BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Yunzhi Han,Min Zhou,Mengjiao Zhu,Wei Zhang,Yu Liu,Tao Liu,Zhenyu Chu,Wanqin Jin
miRNA-25 is a critical and specific biomarker for the early diagnosis of pancreatic cancer. However, its sensitive and efficient detection remains challenging due to its extremely low abundance and time-consuming procedures of conventional methods. In this work, we proposed an ultrasensitive electrochemical aptasensor for rapid determination of miRNA-25 within 30 minutes using cubic Co@CNTs nanoaggregates (CC-NAs) as the signal nano-amplifier. This unique nanoarchitecture was in situ constructed through precisely controlled pyrolysis of CoCo Prussian blue analogues as a single precursor. The resulting CC-NAs exhibit excellent conductivity, electrocatalytic activity, and abundant binding sites for immobilizing capture DNA, thereby enhancing the recognition of reverse-transcribed DNA (tDNA) corresponding to miRNA-25. The aptasensor achieved an ultralow detection limit of 0.3 fM (3×10 -16 M) and an ultrawide linear range from 1 fM to 10 nM, along with high reliability and accuracy in real sample analysis. This CC-NAs based aptasensor holds great promise for facilitating rapid and ultrasensitive early screening and warning of various cancers, providing a powerful technique for aiding clinical diagnosis across diverse scenarios.
研究领域:
生物分析化学
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纳米技术
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肿瘤学
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生物传感器
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分子诊断
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Tong Yu,Jingyi Gong,Ping Pang,Jun Ma,Mengjia Wang,Jiahao Ding,Xinjie Zhou,Haoshu Wang,Zhiyi Yin,Gulizhaer Ainiwa,Xiuni He,Shuxia Jiang,Damin Ding,Huixia Xuan,Yu Luo,Kaiyang Wang,Hongli Shan
Despite advances in reperfusion therapy, treatment outcomes for myocardial infarction (MI) remain limited by poor targeting specificity and insufficient bioavailability of therapeutic agents in the ischemic myocardium. Here, we report CCTP-miR@CTP, a non-liposomal nanozyme platform that integrates cardiomyocyte-targeted delivery, intrinsic catalytic antioxidant activity, and therapeutic miRNA delivery within a single multifunctional system. In vitro, CCTP loaded with miR-26a mitigated H₂O₂-induced ROS accumulation, restored mitochondrial membrane potential, and suppressed autophagy and apoptosis in cardiomyocytes. In vivo, CCTP-miR@CTP preferentially accumulated in the infarcted myocardium, where it reduced infarct size, improved cardiac function, suppressed inflammatory responses, promoted angiogenesis, and attenuated extracellular matrix deposition and myocardial fibrosis. Mechanistically, CCTP not only served as an effective nucleic acid carrier but also coordinated redox homeostasis and cell survival pathways, amplifying the therapeutic efficacy of miRNA-based intervention. This study presents a material-design strategy to improve miRNA druggability in cardiovascular medicine and identifies CCTP-miR@CTP as a candidate nanotherapeutic for MI.
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PubMed
高影响因子
Advanced Healthcare Materials
·
2026
·
IF 11
Xiaohan Chen,Peng Song,Yihao Wang,Chuanquan Wang,Liyun Ding,Xi Chen,Ning Wang
Osteosarcoma is a malignant bone tumor for which diagnosis is critical to patient prognosis. MicroRNA-588 is downregulated in osteosarcoma, highlighting its potential as a diagnostic biomarker. However, its low abundance in body fluids makes sensitive detection difficult. This study presents a label-free reflective optical fiber surface plasmon resonance (SPR) biosensor with optimized probe immobilization for ultrasensitive miRNA-588 detection in serum. The sensor utilizes plasmonic coupling between a gold film and gold nanoparticles to enhance the SPR response. By incorporating an A 15 anchor and a T 5 spacer into a single oligonucleotide, the A 15 T 5 probe promotes a more upright presentation of the recognition sequence while simplifying interface construction. Among the configurations evaluated, the A 15 T 5 probe showed the best performance for miRNA-588 detection. The biosensor exhibits a wide detection range from 10 −15 to 10 −7 M, with a low detection limit (LOD) of 1.12 × 10 −17 M, along with good stability and repeatability. In clinical sample testing, the sensor achieved an area under the receiver operating characteristic (ROC) curve of 0.95 and showed greater diagnostic discrimination than serum alkaline phosphatase in the evaluated cohort. This platform enables high-sensitivity detection of trace biomarkers, supporting the diagnosis of major diseases.
