Journal of Analysis and Testing
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2025
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IF 7
Wang Shuaishuai,Shen Chen,Wei Yuqing,Zhao Hongwei,Chen Jia,Zhang Bo,Qiu Hongdeng
Ion exchange chromatography (IEC) is one of the essential methods to separate rare earth elements (REEs), which relies on the stationary phase. In this study, a novel aromatic sulfonic acid-modified silica stationary phase (SCX-Tol) was synthesized by grafting aromatic sulfonic acid groups onto silica surfaces using 2-(4-chlorosulfonylphenyl)ethyl trichlorosilane as the functionalizing agent in toluene. The successful modification was confirmed by scanning electron microscopy (SEM), elemental analysis, X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR). The chromatographic performance of SCX-Tol was systematically evaluated and compared with two commercial ion-exchange columns. The results demonstrated the superior separation efficiency of SCX-Tol, particularly in the challenging separation of dysprosium (Dy) and yttrium (Y), which are traditionally difficult to resolve using chromatographic methods. The separation efficiency improved with an increasing density of sulfonic acid groups on the stationary phase surface. These findings indicate that SCX-Tol is a promising stationary phase for both the separation and quantification of REEs, offering the potential applications in separating and detecting REEs.
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JOURNAL OF APPLIED ELECTROCHEMISTRY
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2024
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IF 2.4
Zhou Yicheng,Dong Li,Liu Lin,Liu Chao,Zhang Xiaobo,Tong Zhiwei
Based on the layered semiconductor material potassium tantalum titanate, a novel layered nanocomposite, C 3 F 7 –Azo + /TiTaO 5 , was successfully synthesized using the stripping/recombination method. The surfactant trans-[2 -(2,2,3,3,4,4,4-heptafluorobutylamino) ethyl]-{2-[4-(4-hexylbenzene-azo)-phenoxy] ethyl}—dimethylammonium, abbreviated as C 3 F 7 –Azo + Br − , served as the active center. The resulting composite was utilized to modify a glassy carbon electrode (GCE), forming the composite modified electrode C 3 F 7 –Azo + /TiTaO 5 /GCE, which was employed for the electrochemical detection of nitrite. During the experiment, X-ray diffractometry, transmission electron microscopy, and energy dispersive spectroscopy were used to characterize the morphology, structure, and elemental composition of the prepared materials. The electrochemical properties of the modified electrode were evaluated through cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry. The results indicated that the composite electrode C 3 F 7 –Azo + /TiTaO 5 /GCE exhibited smaller electrochemical impedance (341.4 Ω) and a larger electrochemical active area (0.107 cm 2 ). It also displayed excellent sensitivity to nitrite, with a detection limit of 0.87 μM in the concentration range of 1.25–40.46 μM. Furthermore, the composite modified electrode C 3 F 7 –Azo + /TiTaO 5 /GCE demonstrated exceptional stability and anti-interference capability and effectively detected nitrite in real samples, such as river water, with recovery rates ranging from 97.83 to 99.12%. Graphical abstract
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Bone Research
·
2025
·Vol. 13
·期 1
·1-17
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IF 15
Ni Yuehan,Wu Jiamiao,Liu Fengqi,Yi Yating,Meng Xiangjiao,Gao Xiang,Xiao Luyi,Zhou Weiwei,Chen Zexi,Chu Peng,Xing Dan,Yuan Ye,Ding Donghui,Shen Ge,Yang Min,Wu Ronjie,Wang Ling,Melo Luiza Martins Nascentes,Lin Sien,Cheng Xiaoguang,Li Gang,Tasdogan Alpaslan,Ubellacker Jessalyn M.,Zhao Hu,Fang Shentong,Shen Bo
Tissue clearing combined with high-resolution confocal imaging is a cutting-edge approach for dissecting the three-dimensional (3D) architecture of tissues and deciphering cellular spatial interactions under physiological and pathological conditions. Deciphering the spatial interaction of leptin receptor-expressing (LepR + ) stromal cells with other compartments in the bone marrow is crucial for a deeper understanding of the stem cell niche and the skeletal tissue. In this study, we introduce an optimized protocol for the 3D analysis of skeletal tissues, enabling the visualization of hematopoietic and stromal cells, especially LepR + stromal cells, within optically cleared bone hemisections. Our method preserves the 3D tissue architecture and is extendable to other hematopoietic sites such as calvaria and vertebrae. The protocol entails tissue fixation, decalcification, and cryosectioning to reveal the marrow cavity. Completed within approximately 12 days, this process yields highly transparent tissues that maintain genetically encoded or antibody-stained fluorescent signals. The bone hemisections are compatible with diverse antibody labeling strategies. Confocal microscopy of these transparent samples allows for qualitative and quantitative image analysis using Aivia or Bitplane Imaris software, assessing a spectrum of parameters. With proper storage, the fluorescent signal in the stained and cleared bone hemisections remains intact for at least 2–3 months. This protocol is robust, straightforward to implement, and highly reproducible, offering a valuable tool for tissue architecture and cellular interaction studies.
