ELECTROCHIMICA ACTA
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2017
·
IF 4.798
Fengxia Zhang,Ping Zhang,Qiong Wu,Wenjing Xiong,Qi Kang,Dazhong Shen
Herein, we proposed a simple method to measure the inner resistance of a photoelectrochemical (PEC) sensor. Three photoanodes, including TiO 2 nanotubes array (TiO 2 NTs), g-C 3 N 4 /TiO 2 NTs and Mn 3 (BTC) 2 /g-C 3 N 4 /TiO 2 NTs (BTC = 1,3,5-benzene- tricarboxylic acid), were chosen as the models. It was shown the change in inner resistance of the photoanode is the main factor for the photocurrent response in a PEC sensor. Under light excitation, the inner resistance of the photoanode is reduced remarkably, resulting in the increment in the photocurrent. The influence of the light intensity on the inner resistance is less than that on the photocurrent, due to the synchronous change in photovoltage . With increasing concentration of hole scavengers (e.g., H 2 O 2 , ascorbic acid), the steady inner resistances of the three photoanodes are decreased. In the PEC sensor with Mn 3 (BTC) 2 /g-C 3 N 4 /TiO 2 NTs, the Mn 3 (BTC) 2 layer is served as a size-exclusion filter to prevent the diffusion of molecules with size larger than the apertures in crystals, which improves the selectivity of the PEC sensor to smaller molecules (e.g., H 2 O 2 ). The catalytic effect of Mn 3 (BTC) 2 to H 2 O 2 enhances the PEC response sensitivity to H 2 O 2 . Under optimized conditions, the reciprocal of the averaged steady effective inner resistance of Mn 3 (BTC) 2 /g-C 3 N 4 /TiO 2 NTs is in a linear correlation with the logarithmic concentration of H 2 O 2 in the range of 0.003 to 10 μM with a limit of detection of 1 nM. This PEC sensor was applied for H 2 O 2 determination in exhaled breath condensate samples.
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APPLIED CATALYSIS B-ENVIRONMENTAL
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2016
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IF 8.328
Xia Chen,Wangyang Lu,Tiefeng Xu,Nan Li,Dandan Qin,Zhexin Zhu,Gangqiang Wang,Wenxing Chen
In nature, metalloporphyrins, such as chlorophyll, cytochrome P450 and so forth, are key materials in maintaining the ecological cycles, especially the carbon cycle, and play an important role in both photosynthesis and the catalytic oxidation of organisms. Inspired by these factors, we skillfully combined photocatalysis and biomimetic catalysis using imidazole (IMD)-functionalized modification of g-C 3 N 4 and axial coordination with hemin. Compared with pure hemin, g-C 3 N 4 and a mixture of the two, our novel catalytic system (g-C 3 N 4 -IMD-hemin/H 2 O 2 ) showed high photocatalytic oxidation activity for the degradation of 4-chlorophenol (4-CP), and the stability of hemin was enhanced under solar irradiation. Furthermore, the effect of pH and the sustained photocatalytic oxidation stability of g-C 3 N 4 -IMD-hemin for degrading 4-CP were investigated. The results indicated that g-C 3 N 4 -IMD-hemin presents a high photocatalytic oxidation activity over a wide pH range and exhibits good recyclability. A series of designed experiments showed that superoxide radicals ( O 2 − ), high-valent iron (Fe(IV) = O) species, peroxy radicals ( OOH) and few hydroxyl radicals ( OH) were generated in the g-C 3 N 4 -IMD-hemin/H 2 O 2 system. This synergistic photocatalytic and biomimetic process offers new insight for the utilization of solar energy and offers a new perspective for the exploration of catalysts for environmental remediation.
