JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 12
·2565-2574
·
IF 2.557
Senlin Li,Sainan Li,Yu Huang,Chunming Liu,Lina Chen,Yuchi Zhang
The separation of a compound of interest from its structurally similar homologues to produce high-purity natural products is a challenging problem. This work proposes a novel method for the separation of iristectorigenin A from its structurally similar homologues by ionic-liquid-based ultrasound-assisted extraction and the subsequent screening and isolation of potential α-glucosidase inhibitors via ultrafiltration and semipreparative high-performance liquid chromatography. Ionic-liquid-based ultrasound-assisted extraction was successfully applied to the extraction of tectorigenin, iristectorigenin A, irigenin, and irisflorentin from Belamcanda chinensis . The optimum conditions for the efficient extraction of isoflavones were determined as 1.0 M 1-ethyl-3-methylimidazolium tetrafluoroborate with extraction time of 30 min and a solvent to solid ratio of 30 mL/g. Ultrafiltration with liquid chromatography and mass spectrometry was applied to screen and identify α-glucosidase inhibitors from B. chinensis , followed by the application of semipreparative high-performance liquid chromatography to separate and isolate the active constituents. Four major compounds including tectorigenin, iristectorigenin A, irigenin, and irisflorentin were screened and identified as α-glucosidase inhibitors, and then the four active compounds abovementioned were subsequently isolated by semipreparative high-performance liquid chromatography (99.89, 88.97, 99.79, and 99.97% purity, respectively). The results demonstrate that ionic liquid extraction can be successfully applied to the extraction of isoflavones from B. chinensis .
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 78
·74903-74912
·
IF 3.289
Xiaoyan Li,Zhengzhou Wang,Lixin Wu,Tsungyen Tsai
A novel α-zirconium phosphate/graphene oxide hybrid (ZGO) with α-zirconium phosphate (α-ZrP) nanoparticles immobilized on the surface of graphene oxide (GO) sheets was successfully synthesized by a one-step in situ method, and characterized by X-ray diffraction (XRD), thermogravimetric analysis (TGA), FTIR spectroscopy and transmission electronic microscopy (TEM). TGA results showed that the modification of α-ZrP nanoparticles on the GO surface significantly improved the thermal stability of the GO. Subsequently, the ZGO was incorporated into phenolic (PF) foams for reducing their high brittleness and friability. At a ZGO dosage of 4 phr (parts per hundreds of resin), the flexural and compressive strengths of the ZGO-toughened PF foams increased by 33.8% and by 39.6%, respectively, and the pulverization ratio decreased by 57.5%, compared with the pure PF foam. Besides, the results of the TGA test, limiting oxygen index (LOI) and cone calorimetric test demonstrated that the addition of ZGO imparted excellent thermal stability and flame retardancy to the PF foam, which was mainly attributed to the synergistic effects between α-ZrP nanoparticles and GO.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Gang Wang,Xiao-Na Xu,Zhu Zhi-Min,Kun Wang,Fei Li
This study tried to develop the α-Hederin/Oxaliplatin (OXA) dual-loaded rHDL (α-Hederin-OXA-rHDL) modified liposomes to improve the therapeutic index on colon adenocarcinoma (COAD). The α-Hederin-OXA-rHDL were prepared and evaluated for characterizations, accumulate to tumor tissues, and antitumor activity. A thorough investigation into oxaliplatin resistant and KRAS-mutant related hub keg genes were identified and performed to assess the prognosis role of the genetic signature in COAD. The potential immune signatures and molecular docking for verifing the predicted targets of α-Hederin-OXA-rHDL in tumor-bearing mice. Results suggested that α-Hederin-OXA-rHDL could enhance the sensitivity of oxaliplatin in HCT116/L-OHP cells via the regulation of KEAP1/NRF2 -mediated signaling and HO1 or GPX4 proteins. Furthermore, α-Hederin-OXA-rHDL regulated the predicted targets of PRDM1 interaction with miR-140-5p, efficient activing CD8 T cell to improve therapeutic response in vivo . Collectively, this work provides drug delivery with rHDL dual-loaded α-Hederin and oxaliplatin synergistically targets cancer cells and effectory T cells combating COAD.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Guangyi Zhang,Chengqiang Han,Yuxuan Li,Xi-Ling Li,Hongmei Jin,Jun Zhe Min
