Experimental and Therapeutic Medicine
·
2019
·Vol. 17
·期 4
·2821-2829
·
IF 1.448
Liqiang Wang,Tonghai Dou,Shuguang Li,Yang Liu
Phthalates are confirmed to have toxic effects on the reproductive system and are likely to have further damaging actions in humans. The present study explored the molecular mechanisms of the toxic effect of mono‑(2‑ethylhexyl) phthalate (MEHP) on mouse Sertoli cells. Cell apoptosis and proliferation assays were used to assess the effects of MEHP on the TM4 Sertoli cell line derived from mouse testes. TM4 cells were treated with two doses of MEHP or left untreated as a control group, followed by RNA extraction and analysis using high‑throughput transcriptome sequencing technology. The gene expression profile obtained was then subjected to a bioinformatics analysis to explore the molecular mechanisms of reproductive toxicity. The results revealed that 528 and 269 genes were upregulated in the high‑ and low‑dose MEHP groups of cells compared with the control group, while 148 and 173 genes were downregulated. Gene ontology (GO) analysis indicated that the differently expressed genes were associated with the GO term ‘extracellular region’ of the cellular component domain in the high and low MEHP groups. Compared with the control group, eight common pathway changes were identified in the high‑ and low‑dose MEHP groups, including ‘terpenoid backbone biosynthesis’. Reverse transcription‑quantitative polymerase chain reaction analysis was used to validation, and hermetic effects were observed for certain genes. These results provide an important basis and experimental data for further research into the mechanisms of phthalate‑induced toxicity.
✓ 已验证
DOI
PubMed
高影响因子
FERROELECTRICS
·
2019
·
IF 0.697
Yan Ming,Hao Hu,Yao Chen,Zhao-Qian Li,You-Qing Shen,Hai-Lin Cong,Bing Yu
We reported a new method for preparing coated capillaries in this paper. First, we combined hydroxypropyl cellulose (HPC) and 2-Methacryloyloxyethyl phosphorylcholine (MPC) to form hydroxypropyl cellulose-poly (2-Methacryloyloxyethyl phosphorylcholine) (HPC-PMPC) by atom transfer radical polymerization (ATRP). Then the photosensitive polymer diazoresin (DR) was used as a linker to the inner wall of the capillary, and finally exposed to ultraviolet light to form a stable covalent bond coating. Protein adsorption on the inner surface of capillary was effectively inhibited. The cytochrome c (Cyt-c), lysozyme (Lys), ribonuclease A (RNase A) and bovine serum albumin (BSA) were separated obviously using capillary electrophoresis (CE) within 15 minutes and showed good stability and repeatability.
✓ 已验证
产品关联
DOI
高影响因子
BIOCATALYSIS AND BIOTRANSFORMATION
·
2019
·
IF 1.627
Lu Dong,Yongkai Xu,Yun Zhang,Aijun Sun,Yunfeng Hu
Chiral 1-phenylethanol and its ester derivative are important chiral chemicals in diverse industries and the preparation of those optically pure enantiomers is of great importance. One bacterium, Bacillus sp. DL-2, whose extracellular proteases could efficiently asymmetrically hydrolyse (±)-1-phenylethyl acetate, was isolated from the deep sea of the Western Pacific. After the immobilization of extracellular proteases and the optimization of enzymatic reactions, (R)-1-phenylethanol was prepared with the enantiomeric excess (e.e.) being 97% and the yield being 41%, respectively. The optimal resolution reaction condition for the preparation of (R)-1-phenylethanol using immobilized extracellular proteases was found to be 5-mM (±)-1-phenylethyl acetate, 360 mg/mL immobilized extracellular proteases, pH 6.5, and 20 °C for 2 h. (S)-1-phenylethyl acetate was generated through enzymatic kinetic resolution with the e.e. being as high as 99% and the yield being 71%, respectively. The optimal resolution reaction condition for the preparation of (S)-1-phenylethyl acetate was found to be 2.5-mM (±)-1-phenylethyl acetate, 440 mg/mL immobilized extracellular proteases, pH 7.5, and 35 °C for 10 h. Our report is the first report about the kinetic resolution of (±)-1-phenylethyl acetate using proteases and the enantio-preference of the proteases was found to be the same as those of most other esterases/lipases. Also notably, the optical purity of (S)-1-phenylethyl acetate generated through kinetic resolution using the proteases of Bacillus sp. DL-2 was the highest report so far. Proteases from deep-sea Bacillus sp. DL-2 are new contributions to the biocatalyst library for the preparation of valuable chiral alcohols and chiral esters through kinetic resolution.
