Plant Communications
·
2025
·Vol. 6
·期 10
·
IF 11.6
Qing-Song Yuan,Lu Luo,Haixia Shi,Hui Wang,Jiuchun An,Yanping Gao,Jiao Xu,Xiaohong Ou,Ye Yang,Karim M. Tabl,Lanping Guo,Luqi Huang,Tao Zhou
Nitrogen and auxin uptake play pivotal roles in seed germination and development. Gastrodia elata , a fully mycoheterotrophic plant, depends entirely on its symbiotic association with Mycena for early growth and seed germination. The process by which Mycena supplies nitrogen nutrients and auxin, which are deficient in G. elata , remains poorly understood. In this study, genome-scale analysis of G. elata revealed the loss of genes associated with nitrogen utilization and indole-3-acetic acid (IAA) biosynthesis, which are retained in Mycena . Further analysis of the dynamic transcriptomic interactions between G. elata seeds and Mycena across different symbiotic stages revealed that genes involved in nitrogen- and tryptophan-dependent IAA biosynthesis were significantly upregulated in Mycena during the symbiotic germination of G. elata seeds . Concurrently, G. elata seeds exhibited increased expression of genes involved in the hormone signal transduction pathway and the starch and sucrose metabolism pathway. Functional disruption of nitrite reductase (MyNir, EVM0012344) and amidase (MyAmid, EVM0010270), key enzymes in nitrogen assimilation and IAA biosynthesis in Mycena , significantly impaired the symbiotic germination of G. elata seeds. This disruption interfered with energy supply, caused cellular restructuring, and altered hormonal signaling crosstalk. In conclusion, our findings provide novel insights into the mutualistic symbiotic relationship between Mycena and G. elata . Specifically, the fungus Mycena compensates for the incomplete nitrogen metabolism of its plant partner, G. elata , and supplies IAA, thereby promoting seed germination. These results shed light on plant-fungal symbiotic associations from the perspective of nitrogen utilization.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-11
·
IF 3.9
Chen Yuan-Bin,Xiong Siyi
The Na 5 Tm 1 − x (Y 1/3 Yb 2/3 ) x (MoO 4 ) 4 ceramics (x=0-0.18) were successfully synthesized via the solid-state reaction method. XRD analysis revealed that they possessed a single-phase tetragonal scheelite structure belonging to the space group I4₁/a(88). SEM analysis demonstrated that the samples sintered at 600 °C exhibited a dense microstructure, with a relative density as high as 94.9% and an average grain size ranging from 2.172 μm to 2.424 μm. The relative density serves as an external factor influencing ε r and Q×f, while τ f is related to bond valence. The ionicity of the Tm-O bond, and the lattice energy and bond energy of the Mo-O bond are the intrinsic factors determining ε r , Q×f, and τ f , respectively. Raman spectroscopy provided an intuitive characterization of the vibrational properties of the [MoO 4 ] 2− tetrahedra within the ceramics. Although the pure phase Na₅Tm(MoO 4 ) 4 ceramic exhibited optimal dielectric properties (ε r =8.025, Q×f=18,967 GHz, and τ f = -101 ppm/°C) at 625 °C, the Na 5 Tm 0.91 Y 0.03 Yb 0.06 (MoO 4 ) 4 ceramic sintered at 600 °C demonstrated superior dielectric properties (ε r =7.82, Q×f=34,752 GHz, and τ f = -71.9 ppm/°C).
