BIOMEDICAL CHROMATOGRAPHY
·
2023
·Vol. 37
·期 5
·e5611
·
IF 1.8
Zhiyu Fan,Gangqiang Yang,Xinjie Wu,Yanan Yang,Jinfang Xu
Biflavonoids are naturally occurring compounds consisting of two flavonoid moieties that have received substantial attention from researchers. Although many kinds of biflavonoids are typically distributed in Selaginella uncinata with hypoglycemic effect, their anti- α -glucosidase activities are not yet clear. In this study, a ligand fishing strategy for fast screening of α -glucosidase inhibitors from S. uncinata was proposed. α -Glucosidase was first immobilized on Fe 3 O 4 magnetic nanoparticles (MNPs) and then the α -glucosidase–functionalized MNPs were incubated with crude extracts of S. uncinata to fish out the ligands. Furthermore, considering the similarity and easy confusion of the structures of biflavonoids, the fragmentation patterns of different types of biflavonoids were studied. Based on this, 11 biflavonoids ligands with α -glucosidase inhibitory activities were accurately and quickly identified from S. uncinata with ultra-high-performance liquid chromatography–quadrupole time-of-flight–tandem mass spectrometry. Furthermore, these ligands were confirmed to be potential inhibitors through the in vitro inhibitory assay and molecular docking.
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IONICS
·
2023
·Vol. 29
·期 3
·1187-1197
·
IF 2.8
Zhong Xiaoqiu,Yao Qingyun,Tang Shuangmei,Kong Zhenzhen,Yang Chao,Zang Limin,Qiu Jianhui
Fibrous all-solid-state flexible supercapacitors have attracted much attention in the functional field of wearable electronic devices due to their excellent deformability. It is very important to select suitable flexible substrate, active substance, and binding means to improve electrode performance. In this paper, carbon fiber with high conductivity and the active site was used as a flexible substrate, and FeOOH with high specific capacity was grown on the surface of carbon fiber yarn by one-step in situ electrodeposition. The influence of different deposition conditions on the morphology of FeOOH and the electrochemical performance of the flexible electrode was studied. The electrode obtained by deposition at a constant voltage of 2.5 V for 150 min has a high specific capacitance of 10.44 F cm −3 . In addition, the device has a high voltage window of 1.6 V and excellent cycle stability of 7000 cycles when formed as an all-solid-state symmetric supercapacitor.
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Frontiers in Pharmacology
·
2023
·
IF 5.6
Zhang Xiuying,Chen Qilei,Zhao Jia,Zhao Wei,Fan Ni,Wang Yu,Chen Hubiao,Rong Jianhui
Myocardial infarction (MI) is a highly prevalent and lethal disease worldwide. Prevention and timely recovery are critical for the control of the recurrence and heart failure in MI survivors. The present study was designed to investigate the cardioprotective activity of the herbal medicine formula Baoyuan Decoction (BYD) and identify the active compounds and molecular targets. The ethanolic BYD extract (BYDE) was prepared by water extraction and ethanol precipitation of four herbal medicines, Astragali Radix, Ginseng Radix et Rhizoma, Cinnamomi Cortex, and Glycyrrhizae Radix et Rhizoma. Initially, BYDE was validated for the cardioprotective effectiveness in a mouse model of ischemia injury and rat cardiomyocyte H9C2 cells. As results, BYDE effectively reduced infarct size from 56% to 37% and preserved cardiac functions in mouse MI model while protected H9C2 cells against oxygen glucose deprivation. Subsequent network pharmacology analysis revealed that 122 bioactive ingredients, including flavonoids and saponins from the UPLC-MS/MS profile of BYDE, might target 37 MI-related proteins, including inflammatory and apoptotic mediators (e.g., TNF, NFKB1, CASPs, TNFRSF1A, CXCL12, BCL2A1). Pathway enrichment analysis suggested that BYDE might control the cardiac inflammation via targeting the tumor necrosis factor-alpha (TNF-α)/nuclear factor-κB (NF-κB) pathway while the selected targets were also implicated in IL-17 signaling pathway, lipid and atherosclerosis. Consequently, adenosine, ginsenoside Rh2, isoliquiritigenin, and licochalcone A were selected to generate the four-compound mixture AGILe and validated for the inhibitory effects on the TNF-α/NF-κB pathway. The results of the present study suggested that the mixture AGILe might be a potential cardioprotective remedy against MI.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Tao He,Xuemei Pan,Wenguang Zhou,Huijun Ding,Mengting Liu,Mingxue Xiang,Qian Lou,Liu Han,Yan Zhang,Yixiao Wu,Yiling Chen
