查找使用阿拉丁产品的科研文献

Anisotropy-mediated stress regulation in Mn-substituted VOPO4 enables aqueous zinc batteries with long cycle life

Yao Zhenjie,Zhang Wenyao,Chen Xinyu,Cui Junjie,Zhu Boyuan,Tang Jiahao,Hou Yangrui,Chen Jiarong,Tang Li,Zeng Jianrong,Jia Hongbing,Antonietti Markus,Zhu Junwu

The structural integrity of layered VOPO 4 frameworks critically determines their electrochemical performance in aqueous zinc-ion batteries, yet the highly ordered stacking of VOPO 4 renders it susceptible to lattice stress accumulation and chemo-mechanical degradation during cycling, causing rapid capacity fading and a shortened battery lifespan. Herein, we show a strategy to alleviate these limitations through partial metal substitution in the VOPO 4 lattice, enabling the modulation of local coordination environments and mitigating lattice and cycling induced stress. As a proof-of-concept, theoretical calculations and experimental validation on Mn-substituted VOPO 4 reveal shortened M–O bond along the c-axis and the formation of Mn–O–P–O–V delocalized structure. These modifications introduce subtle lattice distortions and generate percolation channels that enable more facile, ordered Zn 2+ migration, facilitating uniform lattice stress distribution. Real-time monitoring of interfacial stress during cycling identifies that Mn incorporation induces electronic redistribution and lattice anisotropy, promoting balanced intra-layer (a/b-axis) stress accommodation. Therefore, Mn-substituted VOPO 4 exhibits higher specific capacity and cycling stability, with 91.0% capacity retention over 2000 cycles at 1 A g −1 . This intra-layer anisotropy engineering strategy offers a practical route for mitigating dissolution and strain effects during cycling, paving ways for the development of high-energy-density positive electrodes with cycling durability.

A Coordination-Induced In Situ Self‐Assembly Strategy for High Structurally Stable Carboxymethyl Chitosan@HKUST-1/Polyvinylidene Fluoride Electrospun Fibers

Li Qiaomei,Hong Wei,Wei Tianyu,Fan Yupeng,Luo Yuhui,Li Jinlou,Qiao Zhihua,Jia Xuemeng,Zhang Dong-En

Metal-organic frameworks (MOFs) have shown great promise for water treatment owing to their tunable porous structures and abundant active sites. However, the practical application of conventional MOFs, such as HKUST-1, is still limited by insufficient structural stability in aqueous environments and poor recoverability in powder form. Herein, a coordination-induced in situ self-assembly strategy was developed to fabricate structurally stable carboxymethyl chitosan (CMCS)@HKUST-1/polyvinylidene fluoride (PVDF) electrospun fibers. In detail, Cu 2+ was first incorporated into PVDF matrix by electrospinning to obtain Cu 2+ /PVDF fibers, which were subsequently immersed in a mixed ligand solution containing 1,3,5-benzenetricarboxylic acid (H 3 BTC) and CMCS, enabling the in situ growth of CMCS-modified HKUST-1 on the fiber matrix. CMCS participated in the coordination-induced assembly process mainly through -COOH/-COO⁻, thereby strengthening the Cu-O coordination environment and improving the resistance of HKUST-1 to water-induced structural degradation. Meanwhile, the flexible CMCS chains and their hydrophilic functional groups enhanced the interfacial interaction, wettability, and accessibility of adsorption sites within the composite fibrous network. The practical potential of CMCS@HKUST-1/PVDF electrospun fibers was evaluated through the treatment of simulated dyeing wastewater, in which it exhibited a high Congo red adsorption capacity of 173.08 mg/g and maintained robust structural stability under elevated temperature treatment at 70 ℃, as well as in aqueous environments over a pH range of 6–10. This work provides a facile and effective strategy for constructing recoverable, structurally stable, and high-performance MOF-based fibrous adsorbents for water treatment applications.

