ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2020
·
IF 4.872
Biao Chen,Qing Chai,Shengmin Xu,Qian Li,Tao Wu,Shaopeng Chen,Lijun Wu
Silver nanoparticles (AgNPs) are widely used as antimicrobial agents and resulted in their accumulation in environment. The purpose of this study was to investigate the detailed molecular mechanisms underlying AgNP-induced lung cellular senescence which has been proposed as a pathogenic driver of chronic lung disease. Herein, we demonstrate that exposure to AgNPs elevates multiple senescence biomarkers in lung cells, with cell cycle arrest in the G2/M phase, and potently activates genes of the senescence-associated secretory phenotype (SASP) in human fetal lung fibroblast cell line MRC5. Fluorescence-based assay also reveals that apoptosis induced by AgNPs is associated with senescence. Furthermore, we show that AgNPs cause premature senescence through an increase in transcription factor nuclear factor kappa B (NF- κ B), cyclooxygenase-2 (COX2) expression and over-production of prostaglandin E2 (PGE2) in lung cells. Inhibition of COX2 reduces AgNPs-induced senescence to a normal level. Moreover, AgNPs also induce upregulation of COX2 and accelerate lung cellular senescence in vivo and cause mild fibrosis in the lung tissue of mice. Taken together, our studies support a critical role of AgNPs in the induction of lung cellular senescence via the upregulation of the COX2/PGE2 intracrine pathway, and suggest the adverse effects to the human respiratory system.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Qiqi Yang,Yuan Yao,Junping Wang,Mengdi Pang,Jinyan Wu,Fusheng Yin,Jun He,Yanyan Wang,Weidong Chen
Non-small cell lung cancer (NSCLC) is characterized by a complex pathogenesis and high mortality. Targeted drug therapy represents a precise method of treatment. However, Improving sensitivity to targeted agents is currently a major challenge in clinical tumor therapy. Neogambogic acid (NGA), a bioactive compound isolated from the Traditional Chinese Medicine (TCM) Gamboge, has shown potential in NSCLC treatment. Nonetheless, the mechanism by which NGA enhances the sensitivity of NSCLC to the targeted drug Almonertinib remains unclear. This study aimed to elucidate the mechanisms underlying the ability of NGA to enhance the sensitivity of NSCLC to Almonertinib, revealing the synergistic effect exerted by the combination therapy. We established a subcutaneous grafted tumor model of mouse lung cancer by injecting Lewis cells, and evaluated the therapeutic efficacy in vivo . The study demonstrated that NGA combined with Almonertinib effectively suppressed NSCLC tumor growth by promoting cancer cell apoptosis and reducing serum tumor marker levels. In vitro experiments revealed that this combination synergistically inhibited lung cancer cell proliferation and induced apoptosis. Mechanistically, NGA demonstrated the ability to bind to Pregnane X receptor (PXR), regulating the expression of drug-metabolizing enzymes and transporters. We further validated this effect using PXR agonists and inhibitors in A549 cells. Additionally, the combination of NGA with Almonertinib significantly inhibited aberrant EGFR activation and downstream PI3K/AKT signaling, leading to enhanced apoptosis. This study demonstrated that the combination of NGA and Almonertinib inhibited the expression of CYP3A4, P-gp, and BCRP via PXR modulation. Meanwhile, it inhibited the EGFR/PI3K/AKT pathway and enhanced the sensitivity of NSCLC to Almonertinib, exerting synergistic anti-tumor effects. Our findings suggest a promising strategy for integrating active TCM components with targeted therapy in NSCLC management.
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INORGANIC CHEMISTRY
·
2025
·
IF 4.7
Fei Ma,Chao Wang,Yutong An,Yajie Liu,Xiaorong Xin,Kaicai Fan,Lei Wang,Guanjun Chen,Zhiqiang Hu,Tianrong Zhan
Cheap and advanced electrocatalysts for the oxygen evolution reaction (OER) are vitally significant but challenging for seawater electrolysis. Herein, a glow discharge N2 plasma is used to etch the [Fe(CN)6]4– group of the NiCoFe Prussian blue analogue (PBA) framework by highly reactive nitrogen radicals (the catalyst named N2-NiCoFe-PBA). Abundant CN vacancies (VCN) generated by this etching effectively suppress the Fe loss from N2-NiCoFe-PBA, while the lack of VCN results in the Fe leaching from the NiCoFe-PBA precursor during the anodic process. Consequently, N2-NiCoFe-PBA and NiCoFe-PBA are transformed into real active phases of NiCoFeOOH and NiCoOOH during the OER process, respectively. Theoretical calculations display that NiCoFeOOH has superior adsorption capacity toward OH– over Cl– to NiCoOOH, which is beneficial to excellent OER activity and anticorrosion performance in alkaline seawaters. In alkaline simulated and natural seawater, N2-NiCoFe-PBA exhibits much better OER performance with smaller η100 (293 and 323 mV) than NiCoFe-PBA (405 and 434 mV). N2-NiCoFe-PBA can run stably over 110 h in an alkaline high-salty electrolyte (1 M KOH + 2.0 M NaCl) at 250 mA cm–2. This study provides a modification method without destroying the structure to develop efficient and stable OER catalysts for seawater electrolysis.
