Veterinary Sciences
·
2024
·Vol. 11
·期 3
·135
·
IF 2
Yiyi Shan,Qi Xiao,Kongwang He,Shenglong Wu,Wenbin Bao,Zhengchang Wu
Simple SummaryPorcine circovirus type 2 (PCV2) infection can cause immunosuppression in pigs, create conditions for other virus infections, and bring serious economic losses to the pig industry. 5-Azacytidine (5-Aza) is a potent inhibitor of DNA methylation. In this study, we focused on the effects of 5-Aza on PCV2 infection in porcine kidney cells (PK15 cells). We found that 5-Aza could activate cell apoptosis and inflammatory factors and promote PCV2 replication through the MAPK signaling pathway.AbstractPorcine circovirus type 2 (PCV2) is the main pathogen causing post-weaning multisystemic wasting syndrome (PMWS), which mainly targets the body’s immune system and poses a serious threat to the global pig industry. 5-Azacytidine is a potent inhibitor of DNA methylation, which can participate in many important physiological and pathological processes, including virus-related processes, by inhibiting gene expression. However, the impact of 5-Aza on PCV2 replication in cells is not yet clear. We explored the impact of 5-Aza on PCV2 infection utilizing PK15 cells as a cellular model. Our objective was to gain insights that could potentially offer novel therapeutic strategies for PCV2. Our results showed that 5-Aza significantly enhanced the infectivity of PCV2 in PK15 cells. Transcriptome analysis revealed that PCV2 infection activated various immune-related signaling pathways. 5-Aza may activate the MAPK signaling pathway to exacerbate PCV2 infection and upregulate the expression of inflammatory and apoptotic factors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2025
·
IF 4.5
Zhang Yue,Wang Xiang,Deng Duping,Gong Yuanyuan,Chen Xuanyi,Li Juan,Hong Xiaojuan
Objective: This study aimed to investigate the impact of electroacupuncture (EA) on blood glucose levels, gut microbiota, short-chain fatty acids (SCFAs), and glucagon-like peptide-1 (GLP-1) in a rat model of type 2 diabetes mellitus (T2DM).Methods: Forty Sprague–Dawley (SD) rats were randomly assigned to five groups (n = 8/group) using a random number table: normal control, T2DM model, electroacupuncture (EA), EA + antibiotics (EA + A), and antibiotics (A). The normal rats received a standard diet and saline gavage, while the other groups were fed a high-fat diet and emulsion. The EA + A and A groups received additional antibiotic solution gavage. The normal, model, and A groups were immobilized and restrained for 30 min, six times per week, for 4 weeks. The EA and EA + A groups received EA treatment at specific acupoints for 30 min, six times per week, for 4 weeks. EA parameters were continuous waves at 10 Hz and 1–2 mA. During the intervention, water and food consumption, body weight, fasting blood glucose (FBG), and oral glucose tolerance test (OGTT) were monitored. Pancreatic tissue was examined using hematoxylin and eosin (H&E) staining. Fecal microbial communities were analyzed by 16S rDNA sequencing, and short-chain fatty acids (SCFAs) were measured using gas chromatography–mass spectrometry (GC–MS). Serum levels of fasting insulin (FINS), glycated hemoglobin (HbA1c), and glucagon-like peptide-1 (GLP-1) were determined using enzyme-linked immunosorbent assay (ELISA).Results: EA significantly improved daily water intake, food consumption, and body weight in T2DM rats (p < 0.01). EA also reduced FBG, the area under the curve of the OGTT, FINS, and homeostasis model assessment of insulin resistance (HOMA-IR) in T2DM rats (p < 0.05). The ELISA results showed a lower concentration of HbA1c in the EA group (p < 0.05). EA improved the overall morphology and area of pancreatic islets, increased the number of β-cell nuclei, and alleviated β-cell hypertrophy. The abundance of operational taxonomic units (OTUs) in the EA group increased than the model group (p < 0.05), and EA upregulated the Shannon, Chao1, and Ace indices (p < 0.05). EA increased the concentrations of acetic