Direct electrosynthesis of acetamide from CO2 and nitrate via an atomically engineered dual-site catalyst

Xia Shuai,Tan Hao,Zhang Jianfang,Han Miao,Xu Chang,Yu Cuiping,Chen Kui,Zhang Yong,Wu Yucheng,Wang Yan

The electrocatalytic synthesis of amides from abundant small molecules offers a sustainable route for green chemical production, yet faces fundamental challenges due to kinetic competition between C–C and C–N bond formation. Here we show an atomically engineered dual-site catalyst featuring nickel single atoms adjacent to copper nanoclusters (Ni-SA/Cu-NCs) on a nitrogen-doped carbon matrix for efficient CO 2 and NO 3 − co-reduction to acetamide. This architecture enables complementary functions, with Ni sites selectively converting CO 2 to CO and neighboring Cu nanoclusters promoting C–C coupling to form the *CCO intermediate while concurrently reducing NO 3 − to form the *NH 2 intermediate. The resulting synergy facilitates rapid intermediate transfer and C–N coupling, delivering an acetamide yield rate of 257.3 mmol h −1 g cat . −1 at an industrial current density of 215.7 mA cm −2 , with stable operation over 160 h. In situ spectroscopic studies and theoretical calculations suggest that strong Ni–Cu electronic coupling promotes reactant adsorption and reduces the activation barriers for critical steps, including *CO dimerization and *CCO–*NH 2 coupling. This work provides an atomic-level design strategy for multi-site catalysts to steer complex electrocatalytic reactions toward value-added products.

EEG and fNIRS evidence for brain responses to three tannins: implications for astringency surface sub-quality in red wine

Shi Baohui,A Liya,Wei Ziyu,Blank Imre,Song Linna,Zhang Bo,Liu Yuan,Ma Wen

The astringency surface sub-quality is pivotal to red wine perception, yet its neural bases remain unclear. The approaches of non-invasive neurophysiological techniques, sensory analysis, liquid chromatography-mass spectrometry (LC-MS) analysis, and molecular docking were applied to examine the astringency surface sub-quality responses to three tannins—tannic acid, grape tannin and wine tannin—standardized to equivalent astringency intensity. The results showed that the three tannins at iso-intensity evoked distinct brain activity and sensory responses (wine tannin > tannic acid > grape tannin). The electroencephalography (EEG) results showed that frontal activation was significantly higher than in other regions, with differential delta-band power, while functional near-infrared spectroscopy (fNIRS) revealed corresponding changes in oxygenated hemoglobin across different frontal cortical regions, most prominently at channel 6 in the ventromedial prefrontal cortex. LC-MS analysis of tannins indicated that higher levels of tannin oligomers, polymeric pigments and free anthocyanins were associated with finer astringency sub-quality and stronger neurophysiological responses. By establishing a clear link between chemical composition and neural activation, this investigation demonstrates the efficacy of using neurophysiological measures as a robust, objective tool to complement and refine traditional sensory evaluation of wine.

Mannan oligosaccharide-S. typhimurium interaction mediated fluorescent-recovery of carbon dots/molybdenum disulfide probe for rapid detection

Ma Guozhi,Wang Xuan,Cai Jihai,Wang Xiaoying

The rapid detection of Salmonella is an essential precautionary measure to ensure food safety and prevent foodborne illness. In this study, based on the specific binding between mannan oligosaccharides (MOS) and type I fimbriae of Salmonella typhimurium ( S. typhimurium ), an efficient sensing strategy was developed. Obtained by enzymatic hydrolysis of konjac glucomannan, MOS were grafted to mannan-oligosaccharide modified carbon dots (g-CDs-MOS). It is worth noting that the introduction of MOS reduced the dissociation constant (K D ) between fabricated g-CDs-MOS and Con A from 25.4 µM (g-CDs and ConA) to 6.22 µM. This enhanced binding affinity stem from elevated density of MOS on surface of g-CDs. Furthermore, to construct recovery-type fluorescent probe, g-CDs-MOS was bonded onto two-dimensional quencher such as 3-aminobenzeneboronic acid-functionalized graphene oxide (GO-PBA), MnO 2 or MoS 2 . The g-CDs-MOS/MoS 2 exhibited the best sensitivity due to the high electron mobility of MoS 2 . Its fluorescent intensity was logarithmically responsive to concentration of S. typhimurium within the range of 10 2 -10 8 CFU·mL − 1 , and the corresponding limit of detection was as low as 11.79 CFU·mL − 1 . This fluorescent sensor shows promise for the detection of S. typhimurium in food safety. Graphical

