IONICS
·
2023
·Vol. 30
·期 3
·1677-1690
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IF 2.8
Ye Jiankang,Fu Qingshan,Ye Ang,Xu Changkangle,Zhou Feiyu,Chen Xuedan,Zhu Wenli,Chen Jian
Continuous efforts are always being made to reinforce biochars’ properties for large-scale utilization in new energy devices. Here, a pre-burning treatment is introduced for preparing the biochars from shaddock peel, longan pericarp, and loofah sponge. The results of XRD demonstrate more disordered structures are caused in the prepared biochars by the pre-burning, which also introduces more oxygen-containing functional groups on the biochars’ surface discovered by the FTIR and XPS tests. The N 2 adsorption–desorption results indicate that the pre-burning can facilitate pore formation in the biochars, boosting specific surface area and pore volume, significantly reducing the average pore size of biochars. Specifically, for the loofah sponge based biochar, the pre-burning treatment causes an increase in specific surface area (from 271 to 973 m 2 g −1 ), a rise in pore volume (from 0.22 to 0.53 cm 3 g −1 ), and a decrease in average pore diameter (from 3.21 to 2.3 nm). The specific capacitance of the supercapacitor with the loofah sponge based biochar electrodes is raised from 85.4 to 170 F g −1 at 0.5 A g −1 by the pre-burning treatment. Besides, the longan pericarp-derived biochar with the pre-burning treatment achieves a power density of 5000 W kg −1 under an energy density of 21.81 Wh kg −1 . We believe the pre-burning treatment is an effective and convenient route for the reinforcement of biochars.
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TOXICOLOGY AND APPLIED PHARMACOLOGY
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2025
·
IF 3.4
Hualu Wu,Haoxin Fu,Fangyuan Xia,Jun Wu,Peiqi Wang,Lu Cao,Ren-ai Xu,Lu Shi
Cariprazine is an antipsychotic medication that has been approved for the treatment of schizophrenia and manic or mixed episodes. Patients with tumors frequently develop psychiatric disorders, necessitating the combination of antitumor and antipsychotic drugs. The objective of the present study was to examine the inhibitory impacts of three antitumor drugs (olmutinib, napabucasin and ponatinib) on the metabolism of cariprazine, and the molecular docking of cariprazine and ponatinib in relation to CYP3A4 was also evaluated. As the results, the half-maximal inhibitory concentration (IC 50 ) values of ponatinib and olmutinib in vitro were < 10 μM, whereas napabucasin was >20 μM. Among these, ponatinib exhibited the smallest IC 50 value. The metabolic stability of cariprazine was observed in the presence or absence of ponatinib in rat liver microsomes (RLM). The IC 50 shift experiments demonstrated that the inhibition of cariprazine by ponatinib was non-time-dependent. In addition, ponatinib was shown to inhibit cariprazine in a mixed manner (RLM) and a competitive manner (HLM), respectively. In the in vivo study, the co-administration of ponatinib resulted in a significant 0.35-fold increase in both AUC (0-t) and AUC (0-∞) for cariprazine, accompanied by a significant 0.25-fold decrease in the CL z/F . Furthermore, the metabolites desmethyl-cariprazine (DCAR) and didesmethyl-cariprazine (DDCAR) exhibited disparate increases in both AUC (0-t) and AUC (0-∞) . Molecular docking studies had demonstrated that both cariprazine and ponatinib could engage in hydrophobic interactions with residue PHE-304 on CYP3A4. Consequently, when ponatinib is employed in conjunction with cariprazine in a clinical setting, it is imperative to assess the efficacy and adverse effects, and adjust the dosage to attain the optimal efficacy.
