Journal of Neuroinflammation
·
2025
·Vol. 22
·期 1
·1-19
·
IF 10.1
Chi Zhexi,Lu Bo,Liu Rongjun,Pan Chen,Meng Bo,Xing Xiuzhong,Yuan Hui,Wu Xuewei,Chen Yushan,Ren Yuxuan,Wu Wenwei,Miao Mengmeng,Chen Junping,Chen Xiaowei
Neuropathic pain, a debilitating nerve injury-induced condition, remains a significant clinical challenge. This study evaluates the effect of histone deacetylase 6 (HDAC6) inhibition in a spared nerve injury (SNI) mouse model. Systemic administration of the selective HDAC6 inhibitor ACY-1215 (20 mg/kg/day, 14 days), alleviated SNI-induced pain in mice of both sexes. ACY-1215 increased acetylated signal transducer and activator of transcription 3 (Ac-STAT3) and reduced phosphorylated STAT3 (p-STAT3) in the lumbar spinal cord of SNI mice. HDAC6 and p-STAT3 were expressed in spinal dorsal horn neurons, and SNI-enhanced HDAC6/STAT3 interaction was reversed by ACY-1215. Neuronal STAT3 overexpression induced pain hypersensitivity and elevated p-STAT3, tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β), effects suppressed by ACY-1215. Cytokine profiling identified CC-chemokine ligand 7 (CCL7) as a key downstream effector of the HDAC6/STAT3 axis, with ACY-1215 attenuating SNI-induced CCL7 upregulation. HDAC6 knockdown in neurons reduced p-STAT3, while HDAC6 or STAT3 knockdown diminished CCL7 production. These findings demonstrate that ACY-1215 mitigates neuropathic pain by modulating STAT3 acetylation/phosphorylation and suppressing HDAC6/STAT3-driven CCL7 and cytokine release. This study underscores the role of the HDAC6/STAT3/CCL7 signaling axis in neuropathic pain and highlights the therapeutic potential of HDAC6 inhibitors for pain management.
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Advanced Fiber Materials
·
2024
·Vol. 6
·期 4
·1188-1211
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IF 17.2
Yu Weikang,Guo Xu,Li Qingrui,Li Xuefeng,Wei Yingxin,Shao Changxuan,Zhang Licong,Wang Jiajun,Shan Anshan
Incorporating enzyme-resistant peptide sequences into self-assembled nanosystems is a promising strategy to enhance the stability and versatility of peptide-based antibacterial drugs, aiming to replace ineffective antibiotics. By combining newly designed enzymatic-resistant sequences with synthetically derived compounds bearing single, double, triple, or quadruple aromatic rings. A series of nanoscale antimicrobial self-assembled short peptides for the purpose of combating bacterial infections are generated. Nap* (Nap– D Nal–Nal–Dab–Dab–NH 2 , where Nap represents the 1-naphthylacetyl group) possesses the greatest clinical potential (GM SI = 23.96) among the peptides in this series. At high concentrations in an aqueous environment, Nap* spontaneously generates nanofibers to capture bacteria and prevent their evasion, exhibiting broad-spectrum antimicrobial effects and exceptional biocompatibility. In the presence of physiological salt ions and serum, the antimicrobial agent exhibits strong effectiveness and retains impressive resistance even when exposed to high levels of proteases (trypsin, chymotrypsin, pepsin). Nap* exhibits negligible in vivo toxicity and effectively alleviates systemic bacterial infections in mice. Mechanistically, Nap* initially captures bacteria and induces bacterial cell death primarily through membrane dissolution, achieved by multiple synergistic mechanisms. In summary, these advances have the potential to greatly expedite the clinical evolution of nanomaterials based on short peptides combined with naphthyl groups and foster the development of peptides integrated with self-assembled systems in this domain. Graphical Abstract
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Advanced Fiber Materials
·
2024
·Vol. 6
·期 2
·501-511
·
IF 17.2
Xie Maowen,Yao Guang,Gan Xingyi,Zhang Chunhong,Zhang Tianyao,Wang Qian,Li Xinqin,Zhou Chenzheng,Zhao Kangning,Gao Min,Pan Taisong,Lin Yuan
Flexible electrochemical biosensors enable the in-situ monitoring and quantification of human biochemical constituents in molecular scale, spearheading and thriving the field toward precision medicine. However, specific biorecognition elements for multiplexed biomarkers detection, temperature stability and versatility need to be improved for higher adaption. Here, we propose a bioactive sensor patch comprising a non-enzyme Co 3 O 4 /carbon fiber-based biorecognition element and a temperature calibration unit. The optimized serpentine configuration renders the sensor intimate and seamless attachment with skin, operating robustly even subjected to 40% tensile strain. The fiber-based sensor could selectively monitor dopamine and lactic acid contents based on cyclic voltammetry and amperometry, respectively. The bioanalytical results at room temperature indicate that the electrochemical biosensor has a wide detection range (0.001–1.100 mM for dopamine and 2–35 mM for lactic acid), excellent selectivity and reproducibility (maximum error 3.2% for dopamine and 5.6% for lactic acid). In addition, temperature calibration contour maps of these two