CHEMOSPHERE
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2023
·
IF 8.8
Qinghong Wang,Yayan Tong,Yalin Wu,Shuangying Li,Huahua Bai,Qingxiang Zhou
Bisphenols (BPs) are typical endocrine disruptors , which can cause great effects on environmental, organisms and human health. In this study, β-Cyclodextrin (β-CD) functionalized polyamidoamine dendrimers-modified Fe 3 O 4 nanomaterials (MNPs@PAMAM (G3.0)@β-CD) were facilely synthesized. It exhibited good adsorption capacities for BPs, which was utilized to construct a sensitive tool in combination with high performance liquid chromatography for monitoring BPs such as bisphenol A (BPA), tetrabromobisphenol A (TBBPA), bisphenol S (BPS), bisphenol AF (BPAF) and bisphenol AP (BPAP) in beverage samples. The factors affecting the enrichment were examined such as generation of adsorbent, dosage of adsorbent, type and volume of eluting solvent, elution time and pH value of sample solution. The optimal parameters for enrichment was as follows: dosage of adsorbent, 60 mg; adsorption time, 50min; sample pH, pH7; elutent, 9 mL mixture of methanol and acetone(1:1); elution time, 6min; sample volume, 60 mL. The experimental results demonstrated that the adsorption conformed to pseudo-second-order kinetic model and Langmuir adsorption isotherm model. The results showed the maximum adsorption capacities of BPS, TBBPA, BPA, BPAF and BPAP were 131.80 μgg −1 , 139.84 μgg −1 , 157.08 μgg −1 , 142.11 μgg −1 and 134.23 μgg −1 , respectively. Under optimal conditions, BPS had good linear relationship over range from of 0.5–300 μgL −1 , and the linear ranges of BPA, TBBPA, BPAF and BPAP ranged from 0.1 to 300 μgL −1 . The limits of detection (S/N = 3) for BPs were good in range of 0.016–0.039 μgL −1 . The spiked recoveries of target bisphenols (BPs) in beverages were approving over range from 92.3% to 99.2%. The established method possessed merits of easy to operate, good sensitivity, rapidness as well as environmental friendliness, and which earned great application potential for the enrichment and detection of trace BPs in practical samples.
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ELECTROPHORESIS
·
2023
·
IF 2.9
Yuanli Dai,Chang Sun,Yunhe Yang,Shuanghao Wang,Huihui Li,David Da Yong Chen
The thermodynamic properties of molecular recognition in host–guest inclusion complexes can be studied by Taylor dispersion analysis (TDA). Host–guest inclusion complexes have modest size, and it is possible to get convergent results fast, achieving greater certainty for the obtained thermodynamic properties. Cyclodextrins (CDs) and their derivatives can be used as drug carriers that can boost stability, solubility, and bioavailability of physiologically active substances. A simple and effective approach for assessing the binding properties of CD complexes that are critical in the early stages of drug and formulation development is needed to fully understand the process of CD and guest molecules’ complex formation. In this work, TDA was successfully used to rapidly determine interaction parameters, including binding constant and stoichiometry, between β-CD and folic acid (FA) along with the diffusivities of the free FA and its complex with β-CD. Additionally, the FA diffusion coefficient obtained by TDA was compared to the results previously obtained by nuclear magnetic resonance. Affinity capillary electrophoresis (ACE) was also used to compare the binding constants obtained by different methods. The results showed that the binding constants obtained by ACE were somewhat lower than those obtained by the two TDA procedures.
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Arabian Journal of Chemistry
·
2023
·
IF 6
Bocong Li,Guoqiang Xiang,Gao Huang,Xiuming Jiang,Lijun He
A rapid, green synthesis strategy assisted by a self-exothermic reaction was designed to prepare highly luminescent carbon dots (CDs) from Ganpu tea infusion (G-CDs) within 5 min using H 2 O 2 and diethylenetriamine (DETA) as the exothermic reactants. G-CDs were successfully obtained through a self-exothermic reaction The prepared G-CDs exhibited clear excitation-dependent characteristics and displayed a strong ultraviolet fluorescence emission (385 nm) at the excitation wavelength of 320 nm. The fluorescence of G-CDs was quantitatively quenched by para -nitrophenol (p-NP) with a concentration range of 0.05–50 µM through inner filter effect (IFE). Since p-NP is one of the hydrolysates of the substrate pNPG under the action of Beta-glucosidase (β-Glu), a new fluorescence assay was developed for the indirect determination of β-Glu activity. The detection limits of G-CDs based fluorescence assays are 14.6 nM for p-NP and 0.074 U·L -1 for β-Glu activity, respectively. The developed fluorescence assays were successfully applied to the detection of p-NP in water and human urine samples and β-Glu activity in fruit samples with satisfactory results.
