Nanomaterials
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2022
·Vol. 12
·期 17
·2933
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IF 5.719
Hongyu Lv,Meng Zhao,Yiran Li,Kun Li,Shaojuan Chen,Wenwen Zhao,Shaohua Wu,Yantao Han
The design and development of novel dressing materials are urgently required for the treatment of chronic wounds caused by diabetic ulcers in clinics. In this study, ursolic acid (UA) extracted from Chinese herbal plants was encapsulated into electrospun nanofibers made from a blend of chitosan (CS) and polyvinyl alcohol (PVA) to generate innovative CS-PVA-UA dressings for diabetic wound treatment. The as-prepared CS-PVA-UA nanofiber mats exhibited randomly aligned fiber morphology with the mean fiber diameters in the range of 100–200 nm, possessing great morphological resemblance to the collagen fibrils which exist in the native skin extracellular matrix (ECM). In addition, the CS-PVA-UA nanofiber mats were found to possess good surface hydrophilicity and wettability, and sustained UA release behavior. The in vitro biological tests showed that the high concentration of UA could lead to slight cytotoxicity. It was also found that the CS-PVA-UA nanofiber dressings could significantly reduce the M1 phenotypic transition of macrophages that was even stimulated by lipopolysaccharide (LPS) and could effectively restore the M2 polarization of macrophages to shorten the inflammatory period. Moreover, the appropriate introduction of UA into CS-PVA nanofibers decreased the release levels of TNF-α and IL-6 inflammatory factors, and suppressed oxidative stress responses by reducing the generation of reactive oxygen species (ROS) as well. The results from mouse hepatic hemorrhage displayed that CS-PVA-UA nanofiber dressing possessed excellent hemostatic performance. The in vivo animal experiments displayed that the CS-PVA-UA nanofiber dressing could improve the closure rate, and also promote the revascularization and re-epithelization, as well as the deposition and remodeling of collagen matrix and the regeneration of hair follicles for diabetic wounds. Specifically, the mean contraction rate of diabetic wounds using CS-PVA-UA nanofiber dressing could reach 99.8% after 18 days of treatment. In summary, our present study offers a promising nanofibrous dressing candidate with multiple biological functions, including anti-inflammation, antioxidation, pro-angiogenesis, and hemostasis functions, for the treatment of hard-to-heal diabetic wounds.Keywords:electrospinning;bioactive nanofibers;ursolic acid;wound dressing;diabetic wound
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CARBOHYDRATE POLYMERS
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2020
·
IF 7.182
Jian He,Xulin Hu,Jianfei Cao,Yu Zhang,Jianping Xiao,liJun Peng,Dongliang Chen,Chengdong Xiong,Lifang Zhang
Biocompatible polymers and drug-delivery scaffolds have driven development in bone regeneration. In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility. The incorporation of PLLA , OA-HA, and VH into PTMC microspheres not only slowed the biodegradability of the scaffold but also enhanced its mechanical properties and surface properties. Moreover, the CS coating stimulated extensive adhesion of osteoblasts before OA-HA incorporation, which facilitated the controlled release of OA-HA. The scaffolds were characterized via scanning electron microscopy, in vitro comprehensive performance testing, cell culturing, and microcomputer tomography scanning. The results indicated that the surface of the composite microsphere scaffold was suitable for osteoblast adhesion. Additionally, the release of OA-HA stimulated osteogenic proliferation. Our findings suggest that the CS-PTMC/PLLA/OA-HA/VH microsphere scaffold is promising for bone tissue engineering applications.
