TEXTILE RESEARCH JOURNAL
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2017
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IF 1.443
Liu Liu,Yeping Xiang,Ruiyun Zhang,Bei Li,Jianyong Yu
In this study, different dosages of NaClO were used in the 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO)-laccase degumming system to remove non-cellulosic materials, and their effects on the structure of hemp fibers were analyzed and discussed. A scanning electron microscope was used to depict the surface morphology of fibers after oxidative degumming under various dosages of NaClO in the TEMPO-laccase degumming system. Chemical composition analysis was used to determine the content changes of the different components. Meanwhile, the content of functional groups was also discussed. Fourier transfer infrared spectroscopy, nuclear magnetic resonance spectroscopy, and X-ray diffraction were employed to evaluate the microstructural changes of degummed hemp fibers obtained from the degumming processes with different dosages of NaClO. The results showed that after the TEMPO-laccase system degumming process with a NaClO dosage of 16%, the cleanest and smoothest surface of degummed fibers could be observed and the non-cellulosic materials were significantly removed without any crystalline transformation or damage in the cellulose. This research could shed light on determining favorable operation parameters for hemp oxidation degumming and increasing the degumming efficiency, as well as in the oxidation control and quality assurance of hemp fibers for textile downstream end uses.
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BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
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2016
·Vol. 64
·期 2
·251-258
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IF 1.429
Hong-De Yan,Zhao Wang,Jun-Qing Qian
A mycelium-bound lipase from Aspergillus oryzae (AOL) exhibited excellent enantioselectivity for kinetic resolution of ( RS )-1-phenylethanol (( RS )-1-PE) in organic solvent. The various reaction parameters affecting the conversion and enantioselectivity were studied, including type of acyl donor, solvent, molar ratio, temperature, enzyme amount, and substrate concentration. The optimum reaction conditions were found to be transesterification with vinyl acetate at 30 °C in methyl tert -butyl ether with a vinyl acetate: ( RS )-1-PE molar ratio of 1:1 and an enzyme concentration of 60 g/L. At the optimum reaction conditions, the conversion could reach above 46% with >99% enantiomeric excess of the product, ( R )-1-phenylethyl acetate, when the substrate concentration was below 1.4 M. The enzyme displayed an excellent enantioselectivity with an E -value of >200 and a strong tolerance for high substrate concentration of up to 1.8 M. Those results indicated that AOL was a promising biocatalyst in the kinetic resolution of ( RS )-1-PE.
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CHEMICAL ENGINEERING JOURNAL
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2016
·
IF 5.31
Feng Xu,Ying Yu,Jian Yan,Qibin Xia,Haihui Wang,Jing Li,Zhong Li
An ultrafast synthesis method is developed for the preparation of composites of graphite oxide and HKUST-1, GrO@HKUST-1. Fast synthesis of GrO@HKUST-1 composites can be quickly achieved at room temperature within 1 min. The synthesized composites were characterized by XRD, SEM, N 2 adsorption, FTIR and TGA. The isotherms of CO 2 and N 2 on the as-synthesized materials were measured and the isosteric heats of CO 2 adsorption were estimated. The CO 2 /N 2 adsorption selectivities were predicted by means of ideal adsorbed solution theory (IAST). Results show that the GrO@HKUST-1 composites have higher BET surface area and pore volume than the parent HKUST-1. The CO 2 adsorption capacity of 2GrO@HKUST-1 (2% of GrO) is up to 9.02 mmol/g at 1 bar and 273 K, giving an increase of 32% in comparison of the parent HKUST-1. The isosteric heat of CO 2 adsorption on 2GrO@HKUST-1 is slightly higher than that of HKUST-1, suggesting the stronger interaction between CO 2 molecules and 2GrO@HKUST-1. The CO 2 /N 2 adsorption selectivity of 2GrO@HKUST-1 is significantly enhanced over pristine HKUST-1. At 1 bar, the CO 2 /N 2 adsorption selectivity of 2GrO@HKUST-1 is up to 186, while that of parent HKUST-1 is only 103. This rapid room temperature synthesis route is promising for new MOF-based composites.
