ANALYST
·
2015
·Vol. 140
·期 5
·1672-1677
·
IF 4.107
Wei Wang,Na Zhao,Xiaoxiao Li,Jun Wan,Xiliang Luo
A novel controlled-release biosensor for isothermal amplified detection of ATP using Au nanocages (AuNCs) capped with a DNA molecular gate is reported for the first time, and has been successfully tested in intracellular ATP detection. Two kinds of SH-modified short strand DNAs S1 and S2 were assembled on the surface of the AuNCs by means of Au–thiolate bonding. The hybridization of a long-strand DNA S3 with the two immobilized SH-DNAs leads to the formation of molecular gates. The molecular gates were designed to inhibit the release of the fluorescent molecules such as Rhodamine-B (RhB), which were filled in the hollow interiors of AuNCs. The primer S4 was employed to play the role of a recognition moiety. The specificity recognition reaction between ATP and ATP aptamer gave rise to the primer S4 released from a double-stranded hybrid formed with the ATP aptamer. The released S4 will initiate the autonomous replication–scission–displacement process with the assistance of DNA polymerase and nicking endonuclease. As a result, the DNA synthesis and the DNA cycle achieved the opening of the DNA-based molecular gates and the significant amplification of the release of the guest molecules from AuNCs. In order to realize the cyclic enzymatic amplification of the release of the guest molecules from AuNCs, the long-strand S3 is ingeniously designed in such a way that it contains a Nb.Bpu10I nicking endonuclease recognition sequence and a sequence complementary to the primer S4. The fabricated system was demonstrated to be an efficient biosensor for target molecule detection qualitatively and quantitatively.
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高影响因子
RSC Advances
·
2019
·Vol. 9
·期 61
·35887-35894
·
IF 3.049
Ting Bian,Biao Sun,Sai Luo,Long Huang,Shan Su,Chunfeng Meng,Shichuan Su,Aihua Yuan,Hui Zhang
Pt-based nanocrystals with a twinned structure are highly desirable to achieve high performances in both catalytic activity and durability for methanol oxidation reaction (MOR). However, it still remains great challenge for producing such twinned nanocrystals due to the high internal strain energy of Pt. Here we present a seed-mediated approach to generate Au@PtCu nanostars with a five-fold twinned structure using Au decahedra as seeds. The composition of Pt/Cu in the nanocrystals was tuned by varying the molar ratio of Pt to Cu salt precursors with the amount of Au seeds being the same. Through composition optimization, Au@Pt1.2Cu nanostars achieved the highest specific (1.06 mA cm−2) and mass (0.18 mA mgPt−1) activities for MOR, which were about 5.9 and 1.6 times higher than those of commercial Pt/C, respectively. After accelerated stability test (ADT) for 1500 cycles, such nanostars remained ∼95% of specific activity compared to a loss of ∼28% for commercial Pt/C, indicting their superior durability for MOR. We believed that this enhancement may arise from the unique twinned structure and possible synergetic effect between Pt and Cu components.
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高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 23
·2960-2968
·
IF 2.378
Le Peng,Jun Zhou,Zhaoheng Liang,Yaping Zhang,Lucia Petti,Tao Jiang,Chenjie Gu,Danting Yang,Pasqule Mormile
