Frontiers in Immunology
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2022
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IF 8.786
Wei Shujin,Jiao Dian,Xing Wanli
Both Gram-negative and Gram-positive bacteria can release vesicle-like structures referred to as bacterial extracellular vesicles (BEVs), which contain various bioactive compounds. BEVs play important roles in the microbial community interactions and host-microbe interactions. Markedly, BEVs can be delivered to host cells, thus modulating the development and function of the innate immune system. To clarify the compositions and biological functions of BEVs, we need to collect these vesicles with high purity and bioactivity. Here we propose an isolation strategy based on a broad-spectrum antimicrobial epsilon-poly-L-lysine (ϵ-PL) to precipitate BEVs at a relatively low centrifugal speed (10,000 × g). Compared to the standard ultracentrifugation strategy, our method can enrich BEVs from large volumes of media inexpensively and rapidly. The precipitated BEVs can be recovered by adjusting the pH and ionic strength of the media, followed by an ultrafiltration step to remove ϵ-PL and achieve buffer exchange. The morphology, size, and protein composition of the ϵ-PL-precipitated BEVs are comparable to those purified by ultracentrifugation. Moreover, ϵ-PL-precipitated BEVs retained the biological activity as observed by confocal microscopy studies. And THP-1 cells stimulated with these BEVs undergo marked reprogramming of their transcriptome. KEGG analysis of the differentially expressed genes showed that the signal pathways of cellular inflammatory response were significantly activated. Taken together, we provide a new method to rapidly enrich BEVs with high purity and bioactivity, which has the potential to be applied to BEVs-related immune response studies.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2021
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IF 4.539
Jiaojiao Cao,Yubo Lian,Huatao Weng,Zhenyu Chen
The effect of an external DC electric field on the inhibition behavior of Zn-MOFs in 0.5 mol/L NaCl solution for copper was investigated via electrochemical measurements and various characterization techniques. Results indicated that the corrosion inhibition efficiency of the Zn-MOFs decreased under strong electric field intensities, which went from 94.2% to 61.7%. The corrosion inhibition efficiency of the materials decreased with increasing electric field duration when maintained at a certain electric field intensity. When the electric field duration time was 6 h, 12 h, 18 h and 24 h, the corresponding efficiency was 90.4%, 86.8%, 78.2% and 61.7% respectively. The effect of an electric field on the corrosion inhibition performance of the Zn-MOFs could essentially be attributed to changes in the structure of the material framework.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2021
·Vol. 45
·期 2
·353-364
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IF 3.21
Li Xue-Yong,Xu Meng-Qiu,Liu Hui,Zhou Qiang,Gao Jian,Zhang Ye-Wang
The combined cross-linked enzyme aggregates (combi-CLEAs) containing galactitol dehydrogenase (Gdh) and NADH oxidase (Nox) were prepared for l -tagatose synthesis. To prevent the excess consumption of cofactor, Nox in the combi-CLEAs was used to in situ regenerate NAD + . In the immobilization process, ammonia sulfate and glutaraldehyde were used as the precipitant and cross-linking reagent, respectively. The preparation conditions were optimized as follows: 60% ammonium sulfate, 1:1 (molar ratio) of Gdh to Nox, 20:1 (molar ratio) of protein to glutaraldehyde, and 6 h of cross-linking time at 35 °C. Under these conditions, the activity of the combi-CLEAs was 210 U g −1 . The combi-CLEAs exhibited higher thermostability and preserved 51.5% of the original activity after eight cycles of reuses at 45 °C. The combi-CLEAs were utilized for the preparation of l -tagatose without by-products. Therefore, the combi-CLEAs have the industrial potential for the bioconversion of galactitol to l -tagatose. Graphical abstract
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Yaoyao Xu,Kehong Hou,Chengcheng Gao,Xiao Feng,Weiwei Cheng,Di Wu,Linghan Meng,Yuling Yang,Xinchun Shen,Yan Zhang,Xiaozhi Tang
To improve the antimicrobial properties of chitosan films, cinnamon essential oil (CEO) nanoemulsion (1% and 3% v/v CEO) stabilized by ethyl-N α -lauroyl- l -arginate hydrochloride (LAE) alone or co-stabilized by LAE and hydroxypropyl-β-cyclodextrin (HPCD) were incorporated into chitosan matrix. The micromorphology , physical and antimicrobial properties of the composite films were compared. The dense structure of the CEO nanoemulsion co-stabilized by LAE and HPCD reduced the water vapor permeability and water content. The incorporation of the CEO nanoemulsion co-stabilized by LAE and HPCD, reduced the adverse effects of CEO on the mechanical properties and microstructure of the film, and even slightly increased the tensile strength . In addition, the antimicrobial properties of chitosan films were enhanced due to the encapsulation and emulsification effect of HPCD and LAE on CEO. This work indicated that the prepared chitosan based edible films had the potential to be used in the field of food packaging to improve food safety.