Pyrolytic reduction as an effective method is generally employed for synthesizing zero-valent iron @ biochar composite (ZVI@BC). However, it suffers from intractable obstacles, such as poor zero-valent iron (ZVI) dispersibility and low α-iron (α-Fe 0 ) content. Herein, we reported a possible way to target the dilemma for the first time, i.e., adding ammonium bicarbonate (NH 4 HCO 3 ) pretreatment step before pyrolytic reduction. In detail, the special NH 4 HCO 3 not only served as a precipitant to uniformly disperse ZVI over biochar by gradually releasing OH – , but also as a reductant to facilitate γ-Fe 0 transforming to α-Fe 0 in ZVI (∼71 %) via providing sufficient NH 3 . Besides, NH-ZVI@BC 3/1/5 exhibited outstanding oxytetracycline hydrochloride (OTC) removal ability (∼2869.1 mg/g), with the removal efficiency of 99.0 % at the dosage of 0.05 g/L. The removal mechanism was investigated which involved adsorption dominated by complexation, π-π electron donor acceptor interaction, hydrogen bonds, and electrostatic interactions. As well, OTC degradation was achieved by [H] and ·OH resulting from Fe-C micro-electrolysis and Fenton-like reaction during the corrosion of ZVI in ZVI@BC, respectively. In addition, four possible degradation pathways were proposed, and the contribution role in OTC removal followed the sequence: adsorption > degradation involved with [H] > degradation involved with ·OH. Moreover, N-ZVI@Biochar 3/1/5 exhibited easy separability, excellent reusability, and well practical applicability. Overall, this work not only opens a new avenue for the fabrication of ZVI@BC with high ZVI dispersibility and content of α-Fe 0 , but also shed light on the environmental remediation of antibiotics.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Lukai Ma,Yun Cui,Feng Wang,Huifan Liu,Weiwei Cheng,Lianxin Peng,Charles Brennan,Soottawat Benjakul,Gengsheng Xiao
This study aimed to develop and evaluate a method for simultaneously analyzing malondialdehyde (MDA) and two typical toxic α,β-unsaturated aldehydes, 4-hydroxy-2-hexenal (HHE), and 4-hydroxy-2-nonenal (HNE), using ultra-high-performance liquid chromatography with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS). The method possessed widely linear range (10–1000 ng/mL). The limit of detection (LOD) and limit of quantitation (LOQ) of MDA, HHE, and HNE were 2.0 and 5.0 ng/g, 2.0 and 5.0 ng/g, and 0.1 and 0.3 ng/g, respectively; the recovery rates all fall into 95.56–104.22 %. The method was sufficiently precise (<5%), and not affected by the analysis matrix. Application to 17 food products revealed total MDA, HHE, and HNE contents were 0.11–3.56, 0.05–3.32, and 0.09–3.70 μg/g, respectively. It will be useful in future research on the influence of food composition and main substrate structure on the generation and distribution of these three aldehydes and the implementation of corresponding control methods.
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Applied Materials Today
·
2021
·
IF 10.041
Maohua Chen,Yan Hu,Yanhua Hou,Yuting Sun,Maowen Chen,Menghuan Li,Lu Tan,Zhong Luo,Kaiyong Cai
By exploiting the high reactive-oxygen-species(ROS) level in osteoporosis fractured sites, a ROS responsive biomimetic multilayer onto titanium(Ti) implant composed of gelatin(Gel)/dextran amine (DA) and α-melanocyte-stimulating hormone (α-MSH) has been constructed using layer-by-layer spin self-assembled technique, which was further cross-linked with 4-nitrophenyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2) benzyl carbonate (NBC)-grafted-polyethylene glycol (PEG-NBC) for in situ delivery of α-MSH. The PEG-NBC cross-linked Gel/DA multilayer onto Ti substrate (Ti/LBL NBC -MSH) could be broken down when exposed to ROS-rich pathological environment, thus leading to a sustained release of α-MSH from multilayer in a controlled manner. It could maintain the α-MSH concentration at a constant level to promote in situ MSCs differentiation and inhibit osteoclast activity through regulating GSK-3β/β-catenin signal pathways with Runx2 positive feedback regulation loop. Micro-CT analysis, hematoxylin/eosin (HE) staining and Masson's trichrome (MT) staining further confirmed that new bone has fused into mature lamellar bone and trabecularism along the interface of Ti/LBL NBC -MSH in osteoporotic rats. Therefore, this study offers a new perspective on the development of biofunctional Ti implants as supplementary treatment for osteoporotic fracture repair in a clinical context.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2020
·
IF 2.751
Xiang Zhou,Yiqiang Ye,Shasha Liu,Wubin Shao,Liwei Liu,Song Yang,Zhibing Wu
Plant viral diseases, known as “plant cancer”, with high contagiosity can substantially reduce crop quality and yield. To identify potential anti- tobacco mosaic virus (TMV) agents with different mechanisms, a series of novel α-aminophosphonate derivatives containing a chalcone moiety were designed and synthesized. Bioassay results revealed that some target compounds exhibited improved curative activity against TMV in vivo, and the EC 50 value of compound B 3 was 356.7 mg L −1 . The activities of the defensive enzymes POD and CAT from tobacco leaves treated with B 3 and B 17 showed that these target compounds could improve the photosynthetic ability of the leaves and activate plant host resistance against TMV infection. The binding constant between B 3 and TMV Coat Protein (CP) (2.51 × 10 8 M −1 ), calculated by the fluorescence titration experiment and docking results, revealed that B 3 has a strong interaction with TMV CP. Further docking analysis revealed that B 3 was embedded between two layers of the TMV CP, which was consistent with the 2:1 binding mode of TMV CP and B 3 determined by the binding affinity experiment. The TEM morphological study of TMV treated with B 3 and B 17 indicated that this series of target compounds may trigger the disassembly of TMV by interacting directly with TMV CP. This study provides new insight for the discovery of antiviral compounds with two different mechanisms of action.
