ACS Biomaterials Science & Engineering
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2023
·
IF 5.8
Zhengjiang Xu,Yuan Zhang,Danping Lu,Guofang Zhang,Yang Li,Zufu Lu,Fei Wang,Guocheng Wang
Accumulation of senescent cells in the elderly impairs bone homeostasis. It is important to alleviate cell senescence and scavenge excessive oxidative stress for enhanced bone fracture healing in elderly patients. In this study, resveratrol (RSV), an antioxidant drug, was encapsulated in a biocompatible zeolitic imidazolate framework-8 (ZIF-8) nanoparticle to protect it from oxidation and improve its bioavailability. Cells responsible for bone healing, including osteoblasts, bone marrow-derived mesenchymal stem cells (BMSCs), macrophages, and endothelial cells, were used to evaluate the regulatory role of the nanoformulation in the alleviation of cellular senescence and promotion of cell functions. It was proved that the nanoformulation sustainably released RSV with well-preserved bioactivity and improved bioavailability. Cell experiments confirmed that ZIF-8/RSV was capable of alleviating the senescence of cells [human osteoblasts (HOBs), BMSCs, H2O2-induced senescent vascular endothelial cells (HUVECs)] and scavenging excessive intracellular reactive oxygen species (ROS). Excitingly, the ZIF-8/RSV improved the osteogenic ability of senescent osteoblasts and promoted macrophage M2 polarization. In addition, the ZIF-8/RSV also enhanced the angiogenic function of senescent HUVECs. More importantly, the ZIF-8/RSV nanoformulation outperformed the REV alone, indicating the critical role of encapsulation using ZIF-8. These findings suggest that the ZIF-8/RSV nanoformulation exhibits potential for bone fracture treatment in elderly patients.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Licong Yang,Yabin Wang,Guodong Zheng,Zhiwei Li,Jingtao Mei
Resveratrol (Res) is a common natural polyphenol that inhibits inflammation and oxidative stress in Alzheimer's disease (AD). However, the absorption efficiency and in vivo bioactivity of Res are poor. High fat diet-induced metabolic disorders, including obesity and insulin resistance, can promote AD-related β-amyloid (Aβ) aggregation, Tau protein phosphorylation and neurotoxicity. Gut microbiota play a role in modulating metabolic syndrome and cognitive impairment. Herein, flower-like Res-loaded selenium nanoparticles/chitosan nanoparticles ( [email protected] @Res-CS-NPs) with higher loading capacity (64 %) were prepared to regulate gut microbiota in cases of AD with metabolic disorder. The nano-flowers could restore gut microbiota homeostasis to reduce lipopolysaccharide (LPS) formation and LPS-induced neuroinflammation. Additionally, [email protected] @Res-CS-NPs can prevent lipid deposition and insulin resistance by decreasing Firmicutes levels and increasing Bacteroidetes levels in the gut, further inhibiting Aβ aggregation and Tau protein phosphorylation through the JNK/AKT/GSK3β signaling pathway. Moreover, [email protected] @Res-CS-NPs treatment was able to regulate the relative levels of gut microbiota associated with oxidative stress, inflammation and lipid deposition, including Entercoccus , Colidextribacter , Rikenella , Ruminococcus , Candidatus_Saccharimonas , Alloprevotella and Lachnospiraceae_UCG-006 . Overall, [email protected] @Res-CS-NPs significantly enhances cognitive ability in AD mice with metabolic disorder, highlighting their potential for preventing cognitive impairments in AD.
