Frontiers in Pharmacology
·
2025
·
IF 4.8
Shuzhen Mao,Jiahui Huang,Junyan Li,Fang Sun,Qilian Zhang,Qing Cheng,Wei Zeng,Dongya Lei,Shiyan Wang,Jing Yao
Introduction: Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) characterized by mucosal damage and impaired epithelial barrier function. While glycyl-l-histidyl-l-lysine-copper (GHK-Cu) exhibits known anti-inflammatory properties, its therapeutic mechanisms in UC remain undefined. This study was designed to systematically evaluate the therapeutic potential of GHK-Cu in a dextran sulfate sodium (DSS)-induced murine model of UC, with particular emphasis on elucidating its regulatory effects on the NAD-dependent deacetylase sirtuin-1 (SIRT1)/signal transducer and activator of transcription 3 (STAT3) signaling pathway.Methods: UC was induced in BALB/c mice with 3% DSS for 14 days. The protein expression levels of tight junction associated protein-1 (ZO-1), Occludin, inflammatory factors interleukin (IL)-6, IL-1β and tumor necrosis factor (TNF)-α, SIRT1, STAT3, p-STAT3, and retinoic acid receptor-related orphan receptor gamma t (RORγt) were detected by Western blot. Histopathological changes were evaluated by Hematoxylin and Eosin (H&E) and Alcian blue-periodic acid-Schiff (AB-PAS). Network pharmacology and molecular docking were used to predict the core targets of GHK Cu in the treatment of UC. An in vitro UC model was also established in mouse peritoneal macrophages (MPMs) using lipopolysaccharide (LPS), and a co culture model was constructed using mouse colonic epithelial cells (MCECs) and MPMs to examine the role of GHK Cu in promoting mucosal healing. STAT3 was silenced by gene transfection technology to verify the core role of STAT3 in GHK Cu treatment of UC.Results: GHK-Cu alleviated weight loss, improved the disease activity index (DAI), reduced colonic edema and shortening, attenuated inflammatory damage, increased goblet cell numbers, suppressed inflammatory cytokines such as TNF-α, IL-6, and IL-1β, and promoted mucosal repair. Additionally, a co-culture system of MCECs and MPMs revealed that GHK-Cu facilitated MCECs healing, impaired by DSS, by upregulating ZO-1 and Occludin expression. Subsequently, network pharmacology and molecular docking identified SIRT1 as a potential target of GHK-Cu. Results showed that GHK-Cu upregulated SIRT1 protein expression and suppressed the expression of phosphorylated p-STAT3 in colon tissue and MCECs of the co-culture model. Our findings revealed that after transfection with STAT3-targeting siRNA (siSTAT3), the stimulant effect of GHK-Cu on the healing of MCECs and the effect on the protein expression of ZO-1 and Occludin is canceled. Nevertheless, after transfection with siSTAT3, it could inhibit the expression of inflammatory factors in conjunction with GHK-Cu. Furthermore, we found that GHK-Cu could inhibit RORγt expression in the colon tissue of UC mice.Discussion: This study found that GHK-Cu demonstrated significant therapeutic effects in DSS-induced UC in mice. GHK-Cu may promote mucosal healing and enhance tight junction protein expression by regulating the SIRT1/STAT3 pathway. In addition to suppressing p-STAT3 expression, GHK-Cu may utilize additional pathways to inhibit inflammatory factors. Furthermore, GHK-Cu may reduce the number of Th17 cells. In summary, GHK-Cu may treat UC by acting on the SIRT1/STAT3 pathway.
