial environment and marine sediments. These genomic evidences highlight the role of this salicylate degradation pathway in the carbon cycle of soil organic compounds and marine sediments. Our findings of this three-step strategy enhanced the current understanding of CoA mediated degradation of salicylate.
研究领域:
微生物学
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环境科学
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生物化学
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DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 20
·7099
·
IF 4.927
Kuo Yong,Sidra Kaleem,Mingzhu Ma,Xiaoyuan Lian,Zhizhen Zhang
Marine-derivedPenicilliumfungi are one of the most important sources for the discovery of new bioactive natural products. This study characterized the isolation, structures, and antiglioma activities of twelve compounds, including three novel ones—penipyridinone B (1), 11S-(−)-penilloid A (2), and 11R,14E-(+)-penilloid A (3)—from the marine fungusPenicilliumsp. ZZ1750. The structures of the novel compounds were determined via extensive nuclear magnetic resonance (NMR) spectroscopic analyses, high-resolution electrospray ionization mass spectroscopy (HRESIMS) data, Mosher’s method, optical rotation (OR) calculations, and electronic circular dichroism (ECD) calculations. Penipyridinone B represents the first example of its structural type and showed potent antiglioma activity, with IC50values of 2.45 μM for U87MG cells and 11.40 μM for U251 cells. The known compounds of questiomycin A (9) and xanthocillin X (10) also showed antiproliferative activity against both U87MG and U251 cells, with IC50values of 13.65 μM to 22.56 μM. The antiglioma activity of questiomycin A and xanthocillin X may be related to the promotion of reactive oxygen species (ROS) production, the reduction of mitochondrial membrane potential (MMP), and the enhancement of caspase-3 enzyme activity.Keywords:marinePenicilliumsp. ZZ1750;penipyridinone B;11S-(−)-penilloid A;11R,14E-(+)-penilloid A;structure elucidation;antiglioma activity
研究领域:
天然产物化学
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药理学
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海洋生物学
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癌症研究
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DOI
PubMed
高影响因子
JOURNAL OF NATURAL PRODUCTS
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2019
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IF 4.257
Di Zhang,Wenwen Yi,Hengju Ge,Zhizhen Zhang,Bin Wu
The new streptoglutarimides A–J (1–10) and the known streptovitacin A (11) were isolated from a marine-derived actinomycete, Streptomyces sp. ZZ741. Structures of the isolated compounds were elucidated based on their HRESIMS data, extensive NMR spectroscopic analyses, ECD calculations, Mosher′s method, and a single-crystal X-ray diffraction experiment. Streptoglutarimide H (8) and streptovitacin A (11) showed potent antiproliferative activity against human glioma U87MG and U251 cells with IC50 values of 1.5–3.8 μM for 8 and 0.05–0.22 μM for 11. All isolated compounds exhibited antimicrobial activity with MIC values of 9–11 μg/mL against methicillin-resistant Staphylococcus aureus, 8–12 μg/mL against Escherichia coli, and 8–20 μg/mL against Candida albicans.
