Foods
·
2024
·Vol. 13
·期 11
·1705
·
IF 4.7
Jin Zhang,Jinling Lv,Guodong Zhuang,Junjia Zhang,Feng Hu,Yongsheng Chen
Recent research has demonstrated the positive impact of herbal tea consumption on postprandial blood glucose regulation. This study conducts a comparative analysis of aqueous and ethanol extractions on four herbal teas (Mallotus, Cyclocarya, Rubus, and Vine) to assess their phytochemical profiles and functional attributes. Phytochemical contents, antioxidant activities, α-glucosidase inhibitory activities, and chemical compositions are investigated via colorimetric analyses and UPLC-Q-Orbitrap HRMS/MS, respectively. Results indicate that Vine, among the teas studied, exhibits the most pronounced glucose-regulating effects under both extraction methods. While ethanol extractions yield higher phytochemical content overall, the compositions vary. Conversely, aqueous extracts demonstrate unexpectedly potent antioxidant activities and comparable α-glucosidase inhibitory activities to ethanol extracts. Phytochemical contents correlate positively with antioxidant activities and α-glucosidase inhibitory activities. However, antioxidant activities exhibit a weak positive correlation with α-glucosidase inhibitory activities. These findings provide evidence that aqueous extracts from herbal teas contain valuable phytochemical compositions beneficial for antioxidants and individuals with hyperglycemia, suggesting their potential as functional ingredients to enhance the nutritional value of herbal food products.
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PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Songjie Li,Ruimin Wang,Xiaoping Hu,Congfa Li,Lu Wang
Cerasus humilis (Bge.) Sok. leaf-tea (CLT) has a potential anti- α -glucosidase effect. However, its anti- α -glucosidase functional compositions remain unclear. Results showed that 70% methanol extract of CLT (IC 50 = 36.57 μg/mL) with the highest total phenolic/flavonoid contents exhibited significantly higher α -glucosidase inhibitory activity ( α -GIA) than acarbose (IC 50 = 189.57 μg/mL). Additionally, phenolic constituents of the CLT extract were analyzed for the first time in this work. Ten major potential α -glucosidase inhibitors ( α -GIs) with high bio-affinity degree in the CLT extract were recognized using a bio-affinity ultra-filtration and HPLC-ESI-qTOF-MS/MS method. In vitro α -GIA assay confirmed that myricetin (IC 50 = 36.17 μg/mL), avicularin (IC 50 = 69.84 μg/mL), quercitrin , isoquercitrin, prunin and guajavarin were responsible for the α -GIA of the CLT extract. More importantly, the interaction mechanism between α -GIs and α -glucosidase was investigated via in silico analysis. This study provides a high-throughput screening platform for identification of the potential α -GIs from natural products.
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高影响因子
JOURNAL OF CEREAL SCIENCE
·
2022
·
IF 4.075
Xiangqing Ji,Cheng Zeng,Dingkuan Yang,Siyu Mu,Yu Shi,Yicheng Huang,Byung-Hoo Lee,Dan Li,Xiaolei Li
Consumers significantly prefer wheat noodles that offer both an acceptable texture and health benefits. To this end, a cold-active 1,4-α-glucan branching enzyme from Bifidobacterium longum (BlBE) was expressed in the generally recognized as safe (GRAS) host cell Bacillus subtilis and then used at 120, 240 or 480 U/g flour in the preparation of raw dough. The storage modulus and loss modulus of the BlBE-modified dough decreased, but the pseudo-plasticity increased. The cooked noodles' hardness, gumminess and chewiness decreased by 12.1%–45.8%, 9.7%–40.4% and 16.0%–42.4%, respectively, but both resilience and cohesiveness increased by 4.0%–14.8% and 1.8%–9.0%, respectively. Pearson analysis revealed a correlation between the noodles' textural parameters and the doughs' power law equation parameters. BlBE-modified noodles exhibited significantly (P < 0.05) lower differential scanning calorimetry retrogradation enthalpies during storage at 4 °C for 7 days and released significantly lower levels of glucose during incubation with mammalian α-glucosidase at 37 °C for 10 min–360 min. BlBE at 480 U/g flour increased the slowly digestible portion by 12.6%. BlBE decreased the molecular weight, x-ray diffraction crystallinity, and fourier transform infrared spectroscopy ratio of band's heights at 1045 cm −1 and 1022 cm −1 of starch but increased the amylopectin content and ratio of short- to long-branch chains. These structural changes in starch partially explained the reduced retrogradation and enhanced slowly digestible starch fraction in the noodles.
