INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.5
Dongshan Chen,Yuan Zeng,Yuanchen Liu,Haochen Cui,Yixuan Zhao,Lei Yan,Yuanwei Zang
Clear cell renal cell carcinoma (ccRCC) progression is closely associated with pro-tumorigenic neutrophil extracellular traps (NETs) within the tumor microenvironment; however, the upstream molecular mechanisms remain poorly defined. Through integrated multi-omics analysis of ccRCC cohorts, we identified ITPKB as a key downregulated gene associated with NET formation. Subsequent functional validation, including dual-luciferase reporter assays, confirmed that ITPKB is a direct target of the miR-301b-3p. Mechanistically, the loss of ITPKB prevents the ubiquitination-mediated degradation of PARP1, leading to its stabilization and accumulation. Stabilized PARP1 acts as a transcriptional co-activator for NF-κB, triggering a robust secretion of IL-8, which recruits neutrophils and induces their transformation into NETs. These NETs facilitate tumor cell migration and adhesion while conferring resistance to tyrosine kinase inhibitors (sunitinib and sorafenib) and anti-PD-1 therapy. In vivo, the treatment with DNase1 to disrupt NETs effectively reversed this therapeutic resistance. Our findings unveil the miR-301b-3p/ITPKB/PARP1/IL8/NETs axis as a novel mechanistic link between tumor-intrinsic signaling and neutrophil-driven immunosuppression, providing a solid experimental foundation for developing combination therapeutic strategies for advanced ccRCC.
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DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2024
·Vol. 12
·期 17
·10260-10268
·
IF 10.7
Yingfen Li,Xingye Chen,Runxi Wang,Nian Zhou,Fang Huang,Jun Zhao,Zhenghua Su,Shuo Chen,Guangxing Liang
In this study, Sb2S3 was introduced into the two-step selenization process to promote bottom grain growth and reduce the surface roughness of the Cu2ZnSn(S,Se)4 light-absorber layer. The introduction of Sb doping in Cu2ZnSn(S,Se)4 can passivate bulk and interface defects and a small conduction band offset (CBO) can be found, which facilitate the improvement of heterojunction carrier transport properties. Similarly, simulations illustrated that Sb doping improved device efficiency by controlling bulk and interface defects and band alignment. This optimized method facilitated the penetration of Sb, resulting in a high-quality CZTSSe absorber layer. Ultimately, the research culminated in a significant enhancement, yielding a solar cell with an impressive efficiency of 13.11%. These findings offer new possibilities for kesterite solar cells and advance the potential of CZTSSe materials in thin film photovoltaic applications.
研究领域:
材料科学
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光伏技术
·
半导体物理
·
薄膜太阳能电池
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无机功能材料
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固态电子学
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DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2018
·
IF 2.732
Ruoshan Lei,Xinyao Luo,Ziyuan Yuan,Huanping Wang,Feifei Huang,Degang Deng,Shiqing Xu
In this work, a new optical thermometer relying on highly temperature-dependent 1 D 2 Pr 3+ emission and temperature insensitive Y 2 Ti 2 O 7 trap emission is reported. Based on the fluorescence intensity ratio (FIR) between them, Pr 3+ : Y 2 Ti 2 O 7 with remarkably high absolute and relative sensitivities of ~ 983 × 10 −4 K −1 and 5.25% K −1 as well as good signal discriminability is realized. It also exhibits a tunable luminescent color from red to purplish blue with temperature from 289 to 573 K. The strong thermal quenching of 1 D 2 Pr 3+ emission is mainly related to the charge transfer state (CTS). Similarly, Pr 3+ : Gd 2 Ti 2 O 7 phosphor also demonstrates a favorable temperature sensing feature, indicating the temperature sensing strategy based on the combination of host trap emission and CTS interfered Pr 3+ luminescence is a promising pathway to develop high-performance optical thermometric materials.
