ENZYME AND MICROBIAL TECHNOLOGY
·
2024
·
IF 3.4
Dingyu Guo,Zhengchao Wang,Wanqing Wei,Wei Song,Jing Wu,Jian Wen,Guipeng Hu,Xiaomin Li,Cong Gao,Xiulai Chen,Liming Liu
D-allulose is a naturally occurring rare sugar and beneficial to human health. However, the efficient biosynthesis of D-allulose remains a challenge. Here, we mined a new D-tagatose 3-epimerase from Kroppenstedtia eburnean (KeDt3e) with high catalytic efficiency . Initially, crucial factors contributing to the low conversion of KeDt3e were identified through crystal structure analysis, density functional theory calculations (DFT), and molecular dynamics (MD) simulations. Subsequently, based on the mechanism, combining restructuring the flexible region, proline substitution based onconsensus sequence analysis, introducing disulfide bonds , and grafting properties, and reshaping the active center, the optimal mutant M5 of KeDt3e was obtained with enhanced thermostability and activity. The optimal mutant M5 exhibited an enzyme activity of 130.8 U/mg, representing a 1.2-fold increase; T m value increased from 52.7 °C to 71.2 °C; and half-life at 55 °C extended to 273.7 min, representing a 58.2-fold improvement, and the detailed mechanism of performance improvement was analyzed. Finally, by screening the ribosome-binding site (RBS) of the optimal mutant M5 recombinant bacterium (G01), the engineered strain G08 with higher expression levels was obtained. The engineered strain G08 catalyzed 500 g/L D-fructose to produce 172.4 g/L D-allulose, with a conversion of 34.4% in 0.5 h and productivity of 344.8 g/L/h on a 1 L scale. This study presents a promising approach for industrial-scale production of D-allulose.
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高影响因子
MATERIALS RESEARCH BULLETIN
·
2023
·
IF 5.4
Xuewen Peng,Bin Yang,Danjiang Deng,Zhiwei Cai,Xi Kong,Letao Yang,Jinming Guo
The application of lead-free dielectric ceramics for energy storage has received extensive attention because of their remarkable potential as pulse capacitors in power electronic devices. The current study developed a strategy for improving the energy storage performance of (K 0.5 Na 0.5 )NbO 3 (KNN)-based ceramics by incorporating Bi(Zn 2/3 Nb 1/3 )O 3 to reduce grain size, increase dielectric breakdown strength, and enhance ferroelectric relaxation degree. The resulting 0.87KNN–0.13BZN ceramics exhibited a high energy density (7.4 J/cm 3 ) and moderate energy efficiency (74%) at an ultrahigh electric field (750 kV/cm) as well as excellent optical properties. Therefore, the 0.87KNN–0.13BZN ceramic is a promising candidate for pulse capacitors, providing a feasible approach for the development of new lead-free energy storage materials.
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INORGANICA CHIMICA ACTA
·
2021
·
IF 2.545
Xingliang Li,Juncai Lu,Wanjun Mu,Baihua Chen,Daibing Luo,Bijun Liu,Yanqiu Yang,Hongyuan Wei,Shuming Peng
Nitrilotri–3–propanoic Acid (NTPA) and Tris(2–carboxyethyl) phosphine (TCEP) possess similar skeleton. This pair of isostructural ligands is a perfect set to assess the differences of uranyl (UO 2 2+ ) complexes with N–tricarboxylate versus P–tricarboxylate. The protonation reactions of NTPA and TCEP were investigated by potentiometry and calorimetry. Complexation of uranyl with the both ligands was studied by potentiometry and structural analysis. The results indicate that central trialkylamine in NTPA has a higher affinity for the proton than trialkylphosphine in TCEP. Crystal structures and NMR analyses indicate that central amine of NTPA and central phosphine of TCEP are not involved in the bonding. NTPA/UO 2 2+ complex is more stable than TCEP/UO 2 2+ complex. This difference cannot be explained by electronic effect of central N and P atoms because of nonbonding interactions. The higher stability of UO 2 2+ /NTPA versus UO 2 2+ /TCEP may be due to the fact that the central N atom of NTPA has a lower steric hindrance than the central P atom of TCEP. Another possible reason is that UO 2 2+ /TCEP complexes have different coordination modes from UO 2 2+ /NTPA complexes.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Jinhong Song,Chen Zhang,Zejian Zheng,Shizhe Huo,Yihan Lin,Fei Yang,Lei Liu