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Polymer Chemistry
·
2023
·Vol. 14
·期 14
·1665-1679
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IF 4.6
Jiaming Liu,Xiaohong Liu,Xiaohua Cui,Jingjing Qin,Mengqin Wu,Lifu Fu,Min Shi,Dingsong Wang,Liyan Liang
Using biomass resources to prepare thermosets seems to have become a trend in the future of a sustainable society, and an active ester may be an excellent epoxy hardener for the electronic packaging field. In this work, we synthesized a resveratrol-derived epoxy (REP) and two resveratrol-derived active ester hardeners (triacetyl resveratrol (TAR), tri(m-toluoyl) resveratrol (TTR)), and then two resveratrol-derived bio-based epoxy thermosets (REP/TAR, REP/TTR) were prepared by the curing reaction of the epoxy and the hardener. The properties and structures of the thermosets were characterized and investigated. Results showed that the properties of REP/TAR in all aspects were better than those of REP/TTR; to be specific, the REP/TAR exhibited a higher gel fraction and better solvent resistance, indicating its better cross-linked network; the glass transition temperature of REP/TAR was higher and reached 177 °C (DMA) and the thermal stability of TEP/TAR was also better and the char yield at 600 °C was 46.5%. The main reason could be that the steric hindrance of acetyl groups in REP/TAR was smaller than that of m-toluoyl groups in REP/TTR. What's more, a novel analytical method was proposed for use in thermogravimetric analysis (TGA) to analyze the degradation process of thermosets, revealing a degradation process that was unique to the active ester cured epoxy thermosets. In all, the resveratrol-derived biobased epoxy thermosets could be a promising candidate for replacing fossil-derived thermosets and have potential application in the electronic packaging field, and the study on active ester hardeners might inspire the design and synthesis of subsequent active ester hardeners.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Ping Shao,Jieru Feng,Peilong Sun,Christos Ritzoulis
This work studied the effects of different concentrations of gum arabic (GA) on the interfacial adsorption kinetics of whey protein (WP) at the oil–water interface, the possibility of emulsion stabilized by WP/GA mixtures, and use of such emulsions as resveratrol delivery systems. Dynamic interface pressure measurements indicated that the change of GA concentration significantly affected the diffusion, penetration and rearrangement of WP at the oil–water interface, while measurements of hydrodynamic diameter and ζ-potential indicated electrostatic and hydrophobic interactions between WP and GA. The rheological measurements of the emulsions supported the above observations and indicated that different GA concentrations affected the viscoelastic properties of the emulsion. Adding resveratrol into oil influenced the adsorption of WP at the oil–water interface, thus affecting the emulsion stability and the encapsulation efficiency of resveratrol. The emulsions stabilized by WP and WP/GA mixtures have encapsulation efficiencies of >50% for resveratrol, while emulsions showed the best stability at a GA concentration of 0.2%. These results indicate that GA could effectively improve the emulsifying capacity of WP, while the emulsions prepared by WP/GA mixtures have the potential to serve as carriers for bioactive components.
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JOURNAL OF MEMBRANE BIOLOGY
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2017
·Vol. 250
·期 5
·483-492
·
IF 1.696
Chai Ran,Chen Yafei,Yuan Hongbo,Wang Xuzhao,Guo Shuai,Qi Jinlong,Zhang Hailin,Zhan Yong,An Hailong
Calcium-activated chloride channels (CaCCs) play vital roles in a variety of physiological processes. Dysfunction of the CaCCs is implicated in many diseases. Drug discovery targeting at CaCCs has recently become possible with the determination that TMEM16A is the molecular identity of CaCCs. In this study, we demonstrated that resveratrol (RES), a Chinese traditional medicine compound, is a novel activator of TMEM16A. The yellow fluorescence protein quenching assay and measurement of intracellular calcium fluorescence intensity show that RES activates TMEM16A channels in an intracellular Ca 2+ -independent way. The data of inside-out patch clamp revealed that RES dose-dependently activates TMEM16A (EC 50 = 47.92 ± 9.35 μM). Furthermore, RES enhanced the contractions of the ileum of guinea pigs by activating the TMEM16A channel, which indicated that RES might be a promising drug for the treatment of gastrointestinal hypomotility. As RES was able to induce TMEM16A channel activation, TMEM16A can be added to the list of RES drug targets.
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CELLULAR SIGNALLING
·
2016
·
IF 4.191
Wenjun Zhao,Aiyun Li,Xin Feng,Ting Hou,Kang Liu,Baolin Liu,Ning Zhang
This study aims to investigate the effects of metformin and resveratrol on muscle insulin resistance with emphasis on the regulation of lipolysis in hypoxic adipose tissue. ICR mice were fed with high fat diet (HFD) for 10 days with administration of metformin, resveratrol, or intraperitoneal injection of digoxin . Adipose hypoxia, inflammation and cAMP/PKA-dependent lipolysis were investigated. Moreover, lipid deposition and insulin resistance were examined in the muscle. Metformin and resveratrol attenuated adipose hypoxia, inhibited HIF-1α expression and inflammation in the adipose tissue of HFD-fed mice. Metformin and resveratrol inhibited lipolysis through prevention of PKA/HSL activation by decreasing the accumulation of cAMP via preserving PDE3B . Metformin and resveratrol reduced FFAs influx and DAG accumulation, and thus improved insulin signaling in the muscle by inhibiting PKCθ translocation. This study presents a new view of regulating lipid metabolism to ameliorate insulin resistance and provides the clinical guiding significance for obesity and type 2 diabetes with metformin and resveratrol treatment.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Yanli Fu,Peipei Zhu,Hong Lin,Chun Hu,Lijuan Han,Weinong Zhang,Junbo He
The poor solubility and stability of resveratrol (Res) represent significant obstacles to its utilization and efficacy. Encapsulating in sodium caseinate (NaCas) has also faced issues concerning low encapsulation efficiency and stability. This study presented the synthesis of a novel NaCas and dialdehyde β-cyclodextrin (DA-β-CD) conjugate through the Schiff base reaction using a wet-heating method, which was then utilized to encapsulate resveratrol. Results showed the DA-β-CD was formed in the form of needle-like crystals, whereas the NaCas-DA-β-CD conjugate had a nanosheet structure. The NaCas-DA-β-CD exhibited a substantial increase in surface hydrophobicity, emulsifying activity index, and emulsion stability index. The incorporation of resveratrol into NaCas-DA-β-CD demonstrated a consistent and uniform dispersion at the nanoscale level. It achieved an encapsulation efficiency of 96.8% and exhibited excellent chemical stability, which surpasses those of the NaCas system. Furthermore, the water solubility and antioxidant capacity of resveratrol in NaCas-DA-β-CD exhibited characteristics similar to those in NaCas. The compacted nanosheet structure of the Res-NaCas-DA-β-CD complex, as observed in TEM and SEM, is likely responsible for those enhancements. Hence, the DA-β-CD can serve as an effective polysaccharide for protein modification, while the NaCas-DA-β-CD conjugate can function as an innovative delivery carrier for resveratrol and other hydrophobic bioactives.
