WATER AIR AND SOIL POLLUTION
·
2016
·Vol. 228
·期 1
·1-12
·
IF 1.551
Jiang Meng,Qi Yun,Cui Yuan-Lu,Zhao Lin,Liu Shejiang
A rutin-Cr(III) loaded alginate microspheres, which can act as reduction-adsorption microreactor, was designed to reduce Cr(VI) to Cr(III) and recover it. Different from the rutin-Cr(III) complex in alginate-chitosan microcapsule we previously reported, which can sustained release rutin-Cr(III) and reduce Cr(VI) to Cr(III), this microreactor can not only reduce Cr(VI) but also adsorb/recover Cr(III) in aqueous solution. This microreactor was prepared by loading metal complex of rutin into alginate microsphere. It was found that Cr(VI) was initially reduced to Cr(III) by rutin-Cr(III), and then the product Cr(III) was absorbed in the microspheres. Both Cr(VI) reduction rate and Cr(III) adsorption rate were pH-dependent. Cr(VI) can be completely reduced to Cr(III) at pH 1–4 with a reduction capability of 1.27 (±0.078) mmol/g (pH 1, 4 h). The highest blank alginate microsphere adsorption capacity was 1.82 (±0.042) mmol/g at pH 4. It was assumed that reduction-adsorption was the mechanism of total Cr removal by the reduction-absorption microreactor. Recovery experiment showed that 93% and 98% of Cr(III) can be recovered by EDTA and sodium citrate, respectively.
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MOLECULAR NEUROBIOLOGY
·
2016
·Vol. 55
·期 1
·421-434
·
IF 5.397
Dai Chongshan,Ciccotosto Giuseppe D.,Cappai Roberto,Tang Shusheng,Li Daowen,Xie Sanlei,Xiao Xilong,Velkov Tony
Neurotoxicity is an unwanted side-effect seen in patients receiving therapy with the “last-line” polymyxin antibiotics. This is the first study to show that colistin-induced neurotoxicity in neuroblastoma-2a (N2a) cells gives rise to an inflammatory response involving the IL-1β/p-IκB-α/NF-κB pathway. Pretreatment with curcumin at 5, 10, and 20 μM for 2 h prior to colistin (200 μM) exposure for 24 h, produced an anti-inflammatory effect by significantly down-regulating the expression of the pro-inflammatory mediators cyclooxygenase-2 (COX-2), phosphorylation of the inhibitor of nuclear factor-kappa B (NF-κB) (p-IκB)-α, and concomitantly NF-κB levels. Moreover, curcumin significantly decreased intracellular reactive oxygen species (ROS) production and increased the activities of the anti-ROS enzymes superoxide dismutase, catalase, and the intracellular levels of glutathione. Curcumin pretreatment also protected the cells from colistin-induced mitochondrial dysfunction, caspase activation, and subsequent apoptosis. Overall, our findings demonstrate for the first time, a potential role for curcumin for treating polymyxin-induced neurotoxicity through the modulation of NF-κB signaling and its potent anti-oxidative and anti-apoptotic effects.
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PubMed
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JOURNAL OF NANOPARTICLE RESEARCH
·
2016
·Vol. 18
·期 12
·1-16
·
IF 2.101
Li Zhenzhen,Zhang Qiyi,Huang Huaying,Ren Changjing,Pan Yujin,Wang Qing,Zhao Qiang
In the experiments, high-quality, water-soluble and near-infrared (NIR)-emitting CdSeTe and CdSeTe/CdS quantum dots (QDs) were successfully prepared. The average size of CdSeTe⁄CdS QDs was 7.68 nm and CdSeTe QDs was 4.33 nm. Arginine-glycine-aspartic-serine acid (RGDS) peptides were linked to CdSeTe/CdS QDs by N-(3-(dimethylamino)propyl)-N′-ehtylcarbodiimide hydrochloride (EDC) and N′-hydroxysuccinimide (NHS). The prepared RGDS-tagged NIR CdSeTe/CdS QDs (denoted as RGDS-CdSeTe/CdS) had an average diameter of 24.83 nm and were used for cancer cell immunofluorescence imaging. The characteristics of RGDS-conjugated CdSeTe/CdS such as morphology, structure, spectra, stability, cytotoxicity, and near-infrared microscopic imaging were investigated in detail. HepG2 cells were incubated with the novel fluorescent probe (RGDS-CdSeTe/CdS), which realized immunofluorescence targeting and imaging. The results reported here open up new perspectives for integrin-targeted near-infrared imaging and may aid in tumor detection including imaging-guided surgery.