α-Dicarbonyl compounds (α-DCs) in human saliva are closely associated with diabetes, but their inherent instability presents challenges for sample transportation and detection. This study introduces a novel approach for the simultaneous detection of three α-DCs—3-deoxyglucoside (3-DG), glyoxal (GO), and methylglyoxal (MG)—in dried saliva spots using 4,5-dimethyl-1,2-phenylenediamine (DMPD) derivatization combined with UHPLC-MS/MS. 5.0 mm diameter double-circle qualitative filter paper was selected as the substrate for dried saliva, with optimal extraction conditions involving two 3-minute extractions. Chromatographic separation was performed on a CSH™ C18 column (2.1 × 50 mm, 1.7 μm) via gradient elution, utilizing a mobile phase consisting of 0.1 % formic acid in water and 0.1 % formic acid in acetonitrile. The entire separation process was completed within a 5.0 min timeframe. The linearity of 3-DG and GO in the concentration range of 0.10–12.5 μM, and MG in the range of 0.24–31.25 μM. (R 2 ≥ 0.9994). Intra- and inter-day precision yielded RSD values ranging from 0.59 % to 5.78 %, with average recoveries between 99.78 % and 108.50 %. The detection limits were 0.85–12.85 fmol. This method was applied to analyze dried saliva samples from 44 healthy volunteers (HVs) and 46 diabetic patients (DPs), revealing significant differences ( p < 0.001) in the concentrations of 3-DG, GO, and MG between HVs and DPs. The research outcomes validate the practicality of α-DCs in dried saliva samples as prospective biomarker candidates for diabetes, while introducing an innovative methodology for their utilization in clinical settings.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 55
·44571-44577
·
IF 3.84
Shuang-Shuang Zhang,Kun Cui,Jin Huang,Qiao-Ling Zhao,Shao-Kui Cao,Zhi Ma
A thiol–ene “click” reaction proceeds with facile reaction conditions for complete conversion, and displays a higher tolerance to various backbones and functional groups compared with traditional coupling and functionalization strategies. Herein, well-defined α,ω-telechelic polystyrenes with trithiocarbonate and carboxyl terminal groups (PS-CTA) were firstly synthesized via reversible addition-fragmentation chain transfer (RAFT) radical polymerization. Then, the terminal thiol group was converted from the thiocarbonylthio end group of PS-CTA and subsequently reacted with n-butyl acrylate and vinyl ferrocene, respectively, through thiol–ene “click” chemistry, to achieve α,ω-telechelic polystyrenes with difunctionality. Alternatively, a facile one-pot simultaneous aminolysis and thiol–ene “click” reaction using PS-CTA and various ene-bearing compounds as reactants was found to have high efficiency in synthesizing diverse α,ω-telechelic polystyrenes with di- and tri-functionality. Various functional groups such as hydroxyl, acrylate, fluorinated acrylate, ferrocene and allyl, etc. can be successfully incorporated as terminal groups of α,ω-telechelic polystyrenes.
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2021
·
IF 3.205
Qi Dong,Na Hu,Huilan Yue,Honglun Wang,Jinliang Ku
Extracts from the bran of Chenopodium quinoa Willd. (QBE) were reported to be active in inhibiting α-glycosidase, a promising target for treatment of diabetes mellitus. However, the constituents responsible for the α -glucosidase-inhibiting activity of QBE have not been fully characterized. The present study aimed to set up a method for rapid identification of glycosidase inhibiting compounds from the quinoa bran. With surface plasmon resonance (SPR) coupled with liquid chromatography-mass spectrometry (LC-MS), we identified eight flavonoids and ten triterpenoid saponins that may bind to the α-glycosidase. Analysis of the interaction kinetics by molecular docking supported their α -glucosidase-inhibiting activity and revealed the potential mechanisms for the inhibitory effects. In summary, this study established a SPR and LC-MS-based method for rapid in vitro screening of α -glucosidase inhibitors and suggested the quinoa bran a potential natural source of α- glucosidase inhibitors.