Based on surface-enhanced Raman scattering (SERS) technology, Au@Ag@4MBA@5′-NH2-ssDNA probes and a Ag/TiO2@3′-NH2-ssDNA substrate were prepared and constructed into a sandwich structure to develop a high sensitivity bioassay of miRNA-21. The Au@Ag@4MBA@5′-NH2-ssDNA probes were prepared by immobilizing 5′-NH2-ssDNA onto the surfaces of 4MBA-labelled Au@Ag core–shell nanoparticles, and the Ag/TiO2@3′-NH2-ssDNA substrate was prepared by immobilizing 3′-NH2-ssDNA on the surface of Ag/TiO2 nanowires SERS-active substrates. The experimental results showed that the SERS-based sandwich bioassay of miRNA-21 presented a low limit of detection of 0.75 fM and a broad dynamic range from 1.0 fM to 1.0 nM. Also, the test data for the SERS-based sandwich bioassay were not only consistent with that of the real-time fluorescence quantitative polynucleotide chain reaction (RT-qPCR) method but also displayed higher detection sensitivity. It was shown that the SERS-based sandwich bioassay of miRNA-21 has importance for use in potential applications involving diagnosing clinical cancer patients.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Shenguang Ge,Yan Zhang,Lina Zhang,Linlin Liang,Haiyun Liu,Mei Yan,Jiadong Huang,Jinghua Yu
A trimetallic dendritic Au@PdPt nanparticles (Au@PdPt NPs) with intrinsic peroxidase-like catalytic activity were fabricated by a facile method. Au@PdPt NPs catalyzed the reduction of H 2 O 2 with the help of the electron mediator (thionine), which produced the electrochemical signals dramatically amplified. A sandwiched sensor was designed and implemented in the paper chip device for the determination of K-562 cells. The surface of dendritic Au@PtPd NPs was linked with folic acid by click chemistry. Folic acid was attached to the folate receptor (FR) of cell surface with high affinity. So, K-562 cells could be assayed based on folic acid-functionalized Au@PtPd NPs. On the other hand, folic acid-functionalized Au-paper working electrode on origami electrochemical device was fabricated, which could be used to capture K-562 cells. The twice utilization of folic acid for the capture of K-562 cells as well as the attachment of dendritic Au@PtPd NPs nanoprobe, which enhanced the selectivity of sensor for the determination of cells. The proposed biosensor exhibited excellent analytical performance toward the cytosensing of K562 cells with a wide detection linear range from 1.0 × 10 2 to 2.0 × 10 7 cells mL −1 and a detection limit of 31 cells mL −1 . Further, the proposed electrochemical sensor was applied to monitor cells. Au@PtPd NPs nonenzymatic catalysts could be used as signal-amplifying nanoprobes for ultrasensitive electrochemical sensing.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Min Zhang,Baolong Li,Yu Du,Gaojian Zhou,Yanyan Tang,Yanfen Shi,BiYu Zhang,Zhou Xu,Qingli Huang
The preparation and construction of novel nanostructures as targeting theranostic is of great significance for diagnosis and treatment of tumor. Herein, a novel PANI/ [email protected] @MnO 2 (PPAuMns) yolk-shell enzymatic theranostic agent was fabricated firstly via in situ deposition of Au nanoparticles (Au NPs) and reduction of KMnO 4 on the surface of PANI/PPy nanocomposites. In addition to high biocompatible and biodegraded performance, PPAuMns possessed amazing tumor microenvironment (TME)-responsive O 2 generation capacity, magnetic resonance imaging (MRI) property, enzymatic activity and excellent photothermal conversion effect. Chemotherapeutics doxorubicin and folic acid (FA) were further loaded and decorated on it for targeted tumor therapy. Experiments in vitro and in vivo indicated that DOX-loaded [email protected] demonstrated excellent synergistic anti-tumor effect combining chemotherapy (CT), chemo-dynamic therapy (CDT) and photothermal therapy (PTT). This work offers a paradigm to design and construct tumor diagnosis and therapy platforms employing TME-responsive synergistic strategy of photothermal ablation, chemo-dynamic and chemotherapy.