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Frontiers in Microbiology
·
2016
·
IF 4.165
Han Rui,Li Fangbai,Liu Tongxu,Li Xiaomin,Wu Yundang,Wang Ying,Chen Dandan
It is widely recognized that the outer membrane c-type cytochromes (OM c-Cyts) of metal-reducing bacteria play a key role in microbial metal reduction processes. However, the in situ redox status of OM c-Cyts during microbial metal reduction processes remain poorly understood. In this study, diffuse-transmission UV/Vis spectroscopy is used to investigate the in situ spectral reaction of Cr(VI) reduction by c-Cyts in intact Shewanella oneidensis MR-1 cells under different incubation conditions. The reduced c-Cyts decreased transiently at the beginning and then recovered gradually over time. The Cr(VI) reduction rates decreased with increasing initial Cr(VI) concentrations, and Cr(III) was identified as a reduced product. The presence of Cr(III) substantially inhibited Cr(VI) reduction and the recovery of reduced c-Cyts, indicating that Cr(III) might inhibit cell growth. Cr(VI) reduction rates increased with increasing cell density. The highest Cr(VI) reduction rate and fastest recovery of c-Cyts were obtained at pH 7.0 and 30∘C, with sodium lactate serving as an electron donor. The presence of O2 strongly inhibited Cr(VI) reduction, suggesting that O2 might compete with Cr(VI) as an electron acceptor in cells. This study provides a case of directly examining in vivo reaction properties of an outer-membrane enzyme during microbial metal reduction processes under non-invasive physiological conditions.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2017
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IF 2.714
Yun-Xia Wang,Kiran Gupta,Jian-Rong Li,Baoling Yuan,Jia-Cheng E. Yang,Ming-Lai Fu
A novel easily separated magnetic chalcogenide composite, KMS-1/L-Cystein/Fe 3 O 4 (KCF), was successfully synthesized using bifunctional linker (L-Cystein) to connect KMS-1 and Fe 3 O 4 nanoparticles, and was examined to remove ciprofloxacin (CIP) from aqueous solution. The composite was thoroughly characterized by XRD, SEM, EDX, FTIR and magnetization measurements techniques. The adsorption experiments were carried out and investigated under different conditions, such as contact time and pH of solution. The CIP removal reaction was found to better follow a pseudo-second-order kinetic model than the pseudo-first-order. The Langmuir adsorption isotherm was employed to understand the removal process. The pH of solution is critical for the adsorption of CIP on KCF, and the maximum adsorption (181.32 mg/g) of CIP could be reached under pH 6. In addition, the KCF adsorbent showed magnetization and operational stability, and it could be readily separated from solution by applying an external magnetic field. The high stability, performance, inexpensive, and easily recyclable KCF composite may serve as a promising adsorbent for water treatment.
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Environmental Science-Nano
·
2017
·Vol. 4
·期 12
·2395-2404
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IF 6.047
Hong-Wei Luo,Xin Zhang,Jie-Jie Chen,Han-Qing Yu,Guo-Ping Sheng
Although the existence of Fe(III) (hydr)oxide nanoparticles (NPs) in natural environments has been recently recognized, their migration and transformation, which are substantially affected by the microbial reduction process, remain unclear. In this study, hematite bioreduction and magnetite formation induced by Shewanella oneidensis MR-1, a typical metal-reducing bacterial strain, were investigated using an integrated approach that combined experiments and computational simulations at the molecular scale. High-resolution transmission electron microscopy coupled with selected area electron diffraction (SAED), Raman spectroscopy and magnetic property analyses confirmed the biogenic magnetite formation. The electron paramagnetic resonance (EPR) spectra show a decay of the EPR intensity with incubation time, implying that the biotransformation of hematite to magnetite led to concurrent changes in their contents and local structures. X-ray absorption fine spectroscopy (XAFS) identified a time-resolved structural evolution of Fe(II)/Fe(III) coordination. The octahedral configuration upon Fe(II) production was subsequently validated by DFT calculations. Microbial reductive dissolution provided various intermediate states and new chemical environments regarding Fe(II)/Fe(III) complexes, such as monodentate and bidentate coordination patterns. Bioreduction caused the breakage of iron–oxygen bonds in hematite and the concomitant formation of Fe complexing microstructures for biogenic magnetite production. All these findings reveal the underlying mechanisms for the biotransformation of Fe minerals at the molecular scale and may allow us to better understand the speciation, immobilization and bioavailability of hematite NPs in natural systems.
