JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2020
·
IF 3.721
Caiyun Zhang,Jiaxiong Zeng,Wenguang Xiong,Zhenling Zeng
A rapid, simple and reliable method using amoxicillin-D4 as internal standard (IS) for determination of amoxicillin (AMO) in porcine tissues was developed. The analytical methodology used a phosphate buffer (0.01 M, pH = 6.3) with trichloroacetic acid (50 mg/mL) for extraction and Oasis® HLB cartridges for clean-up followed by UPLC-MS/MS analysis. The optimized method was validated according to EU guideline 2002/657/EC and FDA guideline. The LOQ in muscle, fat, liver and kidney were set to 10 μg/kg which was below the maximum residue limits, while the LOD were set to 5 μg/kg. Results showed that using AMO-D4 as IS can effectively eliminate relative matrix interference and improve the recovery and precision. A good linearity was observed at the range of 10−500 μg/kg with a correlation coefficients (R 2 ) larger than 0.99. The mean recoveries were higher than 87.0 %, and the RSDs were lower than 11.2 %. This method could be applied to conveniently analysis the residue of AMO in different porcine tissues.
研究领域:
兽医学
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分析化学
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食品安全
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DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 6
·2494
·
IF 4.6
Yanmei He,Fangfang Wu,Zhien Tan,Mengli Zhang,Taiping Li,Aihua Zhang,Jianhua Miao,Min Ou,Lihuo Long,Hui Sun,Xijun Wang
Jigucao capsules (JGCC) have the effects of soothing the liver and gallbladder and clearing heat and detoxification. It is a good medicine for treating acute and chronic hepatitis cholecystitis with damp heat of the liver and gallbladder. However, the existing quality standard of JGCC does not have content determination items, which is not conducive to quality control. In this study, serum pharmacochemistry technology and UNIFI data processing software were used to identify the blood prototype components and metabolites under the condition of the obvious drug effects of JGCC, and the referenced literature reports and the results from in vitro analysis of JGCC in the early stage revealed a total of 43 prototype blood components and 33 metabolites in JGCC. Quality markers (Q-markers) were discovered, such as abrine, trigonelline, hypaphorine and isoschaftoside. In addition, ultra-high-performance liquid chromatography–triple quadrupole mass spectrometry (UPLC-QQQ-MS) was used to determine the active ingredients in JGCC. The components of quantitative analysis have good correlation in the linear range with R2≥ 0.9993. The recovery rate is 93.15%~108.92% and the relative standard deviation (RSD) is less than 9.48%. The established UPLC-MS/MS quantitative analysis method has high sensitivity and accuracy, and can be used for the quality evaluation of JGCC.Keywords:serum pharmacochemistry;jigucao capsule;quality markers;ultra-high-performance liquid chromatography-mass spectrometry;quality evaluation
研究领域:
药理学
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分析化学
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中药学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2018
·
IF 3.849
Zhengang Zhao,Huaifeng Yan,Rui Zheng,Muhammad Saeed Khan,Xiong Fu,Zi Tao,Zhanying Zhang
Anthocyanins in rind extracts of three sugarcane cultivars, ROC 22, Haitang 22 and Guitang 21, were characterized using ultra performance liquid chromatography (UPLC) combined with electrospray ionization quadrupole-time-of-flight tandem mass spectrometry (ESI-QTOF-MS/MS). A total of thirteen anthocyanins were identified and quantified. Except for cyanidin-3-glucoside, twelve anthocyanins were reported for the first time from sugarcane. The total anthocyanin content (TAC) varied significantly from 10.8 (Haitang 22) to 132.0 μg/g dry rind weight (DW) (ROC 22). Regarding single anthocyanins, ROC 22 contained 79.8 μg/g DW cyanidin-3-glucoside but this anthocyanin was not identified in the other two cultivars. Instead, Guitang 21 contained 94.6 μg/g DW malvidin-3- p -coumaroyl-rhamnoside-5-glucoside. The rinds of red sugarcane cultivars ROC 22 and Guitang 21 had higher total antioxidant activities than green sugarcane Haitang 22, which was attributed to much higher contents of free and total phenolics. This study provides useful information for the production of valuable nutraceuticals from sugarcane.