biomarkers are established in an ambient temperature range from 25 to 45 ℃. The continuously collected data could be aggregated and wirelessly transmitted to portable devices using an electrochemical signal transducer and an acquisition module, promising personalized and preventative health care in various scenarios. Graphical Abstract This work proposes a bioactive fiber-based sensor patch comprising a non-enzyme Co 3 O 4 /carbon fiber-based biorecognition element and a temperature calibration unit. The sole fiber-based sensor could selectively monitor dopamine and lactic acid contents based on cyclic voltammetry and amperometry, exhibiting a wide detection range, excellent selectivity and reproducibility with temperature calibration in an ambient temperature range from 25 to 45 ℃, promising personalized and preventative health care in various scenarios
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CARDIOVASCULAR DRUGS AND THERAPY
·
2025
·
IF 3.1
Huang Jie,Hou Xingyuan,Zhou Ni,Su Nan,Wei Shanshan,Yang Yuanying,Sun Taoli,Li Guangdi,Li Wenqun,Zhang Bikui
Purpose Doxorubicin (Dox) is a classic anthracycline chemotherapy drug with cause cumulative and dose-dependent cardiotoxicity. This study aimed to investigate the potential role and molecular mechanism of phenylacetylglutamine (PAGln), a novel gut microbiota metabolite, in Dox-induced cardiotoxicity (DIC). Methods DIC models were established in vivo and in vitro, and a series of experiments were performed to verify the cardioprotective effect of PAGln. RNA sequencing (RNA-seq) was employed to explore the mechanism of PAGln in DIC. Subsequently, the differentially expressed genes (DEGs) were subjected to comprehensive analysis using diverse public databases, and RT-PCR was used to confirm the expression levels of the candidate genes. Finally, molecular docking techniques were used for validation. Results PAGln effectively prevented both in vivo and in vitro Dox-induced myocardial injury and cell apoptosis. RNA-seq results showed that 40 genes were up-regulated and 54 down-regulated in the Dox group compared to the Con group, displaying opposite changes in the Dox + PAGln group. Enrichment analysis highlighted several mechanisms by which PAGln alleviated Dox-induced cardiotoxicity, including the lipid metabolic process, calcium-mediated signaling, positive regulation of store-operated calcium channel activity, and hypertrophic cardiomyopathy. In vitro and in vivo experiments confirmed that PAGln treatment could reverse the changes in the expression levels of Klb, Ece2, Nmnat2, Casq1, Pak1, and Apob in Dox. Molecular docking results showed that these genes had good binding activity with PAGln. Conclusions PAGln shows potential in alleviating Dox-induced cardiotoxicity, with Ece2 identified as key regulatory molecules related to endothelial dysfunction.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
·
IF 15.7
Yu Bingcheng,Shi Jiangjian,Li Yiming,Tan Shan,Cui Yuqi,Meng Fanqi,Wu Huijue,Luo Yanhong,Li Dongmei,Meng Qingbo
Carbon-based perovskite solar cells exhibit a promising application prospect due to its cost effective and attractive hydrophobic nature and chemical inertness, but are still limited to unsatisfied device efficiency. Herein, we design a triple-layer full-carbon electrode for n-i-p typed perovskite solar cells, which is comprised of a modified macroporous carbon layer, a highly conductive graphite layer and a thin dense carbon layer, and each layer undertakes different contribution to improving the cell performance. Based on this full-carbon electrode, inorganic CsPbI 3 perovskite solar cells exhibit >19% certified efficiency which is the highest result among carbon-based CsPbI 3 devices. On one hand, carbon quantum dots decorated on the macro-porous carbon layer can realize better energy alignment of full-carbon electrode/spiro-OMeTAD/CsPbI 3 interface, on the other hand, highly conductive graphite layer is advantageous to carrier transporting. Typically, the top dense carbon layer exhibits significant thermal radiation ability, which can reduce the operational temperature of devices by about 10 °C, both from theoretical simulation and experimental testing. Thereby, packaged full-carbon electrode based CsPbI 3 cells exhibit much better photothermal stability at ~70°C accompanied by white light emitting diode illumination, which exhibit no efficiency degradation after 2000 h continuous operational tracking.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2025
·Vol. 36
·期 10
·1-18
·
IF 2.8
Huo Dongyu,Wang Qing,Zhu Fuliang,Wang Can,Dai Yile,Dai Jianfeng