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MICROCHIMICA ACTA
·
2016
·Vol. 184
·期 2
·547-555
·
IF 4.831
Zhang Lei,Gan Yangyang,Sun Haofan,Yu Bohao,Jin Xiaofeng,Zhang Runsheng,Zhang Weibing,Zhang Lingyi
Magnetic mesoporous carbon composites incorporating hydrophilic metallic nanoparticles were synthesized from resol, ZrO(NO 3 ) 2 , ferric acetylacetonate, and triblock copolymer F127. The method involves a multi-component co-assembly strategy associated with direct carbonization. The resulting carbon material is shown to be useful as a metal oxide affinity chromatography (MOAC) material for enrichment of phosphopeptides owing to its large mesoporous (4.8 nm) surface area (442 m 2 g −1 ), large pore volume (0.37 cm 3 g −1 ) and excellent hydrophilicity. The metallic iron and ferric oxide particles modified on the mesoporous carbon exert a magnetic force and, in combination with the metallic zirconia, is a viable MOAC material for enrichment of low-abundance phosphopeptides. Because of metal chelation between metallic nanoparticles and the phosphate groups of phosphopeptides, the zirconia/magnetic mesoporous carbon displays high selectivity even at a phosphopeptide/nonphosphopeptide molar ratio of 1:500. As little as 1.5 fmol of phosphopeptides become detectable. The MOAC was successfully applied to the identification by MALDI-TOF MS of phosphopeptides in human serum and nonfat milk.
Magnetic mesoporous carbon composites incorporating hydrophilic metallic nanoparticles were prepared and successfully applied to the enrichment of low-abundance phosphopeptides from human serum and nonfat milk by MALDI-TOF mass spectrometry.
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JOURNAL OF POROUS MATERIALS
·
2016
·Vol. 24
·期 3
·613-620
·
IF 1.385
Fu Yuanyuan,Zhong Baoying,Chen Yaqin,Song Yonggui,Zhou Rihui,Song Yonghai,Chen Shouhui
A novel porous spherical ZnO@carbon (C) nanocomposite based on zeolitic imidazolate frameworks (ZIFs-8)-directed method was prepared for lithium-ion batteries (LIBs). In this strategy, spherical ZnO nanoparticles were firstly prepared, then 2-methylimidazolate and Zn 2+ were added alternately under ultrasound to fabricate ZnO@ZIF-8. Finally, the novel porous spherical ZnO@C nanocomposites were obtained via pyrolyzing the corresponding ZnO@ZIF-8. The novel porous spherical ZnO@C nanocomposites were characterized with different analysis techniques such as scanning electron microscopy, transmission electron microscopy and X-ray powder diffraction. The resulted spherical ZnO@C nanocomposites exhibited a high reversible capacity of 932 mA h g −1 at 0.1 A g −1 after 100 cycles, which is much higher than that of the pure ZnO nanoparticles. The porous structure, high specific surface area and good electrical conductivity eventually contribute to the good performance of the resulted ZnO@C nanocomposites for LIBs should be ascribed to the proous structure and high BET surface area derived from ZIFs, as well as the good electrical conductivity of the amourphous carbon derived from ZIFs.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2016
·
IF 2.101
Zhang Jing,Li Mengfei,Yuan Zhefan,Wu Dan,Chen Jia-da,Feng Jie
Abstract A novel type of stepwise-activable multifunctional peptide-guided prodrug micelles (MPPM) was fabricated for cancerous cells intracellular drug release. Deca-lysine sequence (K 10 ), a type of cell-penetrating peptide, was synthesized and terminated with azido-glycine. Then a new kind of molecule, alkyne modified doxorubicin (DOX) connecting through disulfide bond (DOX-SS-alkyne), was synthesized. After coupling via Cu-catalyzed azide–alkyne cycloaddition (CuAAC) click chemistry reaction, reduction-sensitive peptide-guided prodrug was obtained. Due to the amphiphilic property of the prodrug, it can assemble to form micelles. To prevent the nanocarriers from unspecific cellular uptake, the prodrug micelles were subsequently modified with 2,3-dimethyl maleic anhydride to obtain MPPM with a negatively charged outer shell. In vitro studies showed that MPPM could be shielded from cells under psychological environment. However, when arriving at mild acidic tumor site, the cell-penetrating capacity of MPPM would be activated by charge reversal of the micelles via hydrolysis of acid-labile β -carboxylic amides and regeneration of K 10 , which enabled efficient internalization of MPPM by tumor cells as well as following glutathione- and protease-induced drug release inside the cancerous cells. Furthermore, since the guide peptide sequences can be accurately designed and synthesized, it can be easily changed for various functions, such as targeting peptide, apoptotic peptide, even aptamers, only need to be terminated with azido-glycine. This method can be used as a template for reduction-sensitive peptide-guided prodrug for cancer therapy. Graphical abstract A novel type of stepwise-activable multifunctional peptide-guided prodrug micelles (MPPM) was fabricated for selective drug delivery in cancerous cells. MPPM could be shielded from cells under psychological environment. However, when arriving at mild acidic tumor site, the cell-penetrating capacity of MPPM would be activated by charge reversal of the prodrug micelles via hydrolysis of acid-labile β -carboxylic amides and regeneration of K 10 . The cell-penetrating peptide, K 10 , would enable efficient internalization of MPPM by tumor cells, and the drug would be rapidly released induced by concentrated glutathione and numerous proteases inside the cancerous cells, resulting in the inhibited effect of the cells .