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FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Xin Liu,Fang Xie,Jiaojao Zhou,Jiangling He,Zia-ud Din,Shuiyuan Cheng,Jie Cai
Food-grade compound-stabilized high-internal-phase Pickering emulsions (HIPEs) have received widespread attention in food industry and functional foods. However, challenges are still there regarding the inclusion of active substances and quickly processing molding for food manufacturing. Herein, we used zein , tannic acid (TA) and sodium alginate (SA) to synergistically increase the stability of HIPEs. Owing to electrostatic, hydrophobic and hydrogen-bond interactions among protein, polyphenol and polysaccharide , Zein-TA-SA composite particles-stabilized HIPEs (ZTS-HIPEs) exhibited good pH and ionic strength stability. The HIPEs could further be used to load and solubilize β-carotene, while significantly improved the retention of β-carotene in different environments. Simulated in vitro digestion experiments showed that the release rate of free fatty acids and bioaccessibility of β-carotene in HIPEs were significantly improved, indicating that HIPEs might achieve efficient delivery of β-carotene in the gastrointestinal environment. In addition, the ZTS-HIPEs could be 3D printed as food-grade inks due to their good rheological properties as the printed models showed good precision and stability. In summary, the ZTS-HIPEs prepared in this study could be used not only as an effective delivery carrier of β-carotene, but also as a potential ink for 3D printing. The research findings in this study are expected to provide novel insight on HIPEs for nutrition delivery systems and future food manufacturing.
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Frontiers in Pharmacology
·
2023
·
IF 5.6
Liu Guanjie,Chang Ling,Qian Yihan,Lin Jiacheng,Shang Zhi,Xu Min,Wang Fang,Sun Xuehua,Jiang Yun,Gao Yueqiu,Kong Xiaoni
Non-alcoholic fatty liver disease (NAFLD) is a predominant contributor to end-stage liver disease in the forthcoming decades. Polygonum perfoliatum L. (PPL) is an herbal medicine with anti-lipid peroxidation and anti-inflammatory properties. However, detailed hepatoprotective effects of PPL against NAFLD and its underlying mechanisms are not fully understood. Here, we found that PPL protects against high fat diet (HFD)-induced hepatic steatosis, lipid peroxidation, and glucose-lipid metabolism dysfunction in NAFLD mice. We therefore performed a label-free quantitative proteomic profiling analysis to determine the effect of PPL treatment on liver tissue proteomics and identified that activated PPARs/CPT1A/CPT2-mediated hepatic fatty acid β-oxidation (FAO) process was significantly altered. In vitro treatment of hepatocytes with PPL confirmed this altered process and FAO inhibitor etomoxir (ETO) attenuated the lipid-lowering activity of PPL in hepatocytes. Ultra-high-performance liquid chromatography/Q Exactive-HFX (UPLC/QE-HFX) was used to determine the material basis of anti-NAFLD activity of PPL. Our results have demonstrated the efficacy and potential mechanisms of PPL as an effective pharmacological therapy of NAFLD.
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FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Yong Xie,Qingqing Liu,Haibo Liu,Zhiping Fan,Jia Shi,Xiong Liu
The favorable prebiotic effect of cereal β-glucans is associated with its structure characteristic, which may be influenced by thermal processing. This study aimed to evaluate the structure and in vitro fermentation characteristics of β-glucan isolated from hulless barley preprocessed with flaking-roasting (FR), stir-frying (SF), steam-flash explosion (SFE) and popping expansion (PE). The results showed that all thermal processing had no impact on the type of chemical groups. Compared with the native β-glucan, the molecular weight of PE-β-glucan increased from 147.36 × 10 3 Da to 5, 243.60 × 10 3 Da, whereas its crystallinity index decreasing from 52.9 ± 0.7% to 41.5 ± 1.1%. Furthermore, the PE-β-glucan exhibited a porous structure like that in native β-glucan, while the SFE-β-glucan displayed a compacted structure with the lowest molecular weight (89.90 × 10 3 Da). Expectedly, PE-β-glucan exerted the highest availability and increased the relative abundance of Bifidobacterium , while FR-, SF-, and SFE-β-glucan tended to promote the growth of Megasphaera and Lactobacillus . Additionally, the level of total SCFAs, acetate, propionate , and butyrate in PE-β-glucan were dramatically higher than those in other β-glucan. Overall, the chain conformation of β-glucan played a pivotal role in determining its bioavailability and probiotic characteristic, which could be improved evidently by PE processing.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xinyi Xing,Xian Shi,Mengya Ruan,Qichun Wei,Ying Guan,Hui Gao,Siquan Xu
Introduction of the sulfonic acid group into H-β zeolite to prepare β-SO 3 H bifunctional catalysts for the efficient synthesis of 5-hydroxymethylfurfural (HMF) from cellulose . Catalysts characterization, such as XRD, ICP-OES, SEM (Mapping), FTIR, XPS , N 2 adsorption-desorption isotherm, NH 3 -TPD, Py-FTIR demonstrate the sulfonic acid group was successfully grafted onto the β zeolite. A superior HMF yield (59.4 %) and cellulose conversion (89.4 %) was obtained in the H 2 O(NaCl)/THF biphasic system under 200 °C for 3 h with β-SO 3 H(3) zeolite as catalyst. More valuable, β-SO 3 H(3) zeolite converts other sugars and obtains ideal HMF yield, including fructose (95.5 %), glucose (86.5 %), sucrose (76.8 %), maltose (71.5 %), cellobiose (67.0 %), starch (68.1 %), glucan (64.4 %) and also converts plant material (25.1 % for moso bamboo and 18.7 % for wheat straw) with great HMF yield. β-SO 3 H(3) zeolite catalyst keeps an appreciable recyclability after 5 cycles. Moreover, in the presence of β-SO 3 H(3) zeolite catalyst, the by-products during the production of HMF from cellulose were detected, and the possible conversion pathway of cellulose to HMF was proposed. The β-SO 3 H bifunctional catalyst has excellent potential for the biorefinery of high value platform compound from carbohydrates.
