INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Haishan Wu,Jingbo He,Haoran Cheng,Liu Yang,Hyun Jin Park,Jinglei Li
Chitosan-based nanoparticles (CNPs) are widely used in drug delivery, cosmetics formulation and food applications. To accelerate the manufacturing of CNPs, the present study develops a workflow to prepare CNPs in a continuous model. Based on machine learning, the workflow precisely predicts size and polymer dispersity index (PDI) value of CNPs, which impacts on the colloidal stability and applications. Multi-inlet vortex mixer (MIVM) device was fabricated by 3D printing as the reactor. Peristaltic pump was applied to deliver the reaction streams into the MIVM device and produce CNPs by flash nanoprecipitation (FNP) in a continuous way. The developed MIVM device produces CNPs in a controlled manner at a higher output which is promising for upscale applications. Twelve machine learning algorithms were employed to investigate the potential relationship between the reaction independent variables and hydrodynamic characteristics of CNPs. Random Forest, Decision Tree, Extra Tree and Bagging algorithms performed better than other algorithms with the average prediction accuracy around 90 %. The current study demonstrated that supervised machine learning guided FNP using the developed MIVM device is an effective strategy for accurate and intelligent production of CNPs and other similar nanoparticles.
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高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2021
·
IF 6.53
Mariana Carolina Di Santo,Cecilia Luciana D’ Antoni,Ana Paula Domínguez Rubio,Agustina Alaimo,Oscar Edgardo Pérez
Plant-based polyphenols are natural compounds, present in fruits and vegetables. During recent years, polyphenols have gained special attention due to their nutraceutical and pharmacological activities for the prevention and treatment of human diseases. Nevertheless, their photosensitivity and low bioavailability, rapid metabolism and short biological half-life represent the major limitations for their use, which could be overcome by polyphenols encapsulation (flavonoids and non-flavonoids) into chitosan (CS)-tripolyphosphate (TPP) based nanoparticles (NP). In this review, we particularly focused on the ionic gelation method for the NP design. This contribution exhaustively discusses and compares results of scientific reports published in the last decade referring to ionic gelation applied for the protection, controlled and site-directed delivery of polyphenols. As a consequence, CS-TPP NP would constitute true platforms to transport polyphenols, or a combination of them, to be used for the designing of a new generation of drugs or nutraceuticals.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Qunna Xu,Yao Pu,Jiaqi Shi,Xiaoyu Xu,Jianzhong Ma
Natural food preservatives composed of environmentally-friendly, non-toxic, and biodegradable components have gradually become excellent alternatives to traditional food preservatives. This study developed a fully biomass-based, non-toxic, and eco-friendly food preservation material by encapsulating oregano essential oil (OEO) in self-assembled casein (CA)/chitosan (CS) microcapsules crosslinked with tannic acid (TA) via multiple sites. OEO was successfully encapsulated with an efficiency of 92.5 %. Structural analysis revealed that TA crosslinking significantly altered the secondary structure of casein (increasing α-helix content) and enhanced thermal stability. In food-simulating media, the microcapsules exhibited sustained OEO release across four typical environments, confirming their potential for extensive food preservation applications. The microencapsulated emulsion exhibited prolonged antioxidant and antibacterial activities compared with free OEO. Fruit preservation tests verified that the emulsion effectively extended the shelf life of perishable fruits under ambient conditions. Notably, the use of food-grade materials and a TA-mediated multipoint cross-linking strategy not only enhanced microcapsule stability but also offers an innovative approach for developing safe and sustainable food preservatives.
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POLYMER
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2025
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IF 4.5
Haitao Huang,Han Dong,Zhanye Xu,Weijie Tang,Shuhong Lin,Sheng Lin,Lili Zhang,Murat Yilmaz,Linfei Lai
Integrating electroactive materials into mechanically resilient hydrogels remains a major challenge in the development of next-generation wearable electronics. In this work, we introduce a novel strain-induced self-wrinkling strategy to fabricate a multifunctional, sandwich-structured hydrogel electrode composed of polyacrylic acid (PAA), chitosan(CS), and polyvinyl alcohol (PVA). The resulting APC (PAA/CS/PVA) hydrogel demonstrates exceptional mechanical properties achieving a tensile strain of 495% and supporting loads up to 500 g without structural failure. Cryo-polymerization of pyrrole on frozen, pre-stretched hydrogels produced a conformal polypyrrole (PPy) layer on the micro-wrinkled surface of the APC matrix. The APCP hydrogel exhibits a significantly enlarged electroactive interface and enhanced PPy adhesion. APCP-based all-in-one supercapacitor delivers a high areal capacitance of 110.21 mF cm -2 at 0.5 mA cm -2 and power density of 2394.76 μW cm -2 at 5 mA cm -2 . The self-wrinkled surface endows the hydrogel with high strain sensitivity, enabling real-time monitoring of human joint and finger movements. This work provides a scalable and versatile strategy to constructe multifunctional all-in-one hydrogel systems for integrated energy storage and wearable sensing applications.
