CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
·
2021
·Vol. 99
·期 12
·
IF 2.273
Weimin Xu,Peng Wang,Dalong Wang,Ke Liu,Shuaishuai Zhang,Wei Zhao,Guoqing Liu
The aim of the present study was to investigate the protective effect of S-ketamine (S-KET) against carbon tetrachloride (CCl4) – induced liver damage and oxidative stress, as well as to elucidate the related underlying mechanisms. Blood was collected to measure biochemical parameters (alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total bilirubin (TB) and γ-glutamyltransferase (γ-GT)) and the liver was harvested for histopathological analysis of enzymes related to the antioxidant response (malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-PX)). Liver cell apoptosis was evaluated using the TUNEL assay. In addition, the expression levels of apoptosis-related proteins and the nuclear factor erythroid 2–related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway were detected by Western blot analysis to explore potential mechanisms. S-KET protected the liver from CCl4-induced damage. The changes to the liver biochemical parameters (increased ALT, AST, ALP, TB, and γ-GT) and oxidative stress-related indicators (increased MDA; depleted SOD, GSH, and GSH-PX) induced by CCl4 were inhibited by S-KET. S-Ket also inhibited CCl4-induced cell apoptosis, the changes in expression of related proteins, and blocked CCl4-induced liver injury and oxidative stress via activation of the Nrf2/HO-1 signaling pathway. S-KET effectively protected the liver by inhibition of CCl4-induced damage via upregulation the Nrf2/HO-1 signaling pathway.
✓ 已验证
DOI
PubMed
高影响因子
KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2016
·Vol. 33
·期 7
·2097-2106
·
IF 1.408
Bai Ting,Zhang Xin,Liu Xiling,Chen Tengfei,Fan Wentao
A series of HZSM-5 catalysts were synthesized by different methods. The physicochemical properties of the HZSM-5 catalysts were characterized by XRD, SEM, N2 isothermal adsorption-desorption, NH3-TPD, Py-IR and TGA, respectively. The results indicated that different preparation conditions lead to different morphologies, textures and the distribution of acid sites. The nanosized HZSM-5 catalysts exhibited better catalytic reactivity and coke capacity than the micro-sized HZSM-5 because nanosized HZSM-5 had larger specific surface area, higher pore volume, more exposed channels and more accessible acid sites. The large particles of NZ-3 in a reasonable range and the smooth surface were conducive to product diffusion; therefore, NZ-3 exhibited higher specific propylene yield and stability than the other nanosized catalysts. The moderate density and distribution of acid sites on NZ-3 also favored the formation of propylene.
✓ 已验证
DOI
高影响因子
Science of Advanced Materials
·
2016
·Vol. 8
·期 5
·1145-1153
·
IF 1.812
Ma Wang,Yi Shengen,Lin Liangwu,Huang He,Peng Tao,Liu Zhongtao,Liu Zhipeng,Pei Dongni,Miao Xiongying,Wen Yu,Chen Wei,Xiong Li
To synthesize a novel photosensitizer loaded into hollow titanium dioxide nanoparticles (HTNP) and evaluate the efficacy of its anticancer effects on Panc-1 pancreatic cancer cells in vitro. DLS measurements showed that the size of the particles ranged from 80 to 1,000 nm, with
an average diameter of 120 nm. The PDI of the Photosan-loaded HTNP was 0.112 ± 0.01, indicating that the samples have excellent quality with a narrow size distribution in monomodal systems. The photodynamic effects were positively related to the photosensitizer concentration, the light
dose, and the incubation duration. Using the same parameters, photodynamic therapy induced with Photosan-loaded HTNP had a more severe inhibition effect on cancer cell growth than that induced using free Photosan. Moreover, the nano-Photosan groups produced effects within a shorter duration
than free Photosan. Neither the Photosan-loaded nanoparticles nor free Photosan affected H6C7 cells cultured from a normal pancreas. The flow cytometry results demonstrated that laser-induced cell death with Photosan-loaded HTNP was substantially more severe than that induced with free Photosan.
Photosan-loaded HTNP are safe and stable and exhibit faster and more effective tumoricidal effects than free Photosan in the inhibition of Panc-1 cell growth.