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NEUROSCIENCE
·
2022
·
IF 3.708
Chunyan Chen,Ping Fan,Lirong Zhang,Kaige Xue,Jiaheng Hu,Juan Huang,Weitian Lu,Jin Xu,Shiye Xu,Guoping Qiu,Jianhua Ran,Shengwei Gan
Aquaporin-4 (AQP4) regulates retinal water homeostasis and participates in retinal oedema pathophysiology. β-dystroglycan (β-DG) is responsible for AQP4 polarization and can be cleaved by matrix metalloproteinase-9 (MMP9). Retinal oedema induced by ischemia–reperfusion (I/R) injury is an early complication. Bumetanide (BU) has potential efficacy against cytotoxic oedema. Our study investigated the effects of β-DG cleavage on AQP4 and the roles of BU in a rat retinal I/R injury model. The model was induced by applying 110 mm Hg intraocular pressure to the anterior eye chamber. BU and U0126 (a selective ERK inhibitor) were intraperitoneally administered 15 and 30 min, respectively, before I/R induction. Rhodamine isothiocyanate extravasation detection, quantitative real-time PCR, transmission electron microscopy, hematoxylin-eosin staining, immunofluorescence staining, western blotting, and TUNEL staining were performed. AQP4 lost its polarization in the retinal perivascular domain as a result of β-DG cleavage. BU rescued AQP4 depolarization, suppressed AQP4 protein expression, attenuated retinal cytotoxic oedema, and downregulated β-DG and AQP4 mRNA expression. BU suppressed glial responses and mitochondria-mediated apoptotic protein expression, including that of Caspase-3 and Cyto C, raised the Bcl-2/Bax ratio, and lowered the number of apoptotic cells in the retina. Both BU and U0126 downregulated p-ERK and MMP9 expression. Thus, BU treatment suppressed β-DG cleavage, recovered AQP4 polarization partially via inhibiting ERK/MMP9 signaling pathway, and possess potential neuroprotective efficacy in the rat retinal ischemia–reperfusion injury model.
