INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Yuedan Xu,Xinni Pan,Dongni Shen,Yi Sun,Wei Liu,Ye Lin,Baiping Fu,Ling Zhang
Dentine hypersensitivity (DH) is a common oral health issue and occlusion of the exposed dentinal tubules (DTs) is regarded as the most effective therapeutic treatment nowadays. However, it is still difficult to develop easy and effective strategies for deep occlusion of DTs. In this study, we develop a strategy for occluding DTs deeply and compactly via simple application of occlusion media including (poly-L-aspartic acid)‑strontium (PAsp‑strontium) and phosphate/fluoride. The bonding of strontium ions to poly-L-aspartic acid formed a positively charged PAsp‑strontium complexes. After application of 15 min each, the PAsp‑strontium and phosphate/fluoride rapidly penetrated into the DTs in turn via the electrostatic interaction, then occluded the DTs with crystals up to a depth of 150 μm. The occlusion within DTs was resistant to abrasive and acidic challenges. The occlusion media performed better than commercial desensitizers Duraphat and Gluma. Moreover, this strategy possessed sufficient biocompatible and excellent performance in vivo. The application of occlusion media would shed light on in the management of DH.
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高影响因子
LANGMUIR
·
2023
·
IF 3.9
Haoran Li,Daqing Chen,Wen Zhou,Donghao Cheng,Danhua Ge,Xiaojun Chen
Monitoring l-cysteine (l-Cys) is of importance for human health and food safety. Herein, we designed a novel strategy for bimetallic Au and Ag/AgCl anchoring on Ni-doped ZIF-67 to form core–shell nanocubes (Ni-ZIF-67/AuAg/AgCl) using the galvanic replacement processes. The unique properties of ZIF-67 nanocubes were conducive to generating strong synergistic catalytic effects with Au and Ag/AgCl, particularly when Ni-ZIF-67/AuAg/AgCl composites were employed as oxidase mimics for catalyzing the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). The Ni-ZIF-67/AuAg/AgCl composites displayed strong affinity toward TMB, displaying a lower Michaelis constant Km value of 0.25 mM and a higher maximum initial rate Vmax of 9 × 10–8 M s–1. By virtue of the nanozyme, the colorimetric sensor was constructed for l-Cys detection with a relatively low detection limit of 0.051 μM. The superior catalytic performance of the as-prepared Ni-ZIF-67/AuAg/AgCl composites can be ascribed to the core–shell structure, large specific surface area, and strong synergistic catalytic effects, which are beneficial for exposing more active sites and enhancing the conductivity to further boost their catalytic activity.
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JOURNAL OF ETHNOPHARMACOLOGY
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2023
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IF 5.4
Jiangyan Rao,Hongpu Li,Haonan Zhang,Xiaoxia Xiang,Xinyu Ding,Li Li,Funeng Geng,Hongyi Qi
Ethnopharmacological relevance Periplaneta americana (L.) (PA) has been used in traditional Chinese medicine for thousands of years for the effect of invigorating blood circulation and removing blood stasis . Modern pharmacological research shown that PA extract exhibits promising effects in promoting wound healing and regeneration, as well as in brain diseases such as Parkinson's disease (PD). However, whether it is effective for neuroregeneration and neurological function recovery after stroke still unknown. Aim of the study This study aims to investigate the potential effect of PA extract to promote brain remodeling through the activation of endogenous neurogenesis and angiogenesis, in addition, preliminary exploration of its regulatory mechanism. Methods Firstly, BrdU proliferation assay and immunofluorescence (IF) staining were used to evaluate the effect of PA extract on the neurogenesis and angiogenesis in vitro and in vivo . Subsequently, the effects of PA extract on brain injury in stroke rats were assessed by TTC and HE. While mNSS score, adhesive removal test, rota-rod test, and morris water maze test were used to assess the impact of PA extract on neurological function in post-stroke rats. Finally, the molecular mechanisms of PA extract regulation were explored by RNA-Seq and western blotting . Results The number of BrdU + cells in C17.2 cells, NSCs and BMECs dramatically increased, as well as the expression of astrocyte marker protein GFAP and neuronal marker protein Tuj-1 in C17.2 and NSCs. Moreover, PA extract also increased the number of BrdU + DCX + , BrdU + GFAP + , BrdU + CD31 + cells in the SGZ area of transient middle cerebral artery occlusion model (tMCAO) rats. TTC and HE staining revealed that PA extract significantly reduced the infarction volume and ameliorated the pathological damage. Behavioral tests demonstrated that treatment with PA extract reduced the mNSS score and the time required to remove adhesive tape, while increasing the time spent on the rotarod. Additionally, in the morris water maze test, the frequency of crossing platform and the time spent in the platform quadrant increased. Finally, RNA-Seq and Western blot revealed that PA extract increased the expression of p-ERK, p-CREB and BDNF . Importantly, PA extract mediated proliferation and differentiation of C17.2 and NSCs reversed by the ERK inhibitor SCH772984 and the BDNF inhibitor ANA-12, respectively. Conclusion Our study demonstrated that PA extract promoted neurogenesis and angiogenesis by activating the CREB/ERK signaling pathway and upregulating BDNF expression, thereby recovering neurological dysfunction in post-stroke.