研究领域:
光学传感
·
发光材料
·
测温技术
·
稀土掺杂
·
固体物理
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DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2021
·
IF 5.316
Xin Li,Xusheng Wang,Rui Hu,Yanxia Li,Xi Yao
We report a novel mechanoluminescent phosphor Li 1−x Ca x Si x Ta 1−x O 3 :0.01Pr 3+ (x = 0–0.04). The crystal structure, photoluminescence (PL), optical diffuse reflectance spectra, afterglow (AG), fluorescence decay curves, and mechanoluminescence (ML) properties were investigated. To clarify the defect types and the mechanoluminescence mechanisms, an X-ray photoelectron spectroscopy (XPS) and thermoluminescence curve (TL) were measured. The dopants Ca 2+ and Si 4+ ions occupied the Li+ and Ta 5+ sites, respectively. An optimal sample Li 0.98 Ca 0.02 Si 0.02 Ta 0.98 O 3 :Pr 3+ was obtained with great enhancement in mechanoluminescence intensity, higher than that of non-doped sample LiTaO 3 :Pr 3+ by 262%. And the compression threshold, over which the ML intensity could not maintain the linear increase, has also been greatly improved. Furthermore, the stable output of Tribo-L was achieved, which was visible to the naked eye. A probable mechanism was proposed to illustrate the mechanoluminescent process in Li1-xCaxSixTa1-xO3:0.01Pr3 + (x = 0–0.04). The study indicates that adjusting trap distribution by substituting host ions may be a novel way to develop high-quality mechanoluminescence.
研究领域:
材料科学
·
光学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Chunjian Wu,Li Wang,He Li,Shujuan Yu
The presence of potential carcinogen 4(5)-Methylimidazole (4-MeI) in foods causes much concern, stressing the need of a sensitive determination means. Here, we proposed a high-sensitivity method to determine 4-MeI in biscuits using dansyl chloride derivatization and disperse liquid-liquid micro-extraction (DLLME) followed by a liquid chromatography-ion trap mass spectrometry (LC-IT-MS) analysis. This developed method was subsequently compared to the solid phase extraction combining with liquid chromatography-triple quadrupole mass spectrometry (SPE-LC-QqQ-MS) method. The optimized derivatization conditions were 30 °C and 10 min. Results suggested that the column retention time (RT) was significantly extended, and the MS signal response of 4-MeI-dansyl derivative was also amplified. The developed DLLME-LC-IT-MS method in 4-MeI determination provided satisfactory linearity in a range of 0.5–300 ng/mL (R 2 > 0.9991), inter-day accuracy (87-102%) and precision (%RSD ≤ 13.6%) with the quantification limit of 0.2 ng/mL, which obtained similar results comparing to the conventional SPE-LC-QqQ-MS method.
研究领域:
分析化学
·
食品科学
·
质谱分析
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产品关联
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2018
·
IF 4.556
Jiamin Wu,Zhi Li,Zeping Yang,Ling Guo,Ye Zhang,Huihui Deng,Cuifeng Wang,Min Feng
It is not efficient enough using the current approaches for tumor-selective drug delivery based on the EPR effect and ligand–receptor interactions, and they have largely failed to translate into the clinic. Therefore, it is urgent to explore an enhanced strategy for effective delivery of anticancer agents. Clinically, many cancers require large amounts of glutamine for their continued growth and survival, resulting in circulating glutamine extraction by the tumor being much greater than that for any organs, behaving as a “glutamine trap”. In the present study, we sought to elucidate whether the glutamine-trap effect could be exploited to deliver therapeutic agents to selectively kill cancer cells. Here, a macromolecular glutamine analogue, glutamine-functionalized branched polyethylenimine (GPI), was constructed as the carrier to deliver anti-CD47 siRNA for the blockage of CD47 “don’t eat me” signals on cancer cells. The GPI/siRNA glutamine-rich polyplexes exhibited remarkably high levels of cellular uptake by glutamine-dependent lung cancer cells, wild-type A549 cells (A549WT), and its cisplatin-resistant cells (A549DDP), specifically under glutamine-depleted conditions. It was noted that the glutamine transporter ASCT2 was highly expressed both on A549WT and A549DDP but with almost no expression in normal human lung fibroblasts cells. Inhibition of ASCT2 significantly prevented the internalization of GPI polyplexes. These findings raised the intriguing possibility that the glutamine-rich GPI polyplexes utilize the ASCT2 pathway to selectively facilitate their cellular uptake by cancer cells. GPI further delivered anti-CD47 siRNA efficiently both in vitro and in vivo to downregulate the intratumoral mRNA and protein expression levels of CD47. CD47 functions as a “don’t eat me” signal and binds to the immunoreceptor SIRPα inducing evasion of phagocytic clearance. GPI/anti-CD47 siRNA polyplexes achieved significant antitumor activities both on A549WT and A549DDP tumor-bearing nude mice. Notably, it had no adverse effect on CD47-expressing red blood cells and platelets, likely because of selective delivery. Therefore, the glutamine-rich carrier GPI driven by the glutamine-trap effect provides a promising new strategy for designing anticancer drug delivery systems.