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JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
·
2019
·
IF 1.479
Wei Zhang,Yee-Kwong Leong,Chunbao Sun,Hong Fan,Xianwei Zhang
The yield stress and zeta potential of washed spherical α-Al2O3 suspensions with two to four layers of critic acid(CA) and polyethyleneimine(PEI_Mw1.8and 70 kDa) adsorbed sequentially were characterized. With CA adsorbed as the first layer, pHξ=0 was shifted to a lower pH and the maximum yield stress (τymax) was reduced. With PEI adsorbed as the first layer, the pHξ=0 was moved slightly higher. With the introduction of the second layer, the CA/PEI additive was formed which functioned as a bridging agent. The pH range of yield stress was broadened significantly and increased with the number of layers. This pH ranged from 2.5 to 11. The CA/PEI multilayer additive loses its positive charge at pH >11. There was less adsorption in layer3 and layer4 when lower Mw PEI is applied to the suspension. The CA/PEI multilayer additive formed by PEI of Mw70000 and CA was larger in size than that formed by PEI of Mw 1800 and CA. Lower Mw PEI produced a stronger flocculated network or higher τymax suspension due to a higher concentration of multilayer additive. CA/PEI multilayer additive was participating in bridging and particle bridging interaction was not affected by the adsorption order of CA and PEI.GRAPHICAL
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Molecular Medicine Reports
·
2015
·Vol. 12
·期 6
·7992-8002
·
IF 1.554
Xu‑Dong Cheng,Jun‑Fei Gu,Jia‑Rui Yuan,Liang Feng,Xiao‑Bin Jia
The migration and invasion of lung cancer cells into the extracellular matrix contributes to the high mortality rates of lung cancer. The protein kinase C (PKC) and downstream signaling pathways are important in the invasion and migration of lung cancer cells. Calycosin (Cal), an effector chemical from Astragalus has been reported to affect the recurrence and metastasis of cancer cells via the regulation of the protein expression of matrix metalloproteinases (MMPs). The inhibition of Cal on the migration and invasion of A549 cells was investigated in the present study. Cell viability and apoptosis assays were performed using MTT and flow cytometric analyses. A wound healing assay and Transwell invasion assay were performed to evaluate the effect of Cal on A549 cell migration and invasion. Invasion‑associated proteins, including MMP‑2, MMP‑9, E‑cadherin (E‑cad), integrin β1, PKC‑α and extracellular signal‑regulated kinase 1/2 (ERK1/2) were detected using western blotting. In addition, PKC‑α inhibitor, AEB071, and ERK1/2 inhibitor, PD98059, were used to determine the association between the suppression of PKC‑α /ERK1/2 and invasion, MMP‑2, MMP‑9, E‑cad and integrin β1. Cal was observed to suppress cell proliferation and induce apoptosis. There were significant differences between the phorbol‑12‑myristate‑13‑acetate (TPA)‑induced A549 cells treated with Cal and the untreated cells in the rates of migration and invasion. The levels of MMP‑2, MMP‑9, E‑cad and integrin β1 in the TPA‑induced A549 cells changed markedly, compared with the untreated cells. In addition, the suppression of Cal was affected by the PKC inhibitor, AEB071, an ERK1/2 inhibitor, PD98059. The results of the present study indicated that Cal inhibited the proliferation, adhesion, migration and invasion of the TPA‑induced A549 cells. The Cal‑induced repression of PKC‑α/ERK1/2, increased the expression of E‑Cad and inhibited the expression levels of MMP‑2, MMP‑9 and integrin β1, which possibly demonstrates the mechanism underlying the biological anticancer effects of Cal.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 413
·期 2
·577-584
·
IF 3.637
Xie Qilong,Xia Ming,Sun Dekui,Cao Jiangping,Xiao Yong,Lin Mingui,Hou Bo,Jia Litao,Li Debao
For simultaneous analysis of four fat-soluble tocopherols (α-, β-, γ-, and δ-) in edible oils, an efficient and green method using deep eutectic solvent-based liquid-phase microextraction (DES-LPME) coupled with reversed-phase high-performance liquid chromatography (RP-HPLC) was developed. The DESs formed by different quaternary ammonium salts and ethanol were used as the extractants. Tetrabutylammonium chloride (TBAC)-ethanol DES at a molar ratio of 1:2 achieved the best extraction efficiency. Under the optimized conditions, the detection limits were in the range of 2.1–3.0 ng mL −1 . The intra-day and inter-day repeatability were in the ranges of 3.9–5.3% and 4.8–7.1%, respectively, and the recoveries for the real samples varied from 80.7% to 105.4%. The developed method was successfully employed for the determination of all four tocopherol homologues with an RP-HPLC system containing a COSMOSIL π-NAP column in five edible oils collected locally.