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 19
·14503
·
IF 5.6
Zhiye Zhao,Ye Li,Shancheng Guo,Yuxu Chen,Haiaolong Yin,Yaxian Li,Guiguang Cheng,Lei Tian
Acute hepatitis (AH) is a common liver disease with an increasing number of patients each year, requiring the development of new treatments. Hence, our work aimed to evaluate the therapeutic effect ofOryza sativaL. indica (purple rice) seed coat on concanavalin A (ConA)-induced AH and further reveal its potential mechanisms. Purple rice seed coat extract (PRE) was extracted with hydrochloric acid ethanol and analyzed through a widely targeted components method. We evaluated the effects of PRE on AH through histopathological examination, liver function, gut microbiota composition, and the intestinal barrier. The potential targets of PRE on AH were predicted by bioinformatics. Western blotting, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay (TUNEL) staining, and corresponding kits were used to investigate PRE effects on predicting targets and associated signaling pathways in AH mice. In AH model mice, PRE treatment increased transformed mouse 3T3 cell double minute 2 (MDM2) expression to inhibit apoptosis; it also markedly downregulated protein kinase C alpha (PKCα), prostaglandin-endoperoxide synthase 1 (PTGS1), and mitogen-activated protein kinase 1 (MAPK1) activity to alleviate inflammation. Thus, PRE treatment also recovered the intestinal barrier, decreased the lipopolysaccharide (LPS) levels of plasma and the liver, enhanced liver function, and improved the composition of intestinal microbiota. In general, PRE targeting MDM2, PKCα, MAPK1, and PTGS1 ameliorated ConA-induced AH by attenuating inflammation and apoptosis, restoring the intestinal barrier, enhancing the liver function, and improving the gut microbiota, which revealed that the purple rice seed coat might hold possibilities as a therapeutic option for AH.Keywords:Oryza sativaL. indica;acute hepatitis;MDM2;PKCα;PTGS1;gut microbiota
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International Journal of Nanomedicine
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2023
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IF 8
Dongmei Guo,Yue Huang,Kun Wang,Chenguang Yang,Lili Ma,Yu Zhang,Haiyang Yu,Manhua Cui,Zhaohui Tang
Purpose Cervical cancer (CC) is a highly vascularized tumor with abundant abnormal blood vessel, which could be targeted by therapeutic strategies. Poly(L-glutamic acid)-g-methoxy poly(ethylene glycol)/combretastatin A4 (CA4)/BLZ945 nanoparticles (CB-NPs) have shown great potential as nano vascular disrupting agents (VDAs) in the realm of synergistic cancer therapy.Methods In this study, we investigated the nanocharacteristics of CB-NPs, focusing on active pharmaceutical ingredients (API), as well as lyophilized samples combining API with protective agents (PAs). The in vivo efficacy of final sample (API + PAs) was evaluated.Results The assembled sphere of API with complex core and thin-shell structure was confirmed. PAs were found to significantly influence in vivo efficacy. Collaborative efforts between API and PAs, namely mannitol and lactose, resulted in the most promising lyophilized sample, ie, the final sample (FS2) for CC therapy. Impressively, FS2 demonstrated an exceptional 100% cure rate on the CC U14-bearing mice model.Conclusion FS2 has provided significant insights for cervical cancer therapy. It is also crucial to develop a comprehensive evaluation strategy for the formulation of nanomedicine, which has the potential to serve as a guideline for future clinical trials.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 413
·期 15
·4013-4022
·
IF 4.157
Liu Chao,Zhao Yunmeng,Xu Di,Zheng Xinxin,Huang Qing
A facile and green approach to the preparation of peroxidase-like nanozymes by reducing and functionalizing graphene oxide (rGO) with Ganoderma polysaccharide (GP) has been achieved in this work. Our results showed that the as-fabricated nanozyme, namely rGO-GP, possessed the excellent property of simulating peroxidase with higher catalytic activity compared with GO or rGO obtained by using chitosan, which may be due to the better dispersion of rGO-GP in the solution. Steady-state kinetics studies further showed that the catalytic process conformed to Michaelis-Menten equation and ping-pong mechanism. Benefiting from the excellent peroxidase property of rGO-GP, we have also successfully established a highly sensitive and selective colorimetric detection approach to trace detection of l -cysteine ( l -Cys). The limit of detection (LOD) of l -cysteine is 0.1 μM and the linear detection range is 2–30 μM. Furthermore, the nanozyme was successfully applied for detecting l -cysteine in serum. This work therefore demonstrates the advantages of rGO-GP as an effective nanozyme in both its green synthesis and detecting application.