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Zhiwen Ge,Xuan Bao,Zhiyu Li,Xiaohong Chen,Wei Li,Xin Rui,Junjun Wu,Qiuqin Zhang,Mingsheng Dong
In order to study the mechanism of high viscosity of Sayram ketteki yoghurt, the growth, acidification properties, in situ exopolysaccharides (EPS) production of Lactobacillus helveticus MB2-1 in milk medium were investigated. The microstructure of the yoghurt was analyzed. The characteristics of in situ EPS produced by this strain in yoghurt were studied by high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FT-IR), ultraviolet-visible (UV–vis) analysis. The amount of in situ EPS produced could be up to 689.47 mg/L. The micrographs of Sayram ketteki yoghurt demonstrated that the in situ EPS secreted by ropy L. helveticus MB2-1 were closely connected with proteins, effectively filling the three-dimensional network structure of casein clusters, thereby resulting in high viscosity of yoghurt. Besides, the molecular weight of in situ EPS was 9.34 × 10 4 Da, and the in situ EPS was determined to be a new heteropolysaccharide, containing fucose, which made it unique. Moreover, the set yoghurts added with in situ EPS were demonstrated fine effects on the texture improvement. These results illustrated that L. helveticus MB2-1 could be set as a good starter and the in situ EPS could be considered as a probiotic stabilizer substitute for fermented dairy products.
✓ 已验证
DOI
PubMed
高影响因子
Journal of Pharmaceutical Analysis
·
2022
·
IF 14.026
Liang Qi,Jiayun Zhang,Ying Gao,Pin Gong,Chengyuan Liang,Yao Su,Qiao Zeng,Yafeng Zhang
The regulator of expression of virion (Rev) protein binds specifically to the Rev-responsive element (RRE) RNA in order to regulate the expression of the human immunodeficiency virus (HIV)-1 genes. Fluorescence indicator displacement assays have been used to identify ligands that can inhibit the Rev–RRE interaction; however, the small fluorescence indicators cannot fully replace the Rev peptide or protein. As a result, a single rhodamine B labeled Rev (RB-Rev) model peptide was utilized in this study to develop a direct and efficient Rev–RRE inhibitor screening model. Due to photon-induced electron transfer quenching of the tryptophan residue on the RB fluorophore, the fluorescence of RB in Rev was weakened and could be dramatically reactivated by interaction with RRE RNA in ammonium acetate buffer (approximately six times). The interaction could reduce the electron transfer between tryptophan and RB, and RRE could also increase RB fluorescence. The inhibitor screening model was evaluated using three known positive Rev–RRE inhibitors, namely, proflavin, 6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine (ICR 191), and neomycin, as well as a negative drug, arginine. With the addition of the positive drugs, the fluorescence of the Rev–RRE decreased, indicating the displacement of RB-Rev. This was confirmed using atomic force microscopy (AFM) and the fluorescence was essentially unaffected by the addition of arginine. The results demonstrated that RB-Rev can be used as a fluorescent probe for recognizing small ligands that target RRE RNA. The Rev–RRE inhibitor screening model offers a novel approach to evaluating and identifying long-acting Rev inhibitors.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Jingming Wang,Bin Wu,Na Zhang,Mingyue Zhao,Tingting Jing,Yi Wu,YunQing Hu,Feng Yu,Xiaochun Wan,Wilfried Schwab,Chuankui Song
β-Ionone is a carotenoid-derived flavor and fragrance compound with a complex fruity and woody scent, known for its violet aroma. Due to the low odor threshold, β-ionone dramatically affects the aroma and quality of tea. Previous studies have shown that β-ionone increases during tea withering; however, its formation and regulation during the withering process are far from being understood. As dehydration is the most important stress during the withering of the tea leaves, we isolated a dehydration-induced gene belonging to the subfamily of carotenoid cleavage dioxygenases called carotenoid cleavage dioxygenase 1a (CsCCD1a) from Camellia sinensis and expressed it in Escherichia coli. The recombinant protein could generate volatile β-ionone and pseudoionone from carotenoids. CsCCD1a was induced by dehydration stress, and its expression was related to the β-ionone accumulation during tea withering. Overall, this study elucidated that CsCCD1a catalyzes the formation of β-ionone in C. sinensis and enhanced the understanding of the β-ionone formation under multiple stresses during the processing of tea.
