LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
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IF 6.6
Shudong Peng,Jiajun Li,Yating Long,Ying Zheng,Dongming Lan,Yonghua Wang
Foodborne Escherichia coli is one of the health-threatening pathogens existing on the surface of vegetables and fruits. Although antibiotics are the first line of defence against bacteria, increasing concerns are awake about the multidrug resistance caused by overuse and misuse of antibiotics. Therefore, alternative antibacterials are urgently necessary. In this context, we identified, cloned and expressed a novel endolysin LysD6E derived from marine phage D6E for its potential antibacterial action against E. coli . LysD6E consists of an Amidase_3 domain belonging to the N-acetylmuramoyl-L-alanine amidase family. LysD6E was stabile between 4 and 70 °C, and pH between 6 and 10. It had strong lysogenic activity against E. coli and Salmonella typhi . Furthermore, when applying 0.5 μM of LysD6E together with 5 mM EDTA on lettuce for 2 h, viable counts of E. coli ATCC 25922 reduced by 2.05 Log CFU/mL. Our findings suggest that phage-derived LysD6E has the potential against foodborne E. coli in raw vegetables.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Sirui Liu,Qianjin Ye,Qin Li,Zhanzhen Ma,Ran Yang,Di Li,Deli Jiang
Carbon nitride (CN) has received extensive interest due to their good stability and unique conjugated structures. However, the weak charge separation ability and poor active sites lead to undesirable photocatalytic CO 2 reduction activity. Here, we successfully prepare all-organic PTA/V C -CN S-scheme heterojunction photocatalysts by integrating CN with carbon vacancies (V C -CN) and perylene tetracarboxylic acid (PTA) for photocatalytic CO 2 reduction. The introduced carbon vacancies can act as electron trapping sites, break the symmetry structure of CN and facilitate in-plane photogenerated carrier separation and transfer of CN, while the coupling of PTA with V C -CN via π-π interaction to form a built-in electric field further speeds up the interfacial charge transfer, thereby collectively enhancing the separation and transfer efficiencies of photogenerated carriers. Moreover, the high reduction capability of V C -CN with carbon vacancies as possible active sites and wide-spectrum light absorption of PTA render the S-scheme heterojunction with high photocatalytic activity. The optimum PTA/V C -CN photocatalyst exhibits a high CO production rate of 47.91 μmol g −1 h −1 , which is about 10.7 and 4.2 times higher compared to those of CN and V C -CN, respectively. This work provides a new strategy for developing all-organic CN-based S-scheme heterojunction photocatalysts used in CO 2 conversion.
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Ziyi Wang,Di Wang,Lily Chen Wang,Huilan Chen,Yunyan Li,Weihong Zheng,Jizhen Shang,Limin Lu,Guojiang Mao,Yuchun Qiao,Hua Wang
In this article, gold (Au) and silver (Ag) nanoparticles (NPs) were synergistically grown into flower-like covalent organic frameworks (COFs) through a chemical reduction method. It was discovered that COF-Au could present the obvious oxidase-like catalysis, which could decrease after adding silver elements to yield COF-Au-Ag. To our surprise, the catalytic activities of COF-Au-Ag were obviously enhanced with mercury (II) (Hg 2+ ) by forming Au-Ag-Hg alloys, which was 7.0 and 2.5-folds higher than those of COF-Ag and COF-Au separately with Hg 2+ , respectively. A COF-Au-Ag catalysis-based colorimetric method was then developed for the turn-on detection of Hg 2+ ions in fish muscle samples, providing a linear detection range from 0.0010-2.0 mg/kg. Moreover, the as-fabricated COF-Au-Ag could selectively absorb Hg 2+ ions through forming stable coordination complexes. Importantly, it could facilitate Hg 2+ ions to be eliminated from the aquaculture tailwater with the removing efficiency up to 99.61 % and adsorption capacity of 200.59 mg/g, which is significantly higher than that of COF, highlighting the superior adsorption efficiency of COF-Au-Ag. Such a COF-Au-Ag-based colorimetric detection and removal method for Hg 2+ ions may promise extensive applications in the Hg 2+ -toxic disease diagnosis, environmental monitoring and aquaculture food safety fields.