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Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Shuya Rui,Na Liu,Lingwei Ren,Yang Liu,Zian Liu,Binghui Wu,Hua Pan,Zhiping Ye
Traditional Mn-Ce catalysts for selective catalytic reduction (SCR) of NO with CO (CO-SCR) in iron and steel flue gases are often hindered by competitive adsorption of NO and CO, which compromises catalytic efficiency. In this study, we present a Ni-doped Mn-Ce catalyst that overcomes these challenges, achieving 96 % NO conversion, 98 % N 2 selectivity, and 44 % CO conversion at 175 °C. Comprehensive characterization of the catalyst reveals that Ni doping significantly enhances Mn-Ce interactions, boosting oxygen vacancy density and promoting efficient redox cycling between Mn 3 + /Mn 4+ and Ce 3+ /Ce 4+ . Diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) confirms preferential NO adsorption at oxygen vacancy sites, minimizing CO-NO competition and stabilizing the Langmuir-Hinshelwood mechanism, where both gases co-adsorb and react at oxygen-rich vacancies. Mechanistic insights suggest that Ni facilitates electron transfer, weakening the N–O bond in NO and accelerating its dissociation, resulting in faster NO reduction. This study establishes Ni doping as a promising strategy for improving the low-temperature performance of Mn-Ce-based CO-SCR catalysts, paving the way for more efficient catalytic systems.
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Food Bioscience
·
2025
·
IF 5.9
Qingqing Zhou,Yajun Yu,Zuorui Xie,Yingying Zhou,Yanan Li,Ping Li,Jiangning Hu,Qing Gu
Salmonella is a prevalent foodborne pathogen that poses a significant threat to public health. Our previous study identified Lactiplantibacillus plantarum ZJ316, a strain known for its production of indole-3-lactic acid (ILA), which exhibits a broad-spectrum bacteriostatic effect. This study aimed to explore the inhibitory mechanism of ILA against Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028 ( S . Typhimurium) both in vitro and in vivo , as well as to assess its potential to alleviate colitis induced by S . Typhimurium. In vitro experiments revealed that ILA exerts its ant- Salmonella effect by disrupting the transmembrane proton motive force (PMF) and influencing intracellular ATP synthesis. Subsequent experiments conducted in female C57BL/6 mice demonstrated that oral administration of 45.00 mg/L ILA for 21 d effectively reduced S. Typhimurium infection-induced upregulation of pro-inflammatory cytokines (IL-1β and CXCL15; P < 0.01 and P < 0.05, respectively), while promoting the production of anti-inflammatory cytokines (IL-10 and IL-22; P < 0.001). Compared to the control group, the ILA group had a 4.15% increase in the relative abundance of Firmicutes and a 3.95% decrease in Bacteroidota. Furthermore, ILA also effectively countered the reduction of short-chain fatty acids (SCFAs) such as acetic acid and propionic acid caused by S . Typhimurium. These findings indicate that ILA may possess the capability to suppress pathogens, enhance intestinal microecology, and facilitate the repair of intestinal tissue damage.
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CHEMICAL ENGINEERING SCIENCE
·
2025
·
IF 4.3
Xiaoting Feng,Chenlu Liu,Kunkun Ren,Xinglei Zhao,Longjie Liu,Keming Zhang,Xiaohe Tian,Qingnan Wang,Xiangyu Liu,Yueyangchao Yu,Tianhe Gu,Shaofei Wang
The urgent need for efficient CO 2 capture technologies to mitigate climate change has driven the advancement of membrane-based separation systems. We report a mixed matrix membrane design through the in-situ growth of a ZIF-8 gel network within a Pebax-1657 matrix. By tuning the solvent ratio and concentration of modulator, ZIF-8 gel rather than particles are formed within the polymer, establishing a three-dimensional interconnected MOF network that ensures homogeneous filler dispersion and creates continuous gas transport nanochannels. The optimized membrane achieves a remarkable CO 2 permeability of 183.9 Barrer and a CO 2 /N 2 selectivity of 73.3, surpassing the 2008 Robeson upper bound. These values represent 190.4 % and 34.9 % enhancements in permeability and selectivity, compared to the pristine polymer. The membrane also demonstrates robust operational stability and maintains > 95 % of its initial performance over 60 days. This work offers a promising solution to creating continuous channels in CO 2 separation membranes.