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高影响因子
Nanomaterials
·
2018
·Vol. 8
·期 5
·353
·
IF 3.504
Yue Chen,Yuanyuan Zhang,Qiangwei Kou,Yang Liu,Donglai Han,Dandan Wang,Yantao Sun,Yongjun Zhang,Yaxin Wang,Ziyang Lu,Lei Chen,Jinghai Yang,Scott Guozhong Xing
In this work, we report the enhanced catalytic reduction of 4-nitrophenol driven by Fe3O4-Au magnetic nanocomposite interface engineering. A facile solvothermal method is employed for Fe3O4hollow microspheres and Fe3O4-Au magnetic nanocomposite synthesis via a seed deposition process. Complementary structural, chemical composition and valence state studies validate that the as-obtained samples are formed in a pure magnetite phase. A series of characterizations including conventional scanning/transmission electron microscopy (SEM/TEM), Mössbauer spectroscopy, magnetic testing and elemental mapping is conducted to unveil the structural and physical characteristics of the developed Fe3O4-Au magnetic nanocomposites. By adjusting the quantity of Au seeds coating on the polyethyleneimine-dithiocarbamates (PEI-DTC)-modified surfaces of Fe3O4hollow microspheres, the correlation between the amount of Au seeds and the catalytic ability of Fe3O4-Au magnetic nanocomposites for 4-nitrophenol (4-NP) is investigated systematically. Importantly, bearing remarkable recyclable features, our developed Fe3O4-Au magnetic nanocomposites can be readily separated with a magnet. Such Fe3O4-Au magnetic nanocomposites shine the light on highly efficient catalysts for 4-NP reduction at the mass production level.Keywords:Fe3O4hollow microspheres;Fe3O4-Au magnetic nanocomposites;catalytic reduction;4-nitrophenol
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Cuiping Wang,Huan Zhang,Chunrong Wang
In this study, we developed an innovative approach that combines solid phase microextraction (SPME) with surface-enhanced Raman spectroscopy (SERS) to detect pesticide ferbam rapidly. An Au nano-glass capillary was fabricated by coating template-freely synthesized three-dimensional network Au nanostructures (3D-NW AuNSs) on the roughened surface of glass capillary and used for SPME and SERS respectively. Significant Raman signals were obtained by the SPME-SERS method, followed by detection of ferbam in water and orange juice samples with only 1 min SPME process. Results showed that Au nano-glass capillaries could achieve the detection of ferbam with limit of detection of 0.05 μg/L, and coefficient of determination ( R 2 ) of 0.9913. The recovery of predicted results was in the range of 88.46–103.29% and the relative standard deviation (RSD) was 3.5–8.2%. This study demonstrated potential capability of the SPME-SERS method for rapid (within 1 min) and sensitive detection of organic compounds in complex matrices. The SERS-active Au nano-glass capillary is easy to carry and operate, and is expected to play a role in the detection of trace pollutants.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Ruxi Lin,Rui Tan,Tao Yang,Qun Zhou,Juan Mu,Yan Zhao,Guoqi Zhang,Xiaojun Luo
Xanthine (XOE) is an important indicator for evaluating the freshness of aquatic products. This study successfully synthesized three-dimensional cobalt sulfide nanoflowers loaded with gold nanoparticles (CoS@Au). This material innovatively constructs an enzyme nanoflower cascade sensing system and integrates peroxidase like catalytic activity with surface enhanced Raman spectroscopy (SERS) enhancement capability on the same platform. In the presence of hydrogen peroxid (H 2 O 2 ), CoS@Au It can catalyze the oxidation of 3,3′, 5,5′-tetramethylbenzidine (TMB) to generate oxTMB, while producing significant color changes, strong UV–vis absorption, and Raman signals. This study presents a dual-mode sensor that effectively integrates the intuitive simplicity of colorimetry with the high sensitivity of SERS. This design overcomes the limitations of single-mode methods, such as restricted detection sensitivity and susceptibility to interference, by enabling cross-validation between the two modes. The constructed SERS/colorimetric platform achieves a detection limit of 9.69 × 10 −11 mol/L for XOE, thereby realizing ultrasensitive detection and visual analysis of analytes in shrimp and fish food samples. These results demonstrate its strong application potential in complex practical applications.
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产品关联
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Ningyi Chen,Bingcai Pan
Toxic metal-organic complexes are ubiquitous in contaminated waters but more challenging than free ion counterparts for water decontamination. Currently, almost all the available analytical methods, including Au nanoparticles-mediated colorimetric assay, cannot discriminate metal-organic complexes from the total metal content, which greatly impedes the development of specific decontamination strategies for metal-organic complexes. In this study, we found that nitrilotriacetic acid (NTA) functionalized AuNPs could be used to determine various Cr(III) complexes (Cr(III)-lactate, Cr(III)-malonate, Cr(III)-oxalate, Cr(III)-tartrate, Cr(III)-citrate and Cr(III)-EDTA) in water. Mechanism study indicated that at acidic pHs (pH = 1.6), the protonation of organic ligands resulted in partial dissociation of Cr(III) from the corresponding complexes to expose its accessible orbitals for re-coordination with NTA. Such re-coordination induces the aggregation of the dispersed NTA-AuNPs and thereafter causes considerable changes both in color and UV–Vis spectrum of the solution. The nanosensor is highly selective to Cr(III)-organic complexes with detection limits of 0.029 μM for Cr(III)-lactate, 0.043 μM for Cr(III)-malonate, 0.35 μM for Cr(III)-oxalate, 0.079 μM for Cr(III)-tartrate, 2.58 μM for Cr(III)-citrate and 0.90 μM for Cr(III)-EDTA, respectively. The feasibility of the proposed colorimetric assay has been verified by using the authentic natural and industrial water samples. We believe this study will be of fundamental significance to push forward the application of Au NPs colorimetric assay in authentic waters contaminated by toxic metals.