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高影响因子
JOURNAL OF CHEMICAL RESEARCH
·
2023
·
IF 1.4
Mingguang Zhang,Wenlong Wang,Cheng Guo,Chunhuan Jiang
Alkyl-substituted testosterone derivatives are promising platforms for new drug discovery in medicinal chemistry. This approach provides a simple and efficient method for introducing an alkyl substituent to steroids at the C-4 position. In this study, the novel compound 3α-hydroxy-3β-methyl-4,4-dimethyl-5α-21-bromo-19-nor-pregnan-20-one is synthesized from 19-nor-testosterone. The protocol involves methylation of the dienolate, reduction of the alkene, the Grignard reaction of the carbonyl group, a Wittig reaction, hydroboration with BH3, the oxidation and bromination with a 49% overall yield. For the methylation and reduction steps, the effects of the base, solvent, and reactant ratio on the conversion and yield are investigated. The structures of the synthesized compounds are determined by nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS) (electrospray ionization (ESI)).
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高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2015
·Vol. 38
·期 9
·1499-1506
·
IF 2.737
Zaifa Pan,Xiaoya Huang,Yuan Zhong,Lili Wang,Danhua Zhu,Lanjuan Li
The determination of α-ketoacid concentration is demanded to evaluate the absorption and metabolic behavior of compound α-ketoacid tablets taken by chronic kidney disease patients. To eliminate the interference of endogenous substance of urine and enrich the analytes, a three-phase hollow-fiber liquid-phase microextraction combined with ion-pair high-performance liquid chromatography method was established for the determination of d , l -α-hydroxymethionine calcium, d , l -α-ketoisoleucine calcium, α-ketovaline calcium, α-ketoleucine calcium, and α-ketophenylalanine calcium of compound α-ketoacid tablets in human urine samples. The extraction parameters, such as organic solvent, pH of donor phase and acceptor phase, stirring rate, and extraction time were optimized. Under the optimal conditions, the obtained enrichment factors were up to 11-, 110-, 198-, 202-, and 50-fold, respectively. The calibration curves for these analytes were linear over the range of 0.1–10 mg/L for α-ketovaline calcium, d , l -α-ketoisoleucine calcium, and α-ketoleucine calcium, 0.5–10 mg/L for d , l -α-hydroxymethionine calcium, and α-ketophenylalanine calcium with r > 0.99. The relative standard deviations ( n = 5) were less than 6.27% and the LODs were 100.7, 10.0, 5.8, 7.8, and 8.6 μg/L (based on S/N = 3), respectively. Good recoveries from spiked urine samples (92–118%) were obtained. The proposed method demonstrated excellent sample clean-up and analytes enrichment to determine the five components in human urine.
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高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2024
·
IF 20.2
Zhenyu Yan,Wenyu Gao,Chenkai Zhong,Qiangqiang Jiao,Shujie Tian,Jia Liu
Ammonia (NH 3 ) plays an important role in agricultural production and is also an ideal zero-carbon energy source in sustainable hydrogen economy. Electrocatalytic nitrate (NO 3 - ) reduction reaction can simultaneously consume nitrogen pollutants in water during the synthesis of NH 3 , which is the most promising and gentle alternative process to the Haber-Bosch. However, due to the interference of hydrogen evolution reaction and high overpotential, the development of efficient directional ammonia conversion catalysts has become the key. In this study, Mo-Fe 2 O 3 material were fabricated by crystal face engineering and orbital manipulation strategies. The (001) face of α-Fe 2 O 3 with the best electrochemical performance was exposed by thermodynamic control, and the in-situ substitution of Mo single atoms for near-surface Fe atoms was further used to achieve excellent ammonia synthesis activity. NH 3 yield of 493.67 μg/h/mg cat and Faraday efficiency of 95.0% were achieved at -0.5 V vs. RHE (pH=7, 0.1 M PBS+500 ppm NO 3 - ). The mechanistic analysis showed that the highly electronegative Mo atoms induced strong interaction by Fe-O-Mo bond, which prompted Fe(III) to preferentially fill the lower t2g orbital and form a low-spin state. The weaker anti-bonding state enhances the interaction between Fe(III) and the coordinating O atoms in NO 3 - , thereby strengthening electron transfer. In summary, the targeted regulation of the crystal facet and electronic orbitals of catalysts presents a promising strategy for enhancing the conversion of NO 3 - to NH 3 .
