APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Yu Wu,Lingchi Zeng,Yancheng Zheng,Fuchang You,Xuejin Liu
In this study, nitrogen-doped carbon dots (N-CDs) were synthesized via a one-step hydrothermal method using pectin polysaccharide as the carbon source, with 1-hexylamine, 1,2-diaminocyclohexane, and diethylenetriamine as individual nitrogen sources. The chemical composition, fluorescence properties, and morphology of the synthesized N-CDs were characterized. Their corrosion inhibition performance on N80 steel in 1 M H 2 SO 4 was evaluated using electrochemical analyses and weight loss experiments. Tafel and EIS analyses demonstrated that at a concentration of 200 mg/L, the maximum inhibition efficiencies of PHA-CDs, PCA-CDs, and PDA-CDs, synthesized using monoamine, diamine, and triamine as nitrogen sources, were 75.3 %, 90.1 %, and 95.4 %, respectively. The inhibition efficiency increased with the total nitrogen and pyrrolic N content in the N-CDs, corresponding to the choice of nitrogen source. Weight loss experiments further confirmed these trends. Additionally, the N-CDs were found to act as mixed-type inhibitors with a predominant anodic inhibition effect and are classified as predominantly chemisorptive. The adsorption behavior adhered to the Langmuir adsorption model, where coordination complexes formed between pyrrolic N and metal Fe increase the surface coverage of the adsorption film, thereby enhancing corrosion resistance.
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JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
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2024
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IF 5.8
Yingying Zhang,Yue Zhang,Bo Li,Xinyan Jin,Tong Liu,Ke Zhang,Lili Fu,Wei Qi,Qi Zhang,Bin Li
Two Heated Tobacco Products (HTPs) with different airflow pathways were tested in this study to give insight into the aerosol formation and transfer. One permit the puffing air to flow through the tobacco rod and known as the open-ended system, while the other one sealed the tobacco rod and known as the closed-ended system. Results showed that in both systems, with the heating temperature rising, the aerosol transfer rates of nicotine, 1,2-propylene glycol (PG), and glycerol (VG) increased linearly, meanwhile the residual rates in the tobacco section decreased exponentially. In particular, lower residual rates of nicotine and PG were observed in the closed-ended system compared to open-ended system from 200 to 300 ℃, indicating a higher releasing efficiency in close-ended system. However, higher aerosol transfer rates of nicotine in the closed-ended system were noted merely among 200–260 ℃. Additionally, the transfer rates for PG remained basically the same in both systems. For nicotine, the closed-ended system exhibited lower retention rates between 200 and 240 ℃, but reversed between 260 and 300 ℃. Furthermore, the closed-ended system showed significantly higher retention rates for PG compared to the open-ended system from 200 to 300 ℃. Regarding puff-by-puff aerosol release pattern, both systems showed similar trend of increase-decrease for the same key component at identical temperature. While the single-puff release amount differed between them, for example, 3.8 mg for close-ended and 3.0 mg for open-ended of water at the second puff. Furthermore, apparent release kinetics parameters were obtained based on the first-order reaction kinetic model and the Arrhenius Equation. In the closed-ended system, lower activation energy of nicotine (26.255 KJ·mol −1 compared to 34.896 KJ·mol −1 in open-ended) resulted in lower activation energy barrier, and therefore a more efficient release and transfer rate for nicotine.