acid, butyric acid, and GLP-1 (p < 0.05). Correlation analysis revealed negative associations between Lactobacillaceae (R = −0.81, p = 0.015) and Lactobacillus (R = −0.759, p = 0.029) with FBG. Peptostreptococcaceae and Romboutsia were negatively correlated with HbA1c (R = −0.81, p = 0.015), while Enterobacteriaceae was positively correlated with OGTT (R = 0.762, p = 0.028). GLP-1 was positively correlated with acetic acid (R = 0.487, p = 0.001), butyric acid (R = 0.586, p = 0.000), isovaleric acid (R = 0.374, p = 0.017), valeric acid (R = 0.535, p = 0.000), and caproic acid (R = 0.371, p = 0.018). Antibiotics disrupted the intestinal microbiota structure and weakened the therapeutic effects of EA.Conclusion: EA effectively improved glucose metabolism in T2DM rats. The hypoglycemic effects of EA were associated with the regulation of gut microbiota, SCFAs, and GLP-1.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AICHE JOURNAL
·
2024
·
IF 3.5
Guimei Zhou,Wenjun Yan,Shufan Yan,Dandan Gao,Debin Zeng,Dongdong Li,Dewen Zeng
Solvent extraction of lithium by β-diketones from alkaline brine has been known to be an efficient process. However, its relatively high working pH, consequently the high alkaline consumption and substantial dissolution loss in raffinate, limit its industrial application. Herein, a novel lithium extractant, i.e., 2-hydroxy-5-nitro-4- n -octoxy-benzophenone (referred to as N531), was proposed, which can extract lithium at relatively lower pH with remarkable low alkaline consumption and dissolution loss. Exactly, the pH corresponding to half lithium extraction is 8.5, and the Li/Na separation factor is ~500. The extractant concentration in the raffinate varied from 0.8 to 9.3 mg L −1 , depending on the pH and salinity. An application case was given to extract lithium from the raw brine of Zabuye salt lake, indicating that N531 is a commercially prospective extractant to extract lithium from alkaline brine.
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Renzhong Xue,Xiaosong Liu,Xiang Zhu,Tao Li,Haiyang Dai,Jing Chen
It is always a challenging problem to simultaneously obtain a large dielectric constant ( ɛ ′), low dielectric loss (tan δ ), and good nonlinear performance in the ACu 3 Ti 4 O 12 (ACTO) ceramic family. In this study, Y 3+ -doped Bi 2/3 Cu 3 Ti 4 O 12 ceramics with a giant ɛ ′, low tan δ , high breakdown field strength ( E b ), and high nonlinearity coefficient ( α ) were successfully prepared via the solid-state method. The (Bi 0.5 Y 0.5 ) 2/3 Cu 3 Ti 4 O 12 ceramic exhibited a huge ɛ ′ (∼12660), very small tan δ (∼0.019), high E b (∼15.7 kV/cm), high α (∼7.61), and high energy storage density (∼140.2 mJ/cm 3 ). The impedance spectra and electrical modulus results show that the dielectric properties of the prepared ceramics follow the internal barrier layer capacitor model. The enhanced tan δ and improved nonlinear properties of the doped samples are associated with the increased grain boundary resistance and Schottky barrier. These results provide important clues and valuable insights for realizing bifunctional ACTO materials with a giant ɛ ′, low tan δ , and excellent nonlinear properties.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Physical Chemistry C
·
2024
·
IF 3.3
Rong Sun,Xinle Lu,Minlu Zhou,Jiamin Yao,Pengzhen Chen,Wen-Yi Tong,Chun-Gang Duan,Jun Zhang
In this study, the self-assembly structures of 2-methylbenzimidazole (MBI) molecules on Cu(100) and Cu(111) surfaces were examined using scanning tunneling microscopy (STM), atomic force microscopy (AFM), and density functional theory (DFT) calculations. It was found that MBI molecules form hydrogen-bonded structures in one-dimensional (1D) or two-dimensional (2D) configurations on both Cu(100) and Cu(111) surfaces. On the Cu(100) surface, the hydrogen-bonded lines are aligned along two equivalent Cu ⟨11̅0⟩ directions, matching the lattice of the underlying surface. This alignment agrees with calculation results, which shows MBI molecules preferentially