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Haikun Zhang,Yanyu Sun,Manman Cheng,Xiaori Sui,Yanyan Huang,Xiaoke Hu
As a toxic element of global concern, the elevated concentration of antimony (Sb) in the environment has attracted increasing attention. Microorganisms have been reported as important driving forces for Sb transformation. Iron (Fe) is the most important metal associated element of Sb, however, how Fe-bearing minerals affect the biological transformation of Sb is still unclear. In this study, the effects of Fe-bearing minerals on biological Sb(V) reduction were investigated by employing a marine Shewanella sp. CNZ-1 (CNZ-1). Our results showed that the presence of hematite, magnetite and ferrihydrite (1 g/L) resulted in a decrease in Sb(III) concentration of ~19–31 % compared to the Fe(III)-minerals free system. The calculated Sb(V) reduction rates are 0.0256 (R 2 0.71), 0.0389 (R 2 0.87), 0.0299 (R 2 0.96) and 0.0428 (R 2 0.95) h −1 in the hematite-, magnetite-, ferrihydrite-supplemented and Fe(III)-minerals free systems, respectively. The cube-shaped Sb 2 O 3 was characterized as a reductive product by using XRD, XPS, FTIR, TG and SEM approaches. Differential proteomic analysis showed that flagellar protein, cytochrome c , electron transfer flavoprotein, nitrate reductase and polysulfide reductase (up-regulation >1.5-fold, p value <0.05) were supposed to be included in the electron transport pathway of Sb(V) reduction by strain CNZ-1, and the key role of nitrate reductases was further highlighted during this reaction process based on the RT–qPCR and confirmatory experiments. Overall, these findings are beneficial to understand the environmental fate of Sb in the presence of Fe-bearing minerals and provide guidance in developing the bacteria/enzyme-mediated control strategy for Sb pollution.
研究领域:
环境微生物学
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污染生态学
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DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2026
·
IF 5
Zhilai Lu,Zhe Luo,Min Liao,Xiaomei Xie,Mingdi Fan,Yue Xiao,Yunqiang Zhu
Two plant growth promoting bacteria (PGPB) were isolated. • PGPB could significantly enhance the biomass and protein content of Chlorella. • PGPB could enhancing the nitrogen assimilation and cell division of Chlorella. • PGPB could inhibit the flow of carbon metabolism to fat. The co-cultivation of microalgae with bacteria that stimulate microalgae growth and metabolic changes is a promising strategy for improving microalgae yield and quality. In this study, two plant growth promoting bacteria (PGPB), Bacillus safensis BAI-6 (BAI-6) and Bacillus altitudinis FEN-9 (FEN-9), were isolated from a long-term open cultivation system of Chlorella sp. LZ-29. Both strains were confirmed to produce the growth-promoting phytohormone indole-3-acetic acid (IAA) and vitamin B12. The changes in biomass, protein, fat, polysaccharide, pigment and other active components of Chlorella sp. LZ-29 under co-cultivation with these strains were investigated. Compared with the axenic Chlorella culture, co-cultivation with BAI-6 or FEN-9 alone increased biomass by 46.57% and 58.75%, respectively, while the dual-strain co-culture achieved the highest biomass increase of 66.46%. Protein content increased by 7% with BAI-6, 2.56% with FEN-9, and 13.37% in the dual-strain co-culture. Conversely, fat content decreased by 3.05%, 3.67%, and 3.48%, respectively, while polysaccharide content also declined in all co-culture treatments ( p < 0.05). The synergistic effect of BAI-6 and FEN-9 in dual co-culture resulted in significantly enhanced biomass and protein production ( p < 0.01), likely due to increased secretion of IAA and vitamin B12, which promoted nitrogen assimilation and cell division, while inhibiting carbon flux towards lipid synthesis. These findings demonstrate that the dual-strain co-culture system has significant potential for enhancing both the yield and protein content of Chlorella sp. LZ-29.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Qinyong Liu,Xiaowei Su,Jian Liu,Baozhu Wang,Huihui Li,Jun Sun,Hongfei Li,Xiaoyu Gu,Sheng Zhang
The widespread application of polyurea (PUA) in protective coatings typically requires excellent flame retardancy due to real-world usage scenarios. However, the addition of flame retardant additives often compromises the material’s mechanical properties. In this study, a multifunctional reactive additive, MX-SP-NH 2 , was creatively prepared by covalently grafting spirocyclic pentaerythritol bisphosphorate disphosphoryl chloride (SPDPC) onto MXene (Ti 3 C 2 T x ) surfaces, followed by the introduction of 1,3-propanediamine (PDA) by the reaction with SPDPC. MX-SP-NH 2 was then incorporated into PUA, participating in its crosslinking process and significantly enhancing both its mechanical strength and flame retardancy. Compared to the control PUA sample, the micro-crosslinked PUA sample with just 1.0 wt% MX-SP-NH 2 demonstrated a 184.3% increase in tensile strength, a 126.1% increase in elongation at break, and a 436.3% increase in toughness. The flammability characterization revealed that the peak heat release rate, peak smoke production rate, and peak CO production rate were reduced by 31.7%, 28.9%, and 61.5%, respectively, indicating a substantial improvement in fire safety. Additionally, the flame retardant PUA-based flexible strain sensors demonstrated stable electrical signal responsiveness. This work has opened up new potential applications of PUA in flexible electronics.