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Polymers
·
2022
·Vol. 15
·期 1
·116
·
IF 4.967
Tisana Kaewruethai,Yuan Lin,Qian Wang,Jittima Amie Luckanagul
Curcumin is an extract of turmeric (Curcuma longa) which possesses anti-inflammatory, anti-cancer and wound-healing effects and has been used as an active compound in biomedical research for many years. However, its poor solubility presents challenges for its use in drug delivery systems. A modified nanogel delivery system, with PNIPAM and β-cyclodextrin grafted onto hyaluronic acid (PNCDHA), was utilized to enhance the solubility. The polymer was characterized by NMR, and the inclusion complex between curcumin and β-cyclodextrin was confirmed by FTIR. The potential of this PNCDHA polymer complex as a drug delivery vehicle was supported by a curcumin encapsulation efficiency of 93.14 ± 5.6% and the release of encapsulated curcumin at 37 °C. At a concentration of 0.5%w/vin water, PNCDHA nanogels were biocompatible with fibroblast cell line (L929) up to a curcumin concentration of 50 µM. There was a direct concentration between curcumin loading and cellular internalization. A more detailed study of the cellular internalization of PNCDHA nanogel should be considered in order to clarify cellular delivery mechanisms and to assess how its viability as a carrier may be optimized.Keywords:hyaluronic acid;β-cyclodextrin;PNIPAM;thermoresponsive;nanogel;self-assembly;curcumin
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ENZYME AND MICROBIAL TECHNOLOGY
·
2022
·
IF 3.705
Hai-Yan Zhou,Qi Chen,Yi-Feng Zhang,Dou-Dou Chen,Xiao-Nan Yi,De-Shui Chen,Xin-Ping Cheng,Mian Li,Hong-Yan Wang,Kai-Qian Chen,Zhi-Qiang Liu,Yu-Guo Zheng
In order to improve the degradation activity of β-glucosidase (CpBgl) from Coniophora puteana , the structural modification was conducted. The enzyme activity of mutants CpBgl-Q20C and CpBgl-A240S was increased by 65.75% and 58.58%, respectively. These mutants exhibited maximum activity under the same conditions as wild-type CpBgl (65 ℃ and pH 5.0), slightly improved stabilities compared that of the wild-type, and remarkably enhanced activities in the presence of Mn 2+ or Fe 2+ . The V max of CpBgl-Q20C and CpBgl-A240S was increased to 138.18 and 125.14 μmol/mg/min, respectively, from 81.34 μmol/mg/min of the wild-type, and the catalysis efficiency ( k cat / K m ) of CpBgl-Q20C (335.79 min −1 /mM) and CpBgl-A240S (281.51 min −1 /mM) was significantly improved compared with that of the wild-type (149.12 min −1 /mM). When the mutant CpBgl-Q20C were used in the practical degradation of different biomasses, the glucose yields of filter paper, corncob residue, and fungi mycelia residue were increased by 17.68%, 25.10%, and 20.37%, respectively. The spatial locations of the mutation residues in the architecture of CpBgl and their unique roles in the enzyme-substrate binding and catalytic efficiency were probed in this work. These results laid a foundation for evolution of other glycoside hydrolases and the industrial bio-degradation of cellulosic biomass in nature.
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Nanoscale
·
2023
·Vol. 15
·期 8
·4063-4070
·
IF 6.7
Mengjiao Li,Qingjie Yu,Meie Zheng,Rongrong Jiang,Hongda Zhu,Huiling Guo,Hongmei Sun,Mingxing Liu
A label-free electrochemical immunosensor for high-sensitive detection of β-amyloid 1–42 (Aβ 1–42) was constructed based on Au-modified B, S, and N co-doped reduced graphene oxide (Au-BSN-rGO). The electronic structure of Au-BSN-rGO was investigated by first-principles calculations, which showed that the band gap of graphene was opened, thus improving its electrical conductivity. Moreover, Au-BSN-rGO was successfully prepared and characterized, and the obtained results discovered that it could be used as a signal amplifier for immunosensors due to the advantages of the good electrochemical characteristics and enormous surface area of BSN-rGO and the accelerated electron transfer ability of Au NPs. Furthermore, the label-free electrochemical immunosensor had a linear detection range of 0.1 pg mL−1–10 ng mL−1 and a detection limit of 0.072 pg mL−1, and it had good specificity, stability, and reproducibility. Also, this immunosensor showed recoveries of 89%–109% with an RSD of 2.61%–4.19% for detecting Aβ 1–42 in actual sample analysis. Therefore, the label-free electrochemical immunosensor based on Au-BSN-rGO should have a promising clinical application prospect for detecting Aβ 1–42.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Qingzhao Shi,Guobi Chai,Xiujuan Xu,Wu Fan,Chaojun Wu,Lingbo Ji,Hui Xi,Jian Mao,Junhui Liu,Yubing Song,Qidong Zhang
The proportion of odorants has a key effect on the odor/flavor profile of perfumes or food products. However, direct encapsulation changes the odorant release characteristics, resulting in corresponding variations in their organoleptic qualities. In this study, nine key odorants (KOs) of Osmanthus absolute (OA) were identified for the aroma reconstruction of OA. Inclusion complexes of β-cyclodextrin (β-CD) with KOs (KO-β-CDs) were obtained via co-precipitation, the loading of KOs in β-CD was varied from 34.86 to 108.88 mg/g. Based on the aroma release behavior analyses, main aroma characteristics of OA were reconstructed from the KO-β-CDs via replication of the headspace KO distributions of OA. Aroma profile evaluation validated the effectiveness of aroma reconstruction. Besides, the release characteristics of directly encapsulated OA (OA-β-CD) and OA were found to significantly differ. For example, linalool oxide furanoid contributed greater than 20% of the total KOs in the headspace of OA, contributed only 8.89% in the headspace of the OA-β-CD. Further, the aroma intensity of the five main descriptors was reduced to different degrees. The KO-β-CDs mixture resembles the odor profile of OA to a greater degree than OA-β-CD. Our methodology provides a route to maintain the organoleptic quality of flavors in β-CD inclusion complexes.
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危险品化学品经营许可证（带存储）