Scientific Reports
·
2017
·Vol. 7
·期 1
·1-12
·
IF 4.259
Zhang Chong,Cao Songxiao,Jin Yazhong,Ju Lijun,Chen Qiang,Xing Qiaojuan,Qi Hongyan
To better understand the function role of the melon CmLOX18 gene in the biosynthesis of C6 volatiles during fruit ripening, we biochemically characterized CmLOX18 and identified its subcellular localization in transgenic tomato plants. Heterologous expression in yeast cells showed that the molecular weight of the CmLOX18 protein was identical to that predicted, and that this enzyme possesseed lipoxygenase activity. Linoleic acid was demonstrated to be the preferred substrate for the purified recombinant CmLOX18 protein, which exhibited optimal catalytic activity at pH 4.5 and 30 °C. Chromatogram analysis of the reaction product indicated that the CmLOX18 protein exhibited positional specificity, as evidenced by its release of only a C-13 oxidized product. Subcellular localization analysis by transient expression in Arabidopsis protoplasts showed that CmLOX18 was localized to non-chloroplast organelles. When the CmLOX18 gene was transgenically expressed in tomato via Agrobacterium tumefaciens -mediated transformation, it was shown to enhance expression levels of the tomato hydroperoxide lyase gene LeHPL , whereas the expression levels of six TomLox genes were little changed. Furthermore, transgenic tomato fruits exhibited increases in the content of the C6 volatiles, namely hexanal, (Z)-3-hexanal, and (Z)-3-hexen-1-ol, indicating that CmLOX18 probably plays an important role in the synthesis of C6 compounds in fruits.
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PubMed
高影响因子
Natural Products and Bioprospecting
·
2016
·Vol. 6
·期 6
·297-303
·
IF 0
Geng Chang-An,Chen Ji-Jun
Swerilactones H–K ( 1 – 4 ) as four unprecedented secoiridoid trimers represent a new type of natural product, which has attracted much interest of natural chemists due to their novel skeletons and promising bioactivity. In order to well understand their MS fragmentation behaviors, they were investigated by electrospray ionization ion-trap time-of-flight multistage product ion mass spectrometry (ESI-IT-TOF-MS n ) for the first time. The protonated molecules ([M+H] + ) of swerilactones J and K, and deprotonated molecules ([M−H] − ) of swerilactones H, J and K were readily observed in the conventional single-stage mass spectra (MS); however only the [M+Cl] − ion for swerilactone I was obtained in negative mode. Based on the MS n study, the fragmentation pathways of swerilactones H and I in negative mode, and swerilactones J and K in both positive and negative modes were proposed. The neutral losses of H 2 O, CO, CO 2 and C 2 H 4 O moieties are the particular elimination from the precursor ions due to the presence of hydroxyl, δ -lactone and 1- O -ethyl moieties in their structures, of which the retro-Diels–Alder cleavage was the most particular dissociation. The fragment ions at m/z 341 and 291 in negative mode can be considered as the diagnostic ions for secoiridoid trimers. This investigation will provide valuable information for their fast characterization from complicated natural mixtures and extensive understanding their structural architectures.
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PubMed
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2016
·Vol. 182
·期 1
·55-66
·
IF 1.606
Fu Ruijie,Yao Kai,Zhang Qisheng,Jia Dongying,Zhao Jiayuan,Chi Yuanlong
A series of collagen hydrolysates (CHs) were prepared from pigskin shavings by using pepsin (PCH), trypsin (TCH), Alcalase (ACH), HCl (HCH), and NaOH (NCH). Their physicochemical properties, including degree of collagen hydrolysis, molecular weight distribution, electric charge, and microstructure, were investigated, and their flocculation performance was evaluated in a kaolin suspension, at varied pHs and concentrations. PCH exhibited high flocculation capability under acidic and neutral conditions, and its efficiency for removing suspended particles was approximately 80% at a concentration of 0.05 g/L. TCH, ACH, HCH, and NCH showed almost no flocculation capability. The flocculation capability of PCH could be mainly due to a combination of optimal molecular weight distribution and electric charge. This study could provide an environment-friendly natural flocculant and also proposes a promising approach for the reuse of collagen wastes.