PD-L1 vNAR promotes hair growth by modulating hair shaft elongation through activating the leptin-STAT3 signaling

Yang Minghan,Chen Jinlin,Huang Yi,Zheng Bowen,Cheng Yunying,Chen Jianming,Chen Ming

Programmed death ligand 1 (PD-L1) antibodies have achieved significant success in cancer immunotherapy. However, their roles beyond tumor treatment are not fully understood. In this study, we developed a shark-derived variable new antigen receptor targeting PD-L1, named 3P17, and investigated its role in promoting hair growth in C57BL/6J mice. The intravenous administration of 3P17 increased the proliferation of hair matrix cells, promoted hair shaft elongation, and accelerated hair follicle entry into anagen phage. Moreover, transcriptomic analysis showed that 3P17 increased leptin expression in mouse dorsal skin. Intravenous leptin phenocopied the hair shaft-elongating effect of 3P17. In contrast, the leptin receptor antagonist Allo-aca abrogated 3P17-induced hair shaft elongation. PD-L1, leptin, and phosphorylated STAT3 were co-expressed in hair matrix cells, and both 3P17 and leptin increased STAT3 phosphorylation in mouse dorsal skin. 3P17 also increased the expression of the proliferation-associated genes cyclin D1 and c-Myc in hair follicles. These results collectively indicated that the involvement of leptin-STAT3 signaling in the hair-growth-promoting effect of 3P17. To enhance drug delivery efficiency and patient compliance, we further evaluated the transdermal delivery of leptin using hollow sponge Haliclona sp. spicules (oSHS). oSHS-mediated delivery increased the proportion of leptin penetrating the skin to 43.69 ± 3.66% of the applied dose, representing a 3.28 ± 0.26-fold increase compared with leptin alone. Consistent with this enhanced uptake, oSHS increased leptin deposition into the viable epidermis and dermis, compared with microneedling and SHS, and promoted hair shaft elongation and hair growth in a mouse model of androgenetic alopecia. We propose oSHS-mediated transdermal delivery of leptin as a promising strategy to stimulate hair growth. Graphical abstract

β-hydroxybutyrate regulates microglia M1/M2 polarization and phagocytosis through the JAK1/STAT1 pathway to reduce neuroinflammation and exert anti-epileptic effects

Yang Hui,Wang Huifen

Isorhamnetin inhibits bladder cancer progression by mediating glycolysis through targeting the SRC/PI3K/Akt axis

Haiya Feng,Qianqian Li,Yangjun Lu,Hui Wang

Objective This study aimed to investigate the anti-tumor effects of the natural flavonoid Isorhamnetin (ISO) on BC and elucidate its underlying molecular mechanisms. Methods We integrated network pharmacology and bioinformatic analysis to predict the core targets and pathways between BC and ISO. In vitro functional assays were performed using the human BC cell line 5637. Cell viability, proliferation, apoptosis, migration, invasion, and adhesion were assessed via CCK-8, colony formation, flow cytometry, Transwell, and adhesion assays. Glycolytic function was evaluated by measuring extracellular acidification rate (ECAR), oxygen consumption rate (OCR), glucose uptake, and lactate production. Molecular docking and cellular thermal shift assays (CETSA) were used to validate ISO-SRC binding. Western blot and RT-qPCR analyses were conducted to examine protein and mRNA expression of key signaling and glycolytic components. For the rescue experiments, SRC was overexpressed along with treatment using the PI3K inhibitor LY294002. Results ISO significantly inhibited BC cell proliferation, migration, invasion, adhesion, and glycolysis while inducing G0/G1 phase arrest and apoptosis. Network pharmacology and molecular docking identified SRC as a direct target of ISO, with CETSA confirming their binding. ISO treatment downregulated phosphorylation of SRC and its downstream effectors (PI3K, Akt, mTOR), reduced the expression of glycolytic enzymes (HK2, GLUT1, PFKFB3), and decreased ECAR, OCR, glucose uptake, and lactate production. SRC overexpression reversed the anti-tumor and anti-glycolytic effects of ISO. Furthermore, administration of LY294002 on this basis counteracted the effects induced by SRC overexpression, confirming the critical role of the SRC/PI3K/Akt axis. Conclusion ISO inhibits the malignant progression of BC by directly targeting SRC to suppress glycolysis mediated by the PI3K/Akt signaling pathway. These findings provide a mechanistic basis for considering ISO as a potential metabolic-targeting agent for BC, warranting further preclinical evaluation.