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Advanced Healthcare Materials
·
2025
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IF 9.6
Linying Yan,Masoumeh Kianfar,Yuqian Liu,Yang Huang,Huining Xiao,Farzad Seidi
The development of injectable hydrogel wound dressings with inherent antibacterial and antioxidant properties for treating full-thickness skin injuries represents one of the most promising solutions in clinical care. In this study, a series of injectable hydrogels with tunable gelation times is designed and prepared through the Diels-Alder (DA) reaction between diene-functionalized quaternary cellulose and maleimide-grafted quaternary chitosan. The high degree of substitution of diene and maleimide groups on these polysaccharides enables the formation of highly crosslinked hydrogels. By fine-tuning the structure of the dienes, the gelation time can be reduced to 4 min at 37 ° C. Both precursors and hydrogels showed strong antibacterial activity with killing efficacies >98% against E. coli and S. aureus . Meanwhile, the encapsulation of epigallocatechin gallate (EGCG, a green tea derivative) endowed the hydrogel with strong antioxidant performance. Moreover, the hydrogel exhibited good biocompatibility with 100% cell viability for NIH3T3 cells. In vivo wound healing assessment in a mouse full-thickness skin defect model revealed that the optimum hydrogel exhibited significant collagen deposition and vascularization, as well as remarkable regenerative wound healing performance, demonstrating its great potential as a bio-based wound dressing for improving wound healing.
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CARBOHYDRATE POLYMERS
·
2025
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IF 12.5
Wei Wan,Rui Zhang,Lingyi Gao,Aijun Jian,Xianbing Xu,Xiaokang Na,Ming Du,Jianyu Zhu,Beiwei Zhu,Chao Wu
The strategic utilization of natural polysaccharides presents a critical approach for enhancing the gelation properties of soy protein isolate (SPI), which is a key factor in developing superior plant-based food textures. This study demonstrates a strategic technique to enhance SPI gel properties via preheating treatment and electrostatic complexation with chitosan oligosaccharide (COS). Structural analyses revealed that preheating and electrostatic complexation induced SPI unfolding, facilitating conformational transitions toward β-sheet (increased from 53.00 % to 65.35 %) and β-turn (increased from 5.10 % to 7.60 %) configurations while exposing buried tryptophan residues. Fourier-transform infrared spectroscopy, surface hydrophobicity, measurement of interaction force, and molecular docking confirmed that SPI-COS complexation was driven by synergistic electrostatic attractions, hydrogen bonds, van der Waals forces, and hydrophobic interactions. Increasing COS concentration (0–0.16 %, w / v ) progressively enlarged complex particle size (100–350 nm) but reduced colloidal stability, as evidenced by turbidity, ζ-potential, and cryo-scanning electron microscopy. Critically, COS incorporation at 0.08–0.16 % ( w / v ) significantly elevated storage modulus (increased by 275 %) and viscosity while optimizing the regulation of bound and immobilized water in a concentration-dependent manner. These findings establish that COS-mediated electrostatic complexation restructures SPI networks into mechanically robust gels, providing a green strategy for next-generation plant-based foods.
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JOURNAL OF CONTROLLED RELEASE
·
2025
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IF 11.5
Mengfan Wang,Xiaoyun Ye,Wanqiong Li,Danhong Chen,Xin Zhao,Peishang Shi,Xinghua Sui,Juan Liu,Feiyu Luo,Xin Yang,Xiaoshuang Niu,Youmei Xiao,Yuzhen Qian,Haoming Ning,Zhuoying He,Huihao Li,Ye Su,Wenxuan Zeng,Guanyu Chen,Yanfeng Gao
The development of oral vaccines faces significant challenges mainly due to the harsh gastrointestinal environment and low antigen-uptake efficiency. The gut-associated lymphoid tissue (GALT), particularly Peyer's patches (PPs), contains abundant immune cells, among which dendritic cells (DCs) serve as professional antigen-presenting cells (APCs) to initiate specific immune responses. In this study, we engineered an orally stable peptide DEBP-8 with high-affinity toward DEC-205, the endocytic receptor which can effectively mediate antigen uptake by intestinal DCs. This stable peptide was then conjugated to chitosan (CS) nanoparticles (CNPs) to design an oral vaccine, OVA@DEBP-8-CNP, encapsulating ovalbumin (OVA) as a model antigen. Harnessing the profiles of chitosan to activate STING pathway, this oral vaccine promotes DC maturation while targeting the endocytic receptor DEC-205 further enhances antigen uptake of the mature DCs. We demonstrated that OVA@DEBP-8-CNP accumulates in intestinal PPs, facilitates antigen cross-presentation to CD8 + T cells, and prevents tumor growth in B16-OVA tumor-bearing mice. The tumor-prevention effects of this oral vaccine are greatly impaired in PPs-deficient mice. To provide additional protection for CNP-encapsulated antigens in gastrointestinal tract, we employed microfluidic technology to coat CNPs within alginate microparticles (ALG MP), further improving the stability of the oral vaccine. This platform highlights the simplified design of DC-targeting oral vaccine for prevention of cancer and other diseases.
