Food Chemistry-X
·
2026
·
IF 9.2
Rong Ma,Chen Chen,Zihang Shi,Yangyang Hu,Yangying Sun,Qiang Xia,Changyu Zhou,Daodong Pan
Although pulsed electric field (PEF) and L-arginine alone can improve meat tenderness, their synergistic molecular mechanism remains unclear. This study first explored the tenderization mechanism of combined PEF and L-arginine (LP) in goose meat. Results showed that LP reduced goose meat shear force by 51.30%, with effects significantly superior to either treatment alone. Hematoxylin-eosin staining revealed that LP enlarged intermuscular spaces. Infrared spectroscopy indicated that LP induced a conformational shift from α-helix to β-sheet in myofibrillar proteins (MPs). Fluorescence spectroscopy showed a significantly increased fluorescence intensity, confirming structural unfolding of MPs. Enzyme activity assays showed that LP elevated calpain activity by 64.33%. SDS-PAGE further confirmed the accelerated degradation of troponin-T. Molecular dynamics simulations revealed that PEF primarily drives the restructuring of the enzyme-substrate binding interface, while L-arginine stabilizes the process by maintaining the hydrogen bond network. This study provides a theoretical basis for developing efficient and controllable meat tenderization technologies.
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PLANT CELL TISSUE AND ORGAN CULTURE
·
2026
·Vol. 165
·期 1
·12
·
IF 2.8
Erst Anna A.,Kotsupiy Olga V.,Tomoshevich Maria A.,Banaev Evgeny V.
For the first time, a mixoploid plants and callus cultures of Rhodiola rosea L. were established. Flow cytometry analysis revealed that seed treatment with 1000 µM colchicine for 24 hours induced mixoploidy in 30% of the samples, while the remaining 70% remained diploid. The combination of 5 µM thidiazuron and 5 µM α-naphthaleneacetic acid resulted in a 100% callus induction rate from cotyledonary leaves and supported callus growth up to 50 g/L dry weight. The phenylpropanoids identified in the rhizomes of in vitro plants (control, 2n) were rosavin, rosin, unidentified phenylpropanoids (PP1, PP2, and PP3), and cinnamyl alcohol. In the mixoploid calluses, the same phenylpropanoids were detected, with the exception of PP1. In the diploid calluses, no phenylpropanoids were detectable within the sensitivity limits of the analytical method used. ANOVA confirmed the significant influence of both UV exposure and cinnamyl alcohol concentration on phenylpropanoid accumulation in mixoploid calluses, with levels reaching up to 484 mg/100 g dry weight. Rosin was identified as the major phenylpropanoid in mixoploid calluses under standard conditions. However, after 72 hours of UV irradiation and cinnamyl alcohol supplementation, PP3 became the dominant compound. This study demonstrates that combining polyploidization, biotransformation, and elicitation is a promising alternative strategy for enhancing metabolite production in Rhodiola rosea cell cultures. Moreover, the targeted application of these stress factors can serve as a tool for ‘metabolic engineering’ in its callus culture. This approach may facilitate the sequential scale-up of metabolite biosynthesis in bioreactors to meet industrial demands.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Lin Zhang,Ruifen Zhang,Mingwei Zhang,Zhenlin Xu,Fei Huang,Xuchao Jia,Lihong Dong,Zhiming Wang,Mei Deng
A debittering method by multi-enzyme coupled hydrolysis for Shatianyu ( Citrus grandis L. Osbeck) whole fruit powder (SWFP) was established. Shatianyu whole fruit homogenate (SWFH) was hydrolyzed by pectinase and cellulase (1:1, w /w, pH 4.5, 50 °C for 2 h). The mixture was then hydrolyzed by naringinase under the optimal conditions: naringinase addition amount of 0.38% ( w /w FW), hydrolysis time for 3.0 h, and hydrolysis temperature at 50 °C. Subsequently, the hydrolyzed products were freeze dried to obtain PCNE-SWFP. Compared with SWFP, the major bitter flavonoids including naringin, melitidin and cigranoside B in PCNE-SWFP were degraded by 40.1%, 37.9% and 49.7%, respectively, and bitterness intensity was reduced by 68.7%. Notably, PCNE-SWFP still showed high total flavonoid content (18.2 mg CE/g DW), also exhibited an increased soluble dietary fiber content, antioxidant activity, as well as water adsorption, water swelling and oil adsorption capacity compared with SWFP.