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高影响因子
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2016
·Vol. 129
·期 2
·767-775
·
IF 1.781
Luo Fubin,Wu Kun,Lu Mangeng,Yang Liu,Shi Jun
Aluminum hypophosphite (AHP) is surface modified by melamine derivative to fabricate reactive solid flame retardant (MCAHP) for polyurethane foam. MCAHP is successfully prepared and characterized by FTIR and SEM. The flame-retarded efficiency of MCAHP in PU is higher than that of AHP. It demonstrated that MCAHP has better compatibility in PU matrix compared with AHP based on the SEM observation. After surface modification, due to the reaction between MCAHP and PU matrix, crosslinking might be formed between MCAHP and PU matrix, which contributes to the excellent compatibility of MCAHP in PU matrix, and as a result, the glass transition temperature of PU/MCAHP is 4 °C higher than that of PU/AHP. The thermal behavior of PU composites is characterized by TG and TG-FTIR, and results suggest the sublimation of melamine at about 320 °C because of the decomposition of the melamine derivative. The sublimation of melamine can consume abundant heat and dilute the oxygen concentration, which is benefit for the improvement of flame retardancy.
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MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 12
·3293-3301
·
IF 4.831
Liu Lingyu,Ma Zhen,Zhu Xiaohua,Alshahrani Lina Abdullah,Tie Shaolong,Nan Junmin
A glassy carbon electrode was modified with carbon nanofragments and bismuth oxide, and the resulting electrode (CNF-Bi/GCE) was applied to the voltammetric determination of catechol (CC) in the presence of a large excess (>100-fold) of hydroquinone (HQ). The CNF-Bi was synthesized by anchoring bismuth oxide on CNFs in a hydrothermal and calcining process. Compared to CNF-modified GCE and bare GCE, the CNF-Bi/GCE exhibits enhanced electrocatalytic activity with respect to the redox reaction of CC (at 0.28 V vs. SCE) and HQ (at 0.15 V). Differential pulse voltammetry was applied to quantify traces of CC in the presence of excess HQ. The calibration plot is linear in the 3 to 20 μmol L −1 concentration range, with a 0.2 μmol L −1 detection limit (at a signal-to-noise ratio of 3). These results indicate the CNF-Bi/GCE is a promising sensor for analyzing trace isomers of dihydroxybenzene. In addition, it is also applicable to the determination of ascorbic acid, dopamine, resorcin, and rutin.
A glassy carbon electrode was modified with carbon nano-fragments and bismuth oxide (CNF-Bi/GCE). It was applied to the determination of trace levels of catechol (CC) in the presence of an up to >100 fold excess of hydroquinone (HQ).
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JOURNAL OF ELECTRONIC MATERIALS
·
2016
·Vol. 46
·期 2
·982-991
·
IF 1.491
Guo Huilong,Lu Mangeng,Liang Liyan,Wu Kun,Ma Dong,Xue Wei
In this work, liquid crystalline epoxies with lateral substituents were synthesized and cured with aromatic amines or anhydride. The liquid crystalline phase structure of liquid crystalline epoxies with lateral substituents was determined by polarized optical microscopy. The relationship between thermal conductivity and dielectric properties and liquid crystalline domain structure was discussed in the paper. The samples show high thermal conductivity up to 0.29 W/(m × K), due to the orientation of mesogenic units in epoxies. The sample's low dielectric constant of 2.29 is associated with the oriented mesogenic units and long nonpolar lateral substituents. This indicates a new way to obtain materials with high thermal conductivity and a low dielectric constant by introducing oriented mesogenic units into cross-linked epoxy systems. The water repellency is reflected in the contact angles of 92–98°, which are apparently higher than that of conventional epoxy systems. It was also found that the better toughness of liquid crystalline epoxies with lateral substituents was attributed to the existence of long flexible alkyl lateral substituents.