SnO 2 layer between Li 1.5 Al 0.5 Ge 1.5 (PO 4 ) 3 (LAGP) and lithium anode was prepared through simple scratch-coating process to improve interface properties. The physical phase, morphology, and electrochemical properties of Li/SnO 2 /LAGP structure were characterized by X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, and electrochemical analytical methods. It was found that SnO 2 layer effectively improved the interface stability of LAGP and lithium anode. The prepared Li/SnO 2 /LAGP/SnO 2 /Li symmetric cell exhibited a large critical current density of 1.8 mA cm −2 and demonstrated excellent cycling characteristics. The polarization voltages of symmetric cell were 0.1 V and 0.8 V after 1000 h of cycling at current densities of 0.04 mA cm −2 and 0.5 mA cm −2 , respectively. Li/SnO 2 @LAGP/LiFePO 4 solid-state full cells were also assembled, exhibiting a discharge specific capacity of 150 mAh g −1 after 200 cycles at 0.1C with capacity retention rate of 96 %. The good interface properties of Li/SnO 2 /LAGP structure are attributed to the transformation of SnO 2 layer into a buffer layer containing Li 2 O, Sn 0 , and Li x Sn y alloy during cycling process, which effectively inhibits the reduction reaction between LAGP and lithium anode.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Mingwei Zhao,Mengjiao Cao,Yong Hu,Qianru Yang,Yining Wu,Zhongzhong Chu,Xuewen Hu,Caili Dai
Polymer as non-Newtonian fluids is widely used in oilfield flooding for enhanced oil recovery (EOR). The study on the oil droplet behaviors in polymer solution has a remarkable influence for clarifying the EOR mechanism of polymer flooding. In this paper, factors influencing the oil droplet breakup process, including polymer concentration, pore throat structure, elasticity as well as flow rate, were investigated. It was found that the oil droplets were liable to breakup and the average size of daughter droplet decreased by about a quarter as the polymer concentration increased (from 0.01% to 0.5%). A prediction model for the effect of polymer concentration on the length of the first daughter droplet was established. Furthermore, the relationship between polymer concentration and the length of the first daughter droplet was fitted to an exponential function. After excluding the influence of viscosity, it was found that the elastic property of polymer fluid made the oil droplets more likely to breakup when passing through pore throat.
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高影响因子
INORGANIC CHEMISTRY
·
2020
·
IF 4.825
Siru Guo,Wenbin Zhang,Min Zhang,Zhihua Yang,Shilie Pan
Owing to the tendency to form the glass state in growing crystals in the ternary tin(II) borate system, hitherto, very few single crystals in ternary tin(II) borate were reported. In this paper, a single crystal of Sn3B8O15 was obtained via a high temperature solution method in a closed system. The structure features a flexible fundamental building block [B8O18]12– constructed by one [BO3]3– unit and one [B7O16]11– group that contains three hexatomic rings, which further connect to compose a two-dimensional 2∞[B8O15] layer structure. The connection mode and flexibility of the three hexatomic B-O rings were analyzed, verifying the structural diversity of the B-O configuration. Moreover, the synthesis, crystal structure, and various characterizations were comprehensively presented. Also, theoretical calculations were employed to discuss structure-property relationships, and we use the real-space atom-cutting techniques to explain the origin of the birefringence of Sn3B8O15.
