APPLIED AND ENVIRONMENTAL MICROBIOLOGY
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2017
·Vol. 83
·期 10
·
IF 3.807
Lei Yu,Ming-yue Cao,Peng-tao Wang,Shi Wang,Ying-rong Yue,Wen-duo Yuan,Wei-chuan Qiao,Fei Wang,Xin Song
Biohydrogen production from the pulp and paper effluent containing rich lignocellulosic material could be achieved by the fermentation process. Xylose, an important hemicellulose hydrolysis product, is used less efficiently as a substrate for biohydrogen production. Moreover, azo dyes are usually added to fabricate anticounterfeiting paper, which further increases the complexity of wastewater. This study reports that xylose could serve as the sole carbon source for a pure culture of Klebsiella oxytoca GS-4-08 to achieve simultaneous decolorization and biohydrogen production. With 2 g liter−1 of xylose as the substrate, a maximum xylose utilization rate (URxyl) and a hydrogen molar yield (HMY) of 93.99% and 0.259 mol of H2 mol of xylose−1, respectively, were obtained. Biohydrogen kinetics and electron equivalent (e− equiv) balance calculations indicated that methyl red (MR) penetrates and intracellularly inhibits both the pentose phosphate pathway and pyruvate fermentation pathway, while methyl orange (MO) acted independently of the glycolysis and biohydrogen pathway. The data demonstrate that biohydrogen pathways in the presence of azo dyes with sulfonate and carboxyl groups were different, but the azo dyes could be completely reduced during the biohydrogen production period in the presence of MO or MR. The feasibility of hydrogen production from industrial pulp and paper effluent by the strain if the xylose is sufficient was also proved and was not affected by toxic substances which usually exist in such wastewater, except for chlorophenol. This study offers a promising energy-recycling strategy for treating pulp and paper wastewaters, especially for those containing azo dyes.IMPORTANCE The pulp and paper industry is a major industry in many developing countries, and the global market of pulp and paper wastewater treatment is expected to increase by 60% between 2012 and 2020. Such wastewater contains large amounts of refractory contaminants, such as lignin, whose reclamation is considered economically crucial and environmentally friendly. Furthermore, azo dyes are usually added in order to fabricate anticounterfeiting paper, which further increases the complexity of the pulp and paper wastewater. This work may offer a better understanding of biohydrogen production from xylose in the presence of azo dyes and provide a promising energy-recycling method for treating pulp and paper wastewater, especially for those containing azo dyes.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Ting Zhou,Luyao Zang,Junjie Sun,Xia Zhang,Zijie Qu,Xia Liu,Guodong Zhang,Xiufeng Wang,Fang Wang,Zhiqing Zhang
Considering the severe hazard to health posed by nitrophenol (NP) isomers, especially 4-NP, reliable and selective detection strategies are urgently challenged. In this study, a fluorescence platform based on glutathione-stabilized gold/silver nanoclusters (GSH-Au/Ag NCs) and zeolite imidazolate framework material (ZIF-8) was developed for 4-NP detection. The synergistic effect of gold and silver makes the bimetallic nanoclusters (GSH-Au/Ag NCs) much better in optical properties than gold nanoclusters. Subsequently, GSH-Au/Ag NCs with aggregation-induced emission (AIE) effect was encapsulated into ZIF-8 to obtain GSH-Au/Ag NCs@ZIF-8 nanocomposite. Compared with GSH-Au NCs, the fluorescence signal was efficiently further improved nearly 7 times through the confinement effect of ZIF-8, and the fluorescence lifetime was increased from 1.2 μs to 6.9 μs. The addition of 4-NP effectively quenched the fluorescence emission peak of GSH-Au/Ag NCs@ZIF-8 at 600 nm, and the assay exhibited highly sensitivity to 4-NP with the detection limit of 1.11 μM from 2.0 μM to 35 μM. Especially, results showed that 4-NP could be easily discriminated from nitrophenol isomers (3-NP and 2-NP). The mechanism research demonstrated that the absorption curve of GSH-Au/Ag NCs@ZIF-8 + 4-NP overlapped greatly with the fluorescence excitation spectrum of GSH-Au/Ag NCs@ZIF-8, and the fluorescence lifetime of GSH-Au/Ag NCs@ZIF-8 did not change when 4-NP was present or not. Therefore, the mechanism of GSH-Au/Ag NCs@ZIF-8 for 4-NP detection was mainly attributed to the inner filter effect (IFE). Furthermore, the developed detection assay was applied to water sample with satisfactory recovery results, demonstrating its high potential in monitoring 4-NP in environment.