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ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Bin Cai,Qin Zou,Yi Zuo,Quanjing Mei,Jinqi Ma,Lili Lin,Li Chen,Yubao Li
There is increasing demand for biomaterials that both assist with bone regeneration and have anti-infection qualities in clinical applications. To achieve this goal, chitosan microspheres with either positive or negative charges were fabricated and then assembled as a gel for bone healing. The positively charged chitosan microspheres (CSM; ∼35.5 μm) and negatively charged O-carboxymethyl chitosan microspheres (CMCSM; ∼13.5 μm) were loaded, respectively, with bone morphogenetic protein (BMP-2) and berberine (Bbr) via swollen encapsulation and physical adsorption without a significant change in the electric charges. The release kinetics of BMP-2 and Bbr from the microspheres were also studied in vitro. The results showed that the Bbr/CMCSM microsphere group possessed high antibacterial activity against Staphylococcus aureus; the BMP-2/CSM microsphere group also had excellent cytocompatibility and improved osteoinductivity with the assistance of BMP-2. The assembled gel group consisting of Bbr/CMCSM and BMP-2/CSM had a porous structure that allowed biological signal transfer and tissue infiltration and exhibited significantly enhanced bone reconstruction compared with that of the respective microsphere groups, which should result from the osteoconductivity of the porous structure and the osteoinduction of the BMP-2 growth factor. The oppositely charged microspheres and their assembled gel provide a promising prospect for making injectable tissue-engineered constructs with regenerative and anti-infective dual effects for biomedical applications.
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MATERIALS LETTERS
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2012
·
IF 2.307
Chang Gao,Yurong Cai,Xiangdong Kong,Guoxiang Han,Juming Yao
The injectable chitosan/β-glycerophosphate (CS/GP) hydrogels were prepared with dexamethasone (Dex)/recombinant human bone morphogenetic protein (rhBMP-2) loaded hydroxyapatite nanoparticles (nHAP). The obtained samples were characterized by TEM, SEM, XRD and nitrogen adsorption tests, and the effect of Dex and rhBMP-2 on the mesenchymal stem cells (MSCs) was evaluated. The results showed that nHAP could be dispersed homogeneously in the matrix of injectable CS/GP hydrogels and the combination of Dex and rhBMP-2 could promote the proliferation and osteoblastic differentiation of MSCs, indicating that the prepared injectable CS/GP hydrogels with Dex and rhBMP-2 loaded nHAP have great potential in different orthopedic regenerative applications.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2021
·
IF 5.875
Yu Tao,Ding Qu,Chunli Tian,Yingshuang Huang,Lingjing Xue,Caoyun Ju,Meixi Hao,Can Zhang
Amphiphilic chitosan derivatives have attracted wide attention as drug carriers due to their physicochemical properties. However, obtaining a desired amphiphilic chitosan derivative by tuning the various functional groups was complex and time-consuming. Therefore, a facile and common synthesis strategy would be promising. In this study, a modular strategy based on strain-promoted azide-alkyne cycloaddition (SPAAC) click reaction was designed and applied in synthesizing deoxycholic acid- or octanoic acid-modified N -azido propionyl- N,O -sulfate chitosan through tuning the hydrophobic groups. Additionally, chitosan derivatives with the same substitute groups were prepared via amide coupling as controls. We demonstrated that these derivates via the two strategies showed no obvious difference in physicochemical properties, drug loading ability and biosafety, indicating the feasibility of modular strategy. Notably, the modular strategy exhibited advantages including high reactivity, flexibility and reproducibility. We believe that this modular strategy could provide varied chitosan derivatives in an easy and high-efficiency way for improving multifunctional drug carriers.
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Materials Science & Engineering C-Materials for Biological Applications
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2019
·
IF 4.959
Qingchang Chen,Jun Wu,Yuan Liu,Yanqun Li,Chuqiu Zhang,Weichen Qi,Kelvin W.K. Yeung,Tak Man Wong,Xiaoli Zhao,Haobo Pan