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CHINESE JOURNAL OF POLYMER SCIENCE
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2021
·Vol. 39
·期 8
·966-974
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IF 3.603
Feng Li-Dong,Bian Xin-Chao,Li Gao,Chen Xue-Si
The effect of exogenous hydroxyl, carboxyl groups and/or Sn 2+ on pyrolysis reactions of poly(L-lactide) (PLLA) was investigated by thermogravimetric analysis (TGA). The activation energy ( E a ) of pyrolysis reactions was estimated by the Kissinger-Akahira-Sunose method. The kinetic models were also explored by the Malek method, and the random degradation behavior was determined by comparing the plots of In{-In[1 - (1 - w ) 0.5 ]} versus 1/ T for experimental data from TGA with model reactions. The pyrolysis reaction rate of PLLA was affected slightly by exogenous hydroxyl and carboxyl groups at lower levels of Sn with 65–70 mg·kg -1 but increased appreciably in the presence of extraneous Sn 2+ , -COOH/Sn 2+ , or -OH/Sn 2+ . The E a values for the pyrolysis reactions of the PLLAs that provided lactide were different under the catalysis of Sn 2+ in different chemical environments because Sn 2+ can form the new Sn-carboxylate and Sn-alkoxide with exogenous carboxyl and hydroxyl groups, which were different in steric hindrance for the formation of activated complex between Sn 2+ and PLLA. Under the catalysis of Sn 2+ , a lactide molecule can be directly eliminated selectively at a random position of PLLA molecular chains, and the molecular chain of PLLA cannot change two PLLA fragments at the elimination site of lactide. However, it was regenerated into a new PLLA molecule with the molecular weight reduced by 144 g·mol -1 .
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 413
·期 15
·4013-4022
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IF 4.157
Liu Chao,Zhao Yunmeng,Xu Di,Zheng Xinxin,Huang Qing
A facile and green approach to the preparation of peroxidase-like nanozymes by reducing and functionalizing graphene oxide (rGO) with Ganoderma polysaccharide (GP) has been achieved in this work. Our results showed that the as-fabricated nanozyme, namely rGO-GP, possessed the excellent property of simulating peroxidase with higher catalytic activity compared with GO or rGO obtained by using chitosan, which may be due to the better dispersion of rGO-GP in the solution. Steady-state kinetics studies further showed that the catalytic process conformed to Michaelis-Menten equation and ping-pong mechanism. Benefiting from the excellent peroxidase property of rGO-GP, we have also successfully established a highly sensitive and selective colorimetric detection approach to trace detection of l -cysteine ( l -Cys). The limit of detection (LOD) of l -cysteine is 0.1 μM and the linear detection range is 2–30 μM. Furthermore, the nanozyme was successfully applied for detecting l -cysteine in serum. This work therefore demonstrates the advantages of rGO-GP as an effective nanozyme in both its green synthesis and detecting application.