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JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Zenghui Xu,Abudurexiti Reheman,Zhihao Lu,Shuiqiang Yu,Zhiwei Sun,Jinmao You
The detection of aromatic aldehydes, considered potential genotoxic impurities, holds significant importance during drug development and production. Current analytical methods necessitate complex pre-treatment processes and exhibit insufficient specificity and sensitivity. This study presents the utilization of naphthalenediimide as a pre-column derivatisation reagent to detect aromatic aldehyde impurities in pharmaceuticals via high-performance liquid chromatography (HPLC). We screened a series of derivatisation reagents through density functional theory (DFT) and investigated the phenomenon of photoinduced electron transfer (PET) for both the derivatisation reagents and the resulting products. Optimal experimental conditions for derivatisation were achieved at 40 °C for 60 min. This approach has been successfully applied to detect residual aromatic aldehyde genotoxic impurities in various pharmaceutical preparations, including 4-Nitrobenzaldehyde, 2-Nitrobenzaldehyde, 1,4-Benzodioxane-6-aldehyde, and 5-Hydroxymethylfurfural. The pre-column derivatisation method significantly enhanced detection sensitivity and reduced the limit of detection (LOD), which ranged from 0.002 to 0.008 μg/ml for the analytes, with relative standard deviations < 3 %. The correlation coefficient (R 2 ) >0.998 demonstrated high quality. In chloramphenicol eye drops, the concentration of 4-Nitrobenzaldehyde was measured to be 8.6 µg/mL below the specified concentration, with recoveries ranging from 90.0 % to 119.2 %. In comparison to existing methods, our work simplifies the pretreatment process, enhances the sensitivity and specificity of the analysis, and offers comprehensive insights into impurity detection in pharmaceutical preparations.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Binxuan Diao,Fuhao Jiang,Heqing Ye,Rui Wang,Hongjiang Li,Haoran Zhang,Sang Woo Joo,Chenhao Cong,Se Hyun Kim,Xinlin Li
Two-dimensional metal – organic frameworks (2D MOFs) hold great promise as electrochemically active materials. However, their application in MOF nanocomposite electrodes in solution engineering is limited by structural self-stacking and imperfect conductive pathways. In this study, we used meso -tetra(4-carboxyphenyl ) porphine (TCPP) with off-domain π-bonds to reconstitute Zn-TCPP (ZMOF) and poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) through an interfacial modulation strategy involving electrostatic coupling and hydrogen bonding, creating a conductive composite with a nanosheet structure. The negatively charged PSS and ZMOF formed a three-dimensional interconnected conductive network with excellent interfaces. The positively charged PEDOT, fine tuned with the lamellar structure, established strong π-π stacking interactions between the porphyrin and thiophene rings. ZMOF also induced changes in the PEDOT chain structure, weakening PSS entanglement and enhancing charge-transport properties. The specific capacitance of the prepared supercapacitor was as high as 967.8 F g −1 . Flexible supercapacitors produced on a large scale using dispensing printing technology exhibited an energy density of 1.85 μWh cm −2 and a power density of 7.08 μW cm −2 . This interfacial modulation strategy also exhibited excellent wearable properties, with 96 % capacitance retention at a 180° bending angle and stable cycling performance. This study presented a significant advancement in the functionalization of 2D materials, highlighting their potential for device-grade capacitive architectures.
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Materials Today Chemistry
·
2024
·
IF 6.7
Bo Shen,Zhengqiu Chen,Huaming Mao,Jungang Yin,Yu Ren,Wei Dai,Shuanglong Zhao,Xiaoyu Chong,Hongwei Yang
In this study, we report a cuprous oxide nano-framework catalyst with abundant oxygen vacancies and multiple exposed crystalline surfaces. The catalyst exhibits excellent catalytic performance in the hydro-reduction reaction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) with good reusability. Experimental and theoretical simulations showed that the construction of oxygen vacancies induced a change in the corresponding atomic configuration and electronic properties of the catalyst, which contributed to the improvement of the adsorption of 4-NP and the capture and activation of H atoms, thus accelerating the transfer process of reactive hydrogen and electrons to 4-NP using the catalyst as a mediator. In addition, the rough surface of the nano-framework exposed a variety of crystalline facets, among which the (210) facets possessed more suitable H atom adsorption energy and more electron transfer compared to the (100) facet, which further improved the hydrogenation performance of the catalyst. This study provides a catalyst design strategy based on the synergy of oxygen vacancies and crystalline surfaces for the efficient degradation of organic pollutants.