Cutaneous wounds, especially chronic wounds, remain clinical challenges, and this is partially due to the complex healing process composed of four overlapping but distinct stages including hemostasis, inflammation, proliferation and remodeling. Therefore, wound dressings with spatially designed structures which can temporally regulate certain bioactive components to function at specific healing stages might be able to accelerate the healing process. In this study, nanobioglass incorporated chitosan-PVA (polyvinyl alcohol) trilayer nanofibrous membrane (nBG-TFM) was fabricated via sequential electrospinning. This membrane exhibited excellent biocompatibility, antibacterial activity and regeneration promotion effect. Furthermore, spatially designed structure optimized functions of each component and provided more suitable microenvironment as compared with uniform membrane. Rat full-thickness skin defects model and mice diabetic chronic wound model showed that nBG-TFM could achieve significantly accelerated and enhanced healing, in terms of complete re-epithelialization, improved collagen alignment and formation of skin appendages. It was revealed that nBG-TFM functioned through upregulating growth factors including VEGF and TGF-β. Meanwhile inflammatory cytokines such as TNF-α and IL-1β were downregulated. The technology presented in this study shed new light on designing functional wound dressings which can promote healing of chronic wounds.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
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IF 5.518
Shiqing Li,Wenhui Shi,Xiaoyan Wang,Xiaona Hu,Shujing Li,Yuxi Zhang
In this study, a multifunctional food packaging material (G-Eug-NPs-NF) based on gelatin nanofiber membranes (G-NF) containing eugenol nanoparticles (Eug-NPs) were prepared. The Eug-NPs were fabricated through the electrostatic self-assembly by using two ionic polysaccharides chitosan and sulfobutylether-β-cyclodextrin (SCD). Compared with G-NF, the G-Eug-NPs-NF exhibited better antioxidant capacity , which positively correlated with Eug-IC concentration, up to 84.17%. In addition, the latter had a good inhibitory effect on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) , and was more conducive to inhibit the growth of S. aureus . At the same time, the sustained release experiment showed that the incorporation of Eug-NPs in nanofiber membranes improved the sustained release ability of G-Eug-NPs-NF. G-Eug-NPs-NF exhibits the promising application of Eug in food, medicine and other fields.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Huimei Shan,Sanxi Peng,Chaoran Zhao,Hongbin Zhan,Chunya Zeng
A goethite/graphene oxide/chitosan (α-FeO(OH)/GO/CS) nanocomposite adsorbent was prepared and firstly used to remove As(III) from aqueous solution. The composite was characterized by FTIR , XPS, XRD , and EDS techniques. Batch experiments were conducted to investigate the effects of several factors (initial concentration, pH, m/v, contact time, co-existing ions, and temperature) on As(III) adsorption and to evaluate adsorption kinetic , equilibrium isotherm, and thermodynamics. Results showed that As(III) adsorption increased with the increasing initial concentration, contact time, and temperature, but decreased with the increasing m/v and co-existing ions concentrations of SO 4 2− , PO 4 3− and Fe 3+ . As(III) adsorption remained high at a wide pH range of 3–10. The adsorption was well fitted to a pseudo-second-order kinetic model and was endothermic and spontaneous. The best fit of As(III) adsorption with the Freundlich and Sips models indicated that it was monolayer adsorption, and the maximum adsorption capacity was 289.42 mg/g. As(III) removal was related to -NHCO-, C O, O H, and Fe O groups, but the complexation between As(III) ions and hydroxyl iron oxide was the major contributor. After the fifth desorption, the removal efficiency was still as high as 79.6%, indicating excellent reusability. Thus, this composite had great potential for removing As(III) from aqueous solutions.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2022
·
IF 5.062
Jie Zhang,Zetao Wang,Muheng Liao,Shuxian Li,Qi Feng,Xiaodong Cao
Transdermal administration can avoid the first-pass effect of liver, improve the bioavailability of drugs and the adaptability of patients. However, there are still many problems, such as low load efficiency, short drug half-life, unfavorable transdermal efficiency, poor skin compatibility, which hinder the development of transdermal administration. In order to solve the above problems, amphiphilic chitosan microemulsion (AC-ME) is developed by using chitosan with hydrophilic carboxyl groups and hydrophobic long alkane chains as surfactant. AC-ME is efficient for drug loading and transdermal delivery , which is demonstrated by extremely high drug loading, improved drug stability and excellent transdermal efficiency. Specifically, the loading amount of curcumin (Cur) can reach up to 3.41 ± 0.13 mg/mL, an increase of approximately 30,000 times compared to its water solution. The light stability of Cur is improved from 43% to 75%. The transdermal process and skin retention of Cur exhibit 1.76-fold and 5.29-fold increase, respectively. Moreover, AC-ME is skin-compatible and does not damage skin structure. Finally, AC-ME can improve the therapeutic effect of Cur and exhibit superb anti-inflammatory effect in the rheumatoid arthritis (RA) model of mice. In a word, AC-ME would be a promising drug carrier for transdermal drug delivery.