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JOURNAL OF MOLECULAR RECOGNITION
·
2024
·Vol. 37
·期 5
·e3100
·
IF 2.3
Jiachen Li,Xiaoting Dong,Yeli Zhang
Metallo-β-lactamases (MβLs) hydrolyze and inactivate β-lactam antibiotics, are a pivotal mechanism conferring resistance against bacterial infections. SMB-1, a novel B3 subclass of MβLs from Serratia marcescens could deactivate almost all β-lactam antibiotics including ampicillin (AMP), which has posed a serious threat to public health. To illuminate the mechanism of recognition and interaction between SMB-1 and AMP, various fluorescence spectroscopy techniques and molecular dynamics simulation were employed. The results of quenching spectroscopy unraveled that AMP could make SMB-1 fluorescence quenching that mechanism was the static quenching; the synchronous and three-dimensional fluorescence spectra validated that the microenvironment and conformation of SMB-1 were altered after interaction with AMP. The molecular dynamics results demonstrated that the whole AMP enters the binding pocket of SMB-1, even though with a relatively bulky R1 side chain. Loop1 and loop2 in SMB-1 undergo significant fluctuations, and α2 (71–73) and local α5 (186–188) were turned into random coils, promoting zinc ion exposure consistent with circular dichroism spectroscopy results. The binding between them was driven by a combination of enthalpy and entropy changes, which was dominated by electrostatic force in agreement with the fluorescence observations. The present study brings structural insights and solid foundations for the design of new substrates for β-lactamases and the development of effective antibiotics that are resistant to superbugs.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Min Zhu,Wen Liu,Shan Su,Meng Gong,Guangneng Liao,Fudong Fu,Gen Chen,Zhiyong Rao,Jingqiu Cheng,Jingping Liu,Yanrong Lu,Younan Chen
Diabetic cardiomyopathy (DC) refers to the abnormal myocardial structure and performance induced by diabetes. Although numerous studies have been carried out, the pathophysiological mechanisms of cardiovascular disorders during diabetes have not been fully clarified. Here, we compared the cardiomyopathy of healthy rhesus monkeys and rhesus monkeys with a history of streptozocin induced type 1 diabetes (T1D) over 7 years. Through comparing the cardiac function using echocardiography, and detecting the serum biochemical indexes, and changes of left ventricle (LV), we found that decreased systolic function, higher blood glycosylated hemoglobin A1c (HbA1 C ) level, hyperglycemia, and hyperlipidemia were early events in diabetic rhesus monkeys. In addition, cardiac histological analysis showed mildly fibrosis and early myocardial hypertrophy, as evidenced by increased Sirius red stained area and cross-sectional area of left ventricle. Transcriptome results revealed that the nutrients metabolism and extracellular matrix related pathways were markedly changed in the left ventricle of diabetic monkeys. Targeted metabolomics and targeted lipid metabolomics further revealed that disturbed amino acid metabolism and lipid accumulation in the LV of diabetic monkeys manifested by accumulated branched chain amino acids (BCAAs) and triglycerides (TAGs), and reduced contents of sphingolipids, glycerophospholipids, cholesteryl esters and carnitines. In conclusion, we reported here for the first time that diabetes lasting for more than 7 years leads to some early pathological changes of myocardium in rhesus monkeys. The cardiac function is mildly compromised and the reprogramming of lipids and amino acids metabolism might play important roles in the progression of DC.
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ACS Nano
·
2025
·
IF 16
Jinzhao Liu,Meicen Wu,Chang Yang,Yang Zhou,Xinran Qi,Kang Chen,Xiaoping Zhang,Ni Fan,Changyou Zhan,Weiping Wang
Metastasis and immune escape play crucial roles in the progression of colon cancer. Current chemotherapy and immunotherapy treatments have limited effectiveness in reversing the immunologically cold microenvironment. Therefore, it is essential to investigate efficient strategies to enhance the antitumor immunity. In this study, ferroptosis suppressor protein 1 (FSP1) siRNA and photosensitizer Verteporfin were incorporated into the lipid nanoparticle (LNP) formulation for synergistic colon cancer immunotherapy. We first screened the photosensitive LNP formulations using different types of light-responsive small molecules. The selected photosensitive LNPs were further optimized by modulating surface properties to improve cellular uptake and endosomal escape levels in colon cancer cells. Upon light irradiation, the engineered photosensitive LNPs promoted synergistic ferroptosis by FSP1 gene silencing and oxidative stress, thereby causing immunogenic cell death to stimulate dendritic cell maturation and cytotoxic T lymphocyte response. In a bilateral tumor model, photosensitive LNPs exhibited potent antitumor efficacy and robust immunostimulatory effect upon light irradiation, enabling safe and efficient colon cancer treatment. This study demonstrates a synergistic potential between FSP1 gene silencing and photodynamic therapy, expanding the applications of photosensitive LNPs in clinical cancer treatment.