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ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Ziyang Xu,Ying Shen,Liang Chen,Chunbo Wang
Direct CO2 hydrogenation from primary carbon sources such as flue gas offers an attractive route for carbon-neutral fuel synthesis, yet the presence of O2 severely impairs catalyst performance due to preferential H2–O2 reactions and subsequent catalyst deactivation. Herein, we report a surface-engineered Ni/γ-Al2O3 (Ni/A) catalyst modified by Cu doping (Ni–Cu1/A), which exhibits enhanced oxygen resistance and sustained CH4 produce under oxidative conditions. Guided by a descriptor τ, defined as the ratio of O2 to CO2 adsorption energies, Cu was identified as an effective dopant to suppress O2 adsorption while preserving CO2 activation. Under a challenging atmosphere containing 6% O2, the optimized Ni–Cu1/A catalyst achieved a CH4 STY of 76.4 mmol gcat–1 h–1, significantly outperforming the undoped Ni/A catalyst, which drops to 63.1 mmol gcat–1 h–1 under the same conditions. Mechanistic analysis reveals that Cu incorporation weakens O2 adsorption by regulation of electronic structure, thereby mitigating exothermic H2–O2 side reactions and stabilizing Ni0 sites. This work presents a practical and scalable strategy for CO2 methanation in the presence of O2, offering a promising approach for direct catalytic conversion of flue gas by leveraging selective surface adsorption and electronic structure modulation.
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INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
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IF 5.4
Yisha Zheng,Huiqin Zhang,Qiuwen Huang,Yanhui Zhang,Xian Zhang,Wenlong Xiang
Tetracycline hydrochloride (TCH), as a representative antibiotic pollutant, poses significant environmental risks due to its persistence and ecotoxicity. Conventional degradation technologies are often hampered by inefficiency and high operational costs. To overcome these limitations, a simple sodium borohydride (NaBH 4 ) reduction method was employed to modify CoFe Prussian blue analogue (CoFe-PBA), significantly its intrinsic activity. Subsequently, low-level Cu loading (2 wt%) was introduced, further substantially boosting the degradation efficiency. Comprehensive characterization confirmed that this sequential modification optimizes the material structure and enhances the density of active sites. The optimized 2 %Cu/CoFe PBA catalyst efficiently activated peroxymonosulfate (PMS), achieving >96 % TCH removal within 30 min under optimal conditions. In addition, the 2 %Cu/CoFe PBA exhibited excellent stability across a broad pH range (5–11) and in complex anionic environments. Radical quenching experiments and mechanism analysis revealed that non-radical singlet oxygen ( 1 O 2 ) and sulfate radical (SO 4 · − ) pathways were the dominant contributors to TCH degradation, with superoxide radical (O 2 · − ) and hydroxyl radical (∙OH) playing comparatively minor roles. This high performance stems from synergistic Fe/Co/Cu interactions promoting efficient PMS activation. This work provides a facile, efficient modification strategy (NaBH 4 reduction and minimal Cu loading) for PBAs and clarifies the predominant reactive species responsible for high-performance antibiotic degradation.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Shiwen Wu,Yaxin Li,Shuqi Wang,Hongzhi Lu,Shuzhen Zheng,Shuai Sun,Shoufang Xu
Herein, a method was developed for triple emission ratiometric fluorescence/colorimetric dual-mode detection of glutathione (GSH) based on multifunctional copper-doped carbon dots (Cu-CDs). Cu-CDs displayed dual emission at 465 nm and 650 nm, along with oxidase-like (OXD) and peroxidase-like (POD) activities. The Cu-CDs effectively catalyzed the oxidation of o -phenylenediamine (OPD) to 2,3-diaminophenazine (DAP), which emitted yellow fluorescence at 550 nm and selectively quenched the blue fluorescence of Cu-CDs at 465 nm through an inner filter effect (IFE), while the red fluorescence at 650 nm unaffected. GSH could effectively chelate Cu 2+ in the Cu-CDs, inhibiting DAP formation, led to the recovery of the 465 nm fluorescence, enabling ratiometric fluorescence detection of GSH. The triple-emission system enabled the discrimination of structurally similar substances while improved the sensitivity of the detection. The fluorescence detection demonstrated a limit of detection (LOD) of 1.13 μM for GSH. For colorimetric detection, Cu-CDs catalyzed H 2 O 2 -mediated 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue product (oxTMB) with absorption peak at 652 nm, which further can be reduced by GSH to colorless TMB. Thus, the colorimetric detection of GSH was achieved with LOD of 3.78 μM. The recoveries of the two methods for detecting GSH in fruits and vegetables were found to be 89.67–106.32 % with RSDs as low as 0.6 % (fluorescence) and 1.2 % (colorimetric), respectively.