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JOURNAL OF MOLECULAR LIQUIDS
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2021
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IF 6.165
Fukang Deng,Kangchun Li,Qing Feng,Kedi Yang,Fuchuan Huang
As the life expectancy increases, so do the wear and damage to joints caused by increased physical activity. In this regard, the development of biofriendly and nontoxic lubricating materials has important social and economic impact. Therefore, this work develops such a lubricant for joints using tea saponin derivative (TSC16) as the bio-lubricating additive to a choline/N-acetyl- l -proline ionic liquid (IL). The TSC16 molecules aggregated in the choline/N-acetyl- l -proline IL to form short fibrous structures, which increased the viscosity of the IL and improved the formation of a lubricating film on the applied area. The fibrous structure of TSC16 enhanced the strength of the lubricating film and improved the lubrication and anti-wear performance. Overall, the developed IL blend exhibits promise for tissue engineering applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Yaoyao Xu,Kehong Hou,Chengcheng Gao,Xiao Feng,Weiwei Cheng,Di Wu,Linghan Meng,Yuling Yang,Xinchun Shen,Yan Zhang,Xiaozhi Tang
To improve the antimicrobial properties of chitosan films, cinnamon essential oil (CEO) nanoemulsion (1% and 3% v/v CEO) stabilized by ethyl-N α -lauroyl- l -arginate hydrochloride (LAE) alone or co-stabilized by LAE and hydroxypropyl-β-cyclodextrin (HPCD) were incorporated into chitosan matrix. The micromorphology , physical and antimicrobial properties of the composite films were compared. The dense structure of the CEO nanoemulsion co-stabilized by LAE and HPCD reduced the water vapor permeability and water content. The incorporation of the CEO nanoemulsion co-stabilized by LAE and HPCD, reduced the adverse effects of CEO on the mechanical properties and microstructure of the film, and even slightly increased the tensile strength . In addition, the antimicrobial properties of chitosan films were enhanced due to the encapsulation and emulsification effect of HPCD and LAE on CEO. This work indicated that the prepared chitosan based edible films had the potential to be used in the field of food packaging to improve food safety.
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高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
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2021
·Vol. 39
·期 8
·966-974
·
IF 3.603
Feng Li-Dong,Bian Xin-Chao,Li Gao,Chen Xue-Si
The effect of exogenous hydroxyl, carboxyl groups and/or Sn 2+ on pyrolysis reactions of poly(L-lactide) (PLLA) was investigated by thermogravimetric analysis (TGA). The activation energy ( E a ) of pyrolysis reactions was estimated by the Kissinger-Akahira-Sunose method. The kinetic models were also explored by the Malek method, and the random degradation behavior was determined by comparing the plots of In{-In[1 - (1 - w ) 0.5 ]} versus 1/ T for experimental data from TGA with model reactions. The pyrolysis reaction rate of PLLA was affected slightly by exogenous hydroxyl and carboxyl groups at lower levels of Sn with 65–70 mg·kg -1 but increased appreciably in the presence of extraneous Sn 2+ , -COOH/Sn 2+ , or -OH/Sn 2+ . The E a values for the pyrolysis reactions of the PLLAs that provided lactide were different under the catalysis of Sn 2+ in different chemical environments because Sn 2+ can form the new Sn-carboxylate and Sn-alkoxide with exogenous carboxyl and hydroxyl groups, which were different in steric hindrance for the formation of activated complex between Sn 2+ and PLLA. Under the catalysis of Sn 2+ , a lactide molecule can be directly eliminated selectively at a random position of PLLA molecular chains, and the molecular chain of PLLA cannot change two PLLA fragments at the elimination site of lactide. However, it was regenerated into a new PLLA molecule with the molecular weight reduced by 144 g·mol -1 .