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Chinese Medicine
·
2025
·Vol. 20
·期 1
·1-14
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IF 5.7
Jiang Feng,Tang Jiayang,Wei Xiaoqi,Pan Hai,Fan Xinyi,Zhang Peng,Guo Shuzhen
Background Dilated cardiomyopathy (DCM) stands as one of the most prevalent and severe causes of heart failure. Inflammation plays a pivotal role throughout the progression of DCM to heart failure, while age acts as a natural predisposing factor for all cardiovascular diseases. These two factors often interact, contributing to cardiac fibrosis, which is both a common manifestation and a pathogenic driver of adverse remodeling in DCM-induced heart failure.MethodBulk RNA-seq, single-cell RNA-seq, Mendelian randomization analysis, animal model construction, and BMP6 knockdown were utilized to identify and validate potential specific markers and targets for intervention in DCM heart failure.Results We found that DCM hearts exhibit pronounced inflammatory cell infiltration and fibrosis. Both bulk RNA-seq and single-cell RNA-seq analyses revealed aberrant BMP6 expression specifically in fibroblasts. The ROC curve underscores the high specificity of BMP6 in relation to DCM, while Mendelian randomization analysis further confirms BMP6 as a protective factor against DCM. Notably, BMP6 knockdown led to a decrease in SMAD6 expression and a marked elevation in COL1A1 expression levels, indicating its antifibrotic role.Conclusion BMP6 emerges as a promising biomarker for DCM, and its functional role in exerting an antifibrotic effect underscores its potential as a therapeutic target.
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MICROCHIMICA ACTA
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2025
·Vol. 192
·期 5
·1-14
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IF 5.3
Bu Ziyun,Si Qiankang,Lu Xiaolin,Wang Pei,Wu Dezhen,Mei Yingying
A novel monolithic capillary column (AuNPs@CMIP(poly-Chitosan)@capillary) with hybridized chiral molecular imprinted polymers (CMIPs) as stationary phase was prepared using S-metolachlor (S-MET) as template, chitosan as functional monomer, glutaraldehyde as cross-linker, and aminated-gold nanoparticles (NH 2 -AuNPs) as nanocarriers. The capillary electrochromatography (CEC) system constructed by AuNPs@CMIP(poly-Chitosan)@capillary achieved the efficient chiral separation of MET (resolution (Rs) = 9.83) as well as separation of its various structural analogs (Rs > 1.58). In addition, AuNPs@CMIP(poly-Chitosan)@capillary exhibits satisfactory reproducibility and stability. The relative standard deviations (RSDs) of migration times and Rs for intra-day, inter-day, and inter-column were all less than 6.1%, and no significant decline in separation performance was observed after 21 days of storage. NH 2 -AuNPs, with their large specific surface area and stable structure, can provide abundant binding sites and play a supporting role, reducing the deformation and collapse of CMIPs’ cavities, and further ensuring the structural stability and separation performance of the stationary phase. Finally, parameters such as imprinting factor and selectivity factor were calculated by adsorption experiments, and the CEC separation mechanism was discussed. The chitosan CMIPs-based capillary monolithic column developed in this work has rarely been reported and is an innovative attempt, providing a feasible and promising approach for the development of CEC chiral monolithic stationary phases. Graphical abstract
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Nature Communications
·
2020
·Vol. 11
·期 1
·1-13
·
IF 12.121
Gu Yuqing,He Chang,Zhang Yuqing,Lin Li,Thackray Benjamin David,Ye Jian
Anticounterfeiting labels based on physical unclonable functions (PUFs), as one of the powerful tools against counterfeiting, are easy to generate but difficult to duplicate due to inherent randomness. Gap-enhanced Raman tags (GERTs) with embedded Raman reporters show strong intensity enhancement and ultra-high photostability suitable for fast and repeated readout of PUF labels. Herein, we demonstrate a PUF label fabricated by drop-casting aqueous GERTs, high-speed read using a confocal Raman system, digitized through coarse-grained coding methods, and authenticated via pixel-by-pixel comparison. A three-dimensional encoding capacity of over 3 × 10 15051 can be achieved for the labels composed of ten types of GERTs with a mapping resolution of 2500 pixels and quaternary encoding of Raman intensity levels at each pixel. Authentication experiments have ensured the robustness and security of the PUF system, and the practical viability is demonstrated. Such PUF labels could provide a potential platform to realize unbreakable anticounterfeiting.