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DOI
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·44359
·
IF 3.9
Zhang Yuepeng,Sun Yuhua,Gong Xueshan,Zhao Shuang,Cao Weiwei,Wang Hongbo,Wang Hao,Zhang Xi,Du Changwei,Chen Zhiguo,Deng Lu
Sequence-specific microRNAs (miRNAs) represent promising biomarkers for tumor diagnosis, while current detection methods are often limited by stringent temperature requirements and excessive protease dependency. Herein, we present an isothermal, protein-free cascaded amplification circuit for the sensitive analysis of miRNA-21. This strategy, termed CHA-DNAzyme, integrates a miRNA-activated catalytic hairpin assembly (CHA) reaction with a deoxyribozymes (DNAzyme) component. Specifically, the target miRNA-21 initiates the CHA process via a toehold-mediated strand displacement reaction. This reaction unveils a previously sequestered DNAzyme sequence embedded within a hairpin structure, forming an active double-stranded complex. Subsequently, in the presence of Mg²⁺ cofactors, the catalytically activated DNAzyme drives the continuous cleavage of fluorescent reporter hairpins, releasing a measurable fluorescent signal. Implemented in a one-pot format, the CHA-DNAzyme strategy demonstrates excellent sensitivity (with a limit of detection of 8.70 pM), high specificity (the capability to discern single-base mismatches), short sample-to-answer time (approximately 60 min), and robust performance in analyzing serum samples. This approach holds significant potential to facilitate the advancement of miRNA detection tools and support their application in tumor diagnosis and prognosis assessment.
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DOI
PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
Shuo Li,Xiang-Yu Pei,Xin-Yi Liu,Shu-Liang Wang,Wen Xu,Jing-Jing Wang,Zhen Feng,Han Ding,Yin-Feng Zhang,Rui Zhang
MicroRNAs (miRNAs) are small noncoding RNAs of 18–25 bases. miRNAs are also important new biomarkers that can be used for disease diagnosis in the future. Studies have shown that miR-124 levels are significantly elevated during acute myocardial infarction (AMI) and play a key role in the cardiovascular system. A variety of methods have been established to detect myocardial infarction-related miRNAs. However, most require complex miRNA extraction and isolation, and these methods are virtually undetectable when RNA levels are low in the sample. It may lead to biased results. Thus, it is necessary to develop a technique that can detect miRNA without extracting it, which means that intracellular detection is of great significance. Here, we improved the traditional silicon spheres and obtained a biosensor that could effectively capture and detect specific noncoding nucleic acids through the layer-by-layer assembly method. The sensor is protected by hyaluronic acid so it can successfully escape the lysosome into the cell and achieve detection. With the help of a full-featured microplate reader, we determined that the detection limit of the biosensor could reach 1 fM, meeting the needs of intracellular detection. At the same time, we prepared an oxidative stress cardiomyocyte infarction model and successfully captured the overexpressed miR-124 in the infarcted cells to achieve in situ detection. This study could provide a new potential tool to develop miRNAs for sensitive diagnosis in AMI, and the proposed strategy implies its potential for biomedical research.
研究领域:
分子生物学
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生物医学工程
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心脏病学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Shiwei Xu,Guofeng Wang,Yueyue Feng,Juanjuan Zheng,Liying Huang,Jiahao Liu,Yisha Jiang,Yajun Wang,Nannan Liu
MicroRNAs (miRNAs) are endogenous and noncoding single-stranded RNA molecules with a length of approximately 18–25 nucleotides, which play an undeniable role in early cancer screening. Therefore, it is very important to develop an ultrasensitive and highly specific method for detecting miRNAs. Here, we present a bottom-up assembly approach for modifying glass microtubes with silica nanowires (SiNWs) and develop a label-free sensing platform for miRNA-21 detection. The three-dimensional (3D) networks formed by SiNWs make them abundant and highly accessible sites for binding with peptide nucleic acid (PNA). As a receptor, PNA has no phosphate groups and exhibits an overall electrically neutral state, resulting in a relatively small repulsion between PNA and RNA, which can improve the hybridization efficiency. The SiNWs-filled glass microtube (SiNWs@GMT) sensor enables ultrasensitive, label-free detection of miRNA-21 with a detection limit as low as 1 aM at a detection range of 1 aM–100 nM. Noteworthy, the sensor can still detect miRNA-21 in the range of 102–108 fM in complex solutions containing 1000-fold homologous interference of miRNAs. The high anti-interference performance of the sensor enables it to specifically recognize target miRNA-21 in the presence of other miRNAs and distinguish 1-, 3-mismatch nucleotide sequences. Significantly, the sensor platform is able to detect miRNA-21 in the lysate of breast cancer cell lines (e.g., MCF-7 cells and MDA-MB-231 cells), indicating that it has good potential in the screening of early breast cancers.