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Acta Geochimica
·
2025
·
IF 1.3
Wang Zhenglong,Shang Linbo,Chou I-Ming,Chen Chen,Zhou Yunhe,Li Jianguo,Jiang Ziqi,Gao Xinwei,Wan Ye
The formation of copper deposits is closely related to hydrothermal processes. Understanding the migration of copper in hydrothermal fluids aids in reconstructing mineralization processes and deciphering deposit genesis. Copper primarily exists as Cu + and Cu 2+ in hydrothermal solutions, with redox conditions governing their interconversion. In chloride-rich geological fluids, Cu–Cl complexes are considered critical for copper transport. However, the specific types and valence transitions of Cu–Cl complexes under varying hydrothermal conditions remain poorly understood. This study employed in situ Raman spectroscopy to systematically analyze Cu + HCl and CuCl 2 + K 2 S 2 O 3 /H 2 systems under saturated vapor pressure at 25–300 °C, elucidating the effects of temperature, Cl − concentration, and redox conditions on copper speciation. In the Cu + HCl system, copper dissolved as monovalent Cu–Cl complexes. At high temperatures (> 200 °C), [CuCl 2 ] − is the dominated species, whereas [CuCl 3 ] 2− becomes prevalent at lower temperatures and higher HCl concentrations. For the Cu 2+ –Cl system, the dominant species transitioned from [Cu(H 2 O) n ] 2+ (< 50 °C) to [CuCl 4 ] 2− (100 °C) and further to [CuCl] + and [CuCl 2 ] 0 at 300 °C. The introduction of reducing agents (K 2 S 2 O 3 /H 2 ) facilitated Cu 2+ → Cu + reduction, thereby stabilizing Cu + –Cl complexes and inducing partial copper precipitation. The behavior of copper in chloride-rich hydrothermal fluids observed in this study indicates that high-temperature oxidizing fluids facilitate Cu mobilization, while cooling and redox changes promote deposition and ore minerals formation.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·
IF 12.6
Zhe Zhang,Guangyu Jia,Qi Wang,Yamei Yu,Xiaolong Tang,Heqing Zheng,Xinyu Yang,Yao Xiao,Yangrui Ou,Jingjing Jiang,Hua Guo,Ye Wang,Shiyong Li
Intranasal drug delivery via nanocarriers has long been a research focus for enhancing drug concentration in the brain. However, the strategy of exploiting blood–brain barrier (BBB) alterations in atherosclerotic mouse models to enhance nanoparticle-mediated delivery of bromocriptine to the hypothalamus for the treatment of prolactinomas with hyperprolactinemia has not yet been reported. This study reveals that in patients with prolactinomas complicated by arteriosclerosis, bromocriptine therapy more effectively attenuates postoperative elevations in prolactin levels. In a mouse model, liposome-encapsulated bromocriptine efficiently traversed the nasal mucosa and entered the intracranial space. Compared with normal mice, bromocriptine-loaded liposomes delivered higher bromocriptine concentrations to the hypothalamus. Single-cell RNA sequencing revealed a significant upregulation of organic anion-transporting polypeptide 1a4 (Oatp1a4) expression in the brain endothelial cells of atherosclerotic mice. Importantly, bromocriptine-loaded liposomes more effectively reduced prolactin levels in a mouse model of prolactinoma with concurrent atherosclerosis. This study provides a theoretical foundation for the precision treatment of prolactinomas in arteriosclerosis.