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Foods
·
2025
·Vol. 14
·期 12
·2142
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IF 5.1
Zile Wang,Dongdong Chen,Xiaomei Zheng,Yuqing Li,Shaoqian Jiang,Yanfei Chen,Jingjian Jia,Libo Yu,Tao Peng
Staphylococcus aureus(S. aureus) poses a significant threat to public health and safety, and enhancing the monitoring ofS. aureusin food is essential to curb and prevent foodborne transmission. In order to obtain strains for more convenient and rapid use in quality control or quantitative analysis, this study designed a ready-to-use “ball-in-ball” microsphere based on a novel cryoprotectant combined with drop freeze-drying technology. When using a cryoprotectant that contains 1.5% bovine serum albumin, 4.5% trehalose, 8.2% polyethylene glycol 8000, and 4.1% D-mannitol, the survival rate ofS. aureuscan reach 98.2 ± 2.6%. This cryoprotectant effectively preventsS. aureusfrom shrinking, deforming, and damaging cell walls. Additionally, it shows desirable protective efficiency for other Gram-positive bacteria. The molding of microspheres is efficient and cost-effective, demonstrating good uniformity and stability without the need for pre-freezing. The moisture content and the count ofS. aureusshowed no significant changes over 90 days at −20 °C. In the simulated contaminated sample, the recovery rate ofS. aureusin milk and green tea was 83.1–93.7%. This study could provide a practical approach to improve the monitoring efficiency ofS. aureusand shows potential as a generalized strategy for preparing ready-to-use strains related to food safety.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Naying Zhang,Zefeng Ren,Dongsheng Wei,Meng Yang,Meng Niu,Chunxiao Shen,Xuanyan Jin,Maolin Wei,Jaehwan Choi,Myeongsam Park,Zhengqun Li
Superoxide dismutases (SODs) are essential enzymes that protect cells by converting harmful superoxide radicals into oxygen and hydrogen peroxide. However, their application in industrial and medical settings is often limited by poor stability under extreme conditions such as high temperatures, extreme pH, and oxidative stress. In this study, we identified and characterized two highly stable manganese-dependent SODs from extremophiles. These enzymes remained highly active at temperatures up to 80 °C, across a broad pH range (3.0–12), and in the presence of oxidative agents and denaturing chemicals. Structural analysis revealed that their exceptional stability is attributed to tightly folded structures, strong hydrophobic cores, and robust metal-binding sites. Both Os SOD and Gt SOD demonstrated robust catalytic performance, with k ca t k m values of 2.29 × 10 7 and 1.06 × 10 7 M −1 ·s −1 , respectively. In cell-based models, they effectively reduced oxidative stress and inflammation, indicating potential for antioxidant therapy. Additionally, their long-term stability supports their use in cosmetic and pharmaceutical formulations. Overall, these extremophile-derived SODs are highly stable and efficient enzymes with broad potential for industrial, medical, and cosmetic applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Yuhan Zhao,Xiaojie Zhou,Mei Li,Xinghui Qiu
Aedes albopictus is a primary vector of arboviral diseases such as dengue fever, chikungunya fever, and Zika. The rapid development of insecticide resistance is becoming a major challenge to sustainability of insecticide-based interventions for the control of Ae. albopictus borne arboviral diseases. Understanding the molecular basis of resistance is useful for developing effective insecticide-based control action. Our recent study showed that overexpression of the AalbCYP9J26 gene was associated with insecticide resistance in field Ae. albopictus populations in Beijing, but its contribution to insecticide metabolism and resistance remains unknown. In this study, the metabolic capacity of AalbCYP9J26 towards six insecticides was examined using an enzyme system reconstituted with Escherichia coli -produced AalbCYP9J26 protein and its redox partners (AalbCPR and Aalbb5). The results showed that AalbCYP9J26 was able to metabolize temephos, malathion, propoxur, permethrin, and deltamethrin, while no significant activity against bendiocarb was observed. Moreover, the Drosophila GAL4/UAS transgenic system confirmed that enhanced expression of AalbCYP9J26 conferred increased tolerance to malathion, permethrin, deltamethrin, and propoxur. This study demonstrates that AalbCYP9J26 plays a critical role in insecticide metabolism and resistance, suggesting AalbCYP9J26 may serve as a valuable biomarker for monitoring insecticide resistance and a target for new insecticidal agents.