Silicon-based anode materials have attracted significant interest due to their high theoretical specific capacity. However, their practical application is severely hindered by the substantial volumetric expansion during cycling. To overcome this limitation, a novel silicon–carbon nanofiber/carbonized Poria powder composite was synthesized via electrospinning followed by a simple heat treatment process. The carbonized Poria powder features a hierarchical porous structure, combining macropores and mesopores. The macropores effectively trap and constrain the silicon–carbon nanofibers, thereby enhancing the structural stability, while the mesopores increase the specific surface area, facilitating improved electrolyte penetration. The composite benefits from the conductive framework of nitrogen-doped carbon fibers and carbonized Poria powder, which collectively enhance its electrochemical performance. At a current density of 0.1 A g −1 , the composite exhibits an initial discharge capacity of 1579.7 mAh g −1 and a charge capacity of 1455.5 mAh g −1 . After 1000 cycles, the discharge capacity is maintained at 1277.2 mAh g −1 , corresponding to a capacity retention rate of 80.8%. Additionally, at a scan rate of 0.5 mV s −1 , the composite demonstrates a high pseudocapacitive contribution of 91.56%, which improves the lithium-ion diffusion rate, reduces cycling-induced stress and side reactions, and mitigates capacity degradation over extended cycling.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-14
·
IF 17.694
Xu Zhichen,Chen Dongjuan,Li Tao,Yan Jiayu,Zhu Jiang,He Ting,Hu Rui,Li Ying,Yang Yunhuang,Liu Maili
Fast, inexpensive, and multiplexed detection of multiple nucleic acids is of great importance to human health, yet it still represents a significant challenge. Herein, we propose a nucleic acid testing platform, named MiCaR, which couples a mi crofluidic device with CRISPR- C as12a a nd multiplex r ecombinase polymerase amplification. With only one fluorescence probe, MiCaR can simultaneously test up to 30 nucleic acid targets through microfluidic space coding. The detection limit achieves 0.26 attomole, and the multiplexed assay takes only 40 min. We demonstrate the utility of MiCaR by efficiently detecting the nine HPV subtypes targeted by the 9-valent HPV vaccine, showing a sensitivity of 97.8% and specificity of 98.1% in the testing of 100 patient samples at risk for HPV infection. Additionally, we also show the generalizability of our approach by successfully testing eight of the most clinically relevant respiratory viruses. We anticipate this effective, undecorated and versatile platform to be widely used in multiplexed nucleic acid detection.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-12
·
IF 17.694
Tan Peng,Wang Haifei,Xiao Furui,Lu Xi,Shang Wenhui,Deng Xiaobo,Song Huafeng,Xu Ziyao,Cao Junfeng,Gan Tiansheng,Wang Ben,Zhou Xuechang
Soft electronics are rising electronic technologies towards applications spanning from healthcare monitoring to medical implants. However, poor adhesion strength and significant mechanical mismatches inevitably cause the interface failure of devices. Herein we report a self-adhesive conductive polymer that possesses low modulus (56.1-401.9 kPa), high stretchability (700%), high interfacial adhesion (lap-shear strength >1.2 MPa), and high conductivity (1-37 S/cm). The self-adhesive conductive polymer is fabricated by doping the poly(3,4-ethylenedioxythiophene): poly(styrene sulfonate) composite with a supramolecular solvent (β-cyclodextrin and citric acid). We demonstrated the solution process-based fabrication of self-adhesive conductive polymer-based electrodes for various soft devices, including alternating current electroluminescent devices, electromyography monitoring, and an integrated system for the visualization of electromyography signals during muscle training with an array of alternating current electroluminescent devices. The self-adhesive conductive polymer-based electronics show promising features to further develop wearable and comfortable bioelectronic devices with the physiological electric signals of the human body readable and displayable during daily activities.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-12
·
IF 17.694
Zhang Yuan,Yang Junlong,Hou Xingyu,Li Gang,Wang Liu,Bai Ningning,Cai Minkun,Zhao Lingyu,Wang Yan,Zhang Jianming,Chen Ke,Wu Xiang,Yang Canhui,Dai Yuan,Zhang Zhengyou,Guo Chuan Fei
Electronic skins (e-skins) are devices that can respond to mechanical stimuli and enable robots to perceive their surroundings. A great challenge for existing e-skins is that they may easily fail under extreme mechanical conditions due to their multilayered architecture with mechanical mismatch and weak adhesion between the interlayers. Here we report a flexible pressure sensor with tough interfaces enabled by two strategies: quasi-homogeneous composition that ensures mechanical match of interlayers, and interlinked microconed interface that results in a high interfacial toughness of 390 J·m −2 . The tough interface endows the sensor with exceptional signal stability determined by performing 100,000 cycles of rubbing, and fixing the sensor on a car tread and driving 2.6 km on an asphalt road. The topological interlinks can be further extended to soft robot-sensor integration, enabling a seamless interface between the sensor and robot for highly stable sensing performance during manipulation tasks under complicated mechanical conditions.