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高影响因子
FUEL
·
2025
·
IF 7.5
Jie Gao,Hangjia Zhang,Jingyu Ran,Chuan Ma,Xin Huang,Yunlin Shao,Huayu Qiu,Zhiliang Ou,Lei Tang
Dry reforming of methane (DRM) is an efficient way to convert the two main greenhouse gases (CH 4 and CO 2 ) into syngas. This study reported an efficient CaCr 2 O 4 -supported Ni-Co bimetallic catalyst for its DRM activity and anti-coking performance through experiments, characterization, and DFT calculations, with an emphasis on elucidating the role of Ni-Co bimetals in CaCr 2 O 4 catalysts. The results demonstrated that the optimal 13Ni2Co/CaCr 2 O 4 catalyst achieved 94.8 % CH 4 and 96.8 % CO 2 conversions, under conditions of 800 °C and 27,000 mL·h −1 ·g −1 . Characterization results indicated that the alloying effect induced by introducing 2 wt% Co promoted the uniform dispersion of active metals, improved the reducibility and surface basicity of the catalyst, which collectively contributed to its superior catalytic performance. The catalyst also exhibited high thermal stability and excellent coke resistance at 800 °C, with a surface carbon deposition amount of only 0.93 % after 10 h continuous operation. This was attributed to the weak adsorption of surface carbon by Co, thereby reducing the carbon deposition degree. Moreover, the abundant strong basic sites on the surface significantly enhance CO 2 adsorption, while the surface-active adsorbed oxygen further facilitates the elimination of carbon deposits. This research provides valuable insights for the design and development of alloy catalysts in catalytic reaction.
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CATALYSIS LETTERS
·
2025
·Vol. 156
·期 1
·27
·
IF 2.4
Tian Hongkun,Hu Yuqi,Li Hao
Diisobutyl ketone (DIBK), a high-value chemical with growing market demand, is currently produced as a by-product in methyl isobutyl ketone (MIBK) synthesis, leading to high costs and limited output. This study investigates the one-step synthesis of DIBK from acetone condensation and hydroconversion using Pd/Beta zeolite catalysts. A series of catalysts were prepared via impregnation and systematically characterized by XRD, BET, NH₃-TPD, Py-IR, and XPS. The effects of zeolite type, SiO₂/Al₂O₃ ratio, Pd loading, and reaction conditions on catalytic performance were thoroughly examined. Results demonstrate that Pd/Beta-25, possessing the highest acid site density, optimal Brønsted-to-Lewis acid ratio, and superior Pd dispersion, achieved the best performance: 98.4% acetone conversion and 64% DIBK selectivity under optimal conditions (210 °C, 3 MPa H₂, 9 h). The synergy between abundant acid sites (facilitating condensation) and well-dispersed Pd nanoparticles (enabling hydrogenation) is crucial for promoting the consecutive reaction pathway to DIBK. This work provides fundamental insights and an efficient catalytic system for direct DIBK production, highlighting the advantage of zeolite-based catalysts over conventional Pd/resin systems. Graphical
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Advanced Science
·
2025
·
IF 14.1
Ningxi Wu,Anwen Yin,Xiang Yu,Mingxin Wang,Jiahui Zhang,Kerong Liu,Yutong Hou,Minkai Cao,Yiting Zhang,Xiaoxiao Zhang,Yao Gao,Xirong Guo,Le Zhang,Yun Li
Exclusive breastfeeding reduces the risk of childhood obesity, potentially through metabolic programming of adipose tissue during lactation. However, the underlying mechanisms remain unclear. Using a mouse model, it is shown that mixed formula feeding disrupts brown adipose tissue (BAT) morphology, mitochondrial integrity, and thermogenic capacity, resulting in greater fat accumulation and glucose intolerance after weaning under a high-fat diet. By contrast, BAT from exclusively breastfed mice preserved enhanced thermogenic function for up to 12 weeks after transplantation into recipient mice. Transcriptomic analysis revealed that AMPK activation is sustained in BAT from exclusively breastfed mice but markedly diminished in mixed-fed counterparts. Pharmacological inhibition of AMPK abolished the long-term metabolic benefits conferred by exclusive breastfeeding. Mechanistically, breast milk–derived extracellular vesicles enriched in miR-125a-5p enhanced AMPK signaling by targeting HIF1AN. AMPK-induced α-ketoglutarate (αKG) production proved essential for BAT development and thermogenesis, and αKG supplementation rescued impaired BAT function in mixed-fed mice. In conclusion, exclusive breastfeeding imprints a thermogenic memory in BAT via the HIF1AN/AMPK/αKG signaling axis, thereby conferring long-term metabolic protection to offspring.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jiacheng Wang,Yuhang Zhang,Yingbin Xu,Hongyu Jin,Can Yang,Hongxing Liu,Yongbo Bao