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Journal of Food Measurement and Characterization
·
2026
·
IF 3.6
He Siqi,Chu Guangru,Wang Aijiao,Cui Liying,Wang Hongji,Liu Shengkai,Liu Zhiguo
Black corn ( Zea mays L.) cobs, an underutilized agricultural by-product, are a rich natural source of anthocyanins. In this study, a green and efficient extraction strategy was developed by integrating microwave-assisted extraction (MAE) with a ternary deep eutectic solvent (DES) composed of choline chloride, citric acid, and 1,4-butanediol (2:1:1, molar ratio). Process variables were optimized using response surface methodology, yielding a maximum total anthocyanin content (TAC) of 13.96 mg/g DW and a cyanidin-3-O-glucoside (C3G) yield of 3.62 mg/g DW. LC-MS/MS profiling identified 19 structurally diverse anthocyanin derivatives in the black corn cob extract. Compared with ethanol, the DES system significantly improved thermal stability, exhibiting slower degradation kinetics and greater pigment retention during heat treatment. Antioxidant assays (DPPH and ABTS⁺) showed markedly higher radical scavenging activity in DES-derived anthocyanins. Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) further revealed that DES treatment disrupted the lignocellulosic cell wall matrix, facilitating more complete release of phenolic compounds than ethanol extraction. These findings highlight ternary DES-MAE as a sustainable and effective approach for valorizing black corn cobs and producing stable, high-value natural pigments for applications in food, cosmetics, and pharmaceuticals. Graphical
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BMC Veterinary Research
·
2026
·
IF 3.1
Yan Xiao,Yang Bowen,Mi Jiali,Zhi Shaoyang,Feng Junchang,Cheng Lijiao,Wang Junli,Qin Chaobin,Nie Guoxing
Background This study was designed to investigate how three distinct bile acids (BAs) modulate glycolipid metabolic disorders and hepatointestinal injury induced by excessive intake of lipids and carbohydrates in Yellow River carp ( Cyprinus carpio L.) and elucidate the underlying mechanisms involved. Here, the fish were randomly assigned to five groups: a control group (CON), a high-fat high-carbohydrate diet (HFHC) group, a HFHC + 300 mg/kg chenodeoxycholic acid (CDCA) group, a HFHC + 300 mg/kg ursodeoxycholic acid (UDCA) group and a HFHC + 300 mg/kg hyodeoxycholic acid (HDCA) group. Results The results revealed that the serum triglyceride, glucose, and total cholesterol levels were significantly elevated in HFHC-fed fish, accompanied by increased glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) activities in the serum and hepatopancreas. However, dietary supplementation with bile acids in the HFHC diet significantly improved these negative changes. Analysis of BA-glycolipid metabolism-related gene expression and enzyme activities in the hepatopancreas revealed that CDCA and HDCA inhibited gluconeogenesis (FBPase/PEPCK/G6Pase) and lipogenesis (SREBP-1/FAS), while promoting glycogen accumulation (genes and glycogen levels) and fatty acid β-oxidation (PPARα) via activation of the FXR (farnesoid X receptor) /SHP (small heterodimer partner) pathway. In contrast, dietary UDCA supplementation increased intestinal TGR5 (takeda G protein-coupled receptor 5) expression and suppressed the activities of two key gluconeogenic enzymes, PEPCK and G6Pase. Additionally, dietary BAs supplementation alleviated HFHC diet-induced intestinal inflammation by inhibiting the NF-κB (Nuclear Factor κB) pathway. Bile acids relieved gut dysbiosis, improved microbial alpha diversity and community structure, and enriched beneficial bacteria including Cetobacterium somerae . These microbial changes eventually modulated host substance synthesis and metabolism. HE staining showed that HFHC diet caused hepatopancreatic lesions and intestinal morphological damage in Yellow River carp, which were effectively alleviated by bile acid addition. Conclusions In conclusion, HFHC diets disrupt fish glycolipid metabolism and impair hepato-intestinal health in Yellow River carp, whereas dietary BAs can attenuate these detrimental effects by modulating metabolic pathways and the gut microbiota composition. Graphical
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Jiayi Song,Maomao Ma,Yijian Wu,Yan Hu,Xinyi Wu,Peipei Guan,Bin Zeng