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CATALYSIS LETTERS
·
2025
·Vol. 155
·期 9
·1-12
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IF 2.4
Zhong Yuhang,Xu Hui,Li Zhiguo,Zhang Yuqi,Hou Jianyuan,Yuan Yuan,Liu Xingang,Zhang Renxi
This study demonstrates an innovative approach for green and efficient hydrogen production from seawater, utilizing Cu–Fe/Al 2 O 3 catalysts coupled with a dielectric barrier discharge (DBD) system. The catalytic performance was systematically evaluated under varying voltages and Cu/Fe ratios in a DBD reactor to optimize hydrogen production efficiency. Subsequent investigations focused on humidity and gas flow rate effects using the optimal catalyst (Cu/Fe/Al 2 O 3 = 2:1:9). Multimodal characterization (XRD, XPS, FTIR, OES) combined with DFT calculations revealed the mechanistic role of Cu–Fe/Al 2 O 3 in plasma-driven hydrogen generation. The synergistic interaction between Fe and Cu enhanced hydroxyl/hydrogen radical generation through improved adsorption, thereby boosting hydrogen evolution efficiency and yield. The optimized system achieved a maximum hydrogen yield of 7.4 g/kWh at 3 kV operating voltage, 3 L/min argon flow, and 100% relative humidity, establishing an energy-efficient pathway for sustainable hydrogen production from seawater. Graphical
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Food Bioscience
·
2025
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IF 5.9
Xueer Lin,Jiahui Zou,Guancheng Zhu,Linfeng Pang,Yuqi Chen,Yanyan Huang
To effectively overcome the shortcomings of traditional chromatographic and enzymatic methods for detecting organophosphorus pesticides, Cu-MOFs with inherent peroxidase (POD)-like activity were constructed for direct colorimetric detection of omethoate (OMT). Benefiting from the mesoporous and benzene ring structure, Cu-MOFs demonstrated excellent OMT enrichment capability. OMT exhibited a pronounced inhibitory effect on the POD-like activity of Cu-MOFs whereas other pesticides showed negligible interference, demonstrating the specific identification of OMT by Cu-MOFs. Experimental data and theoretical calculations indicated that OMT effectively bond to Cu-MOFs through Cu 2+ coordination and ligand binding. Owning to its enrichment, identification and catalytic abilities, OMT detection was achieved and the limit of detection (LOD) was 16 μg L −1 . Furthermore, a smartphone-assisted portable detection was established with a LOD of 79 μg L −1 . Validation experiments of cabbage samples confirmed its’ reliability for practical applications. Our system integrated the enrichment, identification, and detection of OMT within a single system, improving both sensitivity and operational convenience.
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MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Jijun Guan,Liwei Wang,Fugang Xu,Guangran Ma
Ratiometric electrochemical probes are vital for reliable sensing but often suffer from complex preparation, reference molecule leakage, and single function. To address these limitations, this study reports a flower-like Cu-doped zeolite imidazolate framework (Zn 0.5 /Cu 0.5 /Co-ZIF) prepared at room temperature via simple stirring. This material acts as both an electrode material and a robust ratiometric probe for detecting catechol (CC) and acetaminophen (AP). Compared with pristine Zn/Co-ZIF, Cu doping confers several advantages: transforming lamellar Zn/Co-ZIF into a flower-like structure for enhanced electron and mass transfer; significantly improving redox current of CC/AP for high sensitivity; and generating a stable Cu-based redox signal as an internal reference, improving accuracy. The sensor achieved wide linear ranges (CC: 0.5–150 μM; AP: 0.2–300 μM) and low detection limits (CC: 0.12 μM; AP: 0.04 μM) for determination of phenol compounds. Compared to single-signal assay, the ratiometric approach showed lower RSDs and superior linear correlations. The sensor also achieved satisfactory simultaneous detection of CC and AP, along with accurate quantification in lake water samples. This work presents a facile strategy for a MOF based reference probe, offering a promising platform for stable and accurate ratiometric electrochemical sensors.