✓ 已验证
产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2019
·
IF 2.883
Xinyun Xu,Jiahao Wang,Ting Yu,Haohan Nian,Hui Zhang,Guangli Wang,Feng Li
Aryloxyphenoxypropionate (AOPP) herbicides with a chiral center are widely used to selectively remove annual and perennial grasses. However, the study of the enzymes involved in enantioselective degradation of AOPP herbicides is limited. A novel family VIII carboxylesterase gene, estwx , hydrolyzed the ester bond cleavage of AOPP herbicides to form the corresponding acid and alkyl side chain alcohol, was cloned from Brevundimonas sp. strain QPT-2 and overexpressed in E. coli BL21. The purified recombinant EstWX was shown to hydrolyze a wide range of AOPP herbicides with various catalytic efficiencies. The enantioselectivity assay indicated that EstWX preferentially catalyzed the hydrolysis of the R -enantiomer of AOPP herbicides. The S73, K76, Y196 together with W368 residues, especially S73 and Y196, were essential for the catalytic function of the carboxylesterase. EstWX is a promising candidate for the bioremediation of multiple AOPP herbicide-contaminated environments and for future mechanism studies on the enantioselective degradation of chiral AOPP herbicides.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Xiaofeng Tan,Xiaoying Wang,Lianhua Zhang,Luyao Liu,Gengxiu Zheng,He Li,Feimeng Zhou
Polydopamine (PDA)-coated or encapsulating Cu3(PO4)2 (Cu3(PO4)2@PDA) nanosheets were synthesized, allowing the C-reaction protein (CRP) antibody to be attached electrostatically for immunosensing of CRP with simple photothermal detection. The antibody-covered Cu3(PO4)2@PDA nanosheets replace the antibody-conjugated enzyme in the enzyme-linked immunosorbant assays. Owing to the high surface area of the 2-D-structured Cu3(PO4)2@PDA nanosheets and the coabsorption of light in the near-IR spectrum by Cu3(PO4)2 and PDA, a small amount of Cu3(PO4)2@PDA confined in the wells of a titer plate generates an easily detectable temperature change after irradiation at 808 nm. The temperature changes, measured by an inexpensive pen-type thermometer, increased linearly with the analyte concentration from 0.42 to 16 pM. We found that the linear relationship can be fitted by the isotherm derived from responses collected from heterogeneous sensors covered with different ligand or antibody densities. The low detection limit (0.11 pM) is largely due to the attachment of a great number of antibodies onto the flat nanosheets. The antibody-covered Cu3(PO4)2@PDA nanosheets are stable and can be used under conditions that are generally unfavorable to enzymatic activities. The excellent agreement between our results and immunoturbidimetric assays of CRP in serum samples from patients and healthy donors demonstrates its utility for disease diagnosis in clinical settings. This cost-effective, biocompatible, and convenient photothermal immunosensor affords a range of possibilities for detecting diverse protein biomarkers.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Zhi-Hui Huang,Zhi-Yong Ji,Ying-Ying Zhao,Jie Liu,Fei Li,Jun-Sheng Yuan
Both thermal and alkali are effective means of persulfate (PS) activation, and the synergistic effect of them should achieve a great degradation result. It is evaluated for treating coal chemistry industry wastewater because the by-product produced in the process is sulfate, which is pre-existing in the wastewater. With thermal and alkali synergistic activation, the application of the response surface method revealed the effects of temperature, pH and concentration of persulfate on the degradation ratio of 2-methoxyphenol (MOP, a typical contaminant in coal chemical industry wastewater) and their interactions. The results showed that the degradation rate of the contaminant was temperature and pH dependent, while chloride (Cl − ) and sulfate (SO 4 2− ) ions had no obvious negative effect. The degradation ratio could be enhanced with higher activation temperature and pH (7–12). The faster degradation could be achieved by thermal-alkali synergistic activation, and the degradation ratio of 88.4% and 93.8% could be achieved at pH = 12 within 10 min and 20 min, respectively. Electron spin resonance (ESR) spectra and free radical scavenging experiments indicated that MOP degradation was achieved by the oxidation of both sulfate radical (SO 4 − ) and hydroxyl radical ( OH) at pH = 6, and only by OH at pH = 12. In addition, the radical reaction was intense under thermal-alkali synergistic activation of PS, while the quenching reaction was ineffective.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry A
·
2019
·Vol. 8
·期 1
·369-379
·
IF 10.733
Chun Li,Lingling Shi,Lili Zhang,Peng Chen,Junwu Zhu,Xin Wang,Yongsheng Fu
Organic two-dimensional (2D) materials with unique optic-electronic properties are attracting great interest for application in functional electronic devices. However, low electronic conductivity and poor stability still are major defects of 2D organic systems. Herein, we demonstrated ultrathin 2D conductive cobalt–hexaaminobenzene metal–organic coordination polymer nanosheets (Co–HAB-NSs) with a thickness of ∼4.5 nm. The as-prepared Co–HAB-NSs exhibit a low overpotential (310 mV @ 10 mA cm−2) and high durability in 1 M KOH. The experimental and computational results show that the high electrocatalytic activity is ascribed to the enhanced electrochemically active surface area as well as the dense and stable catalytic active sites of Co–HAB-NSs.