✓ 已验证
DOI
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2020
·Vol. 221
·期 6
·1900557
·
IF 2.335
Yiran Wang,Xianhong Zhang,Yuhong Ma,Dong Chen,Changwen Zhao,Wantai Yang
This study reports a step-growth click-polymerization of 1,4-benzenedimethane (BDMT) and diethylene glycol divinyl ether (DEGVE) with 4-( N,N -diphenylamino)-benzaldehyde (DPAB) as a photoredox catalyst under irradiation of visible light. DPAB exhibits a strong UV–vis absorption at 350 nm and a strong fluorescence emission at 480 nm in anisole. There is a strong fluorescence quenching between BDMT and DPAB. The molecular weight of the polythiolether can be controlled by reaction time and monomer feed ratios. More importantly, α,ω-dithiol and α,ω-divinyl telechelic polythiolether oligomers are successfully synthesized by simply changing the molar ratios of BDMT to DEGVE. 1 H NMR and MALDI-TOF MS spectra demonstrate that the oligomers have high end group fidelity. In addition, strong fluorescence is observed when the α,ω-dithiol terminated polythiolether adds with N -(1-pyrenyl) maleimide, indicating that the as-prepared polythiolether bears reactive thiol end groups. Furthermore, high molecular weight polythiolether are prepared by chain extension with reactive polythiolether oligomers as macro-monomers. For example, α,ω-divinyl oligomer ( M n = 2000 g mol −1 ) could further react with α,ω-dithiol oligomer ( M n = 2400 g mol −1 ) to form high molecular weight polythiolether ( M n = 6000 g mol −1 ).
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Hao Zhang,Shu Wang,Shixiao Wei,Shangde Sun,Yanlan Bi,Mingfu Wang,Yueliang Zhao
A novel supercritical fluid chromatography coupled with tandem mass spectrometry (SFC-ESI-QqQ-MS/MS) method was developed for simultaneous quantification of malondialdehyde (MDA) and seven α,β-unsaturated aldehydes (α,β-UAs) in edible oils and oil-containing foods. Combining DNPH derivatization with one-step extraction, the method achieved exceptional performance: linearity (R 2 ≥ 0.9995), rapid analysis (16 min/sample), recoveries (85.84–106.71 %), and precision (RSD < 7.86 %), with LOD/LOQ at 0.05–0.60/0.15–1.80 μg/kg. This validated technique was subsequently applied to monitor the formation of MDA and α,β-UAs during the heating of eight edible oils, revealing concentration dependencies on fatty acid composition and heating duration. Furthermore, the levels of MDA and seven α,β-UAs in 29 oil-containing foods commonly consumed in China were determined using the developed method, with concentrations ranging from 2.65 to 326.79 μg/kg and 15.67–770.71 μg/kg, respectively. As the first SFC-based approach for comprehensive aldehyde profiling, this method enables precise monitoring of lipid oxidation pathways and supports quality control innovations in the food industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Hui-Wen Wang,Jia-Qi Wang,Yue-Qiu,Tong-Hao Cui,Jian-Cong Liao,Yi-Long Liu,Ya-Qin Liu,Yuan-Jiang Pan,Xian Li,Xiao-Yong Zhao
Universal 5-Da labeling creates internal standards for accurate N-glycome analysis. • Novel matrix 2-AIA/CHCA ensures uniform co-crystallization and high reproducibility. • H3N2F1X1 is a cross-species candidate biomarker for fruit cold stress. N-glycosylation is a crucial post-translational modification regulating protein function in plants. However, its accurate quantification by matrix-assisted laser desorption/ionization-mass spectrometry with time-of-flight is challenged by low glycoprotein abundance, poor glycan ionization, uneven matrix crystallization, and insufficient internal standards. Here, we introduce a novel 5-Da stable isotope labeling method, combining enzymatic 18 O-labeling (+2-Da) and reduction with NaBD 4 (+3-Da) on a portion of the sample itself to universally generate internal standards that eliminate isobaric interference. Furthermore, a binary matrix of 2-aminoisophthalic acid (2-AIA) plus α-cyano-4-hydroxycinnamic acid (CHCA) was developed, providing high sensitivity and homogeneous co-crystals. The integration of isotope labeling with 2-AIA/CHCA allows linear quantification ( R 2 > 0.99) over a two orders of magnitude. Applied to tomato and peach during cold storage, the method revealed that H3N2F1X1, the most abundant N-glycan in both fruits, was significantly downregulated, suggesting its role as a candidate biomarker for cold stress response and fruit quality control. Download: Download high-res image (204KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
Materials Today Bio
·
2026
·
IF 11
Hui Zhang,Xu Zhu,Jingchun Wang,Yibo Huo,Yikai Ma,Shengzhong Rong,Yuting Lu,Yingxue Jin