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高影响因子
Nature Protocols
·
2024
·
IF 13.1
Zhang Kailin,Shi Xiang,Jiang Haibo,Zeng Kaiwen,Zhou Zihao,Zhai Peng,Zhang Lihua,Peng Huisheng
Mono-dimensional fiber-based electronics can effectively address the growing demand for improved wearable electronic devices because of their exceptional flexibility and stretchability. For practical applications, functional fiber electronic devices need to be integrated into more powerful and versatile systems to execute complex tasks that cannot be completed by single-fiber devices. Existing techniques, such as printing and sintering, reduce the flexibility and cause low connection strength of fiber-based electronic devices because of the high curvature of the fiber. Here, we outline a twisting fabrication process for fiber electrodes, which can be woven into functional threads and integrated within textiles. The design of the twisted thread structure for fiber devices ensures stable interfacing and good flexibility, while the textile structure features easily accessible, interlaced points for efficient circuit connections. Electronic textiles can be customized to act as displays, health monitors and power sources. We detail three main fabrication sections, including the fabrication of the fiber electrodes, their twisting into electronic threads and their assembly into functional textile-based devices. The procedures require ~10 d and are easily reproducible by researchers with expertise in fabricating energy and electronic devices.
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高影响因子
CELLULOSE
·
2024
·Vol. 31
·期 6
·3947-3960
·
IF 4.9
Dong Chenshan,Yao Yiting,Li Sanchuan,Ma Dongdong,Zhang Ruquan,Liu Xin,Shang Bin,Huang Jingjing,Gu Shaojin,Du Jiehao
The application of functional textiles in our daily life is expanding increasingly. However, manufacturing, designing, and developing mild but efficient functional textiles remains a challenge. In this work, a straightforward method to construct functional cotton fabrics (CF) with superhydrophobicity and antibacterial activity using dipentaerythritol pentaacrylate-branched poly(ethyleneimine) (5Acl-BPEI) reactive coating as the secondary reactions layer and subsequently treating with bis(3-aminopropyl)-terminated poly(dimethylsiloxane) (PDMS-NH 2 ) and 1-(12-(mercaptododecyl)-3-methylimidazolium bromide (MDMIBr). The obtained CF@5Acl-BPEI@PDMS-NH 2 showed integrated performances with hydrophobic behavior (water contact angle of 151.7 ± 0.8° and sliding angle of 8.8 ± 0.9°), self-cleaning, excellent efficiency in oil–water separation (above 99.4%). Additionally, the oil flux remained consistently high, exceeding 4000 L m −2 h −1 after 10 cycles in various oil–water mixtures and it remained stable under various harsh conditions. And the prepared CF@5Acl-BPEI@MDMIBr significantly enhances the antibacterial properties of textiles, exhibiting a bacteriostatic rate of 99%. In addition, the air permeability, whiteness and thermal stability of CF after coating and surface modification have no obvious changes. These results indicate that the 5Acl-BPEI coating serves as a versatile platform for the development of various functional materials, providing invaluable insights into coating modification.
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JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2024
·Vol. 39
·期 4
·962-967
·
IF 1.3
Liu Zhigang,Tang Zezheng,Yang Lirong,Wang Chunmei,Xie Yuantao,Liu Yisen
The retarding effect of protein retarder on phosphorus building gypsum (PBG) and desulfurization building gypsum (DBG) was investigated, and the results show that protein retarder for DBG can effectively prolong the setting time and displays a better retarding effect, but for PBG shows a poor retarding effect. Furthermore, the deterioration reason of the retarding effect of protein retarder on PBG was investigated by measuring the pH value and the retarder concentration of the liquid phase from vacuum filtration of PBG slurry at different hydration time, and the measure to improve the retarding effect of protein retarding on PBG was suggested. The pH value of PBG slurry (<5.0) is lower than that of DBG slurry (7.8–8.5). After hydration for 5 min, the concentration of retarder in liquid phase of DBG slurry gradually decreases, but in liquid phase of PBG slurry continually increases, which results in the worse retarding effect of protein retarder on PBG. The liquid phase pH value of PBG slurry can be adjusted higher by sodium silicate, which is beneficial to improvement in the retarding effect of the retarder. By adding 1.0% of sodium silicate, the initial setting time of PBG was efficiently prolonged from 17 to 210 min, but little effect on the absolute dry flexural strength was observed.