✓ 已验证
产品关联
DOI
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2018
·
IF 3.457
Jian Yin,Ge Gong,Chao Sun,Zhaoyang Yin,Chao Zhu,Bin Wang,Qin Hu,Yuerong Zhu,Xinhui Liu
Angiogenesis plays a key role in the repair of large segmental bone defects with tissue-engineered bones. However, there is no effective method of promoting angiogenesis in tissue-engineered bone. Both angiopoietin 2 (Ang2) and autophagy have been shown to be involved in angiogenesis, but their roles in angiogenesis of tissue-engineered bone remains unknown. In this in vivo study, a radius bone defect was created in New Zealand white rabbits, which were then treated by implantation of a hydroxyapatite/collagen scaffold followed by injection of different concentrations of Ang2. Expression of the autophagic modulators microtubule-associated protein 1 light chain 3 (LC3), Beclin-1, and SQSTM1/P62 were measured via western blotting, while the angiogenic modulators VEGF and CD31 were detected by western blotting and immunohistochemistry, respectively. X-ray imaging combined with general observation was used to evaluate bone defect healing. Expression of LC3 -I/LC3-II, Beclin-1, VEGF, and CD31 in the callus area increased and SQSTM1/p62 decreased in a dose-dependent manner with increasing Ang2 concentration. In the group treated with a high concentration of Ang2, the new callus grew well, accompanied by remarkable angiogenesis, leading to good repair of the bone defects. However, in the low concentration of Ang2 group, in spite of the existence of angiogenesis and new bone formation, the bone defects were not repaired. Furthermore, angiogenesis and osteogenesis were both obstructed in the control group. In conclusion, our study demonstrated that a high concentration of Ang2 promoted angiogenesis in tissue-engineered bone and improved repair of bone defects by inducing autophagy.
✓ 已验证
DOI
PubMed
高影响因子
Cancer Cell International
·
2018
·Vol. 18
·期 1
·1-10
·
IF 3.96
Zhong Banghua,Wang Kewei,Xu Hao,Kong Fanmin
Background Formin-like 2 (FMNL2) is a member of Formin family which governs cytokinesis, cellular polarity and morphogenesis. Dysregulation of FMNL2 has been discovered in cancers and is closely related to cancers. However, the role of FMNL2 in gastric cancer remains unclear. In this study, we aimed to investigate the role of FMNL2 in gastric cancer cells. Methods A FMNL2-specific shRNA was employed to decrease the endogenous expression of FMNL2. Then the degree of proliferation, apoptosis, migration and invasion of gastric cancer cells was assessed by MTT assay, flow cytometry, wound healing assay and transwell assay, respectively. The expression and distribution of FMNL2 and protein kinase C (PKC) α was detected by immunofluorescence. The internalization of integrins was detected by enzyme-linked immunosorbent assay. Results Our results showed that silencing FMNL2 suppressed proliferation, migration and invasion, and induced apoptosis of gastric cancer cells. The integrin internalization induced by PKC was declined by FMNL2 silencing. Conclusions Our study reveals that silencing FMNL2 suppresses growth and metastasis of gastric cancer cells. Modulation on integrin internalization may be implicated in the role of FMNL2 in growth and migration of gastric cancer cells. Our study indicates that FMNL2 may become a potential therapeutic target for gastric cancer.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Minerals
·
2018
·Vol. 9
·期 1
·15
·
IF 1.835
Bin Luo,Junbo Liu,Quanjun Liu,Chao Song,Li Yu,Shimei Li,Hao Lai
Zinc is mostly extracted from oxidized zinc and zinc sulfide minerals, and this process involves flotation as a key step. While it is easier to float the sulfide mineral, its consumption and depletion has led to an increased reliance on oxidized zinc minerals, including smithsonite. Hence, the development of efficient ways of collecting smithsonite by flotation is an important objective. Herein, we describe the use of 2-(hexadecanoylamino)acetic acid (HAA), a novel surfactant, as a collector during smithsonite flotation. The mechanism and flotation performance of HAA during smithsonite flotation was investigated by total organic carbon (TOC) content studies, zeta potential measurements, Fourier-transform infrared (FTIR) spectroscopy, and X-ray photoelectron spectroscopy (XPS) analyses, combined with microflotation experiments. The flotation results revealed that HAA was an excellent collector in pulp over a wide pH range (9–12) and at a relatively low concentration (2 × 10−4mol/L), at which a recovery of close to 90% of the smithsonite mineral was obtained. TOC content studies revealed that the good flotation recovery was ascribable to large amounts of collector molecule adsorbed on the smithsonite surface, while zeta potential measurements showed that the HAA was chemically adsorbed onto the smithsonite. FTIR and XPS analyses revealed that the HAA collector molecules adsorbed onto the smithsonite surface as zinc–HAA complexes involving carboxylate moieties and Zn sites on the smithsonite surface in alkaline solution.Keywords:smithsonite;flotation;2-(hexadecanoylamino)acetic acid;collector;adsorption
✓ 已验证
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2018
·Vol. 99
·期 6
·3192-3202
·
IF 2.379
Zhisheng Zhou,Xing Hu,Guowen Zhang,Rui Wang,Deming Gong
BACKGROUND 5-Hydroxymethyl-2-furaldehyde (5-HMF), a by-product of the Maillard reaction, usually present in fried and baked food, may cause potential harm to the human body. Here, the interaction between 5-HMF and calf thymus DNA (ctDNA) under physiological buffer (pH 7.4) was studied using multi-spectroscopic methods combined with multivariate curve resolution–alternating least squares (MCR-ALS) chemometrics and molecular simulation techniques. RESULTS The concentration profiles and pure spectra of the three components (5-HMF, ctDNA and 5-HMF–ctDNA complex) were extracted from highly overlapping spectra using MCR-ALS analysis, which verified the formation of 5-HMF–ctDNA complex. The binding constant being of the order of 10 3 L mol −1 at four temperatures (292, 298, 304 and 310 K) indicated a weak affinity in the binding of 5-HMF to ctDNA. The binding interaction was mainly driven by hydrogen bonds and van der Waals forces. Viscosity analysis, melting assay, ionic strength effect and competitive fluorescence studies ascertained that 5-HMF bound to ctDNA through groove binding, and it tended to bind to guanine–cytosine rich region of ctDNA which was characterized using Fourier transform infrared spectra and molecular docking. Circular dichroism spectral analysis and DNA cleavage assays indicated that the ctDNA conformation was altered from B to A form and 5-HMF caused DNA damage at higher concentration. CONCLUSIONS The results suggested that 5-HMF bound to ctDNA through groove binding and caused DNA damage. This research may contribute to understand the binding mechanism of 5-HMF to ctDNA and to the assessment of the toxicological effect of 5-HMF in biological processes. © 2018 Society of Chemical Industry
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2018
·
IF 2.955
Weinan Gong,Xiaoshuang Yin,Ying Liu,Yun Chen,Wenzhong Yang
The inhibitory effects of 2-amino-4-(4-methoxyphenyl)-thiazole (MPT) on mild steel corrosion were evaluated in 0.5 M H 2 SO 4 and 1 M HCl solutions, respectively. From the analysis of potentiodynamic polarization curves and electrochemical impedance spectroscopy, MPT can effectively inhibit the corrosion of mild steel in acidic solutions. Under the optimal conditions, the inhibition efficiency ( η ) was as high as 95% in 0.5 M H 2 SO 4 solution. The inhibition property of MPT was much better than that of the reported corrosion inhibitor 2-amino-4-phenylthiazole (APT). Polarization curves also demonstrated that MPT mainly inhibited the anodic corrosion of mild steel in HCl solution and the cathodic corrosion in H 2 SO 4 solution, respectively. The adsorption of MPT on the surface of mild steel was found to obey Langmuir adsorption isotherm in both acid solutions. Quantum calculations suggest that the addition of electron donor group (−OCH 3 ) endowed the molecule better adsorption ability on mild steel surface. The interaction between protonated MPT and anions on Fe (0 0 1) surface was firstly investigated using molecular dynamic stimulations, and the results showed that with the adsorption of sulfate ions, MPTH + had a superior binding energy on the surface of Fe. The investigation of UV–vis spectrum demonstrated the direct correlation of the experimental data and the theoretical calculations.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）