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BRITISH JOURNAL OF ANAESTHESIA
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2025
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IF 9.2
Zhenxing Li,Xiaoling Huang,Jiahui Ma,Qianxi Liu,Li Chen,Xing Liu,Qian Qin,Hui Yang,Rui Han,Kuankuan Li,Rong Hu,Xuebin Yan,Yuzhao Huang,Dong Huang,Haocheng Zhou
Background Spontaneous pain is commonly reported in patients with nerve injuries, posing a great challenge to physicians. However, the mechanisms underlying spontaneous pain remain largely unknown in comparison to evoked pain (e.g. allodynia and hyperalgesia). Cortical processing is vital in coding nociception, and dysfunction of anterior cingulate cortex might facilitate pain hypersensitivity. This indicates a distinct pattern of ensemble activity during spontaneous pain-like behaviour. Methods Combining in vivo calcium imaging and ex vivo electrophysiological recording, we explored the maladaptive changes of cortical neurones at individual and population levels induced by several models of neuropathic pain in mice. We also examined the role of the anterior cingulate cortex in the regulation of spontaneous pain-like behaviour using optogenetic methods. Results We identified a common status of cortical hyperactivity in pyramidal neurones and neural ensembles recorded in the anterior cingulate cortex. Optogenetic inhibition of the anterior cingulate cortex inhibited spontaneous pain-like behaviour, while optogenetic activation restored it. In addition, the cortical representation of spontaneous behaviour was inhibited by nerve injury, which conversely was restored with pain relief. In freely moving animals, we further demonstrated a stable mode of cortical integration in response to spontaneous grooming episodes, independent of the presence of pain, with a dynamic reconfiguration mechanism. Conclusions Our data reveal an essential role of the anterior cingulate cortex in the processing and modulation of non-evoked reflex in distinct preclinical models of neuropathic pain in mice.
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Research
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2025
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IF 10.7
Chengjunyu Zhang,Sen Liu,Jianhui Zhang,Junlin Lu,Zehua Chen,Bolin Pan,Chu Liu,Ming Huang,Hengji Zhan,Hongjin Wang,Siting Chen,Kaiwen Jie,Baoqing He,Jingdie Wu,Ye Li,Haifeng Wang,Jing Zhao,Qiang Zhang,Xu Chen
Ferroptosis has promising potential for augmenting antitumor effects, but monotherapy with ferroptosis inducers in vivo has been reported to have limited efficacy in tumor management. The development of synergistic strategies with targeted capabilities is crucial for enhancing the antitumor efficacy of ferroptosis inducers. In this study, we designed and characterized a novel self-assembled nanomedicine by mixing ferrous ions (Fe 2+ ) and epigallocatechin gallate (EGCG) in a controllable manner and encapsulating the ferroptosis inducer RSL3, named Fe-EGCG@RSL3. This multifunctional nanomedicine effectively induces ferroptosis and growth inhibition in bladder cancer cells and patient-derived organoids. In vivo, Fe-EGCG@RSL3 was enriched in the subcutaneous tumors of allogenic and xenograft mouse models, thereby substantially overcoming RSL3 resistance. Intravesical instillation of Fe-EGCG@RSL3 controls orthotopic bladder tumor progression. Furthermore, nanomedicine potentiates the therapeutic effect of anti-programmed cell death protein 1 (PD1) immunotherapy by increasing the cytotoxicity of CD8 + T cells to cancer cells and modulating the proportions of both T-cell and myeloid cell subpopulations within the tumor immune microenvironment. Overall, Fe-EGCG@RSL3 has dual functions as a multifaceted nanomedicine that integrates ferroptosis induction with immunomodulation, offering a novel and clinically translatable strategy for bladder cancer therapy.