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高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2024
·
IF 4.3
Mingxuan Zong,Yanjiang Zhang,Jianquan Liang,Xinghai Li,Qi Qi,Kangyi Chen,Li Zhao,Changsong Dai
The recently discovered Na 3 MnTi(PO 4 ) 3 cathode material has an extremely high theoretical capacity, but its poor cycling performance makes it challenging to apply. In this work, a series of NASICON-type Na 3+x MnV x Ti 1-x (PO 4 ) 3 /C (x = 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.8, 1) cathode materials were prepared by sol-gel method for sodium-ion batteries. X-ray diffraction (XRD) indicates that vanadium doping has increased the cell spacing within the material and the cell volume. From the results of X-ray photoelectron spectroscopy (XPS) and Energy dispersive X-ray spectroscopy (EDS) mapping images, it is shown that vanadium has been successfully doped into the material. Among them, the Na 3·3 MnV 0·3 Ti 0·7 (PO 4 ) 3 /C delivers an outstanding specific capacity (121.7 mAh g −1 at 0.1C, C = 135 mA g −1 ) and Na 3·4 MnV 0·4 Ti 0·6 (PO 4 ) 3 /C shows an excellent capacity retention (80.88 % after 1500 cycles at 1C). Notably, the coulombic efficiency of both materials during cycling is close to 100 %, indicating that these materials have great practical application value.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Yuanzhi Yue,Guozhe Sui,Yan Zhuang,Dongxuan Guo,Shuang Meng,Dantong Zhang,Xue Yang,Ning Liu,Yue Li,Jinlong Li
In this study, we developed a synergistic modulation strategy through in situ dual-anion/cation doping coupled with vacancy engineering, demonstrating a direct ammonia etching approach to synthesize Cu/S-In(OH) 3 with controlled indium vacancies (V In ) from CuInS 2 precursors. This in situ doping approach provides a uniform distribution of Cu cations and S anions throughout the material, overcoming conventional doping heterogeneity. Simultaneously, an indium vacancy (V In ) structure was precisely engineered and regulated through a growth limitation strategy. The synergistic interplay between the uniformly dispersed anions, cations and V In significantly suppresses the recombination of photogenerated carrier within In(OH) 3 , thereby allowing more carriers to participate in the surface catalytic reaction. Both experimental and theoretical analyses demonstrate that the introduction of V In into the defective states leads to a direct reduction in the band gap, significantly improving photocatalytic hydrogen evolution performance. The optimized Cu/S-In(OH) 3 with V In achieves a photocatalytic hydrogen production rate of 1.22 mmol g −1 h −1 , approximately 122 times higher than that of pure In(OH) 3 (0.01 mmol g −1 h −1 ). This work establishes a paradigm of integrating in situ doping and vacancy engineering strategies to rationally design In(OH) 3 -based materials, offering generalizable principles for advanced catalytic applications.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Dongxing Dong,Zhiqiang Cheng,Jingjing Zhang,Zhengyi Gu,Zhaolian Han,Wenli Hou,Xiaoli Lv
Pressure ulcer dressings with different functions can enhance wound healing ability to varying degrees; however, pressure ulcer dressings that integrate various functions and break the resistance of bacteria to traditional antibiotics have not been widely studied. We proposed a self-pumping/super-absorbent/transportable drug dressing (PLD-SLD), polycaprolactone (PCL)/sodium alginate (SA) was used to load platelet-derived growth factor (PDGF) and lidocaine hydrochloride (LID) by Janus electrospinning and self-assembly technology, and Ɛ-polylysine was used as a biological bacteriostatic agent to prepare a multi-layer dressing. SEM showed that the dressing had a fluffy structure. The dressing can pump the exudate to the SA layer away from the skin. The swelling ratio reached 1378.667 ± 44.752 %. Coagulate blood in 5 min. On the 8th day, the unclosed area rate of the PLD-SLD dressing group was 16.112 ± 0.088 % lower than that of the model group. Importantly, the dressing can induce the expression of CD31, VEGF, α-SMA, and reduce the expression of CD68, thereby giving priority to wound healing. There was no scar formation after healing. In this study, a new dressing preparation method was proposed for the problems of exudate management, infection control, pain relief and healing promotion of stage 3–4 pressure ulcer healing.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Beibei Ma,Yawen Liang,Xiaokai Ma,Wenjie Wang,Chengcheng Miao,Guanqi Yu,Qizhao Wang,Chunyang Xu,Xinwei Cui
The rising demand for cost-effective and safe energy storage systems has ignited strong global interest in advanced cathodes that surpass lithium-ion batteries in practical applications. Herein, we propose a composite material in which five ions, Ti 4+ , Mn 2+ , Fe 2+ , Zr 4+ , and Mo 6+ , are doped into the lattice of Na 3 V 2 (PO 4 ) 3 and a uniform carbon-coating layer is applied to its surface, resulting in the formation of high-entropy doped Na 3 V 2 (PO 4 ) 3 @C materials (HENVP@C). When employed as the cathode material for Sodium-ion batteries (SIBs) and hybrid lithium/sodium-ion batteries (HLSIBs), HENVP@C shows remarkable Li + /Na + storage capabilities. The results of electrochemical performance tests on half-cells reveal that HENVP@C exhibits a high capacity, along with excellent cycle life and stability. High-entropy doping effectively stabilizes the NASICON structure of HENVP@C, enhances ionic conductivity, and optimizes ion diffusion kinetics, particularly improving its dual-ion pseudocapacitive storage capability. In addition, the surface carbon coating improves the electronic conductivity of the material. The insights presented in this work provide new ideas for designing cathode materials for SIBs and HLSIBs.