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Yanna Lin,Xueying Wang,Yuanling Sun,Yuxue Dai,Weiyan Sun,Xiaodong Zhu,Hao Liu,Rui Han,Dandan Gao,Chuannan Luo
In this work, target-responsive DNA hydrogel with high loading and good biocompatibility was used to construct chemiluminescent biosensor for specific detection of adenosine. Acrydite-modified adenosine aptamer (Acry-A-Apt), acrydite-modified single-strand DNA (Acry-ssDNA) and hemin aptamer (H-Apt) were used to prepared the DNA hydrogel, and the three single-strand DNA were partially complementary to each other. Thus, the DNA hydrogel only dissolved when adenosine and hemin were present simultaneously. HKUST-1 is a Cu-based MOFs with peroxidase-like activity. Au NPs were coated into cavities of HKUST-1 to obtain [email protected] , and the [email protected] had a stronger peroxidase-like activity than HKUST-1. [email protected] was coated in the DNA hydrogel. When only hemin was present, hemin bound to the H-Apt to form the G-quadruplex/hemin and the DNA hydrogel remained in the gel state. Therefore, the G-quadruplex/hemin was formed in the hydrogel and the excess hemin can be removed, avoiding background signal interference. Adenosine was then added and the preferential interaction between adenosine and adenosine aptamer resulted in complete dissolution of the DNA hydrogel. Subsequently, G-quadruplex/hemin and [email protected] were released simultaneously, enabling dual signal amplification of chemiluminescent biosensor, yielding a “signal on” readout. As a result, the biosensor could quantify adenosine from 4 × 10 −13 – 1.5 × 10 -10 mol/L with a detection limit as low as 1.04 × 10 −13 mol/L. The DNA hydrogel system provides a simple, sensitive and biocompatible method for the detection of adenosine which is useful in the field of biotechnology.
✓ 已验证
DOI
高影响因子
DRUG DELIVERY
·
2020
·
IF 4.902
Pengcheng Zhang,He Tao,Liyang Yu,Lixiao Zhou,Chenggang Zhu
Injectable implants with the ability to form in situ are one of the most promising carriers for the delivery of chemotherapeutic drugs to tumor sites. We have reported a novel injectable in situ-forming implant system composed of n-butyl-2-cyanoacrylate (NBCA), ethyl oleate, along with the sol-gel phase transition. The chemotherapeutic drug-loaded injectable NBCA ethyl oleate implant (INEI) exhibited excellent therapeutic efficacy for local chemotherapy. Herein, we utilize this INEI to carry N, N′-(Sulfonyldi-4,1-phenylene)bis(2-chloroacetamide) (TE-C-5003), which is a selective protein arginine methyltransferase 1 (PRMT1) inhibitor, to treat the lung cancer mice model. The in vitro experiment shows that TE-C-5003 has a good anti-tumor effect on lung cancer (IC50 = 0.7022 µM for A549; IC50 = 0.6844 µM for NCL-H1299) and breast cancer (IC50 = 0.4128 µM for MCF-7; IC50 = 0.5965 µM for MDA-MB-231). Anti-tumor experiments in animal models showed that the average growth inhibition rate of xenografted human lung cancer cells by the TE-C-5003-loaded INEI (40% NBCA) was 68.23%, which is far more than TC-E-5003 alone (31.76%). Our study further confirms that INEI is an effective technique to improve the anti-tumor effect. The druggability of small molecule compounds can be improved with the help of the mentioned technology. Also, TC-E-5003 may be developed as a broad spectrum anti-tumor drug.
✓ 已验证
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2020
·
IF 4.831
Xiaogang Peng,Yangzhou He,Ying Gao,Fangfang Duan,Juan Chen,Hanli Ruan
Nine new ( 1 – 9 ) and four known ( 10 – 13 ) [13]cytochalasins, along with three known 24-oxa[14]cytochalasins ( 14 – 16 ), were isolated from the culture of Phoma multirostrata XJ-2-1, an endophytic fungus obtained from the fibrous root of Parasenecio albus . Their structures were elucidated by interpretation of the nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectroscopy (HRESIMS). The absolute configurations were assigned by single-crystal X-ray crystallography, modified Mosher′s method, and by analysis of their experimental electronic circular dichroism (ECD) spectra. Compound 6 could induce cell cycle arrest at G2-phase in CT26 and A549 cells, and displayed moderate cytotoxicity against CT26 and A549 cell lines with IC 50 values of 6.03 and 5.04 μM, respectively. Co-treatment of 7 – 9 , 13 and 16 with tumor necrosis factor related apoptosis inducing ligand (TRAIL) could significantly decrease the cell viability of A549, which revealed that cytochalasins could possibly be a new group of TRAIL sensitizers in lung cancer therapy.