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BIOMEDICAL CHROMATOGRAPHY
·
2024
·
IF 1.8
Xiaomin Xu,Donghua Yu,Yu Wang,Peng Xu,Xin Jiang,Fang Lu,Shumin Liu
Resveratrol (Res) has been demonstrated to have beneficial effects on gouty nephropathy (GN). However, the mechanisms of Res on GN remain unclear. This study aimed to investigate the mechanisms of Res on GN. In this study, network pharmacology technology was used to predict the Res targets in the prevention and treatment of GN. Renal metabonomics was used to identify differential metabolites in kidney tissue of GN model rats. Finally, molecular docking technology was used to verify the binding ability of Res to key targets. Metabonomics analysis showed that 24 potentially important metabolites were involved in the prevention and treatment of GN with Res. After exposure to Res, metabolite levels normalized. The network pharmacology analysis showed that 24 key targets were involved in the prevention and treatment of GN disease. According to the metabolite–gene network diagram, we identified two core genes, PTGS1 and PTGS2, and found that both were involved in the arachidonic acid metabolism pathway. Molecular docking further verified the affinity of Res binding to PTGS1 and PTGS2. In conclusion, the mechanism of Res against GN may be the regulation of arachidonic acid metabolism through the regulation of PTGS 1 and PTGS 2.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Xin Yin,Wusigale,Hao Cheng,Paul Van der Meeren,Li Liang
Whey protein isolate (WPI) is mainly composed of β-lactoglobulin (β-LG), α-lactalbumin (α-LA) and bovine serum albumin (BSA). The aim of this study was to compare and analyze the influence of WPI and its three main constituent proteins, as well as proportionally reconstituted WPI (R-WPI) on resveratrol. It was found that the storage stability of resveratrol was protected by WPI, not affected by R-WPI, but reduced by individual whey proteins at 45°C for 30 days. The rank of accelerated degradation of resveratrol by individual whey proteins was BSA > α-LA > β-LG. The antioxidant activity, localization of resveratrol and oxidation of carrier proteins were determined by ABTS, H 2 O 2 assay, synchronous fluorescence, carbonyl and circular dichroism. The non-covalent interactions and disulfide bonds between constituent proteins improved the antioxidant activity of the R-WPI-resveratrol complex, the oxidation stability of the carrier and the solvent shielding effect on resveratrol, which synergistically inhibited the degradation of resveratrol in R-WPI system. The results gave insight into elucidating the interaction mechanism of resveratrol with protein carriers.