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JOURNAL OF MOLECULAR LIQUIDS
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2021
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IF 6.165
Fukang Deng,Kangchun Li,Qing Feng,Kedi Yang,Fuchuan Huang
As the life expectancy increases, so do the wear and damage to joints caused by increased physical activity. In this regard, the development of biofriendly and nontoxic lubricating materials has important social and economic impact. Therefore, this work develops such a lubricant for joints using tea saponin derivative (TSC16) as the bio-lubricating additive to a choline/N-acetyl- l -proline ionic liquid (IL). The TSC16 molecules aggregated in the choline/N-acetyl- l -proline IL to form short fibrous structures, which increased the viscosity of the IL and improved the formation of a lubricating film on the applied area. The fibrous structure of TSC16 enhanced the strength of the lubricating film and improved the lubrication and anti-wear performance. Overall, the developed IL blend exhibits promise for tissue engineering applications.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
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IF 4.952
Jia-Nan Chen,Xu-Hui Huang,Jie Zheng,Yi-Han Sun,Xiu-Ping Dong,Da-Yong Zhou,Bei-Wei Zhu,Lei Qin
Blue mussel ( Mytilus edulis L.) is a shellfish with high nutritional and economic value. Free amino acids (FAAs), 5′-nucleotides, and lipids that affected the nutritional value and flavor of blue mussels varying with the seasons were analyzed using stable isotope labeling-liquid chromatography coupled with tandem mass spectrometry (SIL-LC-MS/MS) and ultra-performance liquid chromatography-Q-Exactive orbitrap tandem mass spectrometry (UPLC-Q-Exactive Orbitrap-MS). FAAs and 5′-nucleotides were most in the summer. Changes in FAAs between seasons were more obvious than those between genders, except in spring, when females were in the reproductive period. A total of 413 lipids were annotated in blue mussels for one year. Seasonal variation in triacylglycerols (TAGs) was the most evident. TAGs were dominant in females, while phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs) were dominant in males. Overall, blue mussels in summer were more delicious and nutritious. Thus, such findings are of great significance to the seasonal preference of deep processing and consumption of mussel products.
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ANALYTICA CHIMICA ACTA
·
2020
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IF 5.977
Zihan Wang,Li Zhang,Yumin Hao,Wenjuan Dong,Yang Liu,Shengmei Song,Shaomin Shuang,Chuan Dong,Xiaojuan Gong
The fluorescent sensor, especially ratiometric fluorescent sensor, is one of the most important applications for CQDs , which is becoming a research hotspot. Herein, carbon quantum dots co-doped with nitrogen, phosphorus and chlorine (NPCl-CQDs) were synthesized by acid-base neutralization reaction exothermic carbonization method. The as-fabricated NPCl-CQDs could emit blue fluorescence and possess excellent fluorescence properties. Based on the FRET , multifunctional and ratiometric fluorescent sensors for “on-off-on” sequential determination of riboflavin , Ag + , and Cys with good selectivity and high sensitivity were established. The linear range of riboflavin, Ag + , and Cys are 0.50–10.18 μM and 15.89–27.76 μM, 0.66–1.46 mM and 1.50–4.20 mM, and 0.01–0.15 μM and 0.15–0.36 μM with the limit of detection of 3.50 nM, 26.38 μM, and 0.96 nM, respectively. Furthermore, the sensors were successfully used to determine riboflavin, Ag + , and Cys in tablets, river water, and human urine with the recoveries of 95.2–104.0%, 95.6–102.0%, and 94.8–106.4%, respectively. More importantly, the as-constructed “on-off-on” NPCl-CQDs-based ratiometric fluorescent sensors were applied for detecting riboflavin, Ag + , and Cys in HeLa cells with satisfying results. The finding of this study shows the feasibility and effectiveness of the NPCl-CQDs as the available ratiometric fluorescent sensors for the determination of riboflavin, Ag + , and Cys in real samples and living cells.