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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
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2024
·
IF 4.3
Xin Zhao,Chao Chen,Weina Han,Min Liang,Yuanyuan Cheng,Yingjie Chen,Defeng Pang,Haoqi Lei,Xuefei Feng,Shifeng Cao,Zhixiong Li,Jinhui Wang,Yan Zhang,Baofeng Yang
Lung cancer is a leading cause of cancer-related deaths worldwide. Recent studies have identified pyroptosis, a type of programmed cell death, as a critical process in the development and progression of lung cancer. In this study, we investigated the effect of EEBR, a new compound synthesized by our team, on pyroptosis in non-small cell lung cancer cells (NSCLC) and the underlying molecular mechanisms. Our results demonstrated that EEBR significantly reduced the proliferation and metastasis of NSCLC cells in vitro. Moreover, EEBR-induced pyroptosis in NSCLC cells, as evidenced by cell membrane rupture, the release of cytokines such as interleukin-18 and interleukin-1 beta and the promotion of Gasdermin D cleavage in a Caspase-1-dependent manner. Furthermore, EEBR promoted the nuclear translocation of NF-κB and upregulated the protein level of NLRP3. Subsequent studies revealed that EEBR-induced pyroptosis was suppressed by the inhibition of NF-κB. Finally, EEBR effectively suppressed the growth of lung cancer xenograft tumours by promoting NSCLC pyroptosis in animal models. Taken together, our findings suggest that EEBR induces Caspase-1-dependent pyroptosis through the NF-κB/NLRP3 signalling cascade in NSCLC, highlighting its potential as a candidate drug for NSCLC treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Yijun Tu,Yijie Cai,Jiachuan Lei,Jianqing Yu
The programmed death-ligand 1 (PD-L1) protein plays a key role in immune responses. Scutellarin (SCU), as a flavonoid, has a variety of bioactivities. In this study, the human PD-L1 was obtained by expression and purification, and the interaction mechanisms between PD-L1 and SCU were revealed through multi-spectroscopy and computer simulation. Fluorescence data indicated that the quenching of PD-L1 by SCU was mainly static quenching, and primarily driven by hydrogen bonding and van der Waals forces. The binding constant (K a ) was decreased from 2.05 ± 0.55 × 10 4 L·mol −1 to 0.28 ± 0.08 × 10 4 L·mol −1 with increasing temperature. Meanwhile, the changes in the microenvironment of PD-L1 were revealed by the synchronous and the 3D fluorescence data. In addition, the melting temperature of PD-L1 increased by 1.67 °C after binding with SCU. Moreover, the circular dichroism data showed that SCU changed the secondary structure of PD-L1 by increasing α-helix content and decreasing β-sheet content. Furthermore, the binding modes between SCU and PD-L1 and the key residues involved in the interaction were revealed by molecular docking and molecular dynamics. These findings supported SCU as an alternative ICB therapeutic strategy and provided evidence for computer-based drug design strategies.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Xin Li,Kun Xu,Yiyang Bi,Dongjie He,Xin Wang,Ke Li,Qun Liu,Yu Zhang
A new HKUST-1@covalently crosslinked agarose aerogel (HKUST-1@CCAGA) was synthesized for the highly effective elimination of Methylene Blue (MB). Firstly, the covalently crosslinked agarose aerogel (CCAGA) was obtained by hydrothermal crosslinking reaction with epichlorohydrin (ECH) as crosslinker, which remained stabilized under hydrothermal and solvothermal conditions. Then, HKUST-1 was made to bind to CCAGA by in situ solvothermal assays, and the HKUST-1 loading rate reached 47.4 % based on thermogravimetric data and calculated using the cross over method. Meanwhile, the SEM exhibited the complete 3D honeycomb structure of CCAGA, and HKUST-1 particles uniformly distributed on its layers. Additionally, the specific surface area of HKUST-1@CCAGA can reached 648.59 m 2 ·g −1 . The HKUST-1@CCAGA composite was utilized for MB removal, achieving a high adsorption capacity of 424.30 mg·g −1 at pH 8. The adsorption of MB was also maintained at 80 % after 5 cycles of the experiment. The composite aerogel exhibits good recyclability and has excellent adsorption capacity.