Lethal effect of trehalose-6-Phosphate phosphatase inhibitor N-(phenylthio) phthalimide on Trichinella spiralis

Hou Jiaming,Ruan Rulin,Zhang Ting,Yang Yongqing,Xue Han,Song Mingxin,Han Caixia

In eukaryotes, with the exception of mammals, trehalose fulfills multiple biological functions, including energy provision and stress tolerance. Trehalose-6-phosphate phosphatase (TPP) is a key enzyme in the trehalose biosynthetic pathway, rather than a trehalose synthase per se. Loss of TPP function or knockdown of its expression impairs trehalose synthesis, which in turn leads to developmental abnormalities or lethality in nematodes. These observations suggest that TPP represents a potential target for the development of anti-parasitic therapeutics. This study focused on the cloning, expression, and characterization of Trichinella spiralis TPP (TsTPP). A recombinant plasmid was constructed to express and purify the recombinant TsTPP protein (rTsTPP), whose immunogenicity was confirmed using positive serum from Trichinella spiralis ( T. spiralis )-infected mouse. The expression of TsTPP at different developmental stages of T. spiralis was determined by immunofluorescence assay. The specific TPP inhibitor N-(phenylthio) phthalimide (NPP) was employed to evaluate its lethal effect on T. spiralis muscle larvae (ML) in vitro. Furthermore, molecular methods such as qPCR and Western blot were used to detect the transcription and protein expression levels of TsTPP upon NPP treatment, aiming to identify new drug targets against T. spiralis . We successfully constructed the recombinant plasmid and obtained rTsTPP. Positive serum assays demonstrated the immunogenicity of rTsTPP, and we used indirect immunofluorescence assay (IFA) to determine its localization across different developmental stages of T. spiralis . Distinct distribution patterns were observed: TsTPP was diffusely distributed throughout the body of newborn larvae (NBL), accumulated in the intestinal glands, midgut ampulla and genital primordia of muscle larvae (ML), and localized to the mid and posterior intestine of adult worms (AW). To verify the lethal effect of NPP in vitro, we treated ML with NPP at concentrations of 12.5, 25, 50, 100, and 200 µg/mL for 24, 36, and 48 h, and evaluated its lethal effect by statistical analysis of worm morphology. The experimental results showed that the mortality rate of ML gradually increased with increasing NPP concentration and culture time. When the concentration was 200 µg/mL, the survival rate of ML was less than 50%. Furthermore, when treated with NPP at concentrations of 12.5, 25, 50, 100, and 200 µg/mL for 24 h, the transcription levels of the TsTPP gene decreased by 19%, 23%, 30%, 38% (all p  < 0.05), and 57% ( p  < 0.01), respectively; after 36 h of treatment, the transcription levels of TsTPP gene were reduced by 18% ( p  < 0.05), 23%, 35%, 39%, and 60% (all p  < 0.01); after 48 h of treatment, the transcription levels of TsTPP gene decreased by 19%, 23%, 42%, 42%, and 65% (all p  < 0.01), respectively. N-(phenylthio) phthalimide (NPP) exhibits significant lethal activity against T. spiralis by inhibiting the trehalose synthesis pathway, providing basic data for future NPP as a new clinical drug target.

Application of carbon-based nanocomposite sensors in wastewater heavy metal detection

Zhou Shiyu,Liu Jiahui,Chen Zhen,Zhou Jia

The accumulation of lead ions in industrial wastewater poses a long-term threat to ecosystems and health. Traditional detection techniques often fail to maintain sensitivity, selectivity, and signal stability in complex matrices. This paper builds a multilevel conductive composite structure based on covalent organic framework, platinum nanoparticles, and carboxylated multiwalled carbon nanotubes, and also builds an enhanced luminescent interface with gold nanoparticles and boron nitride quantum dots to improve electronic transmission and target recognition. The experimental results show a good linear relationship in the range of 0.005 to 50 ng/mL, and the luminescent signal reaches about 14,000 a.u. The interfacial resistance decreases to 0.78 kΩ, and the system gives a clear charge response after exposure to lead ions. The spiked recovery rate is up to 99.32% in wastewater samples and reaches a signal retention of 93.13% after 30 days. All the results indicate that the composite structure supplies high sensitivity, good selectivity, and strong stability in the detection of lead ions, offering an effective technical approach toward monitoring heavy metals in such complex wastewater.