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RSC Advances
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2017
·Vol. 7
·期 40
·24628-24632
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IF 3.108
Jiaona Xu,Chunying Wei
A novel, label-free fluorescence detection method was proposed for the formation of the G-quadruplex DNAs based on placing two DNA-Ag NCs together to light-up fluorescence. Herein, we designed two tailored DNA sequences noted as target DNA (abbreviated as T-DNA) and complementary DNA (abbreviated as C-DNA), respectively. T-DNA contains two cytosine-rich sequences at the 3′- and 5′-end responsible for Ag NCs synthesis, together with the guanine-rich (G-rich) DNA sequence in the middle segment. C-DNA was designed completely complementary to the G-rich segment of the T-DNA. Provided that the middle G-rich oligonucleotide of T-DNA folds into G-quadruplex in the presence of monovalent cations, the located at 3′ and 5′ terminuses two darkish DNA-Ag NCs approximate, leading to the enhanced fluorescence signal readout. Upon addition of the C-DNA, the G-quadruplex structure is unfolded and the two DNA-Ag NCs are separated, then the fluorescence signal is quenched. On the contrary, when the G-rich oligonucleotide with mutational bases failed to form the G-quadruplex, the fluorescence signal decreased clearly. This design allowed specific and general detection of the formation of diverse G-quadruplexes with easy operation and low cost. We believed that the proposed approach laid a foundation for the researchers in bioimaging, pharmacology, diagnostics, and other fields.
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Analytical Methods
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2015
·Vol. 7
·期 21
·9340-9346
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IF 1.821
Kewei Wang,Na Sun,Xinpan Li,Ruihua Zhang,Guangyue Zu,Jine Wang,Renjun Pei
This study reported the development of a p–n heterojunction photoelectrochemical biosensor using a G-quadruplex as the recognition system. The n-type ZnO nanorods and p-type BiOI nanoflakes were combined to form the heterostructure by in situ growth of BiOI attributed to a simple impregnating-hydroxylation method. This simple p–n heterojunction fabrication method could facilitate the development of rapid and sensitive visible light activated PEC sensors. In this study, the ZnO/BiOI photoelectrode showed a rapid photocurrent decrease in the presence of Pb2+ due to the electron withdrawing effect of hemin which bound with the G-quadruplex/Pb2+ complex. This PEC biosensor exhibited a good response following the concentration of Pb2+ in the range from 10 to 100 μM.
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高影响因子
ACS Sustainable Chemistry & Engineering
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2014
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IF 0
Dong J. Wang,Hou Chen,Hui Xu,Jin M. Sun,Yuan Y. Xu
Polyacrylonitrile (PAN) was grafted onto the surface of a wheat straw matrix (WSM) by single-electron transfer-living radical polymerization (SET-LRP). A novel agricultural residue adsorbent amidoxime WSM-g-PAN (AO WSM-g-PAN) was obtained by modifying WSM-g-PAN with hydroxylamine hydrochloride (NH2OH·HCl). AO WSM-g-PAN was used to remove heavy metal ions. Compared with WSM, the adsorption capacity of AO WSM-g-PAN for Hg(II) increased greatly, and the maximum adsorption capacity was 4.7 mmol g–1. AO WSM-g-PAN could effectively adsorb Hg(II) from binary ion systems in the existence of Ni(II), Pb(II), Cu(II), Zn(II), and Ag(I).