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American Journal of Translational Research
·
2016
·
IF 3.146
Junhui Yuan,Kuang Ge,Junhuan Mu,Jiang Rong,Li Zhang,Bin Wang,Jingyuan Wan,Gong Xia
Ferulic acid (FA), a phenolic acid which is abundant in vegetables and fruits, has been reported to exert anti-oxidative and anti-inflammatory activities. In the present study, the pharmacological effects and the underlying mechanisms of FA in mice with acetaminophen-induced hepatotoxicity were investigated. Our results revealed that FA pretreatment inhibited the augments of serum aminotransferases in a dose-dependent manner and attenuated the hepatic histopathological abnormalities and hepatocellular apoptosis in acetaminophen (APAP) exposed mice. Moreover, FA inhibited the expression of cytochrome P450 2E1 (CYP2E1), enhanced the activities of superoxide dismutase (SOD) and catalase (CAT) as well as the contents of glutathione (GSH). Furthermore, FA markedly attenuated acetaminophen-induced serum tumor necrosis factor (TNF)-α and interleukin (IL)-1β production, suppressed Toll-like receptor (TLR) 4 expression and dampened p38 mitogen-activated (MAPK) and nuclear factor kappa (NF-κB) activation. These data suggested that FA could effectively protect against APAP-induced liver injury by down-regulated expression of CYP 2E1 and the suppression of TLR4-mediated inflammatory responses.
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MOLECULES
·
2024
·Vol. 29
·期 12
·2778
·
IF 4.2
Xi Zhu,Yong Guo,Baozhan Zheng
Efficient recovery of gallium (Ga) from vanadium slag processing residue (VSPR) solution is of great significance for environmental protection and resource utilization, but improving its selective adsorption against the coexisting Sc3+and In3+is still challenging. Herein, a novel adsorbent consisting of 4-amino-3-hydrazino-1,2,4-triazol-5-thiol (AHTZT)-modified graphene oxide (GO-AHTZT) was successfully synthesized that exhibits a higher adsorption selectivity for Ga3+in VSPR solution with coexisting Sc3+and In3+. Under optimal conditions, the adsorption capacity of GO-AHTZT for Ga3+can reach 23.92 mg g−1, which is 4.9 and 12.6 times higher than that for Sc3+(4.87 mg g−1) and In3+(1.90 mg g−1) adsorption, indicating the excellent anti-interference ability of GO-AHTZT against Sc3+and In3+. The process and mechanism of Ga3+adsorption onto GO-AHTZT was also studied and discussed in detail. By measuring the adsorption process and by characterizing the adsorbent before and after adsorption, we demonstrate that the selective interaction between the Ga3+- and N-containing groups in AHTZT is the main reason for the improved adsorption selectivity. This work opens up an avenue for the design and synthesis of highly selective adsorbents for Ga3+in complex VSPR solutions.
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Molecular Catalysis
·
2024
·
IF 3.9
Xuecai Wei,Xiaoyang Yue,Quan Yuan,Ying He,Li Ma,Liya Zhou,Guanhua Liu,Yunting Liu,Yanjun Jiang
Ethyl ( S )-4‑chloro-3-hydroxybutyrate [( S )-CHBE] is attractive as a necessary intermediate for the production of highly valued products in pharmaceutical industries. Nowadays, alcohol dehydrogenases play an increasingly essential role in the asymmetric synthesis of chiral alcohols. In this work, a nicotinamide adenine dinucleotide (NADH)-dependent alcohol dehydrogenase from Pseudomonas bacterium ( Pb ADH) was obtained by genome mining method and identified to exhibit an excellent tolerance against the high substrate and product concentrations. For the regeneration of NADH, Bacillus megaterium glucose dehydrogenase ( Bm GDH) was successfully co-expressed with Pb ADH in E. coli cells. So much as 3.5 M (574 g/L) ethyl 4-chloroacetoacetate (COBE) can be converted to ( S )-CHBE completely with a > 99 % ee value under the catalysis by recombinant cells, even in a monophasic aqueous media with a single batch charge of substrate. In addition, in order to verify the substrate scope of Pb ADH, six other α/β-ketone esters and aromatic ketones were also evaluated as substrates at high substrate concentrations (100–650 g/L), and the corresponding chiral alcohols with highly optical purity were also achieved by co-expressed E. coli cells.