Concave-pore defective BiOCl applied to photodegradation of Rhodamine B: mechanism, degradation pathway and toxicity analysis

Guo Jing,Zhao Xiaomei,Wang Qi,Wu Rui,Zhu Lexuan,Zhang Xin,Zhang Xinke,Gu Yuke

Morphological engineering is utilized to modify the microstructure and electronic structure of bismuth oxychloride; this led to a remarkable enhancement in photocatalytic performance. In this research, H-BOC nanosheets with concave-pore defects were synthesized via a glycol-mediated solvothermal etching method. Photocatalytic activity was examined using Rhodamine B (RhB, 20 mg·L⁻ 1 ) as the target contaminant under visible-light exposure. H-BOC displayed superior photocatalytic performance within 4 min, achieving a degradation rate of 99% and a reaction rate constant of 0.9643 min –1 , significantly higher than those of T-BOC (53% and 0.1556 min –1 ). The results indicate that the incorporation of concave-pore defects not only improves visible-light absorption and reduces hole migration distance but also enhances charge separation and effectively suppresses the recombination of photogenerated electron–hole pairs. This acceleration in reaction kinetics was further confirmed by trapping experiments and EPR analyses, which reveal that superoxide (•O 2 ⁻) and hydroxyl radicals (•OH) were the dominant reactive species. Furthermore, high-performance liquid chromatography–mass spectrometry (HPLC–MS) and the toxicity estimation software tool (T.E.S.T) were employed to investigate the degradation mechanisms, pathways, and the toxicity of RhB and its intermediates. This study presents a novel approach for designing and developing high-performance photocatalysts.

Parabacteroides distasonis ameliorates methylphenidate hydrochloride-induced colonic inflammatory responses and associated gastrointestinal distress

Yao Jinwen,Gao Xia,Zhu Lin,Wang Linlin,Chen Qian,Liang Weijuan,Feng Huaili,You Ting,Wei Changli,Liu Yuqi,Zhang Hongyang,Chen Chengzhi,Chen Li

Background Attention-deficit/hyperactivity disorder (ADHD) is a common neurodevelopmental disorder in childhood, with methylphenidate hydrochloride (MPH) as its first-line pharmacotherapy. Though effective, MPH is associated with underrecognized gastrointestinal adverse events warranting further investigation. Recently, the gut microbiota has attracted attention for treating gastrointestinal distress, but research on its role in alleviating MPH-induced gastrointestinal reactions remains scarce. Methods Herein, Wistar-Kyoto rats (WKY) served as controls alongside spontaneously hypertensive rats (SHR) to explore MPH-induced gastrointestinal adverse drug reactions. The gastrointestinal phenotypes, colonic histopathological characteristics, and inflammatory factor levels were evaluated, and the gut microbiota profiles were analyzed via 16S rRNA gene sequencing. The protective effect of Parabacteroides distasonis ( P. distasonis ) was further verified by supplementing the treated rats with this strain. Results We demonstrated MPH induced diarrhea and colonic inflammatory responses in rats. 16S rRNA gene amplicon sequencing revealed that MPH-treated rats had significantly decreased gut microbiota health index, increased dysbiosis index, and reduced P. distasonis abundance, suggesting MPH-induced gastrointestinal reactions may correlate closely with reduced P. distasonis . Further experiments confirmed that P. distasonis supplementation effectively alleviated diarrhea and colonic inflammatory responses in MPH-treated rats. Conclusions Collectively, this is the first study verifying that P. distasonis supplementation mitigates MPH-induced gastrointestinal adverse reactions, providing a novel intervention strategy to improve tolerance of ADHD pharmacotherapy.