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ENVIRONMENTAL RESEARCH
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2025
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IF 7.7
Ziju Yan,Yuchen Liu,Yuyun Chen,Fei Wang
This study presents the synthesis and application of a bifunctional modified magnetic chitosan microsphere (MCS-II) for the simultaneous removal of Cu 2+ and sodium dodecylbenzene sulfonate (SDBS) from electroplating wastewater. The adsorbent was functionalized with polyethyleneimine (PEI) and ethylenediaminetetraacetic acid (EDTA) and embedded with Fe 3 O 4 nanoparticles to enhance adsorption capacity and magnetic separability. Batch adsorption experiments demonstrated that MCS-II exhibited a maximum adsorption capacity of 141.0 mg/g for Cu 2+ (pH = 6, T = 55 °C) with a removal efficiency of 96 %, and a maximum adsorption capacity of 1632 mg/g for SDBS (pH = 2, T = 25 °C) with a removal efficiency of 95 %. Kinetic studies revealed that the adsorption processes for both pollutants followed pseudo-second-order kinetics, indicating chemisorption as the dominant mechanism. Isotherm analysis showed that Cu 2+ adsorption fitted the Langmuir model, suggesting monolayer coverage, while SDBS adsorption exhibited characteristics of both monolayer and multilayer adsorption. Thermodynamic parameters indicated that Cu 2+ adsorption was endothermic (ΔH > 0) and spontaneous (ΔG < 0), whereas SDBS adsorption was exothermic (ΔH < 0) and spontaneous (ΔG < 0). In binary systems, synergistic effects were observed, with SDBS enhancing Cu 2+ adsorption via additional binding sites, while Cu 2+ exerted minimal inhibition on SDBS removal. Characterization techniques (SEM, FT-IR, XPS, etc.) confirmed the successful incorporation of amino and carboxyl groups, which facilitated chelation with Cu 2+ and electrostatic/hydrophobic interactions with SDBS. Moreover, MCS-II demonstrated excellent recyclability and magnetic recovery: after 4 adsorption-desorption cycles, its Cu 2+ adsorption capacity remained at ∼85–92 % of the original, and SDBS removal rate stayed at ∼95 % through 5 cycles, maintaining high adsorption efficiency for practical application. This work provides a promising strategy for designing multifunctional adsorbents to treat complex wastewater containing both heavy metals and anionic surfactants, offering potential for practical environmental remediation applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xuanzuo JIANG,Feifei Feng,Wenyuan JIA,Yuheng YANG,Yongzheng YAN,Zhiqiang CHENG,Yungang LUO,Ji QU,Guomin LIU
Osteoporosis, a systemic bone disease marked by reduced bone mass and deteriorated microstructure, stems primarily from an imbalance between bone formation and resorption. Normally, bone remodeling maintains mass via osteoblast-mediated formation and osteoclast-mediated resorption. In osteoporosis, this balance is disrupted, causing low bone density, increased fragility, higher fracture risk, and pathological bone defects. Current treatments focus on inhibiting resorption (e.g., bisphosphonates, RANKL inhibitors) or promoting formation (e.g., PTH analogues). However, long-term anti-resorptive use risks adverse effects like jaw osteonecrosis or atypical fractures, and drug therapy alone poorly addresses bone defects. Consequently, research increasingly explores natural products (e.g., traditional medicine compounds) and biomaterials for bone regeneration, aiming for safer, more effective therapies. This experiment develops a biomimetic mineralized hydrogel for bone defect implantation. Using carboxymethyl chitosan (CMCS) as the matrix, it cross-links alendronic acid (ALN) with the natural product genipin (GNP), aiming to promote bone regeneration in osteoporotic conditions. This compound is designated ICT@CMCS/ALN.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Nitong Bu,Runzhi Sun,Liying Huang,Huanglong Lin,Jie Pang,Lin Wang,Ruojun Mu
In this work, chitosan (CS) emulsion films were prepared with grapefruit essential oil (GEO) Pickering emulsions (OGEOs) stabilized by amphiphilic octenyl succinic anhydride (OSA) konjac glucomannan (OSA-KGM) network. The droplet size of emulsion was regulated by altering oil content in OGEOs (10 %, 20 %, 30 % and 40 %, w/w). The structural and physicochemical properties of CS films with tunable emulsion droplets (OGEOs) were investigated. The droplet size of OGEOs increased with the increasing content of GEO. FT-IR revealed that the formation of CS-OGEOs films was attributed to hydrogen bonding. CS-OGEOs films with large droplets presented smoother surface, enhanced water resistance, UV-shielding property, mechanical properties, but increased water vapor permeability (WVP) compared with CS-OGEOs films with small droplets. In addition, CS-OGEOs films with large droplets also presented compact film structure, controlled release of GEO, high efficiency of DPPH free radical scavenging and antibacterial activity. To sum up, incorporation of emulsion droplets was a good strategy for improving the structural and physicochemical properties of CS films.