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ACS Applied Bio Materials
·
2025
·
IF 4.7
Zhichao Zhang,Xijing Zhuang,Xufeng Dong,Jingyuan Zhao,Hao Zhang,Hong Zheng,Chang Xu
The moist wound environment of the human body immediately following injury is highly susceptible to microbial colonization and subsequent infection, potentially triggering a prolonged inflammatory response. Existing dressings tend to be more monofunctional, making it difficult to cope with wound bleeding and bacterial growth at the same time. The development of more effective wound dressings is a critical priority in contemporary wound care. In this study, we developed a hydrogel wound dressing comprising two components: (1) antimicrobial particles of curcumin (Cur)-loaded bimetallic organic framework (Cu/ZIF-8), and (2) a carrier hydrogel formed by methacryloylated gelatin (GelMA) cross-linked with methacryloylated hyaluronic acid (HAMA). The experimental results confirmed the successful loading of curcumin with 45.6% for Cu/ZIF-8@Cur. In addition, the material exhibits pH-responsive behavior, excellent biocompatibility (>80% cell survival), low hemolytic activity (<5%), and potent antimicrobial activity (≥99%) against E. coli and S. aureus. It is stable under neutral conditions and releases bactericidal particles under bacterial infection (in an acidic environment) to achieve sterilization. This study addresses the limitations of gelatin-based dressings, such as inadequate hemostatic capacity and weak antimicrobial performance, and it demonstrates promising potential for clinical translation.
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Environmental Science-Nano
·
2025
·
IF 5.1
Yihui He,Lin Ye,Wanglai Cen,Jianjun Li,Dengrong Sun
The effective elimination of the highly toxic phosphine (PH3) is crucial for environmental and human health. Nonetheless, developing efficient methods for the removal of PH3 and its conversion into valuable resources remains a significant challenge. Herein, we reported a novel strategy of N doping in Cu-doped active carbon (Cu-AC) to realize efficient PH3 removal. Introduction of N into Cu-AC induces strong interaction between Cu and N, which greatly promotes the dispersion of Cu species and stabilize the active Cu2+ species of PH3 removal due to the electronegativity of N. In addition, doping N also improves the basic intensity and oxidation capability, which provide high benefits for the adsorption and activation of PH3. As a result, the obtained N-doped Cu-AC (Cu-N-AC) shows exceptional performance for the oxidative removal of PH3 to generate H3PO4 at 70 °C, reaching a high breakthrough capacity of 534.5 mg·g-1, which outperforms most of the previously reported catalysts. The Cu-N-AC can be easily regenerated through water washing and air drying, showcasing its great potential for practical applications. This study not only introduces a promising material for PH3 removal, but also offers an innovative approach to design catalysts for efficient PH3 utilization, presenting an important contribution to the field of phosphorus resource recovery.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Hailun Geng,Shengying Zhao,Minghui Tan,Jie Yao,Hao Song,Chuang Gu,Tiecheng Shang,Wenzhong Shen,Guohui Yang,Noritatsu Tsubaki
Metal-zeolite catalysts are widely utilized in various industrial reactions. However, the size, loading, and reduction of metal species are interdependent, complicating efforts to optimize their catalytic performance. In this study, we introduce a novel strategy for the synthesis of metal-zeolite catalysts, termed the multi-amino effect. This new method involves functionalizing zeolite surfaces with aliphatic diamines, such as 1,3-diaminopropane, to leverage the multifaceted properties of the amino group. 1,3-diaminopropane is anchored to the zeolite framework via one terminal amino group, while the other terminal amino group facilitates the efficient loading and dispersion of Cu species through interaction with Cu 2+ . The multi-amino groups present in the structure enable the in situ multi-stage release of abundant NH 3 during calcination, facilitating the self-reduction of Cu species to generate a high proportion of Cu + and Cu 0 species. Notably, the Cu-MOR catalyst prepared by this approach exhibits a significantly improved methyl acetate production rate, which is 1.56 times higher than that of the traditional hydrogen reduction method and 1.37 times greater than that of the ammonia pool method, owing to the abundance of highly dispersed and highly active Cu sites (Cu + :Cu 0 ≈ 1:1). This multi-amino effect strategy will help to develop more efficient and tunable metal-zeolite catalysts, driving advances in industrial processes such as sustainable chemical production and energy conversion, with significant improvements in both catalytic activity and cost-effectiveness.