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ACS Applied Nano Materials
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2024
·
IF 5.3
Qinglin Chen,Long Tian,Ming Ruan,Yi Liu,Cheng Peng,Weigang Cui,Wei Liang,Shaoyun Shan,Tianding Hu
Fenton-like reactions with Fe-based metal–organic framework (MOF) catalysts have been extensively explored in the field of environmental remediation. However, easy precipitation of Fe2+/Fe3+ and the production of sludge under basic conditions caused catalyst loss and greatly limited their large-scale application in industry. The development of an Fe-free Fenton-like reaction is of extreme importance and remains in its infant stage. Herein, a series of Fe-free dual MOF nanoparticles (HKUST-1/ZIF-67-X) were fabricated by in situ coating of ZIF-67 on HKUST-1 and were systematically analyzed by various characterization techniques, such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR), X-ray photoelectron spectroscopy (XPS), and N2 adsorption–desorption isotherms. Subsequently, these materials were applied in catalyzing methylene blue (MB) degradation. The effects of several operation parameters, i.e., pH, H2O2 dosage, catalyst dosage, and reaction temperature, on MB degradation were investigated. It was unveiled that HKUST-1/ZIF-67-7% exhibited an outstanding catalytic activity without the production of any sludge, which could reach as high as 93.29% MB degradation efficiency within 40 min. This was attributed to the unique core–satellite histoarchitecture of HKUST-1/ZIF-67-7% and the synergistic effect between HKUST-1 and ZIF-67. The HKUST-1/ZIF-67-7% composite still achieved up to 80.17% MB degradation efficiency at the fifth catalysis cycle. Importantly, HKUST-1/ZIF-67-7% exhibited significant catalytic efficiency under a wide pH range (4.2–10.1) and top catalytic efficiency at the near neutral pH value. The low cost, environment benignancy, satisfactory degradation efficiency, wide pH application range, and excellent reusability emphasize its great application potential in Fenton-like degradation of pollutants. This contribution could provide a paradigm investigation for designing non-iron-based MOF catalysts to solve the increasingly pressing pollution issues.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2024
·
IF 5.8
Xiaohui Zhang,Xinyue Zhong,Xiaohua Zhang,Yu Liu,Shuai Ma,Chenglong Yu,Zhenxing Yue
Dielectric ceramics in the microwave band are one of the crucial materials for mobile communication technology, and the studying of their phase evolution and defect behavior can help to understand and reduce dielectric loss. In this work, (1- x )BaNd 1.76 Bi 0.24 Ti 5 O 14 - x Ba 0.6 Sr 0.4 La 4 Ti 4 O 15 ( x = 0.2, 0.4, 0.6 and 0.8, (1- x )BNT- x BSLT) ceramics were synthesized by solid-state reaction method. X-ray diffraction (XRD) and Raman spectra analysis reveal that tungsten bronze and hexagonal perovskite phases coexist in the ceramics, and BaNd 2 Ti 3 O 10 is indexed as the main crystalline phase at x = 0.4, 0.6, leading to a deterioration of the microwave dielectric properties. The defect-related relaxation behavior and its effect on microwave dielectric properties were analyzed by dielectric temperature spectrum and thermally stimulated depolarization current (TSDC). The relationship between the dielectric constant and the electric field distribution was solved using the eigenmode of the high frequency structure simulator. In short, the (1- x )BNT- x BSLT ceramics at x = 0.8 exhibit excellent microwave dielectric properties: ε r = 47.2, Q × f = 16,800 GHz, and τ f = −4.6 ppm/°C. The microwave dielectric response of BNT-BSLT composite ceramics is closely related to the phase composition and defects, which is informative for the development of low dielectric loss ceramics.
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TALANTA
·
2024
·
IF 5.6
Rui Liu,Chaojun Zhang,Tianheng Wu,Rijia Liu,Yuan Sun,Jing Ma
In this work, a novel HKUST-1/CoFe 2 O 4 /g-C 3 N 4 electrode was successfully prepared via the hydrothermal method and the high-temperature calcination method, which can be applied as an electrochemical sensor for the precise detection of ciprofloxacin (CIP) in physiological samples. The novel electrode was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FT-IR), and its electrochemical performance was further evaluated via the cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The results demonstrated that the HKUST-1/CoFe 2 O 4 /g-C 3 N 4 electrode exhibited an optimal linear range of 0.05–180 μmol L −1 for the CIP detection, which demonstrated a low limit of detection (LOD) of 0.0026 μmol L −1 and a low limit of quantitation (LOQ) of 0.0087 μmol L −1 , respectively. Additionally, the novel semiconductor sensors exhibited exceptional selectivity, stability and repeatability in the determination of CIP. The recovery rate of CIP was found to range from 98.00% to 104.00% in serum, with the relative standard deviations (RSD) below 2.62% (n = 5), while the recovery rate of CIP was found to range from 96.00% to 105.00%, with the RSD less than 3.23% (n = 5) in urine. The current study extends to the application of the semiconductor-based electrochemical sensors and offers a new approach for the clinical pharmaceutical analysis to ensure medication safety, which could provide valuable insights into the potential of semiconductor sensors for future clinical applications.
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