Green mold, caused by Penicillium digitatum , is one of the most destructive postharvest diseases of citrus fruits, leading to significant rot and economic losses. In this study, the antifungal activity and underlying mechanism of the novel antimicrobial peptide (AMP) N1–6L7A against P. digitatum were investigated. Additionally, the potential application of N1–6L7A for controlling postharvest green mold in Citrus sinensis (L.) Osbeck was examined. N1–6L7A exhibited potential antifungal efficacy by inhibiting both mycelial growth and spore germination of P. digitatum . Furthermore, it demonstrated excellent thermal stability, pH tolerance, and biosafety. The results of fluorescence microscopy assay, cell component leakage, and microscopic observations revealed that N1–6L7A severely damaged the fungal membrane of P. digitatum , leading to the release of intracellular nucleic acids and proteins. N1–6L7A treatment also induced excessive intracellular reactive oxygen species (ROS) production, collapse of mitochondrial membrane potential, and decreased activities of malate dehydrogenase (MDH) and ATPase. Gel retardation assay indicated that P. digitatum -DNA was a target of N1–6L7A. Furthermore, transcriptomic analysis suggested that N1–6L7A might inhibit the growth of P. digitatum by blocking the expression of genes related to the ribosome, amino acid metabolism, and pyrimidine metabolism. Meanwhile, the peptide potentially promoted the expression of genes involved in steroid biosynthesis. Notably, in vivo assays confirmed that N1–6L7A effectively controlled P. digitatum infection in citrus fruits and enhanced host resistance by activating defense-related enzymes. Collectively, our results suggested that N1–6L7A could be used as an alternative fungicide to prevent green mold in citrus fruits.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Hongbo Li,Chen Li,Geng Long,Tian Su,Shuhua Zhang,Yang Zou,Xiaohong Chen,Hongjuan Li,Jinghua Yu
HA-SH-L-P is a novel redox-responsive hydrogel delivery system designed to enhance probiotic colonization, survival, and targeted delivery in the gastrointestinal tract through the synergistic interaction between l -glutamine and probiotics. This interaction not only improves probiotic viability but also modulates the immune microenvironment and strengthens intestinal barrier function. Using Limosilactobacillus fermentum CECT5716 as a model strain, the hydrogel demonstrated stable mechanical properties and responded to hydrogen sulfide (H₂S) in the intestinal microenvironment, enabling rapid probiotic release. In vitro, HA-SH-L-P significantly increased probiotic survival (by 3.56 log CFU/mL compared to free strains). In vivo, HA-SH-L-P modulated immune responses, maintained intestinal barrier integrity, and stabilized the gut microbiota. These results highlight the exceptional performance of HA-SH-L-P as a probiotic delivery system, offering a new approach for the treatment of related diseases.
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高影响因子
JOURNAL OF FOOD SCIENCE
·
2026
·Vol. 91
·期 4
·e71037
·
IF 4.1
Xiaoliang Zhao,Haidong Wen,Yuanyuan Ma,Lixia Xiong,Yuxin Wang,Cunjin Wang,Jing Zhang,Pengcheng Shao,Yuhui Zhao,Ji Zhang,Weijie Zhang
Olive leaves, a major by-product of olive oil production, are an underutilized source of bioactive polysaccharides (olive leaves polysaccharide [OLP]). This study optimized the ultrasonic-assisted extraction (UAE) of OLP using response surface methodology, maximizing yield (4.28%) under conditions of a 1:30 g/mL solid–liquid ratio, 600 W power, and 3h extraction. The extracted polysaccharides (U-OLP) were chemically modified via phosphorylation, sulfation, and selenation. Structural analyses (Fourier transform infrared, UV, nuclear magnetic resonance) confirmed the successful introduction of functional groups. Monosaccharide composition analysis showed that both native and modified U-OLP contained the same eight monosaccharides (Man, GlcN, Rha, GlcA, GalA, Glc, Gal, and Ara), albeit in different proportions. Bioactivity assessments revealed that all modified derivatives, especially the sulfated form (U-OLP-S), exhibited significantly enhanced antioxidant (scavenging 1,1-diphenyl-2-picryl-hydrazyl, hydroxyl, and superoxide radicals) and hypoglycemic activities (inhibiting α-glucosidase and α-amylase) compared to the native U-OLP. Furthermore, in vitro cell assays demonstrated that U-OLP and its derivatives inhibited HepG2 cell proliferation and stimulated proliferation, phagocytosis, and nitric oxide release in RAW264.7 macrophages, with U-OLP-S showing the most potent immunomodulatory effects. The findings confirm that chemical modification, particularly sulfation, is a highly effective strategy for enhancing the bioactivity of olive leaf polysaccharides, broadening their application potential in functional foods and pharmaceuticals.