研究领域:
生物医学工程
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分子生物学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Bioengineered
·
2021
·
IF 3.269
Yute Sun,Zuoqiong Hou,Binlin Luo,Chujun Li,Jinfang Liu,Jianlan Liu,Jian Tang,Gang Yao
To identify how circular RNA circRNA_0082835 impacts melanoma cells and lymphatic metastasis to observe whether it exerts effects through its action mechanism of sponging microRNA miR-429. Clinical baseline information was collected, and clinical samples were used for detection on circRNA_0082835 and EZH2. The expression of circRNA_0082835, EZH2, and miR-429 was detected by quantitative real-time PCR (RT-qPCR). Cell proliferation was tested with cell counting kit-8 (CCK-8). Flow cytometry was applied to examination of cell cycle levels. Cell invasion and migration were observed by transwell and wound healing. The expression of Wnt/β-catenin pathway, cell cycle and epithelial–mesenchymal transition (EMT) marker proteins was analyzed by western blot. Dual-luciferase determined the binding of miR-429 and circ_0082835. As a result, the expression of circRNA_0082835 was increased and that of miR-429 was decreased with the increase in lymphatic metastasis level. CircRNA_0082835 expression was downregulated by circ_0082835 interference, upregulated by EZH2 interference and also downregulated after transfection of both shRNA-circ_0082835 and shRNA-EZH2. Inhibiting circ_0082835 and EZH2 suppressed the proliferation, invasion and migration, regulated the cell cycle levels, inhibited Wnt/β-catenin and attenuated EMT in melanoma cells. Inhibition of circ_0082835 and/or EZH2 elevated miR-429 expression. The binding among miR-429 and circ_0082835 was verified. MiR-429 inhibitor reversed the effect of circ_0082835 interference while having no significant impact on EZH2. In conclusion, circRNA_0082835 sponges miR-429 to affect the anti-tumor effect of miR-429 in primary melanoma and lymphatic metastasis.
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PubMed
高影响因子
Advanced Healthcare Materials
·
2020
·Vol. 9
·期 11
·1901783
·
IF 7.367
Ting Hong,Yazhong Wei,Xiaomei Xue,Yongyong Li,Haiqing Dong,Xiaoyu Guo,Xueyin Shi,Bin He
Great progress has been made in miRNA nanodelivery for the treatment of myocardial infarction (MI). However, miRNA nanodelivery within the infarct is impeded by microvascular obstruction as a local circulatory disorder caused by microthrombus formation in microvessels. Knowing that low molecular weight heparin (LMWH) can effectively prevent microthrombus formation in microcirculation, it is hypothesized whether surface modification of the nanocarrier with LMWH can overcome microvascular obstruction in the infarct area for better miRNA delivery. Herein, a novel nanocomlex consisting of dendrigraft poly- l -lysine (DGL)-loaded miR-1 inhibitor as the core to decrease apoptosis of cardiomyocytes and LMWH as the shell to overcome microvascular obstruction of the infarct area is developed. The results show that this anti-coagulative nanocomlex is able to reduce microthrombus formation in microvessels and inhibit blood-coagulation factor Xa, thereby overcoming microvascular obstruction in the infarct area. In addition, it further enhances the uptake of miR-1 inhibitor within the infarct and decreases myocardiocyte apoptosis, thus improving the cardiac function and attenuating the myocardial fibrosis. In conclusion, modification of DGL-loaded miR-1 inhibitor with LMWH helps overcome microvascular obstruction in delivering the drug to the infarct area, thus providing a promising therapeutic strategy for achieving a better therapeutic outcome of MI.
研究领域:
纳米技术
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心脏病学
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分子生物学
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药学
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DOI
PubMed
高影响因子
TISSUE & CELL
·
2021
·
IF 2.466
Jian Wang,Jianming Mo,Yuancai Xie,Chunguang Wang
MiR-216b is ectopically expressed in various cancers. Ultrasound microbubbles (UTMBs) are an effective method for miRNA delivery. This article mainly explored the involvement of lncRNA in the effects of UTMBs-mediated miR-216b on non-small cell lung cancer (NSCLC) progression. Expressions and relationship of miR-216b and MALAT1 were examined using quantitative real-time polymerase chain reaction (qRT-PCR), Pearson , TargetScan, and dual-luciferase reporter assay. After the transfection with liposome- or UTMBs-mediated miR-216b mimic (M) or MALAT1 overexpression plasmid alone or together, levels of miR-216b and MALAT1, cell biological behaviors, as well as expressions of apoptosis- and epithelial mesenchymal transition (EMT)-related markers were examined using qRT-PCR, cell functional experiments, and western blot. Besides, we used qRT-PCR to quantify the expressions of multiple downstream miRNAs of MALAT1. MiR-216b expression was weakened yet MALAT1 expression was enhanced in NSCLC tissues, and miR-216b was negatively bound to MALAT1. TargetScan analysis manifested that miR-216b, targeted by MALAT1, was down-regulated in NSCLC cells. UTMBs-mediated miR-216b M further intensified miR-216b level yet weakened cell biological behaviors. The inhibitory effect of UTMBs-mediated miR-216b M on cell biological behaviors and MALAT1 expression was greatly better relative to that of miR-216b M. Moreover, miR-216b restrained the cell biological behaviors by repressing MALAT1 expression. We further manifested that miR-216b facilitated the expressions of apoptosis-related markers, but restrained those of EMT-related markers by repressing MALAT1 expression. Moreover, UTMBs-mediated miR-216b M enhanced the expressions of downstream multiple miRNAs of MALAT1, but this tendency was reversed by co-transfection of overexpressed MALAT1 and miR-216b M. Collectively, UTMBs-mediated miR-216b M restrained NSCLC cell growth by modulating the MALAT1-miRNA axis.