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CELLULOSE
·
2015
·Vol. 22
·期 4
·2365-2374
·
IF 3.573
Ge Wenjiao,Guo Yanzhu,Zhong Haoquan,Wang Xiaohui,Sun Runcang
In response to the shortage of petroleum resources and the growing need for sustainable development, cellulose-based amphiphilic copolymers have emerged as a new generation of value-added functional nanostructures from biomass resources. In this article, 17 amphiphilic hydroxyethyl cellulose-based graft copolymers with different side chains, including poly(lactide), poly( ε -caprolactone) and poly( p -dioxanone), were synthesized via homogeneous ring opening polymerization in ionic liquid 1-butyl-3-methylimidazolium chloride and characterized by FT-IR, 1 H NMR, thermogravimetric analysis and gel permeation chromatography. The resultant copolymers can self-assemble into micelles with a low critical micelle concentration that varies in the range of 0.03–0.24 mg/ml. TEM observations revealed the obtained micelles had a spherical and well-distributed morphology, and DLS analysis showed the nanoscaled sizes were between 40 and 150 nm. These HEC-based micelles can be used as nano-sized vesicles and have great latent forces in drug delivery systems.
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Food Analytical Methods
·
2015
·Vol. 9
·期 3
·699-705
·
IF 1.956
Yue Mei-E,Li Qian,Xu Jie,Jiang Ting-Fu
A rapid, simple, and sensitive method has been developed for the analysis of triazine herbicides in honey samples by using salt de-emulsification dispersive liquid-liquid microextraction and back-extraction (SD-DLLME-BE) coupled with sweeping in micellar electrokinetic chromatography (MEKC). In the newly developed method, chloroform can be generated by the reaction of chloral hydrate and sodium hydroxide, and the chloroform droplets dispersed into bulk aqueous solution can be swiftly carried to the bottom of the centrifuge tube with the addition of calcium chloride. Then, back-extraction was utilized to extract the analytes into an aqueous solution prior to capillary electrophoresis (CE) analysis. The advantages of the new in situ DLLME lie in dispersive solvent-free and no centrifugation operation compared with the conventional DLLME. Several important parameters influencing the preconcentration and extraction efficiency of SD-DLLME-BE such as sodium dodecyl sulfate (SDS) concentration, pH, chloral hydrate and sodium hydroxide content, extraction time, and calcium chloride content were optimized. Under the optimized conditions, the enrichment factors were achieved in the range from 459 to 636. The proposed method provided a good linearity, low limits of detection (8.56 ng/mL), and good repeatability of the extractions (relative standard deviations (RSDs) below 8.8 %, n = 6). Honey samples were analyzed by the proposed method, and obtained results indicated that the proposed method provides acceptable recoveries and precisions.
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FIBERS AND POLYMERS
·
2014
·Vol. 15
·期 8
·1609-1615
·
IF 1.113
Yu Yuanyuan,Yuan Jiugang,Wang Qiang,Fan Xuerong,Wang Ping,Cui Li
Our previous studies have demonstrated that cotton fabrics treated with soluble immobilized-cellulase showed considerably lower degradation and higher retention of tensile strength than those treated with free cellulase. It is important to investigate the surface morphology and structure of cotton fibres for understanding the enzymatic degradation. In this study, the effects of the soluble immobilized-cellulase on the surface morphology and structure of cotton fibres were investigated. The ultrastructural changes in the fibre surfaces were inspected using Tapping-Mode Atomic Force Microscopy (TM-AFM) and scanning electron microscopy (SEM), and the results showed that the smooth surfaces could be obtained after the immobilized-cellulase treatment, and no obvious damage was observed on the fibre surfaces. The hydrogen bonds in the certain depth area beneath the fibre surface were investigated using Attenuated Total Reflectance Fourier-Transform Infrared Spectroscopy (ATR-FTIR) after the cellulase treatments. Furthermore, the result of fibre accessibility indicated that the hydrolysis occurring in the interior of the cotton fibres was limited during the immobilized-cellulase treatment. Crystalline index (CI) of the cotton fibres treated with free cellulase was slightly higher than that of fibres treated with immobilized cellulase.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2013
·Vol. 15
·期 4
·1-9
·
IF 2.175
Jia Li-Ping,Yan Bing,Zhang Qiang
A high temperature solution system with BaCO 3 /RECl 3 ·xH 2 O in oleic acid/1-octadecene has been developed to prepare barium rare earth fluoride nanocrystals: tetragonal phase of BaREF 5 (RE = Pr, Nd, Eu, Tb, Dy, Tm, Lu, Y), pure cubic phase Ba 2 REF 7 (RE = La, Sm, Ho, Er, Yb), and BaREF 5 (RE = Ce, Gd) colloidal nanocrystals. In addition, it is proved that the functionalization of these nanocrystals by means of doping with Eu, Tb, and Yb, Er/Tm ion pair can obtain red, green, and visible-to-the-naked-eye green/blue upconversion emissions.