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FOOD RESEARCH INTERNATIONAL
·
2020
·
IF 4.972
Halah Aalim,Di Wang,Zisheng Luo
Black rice is recognized for managing diabetes in Chinese folk medicine. Therefore, the present study investigates the effect of thermal treatments and the succeeding cooking on black rice physicochemical properties, phenolic composition, total antioxidant activity (TAA), enzymes and glycation inhibition in addition to starch digestibility. Thermal decomposition of anthocyanin and cyanidin-3-glucoside was evident across all processing methods and reflected in increasing levels of protocatechuic acid, while proanthocyanidins (TPAC) were susceptible to cooking. Roasting of grains sustained total phenolics (TPC), flavonoids (TFC), TPAC, and antilipase activity. Additionally, the combined effect of frying and cooking diminished TFC, TPAC, and α-glucosidase inhibition. The thermally treated grains showed pronounced activity against α-amylase, α-glucosidase, and glycation, whereas their cooked counterparts reduced the estimated glycemic index (eGI), and enhanced resistant starch (RS). Processed grains chrominance, TAA, and apparent amylose content (AAC) showed a significant correlation with phenolics. These findings are demonstrating that black rice processing is favorable for the dietary management of metabolic disorders such as diabetes and hyperlipidemia.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2020
·
IF 4.939
Hongmei Zhao,Tingting Zhang,Dongyu Shan,Ying Zhu,Ge Gao,Ye Liu,Jing Liu,Meiying Liu,Wansheng You
An intervening barrier for photocatalytic water decomposition and pollutant degradation is the frustratingly quick recombination of e − - h + pairs. Delicate design of heterojunction photocatalysts by coupling the semiconductors at nanoscale with well-matched geometrical and electronic alignments is an effective strategy to ameliorate the charge separation. Here a facile and environment-friendly l -cysteine-assisted hydrothermal process under weakly alkaline conditions is demonstrated for the first time to fabricate ZnIn 2 S 4 /In(OH) 3 hollow microspheres with intimate contact, which are verified by XRD, SEM, (HR)TEM, XPS , N 2 adsorption-desorption, UV–Vis DRS and photoluminescence spectra. ZnIn 2 S 4 /In(OH) 3 heterostructure (L-cys/Zn 2+ = 4, molar ratio) with a band-gap of 2.50 eV, demonstrates the best photocatalytic performance for water reduction and MB degradation under visible light, outperforming its counterparts (In(OH) 3 and ZnIn 2 S 4 ). The excellent activity of ZnIn 2 S 4 /In(OH) 3 heterostructure arises from the intercrossed band-edge positions as well as the unique hollow structure with large surface area and wide pore-size distribution, which are beneficial for the efficient charge migration from bulk to surface as well as at the interface between ZnIn 2 S 4 and In(OH) 3 . This work provides an efficient and eco-friendly strategy for one-pot synthesis of heterostructured composites with intimate contact for photocatalytic application.
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Journal of Functional Foods
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2020
·
IF 3.701
Fenfen Li,Danfei Huang,Weiyu Yang,Xiaozhen Liu,Shaoping Nie,Mingyong Xie
The preventive role of polysaccharide from the seeds of Plantago asiatica L. (PLCP) against reproductive system injury induced by nonylphenol (NP) was investigated. Male Sprague-Dawley rats were exposed orally to NP 1 h after PLCP intragastric administration continuously for 45 days. The results showed that NP induced significant decrease in relative weight of testes and epididymis, changed testicular histomorphology, and disrupted hormone secretions. Moreover, oxidative stress occurred after NP exposure by overproduction of Malondialdehyde, lipid peroxidation, inducible nitric oxide synthase, and low activities of superoxide dismutase and catalase. Furthermore, the induction of apoptosis and disturbance of PI3K/Akt/mTOR pathway by NP were also proved. Interestingly, PLCP alleviated NP toxicity by improving antioxidant enzymes, recovering hormone secretions, inhibiting apoptosis and autophagy, which involves PI3K/Akt/mTOR pathway. These findings provide a good direction that PLCP may be a good candidate to help body defend against toxic substance, particularly endocrine disrupting chemicals induced reproductive toxicity.
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