研究领域:
植物化学
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食品科学
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营养学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Haizhi Huang,Na Li,Ya Chen,Xuping Shentu,Xiaoping Yu,Zihong Ye
A novel extraction adsorbent composite of MWCNTs/NH2-MIL-101(Fe) was synthesized, and was used to extract 6 kinds of neonicotinoid pesticides in medicine and food homology products. The composite was characterized by scanning electron microscope (SEM), fourier transform-infrared (FT-IR) spectroscopy, and X-ray powder diffraction (XRD). MWCNTs were enveloped around MOFs to provide physical support for the crystal structure. The adsorbent has higher adsorption capacity and reusability than pure NH2-MIL-101(Fe). Combined with UPLC-MS/MS, the method showed the low limit of detection (LOD) and limit of quantitation (LOQ) of 0.01–0.07 μg/kg and 0.04–0.22 µg/kg, respectively. It exhibited high extraction recovery of 77.86–101.10% for neonicotinoid pesticides in spiked samples. Meanwhile, this novel method could be successfully employed for the detection of other medicine and food homology products. Compared with previous reports, this method has advantages in detection limit and extraction recovery, indicating that it can be a preferential choice for the detection of neonicotinoid pesticides.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2025
·
IF 2.8
Meiling Zhang,Siyu Zhuo,Fangmei Zhang,Jianshe Ma,Lufeng Hu,Xianqin Wang
The ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) technique was utilized to detect rubusoside in mouse plasma and evaluate its pharmacokinetic behavior following oral administration at doses of 10 mg/kg and 20 mg/kg, as well as intravenous administration at 10 mg/kg. Plasma samples were prepared using a protein precipitation method employing acetonitrile as the precipitating agent and isoscoparin as the internal standard (IS). Chromatographic separation was performed on a UPLC high strength silica (HSS) T3 column under gradient elution conditions, using acetonitrile and 0.1 % formic acid in water as the mobile phase system. The calibration curve for rubusoside demonstrated excellent linearity across a concentration range of 3.5 to 1120 ng/mL. Both intra-day and inter-day precision values remained below 7 %, with accuracy ranging from 92.3 % to 108.1 %. Matrix effects were consistently within the range of 107.1 % to 110.0 %, and the recovery rate exceeded 74.2 %. Following oral administration, the absolute bioavailability of rubusoside was determined to be 1.00 % and 0.98 % at doses of 10 mg/kg and 20 mg/kg, respectively. These findings confirm that the developed UPLC-MS/MS method effectively supports the pharmacokinetic evaluation of rubusoside
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2025
·
IF 12.5
Qing-Hua Liu,Liu Yang,Yue Wang,Hao-Ming Xiong,Cai-Yun Wang,Guo-Yuan Zhu,Li-ping Bai,Zhi-Hong Jiang,Wei Zhang
Polygonatum odoratum ( P. odoratum ) oligosaccharides (POO) are bioactive compounds with complex structures. Since the precise structure of POO remains undefined, their pharmacological development and clinical application have been hindered. This study developed a universal strategy to elucidate oligosaccharide structures of P. odoratum by combining Orbitrap high-resolution mass spectrometry (Orbitrap-HRMS) and enzymatic hydrolysis. Initially, monosaccharide constituents of free oligosaccharides (POO) and polysaccharides (POP) in P. odoratum were quantitatively analyzed. The accurate mass values, retention time, and sequence-specific fragment information facilitated initial characterization by comparing with the self-established oligosaccharide library based on these monosaccharides. Due to the limitations of MS in precisely identifying isomer structures, enzymatic hydrolysis was further employed to distinguish the glycosidic linkage and oligosaccharide type. Fructanase mixture, endo -1,4-β-mannanase, exo -1,3-β-D-glucanase, α-galactosidase and amyloglucosidase were used to identify hexose-based isomers, while α-L-arabinofuranosidase and endo -1,4-β-xylanase were used for pentose-based isomers. The study revealed that POO primarily consisted of fructooligosaccharides, and its isomers, such as 1-kestose, 6-kestose, and neokestose, were clarified. POP mainly comprised galactomannan, with the branching site at α-(1 → 6)-galactoside in POPO determined for the first time in P. odoratum . This strategy provides a straightforward methodology for analyzing plant oligosaccharides, establishing a structure analytical foundation for further pharmacological research and potential clinical applications.