To address the core issue of low clinical response rates (10%-30%) to α-PD-L1 immune checkpoint blockade therapy, this study targeted the immunosuppressive bottleneck mediated by lactate accumulation in the tumor microenvironment (TME). A targeted drug delivery system (HLC) was constructed using hyaluronic acid (HA) nanomicelles co-loaded with lactate oxidase (LOX) and copper-doped carbon dots (CuCDs). Combined with α-PD-L1, a synergistic antitumor strategy integrating lactate metabolic regulation, cuproptosis, and immune activation was established. HLC achieves targeted accumulation through specific binding between HA and CD44 receptors on tumor cells. It directionally releases LOX and CuCDs in the acidic TME via pH-responsive properties. LOX efficiently clears lactate and elevates pH, blocking the HIF-1α/PD-L1 pathway to reverse immunosuppression. It also generates H 2 O 2 in situ to provide substrates for Cu-mediated Fenton-like reactions, enhancing cuproptosis. In vitro experiments confirm that HLC promotes dendritic cell maturation and drives M2-type macrophage polarization toward the M1 phenotype. In vivo studies using CT26 tumor-bearing mouse models show that HLC combined with 808 nm laser and α-PD-L1 significantly inhibits primary and distant tumor growth. It remodels the antitumor immune microenvironment with favorable biosafety. This study elucidates the synergistic mechanism between lactate metabolic regulation and cuproptosis, establishes an association between lactate level and cuproptosis sensitivity, and provides a novel strategy and experimental basis for combined cancer immunotherapy with important clinical translation value.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale Research Letters
·
2019
·Vol. 14
·期 1
·1-11
·
IF 3.159
Zhang Jijun,Chen Zexiang,Wang Yan,Yan Xinyu,Zhou Zhiyu,Lv Huifang
High electrochemical performance of asymmetric supercapacitor (ASC) depends on exquisite nanostructure design and synthesis of electrodes, including structural controllable design and synthesis of high theoretical performance materials and nanocomposite materials. Herein, a template-free hierarchical core-shell nanostructure of CoNiO 2 @NiAl-layered double hydroxide (NiAl-LDH) and α-Fe 2 O 3 with a hollow spherical structure composed of nanoparticles are successfully prepared. The CoNiO 2 @NiAl-LDH as the cathode electrode and the hollow spherical α-Fe 2 O 3 as the anode electrode of the ASC devices exhibit superior electrochemical performance. The gel of polyvinyl alcohol (PVA) and KOH acts as the solid electrolyte and the separator to assemble into the all-solid-state flexible ASC devices. Two of the CoNiO 2 @NiAl-LDH//α-Fe 2 O 3 ASC devices in series are fabricated to meet the voltage requirement of mobile energy equipment, which exhibit a maximum energy density of 65.68 Wh kg −1 at the power density of 369.45 W kg −1 . Interestingly, in addition to many advantages that the solid electrolyte in ASC devices already have, we find that it can be an alternative way of solving the problem of iron oxide cycling performance, and of course it can also be used as a reference for other materials with poor cycling performance.
✓ 已验证
DOI
PubMed
高影响因子
BRAIN RESEARCH BULLETIN
·
2018
·
IF 3.44
Tianhang Wang,Jiling Cheng,Shuyu Wang,Xudong Wang,Hongquan Jiang,Yueqing Yang,Ying Wang,Chunting Zhang,Weiwei Liang,Honglin Feng
Amyotrophic lateral sclerosis (ALS) is a degenerative disease with a progressive loss of motor neurons in the central nervous system (CNS). However, there are unsolved problems with the therapies for this disease. α-Lipoic acid (LA) is a natural, universal antioxidant capable of scavenging hydroxyl radicals as well as regenerating a series of antioxidant enzymes that has been widely used in clinical settings. This study aimed to evaluate the antioxidant and neuroprotective effects of LA in ALS cell and Drosophila models with mutant G85R and G93A hSOD1 genes. The biological effects of LA and the protein levels of several antioxidant factors were examined, as were those of phospho-Akt and phospho-ERK. Furthermore, specific inhibitors of the PI3K/Akt and MEK/ERK signaling pathways were used to analyze their effects on LA-induced antioxidant expression in vivo and in vitro . Evidences showed that the mutant hSOD1 resulted in the increased oxidative stress, abnormal antioxidant signaling and pathological behaviors in motor performance and survival compared with non-mutant hSOD1 models, treatment with LA improved motor activity and survival in transgenic flies, prevented NSC34 cells from mutant hSOD1 or H 2 O 2 induced decreased antioxidant enzymes as well as increased ROS levels. In addition, LA regulated the expression levels of antioxidant proteins in a dose- and periodical time-dependent manner, which might be mediated by ERK/Akt pathway activation and independent from the mutant hSOD1 gene. Our observations suggest that LA exerts strong and positive antioxidant and neuroprotective effects through the activation of the ERK-Akt pathway in hSOD1 ALS models.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）