研究领域:
肿瘤学
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药学
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分子生物学
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癌症治疗
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2022
·Vol. 10
·期 48
·18404-18414
·
IF 8.067
Shaoxing Lyu,Pengshang Zhou,Jiaren Du,Xiaomeng Wang,Tengyue Wang,Panqin Wang,Henggang Wang,Shan Sun,Hengwei Lin
Near-infrared (NIR) emitting phosphors with persistent luminescence have attracted much interest in the past decade, due to their widespread applications in medical diagnostics, night-vision surveillance, multi-level anti-counterfeiting, and security encryption. Although NIR luminescent materials have been extensively investigated, it is challenging to explore novel phosphors or new compositions with tunable NIR persistent luminescence performance. Herein, chemical unit co-substitution is explored in a series of NIR emitting Zn1−2xLixGa2+xO4:Cr3+ solid solutions. Substitution with [Li+–Ga3+] for the [Zn2+–Zn2+] unit results in tunable emission in the deep-red and NIR regions coming from the typical Cr3+ emission of the 2E → 4A2 and 4T2 → 4A2 transition. Upon UV or X-ray irradiation, intense NIR persistent luminescence is realized and composition-related variation of trap distributions and densities are identified. Importantly, a higher trap density of the Cr3+-doped solid solutions (i.e., x = 0.2–0.4) than that of the benchmark NIR persistent phosphor of ZnGa2O4:Cr3+ and LiGa5O8:Cr3+ is achieved. This work proves the feasibility of chemical unit co-substitution in developing new NIR persistent phosphors, which can motivate further exploration of luminescent materials with novel properties.
研究领域:
材料科学
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光学
·
光子学
·
纳米技术
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产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2021
·Vol. 9
·期 30
·9619-9626
·
IF 7.393
Shenghui Zheng,Junpeng Shi,Xiaodan Wang,Xiaoyan Fu,Hongyong Fan,Hongwu Zhang
Here, we have developed a novel thermosensitive technique based on shallow-trap persistent luminescence to achieve high-resolution and long-duration texture imaging of fingerprints even through latex gloves. A thermosensitive flexible film that consists of a transparent silica gel embedded with a shallow-trap Bi2Ga4O9:Eu3+,Cr3+ (BGO) phosphor microarray exhibits broadband near-infrared PersL, which enables sensitive temperature sensing using a thermally stimulated luminescence (TSL) method. The uniform surface conical arrays of the BGO film ensure a high thermal sensing resolution. Only a simple touch is enough to induce thermal distribution that can be easily visualized as luminescence using an EMCCD camera. Furthermore, the BGO film responds rapidly to small temperature fluctuations with high sensitivity (8.10 ± 0.476% K−1), enabling fingerprint imaging even through latex gloves in only a few seconds. This imaging strategy is very promising for sensitive temperature sensing without power supply, and it also opens new application opportunities for shallow-trap PersL materials.