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ACS Sensors
·
2025
·
IF 9.1
Lingxuan Qu,Lingyi Meng,Xilin Sun,Wei Cui,Jin Shi,Yuqi Wang,Qingshuang Hu,Shuping Xu,Bingxin Sun,Chongyang Liang
Aiming at realizing field detection during HIV-1 patient screening in developing countries and point of care testing (POCT) of virus levels in HIV-1 patients receiving medication, an urgent demand for portable nucleic acid detection technology with low cost and sensitivity is raised. To solve this, a CRISPR/Cas13a-based electrochemical detection platform by a multiple combined crRNAs strategy is developed. This sensing platform is based on the Ion Current Rectification regulation through a Porous Anodic Alumina membrane decorated with ssRNA chains, which are trans-cleavage substrates for activated Cas13a and become shorter when the target gene exists, resulting in an altered motion of transmembrane ions. This platform was proven to be feasible and ultrasensitive for tracing the HIV-1 virus by multiple applicable crRNAs combination, and its clinical sample detection limit arrives at 60 copies/μL for HIV-1 patients’ serum samples. Importantly, this method is amplification-free, and its sensitivity is beyond the nonamplification fluorescence-based nucleic acid strategy by one order of magnitude. This sensor is facile to use and cost-effective, and can also be extended to other RNA-based pathogenic microorganism detections. This POCT method facilitates self-monitoring of drug resistance in HIV-1 patients, regardless of medication status.
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Materials
·
2025
·Vol. 18
·期 16
·3780
·
IF 3.2
Lei Yang,Anbang Duan,Zhanyin Liu,Tingying Wei,Chunzhao Liu
Aiming to address the key challenges of poor enzyme stability, difficult recovery, and difficult synergistic optimization of catalytic efficiency in high-value conversion of biomass, this study utilizes mineralization self-assembly technology to combine laccase with Fe3O4@SiO2-PMIDA-Cu2+composite, constructing magnetic laccase nanoflower (MLac-NFs) materials with a porous structure and superparamagnetism. This synthetic material can efficiently catalyze the selective oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA). The characterization results indicated that MLac-NFs exhibit optimal catalytic activity (63.4 U mg−1) under conditions of pH 6.0 and 40 °C, with significantly enhanced storage stability (retaining 94.26% of activity after 30 days of storage at 4 °C). Apparent kinetic analysis reveals that the substrate affinity and maximum reaction rate of MLac-NFs were increased by 38.3% and 439.6%, respectively. In the laccase–mediator system (LMS), MLac-NFs mediated by 30 mM TEMPO could achieve complete conversion of HMF to FDCA within 24 h. Moreover, due to the introduction of magnetic nanoparticles, the MLac-NFs could be recovered and reused via an external magnetic field, maintaining 53.26% of the initial FDCA yield after six cycles.