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
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IF 4.952
Jia-Nan Chen,Xu-Hui Huang,Jie Zheng,Yi-Han Sun,Xiu-Ping Dong,Da-Yong Zhou,Bei-Wei Zhu,Lei Qin
Blue mussel ( Mytilus edulis L.) is a shellfish with high nutritional and economic value. Free amino acids (FAAs), 5′-nucleotides, and lipids that affected the nutritional value and flavor of blue mussels varying with the seasons were analyzed using stable isotope labeling-liquid chromatography coupled with tandem mass spectrometry (SIL-LC-MS/MS) and ultra-performance liquid chromatography-Q-Exactive orbitrap tandem mass spectrometry (UPLC-Q-Exactive Orbitrap-MS). FAAs and 5′-nucleotides were most in the summer. Changes in FAAs between seasons were more obvious than those between genders, except in spring, when females were in the reproductive period. A total of 413 lipids were annotated in blue mussels for one year. Seasonal variation in triacylglycerols (TAGs) was the most evident. TAGs were dominant in females, while phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs) were dominant in males. Overall, blue mussels in summer were more delicious and nutritious. Thus, such findings are of great significance to the seasonal preference of deep processing and consumption of mussel products.
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ANALYTICA CHIMICA ACTA
·
2020
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IF 5.977
Zihan Wang,Li Zhang,Yumin Hao,Wenjuan Dong,Yang Liu,Shengmei Song,Shaomin Shuang,Chuan Dong,Xiaojuan Gong
The fluorescent sensor, especially ratiometric fluorescent sensor, is one of the most important applications for CQDs , which is becoming a research hotspot. Herein, carbon quantum dots co-doped with nitrogen, phosphorus and chlorine (NPCl-CQDs) were synthesized by acid-base neutralization reaction exothermic carbonization method. The as-fabricated NPCl-CQDs could emit blue fluorescence and possess excellent fluorescence properties. Based on the FRET , multifunctional and ratiometric fluorescent sensors for “on-off-on” sequential determination of riboflavin , Ag + , and Cys with good selectivity and high sensitivity were established. The linear range of riboflavin, Ag + , and Cys are 0.50–10.18 μM and 15.89–27.76 μM, 0.66–1.46 mM and 1.50–4.20 mM, and 0.01–0.15 μM and 0.15–0.36 μM with the limit of detection of 3.50 nM, 26.38 μM, and 0.96 nM, respectively. Furthermore, the sensors were successfully used to determine riboflavin, Ag + , and Cys in tablets, river water, and human urine with the recoveries of 95.2–104.0%, 95.6–102.0%, and 94.8–106.4%, respectively. More importantly, the as-constructed “on-off-on” NPCl-CQDs-based ratiometric fluorescent sensors were applied for detecting riboflavin, Ag + , and Cys in HeLa cells with satisfying results. The finding of this study shows the feasibility and effectiveness of the NPCl-CQDs as the available ratiometric fluorescent sensors for the determination of riboflavin, Ag + , and Cys in real samples and living cells.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2020
·
IF 4.972
Halah Aalim,Di Wang,Zisheng Luo
Black rice is recognized for managing diabetes in Chinese folk medicine. Therefore, the present study investigates the effect of thermal treatments and the succeeding cooking on black rice physicochemical properties, phenolic composition, total antioxidant activity (TAA), enzymes and glycation inhibition in addition to starch digestibility. Thermal decomposition of anthocyanin and cyanidin-3-glucoside was evident across all processing methods and reflected in increasing levels of protocatechuic acid, while proanthocyanidins (TPAC) were susceptible to cooking. Roasting of grains sustained total phenolics (TPC), flavonoids (TFC), TPAC, and antilipase activity. Additionally, the combined effect of frying and cooking diminished TFC, TPAC, and α-glucosidase inhibition. The thermally treated grains showed pronounced activity against α-amylase, α-glucosidase, and glycation, whereas their cooked counterparts reduced the estimated glycemic index (eGI), and enhanced resistant starch (RS). Processed grains chrominance, TAA, and apparent amylose content (AAC) showed a significant correlation with phenolics. These findings are demonstrating that black rice processing is favorable for the dietary management of metabolic disorders such as diabetes and hyperlipidemia.
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高影响因子
危险品化学品经营许可证（带存储）