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Human Cell
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2025
·Vol. 38
·期 3
·1-15
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IF 3.1
Li Chenhao,Wang Xin,Shi Dongliang,Yang Meng,Yang Wenhua,Chen Liang
Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype characterized by high recurrence rates, low survival rates, and a lack of therapeutic targets. Regulatory Factor X5 (RFX5) is a critical transcription factor during tumor progression. However, the role of RFX5 involving breast cancer or TNBC has not been studied. This study obtained 60 tumor samples of TNBC for analysis and ascertained that RFX5 is linked with the severe stage. We constructed RFX5 knockdown and overexpression models involving TNBC cells. RFX5 overexpression enhanced TNBC cell proliferation by detecting cell vitality and replication of DNA and analyzing cell cycle data. RFX5 facilitated cell migration and invasion, which were determined by wound healing and Transwell assays. The anti-apoptotic RFX5 properties were confirmed with Hoechst staining and Annexin V/PI apoptosis assays. The Notch pathway was activated in TNBC, and Jagged canonical Notch ligand 1 (JAG1) could enhance TNBC growth and metastasis. RFX5 upregulation elevated JAG1 mRNA and protein levels. Chromatin immunoprecipitation and luciferase reporter assays indicated that RFX5 promoted the transcriptional activation of JAG1 by binding the promoter (− 1890/+ 15 or − 1359/+ 15 area). JAG1 knockdown reduced RFX5-induced expression of Notch signaling-related factors Notch1, NICD, and Hes1. This paper indicated that RFX5 is a transcription factor for JAG1 and established that RFX5 could activate the Notch pathway via transcriptional activation of JAG1 and promote TNBC progression. Targeting RFX5 could be a promising therapeutic approach against TNBC.
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Food and Bioprocess Technology
·
2024
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IF 5.3
Xu Hailin,Guo Yaru,Zhou Shufang,Wang Jiayi,Lu Futai,Wang Shuo,Deng Qiliang
Time and temperature are the crucial factors affecting the quality of perishable products. The device capable of recording temperature fluctuation and maintaining time has huge practical potential. In this research, a novel thermal history indicator (THI) was proposed based on covalent organic frameworks (COFs) with the irreversible color change performance. The color change and absorbance of the THI were investigated over a broad monitoring temperature range (− 22 ~ 55 ℃) and were closely correlated with its thermal accumulation over time and temperature. The activation energy ( E a ) of the THI was calculated to be 55.31 kJ mol −1 , which can be used to monitor the perishable products with deterioration reaction E a ranging from 30.31 to 80.31 kJ mol −1 . In addition, the THI also demonstrated the significant response to the environmental temperature fluctuations. Furthermore, the potential applications were evaluated by recording the changes of the THI gel and the total viable count (TVC) of food under the same conditions. The results indicated that the digital images of the colorimetric THI gel were correlated with TVC of food at 4 ℃ and 25 ℃, respectively. These results support that the proposed THI is potential for real-time, accurate, non-destructive, visually, and quantitatively monitoring food freshness during food supply chain distribution. Graphical Abstract
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BRITISH JOURNAL OF CANCER
·
2025
·
IF 6.8
Pu Yamin,Xu Fuyan,He Anqi,Li Ru,Wang Xiangxiu,Zhou Liang,Sun Hongbao,Zhang Yiwen,Xia Yong
Background Triple-negative breast cancer (TNBC) presents significant challenges due to its aggressive nature and high propensity for brain metastasis, often exhibiting resistance to standard treatments. In this study, we conducted a preliminary screening of potential therapeutic agents and identified chlorpromazine (CPZ) as a promising candidate for treating TNBC and its brain metastases. Methods The inhibitory activities of CPZ and its combination with several standard treatment drugs were evaluated in preclinical TNBC models. The mechanism of CPZ on TNBC was elucidated using TMT-labeled quantitative proteomics analysis. Results In vivo experiments demonstrated that CPZ robustly suppressed tumor growth and metastasis, particularly in lung and brain models. Importantly, CPZ enhanced the efficacy of standard therapeutic agents such as vinorelbine (NVB) and anti-PD-1 antibody. Mechanistically, CPZ induced G2/M phase arrest and triggered mitochondria-mediated intrinsic apoptosis in TNBC cells. Furthermore, CPZ triggered incomplete autophagy and activated PINK1-Parkin-mediated mitophagy. Inhibiting autophagy/mitophagy augmented CPZ’s anticancer effects, indicating these processes may have cell protective roles. Conclusions Our study highlights the dual function of CPZ in suppressing TNBC growth and metastasis, positioning it as a promising candidate for treating this aggressive cancer. Additionally, targeting autophagy/mitophagy may serve as an effective strategy to enhance anticancer therapies against TNBC.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 3