adsorbing on hollow sites with their molecular backbones perpendicular to these lines. The molecular lines spread out across the surface area, and the distance between them gradually decreases as coverage increases, indicating repulsive interactions between the lines. Conversely, on the Cu(111) surface, the 1D molecular chains are curly, with the molecular backbones oriented in various directions relative to the substrate, indicating a weaker interaction between the molecules and the substrate compared to that on the Cu(100) surface. With further increases in coverage on both surfaces, densely packed 2D molecular structures emerge, albeit with different lattice parameters. The 2D structures on Cu(100) adjust to match the substrate, whereas those on Cu(111) may assume various orientations. The distance between molecules is greater on Cu(111) than on Cu(100), suggesting a restriction of hydrogen bonding structures on the Cu(100) surface. Additionally, a noticeable height difference among the molecules in the 2D structures was observed, possibly due to a slight tilting of the molecular backbone when more molecules are integrated into the lattice. These findings demonstrate how the assembly structures of molecules depend on the interaction between molecule–molecule and molecule–substrate interactions.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2024
·
IF 4.1
Xin Zhang,Song Li,Jie Yuan,Zhongning Shi
The effect of magnesium chloride (MgCl 2 ) on the electrodeposition of zinc in 1,3-dimethyl-2-imidazolidinone (DMI)/ZnCl 2 solvate ionic liquids was investigated. Cyclic voltammetry and Chronoamperometry are employed to study the electrochemical behavior of Zn. The crystal structure, microscopic morphology, and roughness of Zn coatings were measured by X-ray diffraction (XRD), scanning electron microscopy (SEM), and Non-contact Optical Surface Profiler (OSP). The results reveal that adding MgCl 2 is favorable to the electrodeposition of Zn and leads to the formation of a new zinc-containing species in the electrolyte, which was confirmed by Raman spectra. Importantly, this addition does not affect the nucleation mechanism of zinc. Furthermore, the zinc grows preferentially on the (1 0 1) plane and a more smooth and uniform zinc coating was obtained. Corrosion resistance testing is conducted in a 3.5 wt% NaCl solution using the Tafel method. Coatings obtained at 4 mA/cm 2 and 343 K with 0.1 mol/L MgCl 2 addition exhibit lower corrosion current density of 38.10 μA/cm 2 compared to zinc coatings obtained without MgCl 2 .
✓ 已验证
产品关联
DOI
高影响因子
SOLAR ENERGY MATERIALS AND SOLAR CELLS
·
2024
·
IF 6.3
Lei Sun,Chu Zhang,Gence Bektaş,Xiaowei Li,Chunying Ma,Tingli Ma
The power conversion efficiency (PCE) of organic-inorganic halide perovskite solar cells (PSCs) developed rapidly in recent years. However, the defects at the bulk grain boundaries and heterojunction interfaces acting as non-radiative recombination centres and the ion-migration channels severely hinder the charge transport and stability of the PVKs, resulting in low PCE and poor stability. One of the principal impediments to the commercialization of PSCs resides in the challenge posed by this particular aspect. In this work, we employed 2-fluoroisonicotinic acid (2-FINA) as a passivation agent to improve the PCE and stability of n-i-p PSCs by interfacial modification strategy. Owing to the presence of carboxyl (COOH) functional groups and pyridine groups, 2-FINA can strongly interact with uncoordinated Pb 2+ and regulate the growth of perovskite crystals, which in turn reduces ion migration and suppress non-radiative recombination along with improves optical properties. Thanks to these improvements, the champion n-i-p PSC solar cell, treated with 2-FINA, showed a PCE of 20.92 %, whereas the untreated one in the same batch exhibited a PCE of 17.13 %. In long-term stability tests, we demonstrated that 2-FINA treatment significantly increases the moisture resistance of non-encapsulated devices.