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BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Chengyu Yang,Jie Wei,Yixuan Li,Yuanhao Peng,Xuan Wu,Xianrui Zhang,Yingxin Ding,Shumi Lu,Chongling Feng,Chao Huang
Aging-induced surface passivation of nano zero-valent iron (nZVI) significantly diminishes its reactivity for Cr(VI) reduction, thereby impeding its practical application in environmental remediation. Herein, we propose a novel system integrating aged nZVI (AnZVI), Shewanella oneidensis MR-1, and the endogenous electron shuttle riboflavin (RF) to overcome the passivation limitations and enhance Cr(VI) removal. Remarkably, the AnZVI/MR-1/RF system achieved complete removal of 20 mg L − 1 Cr(VI) within 3 h, outperforming AnZVI/MR-1 and AnZVI alone by 23.8 % and 36.6 %, respectively. Mechanistic investigations revealed that RF mediated direct interfacial contact between AnZVI and MR-1, promoting microbial reduction of the AnZVI passivation layer. Electrochemical analyses confirmed RF’s critical role as an electron mediator in accelerating charge transfer between AnZVI and MR-1. Three-dimensional fluorescence mapping (3D-EEM) analysis revealed microbial extracellular polymeric substances (EPS) contributions to chromium immobilization processes. The immobilization of chromium is primarily attributed to the reduction of Cr(VI) to insoluble Cr(III) species, in conjunction with adsorption and surface complexation mechanisms. TEM validated a significant reduction in the thickness of the iron passivation layer, resulting in the exposure of the underlying Fe⁰ core and a resultant enhancement in reductive capacity. This work provides a robust strategy to overcome AnZVI’s limitations by coupling microbial activity with electron shuttling, offering new insights into sustainable remediation via biotic-abiotic synergies.
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ACS Omega
·
2025
·
IF 4.3
Suyun Rao,Ke Xiao,Xuhui Chen,Xufang Sun
Diabetic retinopathy (DR) is a major cause of vision impairment, with diabetic retinal neurodegeneration (DRN) identified as an early pathological feature. Although dopamine (DA) has demonstrated protective effects in various neurodegenerative diseases, its role in DRN is inadequately understood. This study provides evidence that DA can alleviate DRN impairment and elucidates the underlying mechanisms. Diabetes was induced in C57BL/6 mice through a single large dose injection of streptozotocin, and the dopamine precursor l-DOPA was administered via intraperitoneal injection daily for 2 weeks to therapeutically supplement dopamine levels. The results indicated that l-DOPA treatment significantly restored retinal thickness, enhanced the amplitude of oscillatory potentials, and alleviated retinal glial cell activation compared to diabetic mice. In vitro, DA treatment of SH-SY5Y cells under high-glucose stress led to a significant increase in superoxide dismutase activity, with a reduction in malondialdehyde levels, lipid peroxide, and iron ion concentration. Additionally, the expression of GPX4, SLC7A11, ferritin, Nrf2, and HO-1 proteins was upregulated, while the expression of NCOA4 was downregulated. Importantly, the protective effects of DA were significantly attenuated by the Nrf2 inhibitor ML385, confirming the involvement of the Nrf2/HO-1 pathway in DA’s protective mechanism. These findings suggest that DA alleviates oxidative stress, reduces ferroptosis, and improves the retinal function in DRN through the activation of the Nrf2/HO-1 pathway, indicating that DA may represent a novel therapeutic strategy for treating DRN.