·1-13
·
IF 5.3
Liu Yaling,Zhang Yibo,Ye Changrui,He Yayi,Wang Shaopeng,Zhang Man,Fu Hao,Lu Shan,Wang Liwei
An electrochemical biosensor was constructed for quantitatively detecting Skeletonema costatum ( S. costatum ) by combining the electrode modification material (NC-Au) with a strand displacement reaction (SDR). The SDR process addresses the issues of steric hindrance and electrostatic repulsion resulting from the large size of genomic DNA. It enhances the efficiency of the interfacial hybridization reaction and endows the biosensor with remarkable sensitivity. The limit of detection (LOD) and limit of quantification (LOQ) were 33.43 fg/μL (831 cells/L) and 87.21 fg/μL (2112 cells/L), respectively. Additionally, the biosensor has demonstrated excellent accuracy compared to methods such as microscopy and ddPCR ( P > 0.05). Subsequent assessment of the adjacent waters of the Beibu Gulf using biosensors indicated a low risk of S. costatum red tide outbreaks in the region, which is consistent with the findings of the ecological survey. Therefore, we believe that this biosensor can provide a completely new idea for the dynamic monitoring and early warning of S. costatum red tide. Graphical abstract
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International Journal of Environmental Science and Technology
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2025
·
IF 3.4
Yu R.,Li P.
Bisphenol A (BPA) is a ubiquitous emerging organic contaminant with potential endocrine-disrupting effects. Inefficient removal of BPA from WWTPs are the main routes for it to enter the environment. This study evaluated efficiency of BPA in two full-scale wastewater treatment plants (WWTPs) employing anaerobic/anoxic/oxic-membrane bioreactor with hollow fiber ultrafiltration (AAO-MBR/UF) and multi-level anoxic/oxic activated sludge process (M-AO) with coagulation-flocculation (CF) process. According to the results, both processes achieved effective BPA removal, with AAO-MBR/UF demonstrating higher biodegradation efficiency. The CF process made further removal of BPA after M-AO process. Additionally, we investigated the antagonistic effect of chlorogenic acid (CGA) on BPA cardiotoxicity in zebrafish. CGA significantly mitigated BPA-induced developmental and cardiac toxicity, likely through competitive binding to myosin. These findings highlight the potential removal and the protective role of CGA against BPA cardiotoxicity, offering valuable insights for environmental risk management and potential therapeutic applications.
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INFLAMMOPHARMACOLOGY
·
2025
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IF 5.3
Li Lirong,Huang Rui,Li Wenwen,Yao Dengjie,Zhang Beibei,Li Mengna,Luo Shunan,Qin Yu,Zhang Wenwen
Camellia nitidissima Chi (CNC), whose main composition was gallic acid, catechin, procyanidin-gallate, apigenin-pentosyl-glucoside, vitexin, cyanidin-3-o-glucoside, myricitrin-glucoside, gallocatechin-gallate, epicatechin and rutin, posses sound anti-inflammatory and antioxidant effects. This study aimed to explore the protective effects and mechanisms of the alcoholic extract of CNC on dextran sulfate sodium-induced ulcerative colitis. CNC effectively maintained weight and colon length, and significantly ameliorated colonic histopathological damage. Furthermore, CNC mitigated colitis-induced oxidative stress and exhibited anti-inflammatory properties in UC mice by reducing levels of myeloperoxidase (MPO) and malondialdehyde (MDA), decreasing the production of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). In addition, CNC downregulated the protein expression levels of TLR4, p-NF-κBP65, and p-IκBα of the inflammation-related TLR4/NF-κB signaling pathways. Validation of the molecular docking results also revealed that rutin, the most abundant compound in CNC, interacts with TLR4 and NF-κB protein mainly through hydrogen bonds. CNC also promoted the proliferation of Lactobacillus and Bifidobacterium while reducing the number of Enterococcus , E. coli , Bacteroides, and Peptococcus in mice to improve the intestinal environment and alleviate colitis. CNC inhibited TLR4/NF-κB signaling pathways to relieve inflammation and oxidative damage on UC, is a potential development of natural medicine for UC.
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