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CHEMICAL PAPERS
·
2013
·Vol. 67
·期 5
·517-525
·
IF 0.879
Wu Xing,Yi Jian-Min,Liu Yue-Jin,Liu Yong-Bing,Zhang Pan-Liang
The rapid expansion of a supercritical solution (RESS) process represents an attractive prospect for producing sub-micron and nano-particles of medical compounds with low solubility. The solubility of phenacetin in supercritical carbon dioxide was measured by the analytical-isothermal method at pressures ranging from 9.0 MPa to 30.0 MPa and temperatures ranging from 308.0 K to 328.0 K. The results show that the mole fraction solubility of phenacetin in supercritical carbon dioxide is up to 10 −5 . Four density-based semi-empirical models were introduced to correlate the experimental data. Agreement between the model predictions and experimental data is greater with the Adachi-Lu-modified Chrastil model than with the Chrastil model, Méndez-Santiago-Teja model, and the Bartle model and the average absolute relative deviation (AARD) observed is 0.0483. The preparation of fine phenacetin particles by the RESS process under different conditions of extraction temperatures (308.0–328.0 K), extraction pressures (9.0–30.0 MPa), nozzle temperatures (373.0–393.0 K), nozzle diameters (0.1–0.8 mm), and collection distance (20.0–40.0 mm) was investigated. The size and morphology of the resultant particles were analysed by SEM. A remarkable modification in size and morphology can be obtained by condition-optimisation.
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Chemistry Central Journal
·
2012
·Vol. 6
·期 1
·1-10
·
IF 3.281
Li Xican,Lin Jing,Gao Yaoxiang,Han Weijuan,Chen Dongfeng
Background As a typical Chinese herbal medicine, rhizoma Cimicifugae (RC, 升麻 in Chinese) possesses various pharmacological effects involved in antioxidant activity. However, its antioxidant activity has not been reported so far. The aim of the present study was to systematically evaluate the antioxidant ability of RC in vitro, then discuss the mechanism. Methods Firstly, five RC extracts (i.e. petroleum ether extract PERC, ethyl acetate extract EARC, absolute ethanol extract AERC, 95% ethanol extract 95ERC, and water extract WRC) were prepared and determined by various antioxidant methods, including anti-lipidperoxidation, protection against DNA damage, ·OH scavenging, ·O 2 - scavenging, DPPH· (1,1-diphenyl-2-picryl-hydrazl radical) scavenging, ABTS + · (2,2’-azino-bis (3-ethylbenzo- thiazoline-6-sulfonic acid radical ion) scavenging, Cu 2+ -chelating, and Fe 3+ reducing assays. Subsequently, we measured the chemical contents of five RC extracts, including total phenolics, total saponins, total sugars, caffeic acid, ferulic acid and isoferulic acid. Finally, we quantitatively analyzed the correlations between antioxidant levels (1/IC 50 values) and chemical contents. Results In the study, the antioxidant levels and chemical contents (including total phenolics, total saponins, total sugars, caffeic acid, ferulic acid and isoferulic acid) of five RC extracts were determined by various methods. In all antioxidant assays, five RC extracts increased the antioxidant levels in a dose-dependent manner. However, their antioxidant levels (IC 50 values) and chemical contents significantly differed from each other. Quantitative analysis of the correlation showed that total phenolic was of significant positive correlations (average R value was 0.56) with antioxidant levels; In contrast, total sugars and total saponins had no positive correlation with antioxidant (the average R values were −0.20 and −0.26, for total sugars and total saponins, respectively); Among total phenolics, three phenolic acids (caffeic acid, ferulic acid and isoferulic acid) also displayed positive correlations (the average R values were 0.51, 0.50, and 0.51, for caffeic acid, ferulic acid and isoferulic acid, respectively). Conclusions As an effective antioxidant, Rhizoma Cimicifugae can protect DNA and lipids against oxidative damage. Its antioxidant ability can be responsible for its various pharmacological effects and may be mainly attributed to the existence of total phenolics, among which caffeic acid, ferulic acid and isoferulic acid are regarded as main bioactive components. Rhizoma Cimicifugae exerts its antioxidant effect through metal-chelating, and radical-scavenging which is via donating hydrogen atom (H·) and donating electron (e).
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