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JOURNAL OF MOLECULAR MEDICINE-JMM
·
2024
·
IF 4.8
Wang Pengtao,Tao Wei,Li Qiujie,Ma Wanting,Jia Wei,Kang Yuting
Indole-3-aldehyde (I3A) is an intestinal microbial metabolite that regulates inflammation in various inflammatory diseases; however, its role in chronic obstructive pulmonary disease (COPD) remains unclear. This study aimed to investigate the anti-inflammatory effects and molecular mechanisms of I3A in COPD. We constructed in vivo models using cigarette smoke (CS)-stimulated mice and in vitro models using cigarette smoke extract (CSE)-stimulated MH-S cells. The results demonstrated that I3A significantly alleviated bronchial obstruction in mice with COPD and reduced the expression of inflammatory factors such as TNF-α, IL-1β, and IL-6. Additionally, I3A decreased the levels of matrix metalloproteinases MMP2, MMP12, and inhibited the NF-κB p65/NLRP3 pathways. Further investigation revealed that I3A inhibited NF-κB activity by suppressing p65 phosphorylation and nuclear translocation in CSE-stimulated MH-S cells. The activation of the NF-κB and NLRP3 signaling pathways is mediated by histone deacetylase 5 (HDAC5) and HDAC6, both of which are inhibited by I3A. Subsequent experiments indicated that aryl hydrocarbon receptor (AHR) knockdown attenuated the inhibitory effect of I3A on pro-inflammatory cytokines and the HDACs/NF-κB/NLRP3 signaling pathways, highlighting the dependence of I3A's anti-inflammatory effects on the AHR receptor. Key messages
I3A effectively reduced lung inflammation in COPD mice by inhibiting the NF-κB pathway.
In CSE-stimulated MH-S cells, I3A suppressed p65 phosphorylation and nuclear translocation, thereby inhibiting NF-κB activity.
The activation of the NF-κB/NLRP3 pathways by HDAC5 and HDAC6 was diminished by I3A.
Through the activation of the AHR receptor, I3A suppressed the activities of HDAC5/6, leading to a decrease in inflammatory factor levels.
Graphical abstract
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Food Bioscience
·
2025
·
IF 5.9
Yuling Tang,Xunjiao Wei,Yonghua Wang,Wen Chen
Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are principal omega-3 polyunsaturated fatty acids (PUFAs) nutritional and pharmaceutical interest. However, their near-isomeric structures render large-scale separation technically demanding and costly. Here we evaluate human fatty-acid-binding protein 7 (FABP7) as a biorecognition element capable of discriminating between these two lipids. Recombinant FABP7, expressed and purified from Escherichia coli , was analyzed for its binding affinity toward DHA and EPA by bio-layer interferometry (BLI). FABP7 bound DHA with an equilibrium dissociation constant K d = 80.18 ± 3.71 mmol/m 3 , whereas binding to EPA was 7.5-fold weaker (K d = 600.2 ± 30.2 mmol/m 3 ). Competitive BLI experiments confirmed that DHA efficiently displaced pre-bound EPA, while EPA was largely unable to remove DHA even at millimolar excess. These findings demonstrate that FABP7 possesses intrinsic molecular selectivity for DHA over EPA and could therefore serve as the affinity component of bio-based fractionation platforms. This work establishes a quantitative framework for engineering FABP-derived resins or sensors aimed at sustainable, low-energy purification of high-value ω-3 lipids.
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Probiotics and Antimicrobial Proteins
·
2025
·
IF 4.4
Zhang Shuo,Gao Peichao,Wang Qunxin,Liu Shuhui,Okoth Sheila,Zhang Meihua,Ji Helong,Yan Liping,Fan Wentao,Song Suquan
Fatty liver hemorrhagic syndrome (FLHS) has become a common and serious disease in intensive laying hen farming, seriously endangering the productivity and health of laying hens. Although probiotics have been recognized as an emerging candidate therapeutic strategy for liver disease, their effective substances and mechanisms for alleviating FLHS remain to be elucidated. In this study, corticosterone (Cort) was applied to induce FLHS in laying hens, and Lactobacillus plantarum ( L. plantarum 1–2-3) was used for the intervention. The results showed that L. plantarum 1–2-3 treatment significantly ameliorated Cort-induced hepatic steatosis and liver damage in laying hens, as evidenced by decreased levels of triglyceride (TG), total cholesterol (TC), aspartate aminotransferase, and alanine aminotransferase. Importantly, we found that L. plantarum 1–2-3 could biosynthesize gamma-aminobutyric acid (GABA) by expressing glutamic acid decarboxylase. Surprisingly, feeding GABA to laying hens replicated the effect of L. plantarum 1–2-3 in alleviating Cort-induced FLHS. GABA significantly down-regulated the mRNA expression of Acc , Fasn , Cd36 , and Gpam while upregulating the levels of Ppara and Cpt1a . An in vitro verification study showed that GABA decreased TG and TC content in Leghorn male hepatoma cells. Moreover, GABA significantly enhanced antioxidant enzyme activity, inhibited oxidative stress in hepatocytes, regulated the AMPK/NLRP3 signaling pathway, and reduced the protein expression of NF-κB, TNFα, and IL-1β. In conclusion, our study demonstrated that L. plantarum 1–2-3 could effectively alleviate FLHS in laying hens, while GABA was an important postbiotic for L. plantarum 1–2-3 to exert lipid-lowering and anti-inflammatory effects.