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高影响因子
ACS Sustainable Chemistry & Engineering
·
2018
·
IF 6.14
Wei Yuan,Zhen Zhang,Xiaoling Cui,Huarong Liu,Chen Tai,Yuanrui Song
Hollow mesoporous CdS@TiO2@Au microspheres as a photocatalyst have been successfully synthesized by γ-irradiation deposition of Au nanoparticles (NPs) on the double shell of hollow CdS@TiO2 microspheres, which are prepared via the “hard core template” approach combining with calcination and solvothermal reaction. The morphology, structure, and properties of the as-prepared hybrid photocatalyst have been characterized by TEM, XRD, XPS, N2 adsorption–desorption, UV–vis DRS, PL, and photoelectrochemical measurements. The results show that the hollow mesoporous double-shell structure is formed with a fluffy TiO2 coating on the surface of the CdS shell composed of stacked CdS NPs. This ternary hybrid photocatalyst exhibits excellent photocatalytic activity for hydrogen evolution and stability under visible-light illumination, which is attributed to the unique ternary hollow mesoporous double-shell heterostructure that may extend the light responsive region, enhance the light-harvesting efficiency, prevent the direct contact between the CdS shell and O2 and H2O, facilitate mass transfer, reduce the recombination rate of charge carriers, and promote the efficiency of water splitting H2 evolution ultimately. The photocatalytic hydrogen generation rate (1720 μmol·g–1·h–1) of the hollow CdS@TiO2@Au microspheres is 2.37 and 12.7 times higher than those of hollow CdS@TiO2 and CdS microspheres, respectively. The loading amount of Au NPs also influences the photocatalytic hydrogen evolution activity of the ternary photocatalyst. Our work expands the application of hybrid hollow microsphere photocatalysts in photocatalytic hydrogen generation by the rational design for enhancing photocatalytic activity under visible light.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xia Yan,Hang Zhao,Hongyan Song,Jun Ma,Xiaofeng Shi
Surface-enhanced Raman scattering (SERS) has great potential for the detection of marine pollutants, but it is still restricted in ultra-trace and quantitative analysis. Here, a strategy for the detection of polycyclic aromatic hydrocarbons (PAHs) was proposed based on Au nanoscale convex polyhedrons (Au NCPs) coated with high-energy facets and embedded with 4-ATP as a probe molecule. Au NCPs acted as SERS substrates and led to limits of detection (LODs) for six common PAHs that reached 0.01 nM. Using internal calibration, the relative standard deviations (RSD) of the spectral stability and reproducibility were as low as 3.36% and 5.11%, respectively. The maximum mean relative errors (AREs) of the predicted and true values were 6.28%. The results indicate that the resulting Au NCPs improved the ultra-trace and quantitative detection of SERS, thus suggesting that the Au NCPs have practical application value in SERS.
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高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 2
·140
·
IF 4.034
Wenjuan Li,Junhuai Xiang,Dandan Men,Honghua Zhang
A novel glucose sensor was reported that consisted of two-dimensional (2D) Au nanosphere arrays and glucose-responsive hydrogel film. This sensor exhibited an intense diffraction signal and an obvious diffraction color on a quartz slide due to the strong diffraction intensity of the Au nanosphere arrays. Thus, glucose was detected via the variation of diffraction wavelength and diffraction color, without a high reflective mirror. In addition, by introducing poly(vinyl alcohol) (PVA) to crosslink the phenylboronic acid (PBA)-modified hydrogel film, the diffraction wavelength of the 2D Au nanosphere arrays/hydrogel composite film shifted in the same direction in high ionic strength condition. In particular, it showed a nearly linear red-shift when the glucose concentration increased from 0 mM to 20 mM. Moreover, this glucose sensor displayed good reproducibility. The nearly linear response and good reproducibility were highly helpful for improving practical application of this glucose sensor.
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