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IONICS
·
2024
·
IF 2.4
Duan Jinrong,Si Fang,Wang Jiafen,Lu Linlin,Liu Jia,Song Yanyan,Mo Jiahui,Yu Jincui,Gao Jinlong
Fuel cell is considered the best candidate power source because of its high energy conversion rate, large capacity, and zero emission. However, noble metal catalysts as a key core material have some problems, such as high cost and poor durability. Therefore, in this paper, the active self-supporting nanoporous Pd–Ag catalysts are successfully formed by cryogenic treatment and one-step dealloying treatment of Al-Pd–Ag ribbons. The surface of the catalyst ligaments shows a certain degree of preferred orientation for high-index facets (220) and (311). The results show that the activity and stability of the catalysts for methanol electrocatalytic oxidation are significantly improved under the synergistic effect of special configuration and promoter Ag. The electrocatalytic activity of the catalysts is about 9.7 and 15.2 times that of commercial Pt/C and commercial Pd/C catalysts, respectively. After 5000 s, the current density of the 1800 rpm-deep2 sample is 382.88 mA·mg −1 , which is about 14.1 times and 39.9 times that of commercial Pt/C catalyst and commercial Pd/C catalyst.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2024
·Vol. 31
·期 39
·51673-51686
·
IF 0
Wang Xi,Hou Fali,Zhai Jinli,Yan Jiali,Gong Chao,Li Yinghao,Zhu Wenjie,Luo Yongming,Yang Daoli,Gao Xiaoya
Considering the high cost and complicated recycling process of spent lithium-ion batteries (SLIBs), transforming SLIBs into environment functional materials may be a wise approach. Herein, lithium cobaltite (LCO) cathode powders recovered from SLIBs were used to activate peroxymonosulfate (PMS) for removing carbamazepine (CBZ). The recovered LCO enables a 98.2% removal efficiency of CBZ (2.5 mg/L) within 10 min, which was effective at a broader pH range (pH = 5.0–11.0). The influence of key factors (initial pH, PMS, and catalyst dosage) and coexisting substances (SO 4 2− , H 2 PO 4 − , NO 3 − , Cl − , HCO 3 − , and HA) on CBZ degradation were examined in detail. The primary radical species during the degradation of CBZ were proved to be 1 O 2 , SO 4 − , and . OH that generated from PMS activation initiated by the valence change of Co in recovered LCO. The recovered LCO displayed excellent reusability with about 80.0% removal of CBZ after six cycles. Homogeneous activation of PMS mainly contributed to CBZ degradation in the first run, but the recovered LCO catalyst dominated the heterogeneous activation of PMS for the degradation of CBZ in the second to sixth run. Finally, the CBZ degradation pathways were presented based on the identified intermediates. This research has offered a new strategy of “treating wastes with wastes” to maximize the recycling of electronic wastes to remove emerging pollutants.
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Journal of Superconductivity and Novel Magnetism
·
2024
·
IF 1.6
Pi Siwen,Li Jie,Zhang Yingde,Zhou Weina,Shan Shuo,Zhao Xuan
Ce-substituted M-type Sr 1– x Ce x Fe 12 O 19 ( x = 0.00–0.08) ferrites were successfully synthesized via the ball milling method, and the sintering temperature at 1200 °C for 2 h. The synthesized materials were characterized by X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), and vibrating sample magnetometry (VSM). The elemental makeup of the samples was characterized by energy-dispersive spectrometry (EDS). The XRD results showed the crystal structure and purity of the samples. The FTIR spectroscopy indicated tetrahedral and octahedral tensile vibrations of M-type strontium ferrite at two characteristic peak bands of 554 and 454 cm −1 . The FESEM showed that Ce doping resulted in fine grains. The EDS indicated that Ce content increased with decreasing Sr concentration but that the concentrations of other elements remained basically unchanged. The mass ratio of Sr:Fe is calculated from EDS results and the measured Sr:Fe mass ratio is quite close to the mass ratio calculated from the reactants used to synthesize each sample. The VSM showed that at a Ce doping of x = 0.02, the coercivity of the samples reached the maximum, and the coercivity values and the saturation magnetization were 3878.25 Oe and 73.625 emu/g, respectively. And such high thermal stability further determines that Ce-substituted strontium ferrites have infinite potential in application fields (T = 450.2 °C).
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