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RSC Advances
·
2014
·Vol. 4
·期 106
·61759-61766
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IF 3.708
Hongmei Chen,Xingrong Tan,Juanjuan Zhang,Qiyi Lu,Xin Ou,Yuan Ruo,Shihong Chen
In this article, a new electrochemiluminescent (ECL) biosensor based on a g-C3N4–hemin nanocomposite and hollow gold nanoparticles (HGNPs) was constructed to detect lactate. Firstly, the g-C3N4 nanosheets were prepared through ultrasonication-assisted liquid exfoliation of bulk g-C3N4, which was obtained through polymerizing melamine under 600 °C. Then, the nanocomposites of g-C3N4 nanosheets and hemin were prepared to modify a glassy carbon electrode. Subsequently, HGNPs were self-assembled onto the electrode for adsorbing lactate oxidase to achieve a lactate biosensor. Due to the excellent catalytic effect of g-C3N4–hemin and HGNPs on the luminol/H2O2 ECL system, the as-prepared biosensor exhibited a good response performance to lactate with a linear range of 1.7 × 10−8 to 5.0 × 10−4 M and a detection limit of 5.5 × 10−9 M. In addition, the prepared ECL biosensor exhibited satisfying reproducibility and stability. The g-C3N4–hemin nanocomposite might have great potential application in a luminol/H2O2 ECL system.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
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2015
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IF 2.96
Junhui Pan,Langhong Wang,Guowen Zhang,Deming Gong
The characteristics of binding of 2-tert-butyl-4-methylphenol (TBMP), a synthetic phenolic antioxidant with hydroxypropyl-β-cyclodextrin (Hp-βCD) and calf thymus DNA (ctDNA) were investigated by multi-spectroscopic techniques and molecular simulation. The results indicated that TBMP preferred to form a 1:1 inclusion complex with Hp-βCD, with an inclusion constant of determined to be 7.15 × 10 3 L mol −1 . The intercalative mode of TBMP to ctDNA was supported by ctDNA melting temperature and relative viscosity studies, salt quenching effect, competitive binding with methylene blue and molecular modeling. The changes in Fourier transformed infrared (FT-IR) and circular dichroism (CD) spectra suggested that TBMP mainly bound to the G–C rich region with inducing a significant perturbation in B-like DNA structure. It was also found that Hp-βCD decreased the binding ability of TBMP with ctDNA, but did not affect the interactive mode between TBMP and ctDNA, and the formed inclusion complex of TBMP–Hp-βCD decomposed in the presence of ctDNA. This study may provide insights into the mechanism of binding of TBMP with ctDNA and the role of Hp-βCD in the TBMP–ctDNA interaction.
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PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2015
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IF 6.409
Pei Zou,Yaling Liu,Hongyong Wang,Jun Wu,Feifan Zhu,Hao Wu
Detection of ultralow concentration of specific protein plays a key role in biotechnological applications and medical diagnostics. In this study, we demonstrate an amplified chemiluminescence biosensing platform for sensitive detection of protein. The biosensing platform ingeniously combines target-catalyzed hairpin assembly and Exo III-assisted signal amplification . A catalytic G-quadruplex-hemin DNAzyme is further employed to stimulate the generation of chemiluminescence in the presence of luminol and H 2 O 2 . These designs together allow a high sensitivity for the biotarget, human α-thrombin, resulting in a detection limit of 0.92 pM, and it is much lower than previous reported studies. In addition, the proposed biosensing platform is versatile. By conjugating with various recognition units, it could be employed to sensitive detect various DNA-binding proteins and might find wide applications in biomedical fields .
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PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2017
·
IF 6.035
Chen Li,Lei Wang,Xudong Wang,Mengxiao Kong,Quan Zhang,Guangyuan Li
Thermally induced graft copolymerization of sodium styrene sulfonate (SSS) with ozone-preactivated poly(vinylidene fluoride) (PVDF) produced a PVDF-g-PSSS copolymer . Sulfonic acid proton exchange membranes (PVDF-g-PSSA) were then prepared using the solvent evaporation method. They were investigated with a Fourier transform infrared spectrometer and an X-ray diffractometer. The results indicate that the proton conductivity of the prepared membranes reached 0.046 S/cm when the degree of grafting was 50.52%. Results from a quartz crystal microbalance with dissipation monitoring indicated that the PVDF-g-PSSA membrane showed superior antifouling performance to that of a Nafion membrane. The performance of the prepared membrane in dual-chamber microbial fuel cells was also evaluated and compared with that of Nafion 117. Although the microbial fuel cell with the prepared membrane generated lower maximum power density (106.7 mW/m 2 ) than that with Nafion 117 (132.0 mW/m 2 ), it was more cost-effective because of its lower price and more simplified preparation process. In addition, its chemical oxygen demand (COD) removal (85%) was much higher than that of Nafion 117 (74%).