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Plant Communications
·
2025
·
IF 11.6
Xin-He Yu,Xun Wen,Jiangqing Dong,Ya-Fang Hu,Xin-Long Wang,Dan-Yi Zhu,Qihua Ling,Hong-Yan Lin,Guang-Fu Yang
4-Hydroxyphenylpyruvate dioxygenase (HPPD) plays a critical role in plant photosynthesis, and is essential for enhancing tolerance to oxidative stress. However, the precise mechanisms through which plants regulate HPPD in response to oxidative stress remain largely unknown. Here, we report that the Arabidopsis thaliana HPPD ( At HPPD) undergoes an uncharacterized post-translational modification, namely phenylalanine hydroxylation, in response to excessive hydroxyl radicals (·OH), thereby mediating oxidative stress tolerance. Biochemical analyses reveal that this hydroxylation impairs the normal function of At HPPD, leading to its accelerated degradation. Furthermore, we identified PUB11 as a key interactor with At HPPD. In vitro and in vivo assays show that PUB11 enhances its interaction with At HPPD under oxidative stress, promoting ubiquitination and facilitating rapid degradation of At HPPD via the 26S proteasome pathway to balance the ROS levels. Overall, this work provides new insights into how plants balance photosynthetic efficiency and the repair of oxidative damage, while uncovering key processes in oxidative stress regulation, providing a foundation for crop breeding to meet abiotic challenges.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Buqing Wang,Yasen Wang,Xiaoman Zhou,Xiao-Dong Gao,Morihisa Fujita,Zijie Li
BACKGROUND Acidic lipases with high catalytic activities under acidic conditions have important application values in the food, feed and pharmaceutical industries. However, the availability of acidic lipases is still the main obstacle to their industrial applications. Although a novel acidic lipase Rasamsonia emersonii (LIPR) was heterologously expressed in Escherichia coli , the expression level was unsatisfactory. RESULTS To achieve the high-efficiency expression and secretion of LIPR in Pichia pastoris GS115, the combinatorial optimization strategy was adopted including gene codon preference, signal peptide, molecular chaperone co-expression and disruption of vacuolar sorting receptor VPS10 . The activity of the combinatorial optimization engineered strain in a shake flask reached 1480 U mL −1 , which was 8.13 times greater than the P. pastoris GS115 parental strain. After high-density fermentation in a 5-L bioreactor, the highest enzyme activity reached as high as 11 820 U mL −1 . LIPR showed the highest activity at 40 °C and pH 4.0 in the presence of Ca 2+ ion. LIPR exhibited strong tolerance to methanol, indicating its potential application in biodiesel biosynthesis. Moreover, the gastrointestinal digestion simulation results demonstrated that LIPR was tolerant to pepsin and trypsin, but its activity was inhibited by sodium taurodeoxycholate. CONCLUSION This study provided an effective approach for the high expression of acidic lipase LIPR. LIPR was more appropriate for lipid digestion in the stomach than in intestine according to the gastrointestinal digestion simulation results. © 2024 Society of Chemical Industry.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 4
·1-12
·
IF 5.3
Liu Mengyue,Xu Shicai,Marrazza Giovanna
Multivalent engineering aptamers (multi-Apts) adaptive and specific to whole Escherichia coli ( E. coli : CMCC 44102) cells based on hybridization chain reaction (HCR) were constructed for the first time. The dissociation constant ( K d ) value for these multi-Apts was 48 nM, demonstrating a higher affinity than that of monovalent aptamers (mono-Apts) ( K d = 102 nM). Furthermore, the reaction equilibrium of multi-Apts was achieved within 20 min, with a reaction rate twice that of mono-Apts. To validate the exceptional performance of these multi-Apts, they were employed as recognition elements in conjunction with gold nanoparticles (AuNPs) colorimetric assays for the all-in-one rapid detection of E. coli . This method exhibited a linear detection range from 1 × 10 2 to 1 × 10⁷ CFU mL⁻ 1 , achieving a limit of detection (LOD) as low as 19 CFU mL⁻ 1 . The recovery of this method in tap water and milk were 85.7% to 101% and 81.8% to 98.2%, respectively. The results indicated that this method not only provided a wide detection range but also exhibited high sensitivity and accuracy. Additionally, this study demonstrated that multi-Apts possessed greater application potential in the detection of macromolecular substances such as bacteria. In short, this work provided a novel approach for rapid and all-in-one detection of E. coli in food. Graphical