The blood clam ( Anadara granosa ) exhibits innovative red blood compared to other molluscs, with erythrocytes constituting 79 % to 89 % of the total hemocyte count. Previously, we identified that GATA-binding protein 1 (designated as Ag GATA1) was significantly up-regulated in the transcriptomes of blood clam populations with high hemocyte counts. However, the function of Ag GATA1 in erythropoiesis remains unknown. In this study, we conducted GATA1 knockdown experiments in A. granosa , which resulted in a significant reduction of both erythrocytes and other cells during the S phase, as well as significantly decreased proliferative activity in the gill tissue. The cell proliferation assay demonstrated that overexpression of Ag GATA1 increased the DNA replication activity of K562 cells. Additionally, o-dianisidine staining revealed defects in erythropoiesis within the gill tissue following Ag GATA1 knockdown, while protein analyses confirmed a significant reduction in Ag GATA1 expression. Furthermore, decreased cytoskeletal fluorescence and morphological changes in erythrocytes suggest that Ag GATA1 influenced cytoskeletal protein expression, leading to changes in erythrocyte morphology. This study provides new insights into the role of Ag GATA1 in hemocyte formation and highlights its significance in understanding erythropoiesis within invertebrate developmental tissues.
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APPLIED SURFACE SCIENCE
·
2016
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IF 3.15
Yufen Wei,Zhanqiang Fang,Liuchun Zheng,Eric Pokeung Tsang
Eichhornia crassipes (water hyacinth), a species of invasive weeds has caused serious ecological damage due to its extraordinary fertility and growth rate. However, it has not yet been exploited for use as a resource. This paper reported the synthesis and characterization of amorphous iron nanoparticles (Ec-Fe-NPs) from Fe(III) salts in aqueous extracts of Eichhornia crassipes . The nanoparticles were characterized by SEM, EDS, TEM, XPS, FTIR, DLS and the zeta potential methods. The characterization results confirmed the successful synthesis of amorphous iron nanoparticles with diameters of 20–80 nm. Moreover, the nanoparticles were mainly composed of zero valent iron nanoparticles which were coated with various organic matters in the extracts as a capping or stabilizing agents. Batch experiments showed that 89.9% of Cr(VI) was removed by the Ec-Fe-NPs much higher than by the extracts alone (20.4%) and Fe 3 O 4 nanoparticles (47.3%). Based on the kinetics study and the XPS analysis, a removal mechanism dominated by adsorption and reduction with subsequently co-precipitation was proposed.
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高影响因子
NEURON
·
2013
·
IF 15.766
Alejandro Javier Pernía-Andrade,Peter Jonas
Theta-gamma network oscillations are thought to represent key reference signals for information processing in neuronal ensembles, but the underlying synaptic mechanisms remain unclear. To address this question, we performed whole-cell (WC) patch-clamp recordings from mature hippocampal granule cells (GCs) in vivo in the dentate gyrus of anesthetized and awake rats. GCs in vivo fired action potentials at low frequency, consistent with sparse coding in the dentate gyrus. GCs were exposed to barrages of fast AMPAR-mediated excitatory postsynaptic currents (EPSCs), primarily relayed from the entorhinal cortex, and inhibitory postsynaptic currents (IPSCs), presumably generated by local interneurons. EPSCs exhibited coherence with the field potential predominantly in the theta frequency band, whereas IPSCs showed coherence primarily in the gamma range. Action potentials in GCs were phase locked to network oscillations. Thus, theta-gamma-modulated synaptic currents may provide a framework for sparse temporal coding of information in the dentate gyrus.
