npj Science of Food
·
2025
·Vol. 9
·期 1
·1-9
·
IF 7.8
Ye Xiang,Chen Wen,Yan Fujie,Zheng Xiaodong,Tu Pengcheng
In late-stage type 2 diabetes mellitus (T2DM), impaired islet β cell function leads to absolute insulin deficiency, thereby disrupting blood glucose homeostasis. GLP-1, an incretin hormone, stimulates insulin secretion from islet β cells post-meals. This study investigated the effects of anthocyanin cyanidin-3-O-glucoside (C3G) on GLP-1 secretion using STC-1 (intestinal endocrine L cells) and NIT-1 (islet β cells). In a co-culture system, C3G treatment increased GLP-1 secretion in STC-1 cells, promoting insulin release in NIT-1 cells under high glucose. Mechanistically, C3G activated the PPARβ/δ-β-catenin-TCF-4 pathway in STC-1 cells, enhancing PG precursor transcription and GLP-1 synthesis.Inhibiting PPARβ/δ with GSK0660 blocked this C3G-induced upregulation. Overall, C3G stimulates GLP-1 secretion from intestinal L cells via this pathway, indirectly boosting insulin release from β cells. These findings enhance T2DM mechanism understanding and suggest the potential of C3G in GLP-1-based T2DM therapy.
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高影响因子
CHINESE CHEMICAL LETTERS
·
2014
·
IF 1.178
Cheng-Xi Zhang,Yu-Yuan Deng,Yi-Yang Zhang,Po Yang,Yi Gu
To study the synthesis of 3,4-dihydro-2 H -3-phenyl-1,3-benzoxazine (benzoxazine), the reaction paths of phenol, aniline and formaldehyde were investigated by analyzing the synthesis crude products. With the aid of high-performance liquid chromatography (HPLC), chromatographic column and preparative HPLC, seven compounds originated from the crude products were obtained and their chemical structures were elucidated. Possible reaction paths are proposed based on these compounds. Results show that N -hydroxymethyl aniline (HMA) derived from the reaction of formaldehyde and aniline is probably the key intermediate during the reaction. HMA can react with itself or other reactants to form other intermediates, such as 1,3,5-triphenyl-1,3,5-triazinane and 2-((phenylamino)methyl)phenol, and further form benzoxazine and byproducts.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Huanhuan Zhang,Xuan Cai,Hongli Zhao,Wenqian Sun,Zhenxing Wang,Minbo Lan
Developing nanomaterials as alternatives of enzymes is of great significance to realize qualification and quantitation of superoxide anion (O 2 − ), which has been proved to play a crucial role in physiological condition. In this work, a novel electrochemical catalyst of cobalt nanoparticle doped porous cubic carbon (Co-PCC) was prepared through the calcination of zeolitic imidazolate framework-67 (ZIF-67), which was obtained from one-step synthesis. Then Co-PCC was used to construct enzyme-free electrochemical sensor for the detection of O 2 − . The response of H 2 O 2 , though much lower than that of O 2 − , was also caught by the Co-PCC/SPCE. The fabricated sensor exhibited obvious response towards O 2 − in the range of 0.8–76.8 μM (R 2 = 0.997) with a high sensitivity of 3.59 × 10 −4 mA μM −1 cm −2 and a limit of detection of 0.41 μM (S/N = 3). In addition, the proposed sensor can be used to detect O 2 − released from SK-BR-3 cells, which makes it possible to monitor the dynamic change of O 2 − in living cells.
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DALTON TRANSACTIONS
·
2019
·Vol. 48
·期 28
·10537-10546
·
IF 4.052
Guofeng Liu,Yeqing Chen,Mochen Jia,Zhen Sun,Binbin Ding,Shuai Shao,Fan Jiang,Zuoling Fu,Ping'an Ma,Jun Lin
Rare earth ion-doped fluoride upconversion nanoparticles (UCNPs), emerging as a novel class of probes and drug carriers, exhibit superior promise for bio-applications in diagnostics and treatment on account of their strong luminescence, fine biocompatibility, and high drug loading. However, the fine control and manipulation of particle size and the distribution of rare earth ion-doped oxides has remained an insurmountable challenge to date. In this work, we construct and synthesize silica-coated Gd2(WO4)3:Yb3+/Ho3+ nanoparticles by one-pot co-precipitation, with uniform distribution (∼130 nm) and enhanced yellow fluorescence. Particularly, the nanoparticles not only possess outstanding temperature sensing performance at biological temperatures in water by utilizing the fluorescence intensity ratio (FIR) method, but also allow a further serviceable contrast effect in vitro and in vivo based on the prominent T1-weighted magnetic resonance (MR) signal of Gd3+. Compared with cisplatin and platinum(IV) (DSP), the Gd2(WO4)3@SiO2 nanoparticles functionalized with DSP (Gd2(WO4)3@SiO2-Pt-PEG) exert higher lethality against CT26 cells and significantly inhibit the growth of tumors at the same concentration of Pt. This effect occurs through the greater level of cell endocytosis. The lethality value of the latter is 10 times higher than the former after the same length of time according to inductively coupled plasma-mass spectrometry (ICP-MS) results. In short, the monodisperse and strongly fluorescent Gd2(WO4)3@SiO2-Pt-PEG nanoparticles are endowed with dual-mode imaging, temperature sensing and anticancer functions, which provide a significant guide for synthesis and bio-application of lanthanide ion-doped oxides.