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CELL TRANSPLANTATION
·
2017
·Vol. 26
·期 8
·1331-1340
·
IF 3.006
Xionglin Chen,Min Zhang,Shixuan Chen,Xueer Wang,Zhihui Tian,Yinghua Chen,Pengcheng Xu,Lei Zhang,Lu Zhang,Lin Zhang
Skin wound healing is a complicated process that involves a variety of cells and cytokines. Fibroblasts play an important role in this process and participate in transformation into myofibroblasts, the synthesis of extracellular matrix (ECM) and fibers, and the secretion of a variety of growth factors. This study assessed the effects of peptide Ser-Ile-Lys-Val-Ala-Val (SIKVAV)--modified chitosan hydrogels on skin wound healing. We investigated the capability of peptide SIKVAV to promote cell proliferation and migration, the synthesis of collagen, and the secretion of a variety of growth factors using fibroblasts in vitro. We also treated skin wounds established in mice using peptide SIKVAV-modified chitosan hydrogels. Hematoxylin and eosin staining showed that peptide-modified chitosan hydrogels enhanced the reepithelialization of wounds compared with negative and positive controls. Masson's trichrome staining demonstrated that more collagen fibers were deposited in the wounds treated with peptide-modified chitosan hydrogels compared with the negative and positive controls. Immunohistochemistry revealed that the peptide-modified chitosan hydrogels promoted angiogenesis in the skin wound. Taken together, these results suggest that peptide SIKVAV-modified chitosan hydrogels may be useful in wound dressing and the treatment of skin wounds.
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Materials Science & Engineering C-Materials for Biological Applications
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2016
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IF 3.42
Huinan Chen,Xiaodong Xing,Huaping Tan,Yang Jia,Tianle Zhou,Yong Chen,Zhonghua Ling,Xiaohong Hu
An antibacterial and biodegradable composite hydrogel dressing integrated with microspheres is developed for drug delivery and wound healing. The mechanism of gelation is attributed to the Schiff-base reaction between aldehyde and amino groups of oxidized alginate (OAlg) and carboxymethyl chitosan (CMCS). To enhance antibacterial and mechanical properties, tetracycline hydrochloride (TH) loaded gelatin microspheres (GMs) were fabricated by an emulsion cross-linking method, followed by integrating into the OAlg-CMCS hydrogel to produce a composite gel dressing. In vitro gelation time, swelling, degradation, compressive modulus and rheological properties of the gel dressing were investigated as the function of microsphere ratios. With increasing ratios of microspheres from 10 to 40 mg/mL, the composite dressing manifested shorter gelation time and lower swelling ratios, as well as higher mechanical strength. Comparing to other formulations, the gel dressing with 30 mg/mL microspheres showed more suitable stabilities and mechanical properties for wound healing. Also, in vitro drug release results showed that the loaded TH could be sustained release from the composite gel dressing by contrast with pure hydrogels and microspheres. Furthermore, powerful bacteria growth inhibition effects against Escherichia coli and Staphylococcus aureus suggested that the composite gel dressing, especially the one with 30 mg/mL GMs containing TH, has a promising future in treatment of bacterial infection.
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CARBOHYDRATE POLYMERS
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2021
·
IF 9.381
Tao Liu,Shaohua Gou,Yang He,Shenwen Fang,Lihua Zhou,Guangjun Gou,Lang Liu
In this paper, N -methylene phosphonic acid chitosan (NPCS-PEI) was synthesized from chitosan, phosphorous acid , formaldehyde and hyperbranched polyethyleneimine (PEI), and Cu 2+ and Pb 2+ removal performance was examined in aqueous solution. NPCS-PEI exhibited three-dimensional porous architectures, with a specific surface area of 490.61 m 2 /g. The effects of pH, initial concentration, adsorption time, temperature and ionic strength on the adsorption capacity were investigated. The adsorption kinetics indicated that Cu 2+ and Pb 2+ adsorption onto NPCS-PEI follows a pseudo-second-order model. The adsorption isotherms agree well with the Langmuir isotherm model, and the maximum adsorption capacities of Cu 2+ and Pb 2+ on the NPCS-PEI are approximately 276.12 and 645.16 mg/g, respectively. The adsorption efficiency of NPCS-PEI remained above 85% after 5 adsorption-desorption successive cycles. Moreover, the NPCS-PEI aerogels had selective adsorption toward Cu 2+ . The FTIR and XPS analysis proved that amino, hydroxyl, and phosphonic acid groups were involved in the chelation with metal ions .
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