研究领域:
植物学
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天然产物化学
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分析化学
·
糖生物学
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植物化学
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产品关联
DOI
PubMed
高影响因子
Letters in Drug Design & Discovery
·
2025
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IF 1.6
Huang Huang,Xiuhui Guo,Siqi Quan,Xiangli Yan,Yucheng Li,Erping Xu
Background Depression is a mental disorder characterized by persistent low mood and cognitive impairment. Chaihu Jia Longgu Muli Decoction (CLMD) is an effective and relatively safe therapeutic method for depression. However, the chemical composition of CLM and its underlying mechanisms remain unclear. Objective This study aimed to explore the potential mechanisms of CLMD in treating depression by integrating UPLC-MS/MS and network pharmacology. Methods Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to determine the ingredients of CLMD. The targets of these components were predicted and screened using the TCMSP, SuperPred, and SwissTargetPrediction databases. The disease targets related to depression were obtained from the GeneCards, OMIM, and DisGeNET databases. The protein-protein interaction (PPI) networks of CLMD and depression were constructed using the STRING database, and Origin 2022 software was used to identify cross-targets by screening the core-acting targets. A network diagram was constructed using Cytoscape3.7.2 software. The DAVID database was used for GO and KEGG enrichment analysis of the core genes. Then, molecular docking was performed to verify the results using AutoDock 4.2.6, AutoDock Tools 1.5.6, PyMOL2.3.0, and Ligplot V 2.2.4 software. Molecular dynamics simulation was performed by iNODS. Finally, a series of in vitro experiments were performed to examine the function of baicalin in corticosterone-stimulated HT22 cells. Results A total of 1011 components in CLMD were detected, and 778 targets were identified through database screening. Key active ingredients included baicalin, luteolin, rhein, etc . A total of 472 targets were screened based on the degree values, among which there were 3 core target genes: PIK3R1, AKT1, and MAPK1. There were many enriched significant pathways, including PI3K/AKT and MAPK pathways. Baicalin binds well to the three core proteins PIK3R1, AKT1, and MAPK1. Baicalin inhibited corticosterone-induced depression-like neuronal injury through activating PI3K/AKT and MAPK/ERK pathways. Conclusion These results suggested that the antidepressant effects of CLMD could be achieved by its active ingredient baicalin, which targeted PIK3R1, AKT1, and MAPK1 to affect PI3K/AKT and MAPK/ERK pathways. This study provided a basis for further in-depth study on the therapeutic mechanism of CLMD in depression.
研究领域:
药理学
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中医学
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神经科学
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分析化学
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计算生物学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2022
·Vol. 50
·期 11
·100146
·
IF 1.193
Chen-Chen MAO,Qi HU,Yi-Ming NING,Da-Jin ZHANG,Yu-Kun GAO,Xiang-Yan XU,Yu-Dong WANG,Mei-Yun SHI,Qiang MA,Hong-Yu XUE
Background and objectives Jiu Wei decoction (JWD) is a traditional Chinese medicine (TCM) applied to control rheumatoid arthritis (RA). However, the active ingredients of JWD are still unclear. In this study, bioinformatics and ultra-high-performance liquid chromatography coupled with mass spectrometry (UPLC-MS/MS) were used to uncover the material basis and mechanism of JWD against RA. Materials and methods Ingredients and potential targets of herbs in JWD were collected from online databases. Then, these targets and RA related targets were overlapped, and the overlapped targets were subjected to further PPI (protein-protein interaction, PPI) analysis. Drug-component-target network and molecular docking were used to predict the potential active compounds. UPLC-MS/MS method was applied to detect and quantify these compounds in JWD alcohol extract. Results A total of 90 constituents and 520 targets were collected from JWD and 95 potential targets were overlapped by comparing these targets with RA-related targets. PPI network analysis indicated that TNF-α, IL-6, AKT1 and VEGFA were the main targets of JWD to RA, and the pharmacological mechanism of these targets might be related to anti-inflammation, pro-apoptosis, and inhibition of angiogenesis. Drug-component-target network and molecular docking results showed that quercetin , wogonin , baicalein , ferulic acid , kaempferol and berberine might be the potential compounds of JWD to RA. Through UPLC-MS/MS analysis, four compounds including quercetin, wogonin, baicalein, and berberine were detected and quantified. Moreover, among the four ingredients, quercetin, wogonin, berberine and baicalein accounted for 15.85%, 25.84%, 15.01%, and 43.3%, respectively. Conclusion Collectively, our study proved that bioinformatic analysis combined with UPLC-MS/MS is a promising method to explore the material basis of anti-RA in JWD and the method is also applicable to other TCMs. The effect of the active compounds still needs further experimental verification in the disease cell model.