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Animal Nutrition
·
2025
·
IF 7.5
Li Dong,Yangshu Zhou,Mengxuan Wang,Qingqing Yuan,Guangzhi Qiu,Jun Liu,Hongrong Wang,Aimin Wu,Lihuai Yu,Daiwen Chen
This study aims to elucidate intestinal injury from the perspective of intestinal epithelial cell homeostasis and to explore the modulatory roles of terpine-4-ol (a major bioactive component of tea tree oil). Forty 28-day-old piglets (7.82 ± 0.43 kg) were randomly divided into 5 groups with 8 pigs per group. Piglets in the control (CON) and lipopolysaccharide (LPS) groups were fed a basal diet, while those in the remaining 3 groups were fed the basal diet supplemented with 30 (LTP), 60 (MTP), or 90 mg/kg terpine-4-ol (HTP), respectively. Piglets in the LPS and terpine-4-ol supplementation groups were intraperitoneally injected with 100 μg/kg BW of LPS at the end of the 21-day growth period. Six hours after injection, 6 piglets from each group were randomly selected and slaughtered to detect intestinal cell homeostasis. The results showed that the average daily weight gain (ADG) and average daily feed intake (ADFI) of piglets in MTP and HTP groups were higher than those in the CON group ( P < 0.05), whereas the feed conversion ratio (FCR) of piglets in LTP group were lower as compared to the CON group ( P < 0.05). Terpine-4-ol supplementation also decreased the diarrhea rate of piglets. Lipopolysaccharide challenge decreased the villus length and the ratio of villus length to crypt depth (V/C) in the jejunum ( P < 0.05), decreased the V/C ratio and increased the crypt depth in the ileum ( P < 0.05). In contrast, terpine-4-ol supplementation led to increased villus length and V/C ratio in the jejunum, raised the V/C ratio in the HTP group, and decreased crypt depth in the LTP and MTP groups in the ileum ( P < 0.05). Lipopolysaccharide reduced the number of Ki67 + cells and increased the number of caspase 3 + cells ( P < 0.05) in both the jejunum and ileum, whereas terpine-4-ol increased the number of Ki67 + cells in the crypts of the jejunum and ileum, while decreasing the number of caspase 3 + cells of the the ileum and in the jejunum of MTP and HTP groups ( P < 0.05). Lipopolysaccharide down-regulated the expression of the intestinal stem cell marker leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) ( P < 0.05, execpt for Lgr5 gene in the jejunum), while terpine-4-ol supplementation alleviated the effect. Lipopolysaccharide decreased the number of goblet cells and mucin 2 (Muc2) secretion ( P < 0.05), whereas the number of goblet cells and Muc2 secretion in the small intestine of MTP group was increased ( P < 0.05). In addition, LPS injection led to a reduction in the expression of endocrine cell marker chromogranin A and absorption cell marker villin ( P < 0.05). Conversely, terpine-4-ol increased the abundance of endocrine cells and absorption cells in the small intestine ( P <0.05). Moreover, LPS inhibited the expression of genes and proteins of wingless-type MMTV integration site family (Wnt)/β-catenin signaling pathway ( P < 0.05), whereas terpine-4-ol reversed this effect. In conclusion, terpine-4-ol supplementation improved growth performance, decreased diarrhea in piglets and alleviated the damage to the intestinal epithelial cell homeostasis under LPS challenge, probably via Wnt/β-catenin signaling activation. A dose of 60 mg terpine-4-ol per kilogram diet was recommended based on the present study.