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高影响因子
WATER SCIENCE AND TECHNOLOGY
·
2017
·Vol. 75
·期 6
·1466-1473
·
IF 1.197
Li Shifeng,Qi Fang,Xiao Min,Fan Hongtao,Shen Yanming,Du Kaige,Zhang Zhigang,Li Wenxiu
Mg-Al layered double hydroxides (LDHs) adsorbent was synthesized in situ on γ -Al 2 O 3 for the removal of Cr(VI) from aqueous solution. The material was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, Raman spectroscopy, scanning electronic microscopy and thermogravimetry and differential thermal analysis. Compared to the LDHs powder, the calcined LDHs sorbent prepared in situ on γ -Al 2 O 3 had higher specific surface area and was easy to recover and reuse. The adsorptive capacity for removing Cr(VI) from aqueous solution was resulting from the memory effect of LDHs based on the XRD results. Both the pseudo-second-order kinetic model and the Langmuir model fit the experimental data well. Furthermore, the adsorbent exhibits excellent sorption–regeneration performances.
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OPTICAL MATERIALS
·
2021
·
IF 3.08
Xiang Meng,Jiajun Ma,Kang Xie,Liang Hong,Junxi Zhang,Zhijia Hu
We have demonstrated a low threshold and tunable flexible random laser (RL) in dye-doped poly ethylene glycol diacrylate (PEG-DA) hydrogel, which was prepared by crosslinking polymerization using PEG-DA monomer and photoinitiator. The random lasering is attributed to the light scattering from the porous structure of hydrogels, and the wavelength of random lasing can be tuned by ~3.2 nm in the temperature range from 30 °C to 70 °C. Under the continuous increasing and decreasing temperature cycle, the random lasing tunable phenomenon can still be observed, which proves that this flexible material can be used as a new temperature sensor . In addition, we also explored the application of flexible laser in phototherapy. Considering the good elasticity and transparency of hydrogels, this thermo-responsive property will be of great significance to the biomedical applications of flexible material.
研究领域:
生物医学工程
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光学工程
·
高分子材料科学
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光子学
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生物传感器
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MATERIALS CHEMISTRY AND PHYSICS
·
2026
·
IF 5.2
Wei Liao,Sheng Li,Yaru Liu
The influence of graphene on asphalt performance and its underlying multiscale modification mechanisms remains insufficiently understood. This study combined morphological, thermal, and rheological tests with molecular dynamics simulations and quantum mechanical (QM) calculations to investigate graphene modified asphalt. The results show that graphene was relatively uniformly dispersed at 1.0–1.5 wt%, whereas noticeable aggregation and structural heterogeneity appeared above 2.0–2.5 wt%. At 3.0 wt%, the glass-transition temperature shifted downward by 6.07 °C, while the heat-capacity change ( ΔCp ) decreased by 37.37 %, and the thermal conductivity (TC) increased from 0.161 to 0.272 W/(m·K). Graphene increased complex shear modulus ( G* ) and rutting factor ( G*/ sin δ ) and reduced the phase angle ( δ ), indicating enhanced stiffness and elasticity under small-strain loading. However, increased J nr and J nr-diff revealed greater residual deformation and stress sensitivity under repeated creep loading, particularly at higher dosages. Nonequilibrium molecular dynamics (NEMD) and reverse nonequilibrium molecular dynamics (RNEMD) simulations reproduced the experimental trends and further revealed that the thermal-conductivity enhancement was associated with strengthened interfacial energy transfer, restricted component diffusion, and reduced fractional free volume ( FFV ), rather than only the intrinsic conductivity of graphene. Density functional theory (DFT) calculations further clarified the electronic origin of graphene–asphalt affinity, showing that molecular planarity, π-conjugation, and heteroatomic groups controlled the selective adsorption of asphalt components on graphene through dominant van der Waals interactions and π–π interactions. These findings establish a cross-scale correspondence among experimental performance, collective molecular transport, and molecule-specific electronic interactions, thereby clarifying the dosage-dependent balance among thermal enhancement, structural reinforcement, and deformation stability.
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