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ACS Omega
·
2025
·
IF 4.3
Du Duc Nguyen,Dat Van Nguyen,Hue Van Nguyen,Giang Huong Thi Vu,Ha Xuan Nguyen,Hai Hong Thi Le,Dien Huu Pham,Trang Ha Thi Nguyen,Tai Minh Trinh,Nga Thuy Nguyen,Hue Minh Thi Nguyen,Hoan Quoc Duong
A novel series of benzothiazole derivatives was synthesized using straightforward and easily implementable procedures, achieving a high yield. Among these synthesized compounds, amino acids containing the benzothiazole moiety were successfully produced through an 8-step process, with yields reaching as high as 95%. Notably, a serendipitous compound containing both benzothiazole and benzo[1,4]oxazin-3(4H)-one moieties was also synthesized using the same protocol, bypassing purification at step 7 and proceeding directly to hydrolysis. This highlights the unique role of the coupling reagent HATU (hexafluorophosphate azabenzotriazole tetramethyluronium) in the reaction, as it facilitated high yields, reaching up to 90%. The structures of the newly synthesized compounds were confirmed through spectral analysis. Density functional theory calculations suggested that energy barriers can be overcome by utilizing the energy from an exothermic reaction, enabling the thermodynamically favorable formation of this novel structure. Compounds 6d and 6f demonstrated significant inhibitory activity against the enzyme acetylcholinesterase, with IC50 values of 32.00 and 25.33 μg/mL, respectively. Molecular docking and molecular dynamics analyses indicate that compounds 6d and 6f hold potential for combating Alzheimer’s disease, due to their interactions with critical amino acid residues and structural stability.
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JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
·
2022
·Vol. 36
·期 8
·e23084
·
IF 3.568
Qian Shang,Li Zhu,Weina Shang,Jia Zeng,Yong Qi
Dioscin is a natural product that possesses protective effects on multiple chronic injuries, but its effects on asthma are not fully understood. Herein, we evaluated its effects on asthmatic mice established by ovalbumin (OVA) sensitization and challenges and further explored the mechanism. Inflammatory cells in bronchoalveolar lavage fluids (BALFs) were analyzed using Diff-Quik staining. OVA-specific immunoglobulin E (IgE)/IgG1 in serum and inflammatory cytokines (interleukin 4[IL-4], IL-5, IL-13, and tumor necrosis factor-α) in BALFs and lung tissues were measured using Enzyme-Linked Immunosorbent Assay Kits. Hematoxylin and eosin, periodic acid–Schiff, and immunohistochemistry staining showed histopathological changes in lung tissues. Epithelial–mesenchymal transition (EMT) in human bronchial epithelial (16HBE) cells was assessed by immunofluorescence staining. Hydroxyproline content was used to evaluate collagen deposition. Polymerase chain reaction and Western blot were performed to measure messenger RNA and protein expression. We found that dioscin treatment (particularly at the dose of 80 mg/kg) significantly inhibited pulmonary inflammation in asthmatic mice, as evidenced by the decreased serum OVA-specific IgE/IgG1 and the reduced inflammatory cells and cytokines in BALFs and lung tissues. Moreover, dioscin effectively ameliorated the goblet cell hyperplasia, mucus hypersecretion, collagen deposition, and smooth muscle hyperplasia in the airways of asthmatic mice. Mechanistically, dioscin restrained the activated TGF-β1/Smad2/3 and protein kinase B (AKT) signal pathways in lung tissues and potently reversed the TGF-β1-induced EMT and phosphorylation of Smad2/3 and AKT in 16HBE cells. Collectively, dioscin displayed protective effects on OVA-induced asthmatic mice via adjusting TGF-β1/Smad2/3 and AKT signal pathways, supporting the fact that dioscin could be a candidate for chronic asthma prevention in the future.