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Arabian Journal of Chemistry
·
2024
·
IF 5.3
Ping Cheng,Botao Wang,Shuying Liang,Yuqi Yang,Shixin Gui,Kai Zhang,Yingying Sun,Shaoqi Qu,Lin Li
Colistin used to be regarded as the last-resort treatment for the infection caused by multidrug-resistant gram-negative bacteria. However, the emergence and widespread of mcr-1 seriously reduce the clinical effectiveness of colistin, constituting a serious threat to global public health. Due to the difficulties in the development of new antibiotics, restoring antibiotic susceptibility with adjuvants is undoubtedly a rational option. As naturally occurring compounds, resveratrol and capsaicin could be extracted from many natural plants, due to various antimicrobial properties, they have received significant attention. Herein, the synergistic activity of colistin combined with resveratrol and capsaicin against mcr-1 -positive Escherichia coli were investigated by checkerboard method and time-killing assays. The in-depth molecular mechanisms were elucidated by scanning electron microscopy, fluorescent probe experiments, transcriptome and metabolome analysis. Molecular docking assay was taken to analyse potential interactions between resveratrol/capsaicin and MCR-1. Finally, the in vivo efficacy of combined therapy against mcr-1- positive Escherichia coli was assessed. Our results demonstrated that colistin combined with resveratrol and capsaicin acted synergistic activity against mcr-1- positive Escherichia coli both in vivo and in vitro . Further mechanistic studies showed that the combined therapy could exacerbate cell membrane damage, increase membrane permeability, disrupt the homeostasis of PMF, inhibit ATP synthesis, and efflux pump activity. In addition, the combined therapy could inhibit central carbon metabolism, and reduce tricarboxylic acid cycle and oxidative phosphorylation. Moreover, molecular docking assay revealed resveratrol/capsaicin could bind to MCR-1 stably. Our study indicated that colistin in combination with resveratrol and capsaicin as a novel therapy could provide a trustworthy foundation to establish the treatment plan for mcr-1- positive Escherichia coli.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yanan Gao,Xue Hu,Haiying Zhang,Simin Chen,Jiaqi Yu,Deqiao Dong,Haihe Zhan,Bingmiao Gao,Dingguo Li,Zhenmiao Qin,Yan Liu
Myocardial infarction (MI) is a critical heart disease with a high mortality rate, posing a serious risk to human health. Injectable hydrogels possess considerable therapeutic promise for managing MI through the application of tissue-engineered scaffolds for cell delivery. However, the oxidative stress microenvironment of MI lesions leads to low cell retention and survival. In this study, a thermo-sensitive resveratrol micelles (RES-M) loaded chitosan hydrogel (RM-CH) was developed against oxidative stress injury in cardiomyocytes (CMs). RM-CH had better scavenging ability against DPPH and ABTS radicals compared to industry-standard ascorbic acid. RM-CH had good biodegradability, histocompatibility and cytocompatibility. The injection process of the hydrogel did not cause cell injury. DHE and DCFH-DA staining showed that it can improve the survival of CMs by fighting against the oxidative stress microenvironment. Live/Dead staining of CMs in hydrogels showed that the addition of RES-M could rescue cell survival under oxidative stress. Hence, this work highlights the potential clinical applications of RM-CH which may present an ideal scaffold material for injectable cardiac tissue engineering.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Sufang Chen,Weiwei Cheng,Wenrui Liu,Erna Li,Feng Xiao,Jialiang He,Lili Liu,Suna Han,Baocheng Xu
In this study, zein-peanut isolate protein (PPI) composite nanoparticles were fabricated by pH-driven self-assembly method and their potential applications in Pickering emulsion stabilization and resveratrol encapsulation were systematically explored. The results showed that the particle size, zeta potential, and dispersibility were significantly affected by the Zein:PPI mass ratio. Among them, the composite particles with a mass ratio of Zein:PPI of 1:2 showed the best performance, with an average particle size of approximately 389 nm, a low PDI value (0.32) and a high surface charge (−42 mV), indicating superior colloidal stability. Scanning electron microscopy results showed a porous lamellar structure, and contact angle analysis indicated that the surface wettability of the composite particles increased with increasing PPI ratio. Spectroscopic analyses (UV–vis, fluorescence, circular dichroism and FTIR) revealed significant changes in the protein secondary structure, with a decrease in α-helix and an increase in β-folding, reflecting stronger protein-protein interactions. Molecular docking and molecular dynamics simulations further verified the formation of a stable composite structure between zein and PPI mainly through hydrophobic interactions and hydrogen bonding. The Pickering emulsions stabilized by the composite particles were of the oil-in-water type and exhibited remarkable thermal and long-term storage stability. Meanwhile, they showed good colloidal stability under conditions of a wide pH range and high salt concentration. Rheological tests showed that the emulsions exhibited shear-thinning behavior and predominantly elastic gel properties, reflecting the dense interfacial structure. Furthermore, the composite particles significantly improved the encapsulation efficiency of resveratrol (86.5 %) and enhanced its antioxidant capacity. As a resveratrol-loaded emulsion system, the Pickering emulsion stabilized by Zein-PPI reduced the release rate of free fatty acid (FFA) in in vitro digestion to less than 6 %, and increased the bioaccessibility to more than 40 %. Moreover, the Pickering emulsion stabilized by Zein-PPI was superior to the emulsion stabilized by Zein and PPI in terms of sustained release, anti-ultraviolet stability and simulated gastrointestinal digestion. In summary, zein–PPI composite nanoparticles present a promising platform for the development of stable, lipid-soluble bioactive delivery systems in functional food applications.
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危险品化学品经营许可证（带存储）