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BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
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2025
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IF 4.2
Dejian Chen,Yu Wang,Xiaomin Wang,Xiaosa Si,Yajing Weng,Shengbing Liu,Weiwei Pan,Ying Xu,Yong Wang,Yaling Zhang
Ferroptosis is a type of cell death caused by iron-dependent lipid peroxidation and excessive production of reactive oxygen species (ROS). Hyperandrogen exposure can induce ovarian ferroptosis in polycystic ovary syndrome (PCOS). However, there is not enough direct evidence regarding ovarian ferroptosis in PCOS to prove the role of ferroptosis in PCOS and the underlying mechanism. Nuclear receptor coactivator 4 (NCOA4) expression in ovarian granulosa cells and its mechanism of mediating ferroptosis have not been studied. In this study, we aimed to investigate how iron overload in ovarian granulosa cells and hyperandrogen exposure-induced ferroptosis affect ovarian development in a PCOS model. Increased ferroptosis was observed in hyperandrogenic mice with PCOS and in 5α-dihydrotestosterone (DHT)-induced primary granulosa cells. Iron deposition increased significantly, followed by increased cellular Fe 2+ concentration, whereas ROS accumulation, malondialdehyde (MDA), glutathione (GSH), and the glutathione/oxidized glutathione (GSH/GSSG) ratio decreased. NCOA4 and transferrin receptor expression increased; however, ferritin heavy chain 1 expression decreased in ovarian tissues. Androgen receptor and NCOA4 showed increased colocalization in the ovarian granulosa cell layer. Notably, DHT induced ferroptosis by activating NOCA4-dependent ferritin autophagy. However, deferoxamine mesylate inhibited ferroptosis in the ovarian granulosa mice cells, ameliorating the PCOS phenotype. Steroid hormone biosynthesis, arachidonic acid metabolism, and unsaturated fatty acid pathways were most significantly affected, providing a reference for PCOS diagnosis and treatment and corresponding drug development.
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DALTON TRANSACTIONS
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2025
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IF 3.3
Jie Jin,Haoran Li,Yunqi Xu,Qunling Fang,Xia Chen,Weili Kong,Shouhu Xuan
Pathogenic microorganisms and recalcitrant pollutants in water present a persistent and severe threat to human health. Consequently, there is an urgent need to develop an integrated solution that not only catalyzes the efficient degradation of pollutants but also exhibits potent antibacterial properties. In this study, Fe3O @AuPd/PDA4 nanocomposites were synthesized via in situ redox polymerization. Owing to their active catalytic components and superior magnetic recyclability, the material can be used as an efficient and easily recycled catalyst. In the presence of NaBH4, the material exhibited excellent catalytic activity for the reduction of 4-nitrophenol, with a reaction rate constant of 1.07 min-1 mg-1. Furthermore, the material demonstrated excellent cyclic stability. After 6 cycles, the catalytic conversion rate remained above 95%. Under near-infrared (NIR) light irradiation, the reaction rate can be increased to 3.47 min-1 mg-1. Similar NIR-enhanced catalytic effects were observed for 2-nitrophenol and 3-nitrophenol. In addition to its catalytic properties, the material also demonstrates excellent NIR photothermal response. This characteristic renders it highly effective in eliminating both Escherichia coli and Staphylococcus aureus. Under the synergistic influence of NIR light and an external magnetic field, the antibacterial efficacy is markedly enhanced. This multimodal antimicrobial mechanism offers a promising approach for developing innovative antimicrobial strategies.