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DOI
PubMed
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2025
·
IF 5
Lingjun Zhou,Panyi Wang,Tenglong Sun,Yanan Chen,Muzhi Cai,Wenyan Zheng,Feifei Huang,Ying Tian,Liaolin Zhang,Junjie Zhang
Tellurite glasses with the composition 67TeO 2 -3Al 2 O 3 -xZnO-(30-x)Na 2 O (x = 0–20 mol%) were synthesized via melt-quenching. Raman analysis revealed Al/Zn synergistically strengthened the network via Al-O-Te/Zn-O-Te bonds below 15 mol% ZnO, whereas excess ZnO triggered Zn 2+ /Al 3+ site competition, reducing crystallization resistance. The glass with 15 mol% ZnO (TANZ) shows optimal thermal stability ( T g = 311 °C, ΔT = 139 °C) and serves as the host for obtaining mid-infrared emission. The Ho 3+ /Yb 3+ co-doped TANZ glass shows high absorption and emission cross-sections of 7.36 × 10 –21 cm 2 and 5.86 × 10 –21 cm 2 , respectively, along with a long lifetime of 1.33 ms at 2 μm. These results highlight the potential of TANZ glasses as robust gain materials for mid-infrared fiber lasers.
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高影响因子
Food Production Processing and Nutrition
·
2025
·Vol. 7
·期 1
·1-14
·
IF 5.7
Sun Teng,Wang Zhaojun,Dai Yiqiang,Zhai Kang,Fang Xiangna,Chen Yitian,Zhao Baomin,Dong Mingsheng
In this study, Rhizopus oligosporus RT-3 was utilized to ferment soybean meal to obtain a tempe-like food with low fat and high bioactivity. To investigate the impact of fermentation on soybean meal’s nutritional value, we detected the protein-related indices and conducted a non-targeted metabonomic analysis on both fermented soybean meal (FSM) and soybean meal (SM) in order to explore changes in a certain type of metabolite and its corresponding metabolic pathways. The results showed a significant increase in crude protein, peptides and amino acids while getting porous after fermentation. A reduction of raffinose and stachyose to 18.1% and 8.5%, respectively, due to the action of α-galactosidase and β-furanofructosidase occurred. As to amino acid metabolites, essential amino acids for human bodily functions such as lysine, histidine, and threonine were significantly upregulated. Furthermore, nine peptide metabolites were identified as angiotensin-converting enzyme (ACE) inhibitors and dipeptidyl peptidase-4 inhibitors (DPP-IV). After feeding with different doses of FSM, levels of insulin-like growth factor-1 (IGF-1), hemoglobin (HB), albumin (ALB), and total protein (TP) of malnourished mice all returned to normal. In conclusion, fermentation with Rhizopus oligosporus RT-3 can improve the quality of SM by reducing the level of oligosaccharide anti-nutrition factors and increasing the contents of free amino acid and small bioactive peptides, which provide a theoretical basis and new ideas for the application of FSM. Graphical
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危险品化学品经营许可证（带存储）