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高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
·
IF 5.4
Xiangta Xie,Yuyan Feng,Dongbo Wang
A novel in-situ grown α-Bi 2 O 3 /Bi 2 O 2 CO 3 /g-C 3 N 4 heterojunction (BBCx) was successfully fabricated via a two-step in-situ growth by a simple solvothermal calcination method. In this process, g-C 3 N 4 served as the substrate for the growth of Bi 2 O 2 CO 3 nanoplates, while Bi 2 O 2 CO 3 acted as the substrate for the subsequent growth of α-Bi 2 O 3 nanoplates. By characterizing the nanocomposite using different techniques, it was confirmed that the components of this ternary material are tightly bonded through heterojunction formation. Photocatalytic tests demonstrated that the composite exhibited higher activity than pure α-Bi 2 O 3 , Bi 2 O 2 CO 3 and g-C 3 N 4 . Among the composite materials, BBC400—obtained by solvothermal treatment followed by calcination at 400 °C for 2 h—exhibited the highest degradation efficiency toward tetracycline hydrochloride (TC-HCl), with a removal rate of 70% of TC-HCl within 120 min and a rate constant of 0.0081 min −1 . This rate constant was approximately 16.2 times (0.0005 min −1 ), 1.92 times (0.0042 min −1 ) and 1.35 times (0.006 min −1 ) higher than those of pure Bi 2 O 2 CO 3 , g-C 3 N 4 and α-Bi 2 O 3 , respectively. This synthesis approach offers a new strategy for the in-situ construction of heterojunction nanocomposites.
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高影响因子
Frontiers in Pharmacology
·
2025
·
IF 4.8
Yeshi Yin,Changhui Zhao,Qin Xiang,Zongyan Li,Xiu Liu,Changhui Hu,Rong Yu
Background: Animal and cell studies have demonstrated that Zuogui-Jiangtang-Yishen decoction (ZGJTYS) has a favorable effect on the treatment of diabetic kidney disease (DKD). Our previous clinical research also showed that ZGJTYS prevents DKD in a manner similar to that of benazepril. Nevertheless, the interactions between ZGJTYS and the human gut microbiota require further investigation, particularly its interference in the intestinal flora response to food ingredients that may increase DKD risk, such as L-α-phosphatidylcholine and L-tyrosine.Objective: The aim of this study was to evaluate the regulatory function of ZGJTYS on human gut microbiota and explore the effect of ZGJTYS on the intestinal flora response to L-α-phosphatidylcholine and L-tyrosine.Methods: ZGJTYS was prescribed from the First Affiliated Hospital of Hunan University of Chinese Medicine. High-throughput sequencing of bacterial 16S RNA genes and fungal internal transcribed spacer (ITS) sequences was used for intestinal flora analysis. An in vitro gut microbiota simulation model was used to investigate the effect of ZGJTYS on the intestinal flora’s response to L-α-phosphatidylcholine and L-tyrosine. Ultra-high-performance liquid chromatography and mass spectrometry were used for non-targeted metabolomics analysis.Results: Compared to the control group, the microbial diversity of DKD was significantly reduced by ZGJTYS treatment; three bacterial genera, including Parabacterioids, were significantly higher; eight bacterial genera, including Prevotella_9, and the linoleic acid content were significantly lower. A receiver operating characteristic curve analysis using Parabacterioids and Prevotella_9 showed an area under the curve greater than 0.75, indicating good predictive performance. ZGJTYS intervention restored some of the normal bacterial genera, such as Rickettsia and Metarhizium, which were regulated by L-α-phosphatidylcholine and L-tyrosine. Furthermore, ZGJTYS effectively restored several significantly different Kyoto Encyclopedia of Genes and Genomes metabolic pathways related to immunity and disease to normal, such as efferocytosis and tryptophan metabolism.Conclusion: ZGJTYS was found to effectively restore the microbiota that were altered by L-α-phosphatidylcholine and L-tyrosine to normal, along with their metabolites. However, the mechanism by which ZGJTYS exerts its preventive and therapeutic effects on DKD through the gut microbiota still requires further study.