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MATERIALS & DESIGN
·
2020
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IF 6.289
Fenghua Tao,Yanxiang Cheng,Hai Tao,Lin Jin,Zhihui Wan,Fangfang Dai,Wei Xiang,Hongbing Deng
Periosteum provides nourishment to bones and facilitates osteogenesis. The structural and functional impairment of periosteum can cause bone regeneration difficulties. Artificial periosteum with suitable mechanical properties and chemical composition can effectively guide bone tissue regeneration. In this study, a polycaprolactone (PCL)/carboxymethyl chitosan (CMCS)/sodium alginate (SA) micron-fibrous bionic periosteum was prepared. The water-in-oil PCL-CMCS/SA emulsion was prepared by using Span 80 as the emulsifier, and the PCL/CMCS/SA composite micron-fibers were fabricated by emulsion electrospinning. The physicochemical properties of the PCL/CMCS/SA micron-fibers were investigated via SEM, FTIR and tensile test analyses. CCK8 assay, live/dead assay, cell adhesion, and qPCR tests were carried out to evaluate the osteoinductive capacity of the micron-fibers and their biocompatibility to MC3T3-E1 osteoblasts. The results showed that CMCS and SA were successfully immobilized in the micron-fibers. The PCL/CMCS/SA micron-fibers showed an average diameter of 2.381 ± 1.068 μm with excellent tensile strength. The PCL/CMCS/SA composite scaffold demonstrated no significant cytotoxicity and could facilitate osteoblast adhesion. In addition, it could upregulate the early expression of osteogenic genes ALP and Runx2. The PCL/CMCS/SA micron-fibers prepared by emulsion electrospinning showed good mechanical properties, excellent biocompatibility and osteoinductive capacity to osteoblasts, and could be used as potential transplantable scaffolds for repairing large-segment bone defects.
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Experimental and Therapeutic Medicine
·
2018
·Vol. 15
·期 4
·3265-3272
·
IF 1.41
Yuan‑Jun Xia,Hong Xia,Ling Chen,Qing‑Shui Ying,Xiang Yu,Li‑Hua Li,Jian‑Hua Wang,Ying Zhang
Bone morphogenetic protein‑2 (BMP‑2) serves an important role in the development of bone and cartilage. However, administration of BMP‑2 protein alone by intravenous delivery is not very effective. Sustained delivery of stabilized BMP‑2 by carriers has been proven necessary to improve the osteogenesis effect of BMP‑2. The present study constructed a novel drug delivery system using dextran sulfate (DS)‑chitosan (CS) microspheres and investigated the efficiency of the delivery system on recombinant human bone morphogenetic protein (rhBMP‑2). The microsphere morphology, optimal ratio of DS/CS/rhBMP‑2, and drug loading rate and entrapment efficiency of rhBMP‑2 CS nanoparticles were determined. L929 cells were used to evaluate the cytotoxicity and effect of DS/CS/rhBMP‑2 microspheres on cell proliferation. Differentiation study was conducted using bone marrow mesenchymal stem cells (BMSCs‑C57) cells treated with DS/CS/rhBMP‑2 microspheres or the control microspheres. The DS/CS/rhBMP‑2 microspheres delivery system was successfully established. Subsequent complexation of rhBMP‑2‑bound DS with polycations afforded well defined microspheres with a diameter of ~250 nm. High protein entrapment efficiency (85.6%) and loading ratio (47.245) µg/mg were achieved. Release of rhBMP‑2 from resultant microspheres persisted for over 20 days as determined by ELISA assay. The bioactivity of rhBMP‑2 encapsulated in the CS/DS microsphere was observed to be well preserved as evidenced by the alkaline phosphatase activity assay and calcium nodule formation of BMSCs‑C57 incubated with rhBMP‑2‑loaded microspheres. The results demonstrated that microspheres based on CS‑DS polyion complexes were a highly efficient vehicle for delivery of rhBMP‑2 protein. The present study may provide novel orientation for bone tissue engineering for repairing and regenerating bone defects.
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