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CyTA-Journal of Food
·
2026
·
IF 3
Shihu Su,Liping Xiang
A rapid UPLC-FLR method was developed for simultaneous quantification of five major capsaicinoids in chili peppers. Separation of nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin II, and homodihydrocapsaicin I was achieved within 5 min using a Waters HSS T3 column with optimized QuEChERS preparation. The method showed good linearity (r ≥ 0.9902), with LODs of 0.10-0.28 mg/kg, LOQs of 0.35-0.92 mg/kg, and recoveries of 64.5-118.4% (RSD ≤ 10%). Applied to nine Zunyi chili samples from seven varieties, total capsaicinoid content ranged from 11.05 to 4532.50 mg/kg dry weight, revealing substantial differences among varieties and geographical origins. This method is suitable for rapid and accurate analysis of multiple capsaicinoids in chili peppers and related products.
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FOOD CHEMISTRY
·
2026
·
IF 9.8
Jia-Qi Lyu,Jiang-Tao Xu,Hui-Ting Hu,Zhencheng Chen,Guo-Cheng Han,Xiao-Zhen Feng,Heinz-Bernhard Kraatz
An electrochemical detection method was proposed for the detection of Melatonin (MT) based on the synergistic signal amplification of L-Cys and core-shell Cu@Pt bimetallic nanoparticles (Cu@PtNPs). The successful fabrication of the PL-Cys/Cu@PtNPs/GCE was confirmed by characterization techniques including SEM, XRD, and TEM. The method exhibited excellent analytical performance, with a low detection limit of 0.03 μmol/L and a wide linear range across two concentration intervals: 0.1–100.0 μmol/L and 100.0–900.0 μmol/L. The method's practical applicability was successfully validated in human serum and various food samples, achieving satisfactory recovery rates of 98.73 %–99.67 % and 99.49 %–108.57 %, respectively, with relative standard deviations below 6.85 %. The novelty of this work lies in the synergistic amplification effect of the Cu@PtNPs and L-Cys and the application of density functional theory (DFT) calculations to elucidate the MT electrooxidation mechanism, confirming a 2-electron, 1-proton transfer process.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 19
·
IF 4.9
Md. Kamrul Hasan,Jannat Shopan,Israt Jahan,Tonima Islam Suravi
Soil acidity is a divesting factor that restricts crop growth and productivity. Conversely, silicon nanomaterials (Si-NMs) have been praised as a blessing of modern agricultural intensification by overcoming the ecological barrier. Here, we performed a sequential study from seed germination to the yield performance of mustard (Brassica campestris) crops under acid-stressed conditions. The results showed that Si-NMs significantly improved seed germination and seedling growth under acid stress situations. These might be associated with increased antioxidant activity and the preserve ratio of GSH/GSSG and AsA/DHA, which is restricted by soil acidity. Moreover, Si-NMs in field regimes significantly diminished the acid-stress-induced growth inhibitions, as evidenced by increased net photosynthesis and biomass accumulations. Again, Si-NMs triggered all the critical metrics of crop productivity, including the seed oil content. Additionally, Si-NMs, upon dolomite supplementation, further triggered all the metrics of yields related to farming resilience. Therefore, the present study highlighted the crucial roles of Si-NMs in sustainable agricultural expansion and cropping intensification, especially in areas affected by soil acidity.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.6
Yong-qing Liu,Bo-wen An,Tao-Zhu,Rui-yong Jiang,Ye-Wang,Chan Huang,Su-qian Cai,Bi-xiao Zhang,Yan-li Leng,Jing-zhen Pan,Le Liang,Xiao-hua Cai
Rapid and intelligent detection of bioactive molecules and metal ions is vital for health and environmental safety. Herein, a dual-functional nitrogen-doped carbon dots (N-CDs) for L -Histidine ( L -His) and Al 3+ detection was developed employing chitosan (CTS) and umbelliferone (UMB) as the substrates. The N-CDs exhibited a distinct "turn-on" fluorescence response to L -His and a subsequent signal recovery towards Al 3+ with low detection limits (LODs) of 0.950 μM and 4.393 μM. The underlying sensing mechanisms were rigorously elucidated via density functional theory (DFT) calculations and independent gradient model based on Hirshfeld partition (IGMH) analyses, highlighting the pivotal role of surface functional groups. The practical applicants of N-CDs were demonstrated through the fabrication of fluorescent hydrogels, solid-state films for light-emitting diodes (LEDs), and anti-counterfeiting patterns. Notably, a portable and intelligent sensing platform driven by STM32 microcontroller was engineered to capture and analyze RGB signals to realize smart and on-site quantification of L -His, which provided a robust and cost-effective strategy for real-time monitoring of analytes in complex matrices.
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