✓ 已验证
DOI
PubMed
高影响因子
Biomolecules
·
2020
·Vol. 10
·期 6
·825
·
IF 4.082
Yazhen Hu,Tomofumi Kurobe,Xiaoling Liu,Yong-An Zhang,Jianguo Su,Gailing Yuan
Hepcidin is an antimicrobial peptide and regulator of iron homeostasis which has two isoforms in most fishes and some mammals. Previous studies have reported that the two hepcidin isoforms have different roles. Hamp type-1 plays a regulatory role in iron metabolism and hamp type-2 mostly performs an antimicrobial role. In this study, we found thatCtenopharyngodon idella(C. idella) have only one hepcidin isoform (hamp type-1), which showed both broad-spectrum antibacterial and iron regulatory functions.C. idellahepcidin mature peptide (hepcidin-25) and truncated peptide (hepcidin-20) exhibited bactericidal activities against both Gram-positive and Gram-negative bacteria in a dose-dependent manner in part through membrane rupture and binding to bacterial genomic DNA. The data from challenge tests demonstrated that the administration of hepcidin-25 significantly reduced mortality rates ofC. idellabyA. hydrophilainfection, probably due to direct bactericidal activities of the peptide and a reduction of iron content in the fish serum. In addition, a comparison between hepcidin-20 and -25 suggests that the N terminal 5 amino acids play a critical role in reducing iron content in fish serum. Our findings revealed an important role of hamp type-1 in maintaining iron homeostasis and fighting against bacterial infections, suggesting the hepcidin has implications for the prevention and control of bacterial infection in aquaculture.Keywords:antibacterial;Ctenopharyngodon idella;hepcidin;host defense;iron homeostasis
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Ruo-Nan Zhao,Zhe Feng,Ya-Nan Zhao,Li-Ping Jia,Rong-Na Ma,Wei Zhang,Lei Shang,Qing-Wang Xue,Huai-Sheng Wang
In this work, an ultrasensitive aptasensor for the detection of Mucin 1 (MUC1) was presented based on the target-induced catalytic hairpin assembly combined with excellent mimic peroxidase performance of PtPd bimetallic nanoparticles (PtPdNPs). Traditionally, the cyclic reuse of target protein was achieved by protein conversion with enzyme cleavage or polymerization, which is costly and complex. However, in this work, it can be performed by simple strand displacement. In addition, PtPdNPs, a mimic peroxidase, was used a probe to catalyze the oxidation of tetramethylbenzidine (TMB) by H 2 O 2 , leading to the electrochemical signal amplification. With this ingenious design, the prepared aptasensor for MUC1 detection showed a favorable linear response from 100 fg mL −1 to 1 ng mL −1 and a relatively low detection limit of 16 fg mL −1 . The proposed biosensor possessed acceptable stability, selectivity and reproducibility for MUC1 assay. Additionally, the fabricated aptasensor has been successfully applied to detect MUC1 in serum samples with satisfactory results. This new strategy supplied one efficient approach to improve signal amplification, which also open an avenue for sensitivity enhancement in targets detection.
✓ 已验证
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2012
·
IF 2.367
Jing-Yun Wang,Cui-Li Ma,Yong-Ming Bao,Pin-San Xu
Lipase from Burkholderia cepacia was encapsulated inside zirconia particles by biomimetic mineralization of K 2 ZrF 6 induced with protamine, a natural cationic protein. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FT-IR) were employed for the characterization of the novel immobilized lipase. SEM and TEM images showed that both the zirconia particles with and without lipase have good spherical structures with average particle sizes of 150 nm. Fluorescence microscopy demonstrated that the lipase was indeed encapsulated inside the zirconia particles. The maximum immobilization capacity of the zirconia particles was 0.15 units/mg under optimum immobilization conditions. Biochemical characterization showed that the encapsulated lipase could retain most of its initial activity. Compared with free lipase, the encapsulated lipase exhibited improved thermal, pH, and recycling stabilities. After 8 weeks of storage, no substantial loss in catalytic activity was observed for the encapsulated lipase. The conversion of the kinetic resolution of ( R,S )-1-phenylethanol with vinyl acetate as acetyl donor catalyzed by zirconia-immobilized lipase reached 49.9% with higher ee s of 99.9% under the following optimal conditions: octane as solvent, 0.1 M ( R,S )-1-phenylethanol, 70 mg immobilized lipase, 180 rpm, 50 °C for 48 h. After 6 cycles (288 h), the conversion and ee s were still 43% and 85%, respectively.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）