研究领域:
材料科学
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聚合物化学
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防火安全工程
·
柔性电子
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Yanyu Zhou,Fucun Liu,Meile Yuan,Xianhua Liu,Qipei Li,Hongwei Zhao
Ocean warming threatens the health of corals globally, and superimposed coastal environmental pollution can result in severe and irreversible coral bleaching. However, the responses of the coral symbiont Symbiodiniaceae to multiple stresses remain largely unknown. This study investigated the response of the coral symbiotic algae Cladocopium sp. to short-term exposure (4 days) to an environmentally relevant concentration (1 μg L −1 ) of the photosystem II (PSII) herbicide prometryn under heat stress (32 ℃) through physiological and omic analyses. These results showed that co-stress affected the photosynthetic efficiency of Cladocopium sp. negatively. Overproduction of reactive oxygen species and subsequent oxidative stress under co-stress activated distinct regulatory pathways in Cladocopium sp. Transcriptomic and proteomic analyses revealed that prometryn exacerbated heat stress-induced photosystem damage and reduced the regulatory capacity of Cladocopium sp. Moreover, co-stress disrupted energy metabolism, and further impaired nitrogen assimilation and nutrient transfer processes, potentially compromising the symbiotic potential between corals and Symbiodiniaceae. In summary, this study offers a valuable insight into understanding the molecular responses of Symbiodiniaceae to thermal and prometryn co-stress. It helps uncover the potential toxicity mechanisms induced by herbicide on coral symbionts in the context of climate change.
研究领域:
海洋生物学
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环境科学
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分子生态学
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DOI
PubMed
高影响因子
PLoS One
·
2015
·Vol. 10
·期 9
·e0137066
·
IF 3.234
Yan Zhang,Junling Shi,Laping Liu,Zhenhong Gao,Jinxin Che,Dongyan Shao,Yanlin Liu
Pinoresinol diglucoside (PDG) and pinoresinol (Pin) are normally produced by plant cells via the phenylpropanoid pathway. This study reveals the existence of a related pathway in Phomopsis sp. XP-8, a PDG-producing fungal strain isolated from the bark of the Tu-chung tree ( Eucommiaulmoides Oliv.). After addition of 0.15 g/L glucose to Phomopsis sp. XP-8, PDG and Pin formed when phenylalanine, tyrosine, leucine, cinnamic acid, and p -coumaric acid were used as the substrates respectively. No PDG formed in the absence of glucose, but Pin was detected after addition of all these substrates except leucine. In all systems in the presence of glucose, production of PDG and/or Pin and the accumulation of phenylalanine, cinnamic acid, or p -coumaric acid correlated directly with added substrate in a time- and substrate concentration- dependent manner. After analysis of products produced after addition of each substrate, the mass flow sequence for PDG and Pin biosynthesis was defined as: glucose to phenylalanine, phenylalanine to cinnamic acid, then to p -coumaric acid, and finally to Pin or PDG. During the bioconversion, the activities of four key enzymes in the phenylpropanoid pathway were also determined and correlated with accumulation of their corresponding products. PDG production by Phomopsis sp. exhibits greater efficiency and cost effectiveness than the currently-used plant-based system and will pave the way for large scale production of PDG and/or Pin for medical applications.
研究领域:
微生物学
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生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）