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ENZYME AND MICROBIAL TECHNOLOGY
·
2024
·
IF 3.4
Quan Luo,Guan Zhou,Zhongxia Li,Jiangpeng Dong,Hang Zhao,Huifang Xu,Xuefeng Lu
The chiral amine ( R )-2-(1-aminoethyl)-4-fluorophenol has attracted increasing attentions in recent years in the field of pharmaceuticals because of its important use as a building block in the synthesis of novel anti-tumor drugs targeting tropomyosin receptor kinases. In the present study, a ω-transaminase (ωTA) library consisting of 21 ( R )-enantioselective enzymes was constructed and screened for the asymmetric biosynthesis of ( R )-2-(1-aminoethyl)-4-fluorophenol from its prochiral ketone. Using ( R )-α-methylbenzylamine, D-alanine, or isopropylamine as amino donor, 18 ωTAs were identified with target activity and the enzyme AbTA, which was originally identified from Arthrobacter sp. KNK168, was found to be a potent candidate. The E. coli whole cells expressing AbTA could be used as catalysts. The optimal temperature and pH for the activity were 35–40 °C and pH8.0, respectively. Simple alcohols (such as ethanol, isopropanol, and methanol) and dimethyl sulfoxide were shown to be good cosolvents. High activities were detected when using ethanol and dimethyl sulfoxide at the concentrations of 5–20%. In the scaled-up reaction of 1-liter containing 13 mM ketone substrate, about 50% conversion was achieved in 24 h. 6.4 mM ( R )-2-(1-aminoethyl)-4-fluorophenol was generated. After a simple and efficient process of product isolation and purification (with 98.8% recovery), 0.986 g yellowish powder of the product ( R )-2-(1-aminoethyl)-4-fluorophenol with high ( R )-enantiopurity (up to 100% enantiomeric excess) was obtained. This study established an overall process for the biosynthesis of the high value pharmaceutical chiral amine ( R )-2-(1-aminoethyl)-4-fluorophenol by ωTA. Its applicable potential was exemplified by gram-scale production.
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Marine Drugs
·
2025
·Vol. 23
·期 8
·294
·
IF 5.4
Jing Lu,Pujing Shi,Yutian Cao,Bingxin Shi,Huilin Shen,Shuai Zhao,Yuchen Gao,Huibing Chi,Lei Wang,Yawei Shi
Mussels are nutrient-rich but perishable, resulting in substantial resource loss. A protease-producing strain (Bacillus velezensisZ-1,Mytilus edulis) isolated from marine sludge was used to hydrolyze mussels, producing Y-1, a hydrolysate with antioxidant activity. In this study, ultrafiltration, gel chromatography, and LC-MS/MS were employed to isolate and identify bioactive peptides from the hydrolysate. The results revealed that the hydrolysate exhibited antioxidant activity, pancreatic cholesterol esterase inhibitory activity, pancreatic lipase inhibitory activity, and α-glucosidase inhibitory activity. Molecular docking using AutoDock Tools 1.5.6 was performed to analyze the interactions of peptides with CD38 and Keap1, leading to the identification of five potentially bioactive peptides: VPPFY, IMLFP, LPFLF, FLPF, and FPRIM. These peptides formed hydrogen bonds and hydrophobic interactions with CD38 and Keap1, demonstrating strong DPPH radical scavenging and superoxide anion radical scavenging capacities. This study highlights the multifunctional bioactive potential of these peptides, offering insights into their therapeutic applications. The findings provide a novel approach for the effective utilization of mussel resources and highlight their potential application value in the development of functional foods.
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ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Pengfei Xu,Kangpeng Jin,Yuan Yu,Ming Huang,Zhenhua Yan,Xiang Li,Xiao Gao,Liangwei Fu,Biao Xu
The untapped potential of sub-nanometric materials (SNMs) in constructing high-performance thermoelectrics (TE) presents a promising yet unexplored avenue. This study investigates the utilization of supertetrahedral CdS clusters, representing SNMs, in conjunction with state-of-the-art Bi 2 Te 3 -based systems. The CdS cluster with size-dependent electronic structure enables selective interface scattering of carriers, while its 1 nm size and size-dependent phonon density of states (DOS) contribute to significant interfacial thermal resistance. Furthermore, interfacial dislocations and significant grain refinement induced by the incorporation of the CdS cluster boost the mechanical properties. Consequently, a remarkable peak figure of merit ( ZT ) value of 1.47 at 350 K and a record-high Vickers hardness of 1.08 GPa is achieved in the Bi 0.5 Sb 1.5 Te 3 /0.1 wt% CdS cluster composite. The robust TE module achieves an excellent conversion efficiency of about 6.1% under a ΔT of 241 K. This work opens up exciting possibilities for utilizing 1 nm clusters in thermoelectrics and even inspires other fields involving size-dependent electrical and thermal transport behaviors.
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