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ANALYST
·
2025
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IF 3.3
Sunying Yang,Jiaqi Cai,Liu Su,Jingxiao Huo,Huadong Xu,Yuhao Gu,Changhe Wang,Hetong Qi,Honglan Qi
We fabricate gold disk microelectrode (Au DME) and develop a label-free electrochemical aptasensor for highly sensitive and selective detection of dopamine (DA) in a brain slice by using anti-DA specific aptamer as molecular recognition element and DA oxidation signal as analytical signal. The Au DME with a disk-shaped geometry and a radius in range from 1.25 to 4 μm is fabricated by fine tuning of the size of gold microwire inside a borosilicate capillary with laser-assisted pulling and mechanical polishing method for easily positioning the target object. A label-free electrochemical aptasensor is fabricated by self-assembling an anti-DA specific aptamer on the surface of Au DME with a radius of 2 μm. The obtained aptasensor can directly detect DA based on the oxidation current of DA as analytical signal, in which the recognition of DA by the anti-DA specific aptamer immobilized on Au DME allow DA close to the electrode surface and facilitate the electrochemical oxidation of DA. Taking advantage of Au DME with a small capacitive current and anti-DA specific aptamer with good binding affinity to DA, the label-free electrochemical aptasensor not only can sensitively detect DA with a wide linear range of 0.5 to 27 μM and a low detection limit of 0.11 μM, but also can selectively detect DA in the presence of other interference neurochemicals. Moreover, the label-free electrochemical aptasensor is successfully applied in the recording of the dynamic increase of DA in a brain slice from mice upon electrical stimulation. This work provides a promising strategy for the preparation of label-free electrochemical aptasensor based on microelectrode with high spatial resolution, sensitivity and selectivity to determinate neurochemicals dynamics in living systems.
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JOURNAL OF CONTAMINANT HYDROLOGY
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2025
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IF 4.4
Zengze Liu,Ziwei Zheng,Yanlin Liu,Xiaoqiu Yang,Yangwei Chen,Fang Tan
This study featured about the synthesis of graphene oxide hydrogel-CuO@reduced graphene oxide (rGH-CuO@rGO) micromotors with core-shell structure via an ultrasonication-assisted chemical precipitation method and their application for catalytic dye degradation. The micromotors were comprehensively characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), spectroscopic techniques (Fourier transform infrared (FTIR), Raman), surface area measurement (BET), and X-ray photoelectron spectroscopy (XPS) to determine their microstructure, composition, and chemical states. Degradation performance for methylene blue (MB) was evaluated under varying conditions: reaction time (0–120 min), peroxymonosulfate (PMS) concentration (0–5 g·L −1 ), pH (3–8), quantity of micromotors (12–24), and MB concentration (5–100 mg·L −1 ). The removal rate of MB was achieved over 90 % within 120 min at pH 8. The micromotors demonstrated excellent catalytic efficiency, reusability and stability over four cycles. In the dual oxidant system (H₂O₂/PMS) the micromotors exhibited effective self-propulsion and stable MB removal. The generation of hydroxyl (•OH) and sulfate (•SO₄ − ) free radicals in the system was confirmed by electron paramagnetic resonance spectrometer (EPR). Ultra High Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-QTOF/MS) was used to identify the degradation intermediates, and the possible MB degradation pathways were proposed. Moreover, the micromotors showed satisfactory performance in removing mixed dye wastewater and phenolic compound. Additionally, the low ecotoxicity of the micromotors was demonstrated by cytotoxicity assessment. This study provided a novel and valuable route to synthesizing self-driving micromotors with potential extensive applications in environmental remediation.
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危险品化学品经营许可证（带存储）