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Bingke Yang,Haojie Li,Zhen Yao,Lili Xing,Xuetao Wang,Yilin Zhu
In the study, ammonia borane alcoholysis with different types of alcohols (CH3OH, CH3CH2OH, (CH2OH)2) for hydrogen production are investigated, and the effects of different pressures and temperatures on the adsorption properties are also investigated using the DFT calculation and experimental method. In the ammonia borane alcoholysis, NH3BH3 molecules and alcohols molecules (R-OH) are first adsorbed onto the active metal surface, where the hydroxyl groups in the R-OH molecules are activated and the O-H bonds are broken to produce the intermediates H* and *O-R. Further a strong interaction between the B in NH3BH3 and the O in *O-R occurs and the B-N bond is broken, forming the intermediate products *NH3, *BH2-O-R and another H*, H2 molecule is released during B-O bonding with one of the H originated from the hydroxyl group and the other originated from the *HB in the NH3BH3 molecule. The energy barriers for the ammonia borane alcoholysis with CH3OH, CH3CH2OH and (CH₂OH)₂ are 28.23 kcal/mol, 37.5 kcal/mol and 42.32 kcal/mol, respectively, which results in a different alcoholysis rate of ammonia borane AB/CH3OH> AB/CH3CH2OH> AB/(CH2OH)2 at the macroscopic level. The high pressure enhances the adsorption properties of ammonia borane and alcohol molecules, while the high temperature decreases the adsorption properties. The adsorption capacities of ammonia borane and alcohols on the active metal Ru (101) are CH3OH (0.32 mmol/g) < NH3BH3 (1.03 mmol/g) < CH3CH2OH (1.05 mmol/g) < (CH2OH)2 (1.31 mmol/g) specifically. The results of this study provide a theoretical basis for the regulation of hydrogen production from ammonia borane alcoholysis.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2024
·
IF 4.8
Shixiong Liu,Yun Zhou,Yarong Chen,Yuqin Qiao,Lumucao Bai,Shenhua Zhang,Dongfang Men,Haibu Zhang,Fen Pan,Yongshen Gao,Jijing Wang,Yuping Wang
Changes in isocitrate dehydrogenases (IDH) lead to the production of the cancer-causing metabolite 2-hydroxyglutarate, making them a cause of cancer. However, the specific role of IDH in the progression of colon cancer is still not well understood. Our current study provides evidence that IDH2 is significantly increased in colorectal cancer (CRC) cells and actively promotes cell growth in vitro and the development of tumors in vivo . Inhibiting the activity of IDH2, either through genetic silencing or pharmacological inhibition, results in a significant increase in α-ketoglutarate (α-KG), indicating a decrease in the reductive citric acid cycle. The excessive accumulation of α-KG caused by the inactivation of IDH2 obstructs the generation of ATP in mitochondria and promotes the downregulation of HIF-1A, eventually inhibiting glycolysis. This dual metabolic impact results in a reduction in ATP levels and the suppression of tumor growth. Our study reveals a metabolic trait of colorectal cancer cells, which involves the active utilization of glutamine through reductive citric acid cycle metabolism. The data suggests that IDH2 plays a crucial role in this metabolic process and has the potential to be a valuable target for the advancement of treatments for colorectal cancer.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2024
·Vol. 12
·期 26
·9652-9661
·
IF 5.7
Ling Zhang,Jie Yang,Junchao Yang,Jianan Wei,Chuan Zhou,Jiang Zhao,Qibin Huang
The fabrication of gas-sensing devices with high sensitivity and low limit of detection (LOD) for monitoring chemical warfare agents and their simulants is of great importance. Metal oxide-based semiconducting nanomaterials and their heterojunctions hold great promise in chemiresistive gas sensing. However, the demands of high working temperature, integration on interdigitated electrodes (IDEs) and requirement of additional heaters impede their popularity. This work demonstrates in situ laser-assisted preparation of Au-decorated laser-induced graphene (LIG/Au) hetero-material as a flexible and effective near-room-temperature 2-chloroethyl ethyl sulfide (2-CEES) sensor. The structure of the sensor was simplified via omitting the IDE and an additional heating module. After optimizing the concentration of the HAuCl4 precursor solution and power of secondary scribing, the LIG/Au-0.1 M gas sensor exhibited an enhanced response of 7.85‰ to 1.0 ppm 2-CEES, which is twice that of pure LIG. Furthermore, its LOD was as low as 5.8 ppb. The sensing mechanism analysis revealed that the decoration of Au nanoparticles enriched the active sites of LIG/Au-0.1 M and provided catalytic and sensitization effects. The present study not only provides an appealing 2-CEES gas sensor but also demonstrates an effective method for large-scale fabrication of other high-performance sensing devices.