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JOURNAL OF LIPOSOME RESEARCH
·
2021
·
IF 3.648
Hua Nie,Bo Qiu,Qi-Xuan Yang,Ying Zhao,Xiao-Min Liu,Ying-Ting Zhang,Fu-Lin Liao,Sheng-Yuan Zhang
In this study, we describe a novel synthesis of galactosylated lipids by lipase catalysis. Lactitol (Lac), galactose (Gal), or N-acetyl galactosamine (GalNAc) was coupled with cholesterol (CHS) as target head groups by enzyme-catalyzed regioselective esterification to produce three kinds of lipids: CHS-1-Gal, CHS-6-Gal, or CHS-6-GalNAcFootnote1. The biological effects of galactosylated lipids carrying different constitutional isomers of the pendent sugar species were investigated. LP-1-Gal (liposomes containing 5.0 molar% of CHS-1-Gal) showed strong binding to tetrameric lectins of Ricinus communis agglutinin (RCA120) in vitro, while LP-6-Gal (liposomes containing 5.0 molar% of CHS-6-Gal) and LP-6-GalNAc (liposomes containing 5.0 molar% of CHS-6-GalNAc) did not. After intravenous injection, LP-6-GalNAc, LP-1-Gal and LP-6-Gal rapidly disappeared from the blood and accumulated rapidly in liver (up to 74.88 ± 4.11%, 58.67 ± 5.75%, and 47.66 ± 4.56% of injected dose/g organ within 4 h, respectively). This is significantly higher than the uptake of unmodified liposomes (Unmod-LP) (18.67 ± 6.07%). Pre-injection of asialofetuin significantly inhibits liver uptake of Gal-liposomes (P < 0.01), with the degree of inhibition appearing in the following order: LP-6-GalNAc (73.29%) > LP-1-Gal (67.06%) > LP-6-Gal (53.61%). More importantly, LP-6-GalNAc was preferentially taken up by hepatocytes and the uptake ratio by parenchymal cells (PC) and nonparenchymal cells (NPC) (PC/NPC ratio) was 11.03 higher than LP-1-Gal (7.32), LP-6-Gal (5.83) and Unmod-LP (2.39). We suggest that liposomes containing the novel galactosylated lipid CHS-6-GalNAc have potential as drug delivery carriers for hepatocyte-selective targeting.
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International Journal of Nanomedicine
·
2024
·
IF 6.6
Lingling Gong,Tao Jiang,Ting Xiao,Bo Feng,Mouda Wei,Chuanzi Liu,Weiwei Xiao,Pin Huang,Dan Huang
Introduction Biomineralization has been increasingly adopted for the synthesis of advanced materials with superior properties. Hierarchical architecture growth mimicking biomineralization has been studied using various organic molecules to template inorganic materials with controlled morphology. In our previous study, self-assembled Sr/CS/G(SrCO3-chitosan-gelatin) aggregates were fabricated using electrophoretic deposition (EPD). This study is a further step toward understanding the morphogenesis of Sr/CS/G aggregates and its biomineralization.Methods Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) were used to investigate the biomimetic morphogenesis of Sr/CS/G composite under various EPD parameters, such as polymer concentration, time, and voltage. The Sr/CS/G aggregates were immersed in H2O, phosphate-buffered saline (PBS), and simulated body fluid (SBF) to study the bioactive apatite formation ability. In addition, biocompatibility of the composites were evaluated by Fluorescence staining, SEM in vitro. The osteogenic ability of the coatings induced by PBS were tested in vivo.Results The CS/G weight ratio, EPD time, and voltage were found to influence the morphogenesis of Sr/CS/G aggregates. SEM and TEM results showed that the Sr/CS/G aggregates exhibited fractal growth characteristics and morphological self-similarity. XRD results confirmed the formation of SrCO3 crystals within the framework of chitosan and gelatin organic templates. Chitosan played a vital role in branching growth of the crystals, whereas gelatin guided the formation of composite spheres. The microstructural and compositional results reveal that the Sr/CS/G-induced apatite coating yielded a large quantity of apatite. These apatite coatings promote the cytocompatibility and osteogenesis of rat bone marrow mesenchymal stem cells (rBMSCs) in vitro. The coatings induced by PBS enhanced proliferation and mineralization in vitro, and enhanced angiogenesis and osteogenesis in vivo.Conclusion Sr/CS/G composites prepared via EPD are promising organic-inorganic templates for biomineralization. These findings provide important insights into understanding the mineralization process and optimizing the design of advanced biological materials.
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