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BIOMATERIALS
·
2021
·
IF 12.479
Jiankun Guan,Yuxin Wu,Xin Liu,Huimin Wang,Ningbing Ye,Zheng Li,Chen Xiao,Zhijie Zhang,Zifu Li,Xiangliang Yang
Cancer stem cells (CSCs) present grand challenges for triple-negative breast cancer (TNBC). Conventional chemotherapy drugs, including Camptothecin (CPT), not only cannot eradicate CSCs but also foster a suppressive immune microenvironment for the initiation and proliferation of CSCs. Herein, we report a novel prodrug CPT–SS–NLG919 (CN) and its nanoformulation [email protected] ( [email protected] ), which potently suppress CSCs by regulating CSCs niche in murine TNBC 4T1 tumors. Via inducing immunogenic cell death (ICD) and simultaneous inhibiting indoleamine 2, 3-dioxygenase (IDO), CN and [email protected] promote DC maturation, decrease Treg cells, mitigate tryptophan consumption, and reduce the amount of IL-6, IL-13, and TGF-β, converting CSCs niche to a hostile condition for CSCs to live in and eliminating CSCs efficiently, thereby inducing efficient tumor inhibition in 4T1 tumor models. Our work represents a new paradigm of eliminating CSCs by regulating tumor immune microenvironment and suggests that CN and its nanoformulation [email protected] are promising candidates for the treatment of intractable TNBC.
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CNS Neuroscience & Therapeutics
·
2025
·Vol. 31
·期 12
·e70682
·
IF 5
Haitao Zhang,Ning Zhang,Xiaotong Yang,Jiejie Zhang,Xiaoli Ge,Lei Wang,Shan Wang,Ya Wen
This study provides a novel insight into the molecular mechanism whereby NBP protects mitochondria against ischemia/hypoxia damage, offering a promising drug for mitochondria‐targeting therapeutics for ischemic stroke.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Tingxuan Yan,Mengting Gao,Wei Yuan,Jiahao Su,Yuhang Zhang,Jiaqian Gao,Konglin Wu
Sensitive and rapid detection of biomarkers remains a critical challenging task in disease monitoring, diagnosis, and therapeutic intervention. In this study, we developed a nanoflower-structured fluorescent sensor (ZBM) synthesized from Zn 2+ ions coordinated with dual ligands, 2-methylimidazole and 2-aminoterephthalic acid. Given that a lot of diseases such as malignant tumors are often associated with inflammatory responses, the key biomarker 3-nitrotyrosine (3-NT) which would be over-expressed was selected as the target analyte. The RGB values of colorimetric cards could be picked and embedded into a smartphone applet. The ZBM sensor exhibited exceptional responsiveness to 3-NT, demonstrating ultra-fast detection (<10 s), high quenching efficiency, strong anti-interference capability, and a low detection limit (0.59 μM). Theoretical calculations revealed that the selective fluorescence quenching mechanism of ZBM by 3-NT arises from the synergistic effects of photoinduced electron transfer (PET) and inner filter effect (IFE). Furthermore, the practical applicability of this sensor was validated through the determination of 3-NT in real biological samples, achieving satisfactory recoveries ranging from 97.86 % to 105.20 %.