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Scientific Reports
·
2019
·Vol. 9
·期 1
·1-10
·
IF 4.011
Sun Xiangyu,Cheng Cheng,Zhang Jinxie,Jin Xing,Sun Shuqing,Mei Lin,Huang Laiqiang
3-hydroxybutyrate-co-3-hydroxyhexanoate (PHBHHx), which is naturally generated by biodegradable polyhydroxyalkanoates synthesized by bacteria, is an attractive material for drug delivery due to its controllable physical properties, non-toxicity, environmental friendliness, degradable properties and good biocompatibility. However, the intracellular trafficking network pathways, especially the autophagy mechanism of PHBHHx nanoparticles (NPs), have rarely been investigated. In this paper, we successfully prepared the NPs used solvent displacement method and investigated the autophagy pathways and other intracellular trafficking mechanisms based on NPs with the assistance of Rab proteins. We found that NPs were internalized in cells mainly via clathrin endocytosis and caveolin endocytosis. Beside the classical pathways, we discovered two new pathways: the micropinocytosis early endosome (EEs)-micropinocytosis-lysosome pathway and the EEs-liposome-lysosome pathway. NPs were delivered to cells through endocytosis recycling vesicles and GLUT4 exocytosis vesicles. Similar to other nanoparticles, NPs also induced intracellular autophagy and were then degraded via endolysosomal pathways. 3-MA and CQ were used as autophagy inhibitors to avoid the degradation of NPs through lysosomes by blocking endolysosomal pathways. Tumor volumes and weights were significantly decreased when autophagy inhibitors and chemical drugs packaged in NPs were cooperatively used.
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Nature Communications
·
2020
·Vol. 11
·期 1
·1-15
·
IF 12.121
Cao Ji,Chen Xiaobing,Jiang Li,Lu Bin,Yuan Meng,Zhu Difeng,Zhu Hong,He Qiaojun,Yang Bo,Ying Meidan
Ferroptosis is a newly characterized form of regulated cell death mediated by iron-dependent accumulation of lipid reactive oxygen species and holds great potential for cancer therapy. However, the molecular mechanisms underlying ferroptosis remain largely elusive. In this study, we define an integrative role of DJ-1 in ferroptosis. Inhibition of DJ-1 potently enhances the sensitivity of tumor cells to ferroptosis inducers both in vitro and in vivo. Metabolic analysis and metabolite rescue assay reveal that DJ-1 depletion inhibits the transsulfuration pathway by disrupting the formation of the S-adenosyl homocysteine hydrolase tetramer and impairing its activity. Consequently, more ferroptosis is induced when homocysteine generation is decreased, which might be the only source of glutathione biosynthesis when cystine uptake is blocked. Thus, our findings show that DJ-1 determines the response of cancer cells to ferroptosis, and highlight a candidate therapeutic target to potentially improve the effect of ferroptosis-based antitumor therapy.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-8
·
IF 4.259
Chen Qijing,Shi Ting,Han Fei,Li Zihan,Lin Chao,Zhao Peng
A hydrophobic CCS polymer of poly(benzyl methacrylate) (PBzMA) was prepared in toluene by reversible addition-fragmentation chain transfer (RAFT)-mediated dispersion polymerization. The CCS polymer, with poly(benzyl methacrylate) as the arm and crosslinked N , N ′-bis(acryloyl)cystamine (BAC) as the core, was confirmed by characterization with gel permeation chromatography (GPC) and nuclear magnetic resonance (NMR) spectroscopy. Three kinds of oils (toluene, anisole and styrene) were chosen to study the emulsification properties of PBzMA CCS polymer. The oils can be emulsified by CCS polymer to form water-in-oil (w/o) emulsions. Moreover, w/o high internal phase emulsions (HIPEs) can be obtained with the increase of toluene and styrene volume fractions from 75% to 80%. Porous polystyrene monolith and microparticles were prepared from the emulsion templates and characterized by the scanning electronic microscopy (SEM). With the internal phase volume fraction increased, open-pore porous monolith was obtained.
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