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DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Ming Lv,Haidong Gao,Shucai Gao,Yeru Liu,Jingjing Yao,Hongwei Liu,Fayun Wang,Mantang Chen,Lianjun Song,Tianlin Wang,Libin Wan
Ochratoxin A (OTA) contamination in food crops has sparked global concern for potential health risk to humans and animals. A simple, accurate and sensitive method was developed for the determination of OTA using electromembrane extraction (EME) coupled with ultra-high performance liquid chromatography-fluorescence detector (UPLC-FLD). By optimizing the important factors, EME showed good performance for the extraction of OTA in spiked corn flour sample. Using the developed EME-UPLC-FLD method, good linearity for OTA was achieved in the range of 1–100 ng mL −1 , and the limit of detection (LOD) was 0.02306 μg kg −1 , which was well below the established maximum residue limit. The proposed method was successfully applied for the determination of trace OTA in multiple matrices (corn flour extract, corn flour slurry and soybean milk) with satisfactory recoveries ranging from 80.01 % to 116.7 % at four spiked levels, which has great potential to analyze trace chemical contaminant in food crops.
研究领域:
食品安全
·
分析化学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2016
·Vol. 54
·期 7
·1213-1219
·
IF 1.32
Liang Xianrui,Zhao Cui,Su Weike
A novel fingerprinting approach was developed by means of ultra-high-performance liquid chromatography with photodiode array detector (UPLC-PAD) for the quality control of Chimonanthus salicifolius (C. salicifolius). All UPLC analyses were carried out on a Waters Acquity BEH Phenyl column (2.1 × 50 mm, 1.7 μm particle size) at 48°C, with a gradient mobile phase composed of 0.1% aqueous phosphoric acid and acetonitrile at a flow rate of 0.2 mL/min. The method validation results demonstrated the developed method possessing desirable precision [<0.88% relative standard deviation (RSD)], reproducibility (<1.87% RSD), stability (<1.42% RSD) and allowing fingerprint analysis in one chromatographic run within 21 min. The quality assessment was achieved by using chemometrics methods including similarity analysis, hierarchical clustering analysis and principal component analysis. The developed method can be used for further quality control of C. salicifolius.
研究领域:
植物学
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药学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Chongzhen Sun,Xin Tang,Yao Ren,Erpei Wang,Lei Shi,Xiyang Wu,Hui Wu
Neutrase-hydrolysates hydrolyzed from mulberry leaf proteins were separated by ion exchange chromatography, gel filtration chromatography, and semipreparative reverse-phase HPLC. Purified fractions were analyzed for their radical scavenging activity, hemolysis inhibition ability, and cellular antioxidant activity (CAA). Three new antioxidant peptides, P1 (SVL, 317 Da), P2 (EAVQ, 445 Da), and P3 (RDY, 452 Da), were obtained from the most active HPLC fraction (R1) and identified using UPLC-QTOF-MS. These three peptides were then synthesized, and their antioxidant activities were analyzed. P1 and P2 had no ability to inhibit hemolysis of erythrocytes but did show antioxidant activity on HepG2 cells. P3 showed the highest hemolysis inhibition ability (92%) and CAA value (2204 μM QE/100 g peptide). The Tyr residues at the C-terminal region play an important role in the antioxidant activity in P3. Thus, the natural peptide R1 and synthesized P3 could be used as antioxidants and might be promising components of functional foods.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Food & Function
·
2025
·
IF 5.4
Jiapan Gan,Jiayan Xie,Xin Xu,Jiahao Ai,Sisi He,Jiafen Zou,Qiang Yu,Hongli Jin,yanfang liu,Xinmiao Liang
ZhangTouHong (ZTH), highly valuable edible-medicinal resource rich in bioactive flavonoids, lack of chemical composition and biological activities studies. In this work, a preparation process was developed to prepare flavonoid multi-components (FMC) from ZTH, which showed high efficiency and specificity, and increased total flavonoid content by 4.62-fold. Based on UPLC- Q-TOF- MS/MS and molecular networks, the FMC in ZTH were identified for the first time, with a total of 48 flavonoid compounds identified, including 3 potential new compounds. Moreover, the verification results of anti-aging activity demonstrated that FMC have excellent antioxidant activity and anti-aging capacity, extending nematodes lifespan by 21.65% and survival time of oxidative stress nematodes by 35.71%. In summary, this study established an efficient and scalable preparation process for preparing bioactive flavonoid components, and for the first time verified the anti-aging effect of ZTH FMC, providing the scientific basis for deep development and industrial application of this characteristic citrus resource.
研究领域:
天然产物化学
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药用植物学
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分子生物学
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抗衰老研究
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食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）