Graphical abstract
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JOURNAL OF MICROENCAPSULATION
·
2019
·
IF 2.04
Yingting Wang,Along Zuo,Xiaoyan Huang,Ying Ying,Xinsheng Shu,Xianxiong Chen,Yatao Yang,Junxian Ma,Guimiao Lin,Xiaomei Wang,Lin Mei,Gan Liu,Yingying Zhao
Taxane-based chemotherapy-loaded drug delivery systems have great potential for cancer treatment. The docetaxel (DTX)-loaded PAMAM-based poly (γ-benzyl-l-glutamate)-b-d-α-tocopheryl polyethylene glycol 1000 succinate (PAM-PBLG-b-TPGS) nanoparticles and the docetaxel (DTX)-loaded PAMAM-based poly (γ-benzyl-l-glutamate) (PAM-PBLG) nanoparticles were designed using a modified nanoprecipitation method. The particle size, encapsulation efficiency (EE), and in vitro release characteristics of the nanoparticles were tested. The effects of the two nanoparticles on the cellular uptake and cell viability on human cervical cancer cell line Hela and the human breast cancer cell line MCF-7 were compared. Furthermore, their antitumor efficiency was evaluated through in vivo tumour growth experiment in comparison with free DTX. PAM-PBLG-b-TPGS nanoparticles displayed high EE, smaller diameter, and a nice releasing profile. Besides, based on the high EE and ‘self-controlled’ drug release of the DTX-loaded PAM-PBLG-b-TPGS nanoparticles, they exhibited stronger cytotoxicity (lower survival rate) and higher uptake rate than DTX-loaded PAM-PBLG nanoparticles in Hela cells and MCF-7 cells. Furthermore, compared with DTX-loaded PAM-PBLG nanoparticles and free DTX, DTX-loaded PAM-PBLG-b-TPGS nanoparticles produced a potent anti-tumour effect. Thus, the DTX-loaded PAM-PBLG-b-TPGS nanoparticles provide a novel attractive nanocarrier for the DTX delivery of chemotherapy to human breast cancer cells and human cervical cancer cells.
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JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
·
2018
·
IF 0.715
Ti Wu,Shoukai Lin,Bisha Wu,Yu Chen,Suying Lin,Dahe Lin,Linxia Chen,Zhisheng Zhang,Jincheng Wu
Cold-stored fruits of the loquat (Eriobotrya japonica Lindl.) plant suffer from strong lignification, which leads to a hard texture and a crude mouthfeel of the pulp. However, the signalling mechanisms of loquat fruits under cold storage remain unclear. In this study, the heterotrimeric G protein α subunit (Gα) gene (EjLGA1) from loquat was isolated, and in silico analysis was performed. The levels of EjLGA1 expression in cold-stored loquat fruits with different pretreatments were also investigated. The largest open-reading frame of EjLGA1 is 1173 bp, encoding a polypeptide of 390 amino acids. The nucleotide and amino acid sequences of the Gα subunit are highly conserved among loquat and 48 other seed plants, indicating that the loquat Gα subunit likely has similar functions in other plants. A reliable model of the loquat Gα subunit was constructed, which revealed the structure of the subunit. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) results showed that EjLGA1 is highly expressed under cold storage, which suggests the significance of EjLGA1 function in loquat fruits in response to cold stress. However, ethephon and 1-methylcyclopropene (1-MCP) pretreatments did not regulate EjLGA1expression. These results provide new insight into the signalling mechanism in cold-stored loquat fruits.
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危险品化学品经营许可证（带存储）