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Xiaotian Zhang,Zipeng Jin,Kunting Li,Yisheng Hu,Lei Xu,Baiyan Li
Despite their high surface area, remarkable porosity, and efficient charge transfer mechanisms, conductive MOFs have found limited utilization within the domain of photocatalysis. In this study, we synthesized a cutting-edge S-type Ni3(HITP)2/TiO2 heterojunction photocatalyst exhibiting outstanding light harvesting and prolonged lifetime of photogenerated electrons through an in situ synthesis approach. Compared with TiO2, the composite materials not only significantly increase the specific surface area by 4.07 times but also expand the visible light absorption edge from 400 to 1100 nm. The hydrogen production rate of Ti/Ni-3 reached 4.927 mmol·g–1·h–1, which is 4.51 times that of TiO2. The S-type interface charge transfer pathway of the Ni3(HITP)2/TiO2 composite material was inferred by band structure, in situ XPS, SPV, and free radical capture, which improves charge separation and extends the carrier lifetime to undergo directional migration driven by an IEF. This is the main reason for the improved photocatalytic performance of Ni3(HITP)2/TiO2 composite materials.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Science-Water Research & Technology
·
2024
·
IF 3.5
Wei Wei,Shiqian Gao,Feiyue Qian,Chongjun Chen,Youyi Wu
Reduced graphene oxide modified CoFe2O4 (CoFe2O4/rGO) magnetic nanoparticles (MNPs) were synthesized by employing an in situ crystallization microwave irradiation method. The morphology and textural properties of CoFe2O4/rGO were characterized using SEM, TEM, BET/BJH and XPS. GO was reduced to rGO via the thermal catalysis of Co(acac)2, which contributed to expanding the effective microwave absorption bandwidth of carbon-based materials. The lattice stability of CoFe2O4 effectively improved Snoek's limit and protected the catalytic active site distribution of CoFe2O4/rGO nanoparticles. The CoFe2O4/rGO composites were selected as a heterogeneous catalyst to initiate the activation of peroxymonosulfate (PMS) for the generation of reactive oxygen species (ROS) and degrade 2-aminobenzothiazole (ABT) in a water sample. Under optimal conditions, the coupling of CoFe2O4/rGO and PMS can completely degrade ABT in aqueous solutions within 90 minutes. The effect of inorganic anions, metal cations and humic acid (HA) on the degradation efficiency of ABT was explored. Experimental results showed that the presence of HA and low concentrations of Cu2+ enhanced the process performance remarkably, while the addition of NO3−, SO42−, Zn2+, Cd2+ and high concentrations of Cu2+ suppressed the degradation of ABT. Meanwhile, the absence of Cl− and HCO3− presented no significant influence on the degradation. Radical quenching experiments indicated that SO4−·, ·OH and non-free radicals of 1O2 were involved in the CoFe2O4/rGO-PMS system, with the former two being the dominating radical species. A degradation efficiency of 100% was obtained when the proposed method was applied to the degradation of ABT in actual water samples. The CoFe2O4/rGO catalyst-activated PMS processes offered a reference to eliminate refractory organics in water.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）