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CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Dongmei Wu,Jiaqi Zheng,Weiwei Hu,Xiaoliang Zheng,Qiaojun He,Robert J. Linhardt,Xingqian Ye,Shiguo Chen
Rhamnogalacturonan I (RG-I) pectin are regarded as strong galectin-3 (Gal-3) antagonist because of galactan sidechains. The present study focused on discussing the effects of more structural regions in pectin on the anti-Gal-3 activity. The water-soluble pectin (WSP) recovered from citrus canning processing water was categorized as RG-I pectin. The controlled enzymatic hydrolysis was employed to sequentially remove the α-1,5-arabinan, homogalaturonan and β-1,4-galactan in WSP. The Gal-3-binding affinity KD (kd/ka) of WSP and debranched pectins were calculated to be 0.32 μM, 0.48 μM, 0.56 μM and 1.93 μM. Moreover, based on the more sensitive cell line (MCF-7) model, the IC 30 value of WSP was lower than these of modified pectins, indicating decreased anti-Gal-3 activity. Our results suggested that the total amount of neutral sugar sidechains, the length of arabinan and cooperation between HG and RG-I played important roles in the anti-Gal-3 activity of WSP, not the Gal/Ara ratio or RG-I/HG ratio. These results provided a new insight into structure-activity relationship of citrus segment membrane RG-I as a galectin-3 antagonist and a new functional food.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Lujia Ding,Pengcheng Wang,Qiuhan Lin,Dongxue Li,Yuangang Xu,Ming Lu
Fused cyclic energetic compounds are a unique class of large conjugated structures containing two or more rings, and have been identified as promising competitors for traditional energetic materials. In the present work, we investigated 5-amino-6-oxo-4,6-dihydro-[1,2,5]oxadiazolo[3′,4′:5,6]pyrido[4,3- d ][1,2,3]triazine 8-oxide ( 3 ) in detail as a fused amphoteric energetic molecule that can be protonated and deprotonated by acids and bases to obtain its cationic ( 11 – 13 ) and anionic ( 4 – 10 ) salts. All the new compounds were thoroughly characterized by infrared and multinuclear NMR ( 1 H and 13 C) spectroscopy, and elemental analysis. The structural features of 3 – 6 , 9 , 10 , 12 , and 13 were investigated by single-crystal X-ray diffractions and different theoretical techniques (Hirshfeld surfaces analyses, and noncovalent interactions). The aromaticity of compound 3 was studied via localized orbital locator- π and iso -chemical shielding surfaces (ICSS). Their thermal decomposition temperatures were investigated by differential scanning calorimetry and thermogravimetry to be 310–344 °C (onset). In addition, impact and friction tests revealed that these compounds were insensitive. Densities, heats of formation and detonation properties were measured by a gas pycnometer, calculated using the Born-Haber energy cycle and EXPLO5 v6.05.04 program, respectively. The neutral compound 3 and all its non-metallic salts have better detonation properties than HNS and PYX and lower sensitivities. In particular, salt 11 (N + O: 61.74 %, T d : 330 °C, Δ H f : 1.68 kJ g −1 , D : 8.795 km s −1 , P : 36.0 GPa, IS: > 40 J, FS: > 360 N) is a potential candidate for heat-resistant explosives.