研究领域:
肿瘤学
·
分子生物学
·
医学超声波技术
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产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 4.446
Ping Ouyang,Yang Qing,Shuhao Zou,Chenxin Fang,Jialun Han,Yuxing Yang,Haiyu Li,Zhencui Wang,Jie Du
It is of great significance to develop a sensitive detection platform for miRNA , a non-invasive biological indicator for the diagnosis, treatment and prognosis monitoring of tumors. In this paper, we design an electrochemical DNA sensor to detect miR-122, based on toehold promoted strand displacement reaction, combined with enzyme assisted cycle amplification. In this strategy, when miR-122 and Exonuclease III (Exo III) coexist in the detection system, the toehold promotes the chain replacement reaction, and the miR-122 hybridizes with a stem-loop DNA, HC-DNA. Simultaneously, Exo III hydrolyzes part of HC-DNA and releases miR-122 and sign-DNA. Then, after multiple enzymatic cyclic amplification reactions (N Cycle), a large amount of Sign-DNA was released. Methylene blue (MB), as the signal molecule, is attached to sign-DNA, close to the surface of the electrode, and resulting in a increased electrochemical signal. Using this method, we can qualitatively analyze the concentration of miR-122 by the amplified electrochemical signals. The detection limit of miR-122 is 0.304 pM, lower than that of the many common large-scale instrumental analysis. Further, the method has good selectivity and anti-interference performance in complex environments. This electrochemical DNA biosensor has potential applications in highly sensitive detection of target molecules even concluding viruses.
研究领域:
生物化学
·
分子生物学
·
分析化学
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DOI
高影响因子
ANALYST
·
2018
·Vol. 143
·期 13
·3134-3140
·
IF 3.864
Xiaotong Wei,Xiaolei Duan,Xiaoyan Zhou,Jiangling Wu,Hongbing Xu,Xun Min,Shijia Ding
Herein, a dual channel surface plasmon resonance imaging (SPRi) biosensor has been developed for the simultaneous and highly sensitive detection of multiplex miRNAs based on strand displacement amplification (SDA) and DNA-functionalized AuNP signal enhancement. In the presence of target miRNAs (miR-21 or miR-192), the miRNAs could specifically hybridize with the corresponding hairpin probes (H) and initiate the SDA, resulting in massive triggers. Subsequently, the two parts of the released triggers could hybridize with capture probes (CP) and DNA-functionalized AuNPs, assembling DNA sandwiches with great mass on the chip surface. A significantly amplified SPR signal readout was achieved. This established biosensing method was capable of simultaneously detecting multiplex miRNAs with a limit of detection down to 0.15 pM for miR-21 and 0.22 pM for miR-192. This method exhibited good specificity and acceptable reproducibility. Moreover, the developed method was applied to the determination of target miRNAs in a complex matrix. Thus, this developed SPRi biosensing method may present a potential alternative tool for miRNA detection in biomedical research and clinical diagnosis.
研究领域:
生物传感技术
·
分子诊断
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分析化学
·
医学检测
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Yun Chen,Dehui Qiu,Xiaobo Zhang,Yuan Liu,Mingpan Cheng,Jianping Lei,Jean-Louis Mergny,Huangxian Ju,Jun Zhou
G-quadruplex/hemin (G4/hemin) DNAzymes are biosensing systems, but their application remains limited by an overall low activity and a rather high level of unwarranted background reactions. Here, these issues were addressed through the rational design of F3T-azaC-hemin, a G4-based construct in which the hemin is covalently linked to the G4 core and its binding site flanked with a nucleotide activator, here d(T-azaC). This design led to a G4-DNAzyme whose performances have been ca. 150-fold increased compared to the parent G4-based system. The utility of F3T-azaC-hemin was demonstrated here through the ultrasensitive chemiluminescent detection of miRNA-221. The limit of detection (LOD) has been decreased to the femtomolar range, making it a new and highly efficient molecular tool in the biosensing technology field.
研究领域:
生物传感技术
·
分子生物学
·
分析化学
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DOI
PubMed
高影响因子
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