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2017
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IF 5.401
Haiyan Wang,Pei Liu,Wenjing Jiang,Xue Li,Huanshun Yin,Shiyun Ai
DNA methylation is one of the important epigenetics events, and it is responsible for transcription, genomic imprinting and cellular differentiation. Aberrant DNA methylation is always contacted with various diseases. Herein, a simple and low-cost photoelectrochemical (PEC) biosensing method is proposed for detection of DNA methylation, assay of DNA methyltransferase (MTase) activity and screening of MTase inhibitor, which is based on M. SssI MTase- HpaII endonuclease system. The graphite-like C 3 N 4 (g-C 3 N 4 ) and CdS quantum dots (CdS QDs) are used as photoactive materials. After the double-stranded DNA (dsDNA) was treated with M. SssI MTase in the presence of S -adenosylmethionine, the methylated dsDNA cannot be digested by HpaII endonuclease and dithiol group at the terminal of dsDNA can be retained. As a result, CdS QDs could be coupled successfully with methylated dsDNA between the reaction of CdS QDs with dithiol of dsDNA, achieving the amplification of PEC response. Thus, the photocurrent of PEC biosensor is consistent with the M. SssI activity. The increased photocurrent was in proportion to M. SssI activity in the linear range from 1 to 80 U/mL with a detection limit of 0.316 U/mL. Furthermore, the effects of atrazine and azamethipos on M. SssI MTase activity were investigated, which might provide useful information on carcinogenic mechanism of pesticide. The PEC biosensor has potential to screen of DNA MTase inhibitor, providing valuable information for anti-cancer drug research and also for cancer therapy.
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高影响因子
Green Energy & Environment
·
2025
·
IF 14.6
Jinshan Chen,Jiangfeng Lu,Ran Lang,Chi Wang,Shuangyou Bao,Yuan Li,Kai Li,Maohong Fan
In this study, a novel Pt-loaded CuPc/g-C 3 N 4 (PtCuCN) composite was synthesized for the selective photocatalytic reduction of CO 2 to CH 4 under visible light. The PtCuCN catalyst achieved a CH 4 yield of 39.8 μmol g⁻ 1 h⁻ 1 , significantly outperforming bulk g-C₃N₄ and CuPc alone by factors of 2.5 and 3.1, respectively, with a high selectivity of 90%. In comparison with other commonly studied photocatalysts, such as g-C 3 N 4 -based catalysts, the PtCuCN composite exhibited superior CH 4 yield and product selectivity, demonstrating its potential as a more efficient photocatalyst for CO₂ reduction. X-ray photoelectron spectroscopy (XPS), density functional theory (DFT) calculations, and in-situ infrared (IR) analysis revealed that the Pt⁰ species effectively lower the activation energy for CH 4 formation, while CuPc extends the light absorption range and enhances charge separation. The combined effects of these components in a Z-scheme heterojunction provide new insights into designing highly selective CO 2 -to-CH 4 photocatalysts. This work demonstrates the potential of PtCuCN as a highly efficient and stable catalyst for CO 2 reduction to CH 4 under visible light.
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Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Decai Yang,Qing Ye,Chao Qu,Fanwei Meng,Lanyang Wang,Yongqi Li
In the quest to enhance photocatalytic performance, structural regulation and interface engineering stand out as effective strategies for amplifying active sites and fostering the separation of photogenerated electron-hole pairs. In this study, a pioneering approach was employed, utilizing a straightforward two-step calcination method to prepare a novel photocatalyst : porous graphite carbon nitride (g-C 3 N 4 , CN) nanosheets (NS) loaded with mesoporous cerium oxide (CeO 2 /CNNS). The distinctive architecture of CeO 2 /CNNS not only elevates the specific surface area and catalytic active sites but also facilitates efficient mass transfer. Experimental results and density functional theory (DFT) calculations corroborate the existence of an internal electric field (IEF) within the Z-scheme heterojunction formed by uniformly dispersed CeO 2 and CNNS. This IEF proves pivotal in promoting charge separation and migration, thereby maintaining robust redox capabilities. Under visible light irradiation, CeO 2 /CNNS demonstrates an impressive 4.2-fold increase in the hydrogen production rate compared to bulk g-C 3 N 4 (BCN), coupled with a 48.5% boost in the photocatalytic degradation rate constant (k) of tetracycline (TC). This study not only unveils the innovative design of CeO 2 /CNNS but also paves the way for a fresh perspective on the design and preparation of multifunctional photocatalysts.
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