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Acta Biomaterialia
·
2018
·
IF 6.383
Dongni Wu,Yongnu Zhang,Xiaoting Xu,Ting Guo,Deming Xie,Rong Zhu,Shengfeng Chen,Seeram Ramakrishna,Liumin He
In this study, we prepared a multifunctional gene delivery nanovector containing a chitosan (CS) backbone and polyethylenimine (PEI) arms with arginine-glycine-aspartate (RGD)/twin-arginine translocation (TAT) conjugated via polyethylene glycol (PEG). Branched PEI, with a molecular weight of 2000 Da, was used to achieve a balance between biocompatibility and transfection efficiency, whereas RGD/TAT peptides were conjugated for enhanced targeting ability and cellular uptake. Synthesis of the copolymers was confirmed by characterizing the chemical structure with 1 H nuclear magnetic resonance and Fourier Transform Infrared Spectroscopy (FTIR). The nanovector was biocompatible with cells and showed excellent capability for DNA condensation ; the resulting complexes with DNA were well-formed, and possessed small particle size and reasonable positive charge. Higher gene transfection efficiency, compared to that achieved with PEI (25 kDa), was confirmed in tumor (HeLa cells) and normal cells (293T and NIH 3T3 cells). More importantly, the cells transfected with the chitosan- graft -PEI-PEG/pCMV-EGFP-Ntf3 complex produced sustained neurotrophin-3 with a linear increase in cumulative concentration, which induced neuronal differentiation of neural stem cell and promoted neurite outgrowth . These findings suggested that our multifunctional copolymers might be ideal nanovectors for engineering cells via gene transfection, and could potentially be applied in tumor therapy and regenerative medicine. Statement of Significance We successfully prepared a multifunctional gene delivery nanovector containing branched PEI with a molecular weight of 2000 Da to balance between biocompatibility and transfection efficiency, and RGD/TAT peptides for enhanced targeting ability and cellular uptake. The well-formed CPPP/DNA complexes of small particle size and reasonable positive charges potentially enhanced gene transfection in both tumor and normal cells. More importantly, the CPPP/pCMV-EGFP-Ntf3 complex-transfected 293T cells could produce sustained NT-3 with a constant ratio, which induced neuron differentiation of NSC and promoted neurite outgrowth. Therefore, our study provided an effective strategy for producing neurotrophins by engineering cells with gene delivery, which deserved wide investigation and potential application in regenerative medicine.
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Journal of Advanced Dielectrics
·
2022
·
IF 0
Xudong Qi,Kai Li,Lang Bian,Enwei Sun,Limei Zheng,Rui Zhang
Relaxor-based ternary Pb( In 1 / 2 Nb 1 / 2 )O 3 –Pb( Mg 1 / 3 Nb 2 / 3 )O 3 –PbTiO 3 (PIN–PMN–PT) single crystals and ceramics are promising candidates for high-performance electromechanical conversion devices. It is known that the domain structure and dielectric diffusion–relaxation characteristics are crucial to the excellent performances of relaxor ferroelectrics. In this work, we prepared the PIN–PMN–PT ceramics with various PIN/PMN proportions and systematically investigated their domain structure and dielectric diffusion–relaxation properties. The effect of PIN/PMN proportion on the domain size and dielectric diffusion–relaxation characteristics was also studied. The investigations showed that PIN–PMN–PT ceramics presented multi-type domain patterns comprising irregular island domains and regular lamellar domains. Moreover, the dependent relations of PIN/PMN proportions on the dielectric diffusion and domain size indicated that the PIN composition has a stronger lattice distortion than PMN composition; increasing the PIN proportion can enhance the dielectric diffusion and decrease the domain size. Our results could deepen the understanding of structure–property relationships of multicomponent relaxor ferroelectrics and guide the design and exploration of new high-performance ferroelectric materials.
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Food Science & Nutrition
·
2024
·
IF 3.5
Jialing Lin,Chuanhui Zeng,Xueli Li,Qin Tang,Jing Liao,Yan Jiang,Xianchun Zeng
Heterocyclic amines (HAs) are a group of mutagenic and carcinogenic compounds produced from the processing of high-protein foods, which include 2-amino-3-methylimidazo[4, 5-f]quinoline (IQ) showing the strongest carcinogenic effect. Camels are able to digest HAs in foods, which provide rich microbial resources for the study. Thus, camel rumen and intestinal microbiota were used to degrade IQ, and the dominant microorganisms and their degradation characteristics were investigated. After three generations of culture with IQ as the sole carbon source, the highest abundance in rumen and intestinal microbes was found in the Proteobacteria phylum. The strains of third generation of the rumen contents were mainly attributed to the genera Brevundimonas and Pseudomonas , and the dominant genera in intestine were Ochrobactrum , Bacillus , and Pseudomonas . Microorganisms were further isolated and purified from the third generation cultures. These 27 strains from the rumen (L1–L27) and 23 strains from the intestine (C1–C23) were obtained. Among them, four strains with the most effective degrading abilities were as follows: L6 (28.55% of IQ degrading rate) and C1 (25.19%) belonged to the genus Ochrobactrum , L15 (23.41%) belonged to the genus Pseudomonas , and C16 (20.89%) were of the genus Bacillus . This study suggested the application of abundant microbial resources from camels' digestive tract to biodegrade foodborne toxins.