研究领域:
材料科学
·
光学工程
·
生物识别技术
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Miao Chang,Jiahao Ren,Yan Wei,Jie-Xin Wang,Qingyuan Yang,Dahuan Liu,Jian-Feng Chen
It is an extremely challenging task to capture C 3 H 8 from the C 3 H 8 /C 3 H 6 mixture, which requires an adsorbent with a specific pore structure. Herein, an ultramicroporous pillared metal–organic framework Ni(ADC)(TED) 0.5 is reported for efficient C 3 H 8 /C 3 H 6 separation. Theoretical calculations revealed that the ADC ligand may form a splint-like pore confinement structure induced by the introduction of guest molecules to construct a propane-trap in the framework. As a result, excellent separation performance for C 3 H 8 /C 3 H 6 is obtained. A record-high IAST-predicted selectivity (6.4), C 3 H 8 /C 3 H 6 uptake ratio (111.0%), C 3 H 8 Henry constant (2159.2 mmol g −1 bar −1 ), and separation potential (1943.0 mmol L -1 ) exceed those in porous adsorbents reported so far. Breakthrough experiments indicate that Ni(ADC)(TED) 0.5 can completely separate C 3 H 8 /C 3 H 6 mixture with the highest C 3 H 6 productivity (0.085 mmol g −1 ) and good regenerability. These results show that this material has great potential application in C 3 H 8 /C 3 H 6 separation.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
CELLULAR SIGNALLING
·
2021
·
IF 4.315
Jinpeng Zhuang,Hua Ning,Maoqing Wang,Wei Zhao,Yongbin Jing,Xiaoqi Liu,Jianing Zu,Pengyu Kong,Xiaoyan Wang,Changhao Sun,Jinglong Yan
During osteoporosis, fat mass and obesity-associated protein (FTO) promotes the shift of bone marrow mesenchymal stem cells to adipocytes and represses osteoblast activity. However, the role and mechanisms of FTO on osteoclast formation and bone resorption remain unknown. In this study, we investigated the effect of FTO on RAW264.7 cells and bone marrow monocytes (BMMs)-derived osteoclasts in vitro and observed the influence of FTO on ovariectomized (OVX) mice model to mimic postmenopausal osteoporosis in vivo. Results found that FTO was up-regulated in BMMs from OVX mice. Double immunofluorescence assay showed co-localization of FTO with tartrate-resistant acid phosphatase (TRAP) in femurs of OVX mice. FTO overexpression enhanced TRAP-positive osteoclasts and F-actin ring formation in RAW264.7 cells upon RANKL stimulation. The expression of osteoclast differentiation-related genes, including nuclear factor of activated T cells c1 (NFATc1) and c-FOS, was upregulated in BMMs and RAW264.7 cells after FTO overexpression. FTO overexpression induced the phosphorylation and nuclear translocation of factor-kappa B (NF-κB) p65 in BMMs and RAW264.7 cells exposed to RANKL. ChIP and dual-luciferase assays revealed that FTO overexpression contributed to RANKL-induced binding of NF-κB to NFATc1 promoter. Rescue experiments suggested that FTO overexpression-mediated osteoclast differentiation was suppressed after intervention with a NF-κB inhibitor pyrrolidine dithiocarbamate . Further in vivo evidence revealed that FTO knockdown increased bone trabecula and bone mineral density, inhibited bone resorption and osteoclastogenesis in osteoporotic mice. Collectively, our research demonstrates that downregulated FTO inhibits bone resorption and osteoclastogenesis through NF-κB inactivation, which provides a novel reference for osteoporosis treatment.