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PubMed
高影响因子
Bioengineered
·
2021
·
IF 3.269
Zhenqiang Li,Chengfeng Sun,Maosong Chen,Boding Wang
A recent study has reported that lumican (LUM) is expressed at a high level in the nucleus pulposus specimens from herniated lumbar disc, without description of the specific mechanism. This study was designed to investigate the function and mechanism of LUM in intervertebral disc degeneration (IDD). In this study, human nucleus pulposus cells (hNPCs) cells were challenged with tumor necrosis factor (TNF)-α to establish the IDD in vitro model. After LUM silencing, cell viability was detected using CCK-8 kit, and the expression of inflammatory factors was evaluated using RT-qPCR and ELISA. Flow cytometry and β-galactosidase staining were used to determine cell cycle and cell senescence. The expression of cycle and senescence-related proteins was evaluated with western blotting. Then, Fas ligand (FasL) was overexpressed and proteins in apoptosis signal regulating kinase 1 (ASK1)/p38 signaling were tested. Finally, GS-4997, an inhibitor of ASK1, was used to explore the regulatory effects of LUM on ASK1/p38 signaling in TNF-α-induced hNPCs. Results indicated that LUM expression was upregulated in TNF-α-challenged hNPCs. LUM gene interference mitigated TNF-α-induced inflammatory response, cell cycle arrest, and senescence of hNPCs. It was then found that LUM silencing could inhibit ASK1/p38 signaling in TNF-α-treated hNPCs, which was reversed by FasL overexpression. Additionally, ASK1/p38 participated in the mediation by LUM of TNF-α-induced inflammation, cell cycle arrest, and senescence of hNPCs. To conclude, interference with LUM effectively mitigated TNF-α-induced inflammatory response, cell cycle arrest, and cell senescence. Further experiments showed the involvement of ASK1/p38 pathway in LUM-mediated NP cell phenotypes through FasL.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Jixu Wu,Huarong Shao,Jinhua Zhang,Yong Ying,Yanling Cheng,Dan Zhao,Xixi Dou,Huimin Lv,Shuaiguang Li,Fei Liu,Peixue Ling
We isolated and characterized a Mussel polysaccharide, α -D-glucan (MP-A), from Mytilus coruscus earlier. In this work, the pharmacological activity and mechanisms of MP-A as an oral supplement for non-alcoholic fatty liver disease (NAFLD) were explored. High fat diet (HFD) was utilized to induce NAFLD in Sprague Dawley male rats and MP-A (0.6 g/kg) was supplemented for 4 weeks. The results showed that MP-A supplementation reduced blood lipid levels, intrahepatic lipid accumulation and NAFLD activity score in HFD-fed rats. Additionally, the analysis of 16S rDNA sequencing on gut microbiota samples revealed that HFD could induce microbial dysbiosis. However, MP-A supplementation could remodel gut microbiota structure, inhibit LPS-TLR4-NF- κ B pathway activation, and restrain subsequent inflammation factors secretion. Furthermore, MP-A regulated the lipid metabolism by promoting the production of short chain fatty acids and suppressing PPAR γ and SREBP-1c expression. Our results support that MP-A can prevent against NAFLD and act as an oral supplementation for hepatoprotection via modulating gut microbiota and related gut-liver axis signaling pathways.
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PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 12
·2565-2574
·
IF 2.557
Senlin Li,Sainan Li,Yu Huang,Chunming Liu,Lina Chen,Yuchi Zhang
The separation of a compound of interest from its structurally similar homologues to produce high-purity natural products is a challenging problem. This work proposes a novel method for the separation of iristectorigenin A from its structurally similar homologues by ionic-liquid-based ultrasound-assisted extraction and the subsequent screening and isolation of potential α-glucosidase inhibitors via ultrafiltration and semipreparative high-performance liquid chromatography. Ionic-liquid-based ultrasound-assisted extraction was successfully applied to the extraction of tectorigenin, iristectorigenin A, irigenin, and irisflorentin from Belamcanda chinensis . The optimum conditions for the efficient extraction of isoflavones were determined as 1.0 M 1-ethyl-3-methylimidazolium tetrafluoroborate with extraction time of 30 min and a solvent to solid ratio of 30 mL/g. Ultrafiltration with liquid chromatography and mass spectrometry was applied to screen and identify α-glucosidase inhibitors from B. chinensis , followed by the application of semipreparative high-performance liquid chromatography to separate and isolate the active constituents. Four major compounds including tectorigenin, iristectorigenin A, irigenin, and irisflorentin were screened and identified as α-glucosidase inhibitors, and then the four active compounds abovementioned were subsequently isolated by semipreparative high-performance liquid chromatography (99.89, 88.97, 99.79, and 99.97% purity, respectively). The results demonstrate that ionic liquid extraction can be successfully applied to the extraction of isoflavones from B. chinensis .
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PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 78
·74903-74912
·
IF 3.289
Xiaoyan Li,Zhengzhou Wang,Lixin Wu,Tsungyen Tsai
A novel α-zirconium phosphate/graphene oxide hybrid (ZGO) with α-zirconium phosphate (α-ZrP) nanoparticles immobilized on the surface of graphene oxide (GO) sheets was successfully synthesized by a one-step in situ method, and characterized by X-ray diffraction (XRD), thermogravimetric analysis (TGA), FTIR spectroscopy and transmission electronic microscopy (TEM). TGA results showed that the modification of α-ZrP nanoparticles on the GO surface significantly improved the thermal stability of the GO. Subsequently, the ZGO was incorporated into phenolic (PF) foams for reducing their high brittleness and friability. At a ZGO dosage of 4 phr (parts per hundreds of resin), the flexural and compressive strengths of the ZGO-toughened PF foams increased by 33.8% and by 39.6%, respectively, and the pulverization ratio decreased by 57.5%, compared with the pure PF foam. Besides, the results of the TGA test, limiting oxygen index (LOI) and cone calorimetric test demonstrated that the addition of ZGO imparted excellent thermal stability and flame retardancy to the PF foam, which was mainly attributed to the synergistic effects between α-ZrP nanoparticles and GO.
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高影响因子
危险品化学品经营许可证（带存储）