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Frontiers in Pharmacology
·
2025
·
IF 4.8
Sitong Lai,Kunhui Sun,Yun Wu,Xueyuan Wu,Yiqi Yan,Guojing Liu,Xiaoyi Liu,Yuanyuan Ge,Lina Zeng,Ziyu Guo,Shuhong Wang,Ping Wang,Bing Wang,Han Zhang,Xie-an Yu
Introduction: The procedural complexity and time-consuming of conventional pesticide residue detection methods in traditional Chinese medicines (TCMs) significantly impeded their application in modern systems. To address this, this study presented an innovative dual-mode sensor driven by Cu2+/Cu+ redox-cycling, which achieved efficient signal transduction from enzyme inhibition to optical response for rapid acetylcholinesterase (AChE) activity and organophosphorus pesticide (OP) residue detection.Methods: The AB-Cu NPs sensor, a dynamic redox-responsive system, was constructed via coordination-driven assembly of Azo-Bodipy 685 (AB 685) and Cu2+. Initially, Cu2+ quenched the optical signals of AB 685 through photoinduced electron transfer (PET), maintaining an “OFF” state. Upon AChE-catalyzed hydrolysis of acetylthiocholine chloride (ATChCl) to thiocholine (TCh), Cu2+ was reduced to Cu+, thereby activating dual ultraviolet-visible (UV-Vis)/fluorescence (FL) signals (“ON” state). OP residues were quantified by their inhibition of AChE, which blocked Cu2+/Cu+ conversion and suppressed signal generation.Results: The sensor exhibited positive sensitivity with detection limits of 0.0327 U/L for AChE activity and 1.72 ng/mL for triazophos, leveraging Cu2+/Cu+ redox-cycling for signal amplification. Notably, the Cu2+/Cu+ valence interconversion coupled enzyme inhibition with probe responsiveness, ensuring the both procedural easily and rapid response.Discussion: This study developed a portable detection platform based on valence interconversion coupled enzyme inhibition with probe responsiveness. The dual-signal output enhanced reliability and the redox-driven mechanism enabled signal amplification. This advancement provided a real-time, portable and rapid detection for AChE activity and pesticide exposure of TCMs, bridging gaps in agricultural safety and pharmaceutical standardization.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Lijun Wang,Jing Cao,Zhenni Yu,Haixia Hu,Xiao Yang,Xianggui Chen
The newly established lower maximum residue limit (MRL) for thiamethoxam (TMX) necessitates the development of more sensitive detection methods. This study presents a novel colorimetric-fluorescence sensing platform to detect TMX through graphene quantum dot (GQD)-mediated regulation of Fe 3 O 4 @Cu@PLL nanozyme activity. The mechanism investigation revealed that GQDs effectively suppress the peroxidase-like activity of Fe 3 O 4 @Cu@PLL by reducing and scavenging hydroxyl radicals (•OH) generated during catalytic reactions, which constitutes the fundamental principle of the dual-mode detection strategy. The sensing system was constructed through the hybridization of aptamer-functionalized Fe 3 O 4 @Cu@PLL (Fe 3 O 4 @Cu@PLL/Apt) with complementary DNA strand-modified GQDs (CS-GQDs), forming Fe 3 O 4 @Cu@PLL/Apt-CS/GQDs complexes. Following magnetic separation, two distinct detection channels were established: (1) The supernatant showed negligible fluorescence due to CS-GQDs removal, while (2) the precipitates exhibited suppressed peroxidase-like activity owing to GQD-induced inhibition. However, TMX presence triggered the dissociation of CS-GQDs from the complexes through competitive binding with the aptamer. This resulted in two measurable signals: a fluorescence signal recovery at 450 nm in the supernatant containing CS-GQDs, and restored nanozyme activity in the Fe 3 O 4 @Cu@PLL/Apt+TMX precipitates that catalyzed H 2 O 2 -mediated oxidation of TMB to blue-colored oxTMB. The optimized system achieved detection limits of 8 ng/mL (colorimetric) and 6 ng/mL (fluorescence), demonstrating high sensitivity. Validation experiments using three spiked samples (tea, beans, and kumquats) showed satisfactory recoveries ranging from 94.59 % to 108.23 %, confirming the method's reliability for accurate, on-site TMX detection in food matrices.
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