研究领域:
骨骼生物学
·
分子生物学
·
细胞生物学
·
内分泌学
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产品关联
DOI
PubMed
高影响因子
COMPOSITES SCIENCE AND TECHNOLOGY
·
2020
·
IF 7.094
Kai Yin,Qing Xie,Haoou Ruan,Qijun Duan,Fangcheng Lü,Xingming Bian,Tian Zhang
Composite dielectrics with high vacuum insulation properties are integral to today's advanced energy transmission. In this study, 5% mol Yttrium doped BiFeO 3 sub-particles were synthesized by a facile sol-gel route and then were blended with epoxy resin as filler to prepare the epoxy composites (BY5FO/EP) with a wide range of filler content . The multi-electrical investigations were employed, and the results show the BY5FO/EP composites present ultra-high surface insulation performance. As the concentration of filler increases, the dielectric constant of the BY5FO/EP composites exhibited sine-shaped variations. Considering the multiple-layers of filler particles , we determined the dielectric model of composites for a wide range of filler contents by simulation. Meanwhile, the trap depths of BY5FO/EP composites were calculated according to Isothermal Surface Potential Decay, and we found the trapping stability is of sinusoidal relevance with the filler content. The simultaneous sine-variations of dielectric polarization and trap depth lead to the vacuum surface breakdown strength of the composite increased by 111% with the optimal filler content. The results in this paper provide a prospective strategy to realize ultra-high surface insulation in a vacuum and evidence to the applicability of the Electron Triggered Polarization Relaxation theory.
研究领域:
材料科学
·
电气工程
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DOI
高影响因子
Acta Biomaterialia
·
2022
·
IF 10.633
Feng Li,Liming Lin,Jiaying Chi,Hui Wang,Minqun Du,Disang Feng,Liqing Wang,Rui Luo,Hangping Chen,Guilan Quan,Jianfeng Cai,Xin Pan,Chuanbin Wu,Chao Lu
Focal bacterial infections are often difficult to treat due to the rapid emergence of antibiotic-resistant bacteria, high risk of relapse, and severe inflammation at local lesions. To address multidrug-resistant skin and soft tissue infections, a bacteria-absorbing sponge was prepared to involve a “trap-and-kill” mechanism. The system describes a guanidinium-rich lipopeptide functionalized lyotropic liquid-crystalline hydrogel with bicontinuous cubic networks. Amphiphilic lipopeptides can be spontaneously anchored to the lipid-water interface, exposing their bacterial targeting sequences to enhance antibacterial trapping/killing activity. Computational simulations supported our structural predictions, and the sponge was confirmed to successfully remove ∼98.8% of the bacteria in the medium. Release and degradation behavior studies indicated that the bacteria-absorbing sponge could degrade, mediate enzyme-responsive lipopeptide release, or generate ∼200 nm lipopeptide nanoparticles with environmental erosion. This implies that the sponge can effectively capture and isolate high concentrations of bacteria at the infected site and then sustainably release antimicrobial lipopeptides into deep tissues for the eradication of residual bacteria. In the animal experiment, we found that the antibacterial performance of the bacterial-absorbing sponge was significant, which demonstrated not only a long-term inhibition effect to disinfect and avoid bacterial rebound, but also a unique advantage to protect tissue from bacterial attack. Statement of significance Host defense peptides/peptidomimetics (HDPs) have shown potential for the elimination of focal bacterial infections, but the application of their topical formulations suffers from time-consuming preparation processes, indistinctive toxicity reduction effects, and inefficient bacterial capture ability. To explore new avenues for the development of easily prepared, low-toxicity and high-efficiency topical antimicrobials, a guanidinium-rich lipopeptide was encapsulated in a lyotropic liquid-crystalline hydrogel (denoted as “bacteria-absorbing sponge”) to achieve complementary superiorities. The superior characteristic of the bacteria-absorbing sponge involves a “trap-and-kill” mechanism, which undergoes not only a long-term inhibition effect to disinfect and avoid bacterial rebound, but also effective bacterial capture and isolating action to confine bacterial diffusion and protect tissues from bacterial attack.
研究领域:
药学
·
微生物学
·
材料科学
·
生物医学工程
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DOI
PubMed
高影响因子
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