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ENVIRONMENTAL POLLUTION
·
2025
·
IF 7.3
Fangyuan Dong,Xiao Chen,Ziming Ye,Xiangyu Deng,Shaoyang Hu,Chunguang Liu
Phenolic halogenated DBPs are an emerging class of highly toxic DBPs in drinking water. However, the underlying mechanisms for isomer-specific cytotoxicity of phenolic disinfection byproducts are still largely veiled. In this work, comparative cytotoxicity of 3,5-dichloro-2-hydroxybenzoic acid (3,5-DC-2-HBA) and 3,5-dichloro-4-hydroxybenzoic acid (3,5-DC-4-HBA) in diverse mammalian cell lines was investigated, SOD activity in Chinese hamster ovary cells treated by 3,5-DC-2-HBA and 3,5-DC-4-HBA was compared, and their binding interactions with Cu/Zn-superoxide dismutase (Cu/Zn-SOD) were analyzed. Results indicated that 3,5-DC-2-HBA commonly presented significantly higher cytotoxicity compared to 3,5-DC-4-HBA. Exposure to 3,5-DC-2-HBA and 3,5-DC-4-HBA caused a dose-dependent increase in intracellular SOD activity, with the trend paralleling the cytotoxicity of the two isomeric compounds. In addition, this rank order was consistently observed in the conformational changes, secondary structure alterations, as well as enzymatic activity modulation of Cu/Zn-SOD induced by these isomeric compounds. Molecular docking simulations further elucidated the binding mechanisms, demonstrating that van der Waals interactions and hydrogen bonding energies predominantly drive complex formation between these two halogenated phenolic DBPs and Cu/Zn-SOD. Notably, the interaction with Cu/Zn-SOD may be generally applied to explain the isomeric structure-derived cytotoxic pattern of various groups of phenolic DBPs. This systematic investigation advances our mechanistic understanding of isomer-specific cytotoxicity in phenolic DBPs, providing valuable insights for risk assessment and mitigation strategies in drinking water treatment processes.
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Inorganic Chemistry Frontiers
·
2025
·
IF 6.4
Wenhao Zhang,Meiyan Cui,Zhiying Zhao,Tianyu Zhu,Zhangzhen He
Based on the crystal structure of A2Cu5(TeO3)(SO4)3(OH)4 (A = Na, K), two new compounds ACo5(SeO3)4(OH)2F (A = Rb, Cs) have been synthesized by introducing F− ions, achieving the extension from a one-dimensional kagomé strip lattice to a distorted 2-uniform lattice (T29). Magnetic measurements indicate that RbCo5 exhibits antiferromagnetic order at low temperatures, while CsCo5 shows canted antiferromagnetic behavior. Both compounds display a field-induced magnetic transition at their respective critical fields. To the best of our knowledge, this is the first reported synthesis of compounds with a distorted 2-uniform lattice (T29).
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Bioactive Materials
·
2023
·
IF 18.9
Zhaochuang Wang,Wenhao Zhang,Guo Bai,Qiaohui Lu,Xiaoyu Li,Yan Zhou,Chi Yang,Yan Xiao,Meidong Lang
Elastomeric scaffolds, individually customized to mimic the structural and mechanical properties of natural tissues have been used for tissue regeneration. In this regard, polyester elastic scaffolds with tunable mechanical properties and exceptional biological properties have been reported to provide mechanical support and structural integrity for tissue repair. Herein, poly(4-methyl- ε -caprolactone) (PMCL) was first double-terminated by alkynylation (PMCL-DY) as a liquid precursor at room temperature. Subsequently, three-dimensional porous scaffolds with custom shapes were fabricated from PMCL-DY via thiol-yne photocrosslinking using a practical salt template method. By manipulating the M n of the precursor, the modulus of compression of the scaffold was easily adjusted. As evidenced by the complete recovery from 90% compression, the rapid recovery rate of >500 mm min −1 , the extremely low energy loss coefficient of <0.1, and the superior fatigue resistance, the PMCL20-DY porous scaffold was confirmed to harbor excellent elastic properties. In addition, the high resilience of the scaffold was confirmed to endow it with a minimally invasive application potential. In vitro testing revealed that the 3D porous scaffold was biocompatible with rat bone marrow stromal cells (BMSCs), inducing BMSCs to differentiate into chondrogenic cells. In addition, the elastic porous scaffold demonstrated good regenerative efficiency in a 12-week rabbit cartilage defect model. Thus, the novel polyester scaffold with adaptable mechanical properties may have extensive applications in soft tissue regeneration.
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