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MACROMOLECULES
·
2020
·
IF 5.918
Dalong Zhai,Mingjing Zhu,Shuwen Chen,Yue Yin,Xin Shang,Lixin Li,Gang Zhou,Juan Peng
Despite recent impressive advances in synthesis of all-conjugated diblock copolymers via facile quasi-living Grignard metathesis (GRIM) polymerization, it remains challenging to achieve well-defined all-conjugated triblock copolymers of interest. Herein, we report the judicious design and synthesis of a series of all-conjugated triblock copoly(3-alkylthiophene)s consisting of poly(3-butylthiophene) (P3BT), poly(3-hexylthiophene) (P3HT), poly(3-octylthiophene) (P3OT), or poly(3-dodecylthiophene) (P3DDT) in all 12 possible combinations. The effects of block sequences and the length of alkyl side chains on their cocrystallization and microphase-separated structures are investigated. Moreover, the correlation between different crystalline structures and charge mobilities in organic field-effect transistors (OFETs) is scrutinized. These rationally synthesized triblock copoly(3-alkylthiophene)s self-assemble into cocrystals with an edge-on orientation in as-cast and 150 °C-annealed samples. Remarkably, the combinations with the shortest alkyl side chains placed as the central block (i.e., P3HT-b-P3BT-b-P3OT, P3HT-b-P3BT-b-P3DDT, P3OT-b-P3BT-b-P3DDT, and P3OT-b-P3HT-b-P3DDT) exhibit higher film crystallinity and charge mobilities than the other two combinations where the blocks with shortest alkyl side chains are located at two terminals. Intriguingly, upon thermal annealing near the melting temperature of these combinations, P3BT-b-P3OT-b-P3HT, P3BT-b-P3DDT-b-P3OT, and P3HT-b-P3DDT-b-P3OT can retain their cocrystalline structure while the other combinations are found to microphase separate. Finally, the all-conjugated triblock copoly(3-alkylthiophene)s with proper block sequence demonstrate stable charge mobilities at high temperatures and good flexibility in OFET devices. Taken together, this study elucidates that the block sequence is of key importance on control of the crystallization and charge transport behavior of poly(3-alkylthiophene)-based triblock copolymers.
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MATERIALS & DESIGN
·
2022
·
IF 9.417
Yanfang Zhou,Mianrong Chen,Piaoyi Chen,Xiaotian Zhao,Hua Zhou,Jingmiao Wu,Xufeng Li,Qiuxia Li,Runxing Lin,Yugang Huang,Yingying Lun,Guodong Ye
Preparing polymers from allyl monomers without being hindered by degradative chain transfer (DCT) remains a challenge. Herein, we report the synthesis and characterization of embolic microspheres based on allyl monomers for transcatheter arterial embolization using a photodriven radical-mediated [3 + 2] cyclization reaction (PRMC) without metal catalysis. The mechanism of PRMC was validated by thermal and spectral experiments, indicating that PRMC overcomes the restrictions of DCT and makes it possible to synthesize a variety of well-defined polymers from inert allyl monomers. The embolization microspheres based on poly(sucrose allyl ether) were characterized by morphological determination, degradation and swelling tests. Cell experiments show that the microspheres have good biocompatibility. The complete arterial occlusion of the embolized kidneys was observed, indicating that microspheres have broad application prospects in embolization interventional therapy.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2020
·
IF 4.65
Z.B. Feng,B.J. Tao,W.F. Wang,H.Y. Liu,H.T. Wu,Z.L. Zhang
Novel low temperature sintered Li 4 Mg 3 [Ti 0.8 (Mg 1/3 Ta 2/3 ) 0.2 ] 2 O 9 (LMT 0.8 (MT) 0.2 ) ceramics with 1–5 wt% LiF additives were successfully prepared by the solid-state reaction method. The effects of LiF additives on the phase composition, sintering characteristic, microstructure and microwave dielectric properties of LMT 0.8 (MT) 0.2 ceramics were investigated in detail for the first time. The sintering temperature of LMT 0.8 (MT) 0.2 ceramics could be effectively lowered to 950 °C by the addition of LiF. A single rock salt crystalline phase structure belonging to a space group of Fm-3m (No.225) was obtained through X-ray diffraction patterns. Dense uniform morphology was observed at 950 °C for LMT 0.8 (MT) 0.2 ceramics with 3–5 wt% LiF in terms of scanning electron microscopy photographs. Particularly, the LMT 0.8 (MT) 0.2 -4 wt% LiF ceramics sintered at 950 °C for 4 h possessed optimum microwave dielectric properties of ε r = 16.10, Q·f = 114,313 GHz (at 8.18 GHz) and τ f = −7.72 ppm/°C. In addition, the excellent chemical compatibility with silver metal electrodes indicated that the LMT 0.8 (MT) 0.2 -4 wt% LiF ceramics might be a promising candidate for the low-loss low temperature co-fired ceramics (LTCC) applications in microwave devices.
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危险品化学品经营许可证（带存储）