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RARE METALS
·
2025
·
IF 11
Xiaolei Wu,Ren Huang,Yibing Zhang,Peng Lv,Suhua Chen,Ying Bai
en The polyanionic compound Na 3 V 2 (PO 4 ) 3 (NVP) has emerged as a competitive cathode material for sodium-ion batteries (SIBs), owing to its high-voltage redox activity (3.4 V vs. the Na + /Na reference couple) and NASICON-type framework that enables superior ionic conductivity. However, the electrochemical performance of NVP is hampered by low intrinsic electronic conductivity, particularly under ultra-fast charging and long-term cycling conditions. Herein, a hierarchical micro-nano flower-like Na 3 V 2 (PO 4 ) 3 with a carbon-coating layer (HF-NVP@C) is synthesized by a facile n-propanol-assisted precipitation method. Theoretical analysis and experimental tests reveal that the polar-driven hydrogen bond engineering, manifested in the distinct molecular interaction between n-propanol and ultrapure water (characterized by a shorter O-H bond length of 1.859 Å and higher binding energy of −0.312 eV compared to methanol and ethanol counterparts), underpins the formation of the unique hierarchical micro-nano flower-like architecture. This hierarchical architecture creates an expanded interfacial area, enhances ionic transport pathways, and increases electrochemically active sites. Furthermore, a glucose-derived carbon coating (∼2 nm in thickness) uniformly adheres to the NVP surface, effectively enhancing its electronic conductivity. Thus, the constructed HF-NVP@C exhibits superior rate capability (68.1 mAh g −1 at 150C) and long-term cycling stability (58.7% capacity retention after 5000 cycles at 1C). This work proposes a viable strategy for designing high-performance polyanionic cathodes using hydrogen bond engineering. 摘要 zh 聚阴离子化合物Na 3 V 2 (PO 4 ) 3 @C(NVP)因其具有高电压氧化还原活性(相对于Na + /Na参比电对为3.4 V)以及能实现优异离子电导率的NASICON型骨架结构,已成为一种具有竞争力的钠离子电池(SIBs)正极材料。然而,由于NVP的本征电子电导率较低,导致其在超快充电和长期循环条件下的电化学性能较差。在本文中,通过一种简便的正丙醇辅助沉淀法合成了具有碳包覆层的分级微-纳花状Na 3 V 2 (PO 4 ) 3 @C(HF-NVP@C)。理论分析和实验测试表明,极性驱动的氢键工程体现在正丙醇与超纯水之间独特的分子相互作用上(其O-H键长为1.859 Å,比甲醇和乙醇对应的键长更短,结合能更高-0.312 eV),是形成这种独特分级微-纳花状结构的基础。这种分级结构扩大了活性材料的面积,增强其离子传输通道,并增加电化学活性位点。此外,葡萄糖衍生的碳涂层(约2 nm厚)均匀地附着在NVP表面,有效提高其电子电导率。因此,构建的HF-NVP@C展现出优异的倍率性能(150C下为68.1 mAh g -1 )和长期循环稳定性(1C下循环5000次后容量保持率为58.7%)。这项工作证明氢键工程设计高性能聚阴离子正极材料策略的可行,同时也为开发高性能钠离子电池正极材料提供了研究基础。
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INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
·
2019
·Vol. 43
·期 6
·2440-2450
·
IF 2.928
Chunmei Gu,Shumei Liu,Hongyue Wang,Haichuan Dou
Inflammatory response serves an important role in diabetic nephropathy (DN); however, the mechanism of inflammatory response results in renal damage is not yet clear. The aim of the present study was to investigate the role of the thioredoxin interacting protein (TXNIP)/NOD‑like receptor protein 3 (NLRP3) axis‑mediated activation of NLRP3 inflammasome in DN. A diabetic rat model was induced by streptozotocin injection. Hematoxylin and eosin (H&E) staining and streptavidin‑peroxidase staining were then used to examine the kidney tissue morphology, and TXNIP and NLRP3 protein expression levels, respectively. Furthermore, RNA interference technology was applied to silence the TXNIP gene. TXNIP and NLRP3 inflammasome activation‑associated proteins and mRNAs were detected by western blot analysis and RT‑qPCR, respectively. Enzyme‑linked immunosorbent assay was also used to examine interleukin (IL)‑1 and IL‑18 expression, while flow cytometry was performed to detect reactive oxygen species production. The data revealed that TXNIP and NLRP3 were overexpressed in kidney tissue of DN rats, and the level of antioxidant genes was downregulated. It was also observed that glucose promoted TXNIP expression and activation of NLRP3 inflammasome in a time‑dependent and dose‑dependent manner, therefore promoting inflammatory responses. Silencing of TXNIP gene resulted in the downregulation of NLRP3 inflammasome activation, and inhibited the expression levels of IL‑1 and IL‑18 in a high‑glucose environment. Furthermore, low expression of TXNIP promoted the levels of antioxidant genes and reduced the ROS levels. Taken together, the high‑glucose environment was able to upregulated the level of inflammatory factors by promoting the expression of TXNIP and the activation of NLRP3 inflammasome, consequently participating in DN.
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